Current Topics in Developmental Biology最新文献_第6页

Human kidney organoids for modeling the development of different diseases. 人类肾脏类器官用于模拟不同疾病的发展。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2025-02-06 DOI: 10.1016/bs.ctdb.2024.12.001

Elena Ceccotti, Armina Semnani, Benedetta Bussolati, Stefania Bruno

{"title":"Human kidney organoids for modeling the development of different diseases.","authors":"Elena Ceccotti, Armina Semnani, Benedetta Bussolati, Stefania Bruno","doi":"10.1016/bs.ctdb.2024.12.001","DOIUrl":"https://doi.org/10.1016/bs.ctdb.2024.12.001","url":null,"abstract":"The increasing incidence of kidney diseases has highlighted the need for in vitro experimental models to mimic disease development and to test new therapeutic approaches. Traditional two-dimensional in vitro experimental models are not fully able to recapitulate renal diseases. Instead, kidney organoids represent three-dimensional models that better mimic the human organ from both structural and functional points of view. Human pluripotent stem cells (PSCs), both embryonic and induced, are ideal sources for generating renal organoids. These organoids contain all renal cell types and the protocols to differentiate PSCs into renal organoids consist of three different stages that recapitulate embryonic development: mesodermal induction, nephron progenitor formation, and nephron differentiation. Recently it has been establish a renal organoid model where collecting ducts are also present. In this case, the presence of ureteric bud progenitor cells is essential. Renal organoids are particularly useful for studying genetic diseases, by introducing the specific mutations in PSCs by genome editing or generating organoids from patient-derived PSCs. Moreover, renal organoids represent promising models in toxicology studies and testing new therapeutic approaches. Renal organoids can be established also from adult stem cells. This type of organoid, named tubuloid, is composed only of epithelial cells and recapitulates the tissue repair process. The tubuloids can be generated from adult stem or progenitor cells, obtained from renal biopsies or urine, and are promising in vitro models for studying tubular functions, diseases, and regeneration. Tubuloids can be derived from patients and permit the study of genetic diseases, performing personalized drug screening and modeling renal pathologies.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"163 ","pages":"364-393"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From neural crest migration to the onset of gangliogenesis. 从神经嵴迁移到神经节发生的开始。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2025-03-13 DOI: 10.1016/bs.ctdb.2025.02.003

Hugo A Urrutia, Marianne E Bronner

{"title":"From neural crest migration to the onset of gangliogenesis.","authors":"Hugo A Urrutia, Marianne E Bronner","doi":"10.1016/bs.ctdb.2025.02.003","DOIUrl":"https://doi.org/10.1016/bs.ctdb.2025.02.003","url":null,"abstract":"The neural crest is a highly migratory and multipotent cell population that contributes to many defining features of vertebrates. As a uniquely vertebrate cell type, the neural crest is an excellent model for studying cell lineage and diversification during embryonic development because of its multipotency, motility, and capacity to form a plethora of derivatives. Neural crest cells migrate extensively throughout the body and contribute to many of the defining features of vertebrate embryos, including the craniofacial skeleton, most of the peripheral nervous system and pigmentation of the skin. What guides their migration and subsequent formation of discrete structures? Interactions between neural crest cells and their environment, including other cell types like placode cells, play a major role in guiding their migration and condensation into numerous derivatives. In this review, we discuss aspects of neural crest induction, migration and axial level differences, highlighting what is currently known regarding molecular cues that govern their formation, migratory behavior, and differentiation as they reach their final destinations. We particularly focus on formation of cranial sensory ganglia. New technologies are playing an important role in furthering our understanding of the molecular mechanisms underlying neural crest migration and what leads to cessation of their movement and onset of differentiation.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"164 ","pages":"67-108"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144509536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outside influences: The impact of extracellular matrix mechanics on cell migration. 外界影响：细胞外基质力学对细胞迁移的影响。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2025-01-28 DOI: 10.1016/bs.ctdb.2025.01.003

Ronen Zaidel-Bar, Priti Agarwal

{"title":"Outside influences: The impact of extracellular matrix mechanics on cell migration.","authors":"Ronen Zaidel-Bar, Priti Agarwal","doi":"10.1016/bs.ctdb.2025.01.003","DOIUrl":"https://doi.org/10.1016/bs.ctdb.2025.01.003","url":null,"abstract":"\"No cell is an island\" - highlights the interconnectedness of cellular behavior and the extracellular matrix (ECM). Cell migration is inherently contextual, as cells navigate and adapt to their environments, reshaping the ECM while being influenced by its properties. This review focuses on the mechanical characteristics of the ECM-specifically its architecture, porosity, dynamics, and stiffness-and how these attributes affect cell behavior and migration strategies. We discuss how the mechanical properties are modulated by the composition and arrangement of ECM components and the role of enzymatic activities, including crosslinking and matrix metalloproteinases. By presenting examples from vertebrate and invertebrate developmental models, we demonstrate how ECM mechanics dictate cell migration at various biological scales. Additionally, we examine the importance of cell-matrix adhesions in regulating migration speed and direction. While in vitro studies have advanced our understanding of the molecular mechanisms at play, significant questions persist regarding the regulation of cell migration by ECM mechanics in vivo. Ultimately, this synthesis aims to illuminate the complexities of cell-ECM mechanical interactions, pointing the way for future research that may unveil novel insights into how ECM mechanics influences cell migration during development and disease.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"164 ","pages":"29-65"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144509537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell migration in development - Lessons from germ cells. 发育中的单细胞迁移——来自生殖细胞的教训。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2025-01-27 DOI: 10.1016/bs.ctdb.2025.01.002

Jan Schick, Laura Ermlich, Lucas Kühl, Dennis Hoffmann, Erez Raz

引用次数: 0

No transcription, no problem: Protein phosphorylation changes and the transition from oocyte to embryo. 没有转录，没有问题：蛋白磷酸化的改变和从卵母细胞到胚胎的转变。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2025-02-18 DOI: 10.1016/bs.ctdb.2025.01.001

Jonathon M Thomalla, Mariana F Wolfner

{"title":"No transcription, no problem: Protein phosphorylation changes and the transition from oocyte to embryo.","authors":"Jonathon M Thomalla, Mariana F Wolfner","doi":"10.1016/bs.ctdb.2025.01.001","DOIUrl":"10.1016/bs.ctdb.2025.01.001","url":null,"abstract":"Although mature oocytes are arrested in a differentiated state, they are provisioned with maternally-derived macromolecules that will start embryogenesis. The transition to embryogenesis, called 'egg activation', occurs without new transcription, even though it includes major cell changes like completing stalled meiosis, translating stored mRNAs, cytoskeletal remodeling, and changes to nuclear architecture. In most animals, egg activation is triggered by a rise in free calcium in the egg's cytoplasm, but we are only now beginning to understand how this induces the egg to transition to totipotency and proliferation. Here, we discuss the model that calcium-dependent protein kinases and phosphatases modify the phosphorylation landscape of the maternal proteome to activate the egg. We review recent phosphoproteomic mass spectrometry analyses that revealed broad phospho-regulation during egg activation, both in number of phospho-events and classes of regulated proteins. Our interspecies comparisons of these proteins pinpoints orthologs and protein families that are phospho-regulated in activating eggs, many of which function in hallmark events of egg activation, and others whose regulation and activity warrant further study. Finally, we discuss key phospho-regulating enzymes that may act apically or as intermediates in the phosphorylation cascades during egg activation. Knowing the regulators, targets, and effects of phospho-regulation that cause an egg to initiate embryogenesis is crucial at both fundamental and applied levels for understanding female fertility, embryo development, and cell-state transitions.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"162 ","pages":"165-205"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Satellite cells in the growth and maintenance of muscle. 卫星细胞在肌肉生长和维持过程中的作用。

2区生物学

Current Topics in Developmental Biology Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.1016/bs.ctdb.2024.01.020

John F Bachman, Joe V Chakkalakal

引用次数: 0

Muscle stem cells as immunomodulator during regeneration. 肌肉干细胞作为再生过程中的免疫调节剂

2区生物学

Current Topics in Developmental Biology Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.1016/bs.ctdb.2024.01.010

H Rex Xu, Victor V Le, Stephanie N Oprescu, Shihuan Kuang

引用次数: 0

Macrophage lineages in heart development and regeneration. 心脏发育和再生过程中的巨噬细胞系

2区生物学

Current Topics in Developmental Biology Pub Date : 2024-01-01 Epub Date: 2024-02-24 DOI: 10.1016/bs.ctdb.2024.01.004

Na Xu, Brittany A Gonzalez, Katherine E Yutzey

引用次数: 0

The cardiac conduction system: History, development, and disease. 心脏传导系统：历史、发展和疾病。

2区生物学

Current Topics in Developmental Biology Pub Date : 2024-01-01 Epub Date: 2024-03-06 DOI: 10.1016/bs.ctdb.2024.02.006

Carissa Lee, Sidra Xu, Tahmina Samad, William R Goodyer, Alireza Raissadati, Paul Heinrich, Sean M Wu

引用次数: 0

The genetics of cardiomyocyte polyploidy. 心肌细胞多倍体的遗传学。

2区生物学

Current Topics in Developmental Biology Pub Date : 2024-01-01 Epub Date: 2024-02-06 DOI: 10.1016/bs.ctdb.2024.01.008

Tyler Buddell, Alexandra L Purdy, Michaela Patterson

{"title":"The genetics of cardiomyocyte polyploidy.","authors":"Tyler Buddell, Alexandra L Purdy, Michaela Patterson","doi":"10.1016/bs.ctdb.2024.01.008","DOIUrl":"10.1016/bs.ctdb.2024.01.008","url":null,"abstract":"The regulation of ploidy in cardiomyocytes is a complex and tightly regulated aspect of cardiac development and function. Cardiomyocyte ploidy can range from diploid (2N) to 8N or even 16N, and these states change during key stages of development and disease progression. Polyploidization has been associated with cellular hypertrophy to support normal growth of the heart, increased contractile capacity, and improved stress tolerance in the heart. Conversely, alterations to ploidy also occur during cardiac pathogenesis of diseases, such as ischemic and non-ischemic heart failure and arrhythmia. Therefore, understanding which genes control and modulate cardiomyocyte ploidy may provide mechanistic insight underlying cardiac growth, regeneration, and disease. This chapter summarizes the current knowledge regarding the genes involved in the regulation of cardiomyocyte ploidy. We discuss genes that have been directly tested for their role in cardiomyocyte polyploidization, as well as methodologies used to identify ploidy alterations. These genes encode cell cycle regulators, transcription factors, metabolic proteins, nuclear scaffolding, and components of the sarcomere, among others. The general physiological and pathological phenotypes in the heart associated with the genetic manipulations described, and how they coincide with the respective cardiomyocyte ploidy alterations, are further discussed in this chapter. In addition to being candidates for genetic-based therapies for various cardiac maladies, these genes and their functions provide insightful evidence regarding the purpose of widespread polyploidization in cardiomyocytes.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"156 ","pages":"245-295"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140332343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0