Mackenzie E Turner, Jingru Che, Joseph T Leland, Delaney J Villarreal, Sahana Rajesh, Sugath Suravarapu, Kan N Hor, Matthew G Wiet, Bryce A Kerlin, Tai Yi, Cameron A Best, James W Reinhardt, Christopher K Breuer
{"title":"Modulating the platelet-mediated innate foreign body response to affect in situ vascular tissue engineering outcomes.","authors":"Mackenzie E Turner, Jingru Che, Joseph T Leland, Delaney J Villarreal, Sahana Rajesh, Sugath Suravarapu, Kan N Hor, Matthew G Wiet, Bryce A Kerlin, Tai Yi, Cameron A Best, James W Reinhardt, Christopher K Breuer","doi":"10.1038/s41536-025-00419-w","DOIUrl":"https://doi.org/10.1038/s41536-025-00419-w","url":null,"abstract":"<p><p>The success of implanted tissue-engineered vascular grafts (TEVGs) relies on the coordinated inflammation and wound healing processes that simultaneously degrade the scaffold and guide the formation of a neovessel. Dysregulated responses can lead to aberrant remodeling (e.g., stenosis), impacting the long-term outcome and functionality of the TEVG. We developed a TEVG that, despite demonstrating growth capacity in the clinic, exhibited an unexpectedly high incidence of stenosis, or narrowing of the graft lumen. This study identified platelet-mediated immune signaling via the lysosomal trafficking regulator (Lyst) as a key driver of stenosis. Lyst mutations significantly impaired platelet dense granule exocytosis yet preserved alpha granule secretion and adhesion to the biomaterial. Uncontrolled platelet aggregation, potentiated by dense granule signaling, results in the formation of a mural thrombus that remodels into occlusive neotissue. Importantly, inhibiting sustained platelet aggregation using the P2Y12 antagonist, prasugrel, is a successful strategy for optimizing neotissue formation and improving overall TEVG performance.</p>","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"34"},"PeriodicalIF":6.4,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ni Su, Heena Saqib, Cassandra Villicana, Sungwon Kim, Thomas H Ambrosi, Peyton Freeman, Tayne Kim, Xinming Tong, Manish Ayushman, Charles K F Chan, Fan Yang
{"title":"Modulating immune-stem cell crosstalk enables robust bone regeneration via tuning compositions of macroporous scaffolds.","authors":"Ni Su, Heena Saqib, Cassandra Villicana, Sungwon Kim, Thomas H Ambrosi, Peyton Freeman, Tayne Kim, Xinming Tong, Manish Ayushman, Charles K F Chan, Fan Yang","doi":"10.1038/s41536-025-00421-2","DOIUrl":"10.1038/s41536-025-00421-2","url":null,"abstract":"<p><p>Following bone injury, macrophages (Mφ) initiate the immune response by secreting signals that recruit mesenchymal stem cells (MSC) and other niche cells to shape healing. Despite its importance, the potential of enhancing bone regeneration by modulating immune-stem cell crosstalk is largely unexplored. Here, we report a macroporous microribbon (µRB) scaffold with tunable ratios of gelatin (Gel) and chondroitin sulfate (CS), achieving rapid endogenous bone regeneration in a critical-sized defect model without exogenous growth factors or cells. The 3D MSC/Mφ co-culture model, but not the mono-culture model, effectively identified Gel50_CS50 as the leading ratio for accelerating bone regeneration in vivo. Single-cell sequencing (scRNAseq) and CellChat analysis revealed that Gel50_CS50 significantly enhanced the cellular crosstalk among Mφ and other bone niche cell types, with signaling pathways linked to anti-inflammation, angiogenesis, and osteogenesis. This study demonstrates Gel50_CS50 µRB as a promising biomaterial-based therapy for treating critical-sized bone defects by modulating cellular crosstalk.</p>","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"33"},"PeriodicalIF":6.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12219738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144546161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bioengineered chondrocyte-products from human induced pluripotent stem cells are useful for repairing articular cartilage injury in minipig model.","authors":"Shota Takihira, Tomoka Takao, Yuki Fujisawa, Daisuke Yamada, Shojiro Hanaki, Tomohiro Inoue, Shigeo Otake, Aki Yoshida, Kazuki Yamada, Shinichi Miyazawa, Eiji Nakata, Toshifumi Ozaki, Takeshi Takarada","doi":"10.1038/s41536-025-00420-3","DOIUrl":"10.1038/s41536-025-00420-3","url":null,"abstract":"<p><p>The capacity of articular cartilage for self-repair is limited. Therefore, wide-ranging cartilage damage rarely resolves spontaneously, leading to the development of osteoarthritis. Previously, we developed human-induced pluripotent stem cell (hiPSC)-derived expandable human limb-bud-like mesenchymal (ExpLBM) cells with stable expansion and high chondrogenic capacity. In this study, various forms of articular cartilage-like tissue were fabricated using ExpLBM technology and evaluated to examine their potential as biomaterials. ExpLBM cells derived from hiPSCs were used to produce particle-like cartilage tissue and plate-like cartilage tissue. The cartilaginous particles and cartilaginous plates were transplanted into a minipig osteochondral defect model, and cartilage engraftment was histologically evaluated. For both transplanted cartilaginous particles and cartilaginous plates, good Safranin O staining and integration with the surrounding tissue were observed. Cartilaginous particles and cartilaginous plates made using hiPSCs-derived ExpLBM cells are effective for the regeneration of cartilage after injury.</p>","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"31"},"PeriodicalIF":6.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12219148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144546160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marije Koning, Sébastien J Dumas, Elda Meta, Ellen Lievers, Annemarie M A de Graaf, Mila Borri, Lynn J Nai Chung Tong, Xue Liang, Ping Liu, Fang Chen, Lin Lin, Yonglun Luo, Peter Carmeliet, Cathelijne W van den Berg, Ton J Rabelink
{"title":"Single cell transcriptomics of human kidney organoid endothelium reveals vessel growth processes and arterial maturation upon transplantation.","authors":"Marije Koning, Sébastien J Dumas, Elda Meta, Ellen Lievers, Annemarie M A de Graaf, Mila Borri, Lynn J Nai Chung Tong, Xue Liang, Ping Liu, Fang Chen, Lin Lin, Yonglun Luo, Peter Carmeliet, Cathelijne W van den Berg, Ton J Rabelink","doi":"10.1038/s41536-025-00418-x","DOIUrl":"10.1038/s41536-025-00418-x","url":null,"abstract":"<p><p>Kidney organoids derived from human induced pluripotent stem cells lack a proper vasculature, hampering their applicability. Transplantation prevents the loss of organoid endothelial cells (ECs) observed in vitro, and promotes vascularization. In this study, we transplanted kidney organoids in chicken embryos and deployed single-cell RNA sequencing of ~12,000 organoid ECs to delineate their molecular landscape and identify key changes associated with transplantation. Transplantation significantly altered EC phenotypic composition. Consistent with angiogenesis, proliferating EC populations expanded 8 days after transplantation. Importantly, ECs underwent a major vein-to-arterial phenotypic shift. One of the transplantation-specific arterial EC populations, characterized by laminar shear stress response and Notch signalling, showed a similar transcriptome as human fetal kidney arterial/afferent arteriolar ECs. Consistently, transplantation-induced transcriptional changes involved proangiogenic and arteriogenic SOX7 transcription factor upregulation and regulon enrichment. These findings point to blood flow and candidate transcription factors such as SOX7 as possible targets to enhance kidney organoid vascularization.</p>","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"32"},"PeriodicalIF":6.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12216864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144546162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Direct reprogramming of mouse fibroblasts into self-renewable alveolar epithelial-like cells.","authors":"Atsuho Morita, Makoto Ishii, Takanori Asakura, Masaya Yotsukura, Ahmed E Hegab, Tatsuya Kusumoto, Ho Namkoong, Takunori Ogawa, Yuhki Nakatake, Mayumi Oda, Fumitake Saito, Hirofumi Kamata, Junko Hamamoto, Satoshi Okamori, Toshiki Ebisudani, Hiroyuki Yasuda, Shinya Sugimoto, Yuta Kuze, Masahide Seki, Yutaka Suzuki, Naoki Hasegawa, Hisao Asamura, Hideo Watanabe, Minoru Ko, Toshiro Sato, Masaki Ieda, Koichi Fukunaga","doi":"10.1038/s41536-025-00411-4","DOIUrl":"10.1038/s41536-025-00411-4","url":null,"abstract":"<p><p>Direct reprogramming is a breakthrough technology that can alter the fate of cells without the passage of stem cells. However, direct reprogramming of somatic cells into pulmonary alveolar epithelial cells has not yet been achieved. Here, we report the direct reprogramming of mouse tail tips and embryonic fibroblasts into induced pulmonary alveolar epithelial-like cells (iPULs) using four transcription factor-coding genes (Nkx2-1, Foxa1, Foxa2, and Gata6) and three-dimensional culture. The iPULs showed lamellar body-like structures and displayed key properties of pulmonary alveolar epithelial cells. Although the potential for iPULs to morphologically differentiate into alveolar epithelial type 1 cells was limited in vitro, the intratracheal administration of iPULs in a bleomycin-induced mouse model of pulmonary fibrosis led to their integration into the alveolar surface, where they formed both alveolar epithelial type 1 and type 2-like cells. Thus, reprogrammed fibroblasts may represent a new source of pulmonary alveolar epithelial cells for regenerative medicine.</p>","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"30"},"PeriodicalIF":6.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12185750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sydney Borcherding, Matthew D Wood, Sai L Pinni, Lauren Schellhardt, Anne E Faust, Marissa N Behun, Clint Skillen, Pooja Chawla, Mangesh Kulkarni, Elena A Demeter, Andrew D Miller, Mark A Mahan, Bryan N Brown, Lorenzo Soletti
{"title":"Prevention of nerve growth and evoked pain with a nerve cap graft device.","authors":"Sydney Borcherding, Matthew D Wood, Sai L Pinni, Lauren Schellhardt, Anne E Faust, Marissa N Behun, Clint Skillen, Pooja Chawla, Mangesh Kulkarni, Elena A Demeter, Andrew D Miller, Mark A Mahan, Bryan N Brown, Lorenzo Soletti","doi":"10.1038/s41536-025-00416-z","DOIUrl":"10.1038/s41536-025-00416-z","url":null,"abstract":"<p><p>Neuroma following nerve injury and/or amputation is a debilitating condition with significant impacts on quality of life. Several approaches exist to prevent or treat neuroma and/or reduce associated pain; however, these approaches are not consistently effective, facile, or widely accessible. The present study characterizes a xenogeneic nerve cap graft device (NCGD) composed of decellularized porcine nerve. The NCGD was assessed for its ability to inhibit nerve growth, neuroma formation, and pain in rodent models of sciatic neurectomy and tibial neuroma transposition. The NCGD provided a neuroinhibitory substrate that abated and interrupted nerve growth within 5 mm of the nerve stump and was progressively remodeled into healthy host-derived tissue. The NCGD also resulted in a 3.5-fold reduction in evoked pain and a decrease in pain-associated markers at the dorsal root ganglia. These results suggest that the NCGD may provide a simple and widely accessible alternative for prophylactic treatment of symptomatic neuroma.</p>","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"29"},"PeriodicalIF":6.4,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Justin A Varholick, Rishi Kondapaneni, Malcolm Maden
{"title":"Spiny mice (Acomys) regenerate wounded whisker pad skin with whisker follicles, muscles, and targeted innervation.","authors":"Justin A Varholick, Rishi Kondapaneni, Malcolm Maden","doi":"10.1038/s41536-025-00415-0","DOIUrl":"10.1038/s41536-025-00415-0","url":null,"abstract":"<p><p>Human skin is repaired by scar formation, lacking hair follicles, arrector pili muscles, and targeted innervation. Scarring leads to significant losses in skin functionality. Contrary to humans, spiny mice (Acomys spp.) repair skin via scar-free regeneration, regrowing hair follicles and muscles. However, skin across the body is diverse, and whether Acomys can regenerate specialized skin remains unclear. Here, we report that Acomys regenerated whisker pad skin with whisker follicles (i.e., vibrissae), blood sinuses, sebaceous glands, skeletal muscles, and targeted innervation. In contrast, CD-1 mice (Mus) healed via scarring and poor innervation of the scar. While whisker pad skin regeneration in Acomys was remarkable, only 20% of whiskers regenerated on average, ranging from 0% to 75%. Regenerated axons were bundled in epineurial sheaths, targeting the regenerated whisker, with an average of 75% of the uninjured innervation. This expands our understanding of Acomys skin regeneration and provides novel models for skin regeneration and sensorimotor recovery.</p>","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"28"},"PeriodicalIF":6.4,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137887/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kalliopi Arkoudi, Yue Yuan, Antonia Pia Cumine, Carlene Dyer, Elisabeth Busch-Nentwich, Isabel Bravo, Yi Feng, Robert D Knight
{"title":"An NF-kB/TNF-alpha signalling feedback loop acts to coordinate tissue regeneration and macrophage behaviour in zebrafish.","authors":"Kalliopi Arkoudi, Yue Yuan, Antonia Pia Cumine, Carlene Dyer, Elisabeth Busch-Nentwich, Isabel Bravo, Yi Feng, Robert D Knight","doi":"10.1038/s41536-025-00414-1","DOIUrl":"10.1038/s41536-025-00414-1","url":null,"abstract":"<p><p>Inflammatory cells are crucial regulators of infection and regeneration that actively migrate to affected tissues. NF-kB and TNF-alpha (TNFα) are master regulators of immune signalling, but their importance for immune cell migration is much less well understood. We have therefore investigated how NF-kB and TNFα regulate both macrophage function and behaviour in vivo using a zebrafish model of tissue repair. We show that NF-kB activity differentially regulates TNFα activity through Tnf receptors 1a and 1b to control macrophage responses to injury. Loss of NF-kB in macrophages results in elevated TNFα expression and results in more directional migration. Impaired NF-kB activity in macrophages perturbs tissue regeneration, causes increased proliferation, altered pro- and anti-inflammatory gene expression and delays fin regeneration. We identify a crucial role for NF-kB modulation of TNFα signaling to regulate macrophage responses to tissue injury, which are necessary for effective fin regeneration.</p>","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"27"},"PeriodicalIF":6.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12134371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zeji Du, Skylar A Rizzo, Tiffany L Sarrafian, Monique S Bagwell, Ryan C Mahlberg, Ashley Amontree, Paige Schiebel, Dinah M Tauferner, Zoe S LeBrasseur, Tyra A Witt, Mary Nagel, Kyla A Boyd, Humberto De Vitto, Matthew L Hillestad, Paul G Stalboerger, Matthew T Houdek, Rafael J Sierra, Atta Behfar
{"title":"Engineered BMP2/BMP7 extracellular vesicles induce autocrine BMP release driving SMAD phosphorylation to promote bone formation.","authors":"Zeji Du, Skylar A Rizzo, Tiffany L Sarrafian, Monique S Bagwell, Ryan C Mahlberg, Ashley Amontree, Paige Schiebel, Dinah M Tauferner, Zoe S LeBrasseur, Tyra A Witt, Mary Nagel, Kyla A Boyd, Humberto De Vitto, Matthew L Hillestad, Paul G Stalboerger, Matthew T Houdek, Rafael J Sierra, Atta Behfar","doi":"10.1038/s41536-025-00405-2","DOIUrl":"10.1038/s41536-025-00405-2","url":null,"abstract":"<p><p>In the United States, impaired bone healing impacts ~600,000 patients annually. Bone morphogenetic protein 2 (rhBMP2) therapy is impeded by low bone quality and adverse effects. Here, mesenchymal stem cells, engineered to produce BMP2 and BMP2/7 containing extracellular vesicles (BMP2-EV and BMP2/7-EV), provided an alternative means of stimulating bone formation. BMP2-EV and BMP2/7-EV drove increased calcium deposition and alkaline phosphatase activity; with increase in osterix, RUNX2, osteocalcin, and osteopontin documenting osteoblast differentiation. BMP2/7-EV induced SMAD phosphorylation and calcium deposition, was inhibited by DMH1, a BMP I receptor inhibitor, demonstrating BMP receptor dependence. BMP2 and BMP7 extracellular vesicle encapsulation was confirmed with preserved potency following treatment with BMP antagonist, Noggin. Application of BMP2/7-EV in a rat calvarial defect model demonstrated enhanced bone formation on micro-computed tomography and histopathologic analysis, equaling rhBMP2. BMP2/7-EV mediated bone formation here highlights EVs as a unique modality for delivery of tailored polyvalent regenerative biotherapies.</p>","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"26"},"PeriodicalIF":6.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12134205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Binxu Yin, Changhao Yu, Yang Liu, Hao Cai, Wencheng Wu, Tingting Ye, Lei Wang, Lujia Xiao, Yi Zhu, Huaijuan Guo, Kun Zhang, Heng Wang
{"title":"Genetic compensation response contributes to Pleurodeles waltl limb regeneration.","authors":"Binxu Yin, Changhao Yu, Yang Liu, Hao Cai, Wencheng Wu, Tingting Ye, Lei Wang, Lujia Xiao, Yi Zhu, Huaijuan Guo, Kun Zhang, Heng Wang","doi":"10.1038/s41536-025-00417-y","DOIUrl":"10.1038/s41536-025-00417-y","url":null,"abstract":"<p><p>Hippo-Yap/Taz pathway is essential for tissue regeneration in multiple species. However, we found that in the highly regenerative salamanders, Yap knockout does not compromise the limb regeneration due to genetic compensation response (GCR). Specifically, the mutated Yap locus derived non-sense mRNA, which was recognized by UPF3A to instruct compensatory Taz induction. Blocking Yap mRNA or protein indeed inhibits regeneration. GCR could be utilized to maintain the robustness of limb regeneration.</p>","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"25"},"PeriodicalIF":6.4,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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