Infection Genetics and Evolution最新文献

{"title":"Experimental evolution of an RNA virus in Caenorhabditis elegans","authors":"Victoria G. Castiglioni ,&nbsp;María J. Olmo-Uceda ,&nbsp;Susana Martín ,&nbsp;Marie-Anne Félix ,&nbsp;Rubén González ,&nbsp;Santiago F. Elena","doi":"10.1016/j.meegid.2024.105623","DOIUrl":"10.1016/j.meegid.2024.105623","url":null,"abstract":"<div><p>The discovery of Orsay virus (OrV), the first virus infecting wild populations of <em>Caenorhabditis elegans</em>, has boosted studies of viral immunity pathways in this nematode. Considering the many advantages that <em>C. elegans</em> offers for fundamental research in host-pathogen interactions, this pathosystem has high potential to become a model system for experimental virus evolution studies. However, the evolutionary constraints – <em>i.e</em>, the balance between genetic variation, selection, drift and historical contingency- operating in this pathosystem have barely been explored. Here we describe for the first time an evolution experiment of two different OrV strains in <em>C. elegans</em>. Comparison of the two ancestral strains showed differences in infectivity and sequence, and highlighted the importance of consistently normalize viral inocula for meaningful comparisons among strains. After 10 serial passages of evolution, we report slight changes in infectivity and non-synonymous mutations fixed in the evolved viral populations. In addition, we observed numerous minor variants emerging in the viral population. These minor variants were not randomly distributed along the genome but concentrated in polymorphic genomic regions. Overall, our work established the grounds for future experimental virus evolution studies using <em>Caenorhabditis</em> nematodes.</p></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"123 ","pages":"Article 105623"},"PeriodicalIF":2.6,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567134824000741/pdfft?md5=c6e70c067014f412de4de57be5bfd483&pid=1-s2.0-S1567134824000741-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatitis A virus subtype IB outbreak among MSM in Hungary with a link to a frozen berry source","authors":"Ágnes Dencs ,&nbsp;Andrea Hettmann ,&nbsp;Erzsébet Barcsay ,&nbsp;Erzsébet Rusvai ,&nbsp;Emese Kozma ,&nbsp;Mária Takács","doi":"10.1016/j.meegid.2024.105622","DOIUrl":"10.1016/j.meegid.2024.105622","url":null,"abstract":"<div><p>Men who have sex with men (MSM) are at high risk of acquiring hepatitis A virus (HAV) and in recent years several HAV outbreaks mostly affecting MSM have been described. These outbreaks were caused by subtype IA strains circulating in this high-risk population. After years of low incidence, an outbreak among MSM in Hungary caused a significant increase in reported HAV infections in 2022.</p><p>Samples from 224 HAV IgM-positive patients diagnosed in 2022 were tested for HAV RNA and positive samples were genotyped by sequencing. In 171 patients a unique subtype IB virus was detected with 99.8–100% sequence identity in the VP1/P2A junction. It was distinct from previously published strains, but most closely related to an Egyptian isolate. Sequence analysis revealed one dominant and three minor variants based on VP1/P2A. Whole genome sequencing revealed limited variation among these variants, suggesting a recent common origin. Epidemiological data indicated that sexual transmission was driving the outbreak for most of the year, suggested by the high male to female ratio and the large number of coinfections with HIV and other sexually transmitted infections among the patients. The outbreak was also associated with a restaurant cluster, in which one of the variants was detected and frozen berries were implicated as the source of infections. The outbreak strain was also detected in other countries around Europe and remained frequently detectable in Hungary in 2023.</p><p>This study provides insights into the molecular and epidemiological characteristics of the described HAV outbreak. The results show that sequencing is not only useful in connecting cases to an outbreak, but also helps to clarify the relatedness of detected variants. Prevention strategies focusing on vulnerable communities may reduce the burden of HAV infections in the future.</p></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"123 ","pages":"Article 105622"},"PeriodicalIF":2.6,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S156713482400073X/pdfft?md5=8e2480defbdf74b54464b5c2ba77a316&pid=1-s2.0-S156713482400073X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete genome characterization and phylogenetic analysis of a novel polyomavirus detected in Eurasian beavers (Castor fiber)","authors":"András Surján,&nbsp;Balázs Harrach,&nbsp;Márton Z. Vidovszky","doi":"10.1016/j.meegid.2024.105620","DOIUrl":"10.1016/j.meegid.2024.105620","url":null,"abstract":"<div><p>The Eurasian beaver (<em>Castor fiber</em>), native to Hungary, faced local extinction in 1865 and was successfully reintroduced between mid-1980s and 2008. Despite screening programs focusing on animal health during reintroduction in other countries, information about viruses in the Hungarian beaver population remains limited. Polyomaviruses (PyVs) have been identified in various rodents, and have been detected just recently in beavers by us. In this paper we present the full genome analysis of the first PyV detected in Eurasian beaver. The novel PyV was discovered in the kidney tissues of two specimens. The genome is 5244 bp, and contains four genes. Small T-antigen (STAg) and alternative large T ORF (ALTO) genes are directly fused together forming the middle T-antigen (MTAg). VP3 is absent from the genome. Its large T-antigen (LTAg) coding sequence exhibited over 15% genetic divergence from known PyVs, supporting its classification into a new species within the genus <em>Alphapolyomavirus</em>, suggesting to be named <em>Alphapolyomavirus castoris</em>. Phylogenetic analysis, based on the LTAg gene showed, that the beaver PyV forms a distinct clade with primate PyVs within the genus <em>Alphapolyomavirus</em>, separate from other rodent PyVs. Phylogenetic study of the VP1 gene however showed this virus to belong in a distinct clade with the same primate PyVs, and additionally PyVs from rodents and a myocastor, which suggest host virus co-evolution. The virus detection of the euthanized beavers suggests an apathogenic persistent infections. The aquatic lifestyle of beavers may influence virus transmission, warranting further exploration of undiscovered viruses in beavers.</p></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"123 ","pages":"Article 105620"},"PeriodicalIF":3.2,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567134824000716/pdfft?md5=6ae538405c74fe5562623a7fced72c5d&pid=1-s2.0-S1567134824000716-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141321962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GII.6 norovirus major capsid protein VP1 derived from distinct clusters induce cross-blocking effects","authors":"Jie Ma,&nbsp;Jinjin Liu,&nbsp;Chaohong Fu,&nbsp;Yuqi Huo","doi":"10.1016/j.meegid.2024.105617","DOIUrl":"10.1016/j.meegid.2024.105617","url":null,"abstract":"<div><p>Unlike pandemic GII.4 norovirus, GII.6 norovirus shows limited sequence variation in its major capsid protein VP1. In this study, we investigated the VP1 expression profiles, binding abilities, and cross-blocking effects of three GII.6 norovirus strains derived from three distinct variants. Norovirus VP1 was expressed using a recombinant baculovirus expression system and characterized by transmission electron microscopy, mass spectrometry, salivary histo-blood group antigen (HBGA)-virus like particles (VLPs) binding and binding blockade assays. Mass spectrometry revealed the expected molecular weight (MW) of full-length proteins and degraded or cleaved fragments of all three VP1 proteins. Peptide mapping showed loss of 2 and 3 amino acids from the N- and C-terminus, respectively. Further, the co-expression of VP1 and VP2 proteins did not lead to extra fragmentation during mass spectrometry. Salivary HBGA-VLP binding assay revealed similar binding patterns of the three GII.6 VP1 proteins. Salivary HBGA-VLP binding blockade assay induced cross-blocking effects. Our results demonstrate similar binding abilities against salivary HBGAs and specific cross-blocking effects for GII.6 norovirus strains derived from distinct variants, suggesting that fewer GII.6 strains from different evolutionary variants are needed for the development of norovirus vaccines.</p></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"122 ","pages":"Article 105617"},"PeriodicalIF":3.2,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567134824000686/pdfft?md5=a20e6ff365a060e85aae767bd8ccfa1f&pid=1-s2.0-S1567134824000686-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141302176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiology of hepatitis B virus (HBV) in Ethiopia: A review article","authors":"Ayenew Assefa ,&nbsp;Molla Getie ,&nbsp;Birhanu Getie ,&nbsp;Takilosimeneh Yazie ,&nbsp;Aklesya Enkobahry","doi":"10.1016/j.meegid.2024.105618","DOIUrl":"10.1016/j.meegid.2024.105618","url":null,"abstract":"<div><p>Hepatitis B virus (HBV) belongs to the family Hepadnaviridae and is the smallest human DNA virus, with a genome that is only 3200 nucleotides long. The absence of proofreading function in HBV reverse transcriptase provides a wide range of genetic variants for targeted outgrowth at different stages of infection. A number of sub genotypes and ten HBV genotypes (A through J) have been identified through analyses of the divergence of HBV genomic sequences. Numerous clinical outcomes, including the emergence of chronicity, the course of the disease, the effectiveness of treatment, and the response to vaccination, have been related to differences in genotype between HBV isolates. There are just seven studies that have been done in Ethiopia that examine the molecular epidemiology of HBV. Moreover, these studies haven't been compiled and reviewed yet. In this review, we looked at the genetic diversity and molecular epidemiology of HBV, the relationship between HBV genotypes and clinical outcomes, the immunopathogenesis of HBV, and finally the molecular epidemiology of HBV in Ethiopia. PubMed, Embase, and Google Scholar search engines were used to find relevant articles for the review. By using HBV genotyping, clinicians can better tailor vaccination decisions and antiviral therapy for patients with chronic hepatitis B who are more likely to experience the disease's progression.</p></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"122 ","pages":"Article 105618"},"PeriodicalIF":3.2,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567134824000698/pdfft?md5=166804aa463fb39667c6d7eb6687bea7&pid=1-s2.0-S1567134824000698-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141302129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular characterization of Burkholderia mallei strains isolated from horses in Mongolia","authors":"Yoshiki Ichikawa ,&nbsp;Liushiqi Borjigin ,&nbsp;Batchuluun Enkhtuul ,&nbsp;Ochirbat Khurtsbaatar ,&nbsp;Keisuke Aoshima ,&nbsp;Atsushi Kobayashi ,&nbsp;Vanaabaatar Batbaatar ,&nbsp;Takashi Kimura","doi":"10.1016/j.meegid.2024.105616","DOIUrl":"10.1016/j.meegid.2024.105616","url":null,"abstract":"<div><p>Glanders, a highly contagious and often fatal disease affecting equids, is caused by <em>Burkholderia mallei</em>. Although sporadic cases of equine glanders have recently been documented in Mongolia, genome sequencing and molecular studies of the bacteria within this region are lacking. This study provided the first molecular characterization of <em>B. mallei</em> isolated from four native Mongolian horses from two different provinces in 2019 and 2022 by applying whole-genome sequencing with two SNP types (previously developed genotyping with 15 SNP markers that provide global coverage of the <em>B. mallei</em> population and the core genome coding SNP typing developed in this study). The Mongolian isolates were located within the L3B1 cluster, which was previously associated with the V-120 strain from Russia. Within the L3B1 cluster shared by neighboring countries, they were in a unique subbranch. In this study, specific SNP markers unique to the Mongolian strains were identified to track these strains using a high-resolution melting analysis (HRMA). This study revealed the unique phylogenetic background of Mongolian strains isolated from the eastern part of Mongolia. HRMA specific to the Mongolian subbranch may contribute to the molecular epidemiological monitoring of glanders in Mongolia and surrounding countries.</p></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"123 ","pages":"Article 105616"},"PeriodicalIF":3.2,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567134824000674/pdfft?md5=14809ce6db554fa9a4d281a2455cd70f&pid=1-s2.0-S1567134824000674-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141297318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial introduction: Special issue on immunology, epidemiology, prevention, diagnosis, and treatment of pediatric infectious diseases and neglected tropical diseases","authors":"Ran Wang ,&nbsp;Qingjie Xue ,&nbsp;Lingyun Guo","doi":"10.1016/j.meegid.2024.105615","DOIUrl":"10.1016/j.meegid.2024.105615","url":null,"abstract":"","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"122 ","pages":"Article 105615"},"PeriodicalIF":3.2,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567134824000662/pdfft?md5=302fccff68d4056bc8abcaf6a2784a56&pid=1-s2.0-S1567134824000662-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141288967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete sequencing of the Cryptosporidium suis gp60 gene reveals a novel type of tandem repeats—Implications for surveillance","authors":"Marianne Lebbad ,&nbsp;Jana Grüttner ,&nbsp;Jessica Beser ,&nbsp;Victor Lizana ,&nbsp;Maria Auxiliadora Dea-Ayuela ,&nbsp;Marianne Oropeza-Moe ,&nbsp;David Carmena ,&nbsp;Christen Rune Stensvold","doi":"10.1016/j.meegid.2024.105614","DOIUrl":"10.1016/j.meegid.2024.105614","url":null,"abstract":"<div><p>Cryptosporidiosis is an infectious enteric disease caused by species (some of them zoonotic) of the genus <em>Cryptosporidium</em> that in many countries are under surveillance. Typing assays critical to the surveillance of cryptosporidiosis typically involve characterization of <em>Cryptosporidium</em> glycoprotein 60 genes (<em>gp60</em>). Here, we characterized the <em>gp60</em> of <em>Cryptosporidium suis</em> from two samples—a human and a porcine faecal sample—based on which a preliminary typing scheme was developed. A conspicuous feature of the <em>C. suis gp60</em> was a novel type of tandem repeats located in the 5′ end of the gene and that took up 777/1635 bp (48%) of the gene. The <em>C. suis gp60</em> lacked the classical poly-serine repeats (TCA/TCG/TCT), which is usually subject to major genetic variation, and the length of the tandem repeat made a typing assay incorporating this region based on Sanger sequencing practically unfeasible. We therefore designed a typing assay based on the post-repeat region only and applied it to <em>C. suis</em>-positive samples from suid hosts from Norway, Denmark, and Spain. We were able to distinguish three different subtypes; XXVa-1, XXVa-2, and XXVa-3. Subtype XXVa-1 had a wider geographic distribution than the other subtypes and was also observed in the human sample. We think that the present data will inform future strategies to develop a <em>C. suis</em> typing assay that could be even more informative by including a greater part of the gene, including the tandem repeat region, e.g.<em>,</em> by the use of long-read next-generation sequencing.</p></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"122 ","pages":"Article 105614"},"PeriodicalIF":3.2,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567134824000650/pdfft?md5=7636cd0b5e8d1b3d6aa4054431d5ff3c&pid=1-s2.0-S1567134824000650-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141285389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Illuminating the pathogenic role of SARS-CoV-2: Insights into competing endogenous RNAs (ceRNAs) regulatory networks","authors":"Mahsa Aghajani Mir","doi":"10.1016/j.meegid.2024.105613","DOIUrl":"10.1016/j.meegid.2024.105613","url":null,"abstract":"<div><p>The appearance of SARS-CoV-2 in 2019 triggered a significant economic and health crisis worldwide, with heterogeneous molecular mechanisms that contribute to its development are not yet fully understood. Although substantial progress has been made in elucidating the mechanisms behind SARS-CoV-2 infection and therapy, it continues to rank among the top three global causes of mortality due to infectious illnesses. Non-coding RNAs (ncRNAs), being integral components across nearly all biological processes, demonstrate effective importance in viral pathogenesis. Regarding viral infections, ncRNAs have demonstrated their ability to modulate host reactions, viral replication, and host-pathogen interactions. However, the complex interactions of different types of ncRNAs in the progression of COVID-19 remains understudied. In recent years, a novel mechanism of post-transcriptional gene regulation known as “competing endogenous RNA (ceRNA)” has been proposed. Long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and viral ncRNAs function as ceRNAs, influencing the expression of associated genes by sequestering shared microRNAs. Recent research on SARS-CoV-2 has revealed that disruptions in specific ceRNA regulatory networks (ceRNETs) contribute to the abnormal expression of key infection-related genes and the establishment of distinctive infection characteristics. These findings present new opportunities to delve deeper into the underlying mechanisms of SARS-CoV-2 pathogenesis, offering potential biomarkers and therapeutic targets. This progress paves the way for a more comprehensive understanding of ceRNETs, shedding light on the intricate mechanisms involved. Further exploration of these mechanisms holds promise for enhancing our ability to prevent viral infections and develop effective antiviral treatments.</p></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"122 ","pages":"Article 105613"},"PeriodicalIF":3.2,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567134824000649/pdfft?md5=1d8e6800c2d9d3315b625c0fe05714b5&pid=1-s2.0-S1567134824000649-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141285390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fifty years after the first identification of Toscana virus in Italy: Genomic characterization of viral isolates within lineage A and aminoacidic markers of evolution","authors":"Giulia Marsili ,&nbsp;Carlo Pallotto ,&nbsp;Claudia Fortuna ,&nbsp;Antonello Amendola ,&nbsp;Cristiano Fiorentini ,&nbsp;Sara Esperti ,&nbsp;Pierluigi Blanc ,&nbsp;Lorenzo Roberto Suardi ,&nbsp;Venturi Giulietta ,&nbsp;Claudio Argentini","doi":"10.1016/j.meegid.2024.105601","DOIUrl":"10.1016/j.meegid.2024.105601","url":null,"abstract":"<div><p>Toscana Virus (TosV) was firstly isolated from phlebotomine in our Institute about fifty years ago. Later, in 1984–1985, TosV infection, although asymptomatic in most cases, was shown to cause disease in humans, mainly fever and meningitis. By means of genetic analysis of part of M segment, we describe 3 new viral isolates obtained directly from cerebrospinal fluid or sera samples of patients diagnosed with TosV infection in July 2020 in Tuscany region. Phylogenesis was used to propose the clustering of TosV lineage A strains in 3 main groups, whereas deep mutational analysis based on 12 amino acid positions, allowed the identification of 9 putative strains. We discuss deep mutational analysis as a method to identify molecular signature of host adaptation and/or pathogenesis.</p></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"122 ","pages":"Article 105601"},"PeriodicalIF":3.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567134824000522/pdfft?md5=b07396c628b7957211652013b82c9769&pid=1-s2.0-S1567134824000522-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141236354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}