SLAS Technology最新文献

{"title":"Clinical characteristics and risk factors of premature rupture of membranes infection in pregnant and lying-in women","authors":"Shufang Xiao,&nbsp;Meimei Lin","doi":"10.1016/j.slast.2025.100320","DOIUrl":"10.1016/j.slast.2025.100320","url":null,"abstract":"<div><div>Premature rupture of membranes is one of the more common symptoms of pregnant women before labor, which can lead to an increased rate of preterm birth and a higher mortality rate of the fetus born from it. The current research on premature rupture of membranes (PROM) is mainly based on multivariate regression analysis, and variables are selected for multivariate regression analysis after univariate analysis. This method may omit some independent variables, resulting in one-sided analysis results. In this context, this study uses Bayesian method and Logistic regression analysis to construct a new variable analysis model to analyze the clinical characteristics and risk factors of PROM infection. First, through Bayesian Logistic regression, the clinical features of PROM infection mainly include fever, increased white blood cells and C-reactive protein, and increased fetal heart rate. The analysis of risk factors showed that pathogen infection, maternal pregnancy number, and scarred uterus were all risk factors for PROM infection. Finally, in order to explain the effect of the analysis model used in this paper, a nonparametric test, AUC value and ROC curve were used to compare the effect of Bayesian Logistic regression and Logistic regression. The results showed that the statistic value of Bayesian logistic regression was 0.177 higher than that of logistic regression, and the AUC value was 0.014 higher. That is, the performance of the Bayesian logistic regression model is better. The method used in the experiment is feasible, and the experimental results are in line with expectations.</div></div>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":"33 ","pages":"Article 100320"},"PeriodicalIF":2.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomal miRNA-188–3p derived from cancer-associated fibroblasts promotes ferroptosis in cervical cancer: Medical biothermal image analysis","authors":"Xiao Li,&nbsp;Min Han","doi":"10.1016/j.slast.2025.100313","DOIUrl":"10.1016/j.slast.2025.100313","url":null,"abstract":"<div><div>The study aimed to explore the potential mechanism of action of extracellular miRNA-188–3p derived from CAFs in cervical cancer. In this study, CAFs were isolated from patients with cervical cancer, and exosomes were extracted by ultrafast centrifugation method to detect the expression level of miRNA-188–3p in exosomes. Subsequently, the exosomes were co-cultured with cervical cancer cells, and the temperature changes of the cells were monitored by medical thermal image analysis technology to evaluate the metabolic activity of the cells. Western blot and qPCR were used to detect protein and mRNA expression levels related to iron metabolism in order to investigate the role of miRNA-188–3p in iron metabolism of cervical cancer cells. The results showed that the expression level of miRNA-188–3p in exosomes derived from CAFs was significantly higher than that of exosomes derived from normal fibroblasts. Medical thermal image analysis showed that cervical cancer cells treated with miRNA-188–3p showed higher metabolic activity, manifested by increased temperature. The results of cell proliferation test, scratch test and Transwell invasion test all showed that miRNA-188–3p promoted the proliferation, migration and invasion of cervical cancer cells. Further molecular mechanism studies showed that miRNA-188–3p regulates iron homeostasis in cervical cancer cells by targeting genes related to iron metabolism, thereby promoting cell proliferation and invasion.</div></div>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":"33 ","pages":"Article 100313"},"PeriodicalIF":2.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing anterior and posterior circulation strokes using high-resolution magnetic resonance imaging","authors":"Hongshan Chu ,&nbsp;Shibo Dong ,&nbsp;Yuan Zhang ,&nbsp;Hongyu Hao ,&nbsp;Ruisheng Duan ,&nbsp;Xing Xing ,&nbsp;Nan Yin ,&nbsp;Jin An ,&nbsp;Ya Gao ,&nbsp;Xiangjian Xiao","doi":"10.1016/j.slast.2025.100319","DOIUrl":"10.1016/j.slast.2025.100319","url":null,"abstract":"<div><h3>Background and Purpose</h3><div>High-resolution magnetic resonance imaging (HR-MRI) is increasingly used to assess intracranial atherosclerosis (ICAS). The risks for anterior circulation stroke (ACS) and posterior circulation stroke (PCS) differ, and understanding the factors influencing these differences is important for better clinical management. This study investigates the impact of clinical risk factors on ICAS features in ACS and PCS using HR-MRI, focusing on arterial remodeling patterns.</div></div><div><h3>Methods</h3><div>This retrospective, cross-sectional study uses HR-MRI to investigate the differences in plaque characteristics and remodeling patterns between anterior and posterior circulation strokes (ACS and PCS). Consecutive patients with acute ischemic stroke or transient ischemic attack (TIA) who received HR-MRI from January 2021 to August 2022 were enrolled. We compared ACS and PCS cases, analyzed clinical and radiological features, and examined factors associated with arterial remodeling patterns.</div></div><div><h3>Results</h3><div>Of 208 patients, 123 had ACS, and 85 had PCS. PCS was significantly associated with diabetes (<em>P</em> = 0.036) and positive remodeling (<em>P</em> = 0.01). Differences in HbA1c, fibrinogen levels, and plaque enhancement were observed between ACS and PCS (<em>P</em> &lt; 0.001; <em>P</em> = 0.033; <em>P</em> = 0.03). Multivariable analysis identified smoking (<em>P</em> = 0.048; OR: 1.96, 95 % CI: 1.01–3.81) and uric acid levels (<em>P</em> = 0.022; OR: 0.996, 95 % CI: 0.993–0.999) as independent factors associated with non-positive remodeling.</div></div><div><h3>Conclusions</h3><div>HR-MRI reveals significant differences in risk factors and plaque characteristics between ACS and PCS, with smoking and uric acid levels associated with non-positive remodeling. These findings highlight the importance of HR-MRI in identifying plaque vulnerability and guiding clinical interventions.</div></div>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":"33 ","pages":"Article 100319"},"PeriodicalIF":2.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical observation and evaluation of health management intervention in controlling senile chronic diseases such as hyperlipidemia","authors":"Hongxia Liu","doi":"10.1016/j.slast.2025.100318","DOIUrl":"10.1016/j.slast.2025.100318","url":null,"abstract":"<div><div>Irregular diet, lack of exercise, and poor sleep quality have led to various diseases, with chronic conditions like hyperlipidemia being most common among the elderly. With advances in the medical Internet of Things (MIoT), medical data now supports more effective care strategies. Health management intervention, as a novel approach, aims to improve elderly patients’ understanding and management of chronic diseases. In this study, 200 elderly patients with hyperlipidemia were randomly divided into a control group (routine care) and an experimental group (health management intervention). Results showed that health knowledge mastery improved significantly with intervention—rising from 61.8 % to 74.4 % in males and from 56.8 % to 71.6 % in females. Thus, health management intervention based on medical data effectively enhances patients’ health literacy.</div></div>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":"33 ","pages":"Article 100318"},"PeriodicalIF":2.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sample preparation using multiple microbial pattern recognition proteins and magnetic bead ratcheting","authors":"Jessica Martinez ,&nbsp;Dong Jin M. Park ,&nbsp;Samantha Abate ,&nbsp;James Hill ,&nbsp;George Downey ,&nbsp;Craig Galligan ,&nbsp;Tyler Hammond ,&nbsp;Michael T. McCurdy ,&nbsp;Surekha Gurung ,&nbsp;Shanjana Shawon ,&nbsp;Ralf Lenigk ,&nbsp;Kuangwen Hsieh ,&nbsp;Michael Super ,&nbsp;Erik Kvam ,&nbsp;Tza-Huei Wang ,&nbsp;Coleman Murray ,&nbsp;Chris Puleo","doi":"10.1016/j.slast.2025.100315","DOIUrl":"10.1016/j.slast.2025.100315","url":null,"abstract":"<div><div>Sample preparation (i.e., isolation and purification of pathogens from samples) remains one of the bottlenecks limiting deployment of diagnostic tests. Two recent examples highlighting these limitations include the difficulties in widespread nucleic acid testing during COVID-19 and in identifying drug-resistant infections. Unmet needs include systems that work with different bodily fluids and samples, multiple types of suspected pathogen, and function in a rapid and semi-automated fashion. Advances such as these could accelerate the deployment of novel diagnostic tests by eliminating upstream sample preparation bottlenecks.</div><div>Herein, we tested the feasibility of combining multiple pathogen-binding paramagnetic beads with magnetic ratcheting-based enrichment to directly isolate microbes from samples. We demonstrate effective use of three different paramagnetic bead-conjugated proteins (mannose binding lection [MBL], C-reactive protein [CRP], and dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin [DC-SIGN]) to capture 18 different bacteria species and model virus particles/peptides. We used magnetic ratcheting to isolate bead-bound microbes from milliliters (mLs) of sample at concentrations of 4 – 4000 microbes per mL. The flow-through system was operated up to 1 mL per minute and enabled pathogen isolation from 10 mL samples in &lt;30 min. Demonstration of post-ratcheting PCR-based microbe analysis was also performed, showing that the technology may have applicability across different infectious agents, sample types/volumes, and analytical assays. Based on these results, further studies are warranted to test clinical samples, compare results to current gold-standard diagnostic methods, and test the sample preparation technologies across additional pathogen types.</div></div>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":"33 ","pages":"Article 100315"},"PeriodicalIF":2.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of PTEN, Akt and CREB genes in trigeminal ganglia of intractable migraine","authors":"Zheyuan Song ,&nbsp;Luyao Zhou ,&nbsp;Jiasi Li","doi":"10.1016/j.slast.2025.100312","DOIUrl":"10.1016/j.slast.2025.100312","url":null,"abstract":"<div><div>Migraine is a kind of common clinical headache. Because its etiology is unknown and the curative effect is not very ideal, recent studies have shown that the activation of trigeminal ganglion has a certain impact on the occurrence of migraine. In this paper, the rat migraine model was established by subcutaneous injection of nitroglycerin. Reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical techniques were used to observe the changes and correlation of the phosphatase and tensin homolog deleted on chromosome ten (PTEN), protein kinase B (Akt) and cAMP response element binding protein (CREB) expressions in trigeminal ganglia of migraine rats at gene and protein levels, respectively, in order to explore the relationship and role of these three genes in the pathogenesis of migraine. It can be seen from the experimental results that after the treatment of nickel carbohydrazide perchlorate (GTN), the expression level of PTEN mRNA in trigeminal ganglion decreased significantly, and it was the lowest at 12 h of GTN, with a value of 0.21. However, over time, PTEN mRNA expression level has rose to 0.44 after GTN24h, reaching the initial level. According to the calculation, PTEN was significantly negatively correlated with Akt protein expression, and its P value was &lt;0.05. The experiment in this paper first proved that the attack rate of migraine in women was higher than that in men, and then proved the relationship between PTEN, Akt and CREB genes in the trigeminal ganglion of migraine.</div></div>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":"33 ","pages":"Article 100312"},"PeriodicalIF":2.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acoustic ejection mass spectrometry: An integrated pipeline for ultra-high throughput screening, reactivity profiling, and potency analysis of covalent BTK inhibitors","authors":"Ronghai Cheng ,&nbsp;Adil Muneer ,&nbsp;Maria Hercher ,&nbsp;Bekim Bajrami ,&nbsp;Reza Nemati","doi":"10.1016/j.slast.2025.100307","DOIUrl":"10.1016/j.slast.2025.100307","url":null,"abstract":"<div><div>Covalent drug discovery has garnered renewed interest due to its potential to target proteins previously considered \"undruggable.\" Intact protein mass spectrometry (MS) is a critical technique for providing direct evidence of covalent drug modifications to protein targets. However, its application for screening covalent libraries has been hindered by low assay throughput, complex sample preparation, and high protein consumption associated with traditional liquid chromatography-MS (LC-MS) or solid-phase extraction-MS (SPE-MS) platforms. The recent integration of acoustic ejection (AE) with electrospray ionization (ESI) source of high-resolution time-of-flight (TOF) mass spectrometers—specifically, the SCIEX Echo® MS+ with the ZenoTOF 7600—has enabled the direct introduction of intact proteins without desalting at nanoliter volumes from 384 or 1536 well plates into the electrospray ionization (ESI) source of the mass spectrometer, achieving analysis rates of 1–2 seconds per sample. This advancement offers significant potential for covalent library screening and kinetic studies of identified hits due to ultrafast sample introduction and minimal sample consumption. To fully automate this pipeline, the SCIEX Echo® MS+ with ZenoTOF 7600 mass spectrometer was integrated with our internal automation system (HighRes Biosolutions) and the data analysis workflow was automated. Using Bruton’s tyrosine kinase (BTK) as a model, we demonstrated that this integrated pipeline could accelerate covalent drug discovery through covalent library screens, off-target reactivity assessment via GSH reactivity assays, and potency evaluation through k_inact/K_i measurements.</div></div>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":"33 ","pages":"Article 100307"},"PeriodicalIF":2.5,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144254755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput mass spectrometry in drug discovery","authors":"Chang Liu","doi":"10.1016/j.slast.2025.100292","DOIUrl":"10.1016/j.slast.2025.100292","url":null,"abstract":"","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":"32 ","pages":"Article 100292"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Literature highlights column: From the literature life sciences discovery and technology highlights","authors":"Tal Murthy ,&nbsp;Jamien Lim","doi":"10.1016/j.slast.2025.100280","DOIUrl":"10.1016/j.slast.2025.100280","url":null,"abstract":"","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":"32 ","pages":"Article 100280"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CircTNFRSF19 facilitates triple negative breast cancer cell growth by regulating N6-methyladenosine modification of B3GNT5: Medical biological image simulation","authors":"Lei Xue ,&nbsp;Yuhui Zhou ,&nbsp;Feng Liu ,&nbsp;Jie Dang ,&nbsp;Yu Yan","doi":"10.1016/j.slast.2025.100308","DOIUrl":"10.1016/j.slast.2025.100308","url":null,"abstract":"<div><div>The role of cell surface receptors and glycosylation modification in cancer development has become a focus of research. In particular, the role of tumor necrosis factor receptor superfamily member 19 (TNFRSF19) and β-1, 3-N-acetylglucosamine transferase 5 (B3GNT5) in tumor cell growth has attracted extensive attention. The aim of this study was to investigate the role of CircTNFRSF19 in regulating the N6-methyladenosine (m6A) modification of B3GNT5 and how this modification promotes the growth of TNBC cells. The expression levels of CircTNFRSF19 and B3GNT5 in TNBC cell lines were detected by real-time quantitative PCR (qPCR). Then the m6A modification pattern of B3GNT5 was analyzed by m6A methylation sequencing technology, and the interaction between CircTNFRSF19 and B3GNT5 was verified by RNA immunoprecipitation (RIP) experiment. Through medical thermal image simulation technology, we conducted real-time monitoring and analysis of temperature changes during cell growth to assess the effects of CircTNFRSF19 and B3GNT5m6A modifications on cell metabolism and growth rate. The RIP experiment further confirmed the direct interaction between CircTNFRSF19 and B3GNT5. CRISPR/Cas9 gene editing experiments showed that after CircTNFRSF19 was knocked out, the m6A modification level of B3GNT5 was significantly decreased, and the growth rate of TNBC cells was also significantly slowed down. The application of medical thermal image simulation technology revealed that the metabolic activity of the cells in the CircTNFRSF19 knockout group was reduced, and the temperature change of the cell growth area was significantly different from that in the control group.</div></div>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":"33 ","pages":"Article 100308"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144212302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}