SLAS Technology最新文献

Model-based interactive visualization for complex systems requirements and design in joint tests. 联合测试中复杂系统需求与设计的基于模型的交互式可视化。

IF 2.5 4区医学

SLAS Technology Pub Date : 2025-02-01 Epub Date: 2024-12-14 DOI: 10.1016/j.slast.2024.100231

Chao Sun, Ruo Wu Wu, Si Yuan Liu

引用次数: 0

Implementing enclosed sterile integrated robotic platforms to improve cell-based screening for drug discovery. 实施封闭式无菌集成机器人平台以改进基于细胞的药物发现筛选。

IF 2.5 4区医学

SLAS Technology Pub Date : 2025-02-01 Epub Date: 2024-12-05 DOI: 10.1016/j.slast.2024.100230

Alice Rockliffe, Lauren Wheeler, Kiran Lidhar, Arun Dhar, Michelle Pemberton, Richard Kasprowicz, Ceridwen Hopely, Jo Francis, Shane Marine, David Brierley, Lorna Suckling

引用次数: 0

Application of ATR-FTIR spectroscopy and multivariate statistical analysis in cancer diagnosis.

IF 2.5 4区医学

SLAS Technology Pub Date : 2025-02-01 DOI: 10.1016/j.slast.2025.100253

Ao Song, Wanli Yang, Jun Wang, Yisa Cai, Lizheng Cai, Nan Pang, Ruihua Yu, Zhikun Liu, Chao Yang, Feng Jiang

{"title":"Application of ATR-FTIR spectroscopy and multivariate statistical analysis in cancer diagnosis.","authors":"Ao Song, Wanli Yang, Jun Wang, Yisa Cai, Lizheng Cai, Nan Pang, Ruihua Yu, Zhikun Liu, Chao Yang, Feng Jiang","doi":"10.1016/j.slast.2025.100253","DOIUrl":"https://doi.org/10.1016/j.slast.2025.100253","url":null,"abstract":"Lung cancer is one of the most prevalent and lethal malignant tumors worldwide. Currently, clinical diagnosis primarily relies on chest X-ray examinations, histopathological analysis, and the detection of tumor markers in blood. However, each of these methods has inherent limitations. The current study aims to explore novel diagnostic approaches for lung cancer by employing attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy in conjunction with multiple machine learning models. Fourier transform infrared spectroscopy can detect subtle differences in the material structures that reflect the carcinogenic process between lung cancer tissues and normal tissues. By applying principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) to analyze infrared spectral data, these subtle differences can be amplified. The study revealed that the combination of spectral bands within the 3500-3000 cm-1 and 1600-1500 cm-1 ranges is particularly significant for differentiating between the two groups. Three classification models-Support Vector Machine (SVM), k-Nearest Neighbor (kNN), and Linear Discriminant Analysis (LDA)-were constructed for spectral analysis of various band combinations. The results indicated that in detecting lung cancer samples, the combination of the 3500-3000 cm-1 and 1600-1500 cm-1 bands offers significant advantages. The analysis of the receiver operating characteristic (ROC) curve demonstrated that the area under the curve (AUC) exceeded 0.95 for all models, with the LDA model achieving an accuracy rate of 99.4% in identifying lung cancer patients compared to healthy individuals. The findings suggest that the integration of ATR-FTIR spectroscopy with multiple machine learning models represents a promising auxiliary diagnostic method for clinical lung cancer diagnosis, enabling detection at the molecular level.","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":" ","pages":"100253"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of vaginal microbiota on the pregnancy outcome of artificial insemination with husband's sperm based on microscope images combined with PCR fluorescence method. 基于显微镜图像结合PCR荧光法观察阴道微生物群对丈夫精子人工授精妊娠结局的影响。

IF 2.5 4区医学

SLAS Technology Pub Date : 2025-02-01 Epub Date: 2024-12-24 DOI: 10.1016/j.slast.2024.100242

Yanling Xing, Wei Wang, Na Wan, Dongmei Zhang, Mei Shan, Guan Wang

{"title":"The influence of vaginal microbiota on the pregnancy outcome of artificial insemination with husband's sperm based on microscope images combined with PCR fluorescence method.","authors":"Yanling Xing, Wei Wang, Na Wan, Dongmei Zhang, Mei Shan, Guan Wang","doi":"10.1016/j.slast.2024.100242","DOIUrl":"10.1016/j.slast.2024.100242","url":null,"abstract":"The vaginal microbiota plays an important role in reproductive health, especially in the process of artificial insemination. The imbalance of microbiota may affect pregnancy outcomes. Therefore, this study aims to explore the composition of vaginal microbiota and its impact on artificial insemination pregnancy outcomes, combined with microscopic images and PCR fluorescence methods, in order to provide scientific basis for improving the pregnancy outcomes of husband sperm artificial insemination. This study included female patients who underwent artificial insemination with husband's sperm and collected reproductive tract samples. By observing the microbial community morphology in the sample under a microscope and analyzing microbial DNA using PCR fluorescence method, the microbial ecological status is evaluated. Among women with successful pregnancy, the proportion of beneficial bacteria (such as lactic acid bacteria) is higher, while the abundance of pathogenic bacteria is significantly reduced. Specific microbial markers detected by PCR fluorescence method are positively correlated with pregnancy rate. Therefore, the microecological state of the female reproductive tract has a significant impact on the pregnancy outcome of artificial insemination. Maintaining a good microecological balance, especially increasing the proportion of beneficial bacteria, can help improve the success rate of artificial insemination.","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":"30 ","pages":"100242"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced surface-enhanced raman scattering nanoprobes for precise detection of Nitroreductase in Hypoxic tumor cells: Improving Cancer diagnosis. 先进的表面增强拉曼散射纳米探针用于缺氧肿瘤细胞中硝基还原酶的精确检测：提高癌症诊断。

IF 2.5 4区医学

SLAS Technology Pub Date : 2025-02-01 Epub Date: 2024-12-03 DOI: 10.1016/j.slast.2024.100229

Xiaoyue Zhao, Ying Zhang, Chunyan Zhu, Zhihui Yang, Xiaoyuan Chu

{"title":"Advanced surface-enhanced raman scattering nanoprobes for precise detection of Nitroreductase in Hypoxic tumor cells: Improving Cancer diagnosis.","authors":"Xiaoyue Zhao, Ying Zhang, Chunyan Zhu, Zhihui Yang, Xiaoyuan Chu","doi":"10.1016/j.slast.2024.100229","DOIUrl":"10.1016/j.slast.2024.100229","url":null,"abstract":"Nitroreductase (NTR) plays a critical role in the oxygen-deficient environment of anoxic tumor cells, and its identification is crucial for the diagnosis and treatment of cancer. This research introduces an innovative Surface Enhanced Raman Scattering (SERS) probe, created by attaching p-nitrothiophenol (p-NTP) to gold nanoparticles (Au NPs). This probe leverages the specific enzymatic reaction of NTR in hypoxic status, utilizing decreased NADH. The enzymatic activity of NTR transforms nitroaromatic compounds into aromatic amines, which is then reflected as a measurable shift in the SERS signal of the probe. This novel approach allows for the accurate quantification of NTR, with the sensitivity reaching a detection threshold of less than 0.02 μg/mL. The probe's non-toxic nature and superior biocompatibility facilitate its use for direct SERS investigations in A549 cells under reduced oxygen levels. We also applied this method to xenograft model. The results demonstrate a marked increase in NTR levels in tumor cells and tumor tissues in hypoxic conditions, highlighting the significance of this nanoprobe in enhancing cancer diagnostics, helping medical doctors making treatment decisions more swiftly and effectively.","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":" ","pages":"100229"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How to convert a 3D printer to a personal automated liquid handler for life science workflows. 如何将3D打印机转换为生命科学工作流程的个人自动液体处理器。

IF 2.5 4区医学

SLAS Technology Pub Date : 2025-02-01 Epub Date: 2024-12-26 DOI: 10.1016/j.slast.2024.100239

Dulguunnaran Naranbat, Benjamin Phelps, John Murphy, Anubhav Tripathi

{"title":"How to convert a 3D printer to a personal automated liquid handler for life science workflows.","authors":"Dulguunnaran Naranbat, Benjamin Phelps, John Murphy, Anubhav Tripathi","doi":"10.1016/j.slast.2024.100239","DOIUrl":"10.1016/j.slast.2024.100239","url":null,"abstract":"Automated liquid handlers are fundamental in modern life science laboratories, yet their high costs and large footprints often limit accessibility for smaller labs. This study presents an innovative approach to decentralizing a liquid handling system by converting a low-cost 3D printer into a customizable and accurate liquid handler. The Personal Automated Liquid Handler (PALH) system, costing ∼$400, incorporates a single-channel pipet, custom 3D-printed components, and open-source software for personalized workflows, allowing researchers to build and modify the system for specific experimental needs. The PALH system was evaluated through common life science assays, including preparing real-time PCR samples, end-point PCR with novel pipet-based downstream purification, and genomic DNA extraction from peripheral whole blood. In real-time PCR experiments targeting the YWHAZ gene, the PALH system demonstrated comparable performance to manual preparation across DNA quantities (1 pg to 100 ng). For end-point PCR, the PALH successfully amplified and purified 204 bp and 406 bp amplicons from a pUC19 vector, yielding concentrations similar to manual methods (5.43 ± 0.85 ng/µL vs. 2.10 ± 0.16 ng/µL for 204 bp; 3.74 ± 2.13 ng/µL vs. 1.51 ± 0.15 ng/µL for 406 bp, respectively). In genomic DNA extraction from whole blood, the PALH system achieved comparable DNA yields to manual extraction (49.52 ± 3.13 ng/µL vs. 48.62 ± 5.9 ng/µL), although at higher purity (260/280 ratio of 1.83 ± 0.07 vs. 1.92 ± 0.03), although both are at acceptable ranges. The open-source nature of the PALH system hopefully encourages further community-driven improvements and protocol sharing, fostering innovation and collaboration within the scientific community. As laboratory automation advances, the PALH system could be crucial in democratizing access to high-quality automated liquid handling, particularly in resource-limited settings.","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":" ","pages":"100239"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early Detection of Bronchopulmonary Dysplasia (BPD) in Preterm Infants Using Doppler Ultrasound Technology.

IF 2.5 4区医学

SLAS Technology Pub Date : 2025-01-30 DOI: 10.1016/j.slast.2025.100249

Pin Wang, Lihong Duan, Congxin Sun, Yu Chen, Yanyan Peng, Guihong Chen, Lixia Wu, Yan Li

{"title":"Early Detection of Bronchopulmonary Dysplasia (BPD) in Preterm Infants Using Doppler Ultrasound Technology.","authors":"Pin Wang, Lihong Duan, Congxin Sun, Yu Chen, Yanyan Peng, Guihong Chen, Lixia Wu, Yan Li","doi":"10.1016/j.slast.2025.100249","DOIUrl":"https://doi.org/10.1016/j.slast.2025.100249","url":null,"abstract":"Objective: The purpose of this research is to determine if Doppler ultrasonography technology may be used to forecast the onset of bronchopulmonary dysplasia (BPD) in premature babies in the early postnatal stage.Methods: On days 3, 7, 14, and 28 after delivery, Doppler ultrasonography exams were performed on a prospective cohort of 170 preterm newborns. Measurements were made of parameters such right ventricular output (RVO), systolic-to-diastolic ratio (S/D ratio), pulmonary artery acceleration time (PAAT), and velocity time integral (VTI). The predictive value of these indicators was evaluated using Kaplan-Meier survival analysis and multivariate logistic regression.Results: The severity of BPD was substantially correlated with all assessed Doppler ultrasound parameters (P<0.05). With an area under the curve (AUC) of 0.76, PAAT in particular demonstrated a reasonable capacity for prediction. A considerably greater cumulative incidence was found by Kaplan-Meier survival analysis.Conclusion: In conclusion, Doppler ultrasonography technology is a useful technique for identifying BPD in premature babies early on. High-risk babies can be efficiently identified by PAAT and VTI in particular, allowing for prompt intervention and possibly better results.","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":" ","pages":"100249"},"PeriodicalIF":2.5,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fully Automated Extraction of High-Quality total Nucleic Acids from FFPE specimens for Comprehensive Genomic Profiling of solid Tumors.

IF 2.5 4区医学

SLAS Technology Pub Date : 2025-01-30 DOI: 10.1016/j.slast.2025.100252

Kristopher Amirault, Michael Collins, Luca Beker, Brandon Mills, Martina Werner, Jonathan Andreas, Daniel Hartman, Jordan Dargert, Vanessa Process, Sean Cederlund, Thuy Dao, Linnea Menin, Molly Ferrara, Andrew Briggs, Joshua Shreve, Daniel Metzger, Angela Stout, Erin Deblasi, Jie An, Taylor Jensen, Shengle Zhang, Vinesh Rana, Bryn Burlingame, Ulrich Thomann, Durga Prasad Dash, Sha Liao, Jeffrey M Conroy, Eugenio Daviso

{"title":"Fully Automated Extraction of High-Quality total Nucleic Acids from FFPE specimens for Comprehensive Genomic Profiling of solid Tumors.","authors":"Kristopher Amirault, Michael Collins, Luca Beker, Brandon Mills, Martina Werner, Jonathan Andreas, Daniel Hartman, Jordan Dargert, Vanessa Process, Sean Cederlund, Thuy Dao, Linnea Menin, Molly Ferrara, Andrew Briggs, Joshua Shreve, Daniel Metzger, Angela Stout, Erin Deblasi, Jie An, Taylor Jensen, Shengle Zhang, Vinesh Rana, Bryn Burlingame, Ulrich Thomann, Durga Prasad Dash, Sha Liao, Jeffrey M Conroy, Eugenio Daviso","doi":"10.1016/j.slast.2025.100252","DOIUrl":"https://doi.org/10.1016/j.slast.2025.100252","url":null,"abstract":"Comprehensive Genomic Profiling (CGP) has emerged as a progressive standard of care for the understanding clinical oncology and treatment of solid tumors (Conroy, Pabla et al. 2021). By identifying actionable mutations through next-generation sequencing of solid tumors CGP enables targeted therapy decisions. Formalin-fixed paraffin-embedded tissues are notoriously difficult samples in respect to reliably extracting high-quality nucleic acids and sufficient genetic material to meet sequencing input requirements. In this work we present an automated solution for upstream sample processing of solid tumors to enable high throughput and scalable CGP workflows by eliminating bottlenecks and enhancing results. The Sonication STAR automated DNA and RNA methods from FFPE Tissues was created as a collaboration between Hamilton Company, Covaris, and Labcorp and it offers the potential reduction in quantity not sufficient (QNS) samples and sequencing performance improvements resulting in a 16% increase in fully reported tumor profiles for patients. This automated approach offers potential saving in workflow costs, improved efficiency, and a reduction in re-extraction and re-sequencing of tumors.","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":" ","pages":"100252"},"PeriodicalIF":2.5,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life Sciences Discovery and Technology Highlights.

IF 2.5 4区医学

SLAS Technology Pub Date : 2025-01-24 DOI: 10.1016/j.slast.2025.100248

Jamien Lim, Tal Murthy

引用次数: 0

Clinical Observation of VR Virtual Reality Rehabilitation Training Combined with Acupuncture in the Treatment of Mild Cognitive Impairment.

IF 2.5 4区医学

SLAS Technology Pub Date : 2025-01-23 DOI: 10.1016/j.slast.2025.100250

Ying Fu, Hui Wang

{"title":"Clinical Observation of VR Virtual Reality Rehabilitation Training Combined with Acupuncture in the Treatment of Mild Cognitive Impairment.","authors":"Ying Fu, Hui Wang","doi":"10.1016/j.slast.2025.100250","DOIUrl":"https://doi.org/10.1016/j.slast.2025.100250","url":null,"abstract":"Mild cognitive impairment (MCI) is an intermediate state between normal aging and dementia, and its symptoms include easy forgetting, distraction, and mental deterioration. This directly affects the patient's motor function, daily living ability, and social adaptability, and brings many difficulties to the patient's reintegration into society. Therefore, clinical research on MCI is very necessary. The purpose of this paper is to study the efficacy of acupuncture and moxibustion in the treatment of MCI based on VR virtual reality technology. This paper firstly expounds the application prospect of VR virtual reality rehabilitation training and virtual scene intervention in MCI patients. It then introduces the characteristics of acupuncture and the acupuncture points of scalp acupuncture and eye acupuncture. This paper then establishes the algorithm model of the VR virtual reality rehabilitation training system, which lays a solid foundation for the design of the subsequent rehabilitation training system. This paper then conducts clinical experiments and analysis on the treatment of mild cognitive impairment, which establishes a treatment group and a control group for comparative experiments. The experimental results showed that the combined effect size OR=2.74 of the meta-analysis, the combined effect size test statistic Z=3.38, WMD=2.42, and the 95% CI was (1.98, 3.06). It indicated that the treatment group had better efficacy. The MMSE score and MoCA score of the treatment group after treatment and training increased by 28.68% and 15.36%, respectively, and the scores were higher than those of the control group. Therefore, the use of VR virtual reality technology combined with acupuncture is of great significance for the rehabilitation and treatment of patients with mild cognitive impairment.","PeriodicalId":54248,"journal":{"name":"SLAS Technology","volume":" ","pages":"100250"},"PeriodicalIF":2.5,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0