Archives of Endocrinology Metabolism最新文献

{"title":"MAP17 contributes to the tumorigenesis of papillary thyroid carcinoma by activating the AKT signaling pathway.","authors":"Zhen-Hua Tian, Rui Huang, Gang-Qiang Li, Yong-Xue Zhu","doi":"10.20945/2359-4292-2024-0342","DOIUrl":"10.20945/2359-4292-2024-0342","url":null,"abstract":"<p><strong>Objective: </strong>This study investigates the role of membrane-associated protein 17 (MAP17) and the Akt signaling pathway in the progression of papillary thyroid carcinoma (PTC).</p><p><strong>Materials and methods: </strong>We conducted a series of in vitro experiments using PTC cell lines (HTori-3 and TPC-1). Cells were divided into three groups: control, MAP17 inhibitor negative control (NC), and MAP17 inhibitor treatment. Cell viability was assessed at 0, 24, 48, and 72 hours using the Cell Counting Kit-8 (CCK-8) assay. Apoptosis levels were measured by flow cytometry, and protein and mRNA expression of MAP17, phosphorylated Akt (p-AKT), and Akt were analyzed by Western blot and qRT-PCR.</p><p><strong>Results: </strong>Cell viability in the control, MAP17 inhibitor NC, and MAP17 inhibitor groups increased significantly over time (P < 0.05). Notably, in both HTori-3 and TPC-1 cells, the MAP17 inhibitor significantly reduced cell viability compared to the control and NC groups at 24, 48, and 72 hours (P < 0.05). Furthermore, apoptosis levels were significantly higher in the MAP17 inhibitor group compared to the control and NC groups (P < 0.05). Western blot and qRT-PCR analyses revealed that MAP17 and p-Akt protein and mRNA levels were significantly higher in the control and NC groups compared to the MAP17 inhibitor group (P < 0.05). However, no significant differences in total Akt protein or mRNA levels were observed across groups.</p><p><strong>Conclusion: </strong>Our findings suggest that MAP17 and the Akt signaling pathway play a crucial role in promoting the progression of PTC. Inhibition of MAP17 suppresses cell viability and induces apoptosis, indicating that MAP17 may be a promising therapeutic target for PTC. The data also highlight the potential for targeting the MAP17-Akt axis in developing future treatments for PTC.</p>","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e240342"},"PeriodicalIF":1.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11895518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive microarray analysis for the identification of therapeutic targets within HIF-1α signalling networks in diet-induced obesity via hypothalamic inflammation.","authors":"Hai Guo, Lijuan Ma, Dilihumaier Duolikun, Qiaoling Yao","doi":"10.20945/2359-4292-2024-0098","DOIUrl":"10.20945/2359-4292-2024-0098","url":null,"abstract":"<p><strong>Objective: </strong>A high-fat diet (HFD) significantly contributes to obesity and alters the neurological function of the brain. This study explored the influence of hypoxia-inducible factor (HIF-1) and its downstream molecules on obesity progression in the context of HFD-induced hypothalamic inflammation.</p><p><strong>Materials and methods: </strong>Utilizing a bioinformatics approach alongside animal models, targets and pathways related to hypothalamic obesity were identified via network analysis, gene target identification, gene ontology analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and subsequent validation in animal models.</p><p><strong>Results: </strong>HIF-1α has the potential to regulate the immune response by promoting immune infiltration and increasing the population of immune cells, particularly memory CD4 T cells, in the hypothalamus, primarily through its influence on ksr2 expression. Additionally, the analysis predicted five drugs capable of enhancing HIF-1-Ksr2 signalling.</p><p><strong>Conclusion: </strong>In conclusion, targeting Ksr2 with specific drugs represents a potential approach for addressing HFD-induced obesity. These novel findings lay the groundwork for developing dietary supplements and therapeutic interventions.</p>","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e240098"},"PeriodicalIF":1.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11895521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to the comment on \"Diagnostic value of a computer-assisted diagnosis system for the ultrasound features in thyroid nodules\".","authors":"Yiwei Wang","doi":"10.20945/2359-4292-2024-0526","DOIUrl":"10.20945/2359-4292-2024-0526","url":null,"abstract":"","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e240526"},"PeriodicalIF":1.6,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143517439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on the \"Diagnostic value of a computer- -assisted diagnosis system for the ultrasound features in thyroid nodules\".","authors":"Ahmet Bozer","doi":"10.20945/2359-4292-2024-0419","DOIUrl":"10.20945/2359-4292-2024-0419","url":null,"abstract":"","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e240419"},"PeriodicalIF":1.6,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143517436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium nutritional status and thyroid dysfunction.","authors":"Luciana Sant'Ana Leone de Souza, Renata de Oliveira Campos, Jair de Souza Braga, Joice Dos Santos de Jesus, Helton Estrela Ramos, Sara Moreira Anunciação, Jéssica Fernanda Cassemiro, Pedro Resende Ferreira Rende, Fábio Hecht","doi":"10.20945/2359-4292-2023-0348","DOIUrl":"10.20945/2359-4292-2023-0348","url":null,"abstract":"<p><p>Selenium(Se) is an essential micronutrient for several immune and regulatory functions in the body. In thyroid tissue, Se contributes to the antioxidant system and is a crucial component of deiodinases, which are selenoproteins that participate in thyroid hormone metabolism. Additionally, this micronutrient exerts a significant impact on thyroid pathophysiology, as low levels of Se lead to reduced activity of glutathione peroxidase, a selenoprotein involved in antioxidative processes, thereby resulting in increased oxidative stress and damage to thyroid tissue. Selenium deficiency (SeD) can cause growth retardation and reproductive failure; in women and children, it may result in Keshan's disease and Kashin-Beck's disease. Research has shown an inverse correlation between Se serum levels and autoimmune thyroiditis in areas with mild SeD. In Graves' disease, Se supplementation has been linked to faster achievement of euthyroidism as well as improvements in quality of life, lessened orbital involvement, and slower ocular progression of the disease. Furthermore, several studies suggest an association between serum SeD and the development of thyroid cancer. Maintaining physiological Se concentrations appears to be related to the prevention of thyroid disease, although current data are insufficient to conclusively support or refute the efficacy of supplementation. Through this narrative review, we aim to present the latest information on the role of selenium in thyroid pathophysiology. To identify relevant literature, specific search strategies were employed in the electronic databases PubMed, Lilacs, and SciELO.</p>","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e230348"},"PeriodicalIF":1.6,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11849045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adult height of children born small for gestational age treated with growth hormone and gonadotropin-releasing hormone analogs in Southern Brazil.","authors":"Luís Eduardo Cruvinel Pinto, Adriane de Andre Cardoso-Demartini, Julienne Angela Ramires de Carvalho, Gabriela de Carvalho Kraemer, Rosana Marques Pereira, Aline Scheidemantel, Gabriel Junqueira Soares, Suzana Nesi-França","doi":"10.20945/2359-4292-2023-0513","DOIUrl":"10.20945/2359-4292-2023-0513","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate adult height and identify the factors contributing to its achievement in patients born small for gestational age (SGA) treated with recombinant human growth hormone (rhGH).</p><p><strong>Subjects and methods: </strong>This retrospective study includes data of SGA children treated at a pediatric endocrinology center. Inclusion criteria were SGA birth (birth length and/or weight < -1.28 standard deviation score (SDS), absence of catch-up growth by the age of four years, rhGH treatment for more than 12 months, and recorded adult height. Birth size SDS was calculated using Intergrowth-21st (gestational age ≥ 33 weeks) or Fenton (<33 weeks) standards. Patients with uncontrolled chronic diseases, genetic syndromes, or growth hormone deficiency were excluded. An increase of 0.6 SDS or more was considered a positive response.</p><p><strong>Results: </strong>Twenty-four patients (14 boys) were included, with an average gestational age of 38.0 (range: 33.0-40.0) weeks, birth weight of -1.3 ± 0.9 SDS, and birth length of -2.4 ± 0.7 SDS. They were treated with rhGH starting at an average age of 10.3 ± 2.6 years for a duration of 5.4 ± 2.3 years. Height SDS increased from -2.6 ± 0.4 SDS to -1.2 ± 0.6 SDS, which was comparable to the target height SDS (-1.3 ± 0.9; p = 0.3). Although 18 children were classified as good responders, 6 did not achieve a final height SDS > -2.0. Adult height was correlated with the increment in height SDS and growth velocity during the first year of treatment. No significant differences were observed between children classified as SGA by birth weight or length < 10th percentile and those by weight or length < -2.0 SDS.</p><p><strong>Conclusion: </strong>In this cohort of children born SGA with short stature, rhGH treatment effectively improved adult height. Given the diverse causes of being born SGA, the response to rhGH therapy may vary.</p>","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e230513"},"PeriodicalIF":1.6,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11849044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenicity of germline VHL variants is associated with renal cell carcinoma size in von Hippel-Lindau disease.","authors":"Gustavo H Mori, Gustavo F C Fagundes, Lucas S Santana, Felipe Freitas-Castro, Ana Caroline F Afonso, Delmar M Lourenço, Maria Adelaide A Pereira, Fabio Y Tanno, Victor Srougi, Jose L Chambo, Mauricio D Cordeiro, William C Nahas, Ana O Hoff, Maria Candida B V Fragoso, Berenice B Mendonca, Ana Claudia Latronico, Madson Q Almeida","doi":"10.20945/2359-4292-2024-0354","DOIUrl":"10.20945/2359-4292-2024-0354","url":null,"abstract":"<p><strong>Objective: </strong>In this study, our aim was to search for new genotype-phenotype correlations in patients with Von Hippel-Lindau (VHL) disease.</p><p><strong>Subjects and methods: </strong>We retrospectively studied 53 consecutive patients with VHL disease and confirmed genetic diagnoses from 32 relatives.</p><p><strong>Results: </strong>Most VHL pathogenic or likely pathogenic variants were missense (18 out of 32; 56.25%). The median size of the large carcinoma (RCC) was 3.6 cm (interquartile range, 2.8 to 6.5 cm). Interestingly, the size of the large RCC in patients harboring VHL pathogenic variants (n = 9) was significantly greater than that in patients with VHL likely pathogenic (n = 7) variants (5.4 cm [3.65 to 6.6] vs. 2.9 cm [2.45 to 3.35]; p = 0.008). Moreover, adrenal paraganglioma (PGL) (82.35% vs. 17.65%; p = 0.0001) and pancreatic neuroendocrine tumor (PNET) (81.81% vs. 18.18%; p = 0.007) were associated with missense VHL pathogenic or likely pathogenic variants compared with non-missense defects. In contrast, central nervous system (CNS) hemangioblastomas (HBs) (90.47% vs. 53.12%; p = 0.004), pancreatic cysts (76.19% vs. 28.12%; p = 0.001) and RCCs (57.14% vs. 12.5; p = 0.001) were more common in patients with non-missense VHL variants.</p><p><strong>Conclusion: </strong>VHL pathogenic variants were associated with larger RCCs than were VHL likely pathogenic variants.</p>","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e240354"},"PeriodicalIF":1.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143410701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Audiological alterations in resistance to thyroid hormone syndrome: emphasizing lifelong assessment.","authors":"Alexandre Machado Silva de Oliveira, Viviane Motti, Jessica Casemiro, Pedro Resende Rende, Caio Leônidas Oliveira de Andrade, Luciene da Cruz Fernande, Helton Estrela Ramos","doi":"10.20945/2359-4292-2024-0078","DOIUrl":"10.20945/2359-4292-2024-0078","url":null,"abstract":"<p><p>The aim of this study was to investigate the long-term audiological consequences of resistance to thyroid hormone (RTH) syndrome. The cochlea and inner ear express thyroid hormone receptor beta (THRB) in developmental stages. Hearing loss is frequent in subjects with RTH syndrome; however, the long-term impact of insufficient thyroid hormone action in the auditory system remains unknown. Subjects with RTH from the same family, carrying a THRB gene variant, underwent detailed clinical evaluation and serum biochemistry analysis. The genetic assessment involved sequencing of the THRB gene. Hearing loss assessment included (i) meatoscopy, (ii) audiometric tests using pure tone audiometry, (iii) middle ear evaluation by tympanometry, (iv) transient otoacoustic emissions (TOAE), and (v) computed tomography of the mastoids. Genetic sequencing confirmed the THRB gene alteration (p.M442T) in three family members. All affected subjects had clinical and laboratory RTH features. Notably, the older subject with RTH was affected by a bilateral sensorineural hearing loss pattern affected by high frequencies, and cochlear dysfunction was also presented by TOAE analysis, indicating pronounced hearing loss. Hearing loss represents a significant concern in subjects with RTH, emphasizing the need for continuous and comprehensive audiological assessments. These findings underscore the importance of lifelong monitoring, particularly to assess age-related progression of hearing impairment.</p>","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e240078"},"PeriodicalIF":1.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does Radiofrequency Echographic Multi-Spectrometry (REMS) perform similarly to Dual-energy X-ray Absorptiometry (DXA) in terms of Trabecular Bone Score (TBS) and FRAX?","authors":"Débora Meira Ramos Amorim, Eliane Naomi Sakane, Sergio Setsuo Maeda, Marise Lazaretti-Castro","doi":"10.20945/2359-4292-2023-0456","DOIUrl":"10.20945/2359-4292-2023-0456","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate whether bone assessment by radiofrequency echographic multi-spectrometry (REMS) is influenced by trabecular bone integrity by comparing it to dual-energy X-ray absorptiometry (DXA) and the trabecular bone score (TBS). Additionally, the study aims to determine if comparing fracture risk using FRAX and the National Osteoporosis Guideline Group (NOGG) using the T-score from each method would lead to differences in a Brazilian female population.</p><p><strong>Subjects and methods: </strong>A sample of women aged 30-80 underwent REMS and DXA scans of axial sites at the Hospital São Paulo, Brazil. Subsequently, TBS was applied to DXA exams. Clinical data were obtained from hospital records and phone interviews to calculate fracture risk.</p><p><strong>Results: </strong>Among the 343 participants enrolled, 213 had comparable lumbar spine exams by REMS, DXA, and TBS, and 166 had comparable hip exams by REMS and DXA. The correlation between lumbar spine bone mineral density (BMD) by REMS and the TBS was low (r = 0.27, p < 0.001), as was the correlation between DXA and TBS (r = 0.39, p < 0.001). No statistically significant difference was found between the TBS classifications of osteoporotic lumbar spine by REMS and DXA (p = 0.178). Fracture risk data by FRAX were obtained from 119 participants, with 92% receiving concordant NOGG classifications for major osteoporotic fracture risk from REMS and DXA (κ = 0.71 CI95% (0.54 to 0.89), p < 0.001), and 87% for hip fracture risk (κ = 0.58 CI95% (0.38 to 0.77), p < 0.001).</p><p><strong>Conclusion: </strong>REMS performed similarly to DXA in assessing trabecular integrity using TBS. Additionally, no statistically significant difference was observed in fracture risk assessment by FRAX based on NOGG recommendations.</p>","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e230456"},"PeriodicalIF":1.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A paradigm shift in nutritional clinical practice: filling a gap on the implementation of the Dietary Guidelines for the Brazilian Population.","authors":"Kamila Tiemann Gabe, Carlos Augusto Monteiro","doi":"10.20945/2359-4292-2024-0446","DOIUrl":"10.20945/2359-4292-2024-0446","url":null,"abstract":"","PeriodicalId":54303,"journal":{"name":"Archives of Endocrinology Metabolism","volume":"69 1","pages":"e240446"},"PeriodicalIF":1.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}