New Microbiologica最新文献

{"title":"A case report of co-isolation of Lactobacillus delbrueckii and Candida albicans from blood cultures of an elderly woman with diabetes mellitus.","authors":"Fotis Panagopoulos, Dimitris Kounatidis, Gerasimos Socrates Christodoulatos, Orestis Zormpas, Ilektra Tzivaki, Tatiana Zachariadou, Nikolaos Adamidis, Natalia Vallianou","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of this study is to describe a rare case of dual isolation of Candida albicans and Lactobacillus delbrueckii from blood cultures of an 86-year-old female patient. The patient, an elderly individual, had a medical history remarkable for diabetes mellitus, which was currently being managed with an SGLT-2 inhibitor. The patient exhibited elevated fevers, which were attributed to candidemia and bacteremia caused by Lactobacillus delbrueckii. The patient's candidemia originated from a urinary tract infection. The isolation of Lactobacillus delbrueckii in three sets of blood cultures was linked to the regular consumption of yogurts, some of which were past their expiration date. The patient's bacteremia may be attributed to the ingestion of yogurts, which could have facilitated bacterial translocation from the gastrointestinal tract to the systemic circulation.</p>","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"48 2","pages":"200-203"},"PeriodicalIF":1.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145350085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of probable drug targets at the outer membrane of Yersinia enterocolitica.","authors":"Ahmed F Roumia, Kamal Mahmoud, Vianey Mendez-Trujillo, Daniel Gonzalez-Mendoza, Olivia Tzintzun-Camacho, Claudia Michel-Lopez, Ali Abdelmoteleb","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Yersinia enterocolitica is a food-borne bacterium related to a disease called yersiniosis (gastroenteritis) in animals and humans. It is widely distributed in nature and is controlled with various antibiotics, such as second- and third-generation cephalosporins, gentamicin and fluoroquinolones and ciprofloxacin. In addition to antibiotic resistance, these antibiotics have side effects in the host because of drug-host interaction. To overcome these problems, this study focused on finding putative drug targets in the pathogen Y. enterocolitica and identifying novel molecules to tackle the function of this pathogen. In the present study, two new drug targets were found in Y. enterocolitica. Apart from using antibiotics, chlorogenic acid, hesperidin, neohesperidin, and naringin had indicated good binding affinity with the recognized targets through molecular docking experiments. Accordingly, this investigation suggests that the newly-found drug targets may be a fertile field in the pharmaceutical community for finding inhibitors similar to chlorogenic acid, hesperidin, neohesperidin, and naringin, as well as designing new drug molecule formulations to tackle the foodborne illness caused by pathogen Y. enterocolitica.</p>","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"48 2","pages":"174-182"},"PeriodicalIF":1.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145350076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Game on for prevention: using gamification to assess and spread hand hygiene knowledge amongst healthcare workers.","authors":"Zeno Dalla Valle, Maria Elena Ales, Antonio Antonelli, Giulia Cattabianchi, Carlo Signorelli, Matteo Moro","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>hand hygiene is vital for preventing healthcare-associated infections, yet compliance among healthcare workers is low. To address this, we created an interactive online quiz using gamification to promote self-assessment and education.</p><p><strong>Methods: </strong>the quiz was emailed to healthcare workers and students at a teaching hospital in Milan, Italy. Responses from 28/09/2023 to 09/02/2024 were analyzed, comparing correct response rates and average scores across categories (physicians, professionals, assistants, students, and others).</p><p><strong>Results: </strong>the gamification strategy led to high engagement, with 1,487 quizzes analyzed (14.0% of invited participants). Only 59% correctly identified the recommended handwashing duration, and 33% of physicians mistakenly thought it exceeded two minutes. Just 30% knew the WHO-recommended hand hygiene technique includes six steps. Half of respondents were unaware that soap is advised for C. difficile cases, though healthcare professionals were twice as likely as physicians to answer correctly. Healthcare professionals and assistants achieved significantly higher scores than physicians.</p><p><strong>Conclusion: </strong>the findings highlighted a significant educational gap in hand hygiene knowledge among healthcare workers. However, strong engagement showed gamification's potential to address this gap. These results support combining gamification with other validated tools to improve compliance and knowledge.</p>","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"48 2","pages":"168-173"},"PeriodicalIF":1.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145350135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clavicular Fracture-Associated Candida parapsilosis Osteomyelitis: A Case Report and State-of-the-Art Perspective.","authors":"Marinella Cibelli, Luisa Frallonardo, Guido Giacomo, Fabiano Michele Mariani, Francesco Di Gennaro, Massimiliano Carrozzo, Giuseppe Solarino, Rossella Elia, Giuseppe Giudice, Michele Maruccia, Annalisa Saracino","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Candida parapsilosis is an infrequent opportunistic pathogen, responsible for approximately 7% of Candida osteomyelitis cases. While infections in immunocompetent individuals are rare, they present significant therapeutic challenges due to intrinsic resistance mechanisms and biofilm formation, requiring a multidisciplinary management approach. We report the case of a 27-year-old immunocompetent man who developed Candida parapsilosis osteomyelitis following surgical fixation of a clavicular fracture sustained in a road traffic accident. After multiple surgical interventions due to persistent infection, C. parapsilosis was identified in bone samples, along with Klebsiella pneumoniae. The patient underwent prolonged antifungal and antibiotic therapy, combined with surgical debridement and bone reconstruction using a microsurgical fibular flap. He remained clinically stable, with no signs of recurrence at 11-month follow-up. This case underscores the diagnostic and therapeutic challenges of Candida parapsilosis osteomyelitis, particularly in immunocompetent individuals, who are often not considered at risk for fungal infections. It highlights the importance of early clinical suspicion, prolonged microbiological cultures, and a multidisciplinary team approach integrating both surgical and medical management to optimize patient outcomes. Additionally, a review of the literature on C. parapsilosis osteomyelitis is provided.</p>","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"48 2","pages":"204-209"},"PeriodicalIF":1.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145350132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sexually Transmitted Infections: Global Trends, Diagnostic Advances, and Emerging Challenges.","authors":"Semih Esin, Laura Del Bono, Mauro Pistello","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Sexually transmitted infections (STIs) continue to pose a major public health challenge and have profound social, economic and health impacts. These infections, which are caused by a variety of bacteria, viruses and parasites, are primarily transmitted through sexual contact, but can also be passed from mother to child during childbirth or through contaminated needles and blood products. Despite advances in medicine and public health, the prevalence of STIs continues to rise, fueled by factors such as stigma, limited access to sexual health services, and evolving pathogens. This review will examine the global burden of STIs and highlight their prevalence and impact on different populations. It will discuss some of the diagnostic challenges that hinder the effective management and control of these infections. It will also discuss the wider public health implications, including the economic costs, and identify emerging challenges and potential strategies to mitigate their impact.</p>","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"48 2","pages":"113-136"},"PeriodicalIF":1.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145349929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caspofungin in the treatment of Candida auris candiduria in Hemato-oncological Patients: An observational retrospective cohort study.","authors":"Umran Elbahr, Amira Khairy, Farouq Dayyab, Clark Steven Delos Reyes, Jennie Pastrana, Chithra Vineeth, Ahmed Qaedi, Suha Hejres, Shruti Prem Sudha, Ozge Keskin, Shiv Pratap Singh Rana, Elias Fadel, Hakan Erdem, Oguz Resat Sipahi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Candiduria is a common problem especially in advanced stage oncology/hematology cases. Herein, we aimed to analyze the efficacy of caspofungin treatment in Candida auris isolated from urine culture. We conducted an observational retrospective study in a tertiary-care educational hemato-oncology hospital from October 2021 to November 2022. The patients hospitalized in our center and having at least two consecutive urine cultures that yielded C. auris with significant pyuria were included in the study. The effectiveness of caspofungin was evaluated based on clinical and microbiological outcomes. In total, 10 patients (two female, aged 55.3 ± 15) were included in the study. Four (40%) patients had urinary tract infections, and six (60%) patients had asymptomatic C. auris candiduria which required antifungal therapy. Nine cases had urinary instrumentation: two nephrostomy, one suprapubic catheter, and six urinary catheters. The 10 cases received a mean of 13.4 ± 3.5 days of caspofungin. Four of 10 cases (40%) had at least 1 negative culture between day 1 and the end of caspofungin treatment. Relapse and re-infection rates 30 days after the end of therapy in microbiologically successful cases were 3/4 and 2/4. Caspofungin resistance developed in three cases (30%) within 30 days after treatment. Overall day-30 mortality was 3/10 (30%) while overall success (microbiological success + no relapse/reinfection during 30 days follow up after end of therapy) was 10%. Caspofungin demonstrated limited efficacy in treating C. auris-related urinary tract infection/colonization in hospitalized cancer patients.</p>","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"48 2","pages":"155-159"},"PeriodicalIF":1.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145350046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of fast microbiology assays on clinical stewardship: a retrospective study.","authors":"Cosimo Racco, Alberico Parente, Blerta Kertusha, Marzia Tagliaferro, Erica Cavaleri, Riccardo Lubrano, Miriam Lichtner, Umberto Basile, Cosmo Del Borgo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Acute respiratory infections (ARIs) are among the most common infections, with variable etiology, affecting both community and hospital settings. Vulnerable populations include children, the elderly, and patients with chronic debilitating disease. Early identification of the causative pathogen can reduce inappropriate empirical antibiotic therapy, particularly in viral infection cases, which are the leading cause of ARIs, especially in outpatient settings. Rapid molecular diagnostic methods such as FilmArray Respiratory Panel (FA RP) enable the prompt identification of viral and bacterial pathogens. A retrospective observational study was conducted at Santa Maria Goretti Hospital in Latina, Italy, from January 1, 2022, to April 30, 2023. A total of 232 nasopharyngeal swabs from patients were analyzed using the BioFire FA RP. Eligible patients exhibited fever, cough, or other symptoms suggestive of respiratory infection. The test distribution shows that the Pediatric and Neonatology Units had the highest number of tests and a high proportion of clinically relevant outcomes. The findings underscore the effectiveness of the FA RP in swiftly identifying pathogens, significantly reducing diagnostic turnaround times, and minimizing unnecessary antibiotic prescriptions. The 38% rate of negative tests highlights the importance of appropriate test prescription based on clinical assessment.</p>","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"48 2","pages":"183-189"},"PeriodicalIF":1.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145349913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome sequencing of two multidrug-resistant carbapenemase-producing Serratia marcescens strains.","authors":"Xuesong Wang, Yunlong Li, Jing Yu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To understand the resistance phenotype and the carriage of resistance genes in carbapenem-resistant Serratia marcescens in our hospital. Two S. marcescens clinical isolates were analyzed retrospectively to determine their sensitivity to 27 antimicrobial agents using the BD Phoenix™ M50 System (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) and Kirby-Bauer disk diffusion method (Oxoid, Hampshire, UK). Whole-genome sequencing of both strains was performed using the Illumina platform. ABRicate software was used to predict the resistance genes and plasmid replicon types carried by the strains. Sequence type (ST) analysis was performed using multilocus sequence typing. A phylogenetic tree was constructed, and homology analysis was conducted by comparing the two strains with 106 blaKPC-2-carrying carbapenem-resistant S. marcescens isolates downloaded from the NCBI database. Two S. marcescens strains were isolated from the sputum samples of patients with lower respiratory tract infections after ICU surgery. Antimicrobial sensitivity testing revealed that both strains were resistant to antimicrobial agents, including β-lactams, sulfonamides, and carbapenems, whereas they were susceptible to tigecycline and ceftazidime/avibactam. In total, 12 resistance genes were predicted, with both WF0070 and WF0071 carrying the carbapenem resistance gene blaKPC-2. Among the remaining resistance genes, the aminoglycoside resistance genes were most commonly predicted, with five types identified. The two strains in this study and the 106 blaKPC-2-carrying carbapenem-resistant S. marcescens isolates downloaded from NCBI were divided into five evolutionary groups, and both strains in this experiment were categorized into clade E. Meanwhile, 18 STs were predicted, and both strains in this study belonged to ST366. In terms of phylogenetic relationships, these strains exhibited high homology with a strain isolated in France in 2018. The two S. marcescens strains in this study displayed high resistance to multiple antimicrobial agents, and both carried the carbapenem resistance gene blaKPC-2 along with several other resistance genes. The two strains displayed close phylogenetic relationships, and the possibility of nosocomial transmission cannot be dismissed.</p>","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"48 2","pages":"190-196"},"PeriodicalIF":1.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145349872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulmonary Cytomegalovirus replication in Immunocompetent Hosts: Harmless Bystander or Hidden Threat?","authors":"Sergio Venturini, Ingrid Reffo, Manuela Avolio, Rosamaria Tedeschi, Maria Teresa Bortolin, Giovanni Del Fabro, Astrid Callegari, Maurizio Tonizzo, Silvia Grazioli, Ada Zanier, Elena Garlatti Costa, Stefano Tavano, Umberto Zuccon","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We investigated CMV DNA pulmonary viral loads in adults in asymptomatic patients without classic immunosuppressive conditions undergoing bronchoalveolar lavage (BAL) for screening non-acute, non-infectious pulmonary diseases with a six-month follow-up. Over 31 months, 73 out of 352 BAL samples (20.7%) tested positive, with wide viral load variability (median 829 copies/mL, IQR 1457 copies/mL). None of the patients received antiviral treatment or developed CMV-related disease during follow-up. Nine patients died during follow-up. Patients presented a range of risk factors, and none was associated with higher VL. These findings suggest that CMV pulmonary behavior deserves further attention across various populations.</p>","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"48 2","pages":"197-199"},"PeriodicalIF":1.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145349942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eravacycline: Data Based on Real Evidence.","authors":"Simone Giuliano, Luca Martini, Francesca Prataviera, Chiara Moreal, Sarah Flammini, Giovanni Terrosu, Lucio De Cecchis, Davide Lazzarotto, George G Zhanel, Carlo Tascini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Eravacycline, a novel synthetic fluorocycline, exhibits broad-spectrum antimicrobial activity against multidrug-resistant (MDR) Gram-positive, Gram-negative, and anaerobic bacteria, making it a promising option for complicated intra-abdominal infections (cIAI). Engineered to overcome common tetracycline resistance mechanisms, eravacycline has demonstrated efficacy in vitro against MDR pathogens, including carbapenem-resistant Enterobacterales and Acinetobacter baumannii. Clinical trials (IGNITE1 and IGNITE4) established its noninferiority to carbapenems in treating cIAI, with comparable clinical cure rates but higher adverse gastrointestinal events. This retrospective study evaluates real-world eravacycline use in 13 hospitalized patients treated for complicated infections at a single center between April 2023 and August 2024. Data collected include patient demographics, infection characteristics, microbiological profiles, antibiotic regimens, and clinical outcomes. The clinical cure rate was 69.2%, with a mortality rate of 38.5%. Resistance mechanisms such as VIM-producing Klebsiella pneumoniae and Enterobacter cloacae and inadequate source control were prevalent among non-survivors. Most patients received combination therapy, primarily with beta-lactams. One suspected case of drug-induced hepatotoxicity was observed. Our findings align closely with previous case series, underscoring eravacycline's efficacy in MDR infections. However, limitations such as small sample size and retrospective design warrant further controlled studies to refine its role in complex infections beyond cIAI.</p>","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"48 2","pages":"137-146"},"PeriodicalIF":1.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145350048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}