Transcriptome profile analysis of mucosal immune responses in the gill of Takifugu rubripes infected with Vibrio harveyi

IF 2.2 2区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-06-20 DOI:10.1016/j.cbd.2025.101570

Dong Sheng , Hongli Ma , Xu Ren, Yuxiao Liu, Xiaolong Ding, Qinghua Yu, Weijia Zhou, Shigen Ye, Zhixin Guo

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Abstract

Vibrio harveyi is the main pathogen causing significant economic losses in Takifugu rubripes farming. The early immune response to V. harveyi infection in T. rubripes was investigated using Illumina NovaSeq 6000 technology. A total of 4720 differentially expressed genes were identified in the gill transcriptome at 0, 3, and 24 h post-infection compared to the control group. Pathways associated with mucosal immunity, such as cytokine-cytokine receptor interaction, cell adhesion molecules (CAMs), and the NOD-like receptor signaling pathway, were significantly enriched. Specifically, the results implied the cytokine-cytokine receptor interaction pathway is crucial in mediating immune responses. The cooperative interplay between Ccr7 and Cxcl13 may enhance pathogen resistance during the early mucosal immune phase in T. rubripes. These findings provide valuable insights into the early mucosal immune response to V. harveyi infection in T. rubripes.

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感染哈氏弧菌的红鳉鱼鳃黏膜免疫应答的转录组分析

哈威氏弧菌是造成红鳍东方鲀养殖重大经济损失的主要病原体。采用Illumina NovaSeq 6000技术研究了红带绦虫对哈维氏弧菌感染的早期免疫反应。与对照组相比，感染后0、3和24小时，在鳃转录组中共鉴定出4720个差异表达基因。与粘膜免疫相关的途径，如细胞因子-细胞因子受体相互作用、细胞粘附分子（CAMs）和nod样受体信号通路显著富集。具体而言，结果表明细胞因子-细胞因子受体相互作用途径在介导免疫反应中至关重要。Ccr7和Cxcl13的协同相互作用可能增强红带绦虫早期粘膜免疫阶段的病原体抗性。这些发现提供了宝贵的见解，早期黏膜免疫反应，以V.哈维感染红带t。

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来源期刊

Comparative Biochemistry and Physiology D-Genomics & Proteomics 生物-生化与分子生物学

CiteScore

5.10

自引率

3.30%

发文量

审稿时长

33 days

期刊介绍： Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.