{"title":"[Detection of Gamma-Ray Irradiation History of Dried Plant-Based Foods via the Analysis of 5,6-Dihydrothymidine].","authors":"Naoki Fukui, Takuya Fujiwara, Masakazu Furuta, Satoshi Takatori","doi":"10.3358/shokueishi.64.206","DOIUrl":"10.3358/shokueishi.64.206","url":null,"abstract":"<p><p>Irradiation is widely used worldwide to sterilize and kill insects in food, and prevent the germination of agricultural products. However, in Japan, food irradiation is prohibited except to prevent potato sprouting. Herein, 5,6-dihydrothymidine (DHdThd) residue-a damaged nucleoside generated from the thymidine (dThd) residue in DNA contained in food upon irradiation-was used as a detection indicator. Eight dried plant-based food samples were gamma ray-irradiated in the range from 3.2 to 8.3 kGy. Subsequently, DNA was extracted from the irradiated sample and digested into nucleosides by the three enzymes, and the test solution was analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). Evidently, in all samples, the concentration ratio of DHdThd to dThd in the test solution (DHdThd/dThd) was dependent on the irradiation dose; moreover, during storage under frozen conditions for at least 890 d post-irradiation, this concentration ratio was equal to that immediately after irradiation. The irradiation histories of the eight types of dried plant-based food samples were correctly detected.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"64 6","pages":"206-213"},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139089374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Quantitative Survey of Campylobacter on Chicken Livers in Japan].","authors":"Yoshimasa Sasaki, Kenzo Yonemitsu, Yoshika Momose, Masashi Uema","doi":"10.3358/shokueishi.64.214","DOIUrl":"10.3358/shokueishi.64.214","url":null,"abstract":"<p><p>Chicken liver is a potential source of campylobacteriosis in humans. Therefore, we determined the number of Campylobacter in chicken liver. In total, 33 vacuum-packed liver products were obtained from retail stores, and found that 27 of the 33 products (81.8%) were contaminated with Campylobacter. Moreover, Campylobacter was isolated from 138 of 149 livers (92.6%) collected from the 27 Campylobacter-positive products. The mean Campylobacter count was 2.3 log<sub>10</sub> CFU/g, while Campylobacter count in 22 of the 138 contaminated livers (15.9%) was >3.0 log<sub>10</sub> CFU/g. Furthermore, gastrointestinal tract, liver, and bile samples were collected from 35 broilers at chicken processing plants. We isolated Campylobacter from the gastrointestinal tract of 27 broilers (77.1%). Of these 27 broilers, liver of 24 broilers (88.9%) was Campylobacter-positive, with a mean Campylobacter count of 2.8 log<sub>10</sub> CFU/g. Of these 24 broilers, bile of 13 broilers (54.2%) was contaminated with Campylobacter (mean Campylobacter count, 3.5 log<sub>10</sub> CFU/mL). Among them, bile of 2 broilers had a Campylobacter count of >8.3 log<sub>10</sub> CFU/mL. Collectively, these results indicate that livers derived from broilers colonized with Campylobacter are contaminated with Campylobacter at the time of evisceration. Therefore, to prevent foodborne campylobacteriosis in humans, chicken livers should be thoroughly heated before consumption.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"64 6","pages":"214-217"},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139089377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ryohei Tatsuno, Ryo Yamasaki, Kai Mizokami, Genki Hayashi, Tsubasa Fukuda, Manabu Furushita, Hiroshi Takahashi, Takayuki Sonoyama, Shigeo Hori
{"title":"[Tetrodotoxin Content of the Small Gastropod Nassarius sufflatus].","authors":"Ryohei Tatsuno, Ryo Yamasaki, Kai Mizokami, Genki Hayashi, Tsubasa Fukuda, Manabu Furushita, Hiroshi Takahashi, Takayuki Sonoyama, Shigeo Hori","doi":"10.3358/shokueishi.64.236","DOIUrl":"10.3358/shokueishi.64.236","url":null,"abstract":"<p><p>Severe tetrodotoxin (TTX) poisoning due to small gastropods has been documented in Japan. In this study, we investigated the TTX content of the muscles and viscera of Nassarius sufflatus collected off the coast of Futaoi Island, Shimonoseki, Yamaguchi Prefecture, Japan, to prevent the occurrence of TTX poisoning caused by this small gastropod. Live specimens were obtained, and their muscles and viscera were collected. Test solutions were prepared from tissues of specimens and analyzed for TTX by HPLC-fluorescence detection. TTX was detected in both tissues at concentrations ranging from <0.1 to 18.2 μg/g for muscle and <0.1 to 130.7 μg/g for viscera. These results suggested that N. sufflatus accumulates TTX not only in its viscera but also in its muscles, and that precautions should be taken to prevent food poisoning due to this gastropod.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"64 6","pages":"236-239"},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139089380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Single-laboratory Validation Study and Surveillance Using an Improved Multiresidue Analytical Method for Veterinary Drugs in Livestock Products by LC-MS/MS].","authors":"Momoka Hayashi, Maki Kanda, Souichi Yoshikawa, Takayuki Nakajima, Hiroshi Hayashi, Yoko Matsushima, Yumi Ohba, Hiroshi Koike, Chieko Nagano, Kenji Otsuka, Takeo Sasamoto","doi":"10.3358/shokueishi.64.53","DOIUrl":"10.3358/shokueishi.64.53","url":null,"abstract":"<p><p>A method for the rapid analysis of multiclass residual veterinary drugs in poultry muscle, egg, and raw milk was validated in accordance with Japanese guidelines. Using LC-MS/MS, 20 veterinary drugs, including sulfonamides, coccidiostats, and macrolides were analyzed in one injection. Analytes were extracted from the samples with acetonitrile and then dehydrated and salted out using magnesium sulfate, trisodium citrate, and sodium chloride. This method was assessed by performing recovery tests of chicken muscle, duck muscle, egg, and raw milk spiked with 20 new target analytes at concentrations of 10 and 100 µg/kg. According to this method, 17 out of 20 target analytes satisfied the guideline criteria in chicken muscle and duck muscle, and all 20 target analytes met the criteria in egg and raw milk. The limit of quantification was less than MRLs for all analytes. Residues were detected in 4 out of 99 samples and analyzed using the validated method, finding that the levels of all residues were lower than the limits of quantification. These results suggest that continuous monitoring for a new trend of veterinary drugs is necessary.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"64 1","pages":"53-60"},"PeriodicalIF":0.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10823514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Ozaki, M. Mutsuga, Eri Kishi, T. Abe, Yutaka Abe, Keiko Ando, Kinuyo Ishihara, Atsuko Ushiya, Shinsaku Uchida, Ikue Ohsaka, H. Ohno, Takamitsu Kazama, Chika Kato, Hisashi Kobayashi, T. Sato, H. Shibata, H. Sekido, Hideo Takashima, Aoi Tanaka, H. Tonooka, Kotaro Hanazawa, Miku Yamaguchi, Satoshi Yamada, Mitsuhide Yoshikawa, Kazunari Watanabe, Kyoko Sato
{"title":"[Validation Study on Migration Test for Plastic Food Utensils, Containers, and Packaging].","authors":"A. Ozaki, M. Mutsuga, Eri Kishi, T. Abe, Yutaka Abe, Keiko Ando, Kinuyo Ishihara, Atsuko Ushiya, Shinsaku Uchida, Ikue Ohsaka, H. Ohno, Takamitsu Kazama, Chika Kato, Hisashi Kobayashi, T. Sato, H. Shibata, H. Sekido, Hideo Takashima, Aoi Tanaka, H. Tonooka, Kotaro Hanazawa, Miku Yamaguchi, Satoshi Yamada, Mitsuhide Yoshikawa, Kazunari Watanabe, Kyoko Sato","doi":"10.3358/shokueishi.63.51","DOIUrl":"https://doi.org/10.3358/shokueishi.63.51","url":null,"abstract":"Migration test of food utensils, containers, and packaging is an important test method for confirming the safety and their compliance to the standards. However, there is little report on inter-laboratory study which was performed to evaluate the entire migration test, including migration operations and quantification. An interlaboratory study was performed participating 22 laboratories using 8 types of model synthetic resin samples containing 10 substances with a wide range of Log Pow values to evaluate the accuracy of the entire migration test. As a result, most of HorRat (r) values met the target criteria (0.3<HorRat (r) ≦1.3), but many of HorRat (R) values exceed the target criteria (0.5<HorRat (R) ≦2.0). The results showed good accuracy within the laboratory. However, we found that there was a problem in accuracy between laboratories. The main reason was considered to be the difference in the preparation of migration solution between the laboratories, such as temperature and time control during the migration operation.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"23 1","pages":"51-61"},"PeriodicalIF":0.3,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75399341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yutaka Abe, T. Abe, H. Ohno, Kimiyasu Ohashi, A. Ozaki, Takamitsu Kazama, Yohei Kataoka, Kumi Suzuki, Shinichiro Nagai, Kotaro Hanazawa, Masato Hayakawa, Naoyuki Hirabayashi, Miku Yamaguchi, Kazunari Watanabe, M. Mutsuga, Kyoko Sato
{"title":"[Inter-laboratory Study on the Modified Method Assessment for the Determining Methanol Content in Kitchen Detergents].","authors":"Yutaka Abe, T. Abe, H. Ohno, Kimiyasu Ohashi, A. Ozaki, Takamitsu Kazama, Yohei Kataoka, Kumi Suzuki, Shinichiro Nagai, Kotaro Hanazawa, Masato Hayakawa, Naoyuki Hirabayashi, Miku Yamaguchi, Kazunari Watanabe, M. Mutsuga, Kyoko Sato","doi":"10.3358/shokueishi.63.97","DOIUrl":"https://doi.org/10.3358/shokueishi.63.97","url":null,"abstract":"We modified a method for determining methanol content in detergents used in kitchens. Furthermore, an inter-laboratory study was conducted in 10 laboratories to validate the modified method. In this study, two concentrations of samples were blindly coded. Each laboratory determined the methanol content in each sample according to a protocol. The determined values were statistically analyzed according to an international harmonized guideline. HorRat values were calculated based on the reproducibility relative standard deviation (RSDR) which was estimated from the interlaboratory study, and predicted RSDR calculated from the Horwitz/Thompson equation. The HorRat values of the two samples were 0.8 and 1.8, meeting the performance criteria of less than 2 set by the Codex Alimentarius for analytical method approval. These results confirm that this modified analytical method shows good performance as an analytical method for determining methanol content in kitchen detergents.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"48 1","pages":"97-103"},"PeriodicalIF":0.3,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83344334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Surveillance of Acaricides in Honey].","authors":"Yumi Ohba, Takayuki Nakajima, Maki Kanda, H. Hayashi, Chieko Nagano, Souichi Yoshikawa, Youko Matsushima, Hiroshi Koike, Momoka Hayashi, K. Otsuka, Takeo Sasamoto","doi":"10.3358/shokueishi.63.92","DOIUrl":"https://doi.org/10.3358/shokueishi.63.92","url":null,"abstract":"By using the LC-MS/MS method developed by us, we determined the residual amounts of acaricides in honey samples commercially available in Tokyo from April 2015 to March 2021. The results of analyzing 127 honey samples, amitraz was detected in 85 samples at the level of 1.1-34.1 μg/kg. Propargite was detected in 3 samples at 2.4-3.8 μg/kg. None of them was beyond the Japanese MRLs or uniform limits. In these survey for 6 years, amitraz was detected in high rate throughout the year. But, the present results imply that amitraz has been used properly in actual bee-keeping because of no violation of MRL and less fluctuation in the detected levels. On the other hand, propargite was detected at the levels over LOQ in domestic honey samples for the first time in 2020, which may suggest a new trend of acaricide use in apiculture in Japan.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"39 1","pages":"92-96"},"PeriodicalIF":0.3,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82441512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Ishida, H. Ouchi, T. Kan, H. Shinohara, Katsunori Nakajima, Yuka Nagaoka
{"title":"[Isolating Venomous Constituents of Paralepistopsis acromelalgaand Simultaneous Analysis of Its Compounds].","authors":"Y. Ishida, H. Ouchi, T. Kan, H. Shinohara, Katsunori Nakajima, Yuka Nagaoka","doi":"10.3358/shokueishi.63.79","DOIUrl":"https://doi.org/10.3358/shokueishi.63.79","url":null,"abstract":"An isolation method for Acromelic acids A, B and Clitidine, which are venomous constituents of Paralepistopsis acromelalga was developed. Highly purified products were obtained from the mushroom extract using silica gel, ODS, ion-exchange column chromatography and preparative TLC. Using those results, we optimized the LC-MS/MS conditions. Finally we developed a method for simultaneous analysis. In recovery tests, the average recovery was 80.8-112.4%, repeatability was 1.4-3.8RSD%. The limits of quantification of the respective compounds were estimated as 0.25 μg/g. Based on the results, this method can reveal causes of food poisoning by Paralepistopsis acromelalga.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"37 1","pages":"79-84"},"PeriodicalIF":0.3,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74643210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hiroyuki Hashimoto, Tatsuya M. Ikeda, Masato Yoshimitsu, Kyohei Kiyota
{"title":"[Secondary Contamination by Wheat Allergens across Cooking Bowls through the Use of Sponge Scourers].","authors":"Hiroyuki Hashimoto, Tatsuya M. Ikeda, Masato Yoshimitsu, Kyohei Kiyota","doi":"10.3358/shokueishi.63.70","DOIUrl":"https://doi.org/10.3358/shokueishi.63.70","url":null,"abstract":"After washing the cooking utensils used to prepare liquid batter, flour may remain in the scrubbing sponge. Secondary contamination of cooking devices with wheat allergens is a concern if such scrubbing sponges are used to clean other equipment. Therefore, in this study, bowl washing with detergent, using a scrubbing sponge was evaluated as a cleaning method following liquid batter cooking. Multiple trials revealed that wheat allergens were transferred from a bowl coated with 10 g of liquid batter to an unused bowl via sponge scrubbing with a positive rate of approximately 80%. Residual batter was observed in the scrubbing sponge after cleaning and rinsing procedures, with a residual rate of about 20%. Detailed observation of the residue revealed that proteins such as gluten were attached to the cell skeleton, and between the skeletons of the sponge, and starch granules were attached to the proteins. Even with sponge rinsing conditions added to the protocol, complete removal of wheat allergen was difficult. These studies indicate that particular scrubbing sponges for cleaning the residual wheat on cooking utensils should be dedicated exclusively in cooking facilities that prepare allergy-friendly-foods to avoid risk of allergen contamination.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"26 9 1","pages":"70-78"},"PeriodicalIF":0.3,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82550946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aiko Yutani, T. Nakatani, A. Ozaki, Yukihiko Yamaguchi, T. Yamano
{"title":"[Survey of Total Mercury Content in Fishery Products].","authors":"Aiko Yutani, T. Nakatani, A. Ozaki, Yukihiko Yamaguchi, T. Yamano","doi":"10.3358/shokueishi.63.85","DOIUrl":"https://doi.org/10.3358/shokueishi.63.85","url":null,"abstract":"The total mercury content in 112 fishery products, purchased from Osaka city during 2013-2018, was measured using a heating vaporization mercury analyzer. The average total mercury content in all tuna processed products was found out to be 0.115 μg/g (median=0.070 μg/g). Notably, albacore tuna exhibited the highest mercury content with an average concentration of 0.301 μg/g (median=0.296 μg/g). The total mercury concentration of the fishery products, except processed tuna and Kezuribushi (comprising shavings of dried and smoked mackerel, sardine, as well as horse mackerel), was generally low (<0.1 μg/g).The provisional tolerable weekly intake (PTWI) for mercury as set by the Joint FAO/WHO Expert Committee on Food Additives is 4.0 μg/kg body weight/week. On an average, for Japanese diet, the total weekly mercury intake from fishery products for a person weighing 50 kg was estimated to be 0.13 μg/kg body weight/week, which was 3.3% of the PTWI. These values indicate that it was not a problem to consume fishery products normally. However, albacore tuna cans have a relatively high total mercury concentration; thus, pregnant women consuming them daily may exceed the tolerable weekly intake of methylmercury (2 μg/kg body weight/week) as set by the Food Safety Commission of Japan.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"42 1","pages":"85-91"},"PeriodicalIF":0.3,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86547184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}