World Journal of Mens Health最新文献

{"title":"The Role of Different Modalities of Regenerative Therapies in the Treatment of Erectile Dysfunction: A Global Survey and Global Andrology Forum Expert Recommendations.","authors":"Manaf Al Hashimi, Germar-M Pinggera, Taymour Mostafa, Rupin Shah, Bahadir Sahin, Eric Chung, Amarnath Rambhatla, Selahittin Cayan, Hiva Alipour, Maged Ragab, Omer Raheem, Mohamed Arafa, Hussain Alnajjar, Ateş Kadioglu, Abheesh Varma Hegde, Ahmed Harraz, Ashok Agarwa","doi":"10.5534/wjmh.240214","DOIUrl":"https://doi.org/10.5534/wjmh.240214","url":null,"abstract":"<p><strong>Purpose: </strong>There is increased interest in regenerative therapies (RTs) to treat erectile dysfunction (ED). However, the need for society's guidelines has led to varied practices. This study aims to investigate current global practices, address the heterogeneity in treatment protocols and evaluations, and establish expert recommendations in clinical practice.</p><p><strong>Materials and methods: </strong>Senior experts from the Global Andrology Forum (GAF) created a 32-question survey to evaluate the clinical aspects of various RT modalities and compare them with each other and with phosphodiesterase type 5 inhibitors (PDE5is). The survey was distributed worldwide to ED specialists through online Google Forms, the GAF website, international professional societies, and direct emails. The responses were analyzed and are presented as percentage frequencies. Additionally, an expert consensus on recommendations for RT use was reached at via a modified Delphi method.</p><p><strong>Results: </strong>Out of 163 respondents from 39 countries, the majority (80.1%) were using low-intensity shockwave therapy (LISWT), followed by platelet-rich plasma (PRP) 61.3% and stem cell therapy (SCT) 17.8%. Efficacy comparisons revealed no perceived significant differences among RT modalities (p=0.124). Compared to PDE5is, the efficacy of LISWT and PRP was considered lower by the respondents, while SCT was rated better by almost half of those who used SCT. The duration of improvement varied (p=0.279), with most improvements lasting 1 to 6 months. The treatment protocols used for LISWT were mainly consistent but varied widely for PRP and SCT. Adverse effects were minimal, particularly for LISWT (p<0.001). The costs varied significantly (p<0.001), with SCT being the most expensive. The evidence for efficacy was rated as primarily moderate to strong for LISWT but poor for PRP and SCT (p=0.027).</p><p><strong>Conclusions: </strong>Most respondents utilized LISWT, followed by PRP, with SCT being the least commonly utilized. The high break heterogeneity in treatment protocols and evaluation of RT underscores the need for further studies and guidelines to establish best practices.</p>","PeriodicalId":54261,"journal":{"name":"World Journal of Mens Health","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-Term Hormonal Changes Following Microdissection Testicular Sperm Extraction among Men with Non-Obstructive Azoospermia: Findings from a Large Longitudinal Prospective Multicentric Study.","authors":"Fausto Negri, Edoardo Pozzi, Christian Corsini, Massimiliano Raffo, Federico Belladelli, Alessandro Bertini, Francesco Cattafi, Eugenio Ventimiglia, Rayan Matloob, Antonino Saccà, Luca Boeri, Alessia d'Arma, Francesco Montorsi, Andrea Salonia","doi":"10.5534/wjmh.240184","DOIUrl":"https://doi.org/10.5534/wjmh.240184","url":null,"abstract":"<p><strong>Purpose: </strong>We aimed to investigate possible hormonal changes following microdissection testicular sperm extraction (mTESE) in men with non-obstructive azoospermia (NOA) across three referral centers.</p><p><strong>Materials and methods: </strong>We prospectively analyzed data from 102 consecutive NOA men. Patients with prior hormonal therapies were excluded. Preoperative serum hormone levels (total testosterone [tT], luteinizing hormone [LH], follicle-stimulating hormone [FSH], and 17β-estradiol) were collected, with repeat measurements at 3-month post-surgery. We divided the cohort into two groups using a tT cut-off value of 3 ng/mL: 1) men who kept eugonadal status; and, 2) men who were initially eugonadal but became testosterone deficient (TD) after surgery.</p><p><strong>Results: </strong>Overall, median (interquartile range [IQR]) age was 37 years (32-40 years). Positive sperm retrieval during mTESE was observed in 34 (33.3%) patients, and 48 (47.1%) underwent bilateral mTESE. Compared to baseline, 3-month postoperative median (IQR) hormonal levels were as follows: tT: 3.71 ng/mL (2.76-5.24 ng/mL) <i>vs.</i> 4.27 ng/mL (3.25-6.07 ng/mL), p=0.32; FSH: 22.0 mIU/mL (12.65-31.47 mIU/mL) <i>vs.</i> 19.5 mIU/mL (11.63-25.8 mIU/mL), p=0.25; LH: 9.0 mIU/mL (5.11-12.4 mIU/mL) <i>vs.</i> 7.6 mIU/mL (5.04-13.4 mIU/mL), p=0.73, respectively. Twelve (13.5%) eugonadal men at baseline showed TD after mTESE. Median (IQR) tT values at baseline and 3-month follow-up were compared between those who preserved eugonadal status after surgery and those who became TD after surgery: baseline levels were 4.46 ng/mL (4.1-6.27 ng/mL) <i>vs.</i> 4.14 ng/mL (3.24-4.98 ng/mL), p=0.09; and, 3-month follow-up levels were 4.58 ng/mL (3.58-5.56 ng/mL) <i>vs.</i> 2.51 ng/mL (2.31-2.76 ng/mL), p<0.001, respectively. Men who developed TD had lower testicular volume (TV) (6 [4-10] vs. 10 [8-12.25] Prader, p=0.001) and karyotype abnormalities (4 [33.3] <i>vs.</i> 1 [1.3], p=0.006).</p><p><strong>Conclusions: </strong>This multicentric study shows that mTESE in men with NOA does not significantly impact short-time postoperative follow-up tT, LH, and FSH levels. A substantial proportion of men who were initially eugonadal demonstrated tT suggestive for TD at 3-month follow-up. These men had lower TV at baseline and abnormal karyotype.</p>","PeriodicalId":54261,"journal":{"name":"World Journal of Mens Health","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coping with Complications that Occur after Prostate Biopsy for Satisfactory Evaluation of Call Service Using Artificial Intelligence: A Pilot Randomized Controlled Trial.","authors":"Yong Nam Gwon, Ukrae Cho, Seung Ryong Chong, Ji Yeon Han, Do Kyung Kim, Seung Whan Doo, Won Jae Yang, Kyeongmin Kim, Sung Ryul Shim, Jaehun Jung, Jae Heon Kim","doi":"10.5534/wjmh.240191","DOIUrl":"https://doi.org/10.5534/wjmh.240191","url":null,"abstract":"<p><strong>Purpose: </strong>To assess whether an artificial intelligence (AI)-based reassurance-call can inform patients about potential complications and provides reassurance following a prostate biopsy.</p><p><strong>Materials and methods: </strong>From October 2022 to December 2023, 42 patients aged 40 to 70 years undergoing their first prostate biopsy were recruited. The 'Reassurance-call' service was utilized to inform and monitor patients for complications. Participants were randomized into three groups: AI-assisted Reassurance-call service (Group 1), human-assisted Reassurance-call service (Group 2), and no call (Group 3). The primary outcome measured was patient satisfaction with the Reassurance-call service, assessed using a Likert scale. Secondary outcomes included satisfaction with complication management and anxiety levels, evaluated using the Likert scale, visual analog scale (VAS), and the state-trait anxiety inventory (STAI).</p><p><strong>Results: </strong>Satisfaction with Reassurance-call averaged 4.20 (standard deviation [SD] 0.56) for Group 1 and 4.71 (SD 0.47) for Group 2, showing a statistically significant difference. Satisfaction regarding complication management using Likert scale was 4.13 (SD 0.52) for Group 1, 4.43 (SD 0.76) for Group 2, and 3.79 (SD 0.80) for Group 3 with no statistically significant differences. Satisfaction regarding complication management using VAS averaged 8.33 (SD 1.23) for Group 1, 8.57 (SD 1.45) for Group 2, and 7.07 (SD 1.86) for Group 3, indicating significant differences. Anxiety levels using STAI averaged 40.00 (SD 10.54) for Group 1, 39.14 (SD 8.29) for Group 2, and 35.00 (SD 9.46) for Group 3, showing no significant differences. Anxiety levels using VAS averaged 5.07 (SD 2.79) for Group 1, 2.21 (SD 2.64) for Group 2, and 3.50 (SD 2.28) for Group 3, with significant differences observed.</p><p><strong>Conclusions: </strong>AI demonstrated potential in enhancing patient reassurance and managing complications post-prostate biopsy, although human interaction proved superior in certain aspects. Further studies with larger cohorts are necessary to verify the effectiveness of AI-based tools.</p>","PeriodicalId":54261,"journal":{"name":"World Journal of Mens Health","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Dietary Habits on Male Lower Urinary Tract Symptoms: Differential Influence of Water Intake on Voiding Symptoms and Storage Symptoms.","authors":"Woo Suk Choi, Nam Ju Heo, Hwancheol Son","doi":"10.5534/wjmh.230361","DOIUrl":"https://doi.org/10.5534/wjmh.230361","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the dietary factors affecting male lower urinary tract symptoms (LUTS).</p><p><strong>Materials and methods: </strong>This retrospective study analyzed men who underwent health check-ups. The men who completed the International Prostate Symptom Score (IPSS) and a dietary questionnaire with 19 items were included in the study. Men with a history of medication for LUTS were excluded from the study. The influences of each dietary habit on total IPSS, voiding symptoms, storage symptoms and quality of life were evaluated separately. Dietary risk group was defined by the number of significant dietary risk factors.</p><p><strong>Results: </strong>The mean age of 28,463 men was 52.2±8.7 years. After adjusting for age and other conditions, 13 dietary habits (for examples; overeating, preference for salty food, frequently eating sweets, and infrequently eating vegetables, etc.) were the significant risk factors related to worse total IPSS scores. For voiding symptoms, storage symptoms, and quality of life scores, 14, 12, and 12 dietary habits were identified as independent risk factors, respectively. Drinking four or more cups of water per day was related to worse storage symptoms (odds ratio [OR]=1.12, 95% confidence interval [95% CI]=1.09-1.22), but better voiding symptoms (OR=0.90, 95% CI=0.86-0.95). The newly developed dietary risk group showed that total and subtotal IPSS scores increased by the number of bad dietary habits in all age groups, respectively.</p><p><strong>Conclusions: </strong>This study showed that dietary habits had a significant impact on LUTS. The amount of water consumed had a differential influence on each subdomain symptom.</p>","PeriodicalId":54261,"journal":{"name":"World Journal of Mens Health","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the Potential of ChatGPT in Male Sexual Health and Dysfunction: A Hold Endeavor and Comprehensive Study.","authors":"Biao Ran, Puze Wang, Liangren Liu","doi":"10.5534/wjmh.240228","DOIUrl":"https://doi.org/10.5534/wjmh.240228","url":null,"abstract":"","PeriodicalId":54261,"journal":{"name":"World Journal of Mens Health","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postoperative Progress of Deep Grid Incision and Sealing with Collagen Fleece for Treatment of Peyronie's Disease: Prospective Observational Study for 3 Years.","authors":"Du Geon Moon, Sun Beom Cho, Won Ku Hwang, Hyo Jong Kim, Sun Tae Ahn","doi":"10.5534/wjmh.240201","DOIUrl":"https://doi.org/10.5534/wjmh.240201","url":null,"abstract":"<p><strong>Purpose: </strong>Despite recent popularity, partial plaque excision and sealing with TachoSil has concern about tunica regeneration from graft and lack of long-term results. Previously, we introduced multiple deep grid incisions of Peyronie's plaque to minimize tunical defect with consequent veno-occlusal erectile dysfunction. To assess the efficacy of modified grid incision of plaque and sealing with collagen fleece in postoperative progress of 34 patients for 3 years.</p><p><strong>Materials and methods: </strong>From Aug 2018, 34 patients with stable Peyronie's disease (PD) underwent surgery involving three major steps: 1) dissection of the neurovascular bundle or urethra according to plaque location, 2) multiple deep grid incisions of plaque for complete curvature correction, and 3) sealing with collagen fleece without suture. We assessed the stretched penile length, totally straightness, penile sonography, erectile function preoperatively and 3, 6, 12 months and annually postoperatively. This study was approved by the Institutional Review Board.</p><p><strong>Results: </strong>Mean age was 59.4 years (29-72 years). Mean curvature was 53.5 degree (35-100 degree), with hinge and hourglass deformity in 12 and 8 patients, respectively. Five patients required inflatable penile prosthesis (IPP) insertion, with one more at 30 months. The mean follow-up was 42.3 months. Penile rehabilitation, including daily massage, reduced subcutaneous thickening by 12 months postoperatively. All patients initially achieved complete straightness, with two experiencing recurrent curvature. Four patients had subcutaneous hematomas, subsiding in two. Minor skin issues occurred in three IPP patients. Postoperative erectile function was satisfactory in 85.0% of patients. Most regained preoperative length by 1.6 years. Global Assessment Questionniare satisfaction increased from 69.0% at 1 year to 90.0% until 3 years.</p><p><strong>Conclusions: </strong>The modified grid incision with collagen fleece sealing effectively treats PD without causing tunica albuginea defects. Long-term follow-up is essential for monitoring erectile function and penile length recovery, ensuring successful clinical outcomes.</p>","PeriodicalId":54261,"journal":{"name":"World Journal of Mens Health","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Correlation Between Frailty and Lower Urinary Tract Symptoms in Elderly Male Using Korean-FRAIL Scale.","authors":"Zhao Luo, Chao Niu, ChenYu Yuan, Dong Yun Lee, Yu Seob Shin, Sung Chul Kam","doi":"10.5534/wjmh.240122","DOIUrl":"https://doi.org/10.5534/wjmh.240122","url":null,"abstract":"<p><strong>Purpose: </strong>The K-FRAIL scale is a Korean version of the aging questionnaire that allows screening and diagnosis of easily observable aging in the elderly through a simple self-answer questionnaire. The objective of this study was to examine the relation between fraility and clinical symptoms of the lower urinary tract in elderly men using the K-FRAIL scale.</p><p><strong>Materials and methods: </strong>A prospective study was conducted on 100 patients who underwent urological examination at our hospital from January 2021 to December 2021. Among them, 62 patients who met the study criteria completed the following questionnaires and lower urinary tract symptoms (LUTS) parameters: K-FRAIL, International Prostate Symptom Score (IPSS), prostate specific antigen, transrectal ultrasonography, and uroflowmetry. In this cross-sectional study, we compared the K-FRAIL scale parameters with various clinical examination parameters in elderly men presenting with LUTS. We also assessed the time required to complete the scale.</p><p><strong>Results: </strong>The age of the subjects was 66.8±5.96 years in the normal group and 71.6±9.83 years in the frail group (p=0.027). In terms of IPSS indicators, the total scores of the natural patient group and the frail patient group were 13.24±5.48 and 20.44±9.67 (p=0.001); in terms of patient serum testosterone indicators, the scores of the natural patient group and the frail patient group were 4.32±1.52 and 3.74±1.64, respectively (p=0.163). The results indicated that IPSS item 7 and serum testosterone were significant predictors (odds ratio [OR]=4.679, p=0.29; OR=0.391, p=0.043, respectively).</p><p><strong>Conclusions: </strong>The results of this study show that elderly men with fraility are more likely to develop nocturia and lower testosterone. When treating men with fraility, it will be helpful to know these characteristics and treat them.</p>","PeriodicalId":54261,"journal":{"name":"World Journal of Mens Health","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yes-Associated Protein Promotes Endothelial-Mesenchymal Transition to Mediate Diabetes Mellitus Erectile Dysfunction by Phosphorylating Smad3.","authors":"Ming Xiao, Xiaoli Tan, Huanqin Zeng, Biao Liu, Xiaopeng Tang, Yanghua Xu, Yinghao Yin, Jiarong Xu, Zhitao Han, Zitaiyu Li, Yuxin Tang, Liangyu Zhao","doi":"10.5534/wjmh.240126","DOIUrl":"https://doi.org/10.5534/wjmh.240126","url":null,"abstract":"<p><strong>Purpose: </strong>The main objective of this study is to elucidate the role of endothelial-mesenchymal transition (EndMT) regulated by yes-associated protein (YAP) on diabetes mellitus erectile dysfunction (DMED).</p><p><strong>Materials and methods: </strong>High concentrations of glucose and palmitic acid (HGP) culturing simulated a diabetic condition <i>in vitro</i>. Cell proliferation, migration, tube formation, and marker gene changes of rat cavernous endothelial cells (RCECs) were measured after YAP overexpression or knockdown. Erectile function and histological outcomes were evaluated <i>in vivo</i>.</p><p><strong>Results: </strong>Nuclear YAP in RCECs was significantly increased after pretreatment with HGP. YAP overexpression enhanced the cell proliferation (0.236±0.004 <i>vs.</i> 0.148±0.008, p<0.001), migration (1.908±0.099 <i>vs.</i> 1.000±0.116, p<0.001), and tube formation (290.6±10.96 and 21,440.3±762.9 <i>vs.</i> 175.0±24.82 and 13,538.6±1,819.0, p<0.001) compared to the control group. Moreover, the ratios of intracavernous pressure (ICP) to mean arterial pressure (MAP) (0.642±0.051 <i>vs.</i> 0.850±0.070, p<0.05), and smooth muscle to collagen (0.155±0.010 <i>vs.</i> 0.274±0.023, p<0.01) were decreased in rats with YAP overexpression. The effects of HGP on CD31, eNOS, CD34, VE-cadherin, vimentin, α-SMA, and p-Smad3 expression were abrogated by inhibiting YAP in RCECs. YAP knockdown also restored the ICP/MAP (0.597±0.019 <i>vs.</i> 0.346±0.033, p<0.01), smooth muscle/collagen (0.13±0.010 <i>vs.</i> 0.08±0.026, p<0.05) and p-Smad3/Smad3 (1.61±0.291 <i>vs.</i> 3.26±0.332, p<0.01) ratios in type 2 diabetic rats.</p><p><strong>Conclusions: </strong>YAP promotes EndMT to impair erectile function in type 2 diabetic rats by phosphorylating Smad3, providing a new strategy for treating DMED.</p>","PeriodicalId":54261,"journal":{"name":"World Journal of Mens Health","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Combination of <i>Curcumae Radix</i> and Syzygium Aromaticum Extracts Mitigates Benign Prostatic Hyperplasia through Anti-Proliferative and Anti-Inflammatory Effects.","authors":"Geum Hwa Lee, Yu Seob Shin, Ji Hyun Kim, Da Hee Shim, Hwa Young Lee, Luo Zhao, Mohammad Mamun Ur Rashid, Hwang Eui Cho, Jong Jin Lee, Min Kyoung Kim, Do Sung Kim, Hyun Jun Park, Han Jung Chae","doi":"10.5534/wjmh.240071","DOIUrl":"https://doi.org/10.5534/wjmh.240071","url":null,"abstract":"<p><strong>Purpose: </strong>Benign prostatic hyperplasia (BPH) is a urinary tract disorder that primarily affects geriatric males. Natural materials have been developed to treat and prevent symptoms of BPH. However, a few natural functional foods has been conclusively proven to cure or prevent symptoms of BPH. The study aimed to investigate the anti-proliferative mechanism of <i>Curcumae Radix</i> (CR) and Syzygium aromaticum (SA) extracts using RWPE-1 cells and testosterone propionate (TP)-induced BPH rats.</p><p><strong>Materials and methods: </strong><i>In vitro</i> experiments were performed to assess the synergistic anti-proliferative effects of an equal mixture of CR and SA extracts on TP-treated RWPE-1 cells. In animal experiments, TP-induced BPH rats were administrated with saline, CR and SA extracts at 50, 100, and 200 mg/kg or finasteride at 1 mg/kg daily for 6 weeks. Body weight, prostate weight, dihydrotestosterone (DHT), androgen receptor (AR), and prostate-specific antigen (PSA) levels were measured. Additionally, extracellular signal-regulated kinase and NF-κB levels, oxidative stress, and inflammatory stress responses in the prostate were also analyzed.</p><p><strong>Results: </strong>In this study, the combination of CR and SA extracts synergistically inhibited cell proliferation compared with the effect of each extract in TP-treated RWPE-1 cells. CR and SA extracts inhibited increasing of prostate weight, thickness of prostate epithelium, the level of PSA and DHT in serum. The expression of protein and gene of PSA and AR in prostate of TP-induced BPH rats were also suppressed by the administration of CR and SA extracts. Furthermore, reactive oxygen species and inflammation axis, NOX4-iNOS-COX2 and its associated representative inflammatory cytokine, interleukin-8 were also reduced in the CR and SA extracts-administered BPH rats.</p><p><strong>Conclusions: </strong>The results suggest that the combination of CR and SA extracts improves BPH through its anti-inflammatory and anti-oxidative effects, demonstrating great potential as an anti-BPH medicine.</p>","PeriodicalId":54261,"journal":{"name":"World Journal of Mens Health","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142548932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial FIS1 As a Novel Drug Target for the Treatment of Erectile Dysfunction: A Multi-Omic and Epigenomic Association Study.","authors":"Tianle Zhu, Pan Gao, Yukuai Ma, Peng Yang, Zhi Cao, Jingjing Gao, Junhua Du, Hui Jiang, Xiansheng Zhang","doi":"10.5534/wjmh.240131","DOIUrl":"https://doi.org/10.5534/wjmh.240131","url":null,"abstract":"<p><strong>Purpose: </strong>Mitochondrial dysfunction may impact male erectile function, but the underlying genetic mechanisms remain unclear. The study aims to investigate the causal role for genetically regulated mitochondrial function in erectile dysfunction (ED) and to identify potential drug targets for the treatment of ED.</p><p><strong>Materials and methods: </strong>The data of gene methylation, gene expression and protein level related to mitochondria were extracted from the corresponding genome-wide association studies (GWAS) database. Discovery and validation datasets for ED were sourced from research by Bovijn et al, the FinnGen database, and the UK Biobank. We performed summary-data-based Mendelian randomization analysis, colocalization analysis, as well as molecular prediction and molecular docking analysis to assess the association between mitochondrial dysfunction and ED. We also identified relevant targets and potential molecules for the treatment of ED.</p><p><strong>Results: </strong>Through the integration of multi-omics results, we identified an inverse relationship between the expression of the mitochondrial-related gene <i>FIS1</i> and the risk of ED (odds ratio [OR]: 0.89, 95% confidence interval [CI]: 0.81-0.97, p-value of summary-data-based Mendelian randomization analysis [PSMR]=1.01×10^-2). In FIS1, methylation of cg19802458 and cg08601038 was related to high expression of the <i>FIS1</i> gene, which is consistent with the protective effects of cg19802458 and cg08601038 methylation against ED. Additionally, elevated levels of FIS1 protein displayed a negative association with ED risk (OR: 0.71, 95% CI: 0.55-0.92, PSMR=1.00×10^-2). Molecular prediction and molecular docking analysis revealed that resveratrol and quercetin had protective effects against ED by targeting FIS1, and had good affinity and binding mode with FIS1, respectively.</p><p><strong>Conclusions: </strong>This study demonstrated the causal relationship between the mitochondrial <i>FIS1</i> gene and ED risk and found that resveratrol and quercetin may have therapeutic effects on ED. These discoveries offer fresh perspectives on the pathogenesis of ED and propose preliminary candidate targets and drugs for future treatment of ED.</p>","PeriodicalId":54261,"journal":{"name":"World Journal of Mens Health","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142548931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}