npj Regenerative Medicine最新文献_第6页

Circulating beta-2-microglobulin promotes revascularization via TGFBR2 after spinal cord injury. 脊髓损伤后循环β -2微球蛋白通过TGFBR2促进血运重建。

IF 6.5 1区医学

npj Regenerative Medicine Pub Date : 2025-12-29 DOI: 10.1038/s41536-025-00451-w

Hiroshi Yamagishi, Akiko Uyeda, Lili Quan, Hidemi Misawa, Rieko Muramatsu

{"title":"Circulating beta-2-microglobulin promotes revascularization via TGFBR2 after spinal cord injury.","authors":"Hiroshi Yamagishi, Akiko Uyeda, Lili Quan, Hidemi Misawa, Rieko Muramatsu","doi":"10.1038/s41536-025-00451-w","DOIUrl":"10.1038/s41536-025-00451-w","url":null,"abstract":"Vascular network disruption caused by spinal cord injury (SCI) exacerbates secondary neuronal damage. Although vascular barrier disruption naturally restores over time, its underlying mechanism is not fully clarified. Here, we found that blood factors promote the proliferation of endothelial cells (ECs), which are essential for revascularization in the repair process after SCI. In vivo, endogenous IgG leakage into the spinal cord initiates EC proliferation at the lesion after injury. In vitro, adult mouse serum promotes mouse ECs proliferation through beta-2-microglobulin (B2M) via transforming growth factor beta receptor 2 (TGFBR2). Under EC-specific knockdown of Tgfbr2 in vivo, we observed exacerbated blood extravasation and increased inflammatory cell infiltration compared to controls. Additionally, suppression of endothelial Tgfbr2 impaired motor function recovery, axon regrowth, and regeneration in injured mice. These findings suggest that targeting the B2M-TGFBR2 axis could be a potential therapeutic approach to promoting functional recovery against vascular disruption after SCI.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":" ","pages":"7"},"PeriodicalIF":6.5,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12864802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145859057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term evaluation of human iPSC-derived cartilage for repairing chondral defects. 人ipsc来源软骨修复软骨缺损的长期评价。

IF 6.5 1区医学

npj Regenerative Medicine Pub Date : 2025-12-25 DOI: 10.1038/s41536-025-00447-6

Yangfan Lu, Elizabeth R Kats, Sophie E Hines, Jiangyinzi Shang, Shotaro Kamijo, Jia-Jun Liu, Silvia Liu, MaCalus V Hogan, Hang Lin

引用次数: 0

Emerging concepts and novel mechanisms in organ fibrosis. 器官纤维化的新概念和新机制。

IF 6.5 1区医学

npj Regenerative Medicine Pub Date : 2025-12-17 DOI: 10.1038/s41536-025-00446-7

Adrián Ruiz-Villalba, Ana Pardo-Saganta

引用次数: 0

Deer antler ASCs exosomes ameliorate osteoarthritis via miR-140/MMP13 axis-mediated dual modulation of inflammation and cartilage regeneration. 鹿茸ASCs外泌体通过miR-140/MMP13轴介导的炎症和软骨再生双重调节改善骨关节炎。

IF 6.5 1区医学

npj Regenerative Medicine Pub Date : 2025-12-08 DOI: 10.1038/s41536-025-00444-9

Yuhao Song, Xue Wang, Xinrui Yan, Xin Li, Xintong Han, Yu Zhang, Yusu Wang, Xinran Chen, Xinyi Li, Xinyu Zhang, Boyin Jia, Rui Du

{"title":"Deer antler ASCs exosomes ameliorate osteoarthritis via miR-140/MMP13 axis-mediated dual modulation of inflammation and cartilage regeneration.","authors":"Yuhao Song, Xue Wang, Xinrui Yan, Xin Li, Xintong Han, Yu Zhang, Yusu Wang, Xinran Chen, Xinyi Li, Xinyu Zhang, Boyin Jia, Rui Du","doi":"10.1038/s41536-025-00444-9","DOIUrl":"10.1038/s41536-025-00444-9","url":null,"abstract":"Osteoarthritis (OA) is a progressive joint disease characterized by cartilage degeneration. Although the current use of mesenchymal stromal cells (MSCs) treatment provides a novel therapeutic option, stem cell therapy is limited to the risk of immune rejection, and stem cell-derived extracellular vesicles (Exos) are emerging as a more potential choice. Antler is a truly regenerative organ with unprecedented regenerative capacity and chondrogenic potential, and its derived antler stem cells (ASCs) provide a unique and sustainable biological resource for obtaining bioactive ASC-Exos. In this study, we found that intra-articular injection of ASC-Exos can effectively promote cartilage repair. Further analysis indicated that the key functional component of these exosomes is mir-140, which functions by regulating its target, matrix metalloproteinase 13 (MMP13). Finally, we found that miR-140-engineered ASC-Exo promotes chondrocyte activity, reduces apoptosis both in vitro and in vivo, and alleviates inflammation while inhibiting cartilage matrix degradation. Therefore, this study provides a new regenerative medical strategy for the treatment of osteoarthritis.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":" ","pages":"1"},"PeriodicalIF":6.5,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12796405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145710310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A ferret model demonstrates sustained fibrosis, restrictive physiology, and airway proximalization. 雪貂模型显示持续纤维化，限制性生理和气道近端化。

IF 7 1区医学

npj Regenerative Medicine Pub Date : 2025-11-27 DOI: 10.1038/s41536-025-00436-9

Jacelyn E Peabody Lever, Qian Li, Nikoleta Pavelkova, Shah S Hussain, Sayan Bakshi, Janna Q Ren, Luke I Jones, Kajal Jadhav, Jared P Kennemur, Mason Weupe, Javier Campos-Gomez, Liping Tang, Jeremie M Peabody Lever, Dezhi Wang, Denise D Stanford, Jeremy B Foote, Kevin S Harrod, Harrison Kim, Scott E Phillips, Steven M Rowe

{"title":"A ferret model demonstrates sustained fibrosis, restrictive physiology, and airway proximalization.","authors":"Jacelyn E Peabody Lever, Qian Li, Nikoleta Pavelkova, Shah S Hussain, Sayan Bakshi, Janna Q Ren, Luke I Jones, Kajal Jadhav, Jared P Kennemur, Mason Weupe, Javier Campos-Gomez, Liping Tang, Jeremie M Peabody Lever, Dezhi Wang, Denise D Stanford, Jeremy B Foote, Kevin S Harrod, Harrison Kim, Scott E Phillips, Steven M Rowe","doi":"10.1038/s41536-025-00436-9","DOIUrl":"10.1038/s41536-025-00436-9","url":null,"abstract":"Unlike rodents, ferrets have human-like distribution of submucosal glands expressing MUC5B, associated with idiopathic pulmonary fibrosis (IPF). We evaluated ferrets exposed to a single dose of bleomycin (5 U/kg) longitudinally, and found sustained restrictive physiology, increased opacification and fibrotic injury in the lungs through 22 weeks. Notably, these lungs had an aberrant \"proximalization\" repair phenotype indicated by increased proportion of smaller airways co-expressing club cell secretory protein and alpha-tubulin that was associated with extent of fibrotic injury. We also observed MUC5B-positive cystic structures in the distal lung suggestive of honeycombing, consistent with increase of MUC5B+ airways in combination with a size shift to smaller airways. We conclude that ferrets exhibit aberrant repair and pathologic features characteristic of human IPF, including proximalization of the distal airways that has not been recapitulated in rodents. Heightened MUC5B expression may play a key role in promoting airway remodeling and sustained lung injury in IPF.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"55"},"PeriodicalIF":7.0,"publicationDate":"2025-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12660791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145642626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-free in-line characterization of immune cell culture using quantitative phase imaging. 使用定量相位成像的免疫细胞培养的无标记在线表征。

IF 6.5 1区医学

npj Regenerative Medicine Pub Date : 2025-11-27 DOI: 10.1038/s41536-025-00442-x

Caroline E Serafini, Viswanath Gorti, Paloma Casteleiro Costa, Aaron D Silva Trenkle, Bharat Kanwar, Bryan Wang, Brian Wicker, Linda E Kippner, Isaac LeCompte, Rui Qi Chen, Benjamin Joffe, Ye Li, Annie C Bowles-Welch, Jing Li, Christine E Brown, Gabriel A Kwong, Stephen Balakirsky, Krishnendu Roy, Francisco E Robles

{"title":"Label-free in-line characterization of immune cell culture using quantitative phase imaging.","authors":"Caroline E Serafini, Viswanath Gorti, Paloma Casteleiro Costa, Aaron D Silva Trenkle, Bharat Kanwar, Bryan Wang, Brian Wicker, Linda E Kippner, Isaac LeCompte, Rui Qi Chen, Benjamin Joffe, Ye Li, Annie C Bowles-Welch, Jing Li, Christine E Brown, Gabriel A Kwong, Stephen Balakirsky, Krishnendu Roy, Francisco E Robles","doi":"10.1038/s41536-025-00442-x","DOIUrl":"10.1038/s41536-025-00442-x","url":null,"abstract":"Cell therapies, such as T cell immunotherapies, hold significant promise for treating complex diseases; however, their widespread adoption has been hindered by challenges related to monitoring cells during culture, which has affected their consistency, potency, and cost. Here, we present a compact, low-cost, label-free quantitative phase imaging (QPI) platform to enable continuous, non-destructive, in-line monitoring of T cell cultures within bioreactors. We further develop quantitative, image-based assays that accurately characterizes T cell culture viability and activation from over 50 independent donors-including therapeutically relevant CAR-T cells - while also preserving culture sterility and eliminating the need for disruptive sampling and endpoint assays. Our findings establish a QPI-pipeline for label-free, in-line cell monitoring and characterization which can significantly improve cell manufacturing processes.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"56"},"PeriodicalIF":6.5,"publicationDate":"2025-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12660828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145642651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulating Schwann cell behavior via functional nerve guidance conduits for enhanced peripheral nerve regeneration. 通过功能性神经引导导管调节雪旺细胞行为增强周围神经再生。

IF 6.5 1区医学

npj Regenerative Medicine Pub Date : 2025-11-26 DOI: 10.1038/s41536-025-00443-w

Keyue Yang, Shujian Yang, Xiaofeng Teng, Xinkun He, Tao Sun, Hong Chen

引用次数: 0

Ferret model of bleomycin-induced lung injury shares features of human idiopathic pulmonary fibrosis. 博莱霉素诱导的雪貂肺损伤模型具有人类特发性肺纤维化的特征。

IF 6.5 1区医学

npj Regenerative Medicine Pub Date : 2025-11-21 DOI: 10.1038/s41536-025-00440-z

Shuang Wu, Ian Driver, Meihui Luo, Hikaru Miyazaki, Smitha Shambhu, Dimitry Popov, Liyuan Yang, Jing Wang, Jia Ma, Junfeng Guo, Jarron Atha, Aleksandra Tata, Eric A Hoffman, Yujiong Wang, Purushothama Rao Tata, Martin B Jensen, John F Engelhardt, Vishwaraj Sontake, Xiaoming Liu

{"title":"Ferret model of bleomycin-induced lung injury shares features of human idiopathic pulmonary fibrosis.","authors":"Shuang Wu, Ian Driver, Meihui Luo, Hikaru Miyazaki, Smitha Shambhu, Dimitry Popov, Liyuan Yang, Jing Wang, Jia Ma, Junfeng Guo, Jarron Atha, Aleksandra Tata, Eric A Hoffman, Yujiong Wang, Purushothama Rao Tata, Martin B Jensen, John F Engelhardt, Vishwaraj Sontake, Xiaoming Liu","doi":"10.1038/s41536-025-00440-z","DOIUrl":"10.1038/s41536-025-00440-z","url":null,"abstract":"Idiopathic pulmonary fibrosis (IPF) is a devastating lung disease with limited treatment options, partly due to a lack of effective disease models. This study presents a ferret model of pulmonary fibrosis (PF) induced by bleomycin, which replicates key characteristics of human IPF. The ferret model demonstrates an irreversible loss of pulmonary compliance, increased opacification, and structures resembling honeycomb cysts. Using single-nucleus RNA sequencing, we observed a significant shift in the distal lung epithelium toward a proximal phenotype. Cell trajectory analysis showed that AT2 cells transition into KRT8high/KRT7low/SOX4+ cells, and eventually into KRT8high/KRT7high/SFN+/TP63+/KRT5low \"basaloid-like\" cells. These cells, along with KRT7 and KRT8 populations, are located over myofibroblasts in fibrotic areas, suggesting a role in fibrosis progression similar to that in human IPF. This model accurately reproduces the pathophysiological and molecular features of human IPF, making it a valuable tool for future research and therapeutic development.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"53"},"PeriodicalIF":6.5,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12639051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145574828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long noncoding RNA LncBAR enhances BRG1 protein to promote cardiomyocyte cell cycle progression and cardiac repair. 长链非编码RNA LncBAR增强BRG1蛋白促进心肌细胞周期进程和心脏修复。

IF 6.5 1区医学

npj Regenerative Medicine Pub Date : 2025-11-17 DOI: 10.1038/s41536-025-00439-6

Jun Li, Huanhuan Cai, Yufan Chen, Ruiqi Pi, Lilin Xiang, Zhibing Lu, Yan Zhou, Li Wang

{"title":"Long noncoding RNA LncBAR enhances BRG1 protein to promote cardiomyocyte cell cycle progression and cardiac repair.","authors":"Jun Li, Huanhuan Cai, Yufan Chen, Ruiqi Pi, Lilin Xiang, Zhibing Lu, Yan Zhou, Li Wang","doi":"10.1038/s41536-025-00439-6","DOIUrl":"10.1038/s41536-025-00439-6","url":null,"abstract":"The mammalian heart retains regenerative capacity during the early postnatal period, but this ability declines as it matures. Enhancing cardiomyocyte proliferation represents a key therapeutic approach to promote heart regeneration and repair, yet the molecular mechanisms remain elusive. Here, we identified LncBAR (BAF complex-associated lncRNA) as a critical regulator of cardiac regeneration. LncBAR expression declines during heart development but is upregulated following cardiac injury. Loss of LncBAR impairs cardiomyocyte growth, suppresses cell cycle gene expression, and diminishes heart regeneration, as evidenced by reduced cytokinesis and cardiac function. Conversely, cardiac specific overexpression of LncBAR restores cardiomyocyte proliferation and enhances cardiac regeneration, especially in adult myocardial infarction model. Mechanistically, LncBAR interacts with Brg1, stabilizing BRG1 protein level and activating cell cycle progression to drive cardiomyocytes proliferation. Collectively, our study identified LncBAR as a crucial regulator for heart regeneration, highlighting the LncBAR-BRG1 axis as a promising therapeutic strategy for cardiac repair.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"52"},"PeriodicalIF":6.5,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12623768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145543908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human fingertip regeneration follows clinical phases with distinct proteomic signatures. 人类指尖再生遵循具有不同蛋白质组特征的临床阶段。

IF 6.5 1区医学

npj Regenerative Medicine Pub Date : 2025-11-05 DOI: 10.1038/s41536-025-00441-y

Jurek Schultz, Purva A Patel, Rita Aires, Leah Wissing, Patrick Glatte, Michael Seifert, Marc Gentzel, Guido Fitze, Adele M Doyle, Tatiana Sandoval-Guzmán

{"title":"Human fingertip regeneration follows clinical phases with distinct proteomic signatures.","authors":"Jurek Schultz, Purva A Patel, Rita Aires, Leah Wissing, Patrick Glatte, Michael Seifert, Marc Gentzel, Guido Fitze, Adele M Doyle, Tatiana Sandoval-Guzmán","doi":"10.1038/s41536-025-00441-y","DOIUrl":"10.1038/s41536-025-00441-y","url":null,"abstract":"Distal injuries in human fingertips can regenerate almost fully, yet the process of human fingertip regeneration has hardly been characterized on a cellular and molecular level. A silicone finger cap, comprising a puncturable reservoir, was used to treat 22 human fingertip amputations. In all patients, subcutaneous tissue, nailbed and skin regenerated with excellent outcomes. Through the clinical assessment of the wounds, the regenerative process was divided into four distinct phases. Proteomic data from wound fluid samples collected at regular intervals, confirmed robust and unbiasedly distinct proteomic signatures, characteristic processes, and active regulatory networks in each phase. Moreover, this human dataset provides important insights, showing clear divergences from findings in regenerative animal models. The longitudinal and comprehensive analysis presented here unveils the complex orchestration of four clinically and proteomically-distinct phases of human fingertip regeneration. Further analyses of this proteomic data will allow for the identification of candidates orchestrating human fingertip regeneration and serving as a framework for comparative and regenerative medicine studies. This clinical trial was registered at ClinicalTrials.gov Identifier: NCT03089060 on March 17, 2017.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"51"},"PeriodicalIF":6.5,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12589496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145454018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0