印度携带质粒介导的AmpC β-内酰胺酶CMY6的第三代耐头孢菌素临床分离株Bareilly ST203的首次报道：基因组特征和传播率

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES

Infection Genetics and Evolution Pub Date : 2025-03-06 DOI:10.1016/j.meegid.2025.105736

Subhasree Roy , Agniva Majumdar , Souvik Nandy , Juhi Pal , Balaji Veeraraghavan , Kamini Walia , Shanta Dutta

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引用次数: 0

摘要

在印度，非伤寒沙门氏菌感染是一个主要的公共卫生问题，因为对抗菌素耐药性的认识不足，限制了治疗选择。本研究旨在对沙门氏菌质粒介导的AmpC (pAmpC) CMY-6的第3代头孢菌素耐药临床分离株进行基因组鉴定和分析。进行鉴定、抗生素敏感性和全基因组测序（WGS）分析。对携带blacmy -6质粒的传代率、复制子类型进行了评价。从一名儿科患者临床标本中分离出S. Bareilly ST203（克隆复合物206.2），发现其具有多重耐药，对第三代头孢菌素、氟喹诺酮类药物和氨基糖苷类药物具有耐药性。WGS发现pAmpC blaCMY-6在结合的IncC质粒（158,385 kb）上成功地与其他抗性决定因子（blacem - 1a, armA, aac(6')-Ib-cr, sul1）转偶联，对3gc表现出更高的mic。blaCMY-6的下游区域包括blc（脂钙蛋白）、糖基（外排蛋白）和截断的ecnR（肠毒素R），随后是其他抗性基因。基因组中ISEcp1的存在促进了blaCMY-6的转移。检测到几个外排泵基因、两个完整的CRISPR阵列和完整的噬菌体序列。与沙门氏菌致病性岛相关的毒力因子SPI-1/SPI-2/SP-3及其效应物显示了该菌株的毒力潜力。据我们所知，本研究首次报道并分析了含有S. bareilly - pAmpC blaCMY-6的3gc耐药临床分离株的基因组。在早期报告中发现S. Bareilly引起暴发，但与其他NTS相比，该血清型报告的耐药性较低。随着NTS感染的增加，尽早发现这种菌株是至关重要的。

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First report of third-generation cephalosporin-resistant clinical isolate of Salmonella Bareilly ST203 harbouring plasmid-mediated AmpC β-lactamase CMY6 from India: Genome characteristics and transmissibility

Non-typhoidal Salmonella (NTS) infections are a major public health concern in India because of inadequate knowledge of antimicrobial resistance, limiting therapeutic options. The study aimed to characterize and analyse the genome of a 3rd-generation cephalosporins (3GCs)-resistant clinical isolate of Salmonella Bareilly-harbouring plasmid-mediated AmpC (pAmpC) CMY-6. Identification, antibiotic susceptibility and Whole Genome Sequencing (WGS)-based analysis were performed. Transmissibility, replicon types of bla_CMY-6-harbouring plasmid were evaluated. S. Bareilly ST203 (Clonal-Complex 206.2) was isolated from clinical specimen of a paediatric patient and was found to be multidrug-resistant with resistance to 3rd generation cephalosporins, fluoroquinolone and aminoglycosides. WGS revealed pAmpC bla_CMY-6 on conjugative IncC plasmid (158,385 kb) which successfully transferred into the transconjugant with other resistance determinants (bla_TEM-1A, armA, aac(6′)-Ib-cr, sul1), showed higher MICs for 3GCs. Downstream regions of bla_CMY-6 include blc (lipocalin), sugE (efflux protein) and truncated ecnR (entericidin R) followed by other resistance genes. Presence of ISEcp1 in the genome facilitated the transfer of bla_CMY-6. Several efflux pump genes, two complete CRISPR arrays and intact phage sequences were also detected. Virulence factors associated with Salmonella Pathogenicity Islands SPI-1/SPI-2/SP-3 and their effectors indicated the virulence potential of this strain. To the best of our knowledge, genome of a 3GCs-resistant clinical isolate of S. Bareilly-harbouring pAmpC bla_CMY-6 was reported and analysed for the first time in this study. S. Bareilly was found to cause outbreaks in earlier reports but lower resistance was reported in this serovar compared to other NTS. As infections by NTS are concerning, early detection of such strains is of utmost importance.

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来源期刊

Infection Genetics and Evolution 医学-传染病学

CiteScore

8.40

自引率

0.00%

发文量

215

审稿时长

82 days

期刊介绍： (aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID) Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance. However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors. Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases. Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .