Functional ProTracer identifies patterns of cell proliferation in tissues and underlying regulatory mechanisms.

IF 6.4 1区医学 Q1 CELL & TISSUE ENGINEERING

npj Regenerative Medicine Pub Date : 2023-08-03 DOI:10.1038/s41536-023-00318-y

Xiuxiu Liu, Maoying Han, Wendong Weng, Yan Li, Wenjuan Pu, Kuo Liu, Xufeng Li, Lingjuan He, Ruilin Sun, Ruling Shen, Yulong He, Dandan Liang, Yi-Han Chen, Qing-Dong Wang, Jan S Tchorz, Bin Zhou

引用次数: 0

Abstract

A genetic system, ProTracer, has been recently developed to record cell proliferation in vivo. However, the ProTracer is initiated by an infrequently used recombinase Dre, which limits its broad application for functional studies employing floxed gene alleles. Here we generated Cre-activated functional ProTracer (fProTracer) mice, which enable simultaneous recording of cell proliferation and tissue-specific gene deletion, facilitating broad functional analysis of cell proliferation by any Cre driver.

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功能ProTracer识别组织中细胞增殖的模式和潜在的调节机制。

一种名为ProTracer的遗传系统最近被开发出来用于记录细胞在体内的增殖。然而，ProTracer是由一种不常用的重组酶Dre启动的，这限制了它在使用固定基因等位基因的功能研究中的广泛应用。在这里，我们生成了Cre激活的功能性ProTracer (fProTracer)小鼠，它可以同时记录细胞增殖和组织特异性基因缺失，从而促进了通过任何Cre驱动程序对细胞增殖进行广泛的功能分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

npj Regenerative Medicine Engineering-Biomedical Engineering

CiteScore

10.00

自引率

1.40%

发文量

审稿时长

12 weeks

期刊介绍： Regenerative Medicine, an innovative online-only journal, aims to advance research in the field of repairing and regenerating damaged tissues and organs within the human body. As a part of the prestigious Nature Partner Journals series and in partnership with ARMI, this high-quality, open access journal serves as a platform for scientists to explore effective therapies that harness the body's natural regenerative capabilities. With a focus on understanding the fundamental mechanisms of tissue damage and regeneration, npj Regenerative Medicine actively encourages studies that bridge the gap between basic research and clinical tissue repair strategies.