Infection Genetics and Evolution最新文献

{"title":"Viral integration and fusion transcript characteristics of possibly high-risk HPV in cervical cancer","authors":"Hong Liu ,&nbsp;Ruiyi Xu ,&nbsp;Jinglan Chen ,&nbsp;Shuyan Wang ,&nbsp;Lingfang Wang ,&nbsp;Min Wu ,&nbsp;Hui Wang","doi":"10.1016/j.meegid.2025.105746","DOIUrl":"10.1016/j.meegid.2025.105746","url":null,"abstract":"<div><div>HPV34, HPV66, HPV73, and HPV82 are classified by the International Agency for Research on Cancer as possibly high-risk HPV (pHR-HPV) types. Despite their relatively lower pathogenicity, some cervical cancer (CC) patients have been found to be infected with pHR-HPV, though the underlying pathogenic characteristics remain unclear. Using viral integration detection and RNA sequencing in 8 pHR-HPV⁺ CC samples, we identified that the integration of pHR-HPV into the human genome and the formation of pHR-HPV-human fusion transcripts are critical events in cervical carcinogenesis. These events disrupt normal gene expression and favor the stable expression of oncogenes. Additionally, we discovered that pHR-HPV undergoes alternative splicing from the AGGTA motif. Despite their lower pathogenicity, pHR-HPV integration may represent a significant risk factor for CC development. Our findings underscore the importance of considering pHR-HPV infections in future HPV screening strategies and clinical management of cervical lesions, especially those associated with HPV integration.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"130 ","pages":"Article 105746"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection and identification of goose astrovirus in GuangDong Province, China","authors":"Hao Liu ,&nbsp;Ruyu Sun ,&nbsp;Yaning Wu,&nbsp;Jiayao Feng,&nbsp;Guiying Fan,&nbsp;Shuting Chen,&nbsp;Lixia Li","doi":"10.1016/j.meegid.2025.105745","DOIUrl":"10.1016/j.meegid.2025.105745","url":null,"abstract":"<div><div>In recent years, Goose Astrovirus (GAstV) has become a major pathogen in China, afflicting geese with significant clinical manifestations like gout and urate deposits in organs. Notably, similar symptoms have emerged in ducks. From 2023 to 2024, 126 liver samples were collected from ducks in Guangdong Province, where farms reported emaciation, paralysis, and deaths. Metagenomic analysis pinpointed GAstV as the primary pathogen, with a 43.65 % confirmed positive rate via qPCR. A new strain, GD2406, was identified, showing 97.5 %–98.8 % similarity to 30 GAstV-2 strains in GenBank. GD2406 displayed 98.3 % and 97.7 % identity with the duck strains HNNY0620 and SDTA, and 98.3 % and 98.4 % identity with highly virulent GAstV strains HNKF-1 and HNSQ-6. Phylogenetic analysis indicated a genetic closeness between GD2406 and the goose strain HB01. There were 13 amino acid mutations, mainly within ORF2, and a single mutation differing from HNKF-1 and HNSQ-6, hinting at significant pathogenic potential for both ducks and geese. This study is the first to report GAstV causing severe symptoms and mortality in Muscovy ducks in Guangdong Province, suggesting ducks could be key hosts and underscoring the risk of cross-species transmission.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"130 ","pages":"Article 105745"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological and molecular survey of Coxiella burnetii from the serum of patients suspected of brucellosis in west of Iran","authors":"Saeed Motamedi ,&nbsp;Ehsan Rashidian ,&nbsp;Amin Jaydari ,&nbsp;Heidar Rahimi ,&nbsp;Peyman Khademi","doi":"10.1016/j.meegid.2025.105743","DOIUrl":"10.1016/j.meegid.2025.105743","url":null,"abstract":"<div><div>Q fever is a global bacterial disease that affects both humans and animals. The etiological agent of the disease is <em>Coxiella burnetii</em>. The aim of the investigation was to detect the existence of <em>C. burneti</em> in the serum samples of patients with symptoms of fever and suspicion of brucellosis in the west of Iran. In the current survey, 150 Wright agglutination assay were collected from health centers in Lorestan, Hamadan, Ilam, Kermanshah, and Kurdistan provinces in 2023. DNA was extracted from all these samples, which were taken from individuals suspected of having brucellosis. Then, a nested PCR reaction was applied to diagnose <em>C. burnetii</em> for the transposon gene <em>IS1111</em>. The <em>IS1111</em> gene replication assays, indicated that 3.3 % (95 % CI: 1.43 %–3.33 %) of the tested serum samples, were positive for <em>C. burnetii.</em> The highest prevalence of <em>C. burnetii</em> infection was found in the Kurdistan province at 6.67 % (95 % CI: 1.85 %–21.33 %). Additionally, a high affinity (ranging from 99 % to 100 %) was recognized among the <em>IS1111</em> gene sequenced in the present study and those from different area around the world. These results provide strong evidence that individuals with brucellosis in west of Iran may also have <em>C. burnetii</em> co-infection with coxiellosis. Therefore, it is important to consider Q fever as a co-infection in patients suspected of brucellosis.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"130 ","pages":"Article 105743"},"PeriodicalIF":2.6,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing microbial ecology and antibiotic resistance genes in river sediments","authors":"Seyed Mehrdad Mirsalami ,&nbsp;Mahsa Mirsalami","doi":"10.1016/j.meegid.2025.105738","DOIUrl":"10.1016/j.meegid.2025.105738","url":null,"abstract":"<div><div>Anthropogenic activities greatly affect the Karon River leading to deterioration of water quality. This investigation utilizes environmental genomic techniques to delineate microbial populations, examine functional genomics, and evaluate the occurrence of virulence determinants and antibiotic resistance genes (ARGs) in fluvial sediment. Taxonomic assessment identified that Firmicutes were the predominant phyla, with Bacillus being the most abundant genus across samples. Functional analysis revealed the metabolic capabilities of sediment-associated bacteria, linking them to biogeochemical processes and potential health impacts. The S2 samples exhibited the highest virulence factor genes, while the S3 samples had the most ARGs (30), highlighting concerns about pathogenicity. Analyzing ARGs provides critical insights into environmental data collected, such as water quality parameters (e.g., nutrient concentrations, pH) or pollution levels, prevalence, and distribution of these resistance factors within the sediment samples, helping to identify potential hotspots of antibiotic resistance in the Karon River ecosystem. The study identified similar operational taxonomic units (OTUs) across sampling sites at the phylogenetic level, indicating a consistent presence of certain microbial taxa. However, the lack of variation in functional classification suggests that while these taxa may be present, they are not exhibiting significant differences in metabolic capabilities or functional roles. These findings emphasize the significance of metagenomic methods in understanding microbial ecology and antibiotic resistance in aquatic environments, suggesting a need for further research into the restoration of microbial functions related to ARGs and virulence factors.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"130 ","pages":"Article 105738"},"PeriodicalIF":2.6,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA sequencing analysis in pediatric patients with influenza-associated acute necrotizing encephalopathy: Potential biomarkers for early diagnosis and therapy","authors":"Wei Wang ,&nbsp;Jiehua Chen ,&nbsp;Yanmin Bao ,&nbsp;Weike Ma ,&nbsp;Ying Xie ,&nbsp;Wenjian Wang ,&nbsp;Meng Li ,&nbsp;Kunling Shen","doi":"10.1016/j.meegid.2025.105734","DOIUrl":"10.1016/j.meegid.2025.105734","url":null,"abstract":"<div><div>Acute necrotizing encephalopathy (ANE) secondary to influenza infection is characterized by fulminant neurological deterioration and a high mortality rate. The underlying mechanisms remain unclear, and specific treatments are currently lacking. Therefore, understanding the pathogenesis and identifying diagnostic and therapeutic targets for influenza-induced ANE are crucial. Peripheral blood samples were collected from two groups: influenza-infected patients without ANE (mild) and influenza infection with ANE patients (severe). Differentially expressed genes (DEG) were identified through microRNA sequencing analysis, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The expression levels of the four specific miRNAs were validated using qRT-PCR. In the severe group, 24 genes were up-regulated, and 67 genes were down-regulated compared to the mild group. The expression levels of hsa-miR-1290, hsa-miR-4657, has-miR-1231, and hsa-miR-342-3p were validated by qRT-PCR, and the levels of has-miR-4657 and hsamiR- 342-3p showed significant differences between severe and mild groups. GO analysis demonstrated that the DEGs were predominantly involved in the positive regulation of cellular processes, intracellular anatomical structure, and protein binding. KEGG pathway analysis revealed that DEGs were mainly enriched in calcium signaling pathway and axon guidance. The down-regulated hsa-miR-4657 and hsa-miR-342-3p might be associated with the development of ANE in pediatric patients with influenza by regulation of calcium pathways and axon guidance.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"130 ","pages":"Article 105734"},"PeriodicalIF":2.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143694418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of the emergence of novel sub-genotype XIII.2.3 of Newcastle disease virus in chickens from selected regions of Bangladesh","authors":"Farah Zereen ,&nbsp;Md. Abdur Rahman ,&nbsp;Md. Golzar Hossain ,&nbsp;Jahangir Alam ,&nbsp;Masaru Shimada ,&nbsp;Md. Tanvir Rahman ,&nbsp;Sukumar Saha","doi":"10.1016/j.meegid.2025.105742","DOIUrl":"10.1016/j.meegid.2025.105742","url":null,"abstract":"<div><div>Newcastle disease (ND) is one of the most economically devastating infectious diseases impacting the poultry industry in Bangladesh. This study aimed to characterize the pathotype, genotype, evolutionary divergence, and mutations of circulating virulent Newcastle disease virus (NDV) in chickens from the Gazipur, Tangail, and Mymensingh districts of Bangladesh between October 2023 and December 2024. ND-suspected samples, including lung, trachea, and caecal tonsil tissues, were collected, processed, and inoculated into 10–12-day-old embryonated chicken eggs (ECEs) via the allantoic cavity. Allantoic fluids were harvested after 24 h of incubation, and virulent NDV was identified through RT-PCR targeting the fusion (F) gene using specific primers. Pathogenicity was assessed using the mean death time (MDT), intracerebral pathogenicity index (ICPI), and intravenous pathogenicity index (IVPI). The pathotype and genotype were confirmed by complete sequencing of the F gene and phylogenetic analysis. Further evolutionary divergence and mutations were analyzed using MEGA-11 software. RT-PCR yielded specific amplification of a 254-bp product indicative of virulent NDV. Pathogenicity indices—MDT (&lt;60 h), ICPI (&gt;1.5), and IVPI (&gt;1.70)—confirmed a velogenic strain. Complete F gene sequencing revealed an F-protein cleavage site motif of “RRQKRF,” while phylogenetic analysis classified the isolates as belonging to sub-genotype XIII.2.3 under genotype XIII. Evolutionary divergence (0.00–0.06) and mutations at neutralizing epitopes 1 and 2 (at the 74th and 170th amino acids, respectively) suggested moderate genetic diversity. This study represents the first report in Bangladesh identifying the emergence of the novel sub-genotype XIII.2.3 of genotype XIII NDV associated with chicken mortality in selected regions.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"130 ","pages":"Article 105742"},"PeriodicalIF":2.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143694416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ListPred: A predictive ML tool for virulence potential and disinfectant tolerance in Listeria monocytogenes","authors":"Alexander Gmeiner ,&nbsp;Mirena Ivanova ,&nbsp;Rolf Sommer Kaas ,&nbsp;Yinghua Xiao ,&nbsp;Saria Otani ,&nbsp;Pimlapas Leekitcharoenphon","doi":"10.1016/j.meegid.2025.105739","DOIUrl":"10.1016/j.meegid.2025.105739","url":null,"abstract":"<div><div>Despite current surveillance and sanitation strategies, foodborne pathogens continue to threaten the food industry and public health. Whole genome sequencing (WGS) has reached an unprecedented resolution to analyse and compare pathogenic bacterial isolates. The increased resolution significantly enhances the possibility of tracing transmission routes and contamination sources of foodborne pathogens. In addition, machine learning (ML) on WGS data has shown promising applications for predicting important microbial traits such as virulence, growth potential, and resistance to antimicrobials. Many regulatory agencies have already adapted WGS and ML methods. However, the food industry hasn't followed a similarly enthusiastic implementation. Some possible reasons for this might be the lack of computational resources and limited expertise to analyse WGS and ML data and interpret the results. Here, we present ListPred, a ML tool to analyse WGS data of <em>Listeria monocytogenes,</em> a very concerning foodborne pathogen. ListPred relies on genomic markers and pre-trained ML models from two previous studies, and it is able to predict two important bacterial traits, namely virulence potential and disinfectant tolerance. ListPred only requires limited computational resources and practically no bioinformatic expertise, which is essential for a broad application in the food industry.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"130 ","pages":"Article 105739"},"PeriodicalIF":2.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of blaVEB-3 and blaKPC-2 coexistence with a novel blaKPC-2 transposon in Klebsiella michiganensis","authors":"Yuxia Zhong ,&nbsp;Peibo Yuan ,&nbsp;Liting Dai ,&nbsp;Ling Yang ,&nbsp;Zhenbo Xu ,&nbsp;Dingqiang Chen","doi":"10.1016/j.meegid.2025.105740","DOIUrl":"10.1016/j.meegid.2025.105740","url":null,"abstract":"<div><h3>Background</h3><div><em>Klebsiella michiganensis</em>, an emerging opportunistic pathogen, poses public health risks due to its increasing multidrug resistance (MDR), especially to carbapenems.</div></div><div><h3>Case and method</h3><div>A 46-year-old man with pulmonary fibrosis was hospitalized in Guangzhou, China, for worsening pneumonia. A multidrug-resistant <em>K. michiganensis</em> strain (YK6) was isolated from his sputum before treatment. The strain was characterized using MALDI-TOF mass spectrometry, antimicrobial susceptibility testing (AST), and whole genome sequencing (WGS). Targeted therapy guided by AST successfully resolved the infection.</div></div><div><h3>Results</h3><div>The YK6 strain exhibited resistance to carbapenems, β-lactam/β-lactamase inhibitors, cephalosporins, aminoglycosides, and quinolones, except colistin and tigecycline. Genomic analysis revealed a 41.9-kb MDR island and an intact I-E CRISPR-Cas system on the chromosome, along with two plasmids: IncFIA/IncFII plasmid pYK6–1 carrying <em>bla</em>_KPC-2 and IncC plasmid pYK6–2 harboring <em>bla</em>_VEB-3. A novel <em>bla</em>_KPC-2-transposon in pYK6–1 was identified, consisting of a non-Tn<em>4401</em> element (NTE)-like structure (Tn<em>3</em>-IS<em>Kpn27</em>-<em>bla</em>_KPC-2-ΔIS<em>Kpn6</em>-<em>korC</em>) flanked by inversely oriented IS<em>Kpn19</em>-<em>tnpM-tnpR</em> elements and 31-bp inverted repeats never reported, a configuration did not reported previously. Furthermore, the <em>bla</em>_VEB-3 genetic environment in pYK6–2 featured a unique cassette: IS<em>26</em>-IS<em>6100</em>-<em>bla</em>_VEB-3-<em>tnp</em>-IS<em>As1</em>-<em>qacEΔ1</em>-<em>sul1</em>-IS<em>CR1</em>. An additional IS<em>As1</em> insertion between the <em>tnpF</em>-like integrase and <em>qacEΔ1</em> distinguishes it from similar <em>bla</em>_VEB-3-harboring cassettes. The <em>bla</em>_VEB-3 resistance region in pYK6–2 likely originated from homologous recombination mediated by IS<em>26</em> and <em>Tn5403</em>, which flank the gene cassette.</div></div><div><h3>Conclusions</h3><div>To our knowledge, this is the first report of concurrent <em>bla</em>_VEB-3 and <em>bla</em>_KPC-2 in <em>K. michiganensis</em>, along with a novel <em>bla</em>_KPC-2 transposon structure. These findings highlight the urgent need for enhanced surveillance of MDR <em>K. michiganensis</em> to prevent treatment failures.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"130 ","pages":"Article 105740"},"PeriodicalIF":2.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome sequencing-based characterization of Listeria monocytogenes isolated from cattle and pig slaughterhouses","authors":"Serim Hong ,&nbsp;Jin-San Moon ,&nbsp;Young Ju Lee ,&nbsp;Ha-Young Kim","doi":"10.1016/j.meegid.2025.105737","DOIUrl":"10.1016/j.meegid.2025.105737","url":null,"abstract":"<div><div><em>Listeria monocytogenes</em> is a foodborne pathogen that causes human listeriosis and may be transmitted to humans via the food chain, beginning at slaughter and extending through food production and consumption. In this study, we performed whole-genome sequencing (WGS) analysis to determine the genetic characteristics of <em>L</em>. <em>monocytogenes</em> from the carcasses and environments of cattle and pig slaughterhouses in Korea. In total, 50 <em>L. monocytogenes</em> isolates were collected from 46 cattle and 47 pig slaughterhouses nationwide from 2014 to 2022. They were classified into two lineages, 12 sublineages, 12 sequence types, 11 clonal complexes (CCs), and 15 core-genome multilocus sequence types. <em>L. monocytogenes</em> isolates were divided into two lineages: lineage I (serotypes 1/2b and 4b) and lineage II (serotypes 1/2a and 1/2c). The most frequent CCs were CC9 (46.0 %), followed by CC224 (16.0 %) and CC155 (14.0 %). Although all isolates exhibited highly conserved LIPI-1, 20.0 % and 2.0 % contained LIPI-3 or LIPI-4, respectively. Moreover, 96.0 % of the isolates had full-length <em>inlA</em>. Interestingly, 21 of the 23 CC9 isolates contained mutations in <em>inlA</em> resulting from premature stop codon (PMSC). The <em>mdrL</em> and <em>Listeria</em> genomic island-2 (LGI-2) were identified in all <em>L</em>. <em>monocytogenes</em> isolates, whereas LGI-3 was identified in 32.0 % of the isolates. The <em>L</em>. <em>monocytogenes</em> isolates contained various antimicrobial resistance genes, moreover, the plasmid-borne resistance genes <em>tetM</em> and <em>mprF</em> were also identified in 34.0 % and 100 % of the isolates, respectively. Twenty-four isolates (48.0 %) harbored one or two plasmids (pLM33, DOp1, pLGUG1, and pLM5578), and 29 isolates (58.0 %) harbored at least one insertion sequence, composite transposon, and integrative conjugative element. Four isolates showed two CRISPR-Cas types I<img>B and II-A. In addition, phage sequences associated with the spacer constituting the CRISPR array were identified in 26 <em>Listeria</em> phages from 14 <em>L. monocytogenes</em> isolates. The genetic composition of <em>L</em>. <em>monocytogenes</em> was conserved in a collinearity relationship between each of the five <em>L</em>. <em>monocytogenes</em> isolates from the cattle and pig slaughterhouses. These findings suggest that <em>L</em>. <em>monocytogenes</em> isolated from cattle and pig slaughterhouses have the ability to cause human disease and exhibit virulent characteristics.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"130 ","pages":"Article 105737"},"PeriodicalIF":2.6,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of third-generation cephalosporin-resistant clinical isolate of Salmonella Bareilly ST203 harbouring plasmid-mediated AmpC β-lactamase CMY6 from India: Genome characteristics and transmissibility","authors":"Subhasree Roy ,&nbsp;Agniva Majumdar ,&nbsp;Souvik Nandy ,&nbsp;Juhi Pal ,&nbsp;Balaji Veeraraghavan ,&nbsp;Kamini Walia ,&nbsp;Shanta Dutta","doi":"10.1016/j.meegid.2025.105736","DOIUrl":"10.1016/j.meegid.2025.105736","url":null,"abstract":"<div><div>Non-typhoidal Salmonella (NTS) infections are a major public health concern in India because of inadequate knowledge of antimicrobial resistance, limiting therapeutic options. The study aimed to characterize and analyse the genome of a 3rd-generation cephalosporins (3GCs)-resistant clinical isolate of <em>Salmonella</em> Bareilly-harbouring plasmid-mediated AmpC (pAmpC) CMY-6. Identification, antibiotic susceptibility and Whole Genome Sequencing (WGS)-based analysis were performed. Transmissibility, replicon types of <em>bla</em>_CMY-6-harbouring plasmid were evaluated. <em>S.</em> Bareilly ST203 (Clonal-Complex 206.2) was isolated from clinical specimen of a paediatric patient and was found to be multidrug-resistant with resistance to 3rd generation cephalosporins, fluoroquinolone and aminoglycosides. WGS revealed pAmpC <em>bla</em>_CMY-6 on conjugative IncC plasmid (158,385 kb) which successfully transferred into the transconjugant with other resistance determinants (<em>bla</em>_TEM-1A, <em>armA, aac(6′)-Ib-cr, sul1</em>), showed higher MICs for 3GCs. Downstream regions of <em>bla</em>_CMY-6 include <em>blc</em> (lipocalin), <em>sugE</em> (efflux protein) and truncated <em>ecnR</em> (entericidin R) followed by other resistance genes. Presence of IS<em>Ecp1</em> in the genome facilitated the transfer of <em>bla</em>_CMY-6. Several efflux pump genes, two complete CRISPR arrays and intact phage sequences were also detected. Virulence factors associated with Salmonella Pathogenicity Islands SPI-1/SPI-2/SP-3 and their effectors indicated the virulence potential of this strain. To the best of our knowledge, genome of a 3GCs-resistant clinical isolate of <em>S.</em> Bareilly-harbouring pAmpC <em>bla</em>_CMY-6 was reported and analysed for the first time in this study. <em>S.</em> Bareilly was found to cause outbreaks in earlier reports but lower resistance was reported in this serovar compared to other NTS. As infections by NTS are concerning, early detection of such strains is of utmost importance.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"130 ","pages":"Article 105736"},"PeriodicalIF":2.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}