Infection Genetics and Evolution最新文献

{"title":"A simple improved method for extracting DNA from ethanol-preserved hard ticks and its applications.","authors":"Nandhini Perumalsamy, Muthukumaravel Subramanian, Rohit Sharma, Ayyanar Elango, Shriram Ananganallur Nagarajan","doi":"10.1016/j.meegid.2024.105709","DOIUrl":"10.1016/j.meegid.2024.105709","url":null,"abstract":"<p><p>Hard tick exoskeletons, composed primarily of chitin, pose a significant challenge for researchers attempting to extract genetic material. This study presents a simple modified, alternative method for extracting DNA from ethanol-preserved hard ticks. The extracted DNA was further used for PCR amplification of phylogenetic markers for population genetics studies. The study also improvises the DNA extraction methods from commercial kits. We have used four DNA extraction methods: Modified Simple Alkaline Lysis, and other commercial kit-based methods (Kit X, Kit Y & Kit Z). The modified method for DNA extraction yielded comparable results in terms of concentration, and purity from all the life stages (adult, nymph, and larvae). The extracted DNA from each method was quantified and subjected to PCR amplification of molecular markers, ITS-1 and ITS-2. The nucleotide sequences from both markers were characterized for the first time and used for phylogenetic analysis of Amblyomma integrum, which is a potential vector for Kyasanur Forest Disease Virus (KFDV), causing monkey fever disease in India. These results demonstrate a cost-effective approach for isolating genomic DNA suitable for PCR amplification and subsequent nucleotide sequencing. Importantly, this simple method offers an option for population genetics study in resource-limited settings, facilitating field research with minimal equipment requirements. Additionally, the study showed tick homogenization can significantly improve DNA yield from commercial kits.</p>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":" ","pages":"105709"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BCG's role in strengthening immune responses: Implications for tuberculosis and comorbid diseases.","authors":"Nilofer Naqvi, Yashika Ahuja, Sheeba Zarin, Anwar Alam, Waseem Ali, Mohd Shariq, Seyed E Hasnain, Nasreen Z Ehtesham","doi":"10.1016/j.meegid.2024.105703","DOIUrl":"10.1016/j.meegid.2024.105703","url":null,"abstract":"<p><p>The BCG vaccine represents a significant milestone in the prevention of tuberculosis (TB), particularly in children. Researchers have been developing recombinant BCG (rBCG) variants that can trigger lasting memory responses, thereby enhancing protection against TB in adults. The breakdown of immune surveillance is a key link between TB and other communicable and non-communicable diseases. Notably, TB is more prevalent among people with comorbidities such as HIV, diabetes, cancer, influenza, COVID-19, and autoimmune disorders. rBCG formulations have the potential to address both TB and HIV co-pandemics. TB increases the risk of lung cancer and immunosuppression caused by cancer can reactivate latent TB infections. Moreover, BCG's efficacy extends to bladder cancer treatment and blood glucose regulation in patients with diabetes and TB. Additionally, BCG provides cross-protection against unrelated pathogens, emphasizing the importance of BCG-induced trained immunity in COVID-19 and other respiratory diseases. Furthermore, BCG reduced the severity of pulmonary TB-induced influenza virus infections. Recent studies have proposed innovations in BCG delivery, revaccination, and attenuation techniques. Disease-centered research has highlighted the immunomodulatory effects of BCG on TB, HIV, cancer, diabetes, COVID-19, and autoimmune diseases. The complex relationship between TB and comorbidities requires a nuanced re-evaluation to understand the shared attributes regulated by BCG. This review assessed the interconnected relationships influenced by BCG administration in TB and related disorders, recommending the expanded use of rBCG in healthcare. Collaboration among vaccine research stakeholders is vital to enhance BCG's efficacy against global health challenges.</p>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":" ","pages":"105703"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dengue virus: Etiology, epidemiology, pathobiology, and developments in diagnosis and control - A comprehensive review.","authors":"Masoud Pourzangiabadi, Hamideh Najafi, Arezoo Fallah, Aida Goudarzi, Iman Pouladi","doi":"10.1016/j.meegid.2024.105710","DOIUrl":"10.1016/j.meegid.2024.105710","url":null,"abstract":"<p><p>Dengue flavivirus (DENV) is the virus that causes dengue, one of the most dangerous and common viral diseases in humans that are carried by mosquitoes and can lead to fatalities. Every year, there are over 400 million cases of dengue fever worldwide, and 22,000 fatalities. It has been documented in tropical and subtropical climates in over 100 nations. Unfortunately, there is no specific treatment approach, but prevention, adequate awareness, diagnosis in the early stages of viral infection and proper medical care can reduce the mortality rate. The first licensed vaccine for dengue virus (CYD Denvaxia) was quadrivalent, but it is not approved in all countries. The primary barriers to vaccine development include inadequate animal models, inadequate etiology mechanistic studies, and adverse drug events. This study provides current knowledge and a comprehensive view of the biology, production and reproduction, transmission, pathogenesis and diagnosis, epidemiology and control measures of dengue virus.</p>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":" ","pages":"105710"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Europe-wide distribution and bat-host specific lineages in the malarial parasite Polychromophilus murinus revealed through genetic screening of bat flies.","authors":"Luisa Timm, Sascha P Rosskopf, Oskar Werb, Jaap van Schaik, Juliane Schaer","doi":"10.1016/j.meegid.2024.105707","DOIUrl":"10.1016/j.meegid.2024.105707","url":null,"abstract":"<p><p>Malaria parasites of the genus Polychromophilus commonly infect vespertilionid and miniopterid bats, and are transmitted by bat flies (Nycteribiidae). While Polychromophilus murinus has been recorded sporadically in Europe, its host range, distribution and phylogeographic structure have not been explored. Here we investigate the prevalence and genetic diversity of P. murinus infections in 1131 bat flies collected from seven European bat species, focusing on Basilia nana, collected from its primary host, the Bechstein's bat throughout its distribution. Additionally, we explore the temporal dynamics of P. murinus in two regions in Germany where bat flies were repeatedly collected over at least five years. Bat flies were screened for Polychromophilus infection via PCR of a fragment of cytochrome b, and fragments of three additional genes were sequenced for positive samples. Overall, P. murinus infections were detected in 287 of 1131 screened bat flies of four different species, collected from seven bat species, across 13 countries. The 269 recovered cytb sequences represented 21 distinct haplotypes, clustered based on the bat species from which the infected flies were collected. Repeated sampling over multiple years revealed a consistent presence of P. murinus in both investigated populations, without substantial variation in prevalence between years. The results suggest an endemic long-term presence of Polychromophilus within European bat populations, and the presence of host-specific associations between P. murinus lineages and its various bat hosts. We posit that exposure to P. murinus appears to be a near certainty in several European bat species, and its potential costs should be further investigated.</p>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"127 ","pages":"105707"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Narrowing the region of candidate genes that control the development of protoscoleces of Echinococcus multilocularis in the mouse liver.","authors":"Keisuke Sato, Naritaka Hikosaka, Hirokazu Kouguchi, Takao Irie, Masami Morimatsu, Takashi Agui","doi":"10.1016/j.meegid.2024.105704","DOIUrl":"10.1016/j.meegid.2024.105704","url":null,"abstract":"<p><p>Alveolar echinococcosis is a zoonosis caused by the larval stage of Echinococcus multilocularis. In previous studies, QTL analysis using C57BL/6 N (B6) and DBA/2 (D2) which differ in susceptibility suggested the presence of genes on chromosome 1 that control protoscolex development. In this study, we constructed several congenic mice with different chromosome 1 regions substituted to confirm the presence of responsible genes and to narrow down the regions where the responsible genes exist. Five lines of third-generation congenic strain were constructed and infection experiments were conducted. The results showed that the development of protoscolex was seen in the two lines, resulting to narrow-down the responsible region between 69.4 cM and 70.67 cM. There were 18 genes having different SNPs and 10 genes having amino acid substitutions between B6 and D2 within this region. Infection experiments with third-generation of congenic mice succeeded in narrowing down the chromosomal region determining protoscolex development, resulting to reduce the number of candidate genes. The identification of the gene responsible for protoscolex development will contribute to the control of E. multilocularis infection.</p>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":" ","pages":"105704"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142808685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid confirmation of autochthonous origin in suspected cases of melioidosis from French overseas departments in the Caribbean and the Indian Ocean by PCR-high resolution melting (HRM) analysis.","authors":"Mégane Gasqué, Vanina Guernier-Cambert, Guillaume Girault, Jules Terret, Fabienne Neulat-Ripoll, Emma Rochelle-Newall, Karine Laroucau","doi":"10.1016/j.meegid.2024.105711","DOIUrl":"10.1016/j.meegid.2024.105711","url":null,"abstract":"<p><p>Burkholderia pseudomallei, a soil-borne bacterium that causes melioidosis, endemic in South and Southeast Asia and northern Australia, is now emerging in new regions. Since the 1990s, cases have been reported in French overseas departments, including Martinique and Guadeloupe in the Caribbean, and Reunion Island and Mayotte in the Indian Ocean, suggesting a local presence of the bacterium. Our phylogenetic analysis of 111 B. pseudomallei genomes isolated worldwide, including three strains from Martinique, revealed distinct geography-specific clades for Africa, the Americas, Asia and Australasia. Single nucleotide polymorphisms (SNP) that define clade branches in the phylogeny were identified; we selected those specific to three regions relevant to the French overseas departments: the Indian Ocean, the Americas and a unique subset specific to Martinique. Three SNP markers (one per region) were used to develop a PCR-high resolution melting tool to discriminate between local and imported strains in each region. Blind tests on B. pseudomallei strains from French patients, from overseas departments and mainland France, were used for validation. Our method accurately predicted the geographic origin of the patient as recorded from the patient travel history and/or from the multilocus sequence typing data. This rapid typing method, which allows timely identification of local cases and targeted public health interventions, is particularly valuable in the French overseas departments where melioidosis is emerging and regulatory constraints limit the handling of B. pseudomallei. Although initially tailored to specific regions, this tool can be adapted for use in other areas to support local epidemiological surveillance of melioidosis.</p>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":" ","pages":"105711"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying the unknown: Application of molecular epidemiology tools to identify clustering and HIV transmission routes in Poland.","authors":"Marcin Horecki, Karol Serwin, Iwona Cielniak, Ewa Siwak, Monika Bociąga Jasik, Anna Kalinowska-Nowak, Błażej Rozpłochowski, Bogusz Aksak-Wąs, Magdalena Witak-Jędra, Aleksandra Szymczak, Bartosz Szetela, Elżbieta Mularska, Adam Witor, Paweł Jakubowski, Maria Hlebowicz, Anita Olczak, Władysław Łojewski, Elżbieta Jabłonowska, Kaja Mielczak, Piotr Ząbek, Miłosz Parczewski","doi":"10.1016/j.meegid.2024.105699","DOIUrl":"https://doi.org/10.1016/j.meegid.2024.105699","url":null,"abstract":"<p><strong>Background: </strong>Understanding the dynamics of HIV-1 transmission is essential for developing effective screening and intervention strategies. Viral genetic sequences provide valuable information that can be used to infer the history and patterns of viral transmission.</p><p><strong>Purpose: </strong>Our study explores the structure and dynamics of HIV transmissions in Poland from 1999 to 2022 to elucidate key patterns related with national epidemics.</p><p><strong>Methods: </strong>To understand the temporal dynamics of transmission routes we examined HIV pol sequence data from 5705 Polish PWH. The HIV-TRAnsmission Cluster Engine (HIV-TRACE) was utilized to identify potential links between different risk groups and putative links to individuals with unreported transmission risk.</p><p><strong>Results: </strong>Our analyses generated 503 clusters, containing 3942 individuals, and identified 13,917 putative links. Approximately 69.1 % of the sequences formed clusters. In the dataset 32.2 % of individuals were reported MSM transmission route, 7.9 % by heterosexual, and 5.6 % by PWID transmissions. The transmission route was unknown for 54.2 % of patients. Putative transmissions from MSM to all other groups revealed that 45.1 % of links lead to people with unregistered transmission mode. For heterosexual patients, 40.2 % of connections were directed to patients lacking information on infection routes and 30.5 % to MSM individuals. Our analysis unveiled that 45.1 % of cases with unreported transmission routes may be identified as MSM, while 3.5 % might be potential non-disclosed MSM.</p><p><strong>Conclusions: </strong>Genetic linkages can provide valuable insights into the transmission dynamics among individuals, even in cases where transmission risk information is missing or unreported. The observed association between MSM and unreported cases highlights the potential of molecular epidemiology to complete missing patient data.</p>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":" ","pages":"105699"},"PeriodicalIF":2.6,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analysis of blaNDM-1-carrying-Pseudomonas aeruginosa ST2407 in the chromosome from Brazil.","authors":"Ivson Cassiano de Oliveira Santos, Melise Chaves Silveira, Daiana Cristina Silva Rodrigues, Bruna Ribeiro Sued-Karam, Bruno Rocha Pribul, Giovanna de Oliveira Santos, Jônathas Dias Nunes, Marcos Dornelas-Ribeiro, Gabriela Bergiante Kraychete, Renata Cristina Picão, Elizabeth Andrade Marques, Robson Souza Leão, Cláudio Marcos Rocha-de-Souza, Ana Paula D'Alincourt Carvalho-Assef","doi":"10.1016/j.meegid.2024.105702","DOIUrl":"10.1016/j.meegid.2024.105702","url":null,"abstract":"<p><p>Pseudomonas aeruginosa, an opportunistic pathogen often found in Healthcare-associated infections (HAI), has shown increased resistance to carbapenems (imipenem, meropenem, doripenem), the primary treatment options. We've seen a rise in carbapenemase-producing P. aeruginosa in Brazil, including NDM-producers. This study characterises an isolate carrying bla_NDM-1 from a patient's skin fragment in a Brazilian hospital. The whole genomic sequence (WGS) of P. aeruginosa CCBH26428 was extracted and sequenced using Illumina and minION platforms. The assembly used MinION results mapped with Illumina reads, and annotation was performed by the RAST server. Resistance genes and clonality were identified using the CABGen platform. Additional information was carried out by manual annotation using Geneious software and BLAST tool. The genomic analysis revealed a genome of 6.995.008 bp and G+C 65.9 %. P. aeruginosa CCBH26428 belongs to ST2407. The bla_NDM gene, associated with ISAba125, was found in a 63.862 pb genomic region flanked by IS26 insertion sequences. This region also contained the repA of the plasmid incompatibility group IncC2 and other resistance genes, suggesting it is a possible \"translocation unit\". Additionally, 17 resistance genes, mutations in OprD and GyrA, and several virulence genes were detected, potentially exacerbating the infection. This study is report a WGS analysis of P. aeruginosa carrying bla_NDM-1 in Brazil, highlighting the role of IS26 in the acquisition and spread of resistance genes between plasmids and chromosomes.</p>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":" ","pages":"105702"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142808683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic and phylodynamic analysis of respiratory syncytial virus strains circulating in children less than five years of age in Karachi-Pakistan","authors":"Fatima Aziz ,&nbsp;Nida Farooqui ,&nbsp;Tanveer Abbas ,&nbsp;Mahnoor Javaid ,&nbsp;Wardah Rafaqat ,&nbsp;Alnara Zhamalbekova ,&nbsp;Syed Asad Ali ,&nbsp;Syed Ali ,&nbsp;Syed Hani Abid","doi":"10.1016/j.meegid.2024.105694","DOIUrl":"10.1016/j.meegid.2024.105694","url":null,"abstract":"<div><h3>Background</h3><div>Respiratory syncytial virus (RSV) is one of the leading causes of infant morbidity and mortality worldwide, especially in Pakistan. To date, few studies have explored RSV epidemiology in different areas of Pakistan. However, none have performed comprehensive phylogenetic and phylodynamic analyses of RSV strains. This study presents a comprehensive genetic and phylodynamic analysis of RSV strains in children less than five years old in Karachi, Pakistan.</div></div><div><h3>Methods</h3><div>This study used retrospectively collected nasopharyngeal (swab) samples from 155 children with qPCR-confirmed RSV infection. The samples were used to perform RSV genotyping using PCR employing RSV glycoprotein gene-specific primers. The RSVA and RSVB genotyping was performed using BLAST and Maximum-likelihood (ML) phylogenetic methods. Similarly, the relationship with other RSV strains was analyzed using ML phylogenetic cluster analysis. The RSVA and RSVB mean genetic diversity and coefficient of differentiation were calculated using MEGA7 software. Furthermore, the time to the most common recent ancestor (tMRCA) and effective population size of RSV genotypes A and B were estimated using a Bayesian MCMC analysis. Finally, site selection pressure and glycosylation analyses were performed using FUBAR and NetNGlyc/NetOGlyc tools.</div></div><div><h3>Results</h3><div>Out of 155, 98 and 57 sequences were RSVA and RSVB, respectively. The tMRCA was estimated to be around 2002 and 2005 for RSVA and RSVB, respectively. RSVA sequences formed two NA1 genotype clusters, comprising 95 and three sequences, respectively. RSVB formed three clusters, where 24 and two sequences clustered with BA9 and BA12 genotypes, respectively, while 31 sequences formed a unique cluster. The RSVA and RSVB glycoprotein gene sequences exhibited N- and O- glycosylation and selection pressure at several sites. RSV B exhibited slightly higher (0.042) nucleotide diversity per site (π) as compared to RSVA (0.019).</div></div><div><h3>Conclusions</h3><div>Our results suggest that RSVA and RSVB strains in Pakistan exhibit distinct genotypic clusters and differ in their estimated tMRCA. Additionally, both genotypes showed glycosylation and selection pressure at specific sites, with RSVB exhibiting higher nucleotide divergence per site (π), indicating its potential to undergo further evolutionary changes and adaptation. Overall, this study provides unique insights into RSV molecular epidemiology. The study may also help improve our understanding of RSV evolutionary changes and the emergence of new genotypes in different regions worldwide and within Pakistan.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"126 ","pages":"Article 105694"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Epidemiological and phylogenetic characteristics of emerging Anaplasma capra: A systematic review with modeling analysis\" [Infection, Genetics and Evolution, volume 115, article 105510].","authors":"Zhe-Tao Lin, Run-Ze Ye, Jin-Yue Liu, Xiao-Yang Wang, Wen-Jie Zhu, Yu-Yu Li, Xiao-Ming Cui, Wu-Chun Cao","doi":"10.1016/j.meegid.2024.105696","DOIUrl":"10.1016/j.meegid.2024.105696","url":null,"abstract":"","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":" ","pages":"105696"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}