New Microbiologica最新文献_第6页

Peritoneal dialysis-related peritonitis caused by Stephanoascus ciferrii: A Case Report. Stephanoascus ciferrii 引起的腹膜透析相关腹膜炎：病例报告。

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-07-01

Wenchen Guo, Ting Guo, Yaowen Wang, Wanxiang Li

{"title":"Peritoneal dialysis-related peritonitis caused by Stephanoascus ciferrii: A Case Report.","authors":"Wenchen Guo, Ting Guo, Yaowen Wang, Wanxiang Li","doi":"","DOIUrl":"","url":null,"abstract":"Stephanoascus ciferrii, a conditional pathogenic fungus prevalent in nature, is more frequently encountered in patients with compromised immunity. However, the literature rarely reports infections caused by Stephanoascus ciferrii in peritoneal dialysis patients. Here, we detail the case of a 66-year-old female suffering from renal failure who experienced catheter-related infection during peritoneal dialysis. Dialysate turbidity prompted the detection of Stephanoascus ciferrii in both peritoneal dialysate and tubes through microbiological cultures. Subsequent treatment involved antifungal drugs and a transition to hemodialysis, resulting in the disappearance of peritonitis symptoms and the patient's discharge. In recent years, fungal infections, particularly dialysis-related infections, are on the rise. This marks the first reported case of catheter-related peritonitis infection caused by Stephanoascus ciferrii. Compared to bacterial infections, fungal infections pose challenges due to limited drug options, significant side effects, and prolonged treatment durations. Hence, prompt pathogen diagnosis and drug sensitivity testing are crucial for effective clinical treatment. In essence, this scientific case report underscores the uncommon occurrence of catheter-related peritonitis attributed to Stephanoascus ciferrii in a peritoneal dialysis patient with renal failure, emphasizing the distinctive management challenges and underscoring the critical significance of prompt diagnosis and suitable intervention in such instances.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 2","pages":"186-189"},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141635896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aerobic vaginitis: antibiotic resistance trend and future actions of antimicrobial diagnostic stewardship. 需氧性阴道炎：抗生素耐药性趋势和抗菌诊断管理的未来行动。

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-07-01

Francesco Foglia, Maria Teresa Della Rocca, Fortunato Montella, Maria Vasco, Annalisa Chianese, Carla Zannella, Anna De Filippis, Emiliana Finamore, Massimiliano Galdiero

{"title":"Aerobic vaginitis: antibiotic resistance trend and future actions of antimicrobial diagnostic stewardship.","authors":"Francesco Foglia, Maria Teresa Della Rocca, Fortunato Montella, Maria Vasco, Annalisa Chianese, Carla Zannella, Anna De Filippis, Emiliana Finamore, Massimiliano Galdiero","doi":"","DOIUrl":"","url":null,"abstract":"The study objective is to examine epidemiological and microbiological aspects of aerobic vaginitis in female patients admitted to University Hospital of Campania \"L. Vanvitelli\" over five years. The most represented strains were E. coli (n = 153), Citrobacter spp. increasing from 2020, E. faecalis (n = 149), S. haemolitycus (n = 61), and Candida albicans (n = 87). The susceptibility patterns of a selection of gram-negative and gram-positive representative bacterial isolates were examined. Carbapenems, aminoglycosides, and fosfomycin were most effective against gram-negative bacteria, whereas vancomycin, daptomycin, and linezolid exhibited greater efficacy against gram-positive bacteria. None of the E. coli and Citrobacter spp. isolates produced extended-spectrum beta-lactamases, and the S. haemolyticus strains were methicillin-resistant. In gram-positive isolates, gentamicin susceptibility increased in 2020 and 2021 compared to clindamycin; erythromycin showed high resistance rates in 2020. Our findings indicate that integrating proper microbiological cultures into clinical practice could improve the management of aerobic vaginitis. Moreover, they highlight the necessity of establishing a nationwide surveillance guideline to mitigate antimicrobial resistance. Improvement actions in antimicrobial diagnostic stewardship must be considered when seeking the appropriate diagnosis and treatment for aerobic vaginitis.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 2","pages":"164-171"},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141635890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Whole Blood and Plasma for Monitoring Cytomegalovirus and Epstein-Barr Virus. 比较全血和血浆在监测巨细胞病毒和 Epstein-Barr 病毒方面的作用。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-05-01

Yuto Fukuda, Yuka Torii, Ken-Ichi Iwata, Kazunori Haruta, Makoto Yamaguchi, Takako Suzuki, Atsushi Narita, Hideki Muramatsu, Yasuhiro Ogura, Yoshiyuki Takahashi, Yoshinori Ito, Jun-Ichi Kawada

{"title":"Comparison of Whole Blood and Plasma for Monitoring Cytomegalovirus and Epstein-Barr Virus.","authors":"Yuto Fukuda, Yuka Torii, Ken-Ichi Iwata, Kazunori Haruta, Makoto Yamaguchi, Takako Suzuki, Atsushi Narita, Hideki Muramatsu, Yasuhiro Ogura, Yoshiyuki Takahashi, Yoshinori Ito, Jun-Ichi Kawada","doi":"","DOIUrl":"","url":null,"abstract":"Monitoring Epstein-Barr virus (EBV) and cytomegalovirus (CMV) infection after transplantation is recommended to enable preemptive therapy. However, the most suitable sample type remains unclear. Patients who underwent hematopoietic stem cell or liver transplantation were included in this study. Viral loads in sequential whole-blood and plasma samples were retrospectively analyzed. EBV DNA was detected more frequently in whole blood (55%) than in plasma (18%). The detection rate of CMV DNA was similar between the two sample types. The correlation of viral loads between the two sample types were 0.515 and 0.688 for EBV and CMV, respectively. Among paired samples in which EBV DNA was detected in whole blood, the plasma EBV detection rate was significantly higher in patients who underwent hematopoietic stem cell transplantation than in those who underwent liver transplantation. The viral DNA load in whole blood and plasma showed similar trends. The EBV detection rate was higher in whole blood, and a high correlation was observed between CMV DNA loads and whole blood and plasma. These results indicate that whole blood is more sensitive for monitoring both EBV and CMV, whereas plasma is a potential alternative sample for monitoring CMV.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"52-59"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRESTIGIO RING "a 59-year-old man with multidrug resistant HIV-1 infection failing a regimen including dolutegravir, rilpivirine, atazanavir/cobicistat: successful treatment tailoring based on genotypic and phenotypic resistance tests". PRESTIGIO RING："一名 59 岁的 HIV-1 多药耐药感染者，在使用多鲁曲韦、利匹韦林、阿扎那韦/可比司他治疗方案后失败：根据基因型和表型耐药性测试成功定制了治疗方案"。

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-05-01

Francesca Panza, Francesco Saladini, Niccolò Bartolini, Federica Gianmarino, Francesca Montagnani, Vincenzo Spagnuolo, Mario Tumbarello, Maria Mercedes Santoro, Antonella Castagna, Maurizio Zazzi, Massimiliano Fabbiani

{"title":"PRESTIGIO RING \"a 59-year-old man with multidrug resistant HIV-1 infection failing a regimen including dolutegravir, rilpivirine, atazanavir/cobicistat: successful treatment tailoring based on genotypic and phenotypic resistance tests\".","authors":"Francesca Panza, Francesco Saladini, Niccolò Bartolini, Federica Gianmarino, Francesca Montagnani, Vincenzo Spagnuolo, Mario Tumbarello, Maria Mercedes Santoro, Antonella Castagna, Maurizio Zazzi, Massimiliano Fabbiani","doi":"","DOIUrl":"","url":null,"abstract":"Management of virological failure in heavily treatment-experienced people with multidrug-resistant (MDR) HIV infection is a serious clinical challenge. New drugs with novel mechanisms of action have recently been approved, and their use has improved the outcome of subjects with limited treatment options (LTO). In this setting, the choice of antiretroviral therapy (ART) should be tailored based on the pattern of resistance, treatment history and patients' individual characteristics. While genotypic resistance testing is the reference method for analysing residual drug susceptibility, phenotypic resistance testing can provide additional support when facing LTO. Herein, we present the case of a patient with MDR HIV-1 infection on virological failure enrolled in the PRESTIGIO Registry. The salvage ART regimen, which included drugs with novel mechanisms of action (MoA), was tailored to the patient's clinical characteristics and on the resistance pattern explored with genotypic and phenotypic investigation, allowing the achievement of viro-immunological success. The use of recently approved drugs with novel MoA, combined with an optimized background regimen, may also achieve virological suppression in people with LTO.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"116-122"},"PeriodicalIF":1.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140855492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of an automated rapid phenotypic antimicrobial susceptibility testing (ASTar, Q-linea AB) applied directly on blood cultures bottles positive for Gram-negative pathogens. 对直接用于革兰氏阴性病原体阳性血培养瓶的自动快速表型抗菌药物敏感性测试（ASTar，Q-linea AB）进行评估。

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-05-01

Isabella Banchini, Eva Caterina Borgatti, Claudio Foschi, Tiziana Lazzarotto, Simone Ambretti

引用次数: 0

Coinfection with respiratory syncytial virus and rhinovirus increases IFN-λ1 and CXCL10 expression in human primary bronchial epithelial cells. 呼吸道合胞病毒和鼻病毒双重感染会增加人原发性支气管上皮细胞中 IFN-λ1 和 CXCL10 的表达。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-05-01

Nipaporn Sankuntaw, Nuntaya Punyadee, Wasun Chantratita, Viraphong Lulitanond

{"title":"Coinfection with respiratory syncytial virus and rhinovirus increases IFN-λ1 and CXCL10 expression in human primary bronchial epithelial cells.","authors":"Nipaporn Sankuntaw, Nuntaya Punyadee, Wasun Chantratita, Viraphong Lulitanond","doi":"","DOIUrl":"","url":null,"abstract":"Acute respiratory tract infection (ARTI) is common in all age groups, especially in children and the elderly. About 85% of children who present with bronchiolitis are infected with respiratory syncytial virus (RSV); however, nearly one-third are coinfected with another respiratory virus, such as human rhinovirus (HRV). Therefore, it is necessary to explore the immune response to coinfection to better understand the molecular and cellular pathways involving virus-virus interactions that might be modulated by innate immunity and additional host cell response mechanisms. This study aims to investigate the host innate immune response against RSV-HRV coinfection compared with monoinfection. Human primary bronchial/tracheal epithelial cells (HPECs) were infected with RSV, HRV, or coinfected with both viruses, and the infected cells were collected at 48 and 72 hours. Gene expression profiles of IL-6, CCL5, TNF-α, IFN-β, IFN-λ1, CXCL10, IL-10, IL-13, IRF3, and IRF7 were investigated using real-time quantitative PCR, which revealed that RSV-infected cells exhibited increased expression of IL-10, whereas HRV infection increased the expression of CXCL10, IL-10, and CCL5. IFN-λ1 and CXCL10 expression was significantly different between the coinfection and monoinfection groups. In conclusion, our study revealed that two important cytokines, IFN-λ1 and CXCL10, exhibited increased expression during coinfection.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"60-67"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140862615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiology and molecular characterization of respiratory viruses at the end of COVID-19 pandemic in Lombardy, Northern Italy. 意大利北部伦巴第 COVID-19 大流行结束时呼吸道病毒的流行病学和分子特征。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-05-01

Leonardo Sclavi, Marta Bertelli, Serena Messali, Arnaldo Caruso, Francesca Caccuri

{"title":"Epidemiology and molecular characterization of respiratory viruses at the end of COVID-19 pandemic in Lombardy, Northern Italy.","authors":"Leonardo Sclavi, Marta Bertelli, Serena Messali, Arnaldo Caruso, Francesca Caccuri","doi":"","DOIUrl":"","url":null,"abstract":"The COVID-19 pandemic forced the adoption of non-pharmaceutical interventions (NPIs) which influenced the circulation of other respiratory pathogens, such as Influenza virus (FLU), Parainfluenza virus (PIV), Respiratory Syncytial virus (RSV), Rhinovirus (RV), Enterovirus (EV), Adenovirus (AdV), Human Metapneumovirus (hMPV), and Human Coronavirus (CoV). The aim of the current study was to investigate how, with the end of the pandemic, the withdrawal of the NPIs impacted on the circulation and distribution of common respiratory viruses. The analyzed samples were collected from June 2021 to March 2023 (post-pandemic period) and compared to ones from the pandemic period. Nucleic acid detection of all respiratory viruses was performed by multiplex real time Polymerase Chain Reaction (PCR) and sequencing was conducted by Next Generation Sequencing (NGS) technique. Our analysis shows that the NPIs adopted against SARS-CoV-2 were also effective in controlling the spread of other respiratory viruses. Moreover, we documented how RV/EVs were the most commonly identified species, with the more abundant strains represented by Coxsackievirus (CV)-A/B and RV-A/C. RV/EVs were also detected in some co-infection cases; in particular, the majority of co-infections concerned CV-B/RV-A, CV-B/ECHO. Given the pandemic potential of respiratory viruses, accurate molecular screening is essential for a proper surveillance and prevention strategy.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"80-87"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lamivudine plus dolutegravir as a switch strategy in children: three case reports. 拉米夫定加多鲁曲韦作为儿童的转换策略：三份病例报告。

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-05-01

Laura Labate, Claudia Bartalucci, Lucia Taramasso, Giorgia Brucci, Antonio Vena, Matteo Bassetti, Antonio Di Biagio

{"title":"Lamivudine plus dolutegravir as a switch strategy in children: three case reports.","authors":"Laura Labate, Claudia Bartalucci, Lucia Taramasso, Giorgia Brucci, Antonio Vena, Matteo Bassetti, Antonio Di Biagio","doi":"","DOIUrl":"","url":null,"abstract":"Lamivudine (3TC)/dolutegravir (DTG) single tablet regimen (STR) has shown long-term efficacy and tolerability in people living with HIV (PLWH). Dolutegravir has been approved for use in children, while data on the efficacy of 3TC plus DTG in maintaining virological suppression in this population are still under evaluation. In this case series, we describe three children with perinatally acquired HIV who maintained virological suppression after switching antiretroviral therapy to DTG/3TC. We present three case reports of three children enrolled in the Italian Register for HIV Infection in Children: a 9-year-old boy, a 10-year-old girl, and a 2-year-old girl with perinatally acquired HIV who immediately started antiretroviral therapy with a three-drug regimen upon diagnosis, which occurred at delivery, after 6 months of life, and after 2 years of life, respectively. They achieved and maintain virological suppression after 1, 6, and 7 months of therapy, respectively; then a switch strategy was performed with a two-drug regimen with DTG/3TC STR at the age of 7 years for the first child and at the age of 9 years for the second, while the third was switched to a DTG plus 3TC not STR, owing to weight requirements, at the age of 2 years and 10 months. All children maintained virological suppression at last follow-up visit (January 2024), showing an excellent growth curve and maintaining good adherence and tolerability to DTG plus 3TC. A two-drug regimen with DTG/3TC demonstrated efficacy in maintaining virological suppression in a switch strategy in these children, with important advantages such as better tolerability and comfort of taking a single tablet once daily.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"111-115"},"PeriodicalIF":1.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140863304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Risk Human Papilloma Virus Genotype Distribution and Correlation with Cervical Cytomorphological Data in Turkish and Immigrant Women in Mersin Province. 梅尔辛省土耳其妇女和移民妇女的高危人类乳头瘤病毒基因型分布及其与宫颈细胞形态学数据的相关性

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-05-01

Murat Yaman, Hacer Ece Arslan Özcan, Ayfer Bakir

{"title":"High-Risk Human Papilloma Virus Genotype Distribution and Correlation with Cervical Cytomorphological Data in Turkish and Immigrant Women in Mersin Province.","authors":"Murat Yaman, Hacer Ece Arslan Özcan, Ayfer Bakir","doi":"","DOIUrl":"","url":null,"abstract":"Human papilloma virus (HPV) is the most common sexually transmitted viral agent in the world and the most common cause of cervical cancer. HPV prevalence and genotype distribution vary by region and demographic data. In a province in the south of Turkey that constantly receives immigration, we aimed to determine the prevalence of high-risk HPV (HR-HPV) genotypes, evaluate the compatibility between cervical Pap smear cytology results patients and HR-HPVs, and make an up-to-date contribution to the elucidation of epidemiological data. In this single-centre study, a total of 12,641 women aged 18 and over were evaluated retrospectively from January 2019 to July 2022. HPV detection and genotyping were analysed by the PCR method. Bethesda scoring was used for Pap smear cytological evaluation. The overall prevalence of HR-HPV was 12.6% (12.7% in Turkish women, 11.2% in foreign women). Among the typed HPVs that were detected, HPV-16 (31%) was found first, followed by HPV-18 (8%). The prevalence of HR-HPV was higher in women with abnormal cytology (977/1762, 55.4%) than in women with normal cytology (620/10879, 5.7%) (p<0.001). Turkey doesn't yet have a national HPV immunisation program. We think that determining the specific regional frequency of other HR-HPVs separately will be useful in the follow-up of the natural course of the type-specific infection and in vaccine studies in the future.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"88-97"},"PeriodicalIF":1.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140866074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long COVID: lights and shadows on the clinical characterization of this emerging pathology. 长COVID：这一新兴病理临床特征的光与影。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-05-01

Viola Cogliandro, Paolo Bonfanti

引用次数: 0