两种非鲍曼不动杆菌菌株的基因组见解，具有罕见的耐药机制，导致头孢地罗耐药。

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES

Infection Genetics and Evolution Pub Date : 2025-09-16 DOI:10.1016/j.meegid.2025.105820

Usman Akhtar , Samyar Moheb , Carol Davies-Sala , Joshua Gutierrez , Fernando Pasteran , Marisel R. Tuttobene , Tomás Subils , Chun Fu Cheng , Quentin Valle , Rajnikant Sharma , Marcelo E. Tolmasky , Gauri Rao , Robert A. Bonomo , German M. Traglia , María Soledad Ramírez

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引用次数: 0

摘要

不动杆菌物种抗菌素耐药性的出现构成了重大的临床挑战，特别是在非鲍曼原虫物种中，这在卫生保健环境中经常被忽视。在这项研究中，我们鉴定了两种临床分离的不动杆菌，AMA204和ama207，分别鉴定为朱尼假杆菌和溶血假杆菌，它们表现出罕见的耐药机制，能够在头孢地罗存在下存活，而不管它们的初始最低抑制浓度值如何。进行了全基因组测序和比较基因组分析，以调查与其抗性谱相关的遗传决定因素。药敏试验证实两株菌株均存在多药耐药，其中AMA204中含有blaOXA-58和blaNDM-1基因，AMA207中含有blaOXA-58和blaPER-2基因。系统基因组学分析揭示了地理上不同分离株的遗传相关性，提示可能的进化趋势和传播动态。此外，还分析了铁摄取系统，强调了导致头孢地罗耐药的潜在机制以及所列β-内酰胺酶的存在。这项研究强调了非鲍曼不动杆菌在抗菌素耐药性中的临床相关性，并强调需要持续监测和新的治疗策略来对抗这些新出现的威胁。

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Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistance

The emergence of antimicrobial resistance in Acinetobacter species poses a significant clinical challenge, particularly in non-baumannii species, which are often overlooked in healthcare settings. In this study, we characterized two Acinetobacter clinical isolates, AMA204 and AMA207—identified as A. junii and A. haemolyticus, respectively—which exhibit uncommon resistance mechanisms that enable survival in the presence of cefiderocol, regardless of their initial minimum inhibitory concentration values. Whole-genome sequencing and comparative genomic analyses were performed to investigate the genetic determinants associated with their resistance profiles. Antimicrobial susceptibility testing confirmed multidrug resistance, with both isolates harboring key β-lactamase genes, including bla_OXA-58, and bla_NDM-1 in AMA204, and bla_OXA-58 and bla_PER-2 in AMA207. Phylogenomic analyses revealed genetic relatedness to geographically diverse isolates, suggesting possible evolutionary trends and transmission dynamics. Additionally, iron uptake systems were analysed, highlighting potential mechanisms contributing to cefiderocol resistance together with the presence of listed β-lactamase. This study underscores the clinical relevance of non-baumannii Acinetobacter species in antimicrobial resistance and emphasizes the need for continued surveillance and novel therapeutic strategies to combat these emerging threats.

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来源期刊

Infection Genetics and Evolution 医学-传染病学

CiteScore

8.40

自引率

0.00%

发文量

215

审稿时长

82 days

期刊介绍： (aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID) Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance. However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors. Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases. Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .