{"title":"首次报道在孟加拉国选定地区的鸡中出现新型新城疫病毒XIII.2.3亚基因型。","authors":"Farah Zereen , Md. Abdur Rahman , Md. Golzar Hossain , Jahangir Alam , Masaru Shimada , Md. Tanvir Rahman , Sukumar Saha","doi":"10.1016/j.meegid.2025.105742","DOIUrl":null,"url":null,"abstract":"<div><div>Newcastle disease (ND) is one of the most economically devastating infectious diseases impacting the poultry industry in Bangladesh. This study aimed to characterize the pathotype, genotype, evolutionary divergence, and mutations of circulating virulent Newcastle disease virus (NDV) in chickens from the Gazipur, Tangail, and Mymensingh districts of Bangladesh between October 2023 and December 2024. ND-suspected samples, including lung, trachea, and caecal tonsil tissues, were collected, processed, and inoculated into 10–12-day-old embryonated chicken eggs (ECEs) via the allantoic cavity. Allantoic fluids were harvested after 24 h of incubation, and virulent NDV was identified through RT-PCR targeting the fusion (F) gene using specific primers. Pathogenicity was assessed using the mean death time (MDT), intracerebral pathogenicity index (ICPI), and intravenous pathogenicity index (IVPI). The pathotype and genotype were confirmed by complete sequencing of the F gene and phylogenetic analysis. Further evolutionary divergence and mutations were analyzed using MEGA-11 software. RT-PCR yielded specific amplification of a 254-bp product indicative of virulent NDV. Pathogenicity indices—MDT (<60 h), ICPI (>1.5), and IVPI (>1.70)—confirmed a velogenic strain. Complete F gene sequencing revealed an F-protein cleavage site motif of “RRQKRF,” while phylogenetic analysis classified the isolates as belonging to sub-genotype XIII.2.3 under genotype XIII. Evolutionary divergence (0.00–0.06) and mutations at neutralizing epitopes 1 and 2 (at the 74th and 170th amino acids, respectively) suggested moderate genetic diversity. This study represents the first report in Bangladesh identifying the emergence of the novel sub-genotype XIII.2.3 of genotype XIII NDV associated with chicken mortality in selected regions.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"130 ","pages":"Article 105742"},"PeriodicalIF":2.6000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"First report of the emergence of novel sub-genotype XIII.2.3 of Newcastle disease virus in chickens from selected regions of Bangladesh\",\"authors\":\"Farah Zereen , Md. Abdur Rahman , Md. Golzar Hossain , Jahangir Alam , Masaru Shimada , Md. Tanvir Rahman , Sukumar Saha\",\"doi\":\"10.1016/j.meegid.2025.105742\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Newcastle disease (ND) is one of the most economically devastating infectious diseases impacting the poultry industry in Bangladesh. This study aimed to characterize the pathotype, genotype, evolutionary divergence, and mutations of circulating virulent Newcastle disease virus (NDV) in chickens from the Gazipur, Tangail, and Mymensingh districts of Bangladesh between October 2023 and December 2024. ND-suspected samples, including lung, trachea, and caecal tonsil tissues, were collected, processed, and inoculated into 10–12-day-old embryonated chicken eggs (ECEs) via the allantoic cavity. Allantoic fluids were harvested after 24 h of incubation, and virulent NDV was identified through RT-PCR targeting the fusion (F) gene using specific primers. Pathogenicity was assessed using the mean death time (MDT), intracerebral pathogenicity index (ICPI), and intravenous pathogenicity index (IVPI). The pathotype and genotype were confirmed by complete sequencing of the F gene and phylogenetic analysis. Further evolutionary divergence and mutations were analyzed using MEGA-11 software. RT-PCR yielded specific amplification of a 254-bp product indicative of virulent NDV. Pathogenicity indices—MDT (<60 h), ICPI (>1.5), and IVPI (>1.70)—confirmed a velogenic strain. Complete F gene sequencing revealed an F-protein cleavage site motif of “RRQKRF,” while phylogenetic analysis classified the isolates as belonging to sub-genotype XIII.2.3 under genotype XIII. Evolutionary divergence (0.00–0.06) and mutations at neutralizing epitopes 1 and 2 (at the 74th and 170th amino acids, respectively) suggested moderate genetic diversity. This study represents the first report in Bangladesh identifying the emergence of the novel sub-genotype XIII.2.3 of genotype XIII NDV associated with chicken mortality in selected regions.</div></div>\",\"PeriodicalId\":54986,\"journal\":{\"name\":\"Infection Genetics and Evolution\",\"volume\":\"130 \",\"pages\":\"Article 105742\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-03-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Infection Genetics and Evolution\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1567134825000310\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infection Genetics and Evolution","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567134825000310","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
First report of the emergence of novel sub-genotype XIII.2.3 of Newcastle disease virus in chickens from selected regions of Bangladesh
Newcastle disease (ND) is one of the most economically devastating infectious diseases impacting the poultry industry in Bangladesh. This study aimed to characterize the pathotype, genotype, evolutionary divergence, and mutations of circulating virulent Newcastle disease virus (NDV) in chickens from the Gazipur, Tangail, and Mymensingh districts of Bangladesh between October 2023 and December 2024. ND-suspected samples, including lung, trachea, and caecal tonsil tissues, were collected, processed, and inoculated into 10–12-day-old embryonated chicken eggs (ECEs) via the allantoic cavity. Allantoic fluids were harvested after 24 h of incubation, and virulent NDV was identified through RT-PCR targeting the fusion (F) gene using specific primers. Pathogenicity was assessed using the mean death time (MDT), intracerebral pathogenicity index (ICPI), and intravenous pathogenicity index (IVPI). The pathotype and genotype were confirmed by complete sequencing of the F gene and phylogenetic analysis. Further evolutionary divergence and mutations were analyzed using MEGA-11 software. RT-PCR yielded specific amplification of a 254-bp product indicative of virulent NDV. Pathogenicity indices—MDT (<60 h), ICPI (>1.5), and IVPI (>1.70)—confirmed a velogenic strain. Complete F gene sequencing revealed an F-protein cleavage site motif of “RRQKRF,” while phylogenetic analysis classified the isolates as belonging to sub-genotype XIII.2.3 under genotype XIII. Evolutionary divergence (0.00–0.06) and mutations at neutralizing epitopes 1 and 2 (at the 74th and 170th amino acids, respectively) suggested moderate genetic diversity. This study represents the first report in Bangladesh identifying the emergence of the novel sub-genotype XIII.2.3 of genotype XIII NDV associated with chicken mortality in selected regions.
期刊介绍:
(aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID)
Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance.
However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors.
Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases.
Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .