Infection Genetics and Evolution最新文献_第7页

Comparative structural insights of X protein across species and the “lost” BH3-like domain that may explain the absence of hepatocellular carcinoma in birds 跨物种X蛋白的比较结构见解和“丢失的”bh3样结构域可能解释鸟类肝细胞癌的缺失

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-06-03 DOI: 10.1016/j.meegid.2025.105777

Kuldeep Kaur , Justine Beghin , Vanessa Meier-Stephenson

{"title":"Comparative structural insights of X protein across species and the “lost” BH3-like domain that may explain the absence of hepatocellular carcinoma in birds","authors":"Kuldeep Kaur , Justine Beghin , Vanessa Meier-Stephenson","doi":"10.1016/j.meegid.2025.105777","DOIUrl":"10.1016/j.meegid.2025.105777","url":null,"abstract":"<div><div>Chronic hepatitis B virus (HBV) infection is a leading cause of hepatocellular carcinoma (HCC). HBV produces an X protein (HBx) that is heavily linked to HCC, a similar finding seen across the <em>Orthohepadnaviruses.</em> There is an analogous, but truncated form of the X protein in <em>Avihepadnaviruses</em>, but no HCC is seen in infected avian species. This raises questions about the differences between mammalian and avian X proteins and their potential roles in carcinogenesis. Here we explore this question from a structural perspective of X proteins across mammalian and avian hepadnaviruses to determine whether there are any structural features linking these interesting observations. We first compile sequences to create consensus sequences with which to align with each other and subsequently to input into modeling programs, RoseTTAFold (RF) and AlphaFold3 (AF3). Comparative analyses show that mammalian X proteins are longer (154aa and 141aa, respectively for HBx and WHx) and have distinct domains in their C-terminal region, including a BH3-like domain that has been linked with many cancer pathways. Avian X proteins, however, are truncated with a similarly located stop codon. This truncation rids the protein of the BH3-like domain. We sought to find this domain by theoretical read-through or frameshifts and indeed, an analogous BH3-like domain is found that can similarly bind the BH3 target Bcl-2. Based on this work, it may be evolutionarily plausible that a single-nucleotide insertion led to the stop codon and BH3-domain loss that may account for the lack of cancers seen with <em>Avihepadnaviruses</em>.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"132 ","pages":"Article 105777"},"PeriodicalIF":2.6,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic characterization of hepaciviruses and pegivirus in the northern tree shrew (Tupaia belangeri) 北方树鼩（Tupaia belangeri）肝炎病毒和pegivirus的基因组特征。

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-06-02 DOI: 10.1016/j.meegid.2025.105778

Takahiro Sanada , Kyoko Tsukiyama-Kohara , Michinori Kohara

{"title":"Genomic characterization of hepaciviruses and pegivirus in the northern tree shrew (Tupaia belangeri)","authors":"Takahiro Sanada , Kyoko Tsukiyama-Kohara , Michinori Kohara","doi":"10.1016/j.meegid.2025.105778","DOIUrl":"10.1016/j.meegid.2025.105778","url":null,"abstract":"<div><div>The northern tree shrew (<em>Tupaia belangeri</em>) is recognized as a valuable animal model for studying hepatitis B and C viruses (HCV). However, natural infections of tree shrews remain to be fully characterized. In this study, we identified tree shrew hepaciviruses 1 and 2 and tree shrew pegivirus in liver samples and determined their complete genome sequences. The sequences of these viruses encoded a single large polyprotein, similar to other <em>Flaviviridae</em> viruses. In the 5′ untranslated region, tree shrew hepaciviruses possesses miR-122 binding sites, which is involved in liver tropism of HCV, whereas tree shrew pegivirus did not. Genetic analysis revealed 58 % amino acid homology between the polyproteins of tree shrew hepaciviruses 1 and 2. Tree shrew hepacivirsues were genetically close to the rodent hepacivirus (<em>Hepacivirus P</em>) detected in long-tailed ground squirrels, and tree shrew pegivirus was close to <em>Pegivirus scotophili</em> detected in bats. Analysis of viral infections revealed that, among the 37 tree shrews tested, 26 were positive for tree shrew hepacivirus 1, of which 15 were also positive for tree shrew hepacivirus 2. Only one tree shrew was positive for the pegivirus. These data indicate that the tree shrews used as experimental animals were infected with various viruses and that viral genomic characterizations are essential for classifying viral species and understanding viral characteristics and evolution. Furthermore, this study will be useful for screening viral infections to establish the tree shrew as a more stable experimental animal.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"132 ","pages":"Article 105778"},"PeriodicalIF":2.6,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling the genomes of multidrug-resistant Klebsiella quasipneumoniae: A study of clinical and environmental isolates 多重耐药准肺炎克雷伯菌基因组的揭示：临床和环境分离株的研究

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-06-02 DOI: 10.1016/j.meegid.2025.105773

Berfin Eroğlu , Eda Delik , Burcu Emine Tefon-Öztürk

{"title":"Unravelling the genomes of multidrug-resistant Klebsiella quasipneumoniae: A study of clinical and environmental isolates","authors":"Berfin Eroğlu , Eda Delik , Burcu Emine Tefon-Öztürk","doi":"10.1016/j.meegid.2025.105773","DOIUrl":"10.1016/j.meegid.2025.105773","url":null,"abstract":"<div><div><em>Klebsiella quasipneumoniae</em> is an opportunistic pathogen that predominantly resides in the human gut posing a significant risk of severe infections in individuals with compromised immune systems. In this study, whole genome sequencing of two multi-drug-resistant clinical and freshwater <em>K. quasipneumoniae</em> subsp. <em>similipneumoniae</em> isolates was performed and compared using Illumina sequencing technology. The genome size of the clinical isolate and freshwater isolate was 5.23 Mbp and 5.22 Mbp, respectively, with a Guanine-cytosine (GC) content of 57.77 % and 57.21 %. Genomic analyses identified 29 genes associated with antimicrobial resistance, mainly related to efflux pumps. CRISPR sequences were also predicted, of which 4 were identified in the freshwater isolate and 1 in the clinical isolate. Genomic islets (GIts) and genomic islands (GIs) were also delineated using IslandViewer4. The freshwater isolate contained 13 GIs and 16 GIts, while the clinical isolate contained 14 GIs and 19 GIts, harbouring important virulence and antimicrobial resistance genes (such as <em>acr</em>, <em>bdcA, fim</em> and <em>norB</em>). PHASTEST analysis revealed six intact phage regions in the freshwater isolate and five in the clinical isolate. Finally, a Maximum Likelihood tree was constructed based on the amino acid sequences of 440 orthologous genes from the 98 <em>K. quasipneumoniae</em> genomes, showing that the isolates were positioned within distinct internal clades.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"132 ","pages":"Article 105773"},"PeriodicalIF":2.6,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144220851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reverse genetics system for Tick-borne encephalitis virus using Circular Polymerase Extension Reaction 利用循环聚合酶延伸反应的蜱传脑炎病毒反向遗传系统

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-06-01 DOI: 10.1016/j.meegid.2025.105776

Saki Mitsunaga , Tomokazu Tamura , Samuel Nyampong , Takasuke Fukuhara , Hiroshi Shimoda , Daisuke Hayasaka

{"title":"Reverse genetics system for Tick-borne encephalitis virus using Circular Polymerase Extension Reaction","authors":"Saki Mitsunaga , Tomokazu Tamura , Samuel Nyampong , Takasuke Fukuhara , Hiroshi Shimoda , Daisuke Hayasaka","doi":"10.1016/j.meegid.2025.105776","DOIUrl":"10.1016/j.meegid.2025.105776","url":null,"abstract":"<div><div>Tick-borne encephalitis virus (TBEV) belongs to the Family <em>Flaviviridae</em>, Genus <em>Orthoflavivirus</em>, and causes severe neurological diseases in humans. The reverse genetics system is a basic tool for viral research; however, cloning the genome of orthoflavivirus using bacterial plasmids is difficult because of their toxicity to <em>Escherichia coli.</em> Polymerase chain reaction-based Circular Polymerase Extension Reaction (CPER), an <em>E. coli</em>-free reverse genetics system, has been recently applied to several RNA viruses. However, there have been no reports on recombinant TBEV produced using CPER. In this study, we attempted to produce recombinant TBEV Oshima, Sofjin and Hypr strains by CPER. Genome sequencing and plaque-forming assays were performed to determine the properties of rescued TBEVs. In addition, infectivity and pathogenicity of rescued TBEVs was also monitored in C57BL/6 mice. Rescued TBEVs of Oshima, Sofjin and Hypr caused apparent cytopathic effect and efficient propagations in BHK cells, and showed intrinsic virulence in mice. The rescued TBEVs showed several nucleotide and amino acid substitutions compared to the original viral sequences. These results showed that infectious TBEVs from the Oshima, Sofjin, and Hypr strains were produced using CPER. We propose that future applications of this method will contribute to related research on TBEV.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"132 ","pages":"Article 105776"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic analysis of peste des petits ruminants virus in Europe: Common origin for emergence in Greece, Romania, and Bulgaria 欧洲小反刍兽疫病毒的基因组分析：希腊、罗马尼亚和保加利亚出现的共同起源

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-05-29 DOI: 10.1016/j.meegid.2025.105774

Samia Guendouz , Olivier Kwiatek , Aikaterini Kirtzalidou , Angeliki Katsifa , Maria Gianniou , Corina Ancuceanu , Mona Ghiță , Cristian Laurențiu Mortasivu , Anna Zdravkova , Iliyan Kostov , Emilia Ivanova , Florica Bărbuceanu , Konstantia E. Tasioudi , Arnaud Bataille

{"title":"Genomic analysis of peste des petits ruminants virus in Europe: Common origin for emergence in Greece, Romania, and Bulgaria","authors":"Samia Guendouz , Olivier Kwiatek , Aikaterini Kirtzalidou , Angeliki Katsifa , Maria Gianniou , Corina Ancuceanu , Mona Ghiță , Cristian Laurențiu Mortasivu , Anna Zdravkova , Iliyan Kostov , Emilia Ivanova , Florica Bărbuceanu , Konstantia E. Tasioudi , Arnaud Bataille","doi":"10.1016/j.meegid.2025.105774","DOIUrl":"10.1016/j.meegid.2025.105774","url":null,"abstract":"<div><div>Outbreaks of the highly pathogenic small ruminant disease peste des petits ruminants (PPR) were reported in Greece and Romania in July 2024, and central Bulgaria in November 2024. The origin and the link between these outbreaks are not clear. In this study, genome sequences of PPR virus were obtained from samples collected by veterinary authorities in the first farms notified as infected in the three European countries. Genomic analyses confirmed that the emergence of PPR across Europe has a common origin, pointing towards an introduction from Northern Africa, although additional sequencing from the virus currently circulating globally is needed to confirm this hypothesis. More sequencing from the different outbreaks in Europe could also help to resolve the pathway of PPR transmission between and within European countries. Multiple nucleotide and amino acid differences separate the genomes from Europe from other sequences, with potential impact on the functionality of viral proteins to be investigated.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"132 ","pages":"Article 105774"},"PeriodicalIF":2.6,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic evolution of respiratory syncytial virus in pediatric acute respiratory infections: Insights from a non-epidemic season 小儿急性呼吸道感染呼吸道合胞病毒的遗传进化：来自非流行季节的见解

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-05-28 DOI: 10.1016/j.meegid.2025.105775

Xiujie Gao , Rong Zou , Weiqin Zhou , Hongxia Zhao , Cuiling Lu , Jia Tian , Jindou Hao , Powei Tang , Kai Wu , Yu Zhang , Xiang Yuan , Chao Yang , Peihui Liu

{"title":"Genetic evolution of respiratory syncytial virus in pediatric acute respiratory infections: Insights from a non-epidemic season","authors":"Xiujie Gao , Rong Zou , Weiqin Zhou , Hongxia Zhao , Cuiling Lu , Jia Tian , Jindou Hao , Powei Tang , Kai Wu , Yu Zhang , Xiang Yuan , Chao Yang , Peihui Liu","doi":"10.1016/j.meegid.2025.105775","DOIUrl":"10.1016/j.meegid.2025.105775","url":null,"abstract":"<div><h3>Background</h3><div>Respiratory syncytial virus (RSV) is an important cause of acute respiratory infections (ARI) in children, with recent outbreaks increasing the global burden. The 2021 season saw a significant increase in cases from June to August, possibly due to COVID-19 control measures. The RSV-G region contributes to significant strain variation that can lead to severe epidemics. Understanding the impact of RSV genetic diversity on epidemics and disease severity is critical for prevention and management. This study aimed to investigate the genomic characteristics of RSV-G and provide insights for vaccine development and RSV control.</div></div><div><h3>Methods</h3><div>Seventy-four hospitalized ARI cases were identified from May to July 2021. Nasopharyngeal swab samples were collected for RSV-A and RSV-B detection by RT-qPCR. G-gene amplification, sequencing, and alignment were performed for genetic analysis. Phylogenetic trees were constructed, and whole genome sequencing was performed.</div></div><div><h3>Results</h3><div>Of the 74 cases, 35.1 % were infants under 1 year old, and 6.76 % were severe RSV infections. RSVA accounted for 56.76 % of cases, while RSVB accounted for 43.24 %. Unique mutations were observed, possibly related to epidemic trends and disease severity. Phylogenetic analysis revealed significant differences from reference strains, particularly in a unique combination of three amino acid sites in the RSV-A G protein associated with disease severity.</div></div><div><h3>Conclusions</h3><div>This study provides insights into the genetic diversity of RSV during the 2021 off-season epidemic in Shenzhen and reveals the emergence of a new phylogenetic sublineage. Understanding the genetic diversity of RSV is critical for developing effective vaccines and controlling RSV outbreaks.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"132 ","pages":"Article 105775"},"PeriodicalIF":2.6,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simulating epidemic peak dynamics on complex networks using efficient Gillespie algorithms 利用高效Gillespie算法模拟复杂网络上的疫情峰值动态

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-05-27 DOI: 10.1016/j.meegid.2025.105768

Yulian Kuryliak , Michael T.M. Emmerich , Dmytro Dosyn

{"title":"Simulating epidemic peak dynamics on complex networks using efficient Gillespie algorithms","authors":"Yulian Kuryliak , Michael T.M. Emmerich , Dmytro Dosyn","doi":"10.1016/j.meegid.2025.105768","DOIUrl":"10.1016/j.meegid.2025.105768","url":null,"abstract":"<div><div>We present an integrated study of epidemic spreading on complex networks that (i) reveals how network structure and targeted interventions shape the peak count of infected nodes (PCIN) and its timing, (ii) supplies an open-source dashboard that lets researchers explore these effects with realistic model extensions, and (iii) delivers a high-performance simulation engine which improves the time complexity of existing sparse network implementations of Gillespie's algorithm by multiplicative factors. Continuous-time SI/SIS/SIR dynamics are analyzed with respect to two intervention knobs: edge-specific infection rate reduction and node-level recovery acceleration, yielding explicit bounds on peak height and delay across heterogeneous topologies. To test scenarios interactively, we extend the dashboard simulator to include non-exponential recovery times, temporal rewiring, weighted and multi-type contacts, simulation of antigenically equivalent mutant strains, and a novel visual aggregation of likely infection routes. Moreover, we redesign Gillespie's algorithm for sparse graphs at its core by succinctly and incrementally updating the (sums of the) transition rate and maintaining a sorted infected node list, achieving speed increases with a multiplicative factor compared to the state-of-the-art implementation of the adjacency list. Additional speed gains can be achieved by our new algorithm when infection is in its early stage and only a few nodes of a large network are infected; a complementary dense matrix variant covers non-sparse cases. Benchmarks on Barabási–Albert networks confirm up-to-order-of-magnitude gains over the standard adjacency-list implementation of Gillespie's algorithm.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"132 ","pages":"Article 105768"},"PeriodicalIF":2.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular epidemiology of West Nile Virus in raptors, Connecticut, USA, 2022: A case series with whole genome sequencing and phylogenetic analysis 西尼罗病毒在美国康涅狄格州猛禽的分子流行病学：全基因组测序和系统发育分析的病例系列。

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-05-25 DOI: 10.1016/j.meegid.2025.105769

Zeinab H. Helal , Nina Francesca Soriano , David H. Chung , Dong-Hun Lee , Natalie Tocco , Fatma Gazeyoglu , Ji-Yeon Hyeon , Guillermo R. Risatti

{"title":"Molecular epidemiology of West Nile Virus in raptors, Connecticut, USA, 2022: A case series with whole genome sequencing and phylogenetic analysis","authors":"Zeinab H. Helal , Nina Francesca Soriano , David H. Chung , Dong-Hun Lee , Natalie Tocco , Fatma Gazeyoglu , Ji-Yeon Hyeon , Guillermo R. Risatti","doi":"10.1016/j.meegid.2025.105769","DOIUrl":"10.1016/j.meegid.2025.105769","url":null,"abstract":"<div><div>West Nile virus (WNV), a mosquito-borne flavivirus, circulates in an enzootic cycle between birds and mosquitoes, with raptors serving as key amplifying hosts. Despite their importance in WNV surveillance, complete genome sequences from raptors remain limited. This study aimed to investigate the genetic diversity and evolutionary history of WNV in raptors from Connecticut, USA. Samples were collected in 2022 from the brain tissue of deceased red-tailed hawks, red-shouldered hawks, Cooper's hawks, a peregrine falcon, and American crows. Complete protein-coding sequences (CDSs) of 19 WNV isolates were obtained using multiplex tiling reverse transcription polymerase chain reaction (RT-PCR) developed in this study and Illumina iSeq100 sequencing. Phylogenetic analyses revealed our sequences were grouped into two monophyletic clusters and two singletons within lineage 1, showing genetic similarities to WNV strains detected in mosquitoes in New York (2012–2015) rather than earlier strains from Connecticut (1999–2008). Bayesian analysis indicated at least four independent introductions, with the estimated time to the most recent common ancestor (tMRCA) for Clusters 1 and 2 in April 2009 and February 2010, respectively. The mean substitution rate was 4.30 × 10<sup>−4</sup> substitutions/site/year. All sequences contained the T249P mutation in NS3, which has been linked to reduced virulence in avian models. These findings provide valuable reference data for future WNV genomic surveillance studies, emphasizing the role of raptors as sentinel species and the need for ongoing genomic surveillance to monitor WNV evolution, transmission, and potential public health risks.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"132 ","pages":"Article 105769"},"PeriodicalIF":2.6,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Foot-and-mouth disease virus O/ME-SA/SA-2018: A new emerging threat posed by viruses circulating in Asia? 口蹄疫病毒O/ME-SA/SA-2018：亚洲流行的病毒构成的新威胁？

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-05-24 DOI: 10.1016/j.meegid.2025.105771

Natasha Edwards , Andrew E. Shaw , Antonello Di Nardo , Amy Sowood , Hayley M. Hicks , Jemma Wadsworth , Krupali Parekh , Amy Mccarron , Pradeep Lakpriya Kumarawadu , Yassir Mohammed Eltahir , Meera Saeed Mohamed , Lekh Raj Dahal , Krishna Raj Pandey , Nisha Poudel , Emma Taylor , Daniel L. Horton , Valerie Mioulet , Anna Ludi , Nick J. Knowles , Donald P. King , Lidia Lasecka-Dykes

{"title":"Foot-and-mouth disease virus O/ME-SA/SA-2018: A new emerging threat posed by viruses circulating in Asia?","authors":"Natasha Edwards , Andrew E. Shaw , Antonello Di Nardo , Amy Sowood , Hayley M. Hicks , Jemma Wadsworth , Krupali Parekh , Amy Mccarron , Pradeep Lakpriya Kumarawadu , Yassir Mohammed Eltahir , Meera Saeed Mohamed , Lekh Raj Dahal , Krishna Raj Pandey , Nisha Poudel , Emma Taylor , Daniel L. Horton , Valerie Mioulet , Anna Ludi , Nick J. Knowles , Donald P. King , Lidia Lasecka-Dykes","doi":"10.1016/j.meegid.2025.105771","DOIUrl":"10.1016/j.meegid.2025.105771","url":null,"abstract":"<div><div>In 2018, a novel lineage of foot-and-mouth disease virus (FMDV) termed O/ME-SA/SA-2018 was identified in India, which subsequently spread into neighbouring countries (Nepal, Bangladesh and Sri Lanka). Since then, incursions of this lineage have occurred in the Middle East, beyond its usual epidemiological distribution. Using sequence data derived from field cases, this paper reconstructs the evolutionary history of the O/ME-SA/SA-2018 lineage, presents vaccine matching data for six commercially available FMD vaccines, and describes a novel lineage-specific RT-PCR assay which can be used to help detect field cases due this emerging lineage. Further surveillance studies are warranted to understand the distribution of this virus in Asian countries.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"132 ","pages":"Article 105771"},"PeriodicalIF":2.6,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three distinct forms of Pneumocystis coexist in individuals of two species of deer mice (genus Peromyscus) 三种不同形式的肺囊虫共存于两种鹿鼠（鹿鼠属）的个体。

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-05-21 DOI: 10.1016/j.meegid.2025.105767

Spenser J. Babb-Biernacki , Li Peng , Claire M. Jardine , Jamie L. Rothenburger , Mark T. Swanson , Joseph A. Kovacs , Jacob A. Esselstyn , Vinson P. Doyle , Liang Ma

{"title":"Three distinct forms of Pneumocystis coexist in individuals of two species of deer mice (genus Peromyscus)","authors":"Spenser J. Babb-Biernacki , Li Peng , Claire M. Jardine , Jamie L. Rothenburger , Mark T. Swanson , Joseph A. Kovacs , Jacob A. Esselstyn , Vinson P. Doyle , Liang Ma","doi":"10.1016/j.meegid.2025.105767","DOIUrl":"10.1016/j.meegid.2025.105767","url":null,"abstract":"<div><div>As emerging zoonoses represent a significant public health threat, understanding how pathogens' host ranges evolve is critical to protect human and wildlife health. Closely related hosts infected with host-specific pathogens provide valuable opportunities for clear inferences of host range evolution, as they allow for the examination of early diversification patterns in their resident pathogens. <em>Pneumocystis</em>, an obligate lung symbiont that is believed to be ubiquitous in mammals, exemplifies such a model. To explore the early stages of divergence in <em>Pneumocystis,</em> we collected geographically dispersed samples from two sister species of deer mice: <em>Peromyscus leucopus</em> (white-footed mice) and <em>Peromyscus gossypinus</em> (cotton mice<em>).</em> We sequenced two nuclear and two mitochondrial loci of <em>Pneumocystis</em> sampled from the lungs of these mice. These sequences revealed three distinct <em>Pneumocystis</em> taxa, two of which were found to cross-infect both host species and were often found coexisting within the same individual. Genetic diversity and phylogenetic analysis suggest that the three <em>Pneumocystis</em> taxa represent separate species. Further analysis of the mitochondrial large subunit rRNA gene from the most common taxon of these three revealed that host geographic origins influenced <em>Pneumocystis</em> genetic structure more than host species identity. Nevertheless, the results also suggest an overall interconnectedness of the symbiont metapopulation.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"132 ","pages":"Article 105767"},"PeriodicalIF":2.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144133194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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