肢端藻副鼻藿有毒成分的分离及其化合物的同时分析。

IF 0.2 4区农林科学 Q4 FOOD SCIENCE & TECHNOLOGY

Food Hygiene and Safety Science Pub Date : 2022-04-25 DOI:10.3358/shokueishi.63.79

Y. Ishida, H. Ouchi, T. Kan, H. Shinohara, Katsunori Nakajima, Yuka Nagaoka

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引用次数: 1

摘要

建立了肢端藻副叶柄藻有毒成分丙烯酸A、丙烯酸B和丙烯酸啶的分离方法。采用硅胶、ODS、离子交换柱层析和制备层析等方法对香菇提取物进行了高纯度的纯化。利用这些结果，我们优化了LC-MS/MS条件。最后，我们提出了一种同时分析的方法。加样回收率为80.8 ~ 112.4%，重复性为1.4 ~ 3.8 rsd %。各化合物的定量限为0.25 μg/g。根据实验结果，该方法可以揭示由肢端藻副喙藻引起的食物中毒的原因。

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[Isolating Venomous Constituents of Paralepistopsis acromelalgaand Simultaneous Analysis of Its Compounds].

An isolation method for Acromelic acids A, B and Clitidine, which are venomous constituents of Paralepistopsis acromelalga was developed. Highly purified products were obtained from the mushroom extract using silica gel, ODS, ion-exchange column chromatography and preparative TLC. Using those results, we optimized the LC-MS/MS conditions. Finally we developed a method for simultaneous analysis. In recovery tests, the average recovery was 80.8-112.4%, repeatability was 1.4-3.8RSD%. The limits of quantification of the respective compounds were estimated as 0.25 μg/g. Based on the results, this method can reveal causes of food poisoning by Paralepistopsis acromelalga.

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