npj Regenerative Medicine最新文献_第10页

Combinatorial extracellular matrix tissue chips for optimizing mesenchymal stromal cell microenvironment and manufacturing. 优化间充质间质细胞微环境及制造的组合细胞外基质组织芯片。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-04-22 DOI: 10.1038/s41536-025-00408-z

Ishita Jain, Alex H P Chan, Guang Yang, Hao He, Johnny Lam, Kyung Sung, Ngan F Huang

{"title":"Combinatorial extracellular matrix tissue chips for optimizing mesenchymal stromal cell microenvironment and manufacturing.","authors":"Ishita Jain, Alex H P Chan, Guang Yang, Hao He, Johnny Lam, Kyung Sung, Ngan F Huang","doi":"10.1038/s41536-025-00408-z","DOIUrl":"10.1038/s41536-025-00408-z","url":null,"abstract":"Despite the therapeutic potential of mesenchymal stromal cells (MSC), there is limited understanding of optimal extracellular matrix (ECM) environments to manufacture these cells. We developed tissue chips to study the effects of multi-factorial ECM environments under manufacturable stiffness ranges and multi-component ECM compositions. Manufacturing qualities of cell expansion potential, immunomodulation, and differentiation capacity were examined. The results show stiffness effects, with 900 kPa substrates supporting higher proliferation and osteogenic differentiation, along with anti-inflammatory IL-10 expression, whereas 150 kPa substrates promoted adipogenic differentiation at 150 kPa, suggesting that optimal ECM environments may differ based on manufacturing goals. ECM biochemistries containing fibronectin and laminin further modulated MSC manufacturing qualities across various stiffnesses. Proteomic and transcriptomic analyses revealed unique ECM combinations that induced higher levels of angiogenic and immunomodulatory cytokines, compared to single factor ECMs. These findings demonstrate that optimized ECM environments enhance MSC manufacturing quality.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"21"},"PeriodicalIF":6.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144057914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allogeneic RPE cell suspension manufactured at scale demonstrating preclinical safety and efficacy led to IND approval. 大规模生产的同种异体RPE细胞悬浮液证明了临床前安全性和有效性，并获得了IND批准。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-04-19 DOI: 10.1038/s41536-025-00407-0

Lalitha Soundararajan, Harshini Surendran, Niharika Patlolla, Rajani Battu, Jonathan Stoddard, Sebastian Arrizabalaga, Zengping Liu, Gopal Lingam, Xinyi Su, Renee C Ryals, Rajarshi Pal

{"title":"Allogeneic RPE cell suspension manufactured at scale demonstrating preclinical safety and efficacy led to IND approval.","authors":"Lalitha Soundararajan, Harshini Surendran, Niharika Patlolla, Rajani Battu, Jonathan Stoddard, Sebastian Arrizabalaga, Zengping Liu, Gopal Lingam, Xinyi Su, Renee C Ryals, Rajarshi Pal","doi":"10.1038/s41536-025-00407-0","DOIUrl":"https://doi.org/10.1038/s41536-025-00407-0","url":null,"abstract":"Cell replacement therapy is a promising therapeutic option for dry age-related macular degeneration (AMD). In this study, we outline our design for scalable manufacture with appropriate quality gates and present in vivo data for establishing preclinical safety and efficacy of an induced pluripotent stem cell (iPSC)-derived retinal pigment epithelium (RPE) product, thus laying the foundation for Phase 1/2a trial approval in India (ClinicalTrials.gov ID: NCT06394232; date of registration: 23rd September 2024). Escalating doses of RPE cell suspension in immunocompromised animals demonstrated absence of tumor formation up to 9 months post-injection. Good Laboratory Practices (GLP) toxicology and tolerability studies in rabbits and non-human primates (NHP) respectively showed no major adverse events. RPE transplanted into immune suppressed RCS rats showed integration, neuroprotection and rescue of visual function. In addition, we provide a detailed description of the modifications in GMP manufacturing protocol to create a final product with a unique composition and Chemistry, Manufacturing and Controls (CMC) studies performed during product development.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"19"},"PeriodicalIF":6.4,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12009284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enabling adenosine signaling to promote aged fracture healing. 激活腺苷信号促进老年骨折愈合。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-04-09 DOI: 10.1038/s41536-025-00406-1

Hunter Newman, Yu-Ru V Shih, Jiaul Hoque, Yuze Zeng, Naveen R Natesh, Gavin Gonzales, Wendi Guo, Vijitha Puviindran, Colleen Wu, Benjamin A Alman, Shyni Varghese

{"title":"Enabling adenosine signaling to promote aged fracture healing.","authors":"Hunter Newman, Yu-Ru V Shih, Jiaul Hoque, Yuze Zeng, Naveen R Natesh, Gavin Gonzales, Wendi Guo, Vijitha Puviindran, Colleen Wu, Benjamin A Alman, Shyni Varghese","doi":"10.1038/s41536-025-00406-1","DOIUrl":"10.1038/s41536-025-00406-1","url":null,"abstract":"Bone fractures and related complications are a significant concern for older adults, particularly with the growing aging population. Therapeutic interventions that promote bone tissue regeneration are attractive for geriatric fracture repair. Extracellular adenosine plays a key role in bone homeostasis and regeneration. Herein, we examined the changes in extracellular adenosine with aging and the potential of local delivery of adenosine to promote fracture healing using aged mice. Extracellular adenosine level was found to be significantly lower in aged bone tissue compared to young mice. Concomitantly, the ecto-5'-nucleotidase CD73 expression was also lower in aged bone. Local delivery of adenosine using injectable, in situ curing microgel delivery units yielded a pro-regenerative environment and promoted fracture healing in aged mice. This study offers new insights into age-related physiological changes in adenosine levels and demonstrates the therapeutic potential of adenosine supplementation to circumvent the compromised healing of geriatric fractures.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"18"},"PeriodicalIF":6.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982386/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preclinical efficacy and safety of AAVrh10-based plakophilin-2 gene therapy (LX2020) as a treatment for arrhythmogenic cardiomyopathy. 基于aavrh10的plakophilin-2基因疗法（LX2020）治疗心律失常性心肌病的临床前疗效和安全性

IF 6.5 1区医学

npj Regenerative Medicine Pub Date : 2025-04-03 DOI: 10.1038/s41536-025-00401-6

Jing Zhang, Erika Joana Gutierrez-Lara, Aryanne Do, Lena Nguyen, Anju Nair, Nithya Selvan, Tim Fenn, Eric Adler, Richie Khanna, Farah Sheikh

{"title":"Preclinical efficacy and safety of AAVrh10-based plakophilin-2 gene therapy (LX2020) as a treatment for arrhythmogenic cardiomyopathy.","authors":"Jing Zhang, Erika Joana Gutierrez-Lara, Aryanne Do, Lena Nguyen, Anju Nair, Nithya Selvan, Tim Fenn, Eric Adler, Richie Khanna, Farah Sheikh","doi":"10.1038/s41536-025-00401-6","DOIUrl":"10.1038/s41536-025-00401-6","url":null,"abstract":"Plakophilin-2 (PKP2) mutations cause fatal genetic heart disease and arrhythmogenic cardiomyopathy (ACM) with primary effects on the right ventricle (RV). Adeno-associated virus (AAV)-PKP2 gene therapy shows promise as a therapeutic strategy but lacks long-term data and guidelines on minimal effective doses in animal studies for treating RV deficits, arrhythmia burden, and improving survival when administered during disease settings, which are most relevant to clinical trials. Using AAVrh10, known for its preferential cardiac gene expression at lower doses, we show minimal doses required for efficacy for AAVrh10.PKP2 (LX2020) to rescue cardiac (molecular and especially RV) deficits, arrhythmia burden and survival in PKP2 ACM mice, suggesting its potential to reverse late-stage pathology. Safety assessments in non-human primates revealed no adverse events. These data support LX2020 as a viable treatment for PKP2 ACM patients.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"17"},"PeriodicalIF":6.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11965304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143774792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fibroblast-derived osteoglycin promotes epithelial cell repair. 成纤维细胞来源的骨胰素促进上皮细胞修复。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-03-25 DOI: 10.1038/s41536-025-00404-3

Luke van der Koog, Manon E Woest, Iris C Gorter, Vicky Verschut, Robin A B Elferink, Annet B Zuidhof, Dyan F Nugraha, Maunick L Koloko Ngassie, Sophie I T Bos, Deepesh Dhakad, Justina C Wolters, Peter L Horvatovich, Y S Prakash, Wim Timens, Önder A Yildirim, Corry-Anke Brandsma, Henderik W Frijlink, Anika Nagelkerke, Reinoud Gosens

{"title":"Fibroblast-derived osteoglycin promotes epithelial cell repair.","authors":"Luke van der Koog, Manon E Woest, Iris C Gorter, Vicky Verschut, Robin A B Elferink, Annet B Zuidhof, Dyan F Nugraha, Maunick L Koloko Ngassie, Sophie I T Bos, Deepesh Dhakad, Justina C Wolters, Peter L Horvatovich, Y S Prakash, Wim Timens, Önder A Yildirim, Corry-Anke Brandsma, Henderik W Frijlink, Anika Nagelkerke, Reinoud Gosens","doi":"10.1038/s41536-025-00404-3","DOIUrl":"10.1038/s41536-025-00404-3","url":null,"abstract":"There is an urgent need for innovative therapies targeting defective epithelial repair in chronic diseases like COPD. The mesenchymal niche is a critical regulator in epithelial stem cell activation, suggesting that their secreted factors are possible potent drug targets. Utilizing a proteomics-guided drug discovery strategy, we explored the lung fibroblast secretome to uncover impactful drug targets. Our lung organoid assays identified several regenerative ligands, with osteoglycin (OGN) showing the most profound effects. Transcriptomic analyses revealed that OGN enhances alveolar progenitor differentiation, detoxifies reactive oxygen species, and strengthens fibroblast-epithelial crosstalk. OGN expression was diminished in COPD patients and smoke-exposed mice. An active fragment of OGN (leucine-rich repeat regions 4-7) replicated full-length OGN's regenerative effects, significantly ameliorating elastase-induced lung injury in lung slices and improving lung function in vivo. These findings highlight OGN as a pivotal secreted protein for alveolar epithelial repair, positioning its active fragment as a promising therapeutic for COPD.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"16"},"PeriodicalIF":6.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantum dots as biocompatible small RNA nanocarriers modulating macrophage polarization to treat Asherman's syndrome. 量子点作为生物相容性小RNA纳米载体调节巨噬细胞极化治疗阿什曼综合征。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-03-25 DOI: 10.1038/s41536-025-00403-4

Ji Eun Won, Mira Park, Seok-Ho Hong, Yeon Sun Kim, Haengseok Song

{"title":"Quantum dots as biocompatible small RNA nanocarriers modulating macrophage polarization to treat Asherman's syndrome.","authors":"Ji Eun Won, Mira Park, Seok-Ho Hong, Yeon Sun Kim, Haengseok Song","doi":"10.1038/s41536-025-00403-4","DOIUrl":"10.1038/s41536-025-00403-4","url":null,"abstract":"Macrophages play a key role in host defense and inflammation, with polarization ranging from pro-inflammatory M1 to anti-inflammatory M2 states. However, effective modulation of macrophage polarity via nucleotide delivery is challenging. This study developed polyethyleneimine-modified carboxyl quantum dots (QDP) as a biocompatible carrier for small RNA delivery to modulate macrophage polarization. QDP-mediated delivery of miR-10a (QDP/miR-10a) rebalanced macrophage polarity and alleviated uterine inflammation and fibrosis in a mouse model of Asherman's syndrome (AS). In vitro, QDP effectively delivered small RNA into RAW 264.7 cells without cytotoxicity, converting LPS-induced M1 to M2 macrophages by inhibiting NF-κB, MAPK, and AKT signaling. In vivo, QDP/miR-10a reduced M1 macrophages, restored polarization, and enhanced uterine restoration in AS mice without affecting systemic immunity. Thus, QDP represents a safe and effective nanocarrier for small RNA delivery to modulate macrophage polarization for inflammatory disease treatment, including AS.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"15"},"PeriodicalIF":6.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel therapy to ameliorate nitrogen mustard-induced limbal stem cell deficiency using lipoprotein-like nanoparticles. 利用脂蛋白样纳米颗粒改善氮芥诱导的角膜缘干细胞缺乏症的新疗法。

IF 7 1区医学

npj Regenerative Medicine Pub Date : 2025-03-20 DOI: 10.1038/s41536-025-00402-5

Elif Kayaalp Nalbant, Timothy J Feliciano, Aliakbar Mohammadlou, Vincent L Xiong, Jacquelyn E Trujillo, Andrea E Calvert, Nihal Kaplan, Parisa Foroozandeh, Jayden Kim, Emma M Bai, Xiaolin Qi, Fernando Tobias, Eric W Roth, Vinayak P Dravid, Kurt Q Lu, SonBinh T Nguyen, C Shad Thaxton, Han Peng, Robert M Lavker

{"title":"A novel therapy to ameliorate nitrogen mustard-induced limbal stem cell deficiency using lipoprotein-like nanoparticles.","authors":"Elif Kayaalp Nalbant, Timothy J Feliciano, Aliakbar Mohammadlou, Vincent L Xiong, Jacquelyn E Trujillo, Andrea E Calvert, Nihal Kaplan, Parisa Foroozandeh, Jayden Kim, Emma M Bai, Xiaolin Qi, Fernando Tobias, Eric W Roth, Vinayak P Dravid, Kurt Q Lu, SonBinh T Nguyen, C Shad Thaxton, Han Peng, Robert M Lavker","doi":"10.1038/s41536-025-00402-5","DOIUrl":"10.1038/s41536-025-00402-5","url":null,"abstract":"Chronic corneal inflammation, a component of sulfur mustard (SM) and nitrogen mustard (NM) injuries frequently leads to limbal stem cell deficiency (LSCD), which can compromise vision. Corneal conjunctivalization, neovascularization, and persistent inflammation are hallmarks of LSCD. Ocular exposure to SM and NM results in an acute and delayed phase of corneal disruption, culminating in LSCD. Available therapies for mustard keratopathy (e.g., topical corticosteroids) often have adverse side effects, and generally are ineffective in preventing the development of LSCD. We developed a novel, optically transparent HDL nanoparticle (NP) with an organic core (oc) molecular scaffold. This unique oc-HDL NP: (i) markedly improved corneal haze during the acute and delayed phases in vivo; (ii) significantly reduced the inflammatory response; and (iii) blunted conjunctivalization and corneal neovascularization during the delayed phase. These findings strongly suggest that our HDL NP is an ideal treatment for mustard keratopathy and other chronic corneal inflammatory diseases.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"14"},"PeriodicalIF":7.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune-mediated regeneration of cell-free vascular grafts in an ovine model. 免疫介导的羊无细胞血管移植再生模型。

IF 7 1区医学

npj Regenerative Medicine Pub Date : 2025-03-19 DOI: 10.1038/s41536-025-00400-7

Bita Nasiri, Arundhati Das, Karthik Ramachandran, Sai Harsha Bhamidipati, Yulun Wu, Shriramprasad Venkatesan, Rudiyanto Gunawan, Daniel D Swartz, Stelios T Andreadis

{"title":"Immune-mediated regeneration of cell-free vascular grafts in an ovine model.","authors":"Bita Nasiri, Arundhati Das, Karthik Ramachandran, Sai Harsha Bhamidipati, Yulun Wu, Shriramprasad Venkatesan, Rudiyanto Gunawan, Daniel D Swartz, Stelios T Andreadis","doi":"10.1038/s41536-025-00400-7","DOIUrl":"10.1038/s41536-025-00400-7","url":null,"abstract":"We developed acellular tissue engineered vessels (ATEV) using small intestine submucosa (SIS) incorporating heparin and a novel protein named H2R5. ATEVs were implanted into the arterial circulation of an ovine animal model, demonstrating high primary patency rates over a period of three months. Implanted grafts were infiltrated by host cells, the majority of which were monocytes/macrophages (MC/MΦ), as demonstrated by scRNA sequencing and immunostaining. They also developed functional endothelial and medial layers that deposited new extracellular matrix leading to matrix remodeling and acquisition of mechanical properties that were similar to those of native arteries. Notably, during this short implantation time, ATEVs turned into functional neo-arteries, as evidenced by the development of the vascular contractile function. Our findings underscore the potential of H2R5-functionalized ATEVs as promising candidates for tissue replacement grafts in a large pre-clinical animal model and highlight the contribution of macrophages in vascular regeneration.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"13"},"PeriodicalIF":7.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-scale porosity minimizes foreign body reaction and promotes innervated myofiber formation after volumetric muscle loss. 细胞尺度孔隙减少异物反应和促进神经支配肌纤维形成后，体积肌肉损失。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-03-01 DOI: 10.1038/s41536-025-00395-1

Areli Rodriguez Ayala, George Christ, Donald Griffin

引用次数: 0

Digit regeneration is expedited in LG/J healer mice compared to SM/J non-healer mice. 与SM/J非治疗小鼠相比，LG/J治疗小鼠的手指再生速度加快。

IF 6.4 1区医学

npj Regenerative Medicine Pub Date : 2025-02-15 DOI: 10.1038/s41536-025-00399-x

Feini Qu, Kristin L Lenz, Gwendalyn L Krekeler, Xin Duan, Muhammad Farooq Rai, Farshid Guilak

{"title":"Digit regeneration is expedited in LG/J healer mice compared to SM/J non-healer mice.","authors":"Feini Qu, Kristin L Lenz, Gwendalyn L Krekeler, Xin Duan, Muhammad Farooq Rai, Farshid Guilak","doi":"10.1038/s41536-025-00399-x","DOIUrl":"10.1038/s41536-025-00399-x","url":null,"abstract":"Limb loss resulting from disease or trauma affects an estimated 185,000 Americans annually, significantly reducing their quality of life. Consequently, successful attempts to regrow missing appendages could substantially improve the prognosis for amputees. In mice, the digit tip spontaneously regenerates resected tissues following distal amputation, whereas this capacity diminishes at more proximal levels after amputation. Moreover, regenerative potential is influenced by genetic variations among inbred mouse strains: LG/J (healer) mice exhibit superior reparative potential compared to SM/J (non-healer) mice. This study investigated the response to various levels of digit amputation in these mice to determine whether this strain-dependent healing response translates to the regeneration of complex tissues. Evaluation of skeletal regrowth, cell proliferation, and differential gene and protein expression reveals that digit regeneration is more robust in LG/J mice compared to SM/J mice at multiple amputation levels, suggesting that the regenerative capacity of composite tissues is genetically heritable in mice.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":"10 1","pages":"11"},"PeriodicalIF":6.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11828864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0