Neurophotonics最新文献

{"title":"Potential of plasmonics and nanoscale light-matter interactions for the next generation of optical neural interfaces.","authors":"Filippo Pisano, Liam Collard, Di Zheng, Muhammad Fayyaz Kashif, Mohammadrahim Kazemzadeh, Antonio Balena, Linda Piscopo, Maria Samuela Andriani, Massimo De Vittorio, Ferruccio Pisanello","doi":"10.1117/1.NPh.11.S1.S11513","DOIUrl":"10.1117/1.NPh.11.S1.S11513","url":null,"abstract":"<p><p>Within the realm of optical neural interfaces, the exploration of plasmonic resonances to interact with neural cells has captured increasing attention among the neuroscience community. The interplay of light with conduction electrons in nanometer-sized metallic nanostructures can induce plasmonic resonances, showcasing a versatile capability to both sense and trigger cellular events. We describe the perspective of generating propagating or localized surface plasmon polaritons on the tip of an optical neural implant, widening the possibility for neuroscience labs to explore the potential of plasmonic neural interfaces.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 Suppl 1","pages":"S11513"},"PeriodicalIF":4.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing the path to <i>in-vivo</i> imaging in freely moving mice via multimode-multicore fiber based holographic endoscopy.","authors":"Yang Du, Evelyn Dylda, Miroslav Stibůrek, André D Gomes, Sergey Turtaev, Janelle M P Pakan, Tomáš Čižmár","doi":"10.1117/1.NPh.11.S1.S11506","DOIUrl":"10.1117/1.NPh.11.S1.S11506","url":null,"abstract":"<p><strong>Significance: </strong>Hair-thin multimode optical fiber-based holographic endoscopes have gained considerable interest in modern neuroscience for their ability to achieve cellular and even subcellular resolution during <i>in-vivo</i> deep brain imaging. However, the application of multimode fibers in freely moving animals presents a persistent challenge as it is difficult to maintain optimal imaging performance while the fiber undergoes deformations.</p><p><strong>Aim: </strong>We propose a fiber solution for challenging <i>in-vivo</i> applications with the capability of deep brain high spatial resolution imaging and neuronal activity monitoring in anesthetized as well as awake behaving mice.</p><p><strong>Approach: </strong>We used our previously developed <math><mrow><msup><mi>M</mi><mn>3</mn></msup><mi>CF</mi></mrow></math> multimode-multicore fiber to record fluorescently labeled neurons in anesthetized mice. Our <math><mrow><msup><mi>M</mi><mn>3</mn></msup><mi>CF</mi></mrow></math> exhibits a cascaded refractive index structure, enabling two distinct regimes of light transport that imitate either a multimode or a multicore fiber. The <math><mrow><msup><mi>M</mi><mn>3</mn></msup><mi>CF</mi></mrow></math> has been specifically designed for use in the initial phase of an <i>in-vivo</i> experiment, allowing for the navigation of the endoscope's distal end toward the targeted brain structure. The multicore regime enables the transfer of light to and from each individual neuron within the field of view. For chronic experiments in awake behaving mice, it is crucial to allow for disconnecting the fiber and the animal between experiments. Therefore, we provide here an effective solution and establish a protocol for reconnection of two segments of <math><mrow><msup><mi>M</mi><mn>3</mn></msup><mi>CF</mi></mrow></math> with hexagonally arranged corelets.</p><p><strong>Results: </strong>We successfully utilized the <math><mrow><msup><mi>M</mi><mn>3</mn></msup><mi>CF</mi></mrow></math> to image neurons in anaesthetized transgenic mice expressing enhanced green fluorescent protein. Additionally, we compared imaging results obtained with the <math><mrow><msup><mi>M</mi><mn>3</mn></msup><mi>CF</mi></mrow></math> with larger numerical aperture (NA) fibers in fixed whole-brain tissue.</p><p><strong>Conclusions: </strong>This study focuses on addressing challenges and providing insights into the use of multimode-multicore fibers as imaging solutions for <i>in-vivo</i> applications. We suggest that the upcoming version of the <math><mrow><msup><mi>M</mi><mn>3</mn></msup><mi>CF</mi></mrow></math> increases the overall NA between the two cladding layers to allow for access to high resolution spatial imaging. As the NA increases in the multimode regime, the fiber diameter and ring structure must be reduced to minimize the computational burden and invasiveness.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 Suppl 1","pages":"S11506"},"PeriodicalIF":5.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10863504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139731002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special Section Guest Editorial: Exploiting Complex Media Photonics to Illuminate Brain’s Hidden Depth","authors":"Hana Uhlířová, Tomáš Čižmár, Janelle M. P. Pakan, André Gomes","doi":"10.1117/1.nph.11.s1.s11501","DOIUrl":"https://doi.org/10.1117/1.nph.11.s1.s11501","url":null,"abstract":"The editorial introduces the Neurophotonics Special Issue “Complex Media NeuroPhotonics,” highlighting featured articles.","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"17 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging technologies toward the integration of multiple functionalities on non-planar implantable neurophotonics probes.","authors":"Mohammad Mohammadiaria, Marco Bianco, Antonio Balena, Maria Samuela Andriani, Cinzia Montinaro, Barbara Spagnola, Filippo Pisano, Ferruccio Pisanello, Massimo De Vittorio","doi":"10.1117/1.NPh.11.S1.S11514","DOIUrl":"10.1117/1.NPh.11.S1.S11514","url":null,"abstract":"<p><p>The continuous exchange between the neuroscience and neuroengineering communities that took place over the past decades has uncovered a multitude of technological solutions to interface with the brain. In this framework, a fascinating approach relies on the integration of multiple activation and monitoring capabilities in the same implantable neural probe to better study the multifaceted nature of neural signaling and related functions in the deep brain regions. We highlight current challenges and perspectives on technological developments that could potentially enable the integration of multiple functionalities on optical fiber-based non-planar implantable neurophotonics probes.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 Suppl 1","pages":"S11514"},"PeriodicalIF":4.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11310464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141918109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimode fiber endoscopes for computational brain imaging.","authors":"Lyubov V Amitonova","doi":"10.1117/1.NPh.11.S1.S11509","DOIUrl":"10.1117/1.NPh.11.S1.S11509","url":null,"abstract":"<p><p>Advances in imaging tools have always been a pivotal driver for new discoveries in neuroscience. An ability to visualize neurons and subcellular structures deep within the brain of a freely behaving animal is integral to our understanding of the relationship between neural activity and higher cognitive functions. However, fast high-resolution imaging is limited to sub-surface brain regions and generally requires head fixation of the animal under the microscope. Developing new approaches to address these challenges is critical. The last decades have seen rapid progress in minimally invasive endo-microscopy techniques based on bare optical fibers. A single multimode fiber can be used to penetrate deep into the brain without causing significant damage to the overlying structures and provide high-resolution imaging. Here, we discuss how the full potential of high-speed super-resolution fiber endoscopy can be realized by a holistic approach that combines fiber optics, light shaping, and advanced computational algorithms. The recent progress opens up new avenues for minimally invasive deep brain studies in freely behaving mice.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 Suppl 1","pages":"S11509"},"PeriodicalIF":4.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10917391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140051001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detectability of hemodynamic oscillations in cerebral cortex through functional near-infrared spectroscopy: a simulation study.","authors":"Letizia Contini, Caterina Amendola, Davide Contini, Alessandro Torricelli, Lorenzo Spinelli, Rebecca Re","doi":"10.1117/1.NPh.11.3.035001","DOIUrl":"10.1117/1.NPh.11.3.035001","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Significance: &lt;/strong&gt;We explore the feasibility of using time-domain (TD) and continuous-wave (CW) functional near-infrared spectroscopy (fNIRS) to monitor brain hemodynamic oscillations during resting-state activity in humans, a phenomenon that is of increasing interest in the scientific and medical community and appears to be crucial to advancing the understanding of both healthy and pathological brain functioning.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Aim: &lt;/strong&gt;Our general object is to maximize fNIRS sensitivity to brain resting-state oscillations. More specifically, we aim to define comprehensive guidelines for optimizing main operational parameters in fNIRS measurements [average photon count rate, measurement length, sampling frequency, and source-detector distance (SSD)]. In addition, we compare TD and CW fNIRS performance for the detection and localization of oscillations.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Approach: &lt;/strong&gt;A series of synthetic TD and CW fNIRS signals were generated by exploiting the solution of the diffusion equation for two different geometries of the probed medium: a homogeneous medium and a bilayer medium. Known and periodical perturbations of the concentrations of oxy- and deoxy-hemoglobin were imposed in the medium, determining changes in its optical properties. The homogeneous slab model was used to determine the effect of multiple measurement parameters on fNIRS sensitivity to oscillatory phenomena, and the bilayer model was used to evaluate and compare the abilities of TD and CW fNIRS in detecting and isolating oscillations occurring at different depths. For TD fNIRS, two approaches to enhance depth-selectivity were evaluated: first, a time-windowing of the photon distribution of time-of-flight was performed, and then, the time-dependent mean partial pathlength (TMPP) method was used to retrieve the hemoglobin concentrations in the medium.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;In the homogeneous medium case, the sensitivity of TD and CW fNIRS to periodical perturbations of the optical properties increases proportionally with the average photon count rate, the measurement length, and the sampling frequency and approximatively with the square of the SSD. In the bilayer medium case, the time-windowing method can detect and correctly localize the presence of oscillatory components in the TD fNIRS signal, even in the presence of very low photon count rates. The TMPP method demonstrates how to correctly retrieve the periodical variation of hemoglobin at different depths from the TD fNIRS signal acquired at a single SSD. For CW fNIRS, measurements taken at typical SSDs used for short-separation channel regression show notable sensitivity to oscillations occurring in the deep layer, challenging the assumptions underlying this correction method when the focus is on analyzing oscillatory phenomena.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;We demonstrated that the TD fNIRS technique allows for the detection and depth-localization of periodical fluctuations of ","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 3","pages":"035001"},"PeriodicalIF":4.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11221108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Closed-loop experiments and brain machine interfaces with multiphoton microscopy.","authors":"Riichiro Hira","doi":"10.1117/1.NPh.11.3.033405","DOIUrl":"10.1117/1.NPh.11.3.033405","url":null,"abstract":"<p><p>In the field of neuroscience, the importance of constructing closed-loop experimental systems has increased in conjunction with technological advances in measuring and controlling neural activity in live animals. We provide an overview of recent technological advances in the field, focusing on closed-loop experimental systems where multiphoton microscopy-the only method capable of recording and controlling targeted population activity of neurons at a single-cell resolution <i>in vivo</i>-works through real-time feedback. Specifically, we present some examples of brain machine interfaces (BMIs) using <i>in vivo</i> two-photon calcium imaging and discuss applications of two-photon optogenetic stimulation and adaptive optics to real-time BMIs. We also consider conditions for realizing future optical BMIs at the synaptic level, and their possible roles in understanding the computational principles of the brain.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 3","pages":"033405"},"PeriodicalIF":5.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10876015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139906929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Never bored!","authors":"Anna Devor","doi":"10.1117/1.NPh.11.3.030101","DOIUrl":"10.1117/1.NPh.11.3.030101","url":null,"abstract":"<p><p>Neurophotonics Editor-in-Chief Anna Devor reflects on the wonderful feeling of inspiration in the neurophotonic community.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 3","pages":"030101"},"PeriodicalIF":4.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11440177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142332384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Encoding of self-initiated actions in axon terminals of the mesocortical pathway.","authors":"Makoto Ohtake, Kenta Abe, Masashi Hasegawa, Takahide Itokazu, Vihashini Selvakumar, Ashley Matunis, Emma Stacy, Emily Froeblich, Nathan Huynh, Haesuk Lee, Yuki Kambe, Tetsuya Yamamoto, Tatsuo K Sato, Takashi R Sato","doi":"10.1117/1.NPh.11.3.033408","DOIUrl":"10.1117/1.NPh.11.3.033408","url":null,"abstract":"<p><strong>Significance: </strong>The initiation of goal-directed actions is a complex process involving the medial prefrontal cortex and dopaminergic inputs through the mesocortical pathway. However, it is unclear what information the mesocortical pathway conveys and how it impacts action initiation. In this study, we unveiled the indispensable role of mesocortical axon terminals in encoding the execution of movements in self-initiated actions.</p><p><strong>Aim: </strong>To investigate the role of mesocortical axon terminals in encoding the execution of movements in self-initiated actions.</p><p><strong>Approach: </strong>We designed a lever-press task in which mice internally determine the timing of the press, receiving a larger reward for longer waiting periods.</p><p><strong>Results: </strong>Our study revealed that self-initiated actions depend on dopaminergic signaling mediated by D2 receptors, whereas sensory-triggered lever-press actions do not involve D2 signaling. Microprism-mediated two-photon calcium imaging further demonstrated ramping activity in mesocortical axon terminals approximately 0.5 s before the self-initiated lever press. Remarkably, the ramping patterns remained consistent whether the mice responded to cues immediately for a smaller reward or held their response for a larger reward.</p><p><strong>Conclusions: </strong>We conclude that mesocortical dopamine axon terminals encode the timing of self-initiated actions, shedding light on a crucial aspect of the intricate neural mechanisms governing goal-directed behavior.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 3","pages":"033408"},"PeriodicalIF":5.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11080647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Widefield <i>in vivo</i> imaging system with two fluorescence and two reflectance channels, a single sCMOS detector, and shielded illumination.","authors":"Patrick R Doran, Natalie Fomin-Thunemann, Rockwell P Tang, Dora Balog, Bernhard Zimmerman, Kıvılcım Kılıç, Emily A Martin, Sreekanth Kura, Harrison P Fisher, Grace Chabbott, Joel Herbert, Bradley C Rauscher, John X Jiang, Sava Sakadzic, David A Boas, Anna Devor, Ichun Anderson Chen, Martin Thunemann","doi":"10.1117/1.NPh.11.3.034310","DOIUrl":"10.1117/1.NPh.11.3.034310","url":null,"abstract":"<p><strong>Significance: </strong>Widefield microscopy of the entire dorsal part of mouse cerebral cortex enables large-scale (\"mesoscopic\") imaging of different aspects of neuronal activity with spectrally compatible fluorescent indicators as well as hemodynamics via oxy- and deoxyhemoglobin absorption. Versatile and cost-effective imaging systems are needed for large-scale, color-multiplexed imaging of multiple fluorescent and intrinsic contrasts.</p><p><strong>Aim: </strong>We aim to develop a system for mesoscopic imaging of two fluorescent and two reflectance channels.</p><p><strong>Approach: </strong>Excitation of red and green fluorescence is achieved through epi-illumination. Hemoglobin absorption imaging is achieved using 525- and 625-nm light-emitting diodes positioned around the objective lens. An aluminum hemisphere placed between objective and cranial window provides diffuse illumination of the brain. Signals are recorded sequentially by a single sCMOS detector.</p><p><strong>Results: </strong>We demonstrate the performance of our imaging system by recording large-scale spontaneous and stimulus-evoked neuronal, cholinergic, and hemodynamic activity in awake, head-fixed mice with a curved \"crystal skull\" window expressing the red calcium indicator jRGECO1a and the green acetylcholine sensor <math> <mrow> <msub><mrow><mi>GRAB</mi></mrow> <mrow><mi>ACh</mi> <mn>3.0</mn></mrow> </msub> </mrow> </math> . Shielding of illumination light through the aluminum hemisphere enables concurrent recording of pupil diameter changes.</p><p><strong>Conclusions: </strong>Our widefield microscope design with a single camera can be used to acquire multiple aspects of brain physiology and is compatible with behavioral readouts of pupil diameter.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 3","pages":"034310"},"PeriodicalIF":4.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11177117/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141332486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}