Infection Genetics and Evolution最新文献_第3页

Metabarcoding characterization of gastrointestinal strongyle nematodes in captive Asian elephants (Elephas maximus) and white rhinoceroses (Ceratotherium simum) in a private zoo, Thailand 泰国一家私人动物园圈养亚洲象（大象）和白犀牛（白犀牛）胃肠道圆形线虫的元条形码特征

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-08-30 DOI: 10.1016/j.meegid.2025.105817

Mohamed H. Hamad , Witchuta Junsiri , Tanagrit Sumpanpae , Duriyang Narapakdeesakul , Piyanan Taweethavonsawat

{"title":"Metabarcoding characterization of gastrointestinal strongyle nematodes in captive Asian elephants (Elephas maximus) and white rhinoceroses (Ceratotherium simum) in a private zoo, Thailand","authors":"Mohamed H. Hamad , Witchuta Junsiri , Tanagrit Sumpanpae , Duriyang Narapakdeesakul , Piyanan Taweethavonsawat","doi":"10.1016/j.meegid.2025.105817","DOIUrl":"10.1016/j.meegid.2025.105817","url":null,"abstract":"<div><div>Gastrointestinal strongyle nematodes pose significant health risks to captive megaherbivores, including Asian elephants (<em>Elephas maximus</em>) and white rhinoceroses (<em>Ceratotherium simum</em>). Traditional diagnostic methods often fail to accurately identify species due to morphological similarities, limiting understanding of parasite diversity and host-specificity. This study is among the first in Southeast Asia to apply high-throughput internal transcribed spacer-2 (ITS-2) rDNA metabarcoding to characterize strongyle nematode communities in these endangered hosts. Fecal samples from six rhinoceroses and four elephants housed in a private zoo in Thailand were processed using flotation, larval culture, and DNA extraction protocols. Amplicon sequencing was conducted on the Illumina MiSeq platform, and taxonomic assignments were performed using the DADA2 pipeline and NCBI/GenBank databases. Our results revealed the presence of strongyle infections. <em>Murshidia</em> spp. were detected in both host species, while <em>Kiluluma ceratotherii</em> was found exclusively in rhinoceroses. Phylogenetic analysis based on ITS-2 rDNA sequences demonstrated clear host-associated clades and suggested potential cryptic species within <em>Kiluluma</em> and <em>Murshidia</em> lineages. These findings provide new genetic evidence of host specificity and evolutionary divergence among strongylid nematodes in captive wildlife. The study underscores the utility of DNA metabarcoding for non-invasive parasite surveillance and highlights the urgent need to expand molecular databases for better taxonomic resolution in wildlife parasitology.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"134 ","pages":"Article 105817"},"PeriodicalIF":2.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144921659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole genome sequencing and molecular characterization of porcine astrovirus from Haryana, India 印度哈里亚纳邦猪星状病毒的全基因组测序和分子特征

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-08-27 DOI: 10.1016/j.meegid.2025.105816

Ritu Panghal , Parveen Kumar , Sanjeevna K. Minhas , Raman Mehtani , Sweety Kalonia , Deepika Sheoran , Pawan Kumar , Akhil K. Gupta , Rajesh Chhabra , Naresh Jindal

{"title":"Whole genome sequencing and molecular characterization of porcine astrovirus from Haryana, India","authors":"Ritu Panghal , Parveen Kumar , Sanjeevna K. Minhas , Raman Mehtani , Sweety Kalonia , Deepika Sheoran , Pawan Kumar , Akhil K. Gupta , Rajesh Chhabra , Naresh Jindal","doi":"10.1016/j.meegid.2025.105816","DOIUrl":"10.1016/j.meegid.2025.105816","url":null,"abstract":"<div><div>Porcine astrovirus (PAstV) is an enteropathogen that belongs to the family <em>Astroviridae</em> and the genus <em>Mamastrovirus,</em> and is divided into five distinct genotypes (PAstV1-PAstV5). PAstV has been reported in pigs from several countries worldwide. In India, there are limited reports of genetic characterization of PAstV. The present study aimed to characterize the whole genome of PAstV from Haryana, a state in Northern India. Initially, 70 porcine fecal samples were screened for the presence of PAstV using RT-PCR that targeted the partial RdRp region. Three PAstV-positive samples were selected and their complete genomes successfully amplified by RT-PCR in two overlapping segments: segment A (∼3.6 kb) and segment B (∼3.1 kb). The phylogenetic analysis of PAstV, based on complete nucleotide sequences, revealed that PV247173 and PV247174 clustered within the PAstV1 group, whereas PV455027 clustered within the PAstV4 group. In contrast, analysis of the ORF2 amino acid sequence revealed that only PV247173 was classified in PAstV1, while PV455027 and PV247174 were classified in PAstV4. This study reports the first whole-genome molecular characterization of PAstV from Northern India.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"134 ","pages":"Article 105816"},"PeriodicalIF":2.6,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic characterization and serotype distribution of dengue virus circulating in Pakistan during 2024 2024年巴基斯坦流行登革热病毒的基因组特征和血清型分布

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-08-26 DOI: 10.1016/j.meegid.2025.105815

Zunera Jamal , Fahad Humayun , Syed Adnan Haider , Rabia Hakim , Verity Hill , Syed Yawar Saeed , Guy Baele , Muhammad Ishaq , Mansur Ayub , Amjad Ullah , Nasir Hussain , Muhammad Salman , Massab Umair

{"title":"Genomic characterization and serotype distribution of dengue virus circulating in Pakistan during 2024","authors":"Zunera Jamal , Fahad Humayun , Syed Adnan Haider , Rabia Hakim , Verity Hill , Syed Yawar Saeed , Guy Baele , Muhammad Ishaq , Mansur Ayub , Amjad Ullah , Nasir Hussain , Muhammad Salman , Massab Umair","doi":"10.1016/j.meegid.2025.105815","DOIUrl":"10.1016/j.meegid.2025.105815","url":null,"abstract":"<div><div>Dengue virus (DENV) remains a significant public health concern in Pakistan, with recurrent outbreaks necessitating continuous genomic surveillance. The 2024 dengue outbreak prompted an investigation into circulating serotypes and genomic diversity. The National Institute of Health (NIH), Pakistan, received 524 NS-1 confirmed dengue samples across multiple districts in 2024. Serotyping via real-time PCR confirmed DENV-2 in 93 % (<em>n</em> = 361) and DENV-1 in 7 % (<em>n</em> = 27) of 388 positive samples, with no co-infections detected. Males accounted for 65 % of cases, with a mean age of 30.78 ± 15.03 years. Metagenomic sequencing using Illumina MiSeq yielded 33 successful DENV genomes (DENV-2: 23; DENV-1: 10), including the first reports from Chilas and Kech. Phylogenetic analysis showed 2024 DENV-1 (genotype III) and DENV-2 (Cosmopolitan genotype, clade IV-A/2II_F.1.1) closely resembling 2022–2023 Pakistani strains (∼99 % homology). Time-scaled analysis estimated 22 of 23 DENV-2 sequences shared a most recent common ancestor (MRCA) in 2018, while a divergent strain (NIHPAK-246) traced back to 2005. DENV-2 strains from Kech exhibited >99.8 % similarity with Rawalpindi strains, suggesting transmission links. DENV-1 sequences shared an MRCA in 2016, indicating sustained circulation. Mutation analysis identified NS4B: G240S in DENV-1 from Chilas, suggesting localized adaptation. These findings highlight the need for sustained genomic surveillance, monitoring transmission dynamics, and targeted public health interventions in Pakistan.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"134 ","pages":"Article 105815"},"PeriodicalIF":2.6,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnosing plague in 17th century camelids from Romania along historical Silk Routes 17世纪诊断鼠疫的骆驼来自罗马尼亚沿着历史丝绸之路

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-08-22 DOI: 10.1016/j.meegid.2025.105814

Hamadou Oumarou Hama , Mahmoud Boualam , Jean Armengaud , Michel Drancourt , Gérard Aboudharam , Adrian Bălășescu , Valentin Radu

引用次数: 0

Comparative and phylogenomic analysis of Chlamydia pneumoniae reveals unique carbohydrate active enzyme family (GT5) among respiratory isolates 肺炎衣原体的比较和系统基因组分析揭示了呼吸分离株中独特的碳水化合物活性酶家族（GT5）

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-08-21 DOI: 10.1016/j.meegid.2025.105813

Susanthika Jayachandiran, Roja Suresh, Ramasamy Dhamodharan

{"title":"Comparative and phylogenomic analysis of Chlamydia pneumoniae reveals unique carbohydrate active enzyme family (GT5) among respiratory isolates","authors":"Susanthika Jayachandiran, Roja Suresh, Ramasamy Dhamodharan","doi":"10.1016/j.meegid.2025.105813","DOIUrl":"10.1016/j.meegid.2025.105813","url":null,"abstract":"<div><div><em>Chlamydia pneumoniae</em> is an obligatory intracellular pathogen found in humans and animals. Understanding the genomic diversity is crucial for unravelling its pathogenic mechanisms and transmission dynamics. In this study, 14 complete genomes of <em>C. pneumoniae</em> strains were compared for functional diversity analysis. The koala isolate LPCoLN appears as a phylogenetically distinct, showing the fewest accessory genes and the highest incorporation of unique or absent genes among the strains analyzed. Functional annotation indicates that certain metabolic pathways between the LPCoLN and the human respiratory strain AR39 were the same, which is most likely due to phage-associated elements present in AR39. The presence of the GT5 CAZyme family is significantly associated with strains of respiratory origin, suggesting a potential role in respiratory adaptation and pathogenic strategies, including tissue colonization, immune evasion, and niche-specific persistence. The strong association between GT5 CAZymes and respiratory-origin strains highlights their potential as diagnostic markers and therapeutic targets.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"134 ","pages":"Article 105813"},"PeriodicalIF":2.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intestinal microbiota diversity in children from three African countries 三个非洲国家儿童肠道菌群多样性

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-08-18 DOI: 10.1016/j.meegid.2025.105808

Catherine Dauga , Valentine Gilbart , Azimdine Habib , Laura Tondeur , Rindra Randremanana , Boris Hedible , Alexandre Manirazika , Muriel Vray , Ronan Jambou , the Malinea Clinical Trial Group

{"title":"Intestinal microbiota diversity in children from three African countries","authors":"Catherine Dauga , Valentine Gilbart , Azimdine Habib , Laura Tondeur , Rindra Randremanana , Boris Hedible , Alexandre Manirazika , Muriel Vray , Ronan Jambou , the Malinea Clinical Trial Group","doi":"10.1016/j.meegid.2025.105808","DOIUrl":"10.1016/j.meegid.2025.105808","url":null,"abstract":"<div><div>In children, the gut microbiota is a dynamic ecosystem derived from breastfeeding, oral microbiota and diet. Diversity can vary from one country to another, and the aim of this study was to compare microbiota of well-nourished children between three African countries using fully standardized protocols from sampling to data analysis.</div><div>Well-nourished (WHZ weight for height z-score ≥ −1.5) children aged 18–24 months were enrolled. Libraries were constructed using Illumina 16S protocol. Sequences were aligned to the Silva.nr and clustered into OTUs (organism taxonomic unit) using greedy clustering (dgc). Consensus determination was done using classify.otu and the OTU table, taxonomy and metadata were assembled using Phyloseq. Alpha diversity was assessed using Chao1, Shannon and Simpson's index using Mothur.</div><div>106 well-nourished children were selected (31 in Madagascar, 40 in Senegal and 35 in Centrafrican Republic CAR). Richness was higher in Senegal than in CAR or Madagascar, but diversity was lowest in Senegal and highest in CAR. As the quality of drinking water increased, richness increased and diversity decreased. Microbiota shared five dominant phyla in different proportions: <em>Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, Verrucomicrobia</em>. The composition of the microbiota in Madagascar and Centrafrica republic (CAR) was more similar, with more <em>Prevotella 9</em> in Madagascar and more <em>Bifidobacterium</em> in CAR. Only two taxa were markers from CAR (<em>Prevotella 9</em> and <em>Ruminococcus 2</em>).</div><div>Apart from a pool of common species, a large proportion of rare species may characterize each geographical context. Therefore, the microbiota of children in Africa cannot be considered comparable between countries. Each biotope harbors specific species on a common background.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"134 ","pages":"Article 105808"},"PeriodicalIF":2.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Next-generation sequencing of Omicron SARS-CoV-2 variants in Hormozgan Province, Iran, and evaluation of the effects of mutations on RBD and ORF9b protein function 伊朗霍尔木兹甘省SARS-CoV-2 Omicron变异的新一代测序及突变对RBD和ORF9b蛋白功能的影响

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-08-12 DOI: 10.1016/j.meegid.2025.105812

Khadijeh Ahmadi , Soudabeh Kavousipour , Behzad Shahbazi , Farhad Farzin , Zahra Gharibi , Maryam Sadat Pishva , Ali Pishahang , Hamed Gouklani

{"title":"Next-generation sequencing of Omicron SARS-CoV-2 variants in Hormozgan Province, Iran, and evaluation of the effects of mutations on RBD and ORF9b protein function","authors":"Khadijeh Ahmadi , Soudabeh Kavousipour , Behzad Shahbazi , Farhad Farzin , Zahra Gharibi , Maryam Sadat Pishva , Ali Pishahang , Hamed Gouklani","doi":"10.1016/j.meegid.2025.105812","DOIUrl":"10.1016/j.meegid.2025.105812","url":null,"abstract":"<div><div>The emergence of the Omicron variant in November 2021 marked a significant turning point in the COVID-19 pandemic due to its unprecedented number of mutations compared to previous variants. To better understand its clinical impact and evolutionary trajectory, we conducted a comprehensive genomic epidemiology study analyzing 528 <em>SARS-CoV-2</em> samples collected in Iran between March 2021 and March 2023. Using nanopore sequencing with ≥96 % genome coverage and advanced bioinformatics tools including the Nextclade platform, we systematically characterized the mutational profiles of circulating variants with particular focus on the receptor-binding domain (RBD) and ORF9b proteins. Our analysis revealed BA.5.2 as the dominant strain (32.4 % prevalence), followed by XBB.1.9.1 (14.2 %), with only 17.6 % of cases occurring in vaccinated individuals. Through molecular docking and dynamics simulations, we demonstrated that key variants including BA.5.2, XBB.1.5, and XBB.1.9 exhibit enhanced binding affinity to host receptors, with RBD showing stronger interactions with ACE2 and ORF9b variants displaying improved binding to TOM70. Notably, our findings suggest similar pathogenic potential between the XBB.1.5/XBB.1.9 and BA.5 lineages, while highlighting the utility of whole genome entropy analysis for predicting viral evolution patterns. These results provide valuable insights for guiding vaccine development, therapeutic design, and public health strategies against evolving <em>SARS-CoV-2</em> variants.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"134 ","pages":"Article 105812"},"PeriodicalIF":2.6,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144852812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the pathogenicity of Clostridium perfringens alpha toxin 产气荚膜梭菌α毒素致病性的研究。

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-08-09 DOI: 10.1016/j.meegid.2025.105811

Chongli Xu , Yuhan She , Hongli Li , Ling Hou , Wenqiao Ding

{"title":"Study on the pathogenicity of Clostridium perfringens alpha toxin","authors":"Chongli Xu , Yuhan She , Hongli Li , Ling Hou , Wenqiao Ding","doi":"10.1016/j.meegid.2025.105811","DOIUrl":"10.1016/j.meegid.2025.105811","url":null,"abstract":"<div><div>Alpha toxin is one of the primary pathogenic factors of <em>Clostridium perfringens</em> and is a metalloenzyme with phospholipase C activity. Currently, the pathogenic mechanism of α toxin is not fully understood. The pathogenicity and significance of α toxin in all toxin-type <em>Clostridium perfringens</em> has not been fully studied. The results of circular dichroism spectroscopy of the α toxin protein at different temperatures in this study showed that the molecular structure of the α toxin protein changed at various temperatures, leading to changes in its biological activity. These findings indicate that the molecular structure of α toxin is essential for maintaining its biological activity. Additionally, recombinant proteins of α toxin, β<sub>1</sub> toxin, β<sub>2</sub> toxin and ε toxin were individually cloned and expressed. Simultaneously, recombinant protein immunization experiments and animal challenge experiments were conducted. The significant role of alpha toxin in the pathogenicity of various types of <em>Clostridium perfringens</em> has been established, providing mechanistic insight into α toxin function and the development of genetic engineering subunit vaccines in the future.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"134 ","pages":"Article 105811"},"PeriodicalIF":2.6,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144823266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in the interaction between acute respiratory distress syndrome and gut microbiota: A narrative review 急性呼吸窘迫综合征与肠道菌群相互作用的最新进展：综述。

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-08-09 DOI: 10.1016/j.meegid.2025.105810

Xiao-Qing Fan , Shi-Ming Fan , Bi-Ying Dong , Chun-Mei Zhang , Jing Zuo , Dong-Wei Zhang , Xia Xiong , Dan Luo , Xian-Ming Fan

{"title":"Recent advances in the interaction between acute respiratory distress syndrome and gut microbiota: A narrative review","authors":"Xiao-Qing Fan , Shi-Ming Fan , Bi-Ying Dong , Chun-Mei Zhang , Jing Zuo , Dong-Wei Zhang , Xia Xiong , Dan Luo , Xian-Ming Fan","doi":"10.1016/j.meegid.2025.105810","DOIUrl":"10.1016/j.meegid.2025.105810","url":null,"abstract":"<div><div>Acute respiratory distress syndrome (ARDS) is a sudden, widespread inflammatory damage to the lungs resulting from multiple etiologies. ARDS is characterized by high sickness rates, mortality, and costly treatments and is a significant global health issue that lacks an effective treatment strategy. The microbiota of the gut is an intricate ecology indispensable for the host's health, immunology, and metabolism. Human immunity and intestinal barrier function depend on gut microbes. Several disorders are linked to gut microbiota dysbiosis. Scientists have been concentrating on the role that gut microbes play in the onset of ARDS. This study examines the relationship between ARDS and intestinal microbiota, specifically addressing two facets: how ARDS affects the composition of the gut microbiota and the integrity of the intestinal barrier, alongside the effects of mechanisms such as bacterial translocation and inflammatory activation resulting from gut microbiota dysregulation on ARDS. Additionally, various therapeutic strategies involving gut microbiota and its metabolites, such as selective digestive decontamination (SDD), fecal microbiota transplantation (FMT), microbiological preparations, and metabolites produced from the microbiota, are explored. It is anticipated that this exploration will make a substantial contribution to the prevention and therapy of ARDS.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"134 ","pages":"Article 105810"},"PeriodicalIF":2.6,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144823265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening for Borna disease virus 1 (BoDV-1) in Austria: Absence of human cases in a retrospective case-finding study 奥地利博尔纳病病毒1型（BoDV-1）筛查：回顾性病例发现研究中未发现人类病例。

IF 2.6 4区医学

Infection Genetics and Evolution Pub Date : 2025-07-29 DOI: 10.1016/j.meegid.2025.105806

Markus Bauswein , Lisa Arnold , David N. Springer , Monika Redlberger-Fritz

{"title":"Screening for Borna disease virus 1 (BoDV-1) in Austria: Absence of human cases in a retrospective case-finding study","authors":"Markus Bauswein , Lisa Arnold , David N. Springer , Monika Redlberger-Fritz","doi":"10.1016/j.meegid.2025.105806","DOIUrl":"10.1016/j.meegid.2025.105806","url":null,"abstract":"<div><h3>Background</h3><div>Borna disease virus 1 (BoDV-1) is a zoonotic virus with a recently confirmed potential to cause rare but severe cases of encephalitis in humans. While the bicolored white-toothed shrew (<em>Crocidura leucodon</em>), which represents the reservoir, is widely distributed over eastern, central, and southern Europe as well as south-west Asia, human infections have so far only been reported from Germany. As infections in sentinels such as horses indicate the endemic circulation of the virus also in circumscribed regions of neighboring countries (Austria, Liechtenstein, Switzerland), we initiated a retrospective case-finding study to investigate whether there were so far undetected human infections in Austria.</div></div><div><h3>Methods</h3><div>For this purpose, biobank samples from the Center for Virology in Vienna were selected based on available clinical characteristics consistent with possible neurological symptoms of human BoDV-1 infections to be screened for BoDV-1 RNA (859 cerebrospinal fluid samples) and anti-BoDV-1 IgG antibodies (366 corresponding serum samples).</div></div><div><h3>Results</h3><div>BoDV-1 RNA or confirmed anti-BoDV-1 IgG antibodies were not detected in any of the cerebrospinal fluid or serum samples, respectively.</div></div><div><h3>Conclusion</h3><div>Our result demonstrates that if human BoDV-1 infections occur in Austria, they must be very rare even in patients with neurological symptoms. Further research using samples with a more distinct geographical link to the circumscribed endemic rural region in Upper Austria, however, will be necessary to complement the preliminary finding of this study.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"134 ","pages":"Article 105806"},"PeriodicalIF":2.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144762354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0