Fungal Genetics and Biology最新文献

筛选
英文 中文
Identification of a regulatory factor activating the xylanase promoter of the basidiomycetous yeast Pseudozymaantarctica and its application to enhance endogenous biodegradable plastic-degrading enzyme production via transcription factor multiplexing 一种激活担子酵母南极假酶木聚糖酶启动子的调控因子的鉴定及其在通过转录因子多路复制提高内源性生物降解塑料降解酶生产中的应用。
IF 2.3 3区 生物学
Fungal Genetics and Biology Pub Date : 2026-05-01 Epub Date: 2026-05-04 DOI: 10.1016/j.fgb.2026.104084
Atsuhiro Miura , Takumi Tanaka , Hiroaki Sakai , Mizuki Tanaka , Tomotake Morita , Hiroko Kitamoto
{"title":"Identification of a regulatory factor activating the xylanase promoter of the basidiomycetous yeast Pseudozymaantarctica and its application to enhance endogenous biodegradable plastic-degrading enzyme production via transcription factor multiplexing","authors":"Atsuhiro Miura ,&nbsp;Takumi Tanaka ,&nbsp;Hiroaki Sakai ,&nbsp;Mizuki Tanaka ,&nbsp;Tomotake Morita ,&nbsp;Hiroko Kitamoto","doi":"10.1016/j.fgb.2026.104084","DOIUrl":"10.1016/j.fgb.2026.104084","url":null,"abstract":"<div><div>The basidiomycetous yeast <em>Pseudozyma antarctica</em> secretes the biodegradable plastic-degrading enzyme PaE. The xylanase promoter pPaXyn1 in <em>P. antarctica</em> is strongly induced by xylose. A <em>P. antarctica</em> reporter strain carrying the pPaXyn1 promoter-fused PaE gene (a PaE high-production cassette) produced a large amount of PaE in the presence of xylose. Accordingly, to further improve PaE productivity, this study aimed to isolate the transcription activation factor pPaXyn1. In total, 109 genes with Zn<sub>2</sub>Cys<sub>6</sub>-type DNA-binding domains were selected from the whole genome sequence of <em>P. antarctica</em> as candidate transcription factor genes. A set of strains was generated from the reporter strain in which each candidate transcription factor gene on the chromosome was disrupted. Among the disruptants, only the strain lacking the locus_tag PAN1_003r02005 (gene id: g2005) suppressed PaE production and did not show biodegradable plastic degrading activity. Moreover, a reporter strain with multiple copies of g2005 exhibited a 1.6-fold increase in PaE secretion. In addition, the strain also increased xylanase secretion. Overall, these results indicate that g2005 is a transcriptional activator of xylanase; hence, g2005 was named <em>P. antarctica</em> xylanase regulator 1 (PaXyr1). The amino acid sequence of g2005 is conserved only in the basidiomycete subphylum Smut (Ustilaginomycotina).</div></div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"184 ","pages":"Article 104084"},"PeriodicalIF":2.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147846394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A microfluidic spore chamber for long-term imaging of single-spore hyphal development. 用于单孢子菌丝发育长期成像的微流控孢子室。
IF 2.3 3区 生物学
Fungal Genetics and Biology Pub Date : 2026-05-01 Epub Date: 2026-04-27 DOI: 10.1016/j.fgb.2026.104083
Yi-Syuan Guo , Nancy P. Keller , Junko Maeda , Jean-Michel Ané , Scott T. Retterer , Tomás Allen Rush
{"title":"A microfluidic spore chamber for long-term imaging of single-spore hyphal development.","authors":"Yi-Syuan Guo ,&nbsp;Nancy P. Keller ,&nbsp;Junko Maeda ,&nbsp;Jean-Michel Ané ,&nbsp;Scott T. Retterer ,&nbsp;Tomás Allen Rush","doi":"10.1016/j.fgb.2026.104083","DOIUrl":"10.1016/j.fgb.2026.104083","url":null,"abstract":"<div><div>Understanding the life cycle of fungal spores is essential for elucidating their roles in pathogenesis, dispersal, and survival. However, studying spore development under controlled, spatially defined conditions remains challenging. Here, we present the Spore Chamber, a custom-built microfluidic platform engineered for parallel trapping and long-term imaging of individual spores under defined media conditions, enabling real-time visualization of hyphal development. Using <em>Aspergillus fumigatus</em> as a model organism, we demonstrate that sparse trapping of individual spores within size-matched trap geometries enables long-term time-lapse imaging of key developmental stages, including germination, polarized hyphal elongation, branching, and conidiophore formation. To assess the device's capacity to resolve morphogenetic responses to exogenous signals, we introduced lipochitooligosaccharides (LCOs) and short-chain chitooligosaccharides (COs). Rhizobium-derived, non-sulfated LCO (nsLCO) mixtures induced enhanced secondary branching (hyperbranching), a response not previously reported in <em>A. fumigatus</em> under these signal conditions, to our knowledge, whereas sulfated LCOs and CO4 did not significantly alter branching patterns. In addition, long-term confinement and imaging revealed rare developmental morphologies previously described primarily in mutant strains, including split conidiophore formation, elongated phialides, and stress-associated phenomena such as microcyclic conidiation, and chlamydospore development. Together, these results establish the Spore Chamber as a targeted microfluidic platform for single-spore phenotyping and long-term developmental analysis, with applications in fungal biology, chemical signaling studies, and host–microbe interaction research.</div></div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"184 ","pages":"Article 104083"},"PeriodicalIF":2.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147789571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
AI-assisted systems pharmacology with experimental validation reveal multi-target anticancer mechanisms of Ganoderma lucidum extracts against lung cancer 经实验验证的人工智能辅助系统药理学揭示了灵芝提取物抗肺癌的多靶点抗癌机制
3区 生物学
Fungal Genetics and Biology Pub Date : 2026-03-06 DOI: 10.1080/21501203.2026.2634522
Hao Wang, Leilei Chen, Siqi Zhang, Mohan Ju, Junfeng Feng, Zepei Tang, Jinwei Li, Hesham R. El‐Seedi, Yali Liu
{"title":"AI-assisted systems pharmacology with experimental validation reveal multi-target anticancer mechanisms of <i>Ganoderma lucidum</i> extracts against lung cancer","authors":"Hao Wang, Leilei Chen, Siqi Zhang, Mohan Ju, Junfeng Feng, Zepei Tang, Jinwei Li, Hesham R. El‐Seedi, Yali Liu","doi":"10.1080/21501203.2026.2634522","DOIUrl":"https://doi.org/10.1080/21501203.2026.2634522","url":null,"abstract":"The medicinal fungus Ganoderma lucidum is rich in bioactive triterpenoids and polysaccharides. The complexity and adaptive resistance of lung cancer necessitate therapeutic strategies that simultaneously disrupt multiple oncogenic pathways. We employed a systems pharmacology approach that integrates network analysis and artificial intelligence to elucidate the multitarget anticancer mechanisms of G. lucidum compounds. Bioactive metabolites were screened, and their targets were integrated with lung cancer-associated genes to construct a compound-target-pathway network. AI-based molecular docking validated key interactions. The top-predicted multitarget compounds were functionally validated in H1299 nonsmall cell lung cancer cells using viability, migration, and Western blot assays. A network of 67 synergistic targets, including PIK3CA, STAT3, EGFR, and TP53, concurrently modulates proliferation, apoptosis, and immune evasion pathways. Triterpenoids, such as lucidumol A and ganoderic acid A, act as key drivers capable of binding multiple signaling hubs. These metabolites and a standardized G. lucidum extract synergistically disrupt oncogenic signaling by inhibiting cell proliferation (extract IC50 = 1669 μg/mL), suppressing migration, and reducing STAT3 phosphorylation and c-Myc expression. These fungal metabolites exhibit potent multitarget tumor-suppressive properties.","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147381506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
WMAF: One novel method for whole mitogenome alignment in fungi and its application in phylogenetic analysis WMAF:一种新的真菌有丝分裂基因组比对方法及其在系统发育分析中的应用。
IF 2.3 3区 生物学
Fungal Genetics and Biology Pub Date : 2026-03-01 Epub Date: 2026-02-06 DOI: 10.1016/j.fgb.2026.104068
Housong Cui , Li Zhang , Tong Liu, Runmao Lin
{"title":"WMAF: One novel method for whole mitogenome alignment in fungi and its application in phylogenetic analysis","authors":"Housong Cui ,&nbsp;Li Zhang ,&nbsp;Tong Liu,&nbsp;Runmao Lin","doi":"10.1016/j.fgb.2026.104068","DOIUrl":"10.1016/j.fgb.2026.104068","url":null,"abstract":"<div><div>Fungal mitogenomes are significant for phylogenetic studies, however, constructing trees from full-length mitogenomes with varied sizes remains challenging. Recently, the dramatic increase of newly unannotated mitogenomes has created an urgent need for automated alignment tools. To address this, we developed WMAF, a novel Python-based tool that aligns full-length fungal mitogenomes by identifying and concatenating conserved genomic blocks. It overcomes recombination effects and prevents redundant locus detection. We further applied WMAF to five diverse genera, including <em>Purpureocillium</em>, <em>Fusarium</em>, <em>Saccharomyces</em>, <em>Trichoderma</em>, and <em>Rhizoctonia</em>, and generated mitochondrial trees that effectively identified intrageneric clades, as validated by nuclear trees. The method provides a foundation for advancing fungal phylogenetics and constructing the fungal tree of life.</div></div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"183 ","pages":"Article 104068"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146144688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The dynamics of transposable element content in the genome of the human pathogen Histoplasma 人致病菌组织浆体基因组中转座因子含量的动态变化。
IF 2.3 3区 生物学
Fungal Genetics and Biology Pub Date : 2026-03-01 Epub Date: 2026-01-25 DOI: 10.1016/j.fgb.2026.104057
Tania Kurbessoian , David A. Turissini , Patrick W. Kelly , Oliver Kompathoum , Jonathan A. Rader , Gaston I. Jofre , Jingbaoyi Li , McKenna Sutherland , Victoria E. Sepúlveda , Daniel R. Matute
{"title":"The dynamics of transposable element content in the genome of the human pathogen Histoplasma","authors":"Tania Kurbessoian ,&nbsp;David A. Turissini ,&nbsp;Patrick W. Kelly ,&nbsp;Oliver Kompathoum ,&nbsp;Jonathan A. Rader ,&nbsp;Gaston I. Jofre ,&nbsp;Jingbaoyi Li ,&nbsp;McKenna Sutherland ,&nbsp;Victoria E. Sepúlveda ,&nbsp;Daniel R. Matute","doi":"10.1016/j.fgb.2026.104057","DOIUrl":"10.1016/j.fgb.2026.104057","url":null,"abstract":"<div><div><em>Histoplasma</em> is a genus of human fungal pathogens that frequently affects immunosuppressed patients. Previous genetic surveys have largely focused on nucleotide-level variation, but much less attention has been given to more complex forms of mutation. Among these, transposable elements (TEs) represent an important class of mobile genetic elements that can alter genome size and play key roles in adaptation and speciation. In this study, we address this gap by examining the content and evolutionary dynamics of TEs in the human pathogen <em>Histoplasma</em>. Using previously published <em>Histoplasma</em> genome assemblies, we quantified TE content across eight phylogenetic species within the genus. Our analyses reveal heterogeneity in the evolutionary patterns of different TE families. The majority of TE orders and superfamilies show strong phylogenetic signal suggesting that phylogenetic relatedness significantly constrains the content of mobile genetic elements. We find no correlation between RNA or DNA TEs and genome size. Together, our results highlight the diverse landscape of TEs in <em>Histoplasma</em> and suggest that future studies should investigate their impact on genome evolution, fitness, and virulence.</div></div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"183 ","pages":"Article 104057"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146068528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of Atg1 in morphologic changes of the pathogenic fungus Trichosporon asahii Atg1在致病真菌朝日毛磷菌形态变化中的作用。
IF 2.3 3区 生物学
Fungal Genetics and Biology Pub Date : 2026-03-01 Epub Date: 2026-01-22 DOI: 10.1016/j.fgb.2026.104058
Mei Nakayama , Yasuhiko Matsumoto , Sanae Kurakado , Takashi Sugita
{"title":"Role of Atg1 in morphologic changes of the pathogenic fungus Trichosporon asahii","authors":"Mei Nakayama ,&nbsp;Yasuhiko Matsumoto ,&nbsp;Sanae Kurakado ,&nbsp;Takashi Sugita","doi":"10.1016/j.fgb.2026.104058","DOIUrl":"10.1016/j.fgb.2026.104058","url":null,"abstract":"<div><div><em>Trichosporon asahii</em> is a dimorphic fungus that causes severe invasive fungal infections, particularly in patients with neutropenia. Depending on nutrient availability, <em>T. asahii</em> exists in yeast, hyphae, or arthroconidia forms. Atg1, a serine/threonine kinase, involves in stress responses and virulence in several fungi. The role of Atg1 in regulating morphology, stress resistance, or virulence in <em>T. asahii</em>, however, remains poorly understood. Here, we generated three <em>atg1</em> gene-deficient <em>T. asahii</em> mutants and investigated their phenotypic characteristics to reveal the role of Atg1 in <em>T. asahii</em>. The <em>atg1</em> gene-deficient mutants exhibited no growth defects under high-temperature or various chemical stress conditions, including antifungal drugs. The mutants exhibited an increased proportion of hyphal cells when cultured in Sabouraud dextrose broth (SB), a medium commonly used for fungi. On the other hand, no morphologic differences were observed between the parent strain and the <em>atg1</em> gene-deficient mutants under a nitrogen-limited condition. The virulence of these <em>atg1</em> gene-deficient mutants was maintained in a silkworm infection model. Furthermore, all three generated <em>atg1</em> gene-deficient mutants exhibited consistent phenotypes. Our findings suggest that while Atg1 does not play a major role in stress tolerance or virulence in <em>T. asahii</em> under the tested conditions, it plays a role in regulating its dimorphic morphologic changes.</div></div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"183 ","pages":"Article 104058"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146044474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hyphae: Tubular creators of the fungal kingdom 菌丝:真菌王国的管状创造者。
IF 2.3 3区 生物学
Fungal Genetics and Biology Pub Date : 2026-02-01 Epub Date: 2025-12-10 DOI: 10.1016/j.fgb.2025.104054
Salomon Bartnicki-García, Meritxell Riquelme
{"title":"Hyphae: Tubular creators of the fungal kingdom","authors":"Salomon Bartnicki-García,&nbsp;Meritxell Riquelme","doi":"10.1016/j.fgb.2025.104054","DOIUrl":"10.1016/j.fgb.2025.104054","url":null,"abstract":"","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"182 ","pages":"Article 104054"},"PeriodicalIF":2.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145745785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
AnBir1: a conserved fungal inhibitor of apoptosis regulates caspase activity and core processes in Aspergillus nidulans AnBir1:一种保守的真菌细胞凋亡抑制剂,调控半胱天冬酶活性和核心过程。
IF 2.3 3区 生物学
Fungal Genetics and Biology Pub Date : 2026-02-01 Epub Date: 2025-11-25 DOI: 10.1016/j.fgb.2025.104047
Meareg G. Amare , Miette K. Hennessy , Grant Nickles , Sung Chul Park , Sachin Jain , Neta Shlezinger , Nancy P. Keller , Mehdi Kabbage
{"title":"AnBir1: a conserved fungal inhibitor of apoptosis regulates caspase activity and core processes in Aspergillus nidulans","authors":"Meareg G. Amare ,&nbsp;Miette K. Hennessy ,&nbsp;Grant Nickles ,&nbsp;Sung Chul Park ,&nbsp;Sachin Jain ,&nbsp;Neta Shlezinger ,&nbsp;Nancy P. Keller ,&nbsp;Mehdi Kabbage","doi":"10.1016/j.fgb.2025.104047","DOIUrl":"10.1016/j.fgb.2025.104047","url":null,"abstract":"<div><div>Regulated cell death (RCD) is a highly conserved and coordinated cellular demise process. In animals, the fate of cells depends on the precise regulation of RCD, with inhibitors of apoptosis proteins (IAPs) playing a major role as negative regulators of cellular death. In fungi, RCD regulates crucial processes, including growth, development, stress response, host-pathogen interactions and more. However, the biochemical details of this process are not well understood in this kingdom. IAPs are, remarkably, one of the few RCD/Apoptosis-regulatory proteins that are conserved in fungi. Here, we performed large-scale bioinformatic analyses of IAPs in sequenced fungal genomes. While most fungal organisms have a single IAP gene (81.65 % of the genomes analyzed here), some fungi lack IAPs altogether and others have multiple IAP-like genes. Using the <em>Aspergillus nidulans</em> IAP, AnBir1, we show that this protein is required for survival and regulates cell death by inhibiting caspase-like activity. Moreover, disrupting RCD by constitutively expressing AnBir1 impacted fundamental processes, including development, stress response and secondary metabolism. We also show that fungal RCD can be hijacked for therapeutic purposes. Using virus-induced gene silencing (VIGS), we targeted the AnBir1 homolog of the plant pathogenic fungus <em>Sclerotinia sclerotiorum</em> (SsBir1) during plant infection. Targeting SsBir1 resulted in enhanced resistance to <em>S. sclerotiorum</em> infection. We propose that IAPs play a critical role in regulating caspase activities and other RCD-related processes in fungi and may constitute a novel therapeutic target for fungal infections.</div></div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"182 ","pages":"Article 104047"},"PeriodicalIF":2.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145642839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fungi from Brazilian Oceanic Islands: Identification and genome characterization of Periconia belmontensis sp. nov. isolated from São Pedro and São Paulo archipelago 来自巴西大洋岛屿的真菌:从<s:1> o Pedro和<e:1> o Paulo群岛分离的Periconia belmontensis sp. 11 .的鉴定和基因组特征。
IF 2.3 3区 生物学
Fungal Genetics and Biology Pub Date : 2026-02-01 Epub Date: 2025-12-11 DOI: 10.1016/j.fgb.2025.104055
Victoria Pommer , Renan do Nascimento Barbosa , Felipe Geremia , Igor Daniel Alves , Matheus da Silva Camargo , Esther Maria Rocha , Nicolau Sbaraini , Charley Staats , Cristina Maria de Souza-Motta , Augusto Schrank , Marilene Henning Vainstein
{"title":"Fungi from Brazilian Oceanic Islands: Identification and genome characterization of Periconia belmontensis sp. nov. isolated from São Pedro and São Paulo archipelago","authors":"Victoria Pommer ,&nbsp;Renan do Nascimento Barbosa ,&nbsp;Felipe Geremia ,&nbsp;Igor Daniel Alves ,&nbsp;Matheus da Silva Camargo ,&nbsp;Esther Maria Rocha ,&nbsp;Nicolau Sbaraini ,&nbsp;Charley Staats ,&nbsp;Cristina Maria de Souza-Motta ,&nbsp;Augusto Schrank ,&nbsp;Marilene Henning Vainstein","doi":"10.1016/j.fgb.2025.104055","DOIUrl":"10.1016/j.fgb.2025.104055","url":null,"abstract":"<div><div>The genus <em>Periconia</em> is recognized for its ability to produce several organic compounds with biotechnological potential. Despite the importance of these fungi, genomic studies on <em>Periconia</em> species remain limited. In this study, we performed a morphological and whole-genome sequence characterization of <em>Periconia belmontensis</em> sp. nov. isolated from the São Pedro and São Paulo Archipelago, Brazilian oceanic islets at the Mid-Atlantic Ridge. Our results indicate that potato dextrose agar and malt extract agar are optimal for fungal growth at 28 °C. Multilocus phylogenetic analysis and morphological characters support the establishment of the new species. Genome sequence assembly revealed a genome size of 50 Mb, whereas secondary metabolite analysis identified fifty putative biosynthetic gene clusters (BGCs). Gene conservation analysis revealed 1856 unique genes, mainly related to capturing and processing nutrient activity, compared with four others available <em>Periconia</em> genomes. The present study contributes to the expansion of molecular databases for <em>Periconia</em>. These findings also assist in silico exploration of novel molecules and increase the understanding of the biotechnological potential of these species.</div></div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"182 ","pages":"Article 104055"},"PeriodicalIF":2.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145745834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Life cycle transcriptomics of the homothallic cultivated morel (Morchella rufobrunnea) 同源栽培羊肚菌(羊肚菌)的生命周期转录组学。
IF 2.3 3区 生物学
Fungal Genetics and Biology Pub Date : 2026-02-01 Epub Date: 2025-12-18 DOI: 10.1016/j.fgb.2025.104056
Judson Van Wyk , Xinxin Wang , Davis Mathieu , Gary L. Mills , Kevin L. Childs , Gregory Bonito
{"title":"Life cycle transcriptomics of the homothallic cultivated morel (Morchella rufobrunnea)","authors":"Judson Van Wyk ,&nbsp;Xinxin Wang ,&nbsp;Davis Mathieu ,&nbsp;Gary L. Mills ,&nbsp;Kevin L. Childs ,&nbsp;Gregory Bonito","doi":"10.1016/j.fgb.2025.104056","DOIUrl":"10.1016/j.fgb.2025.104056","url":null,"abstract":"<div><div>Morels (<em>Morchella</em> spp.) are iconic edible spring mushrooms renowned for their unique, meaty and earthy flavor profile. Wild-foraged morels are heavily relied upon to meet consumer demands, but optimizing cultivation is becoming critical as popularity continues to grow. The mating systems of <em>Morchella</em> spp. are of particular interest given their relevance to cultivation. Both heterothallic (outcrossing) and homothallic (self-fertile) life cycles have been reported. Here, we sequenced the genome of a commercial strain of <em>Morchella rufobrunnea</em> (GMI-8) to examine the genomic architecture surrounding the mating-type (MAT) loci and to provide a reference for transcriptomic analyses across its life cycle. To investigate gene regulation across developmental stages, we generated transcriptomes from six tissue types (sclerotia, mycelium, conidia, fundament, stipe, and hymenium) representing five key life stages. This sampling allowed us to profile the expression of mating-related, growth, and developmental associated genes across these life stages. Our analyses revealed a haploid genome size of 58 Mb, and co-localization of both MAT genes as required for sexual reproduction, confirming homothallism in this species. MAT genes (MAT1–1-1, 1–1-10, and 1–2-1) were highly expressed in conidial, fundament, and hymenial tissues, supporting the hypothesis that conidia may function as a nuclear donor/spermatia. This work improves our understanding of sexual reproduction and the life cycle of <em>Morchella,</em> and establishes a genomic and transcriptomic foundation for future studies of <em>Morchella</em> developmental biology.</div></div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"182 ","pages":"Article 104056"},"PeriodicalIF":2.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145800862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信
小红书