Archives of Endocrinology Metabolism最新文献_第2页

Pathogenicity of germline VHL variants is associated with renal cell carcinoma size in von Hippel-Lindau disease.

IF 1.6 4区医学

Archives of Endocrinology Metabolism Pub Date : 2025-02-13 DOI: 10.20945/2359-4292-2024-0354

Gustavo H Mori, Gustavo F C Fagundes, Lucas S Santana, Felipe Freitas-Castro, Ana Caroline F Afonso, Delmar M Lourenço, Maria Adelaide A Pereira, Fabio Y Tanno, Victor Srougi, Jose L Chambo, Mauricio D Cordeiro, William C Nahas, Ana O Hoff, Maria Candida B V Fragoso, Berenice B Mendonca, Ana Claudia Latronico, Madson Q Almeida

{"title":"Pathogenicity of germline VHL variants is associated with renal cell carcinoma size in von Hippel-Lindau disease.","authors":"Gustavo H Mori, Gustavo F C Fagundes, Lucas S Santana, Felipe Freitas-Castro, Ana Caroline F Afonso, Delmar M Lourenço, Maria Adelaide A Pereira, Fabio Y Tanno, Victor Srougi, Jose L Chambo, Mauricio D Cordeiro, William C Nahas, Ana O Hoff, Maria Candida B V Fragoso, Berenice B Mendonca, Ana Claudia Latronico, Madson Q Almeida","doi":"10.20945/2359-4292-2024-0354","DOIUrl":"10.20945/2359-4292-2024-0354","url":null,"abstract":"Objective: In this study, our aim was to search for new genotype-phenotype correlations in patients with Von Hippel-Lindau (VHL) disease.Subjects and methods: We retrospectively studied 53 consecutive patients with VHL disease and confirmed genetic diagnoses from 32 relatives.Results: Most VHL pathogenic or likely pathogenic variants were missense (18 out of 32; 56.25%). The median size of the large carcinoma (RCC) was 3.6 cm (interquartile range, 2.8 to 6.5 cm). Interestingly, the size of the large RCC in patients harboring VHL pathogenic variants (n = 9) was significantly greater than that in patients with VHL likely pathogenic (n = 7) variants (5.4 cm [3.65 to 6.6] vs. 2.9 cm [2.45 to 3.35]; p = 0.008). Moreover, adrenal paraganglioma (PGL) (82.35% vs. 17.65%; p = 0.0001) and pancreatic neuroendocrine tumor (PNET) (81.81% vs. 18.18%; p = 0.007) were associated with missense VHL pathogenic or likely pathogenic variants compared with non-missense defects. In contrast, central nervous system (CNS) hemangioblastomas (HBs) (90.47% vs. 53.12%; p = 0.004), pancreatic cysts (76.19% vs. 28.12%; p = 0.001) and RCCs (57.14% vs. 12.5; p = 0.001) were more common in patients with non-missense VHL variants.Conclusion: VHL pathogenic variants were associated with larger RCCs than were VHL likely pathogenic variants.","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e240354"},"PeriodicalIF":1.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143410701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Audiological alterations in resistance to thyroid hormone syndrome: emphasizing lifelong assessment.

IF 1.6 4区医学

Archives of Endocrinology Metabolism Pub Date : 2025-02-13 DOI: 10.20945/2359-4292-2024-0078

Alexandre Machado Silva de Oliveira, Viviane Motti, Jessica Casemiro, Pedro Resende Rende, Caio Leônidas Oliveira de Andrade, Luciene da Cruz Fernande, Helton Estrela Ramos

{"title":"Audiological alterations in resistance to thyroid hormone syndrome: emphasizing lifelong assessment.","authors":"Alexandre Machado Silva de Oliveira, Viviane Motti, Jessica Casemiro, Pedro Resende Rende, Caio Leônidas Oliveira de Andrade, Luciene da Cruz Fernande, Helton Estrela Ramos","doi":"10.20945/2359-4292-2024-0078","DOIUrl":"10.20945/2359-4292-2024-0078","url":null,"abstract":"The aim of this study was to investigate the long-term audiological consequences of resistance to thyroid hormone (RTH) syndrome. The cochlea and inner ear express thyroid hormone receptor beta (THRB) in developmental stages. Hearing loss is frequent in subjects with RTH syndrome; however, the long-term impact of insufficient thyroid hormone action in the auditory system remains unknown. Subjects with RTH from the same family, carrying a THRB gene variant, underwent detailed clinical evaluation and serum biochemistry analysis. The genetic assessment involved sequencing of the THRB gene. Hearing loss assessment included (i) meatoscopy, (ii) audiometric tests using pure tone audiometry, (iii) middle ear evaluation by tympanometry, (iv) transient otoacoustic emissions (TOAE), and (v) computed tomography of the mastoids. Genetic sequencing confirmed the THRB gene alteration (p.M442T) in three family members. All affected subjects had clinical and laboratory RTH features. Notably, the older subject with RTH was affected by a bilateral sensorineural hearing loss pattern affected by high frequencies, and cochlear dysfunction was also presented by TOAE analysis, indicating pronounced hearing loss. Hearing loss represents a significant concern in subjects with RTH, emphasizing the need for continuous and comprehensive audiological assessments. These findings underscore the importance of lifelong monitoring, particularly to assess age-related progression of hearing impairment.","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e240078"},"PeriodicalIF":1.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does Radiofrequency Echographic Multi-Spectrometry (REMS) perform similarly to Dual-energy X-ray Absorptiometry (DXA) in terms of Trabecular Bone Score (TBS) and FRAX?

IF 1.6 4区医学

Archives of Endocrinology Metabolism Pub Date : 2025-02-13 DOI: 10.20945/2359-4292-2023-0456

Débora Meira Ramos Amorim, Eliane Naomi Sakane, Sergio Setsuo Maeda, Marise Lazaretti-Castro

{"title":"Does Radiofrequency Echographic Multi-Spectrometry (REMS) perform similarly to Dual-energy X-ray Absorptiometry (DXA) in terms of Trabecular Bone Score (TBS) and FRAX?","authors":"Débora Meira Ramos Amorim, Eliane Naomi Sakane, Sergio Setsuo Maeda, Marise Lazaretti-Castro","doi":"10.20945/2359-4292-2023-0456","DOIUrl":"10.20945/2359-4292-2023-0456","url":null,"abstract":"Objective: To evaluate whether bone assessment by radiofrequency echographic multi-spectrometry (REMS) is influenced by trabecular bone integrity by comparing it to dual-energy X-ray absorptiometry (DXA) and the trabecular bone score (TBS). Additionally, the study aims to determine if comparing fracture risk using FRAX and the National Osteoporosis Guideline Group (NOGG) using the T-score from each method would lead to differences in a Brazilian female population.Subjects and methods: A sample of women aged 30-80 underwent REMS and DXA scans of axial sites at the Hospital São Paulo, Brazil. Subsequently, TBS was applied to DXA exams. Clinical data were obtained from hospital records and phone interviews to calculate fracture risk.Results: Among the 343 participants enrolled, 213 had comparable lumbar spine exams by REMS, DXA, and TBS, and 166 had comparable hip exams by REMS and DXA. The correlation between lumbar spine bone mineral density (BMD) by REMS and the TBS was low (r = 0.27, p < 0.001), as was the correlation between DXA and TBS (r = 0.39, p < 0.001). No statistically significant difference was found between the TBS classifications of osteoporotic lumbar spine by REMS and DXA (p = 0.178). Fracture risk data by FRAX were obtained from 119 participants, with 92% receiving concordant NOGG classifications for major osteoporotic fracture risk from REMS and DXA (κ = 0.71 CI95% (0.54 to 0.89), p < 0.001), and 87% for hip fracture risk (κ = 0.58 CI95% (0.38 to 0.77), p < 0.001).Conclusion: REMS performed similarly to DXA in assessing trabecular integrity using TBS. Additionally, no statistically significant difference was observed in fracture risk assessment by FRAX based on NOGG recommendations.","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e230456"},"PeriodicalIF":1.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A paradigm shift in nutritional clinical practice: filling a gap on the implementation of the Dietary Guidelines for the Brazilian Population.

IF 1.6 4区医学

Archives of Endocrinology Metabolism Pub Date : 2025-02-13 DOI: 10.20945/2359-4292-2024-0446

Kamila Tiemann Gabe, Carlos Augusto Monteiro

引用次数: 0

Microscopic testicular sperm extraction in 46, XY differences in sex development caused by 5-alpha reductase type 2 deficiency.

IF 1.6 4区医学

Archives of Endocrinology Metabolism Pub Date : 2025-02-13 DOI: 10.20945/2359-4292-2024-0223

Joao Paulo Greco Cardoso, Berenice Bilharinho Mendonça, William Carlos Nahas, Marcello Antonio Signorelli Cocuzza

{"title":"Microscopic testicular sperm extraction in 46, XY differences in sex development caused by 5-alpha reductase type 2 deficiency.","authors":"Joao Paulo Greco Cardoso, Berenice Bilharinho Mendonça, William Carlos Nahas, Marcello Antonio Signorelli Cocuzza","doi":"10.20945/2359-4292-2024-0223","DOIUrl":"10.20945/2359-4292-2024-0223","url":null,"abstract":"The 46, XY differences in sex development (DSD) caused by 5-alpha reductase type 2 (5ARD2) often presents with bilateral undescended testicles, otherwise normal internal reproductive structures, prostate hypoplasia and undervirilized male genitalia. Notably, as one of the few DSDs where fertility is possible, the clinical presentation of this disease is diverse, and reported cases of assisted reproduction are scarce. The fertility potential, reproductive counseling and treatment depend on the clinical presentation of this DSD, especially the testicular position and urethral anatomy. The influence of the timing and modality of surgery for hypospadias and cryptorchidism should be considered. We aimed to describe the use of microscopic testicular sperm extraction (micro-TESE) in this population. We provide a descriptive analysis of how micro-TESE is a possible potential tool for assisted reproduction in 5ARD2-deficient patients. A 33-year-old male who underwent bilateral orchidopexy, phalloplasty, and urethroplasty at the age of 9 years presented successful sperm retrieval but failed embryo development after intracytoplasmic sperm injection. Testicular histology revealed late spermatogenic arrest. A 28-year-old male with bilateral orchidopexy, phalloplasty, and urethroplasty at age 25 with unsuccessful sperm retrieval. Testicular histology revealed a Sertoli cell-only pattern. 5ARD2-deficient patients are singular patients. The potential impact of the time between atypical genitalia procedures and orchidopexy on fertility should be highly considered. Micro-TESE is a technique that may be used to assist azoospermic patients in this population. Early orchidopexy and penile and urethral corrections should be considered key strategies to preserve the fertility potential of 5ARD2 patients.","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e240223"},"PeriodicalIF":1.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Translating the Brazilian Dietary Guidelines into clinical practice: innovative strategies for healthcare professionals.

IF 1.6 4区医学

Archives of Endocrinology Metabolism Pub Date : 2025-02-03 DOI: 10.20945/2359-4292-2024-0142

Vanessa Del Castillo Silva Couto, Maria Laura da Costa Louzada, Patrícia Constante Jaime

{"title":"Translating the Brazilian Dietary Guidelines into clinical practice: innovative strategies for healthcare professionals.","authors":"Vanessa Del Castillo Silva Couto, Maria Laura da Costa Louzada, Patrícia Constante Jaime","doi":"10.20945/2359-4292-2024-0142","DOIUrl":"10.20945/2359-4292-2024-0142","url":null,"abstract":"The Brazilian Dietary Guidelines provide crucial recommendations for a healthy diet, aiming at promoting health and preventing non-communicable chronic diseases. The core principle is the preference for natural or minimally processed foods and freshly prepared dishes over ultra-processed foods. Despite their growing recognition, healthcare professionals struggle to integrate these guidelines into clinical practice. This article aims to present two innovative strategies for incorporating the Brazilian Dietary Guidelines into healthcare. The Protocols based on the Brazilian Dietary Guidelines for Individual Dietary Advice are standardized clinical tools to support healthcare professionals (nutritionists or not) in giving nutritional advice during individual appointments to various life stages. The Protocols operationalize the assessment of individuals' dietary patterns using the Food Consumption Markers Questionnaire and support the delivery of personalized and priority recommendations through a stepwise flowchart. Conversely, Brazilian Dietary Guidelines-based Meal Plans consist of personalized dietary prescriptions comprising structured daily menus that, unlike conventional plans primarily focusing on nutrient goals, prioritize overall eating patterns guided by the Brazilian Dietary Guidelines. The proposal encourages, in the first place, the selection of a variety of culinary preparations based on natural or minimally processed foods, emphasizing tasteful, accessible, and culturally appropriate choices as the initial step. In a second step, these plans can be customized to individual energy requirements, and adjustments made based on strategic nutrient needs. This article aims to support the enhancement of healthcare professionals' skills in promoting healthy eating practices, thereby contributing to improved health and a reduced disease burden among the Brazilian population.","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e240142"},"PeriodicalIF":1.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacologic Treatment of Obesity in adults and its impact on comorbidities: 2024 Update and Position Statement of Specialists from the Brazilian Association for the Study of Obesity and Metabolic Syndrome (Abeso) and the Brazilian Society of Endocrinology and Metabolism (SBEM). 成人肥胖的药物治疗及其对合并症的影响：巴西肥胖与代谢综合征研究协会（Abeso）和巴西内分泌与代谢学会（SBEM）专家的2024年更新和立场声明。

IF 1.6 4区医学

Archives of Endocrinology Metabolism Pub Date : 2024-11-25 eCollection Date: 2024-01-01 DOI: 10.20945/2359-4292-2024-0422

Rodrigo O Moreira, Cynthia M Valerio, Alexandre Hohl, Cristiane Moulin, Fábio Moura, Fábio R Trujilho, Fernando Gerchman, Livia L Correa, Marcio C Mancini, Maria Edna Melo, Rodrigo N Lamounier, Simone van de Sande-Lee, Thaísa D G Trujilho, Paulo A C Miranda, Bruno Halpern

{"title":"Pharmacologic Treatment of Obesity in adults and its impact on comorbidities: 2024 Update and Position Statement of Specialists from the Brazilian Association for the Study of Obesity and Metabolic Syndrome (Abeso) and the Brazilian Society of Endocrinology and Metabolism (SBEM).","authors":"Rodrigo O Moreira, Cynthia M Valerio, Alexandre Hohl, Cristiane Moulin, Fábio Moura, Fábio R Trujilho, Fernando Gerchman, Livia L Correa, Marcio C Mancini, Maria Edna Melo, Rodrigo N Lamounier, Simone van de Sande-Lee, Thaísa D G Trujilho, Paulo A C Miranda, Bruno Halpern","doi":"10.20945/2359-4292-2024-0422","DOIUrl":"10.20945/2359-4292-2024-0422","url":null,"abstract":"Pharmacological treatment of obesity is passing through many changes in the last decades; different agents have been approved, and newer options are leaning towards higher efficacy and a more favourable safety profile; however, medications approved for a longer time are still available and useful for many patients. This document is an 2024 Update Position Statement of Specialists from the Brazilian Association for the Study of Obesity and Metabolic Syndrome (Abeso) and the Brazilian Society of Endocrinology and Metabolism (SBEM), with the aim of reviewing all the approved medications for the management of obesity in Brazil (sibutramine, orlistat, liraglutide, semaglutide and bupropion/naltrexone fixed dose), with the addition of tirzepatide, that is approved in other countries and likely approved soon in Brazil. The review is focused on efficacy, safety profile and the impact of drugs (based on existing studies) on different comorbidities.","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"68 ","pages":"e240422"},"PeriodicalIF":1.6,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11634287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of tributyltin on placental and reproductive abnormalities in offspring.

IF 1.6 4区医学

Archives of Endocrinology Metabolism Pub Date : 2024-11-06 eCollection Date: 2024-01-01 DOI: 10.20945/2359-4292-2024-0186

Charles S da Costa, Hanin Alahmadi, Genoa R Warner, Maria Tereza Nunes, Glaecir Roseni Mundstock Dias, Leandro Miranda-Alves, Jones B Graceli

引用次数: 0

Association between the aromatase (CYP19A1) gene variant rs10046 and cardiovascular risk in postmenopausal women.

IF 1.6 4区医学

Archives of Endocrinology Metabolism Pub Date : 2024-11-06 eCollection Date: 2024-01-01 DOI: 10.20945/2359-4292-2024-0087

Betânia Rodrigues Dos Santos, Gislaine Casanova, Thais Rasia da Silva, Karen Oppermann, Poli Mara Spritzer

{"title":"Association between the aromatase (CYP19A1) gene variant rs10046 and cardiovascular risk in postmenopausal women.","authors":"Betânia Rodrigues Dos Santos, Gislaine Casanova, Thais Rasia da Silva, Karen Oppermann, Poli Mara Spritzer","doi":"10.20945/2359-4292-2024-0087","DOIUrl":"10.20945/2359-4292-2024-0087","url":null,"abstract":"Objective: To assess the genotypic and allelic distribution of the rs10046 polymorphism in the CYP19A1 gene and evaluate whether this aromatase gene variant is associated with cardiovascular risk in postmenopausal women.Materials and methods: This cross-sectional study analyzed repository-stored samples from 370 postmenopausal women aged 44-72 years. Clinical, metabolic, and hormonal data were collected. The patients' estimated 10-year atherosclerotic cardiovascular disease (ASCVD) risk was calculated using the ASCVD Risk Estimator Plus, as recommended by the American College of Cardiology/American Heart Association. Genotyping of the rs10046 polymorphism of the CYP19A1 gene was carried out using real-time polymerase chain reaction with allelic discrimination assays.Results: The participants had a mean age of 56.07 ± 5.58 years and a mean body mass index (BMI) of 27.73 ± 5.41 kg/m². The 10-year ASCVD risk was estimated to be low, borderline, intermediate, and high in 64.7%, 12.8%, 19.8%, and 2.7% of the participants, respectively. The CC genotype of the rs10046 polymorphism was associated with low estradiol levels (p = 0.003) and high ASCVD scores (p = 0.014). In a multivariate model, age (p < 0.001) and CC genotype (p = 0.021) were independently associated with higher ASCVD risk.Conclusion: The present study found that the CC genotype of the rs10046 polymorphism in the CYP19A1 gene is associated with low estradiol levels and an increased ASCVD risk. Additionally, the results indicated that, among postmenopausal women, age and the CC genotype of rs10046 were associated with a high prevalence of ASCVD risk, independent of BMI.","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"68 Spec","pages":"e240087"},"PeriodicalIF":1.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143061513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prenatal exposure to nitrate alters uterine morphology and gene expression in adult female F1 generation rats.

IF 1.6 4区医学

Archives of Endocrinology Metabolism Pub Date : 2024-11-06 eCollection Date: 2024-01-01 DOI: 10.20945/2359-4292-2024-0085

André Gilberto Cassiani, Thiago Pinheiro Arrais Aloia, Érica Kássia Sousa-Vidal, Sérgio Podgaec, Carla de Azevedo Piccinato, Caroline Serrano-Nascimento

{"title":"Prenatal exposure to nitrate alters uterine morphology and gene expression in adult female F1 generation rats.","authors":"André Gilberto Cassiani, Thiago Pinheiro Arrais Aloia, Érica Kássia Sousa-Vidal, Sérgio Podgaec, Carla de Azevedo Piccinato, Caroline Serrano-Nascimento","doi":"10.20945/2359-4292-2024-0085","DOIUrl":"10.20945/2359-4292-2024-0085","url":null,"abstract":"Objective: Nitrate is ubiquitously found in the environment and is one of the main components of nitrogen fertilizers. Previous studies have shown that nitrate disrupts the reproductive system in aquatic animals, but no study has evaluated the impact of nitrate exposure on the uterus in mammals. This study aimed to evaluate the impact of maternal exposure to nitrate during the prenatal period on uterine morphology and gene expression in adult female F1 rats.Materials and methods: Pregnant Wistar rats were either treated with sodium nitrate 20 mg/L or 50 mg/L dissolved in drinking water from the first day of pregnancy until the birth of the offspring or were left untreated. On postnatal day 90, the uteri of female offspring rats were collected for histological and gene expression analyses. Morphometric analyses of the uterine photomicrographs were performed to determine the thickness of the layers of the uterine wall (endometrium, myometrium, and perimetrium) and the number of endometrial glands.Results: The highest nitrate dose increased the myometrial thickness of the exposed female rats. Treatment with both nitrate doses reduced the number of endometrial glands compared with no treatment. Additionally, nitrate treatment significantly increased the expression of estrogen receptors and reduced the expression of progesterone receptors in the uterus.Conclusion: Our results strongly suggest that prenatal exposure to nitrate programs gene expression and alters the uterine morphology in female F1 rats, potentially increasing their susceptibility to developing uterine diseases during adulthood.","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"68 Spec","pages":"e240085"},"PeriodicalIF":1.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143061055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0