Fungal Genetics and Biology最新文献_第7页

Two ferrous iron transporter-like proteins independently participate in asexual development under iron limitation and virulence in Beauveria bassiana 两种类似铁转运体的亚铁蛋白独立参与铁限制条件下的无性发育和巴斯氏菌的毒力。

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2024-06-09 DOI: 10.1016/j.fgb.2024.103908

Jin-Li Ding, Ming-Guang Feng, Sheng-Hua Ying

{"title":"Two ferrous iron transporter-like proteins independently participate in asexual development under iron limitation and virulence in Beauveria bassiana","authors":"Jin-Li Ding, Ming-Guang Feng, Sheng-Hua Ying","doi":"10.1016/j.fgb.2024.103908","DOIUrl":"10.1016/j.fgb.2024.103908","url":null,"abstract":"<div>Reductive assimilation pathway involves ferric reductase and ferrous iron transporter, which is integral for fungal iron acquisition. A family of ferric reductase-like proteins has been functionally characterized in the filamentous entomopathogenic fungus Beauveria bassiana. In this investigation, two ferrous iron transporter-like proteins (Ftr) were functionally annotated in B. bassiana. BbFtr1 and BbFtr2 displayed high similarity in structure and were associated with the plasma and nuclear membrane. Their losses had no negatively influence on fungal growth on various nutrients and development under the iron-replete condition. Single mutants of BbFTR1 and BbFTR2 displayed the iron-availability dependent developmental defects, and double mutant exhibited the significantly impaired developmental potential under the iron-limited conditions. In insect bioassay, the double mutant also showed the weaker virulence than either of two single disruption mutants. These results suggested that two ferrous iron transporter-like proteins function independently in fungal physiologies under the iron-deficient condition. Intriguingly, a bZIP transcription factor BbHapX was required for expression of BbFTR1 and BbFTR2 under iron-depleted conditions. This study enhances our understanding of the iron uptake system in the filamentous entomopathogenic fungi.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"173 ","pages":"Article 103908"},"PeriodicalIF":3.0,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141302139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of two genes essential for basidiospore formation during the postmeiotic stages in Pleurotus ostreatus 鉴定出两种对黑孢蘑菇减数分裂后基部孢子形成至关重要的基因。

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2024-06-01 DOI: 10.1016/j.fgb.2024.103890

Takeshi Kobukata , Takehito Nakazawa , Fuga Yamasaki , Junko Sugano , Minji Oh , Moriyuki Kawauchi , Masahiro Sakamoto , Yoichi Honda

{"title":"Identification of two genes essential for basidiospore formation during the postmeiotic stages in Pleurotus ostreatus","authors":"Takeshi Kobukata , Takehito Nakazawa , Fuga Yamasaki , Junko Sugano , Minji Oh , Moriyuki Kawauchi , Masahiro Sakamoto , Yoichi Honda","doi":"10.1016/j.fgb.2024.103890","DOIUrl":"10.1016/j.fgb.2024.103890","url":null,"abstract":"<div>A sporeless strain is an important breeding target in the mushroom industry. However, basidiospore production in the oyster mushroom Pleurotus ostreatus has been shown to be impaired by single-gene mutations in only two meiosis-related genes, mer3 and msh4. This study proposed a strategy for identifying the genes essential for basidiospore formation after meiotic division to determine new targets for molecular breeding. RNA-seq analysis was performed to identify P. ostreatus genes that are specifically expressed in the gill tissue of fruiting bodies, where basidiospore formation occurs. Transcriptome data during fruiting development of Coprinopsis cinerea, in which the meiotic steps progress synchronously, were then used to identify genes that are active in the postmeiotic stages. Based on these comparative analyses, five P. ostreatus genes were identified. Plasmids containing expression cassettes for hygromycin B-resistance screening, Cas9, and single-guide RNA targeting each gene were introduced into the protoplasts of dikaryotic strain, PC9×#64, to generate dikaryotic gene disruptants. Among the obtained transformants, three dikaryotic pcl1 disruptants and two cro6c disruptants did not produce basidiospores. Microscopic analyses indicated that spore formation was arrested at particular stages in these gene disruptants. These results indicate that these two genes are essential for mature spore formation in this fungus.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"172 ","pages":"Article 103890"},"PeriodicalIF":3.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140177851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The porcine skin microbiome exhibits broad fungal antagonism 猪皮肤微生物群表现出广泛的真菌拮抗作用。

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2024-05-28 DOI: 10.1016/j.fgb.2024.103898

Karinda F. De La Cruz , Elizabeth C. Townsend , J.Z. Alex Cheong , Rauf Salamzade , Aiping Liu , Shelby Sandstrom , Evelin Davila , Lynda Huang , Kayla H. Xu , Sherrie Y. Wu , Jennifer J. Meudt , Dhanansayan Shanmuganayagam , Angela L.F. Gibson , Lindsay R. Kalan

{"title":"The porcine skin microbiome exhibits broad fungal antagonism","authors":"Karinda F. De La Cruz , Elizabeth C. Townsend , J.Z. Alex Cheong , Rauf Salamzade , Aiping Liu , Shelby Sandstrom , Evelin Davila , Lynda Huang , Kayla H. Xu , Sherrie Y. Wu , Jennifer J. Meudt , Dhanansayan Shanmuganayagam , Angela L.F. Gibson , Lindsay R. Kalan","doi":"10.1016/j.fgb.2024.103898","DOIUrl":"10.1016/j.fgb.2024.103898","url":null,"abstract":"<div>The skin and its microbiome function to protect the host from pathogen colonization and environmental stressors. In this study, using the Wisconsin Miniature Swine™ model, we characterize the porcine skin fungal and bacterial microbiomes, identify bacterial isolates displaying antifungal activity, and use whole-genome sequencing to identify biosynthetic gene clusters encoding for secondary metabolites that may be responsible for the antagonistic effects on fungi. Through this comprehensive approach of paired microbiome sequencing with culturomics, we report the discovery of novel species of Corynebacterium and Rothia. Further, this study represents the first comprehensive evaluation of the porcine skin mycobiome and the evaluation of bacterial-fungal interactions on this surface. Several diverse bacterial isolates exhibit potent antifungal properties against opportunistic fungal pathogens in vitro. Genomic analysis of inhibitory species revealed a diverse repertoire of uncharacterized biosynthetic gene clusters suggesting a reservoir of novel chemical and biological diversity. Collectively, the porcine skin microbiome represents a potential unique source of novel antifungals.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"173 ","pages":"Article 103898"},"PeriodicalIF":3.0,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1087184524000355/pdfft?md5=ef6ac510593c67a2857fc7e834d28d80&pid=1-s2.0-S1087184524000355-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141180118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of endogenous UDP-glucosyltransferase in endophyte Phomopsis liquidambaris reduces deoxynivalenol contamination in wheat 表达内生菌 Phomopsis liquidambaris 中的内源性 UDP-葡萄糖基转移酶可减少小麦中的脱氧雪腐镰刀菌烯醇污染。

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2024-05-25 DOI: 10.1016/j.fgb.2024.103899

Meng-Qian Zhang , Zhi Yang , Yu-Xin Dong , Ya-Li Zhu , Xin-Yi Chen , Chuan-Chao Dai , Zhan Zhichun , Yan-Zhen Mei

{"title":"Expression of endogenous UDP-glucosyltransferase in endophyte Phomopsis liquidambaris reduces deoxynivalenol contamination in wheat","authors":"Meng-Qian Zhang , Zhi Yang , Yu-Xin Dong , Ya-Li Zhu , Xin-Yi Chen , Chuan-Chao Dai , Zhan Zhichun , Yan-Zhen Mei","doi":"10.1016/j.fgb.2024.103899","DOIUrl":"10.1016/j.fgb.2024.103899","url":null,"abstract":"<div>Fusarium head blight is a devastating disease that causes severe yield loses and mycotoxin contamination in wheat grain. Additionally, balancing the trade-off between wheat production and disease resistance has proved challenging. This study aimed to expand the genetic tools of the endophyte Phomopsis liquidambaris against Fusarium graminearum. Specifically, we engineered a UDP-glucosyltransferase-expressing P. liquidambaris strain (PL-UGT) using ADE1 as a selection marker and obtained a deletion mutant using an inducible promoter that drives Cas9 expression. Our PL-UGT strain converted deoxynivalenol (DON) into DON-3-G in vitro at a rate of 71.4 % after 36 h. DON inactivation can be used to confer tolerance in planta. Wheat seedlings inoculated with endophytic strain PL-UGT showed improved growth compared with those inoculated with wildtype P. liquidambaris. Strain PL-UGT inhibited the growth of Fusarium graminearum and reduced infection rate to 15.7 %. Consistent with this finding, DON levels in wheat grains decreased from 14.25 to 0.56 μg/g when the flowers were pre-inoculated with PL-UGT and then infected with F. graminearum. The expression of UGT in P. liquidambaris was nontoxic and did not inhibit plant growth. Endophytes do not enter the seeds nor induce plant disease, thereby representing a novel approach to fungal disease control.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"173 ","pages":"Article 103899"},"PeriodicalIF":3.0,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141158033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anaerobic fungi contain abundant, diverse, and transcriptionally active Long Terminal Repeat retrotransposons 厌氧真菌含有丰富、多样和转录活跃的长末端重复反转座子。

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2024-05-13 DOI: 10.1016/j.fgb.2024.103897

Tejas A. Navaratna , Nabil Alansari , Amy R. Eisenberg , Michelle A. O’Malley

{"title":"Anaerobic fungi contain abundant, diverse, and transcriptionally active Long Terminal Repeat retrotransposons","authors":"Tejas A. Navaratna , Nabil Alansari , Amy R. Eisenberg , Michelle A. O’Malley","doi":"10.1016/j.fgb.2024.103897","DOIUrl":"10.1016/j.fgb.2024.103897","url":null,"abstract":"<div>Long Terminal Repeat (LTR) retrotransposons are a class of repetitive elements that are widespread in the genomes of plants and many fungi. LTR retrotransposons have been associated with rapidly evolving gene clusters in plants and virulence factor transfer in fungal-plant parasite-host interactions. We report here the abundance and transcriptional activity of LTR retrotransposons across several species of the early-branching Neocallimastigomycota, otherwise known as the anaerobic gut fungi (AGF). The ubiquity of LTR retrotransposons in these genomes suggests key evolutionary roles in these rumen-dwelling biomass degraders, whose genomes also contain many enzymes that are horizontally transferred from other rumen-dwelling prokaryotes. Up to 10% of anaerobic fungal genomes consist of LTR retrotransposons, and the mapping of sequences from LTR retrotransposons to transcriptomes shows that the majority of clusters are transcribed, with some exhibiting expression greater than 104 reads per kilobase million mapped reads (rpkm). Many LTR retrotransposons are strongly differentially expressed upon heat stress during fungal cultivation, with several exhibiting a nearly three-log10 fold increase in expression, whereas growth substrate variation modulated transcription to a lesser extent. We show that some LTR retrotransposons contain carbohydrate-active enzymes (CAZymes), and the expansion of CAZymes within genomes and among anaerobic fungal species may be linked to retrotransposon activity. We further discuss how these widespread sequences may be a source of promoters and other parts towards the bioengineering of anaerobic fungi.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"172 ","pages":"Article 103897"},"PeriodicalIF":3.0,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1087184524000343/pdfft?md5=d633c3c7df714a43c4efd0404ec124da&pid=1-s2.0-S1087184524000343-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140946541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Botrytis cinerea detoxifies the sesquiterpenoid phytoalexin rishitin through multiple metabolizing pathways 灰葡萄孢菌通过多种代谢途径解毒倍半萜类植物雌激素利西汀

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2024-04-27 DOI: 10.1016/j.fgb.2024.103895

Abriel Salaria Bulasag , Akira Ashida , Atsushi Miura , Sreynich Pring , Teruhiko Kuroyanagi , Maurizio Camagna , Aiko Tanaka , Ikuo Sato , Sotaro Chiba , Makoto Ojika , Daigo Takemoto

{"title":"Botrytis cinerea detoxifies the sesquiterpenoid phytoalexin rishitin through multiple metabolizing pathways","authors":"Abriel Salaria Bulasag , Akira Ashida , Atsushi Miura , Sreynich Pring , Teruhiko Kuroyanagi , Maurizio Camagna , Aiko Tanaka , Ikuo Sato , Sotaro Chiba , Makoto Ojika , Daigo Takemoto","doi":"10.1016/j.fgb.2024.103895","DOIUrl":"https://doi.org/10.1016/j.fgb.2024.103895","url":null,"abstract":"<div>Botrytis cinerea is a necrotrophic pathogen that infects across a broad range of plant hosts, including high-impact crop species. Its generalist necrotrophic behavior stems from its ability to detoxify structurally diverse phytoalexins. The current study aims to provide evidence of the ability of B. cinerea to tolerate the sesquiterpenoid phytoalexin rishitin, which is produced by potato and tomato. While the growth of potato pathogens Phytophthora infestans (late blight) and Alternaria solani (early blight) was severely inhibited by rishitin, B. cinerea was tolerant to rishitin. After incubation of rishitin with the mycelia of B. cinerea, it was metabolized to at least six oxidized forms. Structural analysis of these purified rishitin metabolites revealed a variety of oxidative metabolism including hydroxylation at C7 or C12, ketone formation at C5, and dihydroxylation at the 10,11-olefin. Six rishitin metabolites showed reduced toxicity to P. infestans and A. solani, indicating that B. cinerea has at least 5 distinct enzymatic reactions to detoxify rishitin. Four host-specialized phytopathogenic Botrytis species, namely B. elliptica, B. allii, B. squamosa, and B. tulipae also had at least a partial ability to metabolize rishitin as B. cinerea, but their metabolic capacity was significantly weaker than that of B. cinerea. These results suggest that the ability of B. cinerea to rapidly metabolize rishitin through multiple detoxification mechanisms could be critical for its pathogenicity in potato and tomato.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"172 ","pages":"Article 103895"},"PeriodicalIF":3.0,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140815554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Plasmodiophora brassicae Golgi-localized UPF0016 protein PbGDT1 mediates calcium but not manganese transport in yeast and Nicotiana benthamiana 黄铜质菌高尔基定位的 UPF0016 蛋白 PbGDT1 在酵母和烟草中介导钙而非锰的运输

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2024-04-24 DOI: 10.1016/j.fgb.2024.103896

Md Musharaf Hossain, Cresilda Vergara Alinapon, Christopher D. Todd, Yangdou Wei, Peta C. Bonham-Smith

{"title":"The Plasmodiophora brassicae Golgi-localized UPF0016 protein PbGDT1 mediates calcium but not manganese transport in yeast and Nicotiana benthamiana","authors":"Md Musharaf Hossain, Cresilda Vergara Alinapon, Christopher D. Todd, Yangdou Wei, Peta C. Bonham-Smith","doi":"10.1016/j.fgb.2024.103896","DOIUrl":"https://doi.org/10.1016/j.fgb.2024.103896","url":null,"abstract":"<div>Manganese and calcium homeostasis and signalling, in eukaryotic organisms, are regulated through membrane located pumps, channels and exchangers, including the Mn2+/Ca2+ uncharacterized protein family 0016 (UPF0016). Here we show that Plasmodiophora brassicae PbGDT1 is a member of the UPF0016 and an ortholog of Saccharomyces cerevisiae Gdt1p (GCR Dependent Translation Factor 1) protein involved in manganese homeostasis as well as the calcium mediated stress response in yeast. PbGDT1 complemented the ScGdt1p and ScPMR1 (Ca2+ ATPase) double null mutant under elevated calcium stress but not under elevated manganese conditions. In both yeast and Nicotiana benthamiana, PbGDT1 localizes to the Golgi apparatus, with additional ER association in N. benthamiana. Expression of PbGDT1 in N. benthamiana, suppresses BAX-triggered cell death, further highlighting the importance of calcium homeostasis in maintaining cell physiology and integrity in a stress environment.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"172 ","pages":"Article 103896"},"PeriodicalIF":3.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1087184524000331/pdfft?md5=aa96f9f487f203180bf21a2666e98faf&pid=1-s2.0-S1087184524000331-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140815553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional analysis of basidiomycete specific chitin synthase genes in the agaricomycete fungus Pleurotus ostreatus 姬松茸中基生菌特异性几丁质合成酶基因的功能分析

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2024-04-23 DOI: 10.1016/j.fgb.2024.103893

Kim Schiphof , Moriyuki Kawauchi , Kenya Tsuji , Akira Yoshimi , Chihiro Tanaka , Takehito Nakazawa , Yoichi Honda

{"title":"Functional analysis of basidiomycete specific chitin synthase genes in the agaricomycete fungus Pleurotus ostreatus","authors":"Kim Schiphof , Moriyuki Kawauchi , Kenya Tsuji , Akira Yoshimi , Chihiro Tanaka , Takehito Nakazawa , Yoichi Honda","doi":"10.1016/j.fgb.2024.103893","DOIUrl":"https://doi.org/10.1016/j.fgb.2024.103893","url":null,"abstract":"<div>Chitin is an essential structural component of fungal cell walls composed of transmembrane proteins called chitin synthases (CHSs), which have a large range of reported effects in ascomycetes; however, are poorly understood in agaricomycetes. In this study, evolutionary and molecular genetic analyses of chs genes were conducted using genomic information from nine ascomycete and six basidiomycete species. The results support the existence of seven previously classified chs clades and the discovery of three novel basidiomycete-specific clades (BI–BIII). The agaricomycete fungus Pleurotus ostreatus was observed to have nine putative chs genes, four of which were basidiomycete-specific. Three of these basidiomycete specific genes were disrupted in the P. ostreatus 20b strain (ku80 disruptant) through homologous recombination and transformants were obtained (Δchsb2, Δchsb3, and Δchsb4). Despite numerous transformations Δchsb1 was unobtainable, suggesting disruption of this gene causes a crucial negative effect in P. ostreatus. Disruption of these chsb2–4 genes caused sparser mycelia with rougher surfaces and shorter aerial hyphae. They also caused increased sensitivity to cell wall and membrane stress, thinner cell walls, and overexpression of other chitin and glucan synthases. These genes have distinct roles in the structural formation of aerial hyphae and cell walls, which are important for understanding basidiomycete evolution in filamentous fungi.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"172 ","pages":"Article 103893"},"PeriodicalIF":3.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1087184524000306/pdfft?md5=3cf19418ad1f7cfd763fec32c688e240&pid=1-s2.0-S1087184524000306-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140644492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors 黑曲霉 FlbA 的多效应表型部分是由其下游调控的七个转录因子的活性所解释的

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2024-04-23 DOI: 10.1016/j.fgb.2024.103894

Xiaoyi Chen , Juan P. Moran Torres , Peter Jan Vonk , J. Mirjam A. Damen , Karli R. Reiding , Jan Dijksterhuis , Luis G. Lugones , Han A.B. Wösten

{"title":"The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors","authors":"Xiaoyi Chen , Juan P. Moran Torres , Peter Jan Vonk , J. Mirjam A. Damen , Karli R. Reiding , Jan Dijksterhuis , Luis G. Lugones , Han A.B. Wösten","doi":"10.1016/j.fgb.2024.103894","DOIUrl":"https://doi.org/10.1016/j.fgb.2024.103894","url":null,"abstract":"<div>Inactivation of flbA in Aspergillus niger results in thinner cell walls, increased cell lysis, abolished sporulation, and an increased secretome complexity. A total of 36 transcription factor (TF) genes are differentially expressed in ΔflbA. Here, seven of these genes (abaA, aslA, aslB, azf1, htfA, nosA, and srbA) were inactivated. Inactivation of each of these genes affected sporulation and, with the exception of abaA, cell wall integrity and protein secretion. The impact on secretion was strongest in the case of ΔaslA and ΔaslB that showed increased pepsin, cellulase, and amylase activity. Biomass was reduced of agar cultures of ΔabaA, ΔaslA, ΔnosA, and ΔsrbA, while biomass was higher in liquid shaken cultures of ΔaslA and ΔaslB. The ΔaslA and ΔhtfA strains showed increased resistance to H2O2, while ΔaslB was more sensitive to this reactive oxygen species. Together, inactivation of the seven TF genes impacted biomass formation, sporulation, protein secretion, and stress resistance, and thereby these genes explain at least part of the pleiotropic phenotype of ΔflbA of A. niger.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"172 ","pages":"Article 103894"},"PeriodicalIF":3.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1087184524000318/pdfft?md5=8e5c0bd31a21e6eda05f460de2636e57&pid=1-s2.0-S1087184524000318-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140644490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tools to make Stachybotrys chartarum genetically amendable: Key to unlocking cryptic biosynthetic gene clusters 使纸葡萄穗霉可进行基因改造的工具：打开隐秘生物合成基因簇的钥匙

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2024-04-16 DOI: 10.1016/j.fgb.2024.103892

Katharina Steinert , Anna K. Atanasoff-Kardjalieff , Elias Messner , Markus Gorfer , Eva-Maria Niehaus , Hans-Ulrich Humpf , Lena Studt-Reinhold , Svetlana A. Kalinina

{"title":"Tools to make Stachybotrys chartarum genetically amendable: Key to unlocking cryptic biosynthetic gene clusters","authors":"Katharina Steinert , Anna K. Atanasoff-Kardjalieff , Elias Messner , Markus Gorfer , Eva-Maria Niehaus , Hans-Ulrich Humpf , Lena Studt-Reinhold , Svetlana A. Kalinina","doi":"10.1016/j.fgb.2024.103892","DOIUrl":"https://doi.org/10.1016/j.fgb.2024.103892","url":null,"abstract":"<div>The soil and indoor fungus Stachybotrys chartarum can induce respiratory disorders, collectively referred to as stachybotryotoxicosis, owing to its prolific production of diverse bioactive secondary metabolites (SMs) or mycotoxins. Although many of these toxins responsible for the harmful effects on animals and humans have been identified in the genus Stachybotrys, however a number of SMs remain elusive. Through in silico analyses, we have identified 37 polyketide synthase (PKS) genes, highlighting that the chemical profile potential of Stachybotrys is far from being fully explored. Additionally, by leveraging phylogenetic analysis of known SMs produced by non-reducing polyketide synthases (NR-PKS) in other filamentous fungi, we showed that Stachybotrys possesses a rich reservoir of untapped SMs. To unravel natural product biosynthesis in S. chartarum, genetic engineering methods are crucial. For this purpose, we have developed a reliable protocol for the genetic transformation of S. chartarum and applied it to the ScPKS14 biosynthetic gene cluster. This cluster is homologous to the already known Claviceps purpurea CpPKS8 BGC, responsible for the production of ergochromes. While no novel SMs were detected, we successfully applied genetic tools, such as the generation of deletionand overexpression strains of single cluster genes. This toolbox can now be readily employed to unravel not only this particular BGC but also other candidate BGCs present in S. chartarum, making this fungus accessible for genetic engineering.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"172 ","pages":"Article 103892"},"PeriodicalIF":3.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S108718452400029X/pdfft?md5=459b2a9a15f3da61963303e43aab77ea&pid=1-s2.0-S108718452400029X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140647790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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