npj Regenerative Medicine最新文献_第2页

A novel therapy to ameliorate nitrogen mustard-induced limbal stem cell deficiency using lipoprotein-like nanoparticles. 利用脂蛋白样纳米颗粒改善氮芥诱导的角膜缘干细胞缺乏症的新疗法。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-03-20 DOI: 10.1038/s41536-025-00402-5

Elif Kayaalp Nalbant, Timothy J Feliciano, Aliakbar Mohammadlou, Vincent L Xiong, Jacquelyn E Trujillo, Andrea E Calvert, Nihal Kaplan, Parisa Foroozandeh, Jayden Kim, Emma M Bai, Xiaolin Qi, Fernando Tobias, Eric W Roth, Vinayak P Dravid, Kurt Q Lu, SonBinh T Nguyen, C Shad Thaxton, Han Peng, Robert M Lavker

{"title":"A novel therapy to ameliorate nitrogen mustard-induced limbal stem cell deficiency using lipoprotein-like nanoparticles.","authors":"Elif Kayaalp Nalbant, Timothy J Feliciano, Aliakbar Mohammadlou, Vincent L Xiong, Jacquelyn E Trujillo, Andrea E Calvert, Nihal Kaplan, Parisa Foroozandeh, Jayden Kim, Emma M Bai, Xiaolin Qi, Fernando Tobias, Eric W Roth, Vinayak P Dravid, Kurt Q Lu, SonBinh T Nguyen, C Shad Thaxton, Han Peng, Robert M Lavker","doi":"10.1038/s41536-025-00402-5","DOIUrl":"10.1038/s41536-025-00402-5","url":null,"abstract":"Chronic corneal inflammation, a component of sulfur mustard (SM) and nitrogen mustard (NM) injuries frequently leads to limbal stem cell deficiency (LSCD), which can compromise vision. Corneal conjunctivalization, neovascularization, and persistent inflammation are hallmarks of LSCD. Ocular exposure to SM and NM results in an acute and delayed phase of corneal disruption, culminating in LSCD. Available therapies for mustard keratopathy (e.g., topical corticosteroids) often have adverse side effects, and generally are ineffective in preventing the development of LSCD. We developed a novel, optically transparent HDL nanoparticle (NP) with an organic core (oc) molecular scaffold. This unique oc-HDL NP: (i) markedly improved corneal haze during the acute and delayed phases in vivo; (ii) significantly reduced the inflammatory response; and (iii) blunted conjunctivalization and corneal neovascularization during the delayed phase. These findings strongly suggest that our HDL NP is an ideal treatment for mustard keratopathy and other chronic corneal inflammatory diseases.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"14"},"PeriodicalIF":6.4,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune-mediated regeneration of cell-free vascular grafts in an ovine model. 免疫介导的羊无细胞血管移植再生模型。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-03-19 DOI: 10.1038/s41536-025-00400-7

Bita Nasiri, Arundhati Das, Karthik Ramachandran, Sai Harsha Bhamidipati, Yulun Wu, Shriramprasad Venkatesan, Rudiyanto Gunawan, Daniel D Swartz, Stelios T Andreadis

{"title":"Immune-mediated regeneration of cell-free vascular grafts in an ovine model.","authors":"Bita Nasiri, Arundhati Das, Karthik Ramachandran, Sai Harsha Bhamidipati, Yulun Wu, Shriramprasad Venkatesan, Rudiyanto Gunawan, Daniel D Swartz, Stelios T Andreadis","doi":"10.1038/s41536-025-00400-7","DOIUrl":"10.1038/s41536-025-00400-7","url":null,"abstract":"We developed acellular tissue engineered vessels (ATEV) using small intestine submucosa (SIS) incorporating heparin and a novel protein named H2R5. ATEVs were implanted into the arterial circulation of an ovine animal model, demonstrating high primary patency rates over a period of three months. Implanted grafts were infiltrated by host cells, the majority of which were monocytes/macrophages (MC/MΦ), as demonstrated by scRNA sequencing and immunostaining. They also developed functional endothelial and medial layers that deposited new extracellular matrix leading to matrix remodeling and acquisition of mechanical properties that were similar to those of native arteries. Notably, during this short implantation time, ATEVs turned into functional neo-arteries, as evidenced by the development of the vascular contractile function. Our findings underscore the potential of H2R5-functionalized ATEVs as promising candidates for tissue replacement grafts in a large pre-clinical animal model and highlight the contribution of macrophages in vascular regeneration.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"13"},"PeriodicalIF":6.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-scale porosity minimizes foreign body reaction and promotes innervated myofiber formation after volumetric muscle loss. 细胞尺度孔隙减少异物反应和促进神经支配肌纤维形成后，体积肌肉损失。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-03-01 DOI: 10.1038/s41536-025-00395-1

Areli Rodriguez Ayala, George Christ, Donald Griffin

引用次数: 0

Digit regeneration is expedited in LG/J healer mice compared to SM/J non-healer mice. 与SM/J非治疗小鼠相比，LG/J治疗小鼠的手指再生速度加快。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-02-15 DOI: 10.1038/s41536-025-00399-x

Feini Qu, Kristin L Lenz, Gwendalyn L Krekeler, Xin Duan, Muhammad Farooq Rai, Farshid Guilak

{"title":"Digit regeneration is expedited in LG/J healer mice compared to SM/J non-healer mice.","authors":"Feini Qu, Kristin L Lenz, Gwendalyn L Krekeler, Xin Duan, Muhammad Farooq Rai, Farshid Guilak","doi":"10.1038/s41536-025-00399-x","DOIUrl":"10.1038/s41536-025-00399-x","url":null,"abstract":"Limb loss resulting from disease or trauma affects an estimated 185,000 Americans annually, significantly reducing their quality of life. Consequently, successful attempts to regrow missing appendages could substantially improve the prognosis for amputees. In mice, the digit tip spontaneously regenerates resected tissues following distal amputation, whereas this capacity diminishes at more proximal levels after amputation. Moreover, regenerative potential is influenced by genetic variations among inbred mouse strains: LG/J (healer) mice exhibit superior reparative potential compared to SM/J (non-healer) mice. This study investigated the response to various levels of digit amputation in these mice to determine whether this strain-dependent healing response translates to the regeneration of complex tissues. Evaluation of skeletal regrowth, cell proliferation, and differential gene and protein expression reveals that digit regeneration is more robust in LG/J mice compared to SM/J mice at multiple amputation levels, suggesting that the regenerative capacity of composite tissues is genetically heritable in mice.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"11"},"PeriodicalIF":6.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11828864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in bioengineering for descemet membrane endothelial keratoplasty (DMEK). 内皮角膜移植术的生物工程研究进展。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-02-14 DOI: 10.1038/s41536-025-00396-0

Sarah Barbara Zwingelberg, Gizem Karabiyik, Paul Gehle, Melanie von Brandenstein, Sabina Eibichova, Christian Lotz, Florian Groeber-Becker, Daniel Kampik, Ula Jurkunas, Gerd Geerling, Gregor Lang

引用次数: 0

Effects of injury size on local and systemic immune cell dynamics in volumetric muscle loss. 损伤大小对体积性肌肉损失中局部和全身免疫细胞动力学的影响。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-02-13 DOI: 10.1038/s41536-025-00397-z

Ricardo Whitaker, Samuel Sung, Tina Tylek, Gregory E Risser, Erin M O'Brien, Phoebe Ellin Chua, Thomas Li, Ryan J Petrie, Lin Han, Benjamin I Binder-Markey, Kara L Spiller

{"title":"Effects of injury size on local and systemic immune cell dynamics in volumetric muscle loss.","authors":"Ricardo Whitaker, Samuel Sung, Tina Tylek, Gregory E Risser, Erin M O'Brien, Phoebe Ellin Chua, Thomas Li, Ryan J Petrie, Lin Han, Benjamin I Binder-Markey, Kara L Spiller","doi":"10.1038/s41536-025-00397-z","DOIUrl":"10.1038/s41536-025-00397-z","url":null,"abstract":"We took a systems approach to the analysis of macrophage phenotype in regenerative and fibrotic volumetric muscle loss outcomes in mice together with analysis of systemic inflammation and of other leukocytes in the muscle, spleen, and bone marrow. Differences in expression of macrophage phenotype markers occurred as early as day 1, persisted to at least day 28, and were associated with increased numbers of leukocytes in the muscle and bone marrow, increased pro-inflammatory marker expression in splenic macrophages, and changes in the levels of pro-inflammatory cytokines in the blood. The most prominent differences were in muscle neutrophils, which were much more abundant in fibrotic outcomes compared to regenerative outcomes at day 1 after injury. However, neutrophil depletion had little to no effect on macrophage phenotype or on muscle repair outcomes. Together, these results suggest that the entire system of immune cell interactions must be considered to improve muscle repair outcomes.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"9"},"PeriodicalIF":6.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic profiling of iPSC and tissue-derived MSC secretomes reveal a global signature of inflammatory licensing. iPSC和组织来源的MSC分泌组的蛋白质组学分析揭示了炎症许可的全球特征。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-02-04 DOI: 10.1038/s41536-024-00382-y

Margeaux Hodgson-Garms, Matthew J Moore, Mikaël M Martino, Kilian Kelly, Jessica E Frith

{"title":"Proteomic profiling of iPSC and tissue-derived MSC secretomes reveal a global signature of inflammatory licensing.","authors":"Margeaux Hodgson-Garms, Matthew J Moore, Mikaël M Martino, Kilian Kelly, Jessica E Frith","doi":"10.1038/s41536-024-00382-y","DOIUrl":"10.1038/s41536-024-00382-y","url":null,"abstract":"Much of the therapeutic potential of mesenchymal stromal cells (MSCs) is underpinned by their secretome which varies significantly with source, donor and microenvironmental cues. Understanding these differences is essential to define the mechanisms of MSC-based tissue repair and optimise cell therapies. This study analysed the secretomes of bone-marrow (BM.MSCs), umbilical-cord (UC.MSCs), adipose-tissue (AT.MSCs) and clinical/commercial-grade induced pluripotent stem cell-derived MSCs (iMSCs), under resting and inflammatory licenced conditions. iMSCs recapitulated the inflammatory licensing process, validating their comparability to tissue-derived MSCs. Overall, resting secretomes were defined by extracellular matrix (ECM) and pro-regenerative proteins, while licensed secretomes were enriched in chemotactic and immunomodulatory proteins. iMSC and UC.MSC secretomes contained proteins indicating proliferative potential and telomere maintenance, whereas adult tissue-derived secretomes contained fibrotic and ECM-related proteins. The data and findings from this study will inform the optimum MSC source for particular applications and underpin further development of MSC therapies.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"7"},"PeriodicalIF":6.4,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11794695/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-orthogonal crosslinking and hyaluronan facilitate transparent healing after treatment of deep corneal injuries with in situ-forming hydrogels. 生物正交交联和透明质酸促进透明愈合后治疗深层角膜损伤的位置形成的水凝胶。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-02-04 DOI: 10.1038/s41536-024-00385-9

Fang Chen, Uiyoung Han, Thitima Wungcharoen, Youngyoon Amy Seo, Peter Le, Li Jiang, Nae-Won Kang, Euisun Song, Kyeongwoo Jang, David Mundy, Gabriella Maria Fernandes-Cunha, Sarah Heilshorn, David Myung

{"title":"Bio-orthogonal crosslinking and hyaluronan facilitate transparent healing after treatment of deep corneal injuries with in situ-forming hydrogels.","authors":"Fang Chen, Uiyoung Han, Thitima Wungcharoen, Youngyoon Amy Seo, Peter Le, Li Jiang, Nae-Won Kang, Euisun Song, Kyeongwoo Jang, David Mundy, Gabriella Maria Fernandes-Cunha, Sarah Heilshorn, David Myung","doi":"10.1038/s41536-024-00385-9","DOIUrl":"10.1038/s41536-024-00385-9","url":null,"abstract":"Corneal transplantation is the primary treatment for corneal blindness, affecting millions globally. However, challenges like donor scarcity and surgical complications remain. Recently, in situ-forming corneal stroma substitutes have emerged, offering potential solutions to these limitations. These substitutes enable liquid-to-hydrogel formation in situ, eliminating sutures and reducing complications. Here we performed a direct, side-by-side comparison of a composite hyaluronan-collagen (HA-Col) hydrogel crosslinked by either photochemistry or bio-orthogonal chemistry to ascertain the impact of reaction specificity on corneal wound healing. Testing in rodent and rabbit models suggests that composite HA-Col gels crosslinked by bio-orthogonal chemistry results in more rapid and optically favorable wound healing compared to the same composition crosslinked by photochemistry as well as bio-orthogonally crosslinked collagen alone. These findings underscore biochemical parameters that may be important to the success of crosslinked, in situ-forming hydrogels as an alternative to corneal transplantation, with the potential for expanded access to treatment and improved outcomes.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"8"},"PeriodicalIF":6.4,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11794660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryopreserved human alternatively activated macrophages promote resolution of acetaminophen-induced liver injury in mouse. 低温保存的人选择性活化巨噬细胞促进对乙酰氨基酚诱导的小鼠肝损伤的消退。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-01-22 DOI: 10.1038/s41536-025-00393-3

Maria Elena Candela, Melisande Addison, Rhona Aird, Tak-Yung Man, Jennifer A Cartwright, Candice Ashmore-Harris, Alastair M Kilpatrick, Philip J Starkey Lewis, Anna Drape, Mark Barnett, Donna Mitchell, Colin McLean, Neil McGowan, Marc Turner, James W Dear, Stuart J Forbes

{"title":"Cryopreserved human alternatively activated macrophages promote resolution of acetaminophen-induced liver injury in mouse.","authors":"Maria Elena Candela, Melisande Addison, Rhona Aird, Tak-Yung Man, Jennifer A Cartwright, Candice Ashmore-Harris, Alastair M Kilpatrick, Philip J Starkey Lewis, Anna Drape, Mark Barnett, Donna Mitchell, Colin McLean, Neil McGowan, Marc Turner, James W Dear, Stuart J Forbes","doi":"10.1038/s41536-025-00393-3","DOIUrl":"10.1038/s41536-025-00393-3","url":null,"abstract":"Acute liver failure is a rapidly progressing, life-threatening condition most commonly caused by an overdose of acetaminophen (paracetamol). The antidote, N-acetylcysteine (NAC), has limited efficacy when liver injury is established. If acute liver damage is severe, liver failure can rapidly develop with associated high mortality rates. We have previously demonstrated that alternatively, activated macrophages are a potential therapeutic option to reverse acute liver injury in pre-clinical models. In this paper, we present data using cryopreserved human alternatively activated macrophages (hAAMs)-which represent a potential, rapidly available treatment suitable for use in the acute setting. In a mouse model of APAP-induced injury, peripherally injected cryopreserved hAAMs reduced liver necrosis, modulated inflammatory responses, and enhanced liver regeneration. hAAMs were effective even when administered after the therapeutic window for NAC. This cell therapy approach represents a potential treatment for APAP overdose when NAC is ineffective because liver injury is established.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"5"},"PeriodicalIF":6.4,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revitalizing the heart: strategies and tools for cardiomyocyte regeneration post-myocardial infarction. 振兴心脏：心肌梗死后心肌细胞再生的策略和工具。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-01-22 DOI: 10.1038/s41536-025-00394-2

Axelle Bois, Catarina Grandela, James Gallant, Christine Mummery, Philippe Menasché

引用次数: 0