Journal of Genetics and Genomics最新文献_第2页

Characterization of zebrafish alas2 reporter for study on erythropoiesis, blood flow, and erythroid disease. 斑马鱼alas2报告基因的特性及其在红细胞生成、血流和红细胞疾病研究中的应用。

IF 6.6 2区生物学

Journal of Genetics and Genomics Pub Date : 2025-07-01 Epub Date: 2025-03-24 DOI: 10.1016/j.jgg.2025.03.009

Wenjuan Zhang, Shuang Wu, Yaxin Li, Mengye Cao, Biao Cao, Dantong Li, Kai Chen, Jian Zhao, Weijun Pan

引用次数: 0

Establishing a semi-homology-directed recombination method for precision gene integration in axolotls. 建立蝾螈半同源定向重组精确基因整合方法。

IF 6.6 2区生物学

Journal of Genetics and Genomics Pub Date : 2025-07-01 Epub Date: 2025-03-07 DOI: 10.1016/j.jgg.2025.03.001

Liqun Wang, Yan Hu, Yuanhui Qiu, Huiting Lin, Xiang Li, Sulei Fu, Yan-Yun Zeng, Maria Ghouse, Cheng Long, Yanmei Liu, Ji-Feng Fei

{"title":"Establishing a semi-homology-directed recombination method for precision gene integration in axolotls.","authors":"Liqun Wang, Yan Hu, Yuanhui Qiu, Huiting Lin, Xiang Li, Sulei Fu, Yan-Yun Zeng, Maria Ghouse, Cheng Long, Yanmei Liu, Ji-Feng Fei","doi":"10.1016/j.jgg.2025.03.001","DOIUrl":"10.1016/j.jgg.2025.03.001","url":null,"abstract":"The axolotl is broadly used in regenerative, developmental, and evolutionary biology research. Targeted gene knock-in is crucial for precision transgenesis, enabling disease modeling, visualization, tracking, and functional manipulation of specific cells or genes of interest (GOIs). Existing CRISPR/Cas9-mediated homology-independent method for gene knock-in often causes \"scars/indels\" at integration junctions. Here, we develop a CRISPR/Cas9-mediated semi-homology-directed recombination (HDR) knock-in method using a donor construct containing a single homology arm for the precise integration of GOIs. This semi-HDR approach achieves seamless single-end integration of the Cherry reporter gene and a large inducible Cre cassette into intronless genes like Sox2 and Neurod6 in axolotls, which are challenging to modify with the homology-independent method. Additionally, we integrate the inducible Cre cassette into intron-containing loci (e.g., Nkx2.2 and FoxA2) without introducing indels via semi-HDR. GOIs are properly expressed in F0 founders, with approximately 5%-10% showing precise integration confirmed by genotyping. Furthermore, using the Nkx2.2:CreERT2 line, we fate-map spinal cord p3 neural progenitor cells, revealing that Nkx2.2+ cells adopt different lineages in development and regeneration, preferentially generating motoneurons over oligodendrocytes during regeneration. Overall, this semi-HDR method balances efficiency and precision in the integration of GOIs, providing a valuable tool for generating knock-in axolotls and potentially extending to other species.","PeriodicalId":54825,"journal":{"name":"Journal of Genetics and Genomics","volume":" ","pages":"942-953"},"PeriodicalIF":6.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neutrophils disrupt the intestinal barrier via IL-22/TGF-β/Mmp9 axis in the zebrafish model of inflammatory bowel disease. 炎症性肠病斑马鱼模型中，中性粒细胞通过IL-22/TGF-β/Mmp9轴破坏肠道屏障。

IF 6.6 2区生物学

Journal of Genetics and Genomics Pub Date : 2025-07-01 Epub Date: 2025-04-25 DOI: 10.1016/j.jgg.2025.04.012

Peixian Huang, Yiqing Yang, Junwei Lian, Tao Yu, Gaofei Li, Yiyue Zhang

引用次数: 0

Regulation of tillering and panicle branching in rice and wheat. 调节水稻和小麦的分蘖和圆锥花序分枝。

IF 6.6 2区生物学

Journal of Genetics and Genomics Pub Date : 2025-07-01 Epub Date: 2024-12-14 DOI: 10.1016/j.jgg.2024.12.005

Ning Zhang, Yuhao Liu, Songtao Gui, Yonghong Wang

引用次数: 0

The lawc gene emerged de novo from conserved genomic elements and acquired a broad expression pattern in Drosophila. lawc基因从保守的基因组元件中重新出现，并在果蝇中获得了广泛的表达模式。

IF 6.6 2区生物学

Journal of Genetics and Genomics Pub Date : 2025-07-01 Epub Date: 2024-12-27 DOI: 10.1016/j.jgg.2024.12.014

Roman O Cherezov, Julia E Vorontsova, Elena E Kuvaeva, Angelina A Akishina, Ekaterina L Zavoloka, Olga B Simonova

{"title":"The lawc gene emerged de novo from conserved genomic elements and acquired a broad expression pattern in Drosophila.","authors":"Roman O Cherezov, Julia E Vorontsova, Elena E Kuvaeva, Angelina A Akishina, Ekaterina L Zavoloka, Olga B Simonova","doi":"10.1016/j.jgg.2024.12.014","DOIUrl":"10.1016/j.jgg.2024.12.014","url":null,"abstract":"It has recently become evident that the de novo emergence of genes is widespread and documented for a variety of organisms. De novo genes frequently emerge in proximity to existing genes, forming gene overlaps. Here, we present an analysis of the evolutionary history of a putative de novo gene, lawc, which overlaps with the conserved Trf2 gene, which encodes a general transcription factor in Drosophila melanogaster. We demonstrate that lawc emerged approximately 68 million years ago in the 5'-untranslated region (UTR) of Trf2 and displays an extensive spatiotemporal expression pattern. One of the most remarkable features of the lawc evolutionary history is that its emergence was facilitated by the engagement of Drosophilidae-specific short, highly conserved regions located in Trf2 introns. This represents a unique example of putative de novo gene birth involving conserved DNA regions localized in introns of conserved genes. The observed lawc expression pattern may be due to the overlap of lawc with the 5'-UTR of Trf2. This study not only enriches our understanding of gene evolution but also highlights the complex interplay between genetic conservation and innovation.","PeriodicalId":54825,"journal":{"name":"Journal of Genetics and Genomics","volume":" ","pages":"901-914"},"PeriodicalIF":6.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variation in the promoter of OsNPF7.1 contributes to nitrate uptake, remobilization, and grain yield in upland rice. OsNPF7.1启动子的变异有助于旱稻对硝酸盐的吸收、再动员和籽粒产量。

IF 6.6 2区生物学

Journal of Genetics and Genomics Pub Date : 2025-07-01 Epub Date: 2025-03-26 DOI: 10.1016/j.jgg.2025.03.010

Ming Yan, Huimin Feng, Mian Gu, Hanwei Mei, Lei Wang, Kai Xu, Shoujun Chen, Anning Zhang, Liguo Zhou, Xiaoyan Xu, Peiqing Fan, Liang Chen, Fangjun Feng, Guohua Xu, Lijun Luo, Hui Xia

引用次数: 0

Design strategies for enhanced sustainable green revolution productivity in rice. 提高水稻可持续绿色革命生产力的设计策略。

IF 6.6 2区生物学

Journal of Genetics and Genomics Pub Date : 2025-06-30 DOI: 10.1016/j.jgg.2025.06.004

Shuoxun Wang, Jie Hu, Wenzhen Song, Qiaoling Zhang, Chenchen Wu, Jiangyi Zhou, Lindong Yang, Yunzhe Wu, Yafeng Ye, Weishu Fan, Xiangdong Fu, Kun Wu

{"title":"Design strategies for enhanced sustainable green revolution productivity in rice.","authors":"Shuoxun Wang, Jie Hu, Wenzhen Song, Qiaoling Zhang, Chenchen Wu, Jiangyi Zhou, Lindong Yang, Yunzhe Wu, Yafeng Ye, Weishu Fan, Xiangdong Fu, Kun Wu","doi":"10.1016/j.jgg.2025.06.004","DOIUrl":"10.1016/j.jgg.2025.06.004","url":null,"abstract":"Modern agriculture relies heavily on resource-intensive and environmentally harmful inputs, while the increasing global population and decreasing arable land demand new strategies to improve sustainable productivity of cereal crops, particularly reducing inorganic nitrogen fertilizer use while simultaneously increasing photosynthesis and grain yield in rice. To improve rice productivity, it is essential to improve photosynthetic nitrogen assimilation and optimize the translocation of carbon and nitrogen products from source to sink tissues. In this review, we first summarize recent advances in the genetic basis for improving grain yield by enhancing photosynthetic carbon and nitrogen assimilation. We then discuss progress in modulating the source-sink relationships to achieve higher yield and improved harvest index. Finally, we explore the necessary optimizations for adapting rice to high-density planting. These advancements are driving the development of sustainable green revolution varieties through the rational design of multi-gene pyramids and artificial intelligence (AI)-driven protein engineering.","PeriodicalId":54825,"journal":{"name":"Journal of Genetics and Genomics","volume":" ","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144555825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cohesin variants and meiotic timing shape chromosome segregation accuracy. 内聚蛋白变异与减数分裂时形染色体分离精度。

IF 6.6 2区生物学

Journal of Genetics and Genomics Pub Date : 2025-06-27 DOI: 10.1016/j.jgg.2025.06.003

Yuanyuan Liu, Bohan Liu, Shuo Wang, Li Zhao, Qian Li, Feifei Qi, Ruoxi Wang, Jun Zhou, Jinmin Gao

{"title":"Cohesin variants and meiotic timing shape chromosome segregation accuracy.","authors":"Yuanyuan Liu, Bohan Liu, Shuo Wang, Li Zhao, Qian Li, Feifei Qi, Ruoxi Wang, Jun Zhou, Jinmin Gao","doi":"10.1016/j.jgg.2025.06.003","DOIUrl":"https://doi.org/10.1016/j.jgg.2025.06.003","url":null,"abstract":"The frequency of aneuploid gamete formation increases with maternal age, yet the effects of genetic variants on meiotic chromosome segregation accuracy during aging remain poorly understood. Using the multicellular organism Caenorhabditis elegans, we investigate the impact of mutations in the conserved cohesin complex on age-associated meiotic errors. Point mutations in the head domain of the cohesin component SMC-1, which alter local hydrophobicity, cause meiotic defects that vary with age. A severe mutation causes incomplete synapsis and defective crossover formation, and a minor one causes age-related diakinesis bivalent abnormalities. Notably, while the mild mutation causes defects only in aged worms, worms with the severe mutation exhibit significantly alleviated phenotypes with age. Genetic and cytological analyses suggest that this alleviation results from a slowed meiotic progression during early prophase, which restores impaired cohesin loading. These findings reveal that cohesin variants, meiotic progression speed during early prophase, and the overall duration of meiosis collectively shape the accuracy of meiotic chromosome segregation.","PeriodicalId":54825,"journal":{"name":"Journal of Genetics and Genomics","volume":" ","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144531185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanistic insights into fruit ripening: integrating phytohormones, transcription factors, and epigenetic modification. 水果成熟的机制：整合植物激素、转录因子和表观遗传修饰。

IF 6.6 2区生物学

Journal of Genetics and Genomics Pub Date : 2025-06-26 DOI: 10.1016/j.jgg.2025.06.001

Chengpeng Yang, Shiyu Ying, Beibei Tang, Chuying Yu, Yikui Wang, Mengbo Wu, Mingchun Liu

{"title":"The mechanistic insights into fruit ripening: integrating phytohormones, transcription factors, and epigenetic modification.","authors":"Chengpeng Yang, Shiyu Ying, Beibei Tang, Chuying Yu, Yikui Wang, Mengbo Wu, Mingchun Liu","doi":"10.1016/j.jgg.2025.06.001","DOIUrl":"https://doi.org/10.1016/j.jgg.2025.06.001","url":null,"abstract":"Fruit ripening is a complex developmental process tightly regulated by hormonal crosstalk, transcriptional networks, and epigenetic modifications, with striking divergence between climacteric and non-climacteric species. In climacteric fruits, such as tomatoes, apples, and bananas, ethylene acts as the master regulator, driving autocatalytic biosynthesis through ACS/ACO genes and activating hierarchical transcriptional cascades mediated by MADS-box (RIN), NAC (NOR), and ERF-family transcription factors. These pathways are amplified by epigenetic reprogramming, including DNA demethylation at ripening-related promoters and histone acetylation, which enhance chromatin accessibility to facilitate gene expression. Conversely, non-climacteric fruits like strawberries and grapes predominantly rely on abscisic acid (ABA) to coordinate ripening. Hormonal interplay-such as ethylene-ABA synergy in climacteric fruits systems-further fine-tunes ripening dynamics. Advances in CRISPR-based gene editing and epigenome engineering now enable precise manipulation of these pathways, offering transformative solutions to reduce postharvest losses, enhance nutritional quality, and improve climate resilience. This review integrates mechanistic insights across species, emphasizing opportunities to translate fundamental discoveries into sustainable agricultural innovations, from breeding nutrient-rich cultivars to optimizing postharvest technologies for global food security.","PeriodicalId":54825,"journal":{"name":"Journal of Genetics and Genomics","volume":" ","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144531186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The nucleoporin CPR5 and histone chaperone NAP1 form a complex to negatively modulate plant immunity. 核孔蛋白CPR5和组蛋白伴侣蛋白NAP1形成复合物负向调节植物免疫。

IF 6.6 2区生物学

Journal of Genetics and Genomics Pub Date : 2025-06-26 DOI: 10.1016/j.jgg.2025.06.002

Fenghui Xu, Xinyi Cai, Yi Yang, Yuehui Zhang, Weiyi Dou, Leiwen Pan, Shui Wang

引用次数: 0