Progress in Biophysics & Molecular Biology最新文献_第3页

Exercise as a therapeutic strategy against mitochondrial dysfunction in ischemic stroke: Molecular mechanisms and perspectives for personalized treatment 运动作为对抗缺血性脑卒中线粒体功能障碍的治疗策略：分子机制和个性化治疗的观点。

IF 4.5 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-10-15 DOI: 10.1016/j.pbiomolbio.2025.10.002

Xinzheng Wang , Huifen Zhou

{"title":"Exercise as a therapeutic strategy against mitochondrial dysfunction in ischemic stroke: Molecular mechanisms and perspectives for personalized treatment","authors":"Xinzheng Wang , Huifen Zhou","doi":"10.1016/j.pbiomolbio.2025.10.002","DOIUrl":"10.1016/j.pbiomolbio.2025.10.002","url":null,"abstract":"<div><div>Ischemic stroke (IS) is a leading cause of disability and mortality worldwide, with mitochondrial dysfunction being a fundamental pathological mechanism. This dysfunction involves a dynamic imbalance, diminished biosynthesis, oxidative stress, and dysregulated autophagy. Exercise, a promising non-pharmacological intervention, can ameliorate this dysfunction, but its precise molecular mechanisms remain to be fully elucidated. This review synthesizes evidence demonstrating that exercise enhances mitochondrial morphology and function through various pathways, including the promotion of mitochondrial biogenesis, the regulation of mitochondrial ROS, and the modulation of mitochondrial dynamics and mitophagy, thereby mitigating functional impairments associated with IS. Critically, the beneficial effects of exercise are dose dependent, highlighting the necessity for personalized exercise prescriptions on the basis of individual patient profiles. Elucidating these mechanisms provides a crucial theoretical foundation for developing exercise-based strategies for the prevention and treatment of IS.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"198 ","pages":"Pages 55-60"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145314128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Delineation and functions of HCN channels in neurons 神经元中HCN通道的描绘和功能。

IF 4.5 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-09-13 DOI: 10.1016/j.pbiomolbio.2025.09.002

Sodikdjon A. Kodirov

{"title":"Delineation and functions of HCN channels in neurons","authors":"Sodikdjon A. Kodirov","doi":"10.1016/j.pbiomolbio.2025.09.002","DOIUrl":"10.1016/j.pbiomolbio.2025.09.002","url":null,"abstract":"<div><div>The observation of non-linear current-voltage relationships of membrane potential responses in neurons led to the discovery of hyperpolarization-induced membrane conductance. The main underlying hallmark of this conductance was the presence of sag – spontaneous depolarization of membrane potential during constant hyperpolarization in current-clamp mode. Gradually, the presence of underling hyperpolarization-activated cyclic nucleotide-gated non-selective cation channels (HCN, <em>I</em><sub>h</sub>, or <em>I</em><sub>f</sub>, where <em>f</em> is for funny) was established. The earliest delineation of HCN-mediated sag in the hippocampus was documented by Purpura, Prelevic, and Santini with a short communication in 1968. The study was performed using classical electrophysiology by impaling the perikaryon of hippocampal neurons, but the outcomes are as insightful as with the patch-clamp technique, and the quality of traces exceeds some of the recent recordings. However, the latter authors were not convinced that the sag phenomenon could play a role under physiological conditions. It is logical since a depolarization and linked excitability are frequently observed in <em>vivo</em>, but not a hyperpolarization. At least a sudden, sharp hyperpolarization does not occur in the CNS. Besides, in order to activate the HCN channels, the amplitude and duration of hyperpolarization should be significant and sufficient. Nonetheless, those with skepticism conveyed pioneer observations were pivotal, since the presence of sag – activation of HCN channels – may also play a role in autism and epilepsy.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"198 ","pages":"Pages 21-31"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145071257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell aging - a relevant factor in live cell microscopy (mini-review) 细胞老化——活细胞显微术中的一个相关因素。

IF 4.5 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-10-31 DOI: 10.1016/j.pbiomolbio.2025.10.003

Tina Karimian , Christoph Cremer , Julian Weghuber , Herbert Schneckenburger

{"title":"Cell aging - a relevant factor in live cell microscopy (mini-review)","authors":"Tina Karimian , Christoph Cremer , Julian Weghuber , Herbert Schneckenburger","doi":"10.1016/j.pbiomolbio.2025.10.003","DOIUrl":"10.1016/j.pbiomolbio.2025.10.003","url":null,"abstract":"<div><div>Live-cell microscopy is gaining importance for studying cellular behavior in response to environmental cues. However, cell aging can result in modifications of various cellular structures and functions, affecting or distorting microscopy-based readouts. These changes include gene expression, nuclear architecture, energy metabolism, or changes in the mechanical properties of cell membranes and microtubules. In this mini-review, we briefly discuss how cell aging affects critical subcellular compartments and alters live-cell imaging outcomes. In contrast to many papers available on cell aging, here we are focusing on the influence of cell aging on the performance and outcome of advanced microscopy techniques such as super-resolution imaging, fluorescence lifetime imaging (FLIM), variable-angle total internal reflection fluorescence microscopy (VA-TIRFM), as well as micromanipulation techniques such as laser-assisted optoporation. Our findings highlight the importance of considering cell passage number and senescence markers in experimental design and data interpretation.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"198 ","pages":"Pages 61-70"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145427166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the parallels between nanoparticles and viruses with emphasis on environmental roles and remediation 探索纳米颗粒和病毒之间的相似之处，重点是环境作用和补救。

IF 4.5 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-09-29 DOI: 10.1016/j.pbiomolbio.2025.09.003

Mahima Kaushik , Madhu Pruthi , Arpana Sharma , Radhey Shyam Sharma , Vandana Mishra , Swagata Karmakar , Niloy Sarkar

{"title":"Exploring the parallels between nanoparticles and viruses with emphasis on environmental roles and remediation","authors":"Mahima Kaushik , Madhu Pruthi , Arpana Sharma , Radhey Shyam Sharma , Vandana Mishra , Swagata Karmakar , Niloy Sarkar","doi":"10.1016/j.pbiomolbio.2025.09.003","DOIUrl":"10.1016/j.pbiomolbio.2025.09.003","url":null,"abstract":"<div><div>Nanoparticles and viruses share several similarities and link the fields of physical and biological sciences by covering and overlapping both realms. In this review we have discussed their commonalities based on classification of constituent material, mode of replication and origin, size, as well as function and application in cargo delivery. We have discussed the modulatory and characteristics of each subunit and how it contributes to cellular uptake and replication. We have also gone into their environmental roles in nutrient mobilization and cycling, being subject to and themselves exerting evolutionary pressures as well as their final environmental fates of immobilization and disintegration. Finally, we have explored their potential use in environmental remediation and energy generation. The novelty of this work is in signifying that both nanoparticles and viruses fall into the realm of macro-biomolecular or nano assemblies and are in many ways similar in origin, characteristics and can be often used synergistically to solve contemporary problems.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"198 ","pages":"Pages 39-54"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cationic calcium channels activated by cyclic nucleotides in plants: A systematic review using the PRISMA method 植物中环核苷酸激活的阳离子钙通道：基于PRISMA方法的系统综述。

IF 4.5 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-09-06 DOI: 10.1016/j.pbiomolbio.2025.09.001

Camilo Tayac , J. Torres-Osorio , José Mauricio Rodas-Rodríguez

{"title":"Cationic calcium channels activated by cyclic nucleotides in plants: A systematic review using the PRISMA method","authors":"Camilo Tayac , J. Torres-Osorio , José Mauricio Rodas-Rodríguez","doi":"10.1016/j.pbiomolbio.2025.09.001","DOIUrl":"10.1016/j.pbiomolbio.2025.09.001","url":null,"abstract":"<div><div>The primary objective of this review is to analyze primary research published over the past six years concerning cyclic nucleotide-gated calcium channels (CNGC) in plants. The aim is to structure this information to identify and organize existing knowledge regarding their tertiary and quaternary structures, as well as the activation mechanisms of CNGC. Studies on plant CNGC published between January 2018 and May 2025 were included, while research focused on animals, bacteria, or ions other than calcium was excluded. Articles were retrieved from Scopus, Web of Science, and PubMed databases through searches conducted between June 2024 and May 2025, as well as from additional personal sources. A total of 111 articles met the inclusion criteria. These were categorized into seven groups: phylogenetic analysis and classification of plant CNGC monomers, gene expression regulation, structural composition of monomers and tetramers, channel activation, selectivity mechanisms, cellular localization, and the plant species and structural types in which they are found. The findings revealed that CNGC can be activated by individual mechanisms or by the interplay of multiple pathways. However, uncertainties remain regarding certain activation processes. A lack of experimental studies specifically aimed at elucidating their crystallographic structure was evident, which limits a comprehensive understanding of these channels and represents one of the main constraints of the available evidence. This limitation highlights the need for further research to fully elucidate both the activation mechanisms and structural characteristics of CNGC. Despite these constraints, the findings indicate that CNGC play a pivotal role in plant physiology.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"198 ","pages":"Pages 8-20"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The DOPA scaffold: Tracing catechol chemistry from prebiotic earth to cognitive agency 多巴支架：从益生元地球到认知代理追踪儿茶酚化学。

IF 4.5 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-11-01 DOI: 10.1016/j.pbiomolbio.2025.10.004

Amal Alachkar

{"title":"The DOPA scaffold: Tracing catechol chemistry from prebiotic earth to cognitive agency","authors":"Amal Alachkar","doi":"10.1016/j.pbiomolbio.2025.10.004","DOIUrl":"10.1016/j.pbiomolbio.2025.10.004","url":null,"abstract":"<div><div>L-3,4-dihydroxyphenylalanine (DOPA) is primarily defined by its role as the precursor to catecholamine neurotransmitters, a view substantiated by its status as the gold-standard treatment for Parkinson's disease. This framing obscures its profound evolutionary significance. This review reframes DOPA as a fundamental physicochemical scaffold whose core aromatic catechol-based properties: electron transfer, metal chelation, self-assembly, and polymerization into semiconducting biopolymers have been repeatedly repurposed throughout three billion years, from prebiotic Earth to the human brain.</div><div>From its plausible origins in prebiotic catalysis, these properties were leveraged by life to solve recurring challenges in survival and protection (e.g., microbial siderophores, protective melanins), settlement and colonization (e.g., marine bioadhesives), and competitive/cooperative communication (e.g., allelochemicals, social signaling). This evolutionary history of DOPA reveals a consistent pattern, a conserved 'Stress-Motion-Motivation-Action’ arc that links environmental challenges to the emergence of goal-directed behavior. I propose that DOPA's same ancient chemical logic was not discarded but repurposed in the mammalian brain. Beyond serving as a dopamine precursor, DOPA's non-canonical functions: adhesion, chelation, and polymerization into the semiconducting biopolymer neuromelanin, form a multiscale biophysical scaffold. This scaffold provides the structural and electrochemical stability necessary for the neural circuits and for the canonical dopamine signaling to execute not only motivation and movement but also the agency-related complex computations. This model thus bridges molecular biophysics with higher-order cognition, reframing DOPA as a fundamental material substrate for cognitive agency.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"198 ","pages":"Pages 71-91"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145439941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A guide to transport-of-intensity equation (TIE) imaging for biologists 生物学家的强度传输方程（TIE）成像指南

IF 4.5 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-08-18 DOI: 10.1016/j.pbiomolbio.2025.08.001

N.C. Ruppel, M.A. Model

{"title":"A guide to transport-of-intensity equation (TIE) imaging for biologists","authors":"N.C. Ruppel, M.A. Model","doi":"10.1016/j.pbiomolbio.2025.08.001","DOIUrl":"10.1016/j.pbiomolbio.2025.08.001","url":null,"abstract":"<div><div>The measurement of dry cell mass (often referred to as “protein”) under the microscope can be accomplished using a quantitative phase imaging technique known as Transport of Intensity Equation (TIE) microscopy. This method requires no specialized equipment, relying instead on two slightly defocused brightfield images acquired with a standard optical microscope. The images are processed by the TIE equation to convert the gradient of intensity into phase shifts and ultimately a distribution of protein mass. Beyond its simplicity, a major advantage of TIE over most other quantitative phase methods is its compatibility with fluorescence and with cell volume measurements. When paired with volume data, TIE enables the conversion of protein mass into the biologically significant parameters of protein concentration and intracellular water content.</div><div>This review emphasizes practical implementation, including calibration, focal plane selection, reproducibility, image size effects, strategies for artifact reduction, as well as the biological relevance of the recovered phase. We also describe an easy-to-use Fiji plugin for solving the TIE equation, eliminating the need for advanced computational tools.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"198 ","pages":"Pages 1-7"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taurine up-regulated 1: A dual regulator in immune cell-mediated pathogenesis of human diseases 牛磺酸上调1：免疫细胞介导的人类疾病发病机制中的双重调节因子。

IF 3.2 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-09-01 Epub Date: 2025-07-15 DOI: 10.1016/j.pbiomolbio.2025.07.002

Xin Lan , Shuwen Zhang , Lu Yang

{"title":"Taurine up-regulated 1: A dual regulator in immune cell-mediated pathogenesis of human diseases","authors":"Xin Lan , Shuwen Zhang , Lu Yang","doi":"10.1016/j.pbiomolbio.2025.07.002","DOIUrl":"10.1016/j.pbiomolbio.2025.07.002","url":null,"abstract":"<div><div>Taurine up-regulated 1 (TUG1) is a long non-coding RNA (lncRNA) that plays a significant role in the pathogenesis of both cancer and non-cancer diseases. Recent studies have revealed its involvement in regulating the development of various diseases by modulating the activity of the body's immune cells. In non-cancer diseases, TUG1 primarily influences disease progression through the competing endogenous RNA (ceRNA) network, promoting the expression of pro-inflammatory cytokines via pathways such as the NF-κB inflammatory signaling pathway. Most research indicates that TUG1 exerts a positive regulatory effect on immune cells, including Th2 cells, M1 macrophages, and microglia. In cancer, TUG1 regulates disease progression predominantly through the ceRNA network and by modulating the activity of specific transcription factors. It fosters tumor development by promoting the establishment of immune tolerance within the tumor microenvironment. This immune tolerance is associated with TUG1's regulation of immune checkpoint molecules, which enhances the infiltration of pro-tumor immune cells (e.g., regulatory T cells, M2 macrophages, neutrophils, and dendritic cells) while suppressing the infiltration of anti-tumor immune cells, including CD8<sup>+</sup> T cells, NK cells, and M1 macrophages. In this study, we systematically evaluate the impact of abnormal TUG1 expression across various diseases, focusing on its mechanisms of action in regulating immune cell infiltration and disease progression in both cancer and non-cancer contexts. We also discuss potential targets for future research related to TUG1's role in these pathogenic processes.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"197 ","pages":"Pages 84-96"},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144661019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid droplet - organelle crosstalk and its implication in cancer 脂滴-细胞器串扰及其在癌症中的意义。

IF 3.2 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-09-01 Epub Date: 2025-05-15 DOI: 10.1016/j.pbiomolbio.2025.05.002

Jing Quan , Chunhong Zhang , Xue Chen , Xinfei Cai , Xiangjian Luo

引用次数: 0

Advances and challenges in the treatment of osteosarcoma 骨肉瘤治疗的进展与挑战。

IF 3.2 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-09-01 Epub Date: 2025-07-04 DOI: 10.1016/j.pbiomolbio.2025.07.001

Xingkai Wang , Kunpeng Zhu , Jianping Hu , Chunlin Zhang

{"title":"Advances and challenges in the treatment of osteosarcoma","authors":"Xingkai Wang , Kunpeng Zhu , Jianping Hu , Chunlin Zhang","doi":"10.1016/j.pbiomolbio.2025.07.001","DOIUrl":"10.1016/j.pbiomolbio.2025.07.001","url":null,"abstract":"<div><div>Osteosarcoma represents the greatest percentage of malignant tumors in the bone and is distinguished by its high incidence of aggressiveness, metastasis, and recurrence after therapy. At present, the treatment strategies for osteosarcoma typically encompass surgical resection, chemotherapy, targeted therapy, and radiotherapy. Even though there are now more extensive treatment options for osteosarcoma, the prognosis is still dismal, particularly for those who have metastatic osteosarcoma. Developing efficient treatment approaches therefore becomes crucial to turning things around. With the ongoing advancements in science and technology, together with the integration of research in diverse domains, more creative approaches to treating osteosarcoma have been explored in recent years. This article reviews the cutting-edge advances in osteosarcoma immunotherapy, biomedical materials, and gut microbiota in the field of osteosarcoma treatment. Ultimately, our goal is to increase the overall survival rate for patients with osteosarcoma by providing robust theoretical support for the development of innovative medications through an in-depth analysis and summary of the most recent advancements and obstacles facing osteosarcoma treatment.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"197 ","pages":"Pages 60-74"},"PeriodicalIF":3.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0