Infection Genetics and Evolution最新文献

{"title":"Molecular detection of Anaplasma phagocytophilum and Rickettsia monacensis in trombiculid mite pools collected from wild rodents in Korea: Implications for potential mite-borne transmission.","authors":"Dong-Jae Yu, Dong-Min Kim, Choon-Mee Kim, Hyeon Je Song, Jeong-Chi Lee","doi":"10.1016/j.meegid.2025.105789","DOIUrl":"https://doi.org/10.1016/j.meegid.2025.105789","url":null,"abstract":"<p><p>Mite-borne diseases have been extensively studied; however, limited research has focused on the detection of Rickettsia species other than Orientia tsutsugamushi in Korean mites. Polymerase chain reaction (PCR) assays were performed using mites collected from rodents. Nested PCR (N-PCR) targeting the 56 kDa gene of O. tsutsugamushi showed a 0.29 % positivity rate (11/3855) and a 0.1 % minimum infection rate (MIR). The sca1 gene-targeted N-PCR for Rickettsia showed a 1.27 % positivity rate (49/3855) and a 0.005 % MIR, revealing the presence of Rickettsia monacensis. For Anaplasma phagocytophilum detection, N-PCR targeting ankA gene showed a 4.31 % positivity rate (166/3855) and a 0.16 % MIR, while N-PCR targeting the 16S rRNA gene showed a 0.78 % positivity rate (30/3855) and a 0.03 % MIR. Our findings confirm the presence of A. phagocytophilum, R. monacensis, and O. tsutsugamushi DNA in trombiculid mites collected from wild rodents in Korea. Although these pathogens are recognized as human disease agents, the detection of DNA alone does not provide evidence of vector competence. Consequently, further experimental studies are warranted to clarify the potential role of trombiculid mites in the transmission cycles of A. phagocytophilum and R. monacensis. KEYPOINTS: Anaplasma phagocytophilum and Rickettsia monacensis were detected in trombiculid mites collected from wild rodents captured in Korea, suggesting the need for further research on the role of parasitic mites in the human transmission of A. phagocytophilum and R. monacensis.</p>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":" ","pages":"105789"},"PeriodicalIF":2.6,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance profiles of gram-negative bacteria in southern Tunisia: Focus on ESBL, carbapenem and colistin resistance","authors":"Mariem Yengui ,&nbsp;Rahma Trabelsi ,&nbsp;Radhouane Gdoura ,&nbsp;Aïcha Hamieh ,&nbsp;Hanane Zerrouki ,&nbsp;Jean-Marc Rolain ,&nbsp;Linda Hadjadj","doi":"10.1016/j.meegid.2025.105787","DOIUrl":"10.1016/j.meegid.2025.105787","url":null,"abstract":"<div><div>The main objective of this cross-sectional study was to investigate the prevalence of beta-lactam (cephalosporins or carbapenems) or colistin resistant bacteria. Those were isolated from urine samples in two private polyclinics located in the Sfax region, in southern Tunisia. From September 2021 to August 2022, 116 strains resistant to β-lactams or colistin were isolated, identified by MALDI-TOF, and their antibiotic susceptibility was assessed by disk diffusion method. Resistance genes were detected by real-time PCR, standard PCR, and sequencing. The results revealed that the 116 strains consisted predominantly of Enterobacteriaceae (92.2 %) and non-fermenting bacteria (7.8 %). Among these strains, 21 (18.1 %) were resistant to carbapenems, three (2.7 %) to colistin, including two strains of <em>Klebsiella pneumoniae</em> (1.7 %) exhibiting resistance to both carbapenems and colistin. In <em>Enterobacteriaceae, bla</em>_CTX-A, <em>bla</em>_SHV, and <em>bla</em>_TEM were found in 79.5 %, 46.7 %, and 40.2 % of strains, respectively. For these strains, the minimum inhibitory concentrations (MICs) of imipenem and ertapenem ranged from &gt;32 to 6 μg/mL and &gt; 32 to 2 μg/mL, respectively, with <em>bla</em>_OXA-48 and <em>bla</em>_NDM detected in 21.7 % and 19.6 % of isolates, respectively. Seven <em>A. baumannii</em> isolates resistant to imipenem and meropenem (MICs &gt;32 μg/mL and 8 μg/mL, respectively) carried <em>bla</em>_OXA-23 (<em>n</em> = 5) and <em>bla</em>_OXA-24 (<em>n</em> = 2). In addition, mutations in the <em>mgrB</em> gene conferring colistin resistance were identified in two isolates. Two <em>K. pneumoniae</em> were colistin-resistant and carried the <em>bla</em>_OXA-48 gene. These results highlight the urgency of developing new strategies for the identification and surveillance of pathogenic strains in humans to effectively combat this growing public health threat in Tunisia.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"133 ","pages":"Article 105787"},"PeriodicalIF":2.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracing the environmental source of fatal community-acquired pneumonia and sepsis caused by Burkholderia pseudomallei","authors":"Revathy Arushothy ,&nbsp;Norazlah Bahari ,&nbsp;Punitha Manoharan ,&nbsp;Mohammad Ridhuan Mohd Ali ,&nbsp;Siti Nur Zawani Rosli ,&nbsp;Nazirah Samsuddin ,&nbsp;Nur Sulhi Ilani Che Unik ,&nbsp;Fairuz Amran ,&nbsp;Ratna Mohd Tap ,&nbsp;Rohaidah Hashim ,&nbsp;Sheila Nathan","doi":"10.1016/j.meegid.2025.105788","DOIUrl":"10.1016/j.meegid.2025.105788","url":null,"abstract":"<div><h3>Objectives</h3><div>Melioidosis, which is endemic in Malaysia, is caused by <em>Burkholderia pseudomallei</em> and has an elevated risk of mortality. This study reports a case of fatal melioidosis involving an adolescent, where the <em>B. pseudomallei</em> variant causing the infection was traced to the environment based on whole genome sequencing (WGS) analysis.</div></div><div><h3>Methods</h3><div><em>Burkholderia pseudomallei</em> was isolated from an adolescent patient with no known risk factors, who developed fatal community-acquired pneumonia and sepsis. Further investigation and environment sampling were performed to identify the route of infection. Genomic characterization of both clinical and environmental <em>B. pseudomallei</em> isolates was performed to establish genetic relatedness, assess phylogenomic relationships, and identify virulence and antimicrobial resistance genes.</div></div><div><h3>Results</h3><div>Genomic pairwise SNP distance analysis suggests a shared common ancestor between the patient and environmental isolates. Nevertheless, the identification of the novel sequence type ST1901 in both supports the hypothesis of environmental acquisition from a local source. Further analysis identified strain-specific genes that confirmed the strain's regional specificity and virulence. The patient succumbed to the infection despite the isolate being susceptible to the antimicrobials used for melioidosis, and the lack of correlation with antimicrobial resistance gene mutations suggests that treatment failure was not due to resistance. The fatal outcome may have resulted from a delay in initiating effective treatment, leading to the disease progression.</div></div><div><h3>Conclusion</h3><div>This study highlights the effectiveness of WGS in tracing <em>B. pseudomallei</em> infections, which could guide improved diagnostics to enhance melioidosis patient management and treatment outcomes.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"133 ","pages":"Article 105788"},"PeriodicalIF":2.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights into Metapseudomonas otitidis PA-NS83: The first clinical isolate from Thailand and its comparative genomic analysis","authors":"Thunchanok Yaikhan ,&nbsp;Kamonnut Singkhamanan ,&nbsp;Sirikan Suwannasin ,&nbsp;Thitaporn Dechathai ,&nbsp;Mingkwan Yingkajorn ,&nbsp;Sarunyou Chusri ,&nbsp;Komwit Surachat","doi":"10.1016/j.meegid.2025.105786","DOIUrl":"10.1016/j.meegid.2025.105786","url":null,"abstract":"<div><div><em>Metapseudomonas otitidis</em> was first isolated from human middle ear fluid and has since been detected in both environmental and clinical samples, emerging as an opportunistic pathogen linked to chronic otitis media and other infections. This study reports the first clinical isolate of <em>M. otitidis</em> from Thailand, PA-NS83, and presents a comprehensive genomic characterization. Whole-genome sequencing and comparative analysis with 37 publicly available <em>M. otitidis</em> genomes revealed a diverse antimicrobial resistance (AMR) profile, with PA-NS83 carrying AMR genes commonly found in environmental isolates. Virulence gene analysis identified key determinants associated with biofilm formation, motility, secretion systems, and iron acquisition, highlighting its potential pathogenicity.</div><div>Pan-genome analysis demonstrated substantial genomic diversity, with PA-NS83 clustering closely with <em>M. otitidis</em> CSMC7, an environmental isolate from polystyrene waste. However, PA-NS83 harbored 419 unique genes, including virulence-associated genes and a CRISPR-Cas system, suggesting adaptation to clinical settings. These findings underscore the genetic plasticity of <em>M. otitidis</em> and its potential role in human infections. Continued genomic surveillance and functional studies are essential to further assess its clinical significance and antimicrobial resistance mechanisms.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"133 ","pages":"Article 105786"},"PeriodicalIF":2.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular assessment of carbapenem-resistant and ESBL Klebsiella pneumoniae clinical isolates to decipher the correlation of antimicrobial resistance with virulence traits","authors":"Rabia Ilyas ,&nbsp;Sidrah Asghar ,&nbsp;Moatter Zehra ,&nbsp;Yamina Usmani ,&nbsp;Rao Muhammad Abid Khan ,&nbsp;Zulfiqar Ali Mirani ,&nbsp;Syed Abid Ali ,&nbsp;Mohammad Y. Alshahrani ,&nbsp;Ajmal Khan ,&nbsp;Ahmed Al-Harrasi ,&nbsp;Ayaz Ahmed","doi":"10.1016/j.meegid.2025.105785","DOIUrl":"10.1016/j.meegid.2025.105785","url":null,"abstract":"<div><div><em>Klebsiella pneumoniae</em> is a nosocomial pathogen that poses a serious concern due to the high prevalence of extended-spectrum beta-lactamases (<em>ESBL)</em> and carbapenem-resistant <em>K. pneumoniae</em> (<em>CRKP</em>) strains. However, the data on the prevalence of contributing virulence determinants are limited in the Pakistani population. The study aims to characterize clinical isolates of <em>K. pneumoniae</em> to understand clonal relationships and determine the relationship of antibiotic resistance with different virulence factors (i.e., biofilm, capsular polysaccharide, hemolysis, efflux pump, and outer membrane porins). The clinical strains were collected from the diagnostic facility of two public sector hospitals in Karachi. The resistance and virulence profile of the isolates were evaluated via antibiotic susceptibility test, double disk synergy test (DDST), ChromAgar, string test, blood hemolysis, and biofilm assay. Genotypically, the isolates were identified by <em>16S rRNA</em> and <em>rpoB</em> gene, and further characterized for the presence of ESBL, <em>CRKP</em>, biofilm, efflux pump, and outer membrane porins genes. The clonal lineage among isolates was established by Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC PCR). The antibiotic susceptibility test was analyzed through the Multiple Antibiotic Resistance (MAR) index, which revealed 90.2 % (<em>n</em> = 102) strains were MDR. Whereas, the genetic diversity was revealed through ERIC PCR and clade wise data revealed genetic variations due to ESBL (85 %), carbapenem resistance (73 %), biofilm (97 %), efflux pump (40–53 %), outer membrane porins (38–49 %), and hypermucoidity (6 %). The Pearson correlation analysis revealed a strong relationship of MDR strains with biofilm (<em>r</em> = 0.99), efflux pump (<em>r</em> = 0.92), and outer membrane porins (<em>r</em> = 0.88). The study highlighted the prevalence of MDR <em>K. pneumonia</em> with the plethora of virulence factors in the local clinical setting, necessitating stringent screening to develop national policies to tackle further antimicrobial resistance development and outbreaks.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"133 ","pages":"Article 105785"},"PeriodicalIF":2.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic polymorphisms associated with periodontitis in Japanese populations: A comprehensive review of pathways, interactions, and clinical implications","authors":"Elisabetta Ferrara ,&nbsp;Alessandro D'Albenzio ,&nbsp;Biagio Rapone ,&nbsp;Francesco Mastrocola ,&nbsp;Giovanna Murmura","doi":"10.1016/j.meegid.2025.105781","DOIUrl":"10.1016/j.meegid.2025.105781","url":null,"abstract":"<div><div>Periodontitis is a chronic inflammatory disease affecting the supporting structures of teeth, with increasing evidence supporting a significant genetic component in disease susceptibility. This comprehensive review evaluated the associations between genetic polymorphisms and periodontitis in Japanese populations. This narrative review synthesizes available evidence without employing meta-analytical methods. Analysis of relevant studies revealed population-specific genetic architecture, with patterns that suggest possible differences from those observed in Western populations. Significant associations were identified for Japanese populations in immune-related genes (*IL1RN* VNTR: OR 3.40 in G-EOP; *IL1B* -511: OR 1.72 in chronic periodontitis), immunoreceptors (*FCGR3A*-158 V: OR 2.03 in severe chronic periodontitis), tissue remodeling genes (*MMP1* -1607 1G/2G: OR 1.95 in chronic periodontitis), and vitamin D pathway genes (*VDR* +1056 T/C: OR 2.45 in chronic periodontitis). Novel genetic associations with exceptionally strong effect sizes were identified with *ADGRG6* (formerly GPR126) (rs536714306: OR 9.09), *MAEA* (rs6815464: OR 3.73), and *CSF1* genes, expanding our understanding beyond traditional inflammatory pathways. Gene-gene interactions, particularly between *VDR* and *FCGR3B* polymorphisms (composite genotype: OR 5.93), demonstrated substantially stronger associations with periodontitis than individual polymorphisms alone. Protective genetic variants, including *FCGR3B*-NA1 allotype in elderly individuals and *IL1B* rs16944 GA genotype, highlight the concept of genetic resilience. Genetic associations differ markedly between aggressive and chronic forms of periodontitis, with stronger associations typically observed in aggressive/early-onset disease. These findings may contribute to improved risk assessment strategies and personalized approaches to periodontitis prevention and treatment in Japanese individuals, emphasizing the importance of population-specific genetic profiling in periodontal medicine.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"133 ","pages":"Article 105781"},"PeriodicalIF":2.6,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurate species discrimination of Fasciola hepatica from Türkiye based on the fatty acid binding protein type I (FABP type I) gene and phylogenetic analysis using mitochondrial DNA","authors":"Figen Celik ,&nbsp;Michiyo Tashiro ,&nbsp;Sami Simsek ,&nbsp;İbrahim Balkaya ,&nbsp;Yukita Sato ,&nbsp;Madoka Ichikawa-Seki","doi":"10.1016/j.meegid.2025.105783","DOIUrl":"10.1016/j.meegid.2025.105783","url":null,"abstract":"<div><div>Accurate species discrimination based on a robust nuclear protein-coding gene marker is essential for <em>Fasciola</em> spp. because of the presence of <em>F. hepatica</em>, <em>F. gigantica</em>, and hybrid <em>Fasciola</em> flukes that originate from the interspecific hybridization between the two species in Asia. Molecular identification of <em>Fasciola</em> species uses multiplex polymerase chain reaction (PCR) targeting phosphoenolpyruvate carboxykinase (<em>pepck</em>) and PCR-restriction fragment length polymorphism (RFLP) targeting DNA polymerase delta (<em>pold</em>). However, these methods have demonstrated limitations, including misidentification errors. In this study, we aimed to investigate the reliability of multiplex PCR using the fatty acid binding protein type I (<em>FABP type I</em>) gene as a species identification marker. In this study, 75 liver flukes were determined as <em>F. hepatica</em> using <em>FABP type I</em>. <em>FABP type I</em> was more accurate and useful for species identification of <em>F. hepatica</em> than previously reported markers, <em>pepck</em> and <em>pold</em>, as no discrimination errors were observed, whereas misdiagnosis and amplification failure occurred in <em>pepck</em> and <em>pold</em> for five and 13 flukes, respectively. We also aimed to analyze the phylogeny of Turkish <em>Fasciola</em> flukes based on nucleotide sequences of the NADH dehydrogenase subunit 1 (<em>nad1</em>) gene of mitochondrial DNA. Notably, the Turkish <em>F. hepatica</em> population showed greater genetic diversity than the reference populations from the previous studies, suggesting that the Turkish population is much older. This supports the idea that <em>F. hepatica</em> originated in the Fertile Crescent, the area adjacent to Türkiye, as proposed previously.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"133 ","pages":"Article 105783"},"PeriodicalIF":2.6,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Localization of AbaR4-type genomic islands and multidrug resistance plasmids in multiple Acinetobacter baumannii clones and Acinetobacter pittii from infections of dogs and cats.","authors":"Pattrarat Chanchaithong, Chavin Leelapsawas, Parinya Sroithongkham, Jitrapa Yindee, Rapee Thummeepak, Alexandra Collaud, Vincent Perreten","doi":"10.1016/j.meegid.2025.105782","DOIUrl":"https://doi.org/10.1016/j.meegid.2025.105782","url":null,"abstract":"<p><p>The genomes of 39 clinical carbapenemase-producing A. baumannii (CP-Ab) and 2 A. pittii (CP-Ap) isolates from dogs and cats in Thailand (2016-2019) were analyzed for phylogenetic relationship and antimicrobial resistance gene (ARG) localization. Resistome and core genome multilocus sequence typing (cgMLST) analysis identified distinct ARG patterns within CP-Ab sequence type (ST) 2 (n = 4) and ST1575 (n = 1) of clonal complex (CC) 2, ST25 (n = 6), ST1576 (n = 1), and ST1581 (n = 2) of CC25, ST16 (n = 13), ST23 (n = 2), ST149 (n = 9), ST1093 (n = 1), along with CP-Ap (n = 2). Complete genome analysis of one CP-Ap strain and 15 representative CP-Ab strains from each clone identified strains with one to three copies of bla_OXA-23 associated with Tn2006 and/or AbaR-type genomic islands (GIs). AbaR4 was chromosomally located in all other STs except CP-Ab of CC2, which carried AbaR4b. AbaR4 was found on conjugative OXA-23 plasmids in CP-Ab ST1093 and CP-Ap strains. Conjugative OXA-23 plasmids and multidrug resistance (MDR) mega-plasmids in CP-Ab ST149 and CC25 shared ancestry with the cryptic plasmid pCUVET16-467.1 in CP-Ab ST23. MDR mega-plasmids contained a bacteriophage exclusion (BREX) locus and Tn6172-derived elements. A Tn6172ISCR2::(ΔISCR2-ΔTn10-MARR) structure containing GR38 repM, a class 1 integron, and multiple ARGs were found in GR38 MDR mega-plasmids of ST149 and formed an AbGRI1 structure in CC25 plasmids. These findings highlight the role of AbaR4 in carbapenem resistance and the evolution of Acinetobacter plasmids, facilitating the bla_OXA-23 dissemination and the development of extensive drug resistance in multiple clones of clinical CP-Ab and CP-Ap strains originating from companion animals.</p>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":" ","pages":"105782"},"PeriodicalIF":2.6,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144259417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “A highly virulent BA-producing Burkholderia gladioli strain linked to a fatal foodborne outbreak in China, 2020” [Infection, Genetics and Evolution, volume 131, article 105760]","authors":"Jinrui Hu ,&nbsp;Chengyu Xue ,&nbsp;Jinhui Zhang ,&nbsp;Jinyue Liu ,&nbsp;Xiaohua Zheng ,&nbsp;Jing Bai ,&nbsp;Zhigang Cui ,&nbsp;Weiqi Zhong ,&nbsp;Haijian Zhou","doi":"10.1016/j.meegid.2025.105772","DOIUrl":"10.1016/j.meegid.2025.105772","url":null,"abstract":"","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"132 ","pages":"Article 105772"},"PeriodicalIF":2.6,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome sequencing analysis of Burkholderia pseudomallei comparing drug-resistant and pan-susceptible isolates reveals novel biomarkers for drug resistance","authors":"Yothin Hinwan ,&nbsp;Nut Nithimongkolchai ,&nbsp;Kanwara Trisakul ,&nbsp;Benjawan Kaewseekhao ,&nbsp;Lumyai Wonglakorn ,&nbsp;Ploenchan Chetchotisakd ,&nbsp;Sorujsiri Chareonsudjai ,&nbsp;Auttawit Sirichoat ,&nbsp;Arnone Nithichanon ,&nbsp;Jody Phelan ,&nbsp;Taane G. Clark ,&nbsp;Kiatichai Faksri","doi":"10.1016/j.meegid.2025.105779","DOIUrl":"10.1016/j.meegid.2025.105779","url":null,"abstract":"<div><div>Melioidosis, caused by the gram-negative bacterium <em>Burkholderia pseudomallei</em> (Bp), poses a significant health threat due to its potential for drug resistance, which can severely limit available treatment options. To investigate this, we conducted a comparative genomic analysis of 38 drug-resistant (DR) and 300 drug-susceptible (DS) Bp isolates to identify genetic markers associated with antimicrobial resistance. Our study identified seven significant single-nucleotide polymorphisms (SNPs) linked to drug resistance: two with ceftazidime (CAZ), and five with meropenem (MEM). Pathway analysis revealed that AMC resistance was associated with alterations in fatty-acid metabolism, whereas CAZ resistance was associated with changes in membrane protein pathways. These findings highlighted how Bp develops resistance to key antibiotics through various mechanisms. In addition, we discovered 21 novel genetic variants in known drug-resistance genes, including 15 SNPs and six short insertions or deletions (indels). These previously unreported variants could contribute to resistance, highlighting the genetic diversity and adaptability to antimicrobial pressures of Bp. These findings deepen our understanding of Bp drug resistance and offer valuable insights into genetic markers with the potential to enhance diagnostic precision. By enriching the resistance database, this work provides prospective tools for early resistance prediction, facilitating prompt and effective treatment strategies. Furthermore, it emphasizes the critical role of genetic investigations in addressing the challenge of antibiotic resistance in melioidosis.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"133 ","pages":"Article 105779"},"PeriodicalIF":2.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}