SLAS Technology最新文献

Management of experimental workflows in robotic cultivation platforms. 机器人培养平台的实验工作流程管理。

IF 2.5 4区医学

SLAS Technology Pub Date : 2024-10-30 DOI: 10.1016/j.slast.2024.100214

Lucas Kaspersetz, Britta Englert, Fabian Krah, Ernesto C Martinez, Peter Neubauer, M Nicolas Cruz Bournazou

引用次数: 0

Application of conjugated polymer nanocomposite materials as biosensors in rehabilitation of ankle joint injuries in martial arts sports. 共轭聚合物纳米复合材料作为生物传感器在武术运动踝关节损伤康复中的应用。

IF 2.5 4区医学

SLAS Technology Pub Date : 2024-10-28 DOI: 10.1016/j.slast.2024.100213

Yuan Zheng, Xiaoxiao Ren, Linzhen Li

{"title":"Application of conjugated polymer nanocomposite materials as biosensors in rehabilitation of ankle joint injuries in martial arts sports.","authors":"Yuan Zheng, Xiaoxiao Ren, Linzhen Li","doi":"10.1016/j.slast.2024.100213","DOIUrl":"https://doi.org/10.1016/j.slast.2024.100213","url":null,"abstract":"<p><p>In order to understand the application of conjugated polymer nanocomposites as biosensors in the rehabilitation of ankle joint injuries in martial arts, the author proposes a study on the application of conjugated polymer nanocomposites in the rehabilitation of ankle joint injuries in martial arts. Firstly, in martial arts training, the incidence of ankle joint injuries is relatively high. In order to prevent and reduce ankle joint injuries, high-intensity martial arts training should be used to evaluate the degree of ankle joint injuries in a timely manner using an ankle joint injury assessment model. Secondly, a Firefly algorithm based modeling method for the evaluation of ankle injury in high-intensity martial arts training is proposed. Finally, 180 questionnaires were distributed and 150 were collected. Three incomplete questions were removed, resulting in 130 valid questions with a yield of 90. The firefly algorithm has been used to assess ankle injuries and to characterize different types of combat shooting in high-intensity exercise competitions. received ankle injury index assessment combat performance. A chaotic sequence is used to fire and established a standard measurement of effort for combat ankle injuries. The proposed solution has been scientifically proven to improve basketball performance levels.</p>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142548923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of m6A-related lncRNAs prognostic signature for predicting immunotherapy response in cervical cancer 鉴定用于预测宫颈癌免疫疗法反应的 m6A 相关 lncRNAs 预后特征

IF 2.5 4区医学

SLAS Technology Pub Date : 2024-10-28 DOI: 10.1016/j.slast.2024.100210

{"title":"Identification of m6A-related lncRNAs prognostic signature for predicting immunotherapy response in cervical cancer","authors":"","doi":"10.1016/j.slast.2024.100210","DOIUrl":"10.1016/j.slast.2024.100210","url":null,"abstract":"<div><h3>Background</h3><div><em>N</em>6-methylandenosine-related long non-coding RNAs (m<sup>6</sup>A-related lncRNAs) play a crucial role in the cancer progression and immunotherapeutic efficacy. The potential function of m<sup>6</sup>A-related lncRNAs signature in cervical cancer has not been systematically clarified.</div></div><div><h3>Methods</h3><div>RNA-seq and the clinical data of cervical cancer were extracted from The Cancer Genome Atlas. All of the patients were randomly classified into training and testing cohorts. The m<sup>6</sup>A-related lncRNAs prognostic model was constructed by LASSO regression using data in the training cohort.The predictive value of the signature was validated in the whole cohort and testing cohort. Cervical cancer patients were divided into low- and high-risk subgroups by the median value of risk scores. Kaplan-Meier analysis, principal-component analysis (PCA), functional enrichment annotation, and nomogram were used for further evaluation. We also examined the immune response and potential drug sensitivity targeting this model.</div></div><div><h3>Results</h3><div>Seventy-nine prognostic m<sup>6</sup>A-related lncRNAs were screened. The risk model comprising four m<sup>6</sup>A-related lncRNAs (AL139035.1, AC015922.2, AC073529.1, AC008124.1) was identified and verified as an independent prognostic predictor of cervical cancer. A nomogram based on age, tumor grade, clinical stage, TNM stage, and four m<sup>6</sup>A-related lncRNAs risk signatures was generated. It displayed good accuracy and reliability in predicting the overall survival of patients with CC. Based on our risk model, cervical cancer patients with potential immunotherapy benefits from the candidate drugs could be effectively screened.</div></div><div><h3>Conclusion</h3><div>The four m<sup>6</sup>A-related lncRNAs signature may provide new targets and allow the prediction of immunotherapy response, which can assist developing individualized treatment for cervical cancer.</div></div>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142552829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regional developers’ community accelerates laboratory automation 地区开发者社区加速实验室自动化。

IF 2.5 4区医学

SLAS Technology Pub Date : 2024-10-24 DOI: 10.1016/j.slast.2024.100211

引用次数: 0

Deciphering the role of heat shock protein HSPA1L: biomarker discovery and prognostic insights in Parkinson's disease and glioma 解读热休克蛋白 HSPA1L 的作用：发现帕金森病和胶质瘤的生物标志物并深入了解其预后。

IF 2.5 4区医学

SLAS Technology Pub Date : 2024-10-23 DOI: 10.1016/j.slast.2024.100212

{"title":"Deciphering the role of heat shock protein HSPA1L: biomarker discovery and prognostic insights in Parkinson's disease and glioma","authors":"","doi":"10.1016/j.slast.2024.100212","DOIUrl":"10.1016/j.slast.2024.100212","url":null,"abstract":"<div><h3>Background</h3><div>Heat shock proteins (HSPs) play a critical role in cellular stress responses and have been implicated in numerous diseases, including Parkinson's disease (PD) and various cancers. Understanding the differential expression and functional implications of HSPs in these conditions is crucial for identifying potential therapeutic targets and biomarkers for diagnosis and prognosis.</div></div><div><h3>Methods</h3><div>We utilized combined datasets (GSE6613 and GSE72267) to identify and analyze the heat shock-related genes differentially expressed in PD. Gene Set Variation Analysis (GSVA) was performed to explore functional profiles, while LASSO regression was employed to screen potential PD biomarkers. In glioma, prognostic value, immune infiltration, and pathway enrichment associated with HSPA1L gene expression were assessed via Kaplan-Meier plots, ssGSEA, and enrichment analyses.</div></div><div><h3>Results</h3><div>In PD, we identified 17 differentially expressed HSPs. Enrichment analysis revealed significant pathways related to protein homeostasis and cellular stress responses. LASSO regression pinpointed 12 genes, including HSPA1L, as significant markers for PD, with nomogram and calibration plots indicating predictive accuracy. Stratification based on HSPA1L expression in PD highlighted differentially active biological processes, immune responses, and metabolic disruptions. In the pan-cancer analysis, HSPA1L showed variable expression across cancer types and a significant correlation with patient survival and immune infiltration. In glioma, low HSPA1L expression was associated with worse overall survival, distinct immune infiltration patterns, and altered pathway activities.</div></div><div><h3>Conclusion</h3><div>This integrative study reveals the substantial role of HSPs, especially HSPA1L, in the pathogenesis and prognosis of PD and glioma. Our findings offer new perspectives on the molecular mechanisms underlying these diseases and propose HSPA1L as a potential prognostic biomarker and a target for therapeutic intervention.</div></div>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of a fully automated flow cytometry system with high robustness into a Screening Station. 将具有高稳定性的全自动流式细胞仪系统集成到筛选站中。

IF 2.5 4区医学

SLAS Technology Pub Date : 2024-10-23 DOI: 10.1016/j.slast.2024.100215

Shingo Fujiyama, Hidemitsu Asano, Ichiji Namatame

{"title":"Integration of a fully automated flow cytometry system with high robustness into a Screening Station.","authors":"Shingo Fujiyama, Hidemitsu Asano, Ichiji Namatame","doi":"10.1016/j.slast.2024.100215","DOIUrl":"10.1016/j.slast.2024.100215","url":null,"abstract":"<p><p>In recent years, there has been an increasing demand for the detection of rare cells in drug discovery research, such as cells that have differentiated off-purpose or are required for immunogenicity evaluation. Since detection and quantification limits depend on the robustness of the experiment, inter-human differences in technique have a significant impact on the performance of the assay system. Here, we integrated flow cytometry into a cell experiment platform, Screening Station, to construct a robust assay system, examined each step of the flow cytometric pretreatment using Jurkat cells, and finally evaluated the overall assay performance. Cell detection rate when the experiment was performed manually was 48.8 % ± 5.7 % (CV=11.6 %) versus 73.7 %±2.0 % (CV=2.8 %) with the automated method. To further clarify the analytical performance of the automated method, 1-100 PD-1 expressing Jurkat cells were spiked with 1 × 10<sup>5</sup> Jurkat cells, and the lower limit of detection, linearity, and CV% were evaluated. Average detection rate was 69 %, decision count was 0.985, and lower limit of detection was 4 cells (0.004 %). We evaluated the CV% value of the number of detected cells per spiked cell and found our system to be highly robust, approximating a binomial distribution with a 69 % recovery rate. In conclusion, we have integrated the Novocyte flow cytometry system into an automated experimental platform, Screening Station, to create a fully automated flow cytometric assay system with high robustness. Our platform can fulfill the technology needs of drug discovery for rare cell detection, which have intensified in recent years.</p>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accelerating covalent binding studies: Direct mass shift measurement with acoustic ejection and TOF-MS 加速共价结合研究：利用声发射和 TOF-MS 直接测量质量位移。

IF 2.5 4区医学

SLAS Technology Pub Date : 2024-10-23 DOI: 10.1016/j.slast.2024.100216

引用次数: 0

High-resolution acoustic ejection mass spectrometry for high-throughput library screening 用于高通量文库筛选的高分辨率声发射质谱仪

IF 2.5 4区医学

SLAS Technology Pub Date : 2024-10-19 DOI: 10.1016/j.slast.2024.100199

{"title":"High-resolution acoustic ejection mass spectrometry for high-throughput library screening","authors":"","doi":"10.1016/j.slast.2024.100199","DOIUrl":"10.1016/j.slast.2024.100199","url":null,"abstract":"<div><div>An approach is described for high-throughput quality assessment of drug candidate libraries using high-resolution acoustic ejection mass spectrometry (AEMS). Sample introduction from 1536-well plates is demonstrated for this application using 2.5 nL acoustically dispensed sample droplets into an Open Port Interface (OPI) with pneumatically assisted electrospray ionization at a rate of one second per sample. Both positive and negative ionization are shown to be essential to extend the compound coverage of this protease inhibitor-focused library. Specialized software for efficiently interpreting this data in 1536-well format is presented. A new high-throughput method for quantifying the concentration of the components (HTQuant) is proposed that neither requires adding an internal standard to each well nor further encumbers the high-throughput workflow. This approach for quantitation requires highly reproducible peak areas, which is shown to be consistent within 4.4 % CV for a 1536-well plate analysis. An approach for troubleshooting the workflow based on the background ion current signal is also presented. The AEMS data is compared to the industry standard LC/PDA/ELSD/MS approach and shows similar coverage but at 180-fold greater throughput. Despite the same ionization process, both methods confirmed the presence of a small percentage of compounds in wells that the other did not. The data for this relatively small, focused library is compared to a larger, more chemically diverse library to indicate that this approach can be more generally applied beyond this single case study. This capability is particularly timely considering the growing implementation of artificial intelligence strategies that require the input of large amounts of high-quality data to formulate predictions relevant to the drug discovery process. The molecular structures of the 872-compound library analyzed here are included to begin the process of correlating molecular structures with ionization efficiency and other parameters as an initial step in this direction.</div></div>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial intelligence-driven predictive framework for early detection of still birth 人工智能驱动的死胎早期检测预测框架。

IF 2.5 4区医学

SLAS Technology Pub Date : 2024-10-17 DOI: 10.1016/j.slast.2024.100203

{"title":"Artificial intelligence-driven predictive framework for early detection of still birth","authors":"","doi":"10.1016/j.slast.2024.100203","DOIUrl":"10.1016/j.slast.2024.100203","url":null,"abstract":"<div><div>Predictive modeling is becoming increasingly popular in the context of early disease detection. The use of machine learning approaches for predictive modeling can help early detection of diseases thereby enabling medical experts to appropriate medical treatments. Stillbirth prediction is a similar domain where artificial intelligence-based predictive modeling can alleviate this significant global health challenge. Despite advancements in prenatal care, the prevention of stillbirths remains a complex issue requiring further research and interventions. The cardiotocography (CTG) dataset is used in this research work from the UCI machine learning (ML) repository to investigate the efficiency of the proposed approach regarding stillbirth prediction. This research work adopts the Tabular Prior Data Fitted Network (TabPFN) model which was originally designed to solve small tabular classification. TabPFN is used to predict the still or live birth during pregnancy with 97.91% accuracy. To address this life-saving problem with more accurate results and in-depth analysis of ML models, this research work makes use of 13 renowned ML models for performance comparison with the proposed model. The proposed model is evaluated using precision, recall, F-score, Mathews Correlation Coefficient (MCC), and the area under the curve evaluation parameters and the results are 97.87%, 98.26%, 98.05%, 96.42%, and 98.88%, respectively. The results of the proposed model are further evaluated using k-fold cross-validation and its performance comparison with other state-of-the-art studies indicating the superior performance of TabPFN model.</div></div>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between asymmetry of transverse sinus and difference in intraocular pressure Based on MRV imaging examination 横窦不对称与眼压差异之间的关系基于 MRV 成像检查

IF 2.5 4区医学

SLAS Technology Pub Date : 2024-10-12 DOI: 10.1016/j.slast.2024.100202

{"title":"Relationship between asymmetry of transverse sinus and difference in intraocular pressure Based on MRV imaging examination","authors":"","doi":"10.1016/j.slast.2024.100202","DOIUrl":"10.1016/j.slast.2024.100202","url":null,"abstract":"<div><div>Transverse sinus asymmetry refers to the inconsistencies in the shape structure, size or blood flow of the intracranial transverse sinus. Intraocular pressure difference refers to the obvious difference in intraocular pressure between the two eyes. Transversal sinus asymmetry may be correlated with intraocular pressure difference, but the mechanism of correlation is still unclear. To investigate the relationship between transverse sinus asymmetry and IOP differences based on MRV examination, and to explore the possible mechanism. Patients with transverse sinus asymmetry were selected and examined using the MRV technique. At the same time, the patients' IOP was measured using standard methods of IOP measurement. Correlation analysis and statistical methods were used to evaluate the association between transverse sinus asymmetry and IOP differences. There was a statistically significant distinction observed between groups I and V (<em>Z</em> = 6.78, <em>P</em> < 0.01). Significant variations were also noted in the intraocular pressures across all groups, encompassing the average measurements of the right eye and left eye, along with the variance between the two (right eye: <em>F</em> = 15.43, <em>P</em> < 0.01; left eye: <em>F</em> = 4.62, <em>P</em> = 0.002; variance between eyes: <em>F</em> = 41.79, <em>P</em> < 0.01). The asymmetry of the transverse sinus exhibited a negative relationship with the intraocular pressure of the right eye (<em>r</em> = 0.51, <em>P</em> < 0.01) and the difference between the pressures of the two eyes (<em>r</em> = 0.79, <em>P</em> < 0.01); no significant association was found between the asymmetry and the left eye's intraocular pressure. In conclusion, a certain correlation exists between the intraocular pressures of the left and right eyes and the morphology of the transverse sinus. When the transverse sinus is thicker on one side, the corresponding drainage veins are thicker, resulting in lower intraocular pressure on that same side.</div></div>","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142442601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0