npj Regenerative Medicine最新文献_第4页

A characterization of axolotl digit regeneration: conserved mechanisms, divergent patterning, and a critical role for hedgehog signaling. 蝾螈手指再生的特征：保守机制，分化模式和刺猬信号的关键作用。

IF 7 1区医学

npj Regenerative Medicine Pub Date : 2026-03-25 DOI: 10.1038/s41536-026-00461-2

Jackson R Griffiths, Melissa Miller, Timothy J Duerr, Ashlin E Owen, James R Monaghan

{"title":"A characterization of axolotl digit regeneration: conserved mechanisms, divergent patterning, and a critical role for hedgehog signaling.","authors":"Jackson R Griffiths, Melissa Miller, Timothy J Duerr, Ashlin E Owen, James R Monaghan","doi":"10.1038/s41536-026-00461-2","DOIUrl":"10.1038/s41536-026-00461-2","url":null,"abstract":"Axolotl digits offer an experimentally versatile model for studying complex tissue regeneration. Here, we provide a comprehensive morphological and molecular characterization of digit regeneration, revealing both conserved features and notable divergences from classical limb regeneration. Digit blastemas progress through similar morphological stages, are nerve-dependent, contain key regenerative cell populations, and express many canonical morphogens and mitogens. However, they exhibit minimal expression of the A-P patterning genes Shh, Fgf8, and Grem1; suggesting distal outgrowth and patterning occur independently of these signals. Joint regenerative fidelity varies significantly across digits and cannot be explained by differences in nerve supply, cell proliferation, or differential expression of any patterning genes assessed in this study. Furthermore, functional experiments reveal Hedgehog signaling is essential for interphalangeal joint regeneration, but activation alone is insufficient to improve fidelity in less robust digits. This system combines experimental accessibility with intrinsic variation in regenerative outcomes, making it an ideal platform to identify critical determinants of successful tissue regeneration and refine models of appendage patterning.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":" ","pages":""},"PeriodicalIF":7.0,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13250136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147516599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wnt-presenting materials sustain H3K14-acetylation in human skeletal stem cells for tissue engineering and bone repair. wnt呈递材料在人体骨骼干细胞中维持h3k14 -乙酰化，用于组织工程和骨修复。

IF 7 1区医学

npj Regenerative Medicine Pub Date : 2026-03-17 DOI: 10.1038/s41536-026-00467-w

Pierre Becquart, Pierre Tournier, Sergi Junyent, Binbin Ma, Yu Liu, Yin Tang, Xin Chen, Dong Fang, Anjali P Kusumbe, Shukry J Habib

{"title":"Wnt-presenting materials sustain H3K14-acetylation in human skeletal stem cells for tissue engineering and bone repair.","authors":"Pierre Becquart, Pierre Tournier, Sergi Junyent, Binbin Ma, Yu Liu, Yin Tang, Xin Chen, Dong Fang, Anjali P Kusumbe, Shukry J Habib","doi":"10.1038/s41536-026-00467-w","DOIUrl":"10.1038/s41536-026-00467-w","url":null,"abstract":"Engineering functional tissues for transplantation requires insight into epigenetic mechanisms that regulate stem cell fate. We have developed the Wnt-induced osteogenic tissue model (WIOTM), a platform that recapitulates human osteogenesis, and identified acetylation of histone H3 at lysine 14 (H3K14ac) as a critical epigenetic regulator in human skeletal stem cells (hSSCs). In WIOTM, localized Wnt signals drive asymmetric cell division (ACD), yielding a proximal hSSC with high H3K14ac and a distal daughter with reduced H3K14ac that migrates into the 3D collagen matrix and initiates osteogenic differentiation. Disrupting H3K14ac in hSSCs abrogates ACD and WIOTM formation. To test whether hSSCs maintain H3K14ac in vivo, we formed the WIOTM on Wnt-functionalized polymer bandages and transplanted them into calvarial defects. The WIOTM contributed to bone repair, and human cells adjacent to the bandages retained high H3K14ac despite the injury environment. These findings establish WIOTM as both a mechanistic and translational platform for regenerative medicine.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":" ","pages":""},"PeriodicalIF":7.0,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13250124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147476280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seeing through collagen: integrative pro-regenerative corneal implants for clearer future. 透过胶原蛋白透视：一体化促再生角膜植入，创造更清晰的未来。

IF 7 1区医学

npj Regenerative Medicine Pub Date : 2026-03-14 DOI: 10.1038/s41536-026-00471-0

Xia Huang, Mohammad M Islam, Stephanie L Watson, Hirak K Patra

引用次数: 0

Non-canonical muscle stem cells and other lessons from regenerative vertebrates. 非典型肌肉干细胞和其他再生脊椎动物的经验教训。

IF 7 1区医学

npj Regenerative Medicine Pub Date : 2026-03-10 DOI: 10.1038/s41536-026-00468-9

Vijayishwer Singh Jamwal, Wouter Masselink, Karen Crawford, Prayag Murawala

引用次数: 0

Single-cell chromatin accessibility landscape of cardiac non-myocytes identifies tissue repair program during heart regeneration. 心脏非肌细胞单细胞染色质可及性景观识别心脏再生过程中的组织修复程序。

IF 7 1区医学

npj Regenerative Medicine Pub Date : 2026-03-05 DOI: 10.1038/s41536-026-00465-y

Zihao Chen, Yage Nie, Liying Huang, Ni Zeng, Jixing Gong, Yichen Gao, Qiye Wen, Xiaoyong Chen, Xiaoqian Ji, Yun Li, Tian Lan, Lan Jiang, Jia Wang, Jin Xu, Nan Cao

{"title":"Single-cell chromatin accessibility landscape of cardiac non-myocytes identifies tissue repair program during heart regeneration.","authors":"Zihao Chen, Yage Nie, Liying Huang, Ni Zeng, Jixing Gong, Yichen Gao, Qiye Wen, Xiaoyong Chen, Xiaoqian Ji, Yun Li, Tian Lan, Lan Jiang, Jia Wang, Jin Xu, Nan Cao","doi":"10.1038/s41536-026-00465-y","DOIUrl":"10.1038/s41536-026-00465-y","url":null,"abstract":"The restricted regenerative potential of adult hearts poses a significant barrier to effective repair following injury. In contrast to numerous vertebrates, mammalian hearts exhibit only transient neonatal renewal capacity during the initial days of life. Beyond cardiomyocytes, understanding the diverse compositions of non-cardiomyocytes (non-CMs) is imperative for maintaining heart microenvironment homeostasis during neonatal heart regeneration. Here, we conduct single-cell ATAC sequencing on neonatal hearts at varying time points post-apical resection to profile the epigenetic landscape. Intriguingly, fibroblasts and endothelial cells, as the most abundant populations in the heart, exhibit the most dynamic chromatin remodeling upon injury. Furthermore, we reveal CEBPD and AP-1 family transcriptional factors as pivotal trans-regulators orchestrating these alterations, governing beneficial fibroblast activation and endothelial cell angiogenesis crucial for cardiac regeneration, respectively. Collectively, our study delineates the cellular identity of non-CMs at the epigenome level using single-cell approaches, offering insights into cell type-targeted interventions for heart regeneration.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":" ","pages":""},"PeriodicalIF":7.0,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13076798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147365965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunomodulatory and anabolic biphasic scaffold with hierarchical biomimetic structure directed osteochondral defect repair. 免疫调节与合成代谢双相支架的分级仿生结构定向骨软骨缺损修复。

IF 7 1区医学

npj Regenerative Medicine Pub Date : 2026-03-03 DOI: 10.1038/s41536-026-00463-0

Hao Yu, Wei Wang, Hongning Wang, Wenyi Zhang, Yan Zheng, Luya Chen, Shenbin Huang, Wentao Yan, Qingqing Yao

{"title":"Immunomodulatory and anabolic biphasic scaffold with hierarchical biomimetic structure directed osteochondral defect repair.","authors":"Hao Yu, Wei Wang, Hongning Wang, Wenyi Zhang, Yan Zheng, Luya Chen, Shenbin Huang, Wentao Yan, Qingqing Yao","doi":"10.1038/s41536-026-00463-0","DOIUrl":"10.1038/s41536-026-00463-0","url":null,"abstract":"Repairing osteochondral tissue is challenging due to its hierarchical structure, mechanical heterogeneity, and the need for spatial control over stem cell differentiation. Advances in tissue engineering have facilitated the development of biphasic cartilage-bone integrated scaffolds for osteochondral repair. The cartilage layer, composed of an IGF-1-loaded polydopamine-ZIF8/HAMA hydrogel, mimicked native tissue and enabled controlled cytokine release. This layer promoted M2 macrophage polarization, enhanced BMSC migration and chondrogenesis, and improved cartilage anabolism. The subchondral bone layer was a nanoclay (XLS)-functionalized 3D bioglass scaffold, which provided superior mechanical strength and supported osteogenic differentiation. These layers were integrated via partial interpenetration of the HAMA hydrogel. Importantly, in vivo studies confirmed that our biphasic scaffold effectively promoted osteochondral defects regeneration in a rat lower femoral osteochondral defect model. Collectively, this biphasic scaffold system presents a promising therapeutic strategy for osteochondral tissue regeneration.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":" ","pages":""},"PeriodicalIF":7.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13066413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147349865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel approach for diabetic wound healing: adipose-derived mesenchymal stromal cells Exo@SPHydrogel combined with laser therapy. 糖尿病伤口愈合的新方法：脂肪源性间充质细胞Exo@SPHydrogel联合激光治疗。

IF 7 1区医学

npj Regenerative Medicine Pub Date : 2026-02-18 DOI: 10.1038/s41536-026-00459-w

Weizhi Chen, Jie Hong, Yiliu Wei, Junchen Ye, Qiaoyi Wu

引用次数: 0

Telomere shortening in laminopathic dilated cardiomyopathy. 层压型扩张型心肌病的端粒缩短。

IF 7 1区医学

npj Regenerative Medicine Pub Date : 2026-02-10 DOI: 10.1038/s41536-026-00462-1

Alex C Y Chang, Gaspard Pardon, Andrew C H Chang, Chenguang Wang, Vittavat Termglinchan, Anna Kirillova, Luka Nicin, Foster Birnbaum, Annie Laquerrière, Gisele Bonne, Joseph Wu, Helen M Blau

引用次数: 0

Novel copper-ion coordinated andrographolide-loaded hydrogel activates Rac1/JNK1 axis for enhancing diabetic wound healing. 新型铜离子协同穿心术内酯负载水凝胶激活Rac1/JNK1轴促进糖尿病伤口愈合。

IF 7 1区医学

npj Regenerative Medicine Pub Date : 2026-02-06 DOI: 10.1038/s41536-026-00457-y

Penghui Ye, Yuhe Dai, Qianbo Zhang, Junqi Yang, Lele Liu, Xiuying Guo, Huan Zhu, Jitao Chen, Rifang Gu, Min Tan, Ming Tang, Felicity Han, Xuqiang Nie

{"title":"Novel copper-ion coordinated andrographolide-loaded hydrogel activates Rac1/JNK1 axis for enhancing diabetic wound healing.","authors":"Penghui Ye, Yuhe Dai, Qianbo Zhang, Junqi Yang, Lele Liu, Xiuying Guo, Huan Zhu, Jitao Chen, Rifang Gu, Min Tan, Ming Tang, Felicity Han, Xuqiang Nie","doi":"10.1038/s41536-026-00457-y","DOIUrl":"10.1038/s41536-026-00457-y","url":null,"abstract":"Chronic diabetic wounds represent a major clinical challenge, compounded by persistent inflammation, microbial invasion, and deficient angiogenesis. To address these intertwined pathophysiological features, we developed a copper-ion coordinated andrographolide-loaded hydrogel (ASFH), significantly enhancing andrographolide solubility and promoting wound healing dynamics. In vitro assessments demonstrated superior antimicrobial activity, optimal mechanical strength, self-healing ability, and cytocompatibility. In diabetic mice, ASFH notably accelerated wound closure, stimulated collagen maturation and re-epithelialization, dynamically shifted macrophages toward an anti-inflammatory phenotype, and markedly enhanced angiogenesis. Mechanistic studies integrating network pharmacology, molecular docking, dynamics simulations, and SPR validation pinpointed the Rac1/JNK1/Jun/Fos signaling cascade as a primary mediator of these regenerative effects. This work presents ASFH as a translationally relevant dressing system, simultaneously addressing critical limitations in diabetic wound management through targeted molecular therapeutic intervention.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":" ","pages":""},"PeriodicalIF":7.0,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12987937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipose-derived dual cell therapy enhances arteriogenesis and limb preservation through vascular integration in critical limb ischemia. 脂肪源性双细胞治疗通过血管整合在严重肢体缺血中增强动脉生成和肢体保存。

IF 7 1区医学

npj Regenerative Medicine Pub Date : 2026-01-27 DOI: 10.1038/s41536-026-00458-x

Do Young Kim, Dae Yeon Hwang, Gabee Park, Yeon Ju Song, Jiwon Kang, Youngsook Son, Sung Vin Yim, Hyun Sook Hong

{"title":"Adipose-derived dual cell therapy enhances arteriogenesis and limb preservation through vascular integration in critical limb ischemia.","authors":"Do Young Kim, Dae Yeon Hwang, Gabee Park, Yeon Ju Song, Jiwon Kang, Youngsook Son, Sung Vin Yim, Hyun Sook Hong","doi":"10.1038/s41536-026-00458-x","DOIUrl":"10.1038/s41536-026-00458-x","url":null,"abstract":"Peripheral artery disease (PAD) causes progressive arterial narrowing in the lower limbs and can advance to critical limb ischemia (CLI). Limited revascularization options highlight the need for safer, more effective therapies. Vascular multipotent stem cells (VMSCs) and adipose-derived stem cells (ADSCs) were isolated from adipose tissue, characterized phenotypically, and tested for angiogenic activity in vitro. Their therapeutic efficacy was then examined in a murine critical limb ischemia model through intramuscular transplantation, assessing limb preservation, neovascularization, and cell integration. VMSCs shared mesenchymal stem cell-like features with ADSCs and exhibited robust proliferative capacity, enabling rapid expansion to clinically relevant numbers. VMSCs also demonstrated endothelial-like properties, including CD31, VE-cadherin, and CD141 expression, and formed capillary-like structures in vitro. In contrast, ADSCs displayed perivascular characteristics with α-SMA and Transgelin expression. Co-culture of VMSCs and ADSCs promoted the development of mature tubular networks in vitro. Combined cell transplantation markedly decreased limb loss and promoted both angiogenesis and arteriogenesis in ischemic tissue, with transplanted cells partially integrating into the host vasculature to form hybrid vascular structures. VMSCs and ADSCs show complementary regenerative functions, sustained engraftment, and support for large-vessel formation, underscoring their potential for stem cell-based vascular therapies.","PeriodicalId":54236,"journal":{"name":"npj Regenerative Medicine","volume":" ","pages":""},"PeriodicalIF":7.0,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12948985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146068593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0