Genomic characterization of a clinical Enterocloster aldenensis strain: First report in Thailand

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES

Infection Genetics and Evolution Pub Date : 2025-07-16 DOI:10.1016/j.meegid.2025.105800

Thunchanok Yaikhan , Mingkwan Yingkajorn , Worawut Duangsi-ngoen , Ei Phway Thant , Nattarika Chaichana , Sirikan Suwannasin , Kamonnut Singkhamanan , Saranyou Churi , Komwit Surachat

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引用次数: 0

Abstract

This study presents the first comprehensive genome analysis of Enterocloster aldenensis in Thailand, an organism typically found in the gut but occasionally acting as an opportunistic pathogen. A scrotal tissue sample from a patient with suspected Fournier's gangrene was initially collected for Bacteroides surveillance in Southern Thailand, E. aldenensis PSUA25 was identified to Bacteroides thetaiotaomicron by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and later reclassified as E. aldenensis following whole-genome sequencing. Species confirmation via Average Nucleotide Identity analysis showed 97 % identity with the representative strain. Phylogenetic analysis using all available E. aldenensis genomes revealed that strain PSU25A is closely related to AM40-2 AC—an isolate from human feces in China (NCBI BioSample: SAMN11413088) selected for comparative analysis based on high genomic similarity.

Comparative analysis revealed shared antimicrobial resistance genes, including poxtA, vanYG, vanWI, and vanTG. Unique to PSU25A were two mobile genetic elements: a conjugative transposon (Tn6009 with tetM) and a phage-associated region, suggesting horizontal gene transfer.

This study emphasizes the need for accurate microbial identification, as misidentification can impact treatment decisions. Understanding the genomic traits of E. aldenensis from specific regions provides valuable insights into its pathogenic potential.

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临床aldenensis菌株的基因组特征：泰国首次报道

本研究首次对泰国aldenensis肠clocloster进行了全面的基因组分析，这种生物通常存在于肠道中，但偶尔也会作为机会性病原体。在泰国南部首次收集了一名疑似富尼耶坏疽患者的阴囊组织样本用于拟杆菌监测，通过基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）鉴定了E. aldenensis PSUA25为拟杆菌thetaiotaomicron，随后通过全基因组测序将其重新分类为E. aldenensis。物种鉴定通过平均核苷酸鉴定分析显示97%的同源性。系统发育分析显示，菌株PSU25A与中国人类粪便分离株AM40-2 ac （NCBI BioSample: SAMN11413088）亲缘关系密切，具有较高的基因组相似性。比较分析发现共有的耐药基因包括poxtA、vanYG、vanWI和vanTG。PSU25A特有的两个可移动遗传元件：一个共轭转座子（Tn6009与tetM）和一个噬菌体相关区域，表明基因水平转移。这项研究强调需要准确的微生物鉴定，因为错误鉴定会影响治疗决策。了解来自特定区域的aldenensis的基因组特征为其致病潜力提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infection Genetics and Evolution 医学-传染病学

CiteScore

8.40

自引率

0.00%

发文量

215

审稿时长

82 days

期刊介绍： (aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID) Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance. However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors. Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases. Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .