Journal of Genetic Engineering and Biotechnology最新文献

{"title":"Correlation and regression analysis of KRT35 and TCHHL1 functional genes for cashmere fineness in Liaoning cashmere goats","authors":"Weihang Hong ,&nbsp;Hua Ma ,&nbsp;Lingjun Nie ,&nbsp;Shuaitong Li ,&nbsp;Lingchao Kong ,&nbsp;Ran Duan ,&nbsp;Qingyu Yuan ,&nbsp;Qiying Zhan ,&nbsp;Jinghan Wang ,&nbsp;Yuyan Cong ,&nbsp;Zeying Wang","doi":"10.1016/j.jgeb.2024.100434","DOIUrl":"10.1016/j.jgeb.2024.100434","url":null,"abstract":"<div><div>Liaoning cashmere goat (LCG) is the world’s highest cashmere producing white cashmere goat. It has the characteristics of long cashmere fiber, high net cashmere rate, moderate cashmere fineness, white cashmere, strong size, strong adaptability, stable genetic performance, and good effect in improving middle and low production cashmere goat. It is known as “National treasure of China”. With LCG as the paternal parent, five new local breeds have been cultivated, which has made outstanding contributions to the improvement and breeding of Chinese cashmere goat breeds. LCG cashmere has moderate fineness (the average fineness of cashmere of LCG population is about 16 µm).However, as a slightly coarse textile raw material, we hope to identify the key genes regulating cashmere fineness through PCR-seq and MLR, in order to reduce cashmere fineness.We collected and extracted DNA from the blood of Liaoning cashmere goats, designed primers, PCR amplification, and Statistical analysis. It was found that the the AA genotype of the G3667A locus of the <em>KRT35</em> gene, CT genotype of the T615C locus of the <em>TCHHL1</em> gene in bucks and the CC genotype of does, as well as CT genotype of the T615C locus of the <em>TCHHL1</em> gene in bucks and the CC genotype of does are dominant genotypes in cashmere fineness. The dominant haplotype combination with multiple factors and effects of cashmere fineness has been determined to be CTGG in bucks and TTGG in does. There was a significant linear regression relationship between the fineness of cashmere in LCG and the cashmere rate and cashmere quantity. There is a significant linear regression relationship between the fineness of LCG and the cashmere rate and cashmere quantity. CF = 0.001SQ-0.71CY + 20.784 (R² = 0.818) in buck and CF = 0.001SQ-0.767CY + 22.009 (R² = 0.863) in doe. Conclusion: The AA genotype of <em>KRT35</em> gene, CT genotype of <em>TCHHL1</em> gene in bucks and CC genotype of does can be used as molecular markers to assist in the selection of cashmere fineness.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"22 4","pages":"Article 100434"},"PeriodicalIF":3.5,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142592931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping proteomic response to salinity stress tolerance in oil crops: Towrads enhanced plant resilience","authors":"Sarah Alrajeh ,&nbsp;Muhammad Naveed Khan ,&nbsp;Aidhya Irhash Putra ,&nbsp;Dhafar N. Al-ugaili ,&nbsp;Khalid H. Alobaidi ,&nbsp;Othman Al Dossary ,&nbsp;Jameel R. Al-Obaidi ,&nbsp;Azi Azeyanty Jamaludin ,&nbsp;Mohammed Yahya Allawi ,&nbsp;Bilal Salim Al-Taie ,&nbsp;Norafizah Abdul Rahman ,&nbsp;Norasfaliza Rahmad","doi":"10.1016/j.jgeb.2024.100432","DOIUrl":"10.1016/j.jgeb.2024.100432","url":null,"abstract":"<div><div>Exposure to saline environments significantly hampers the growth and productivity of oil crops, harmfully affecting their nutritional quality and suitability for biofuel production. This presents a critical challenge, as understanding salt tolerance mechanisms in crops is key to improving their performance in coastal and high-salinity regions. Our content might be read more properly: This review assembles current knowledge on protein-level changes related to salinity resistance in oil crops. From an extensive analysis of proteomic research, featured here are key genes and cellular pathways which react to salt stress. The literature evinces that cutting-edge proteomic approaches − such as 2D-DIGE, IF-MS/MS, and iTRAQ − have been required to reveal protein expression patterns in oil crops under salt conditions. These studies consistently uncover dramatic shifts in protein abundance associated with important physiological activities including antioxidant defence, stress-related signalling pathways, ion homeostasis, and osmotic regulation. Notably, proteins like ion channels (SOS1, NHX), osmolytes (proline, glycine betaine), antioxidant enzymes (SOD, CAT), and stress-related proteins (HSPs, LEA) play central roles in maintaining cellular balance and reducing oxidative stress. These findings underline the complex regulatory networks that govern oil crop salt tolerance. The application of this proteomic information can inform breeding and genetic engineering strategies to enhance salt resistance. Future research should aim to integrate multiple omics data to gain a comprehensive view of salinity responses and identify potential markers for crop improvement.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"22 4","pages":"Article 100432"},"PeriodicalIF":3.5,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of key signaling pathways and novel computational drug target for oral cancer, metabolic disorders and periodontal disease","authors":"Mohammad Khursheed Alam ,&nbsp;Md. Faruk Hosen ,&nbsp;Kiran Kumar Ganji ,&nbsp;Kawsar Ahmed ,&nbsp;Francis M. Bui","doi":"10.1016/j.jgeb.2024.100431","DOIUrl":"10.1016/j.jgeb.2024.100431","url":null,"abstract":"<div><h3>Aim</h3><div>Due to conventional endocrinological methods, there is presently no shared work available, and no therapeutic options have been demonstrated in oral cancer (OC) and periodontal disease (PD), type 2 diabetes (T2D), and obese patients. The aim of this study is to determine the similar molecular pathways and potential therapeutic targets in PD, OC, T2D, and obesity that may be used to anticipate the progression of the disease.</div></div><div><h3>Methods</h3><div>Four Gene Expression Omnibus (GEO) microarray datasets (GSE29221, GSE15773, GSE16134, and GSE13601) are used for finding differentially expressed genes (DEGs) for T2D, obese, and PD patients with OC in order to explore comparable pathways and therapeutic medications. Gene ontology (GO) and pathway analysis were used to investigate the functional annotations of the genes. The hub genes were then identified using protein-protein interaction (PPI) networks, and the most significant PPI components were evaluated using a clustering approach.</div></div><div><h3>Results</h3><div>These three gene expression-based datasets yielded a total of seven common DEGs. According to the GO annotation, the majority of the DEGs were connected with the microtubule cytoskeleton structure involved in mitosis. The KEGG pathways revealed that the concordant DEGs are connected to the cell cycle and progesterone-mediated oocyte maturation. Based on topological analysis of the PPI network, major hub genes (CCNB1, BUB1, TTK, PLAT, and AHNAK) and notable modules were revealed. This work additionally identified the connection of TF genes and miRNAs with common DEGs, as well as TF activity.</div></div><div><h3>Conclusion</h3><div>Predictive drug analysis yielded concordant drug compounds involved with T2D, OC, PD, and obesity disorder, which might be beneficial for examining the diagnosis, treatment, and prognosis of metabolic disorders and Oral cancer.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"22 4","pages":"Article 100431"},"PeriodicalIF":3.5,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic silver nanoparticles synthesized using bracken fern inhibits cell proliferation in HCT-15 cells through induction of apoptosis pathway and overexpression of heat shock proteins","authors":"Kailas D. Datkhile ,&nbsp;Pratik P. Durgawale ,&nbsp;Nilam J. Jagdale ,&nbsp;Ashwini L. More ,&nbsp;Satish R. Patil","doi":"10.1016/j.jgeb.2024.100428","DOIUrl":"10.1016/j.jgeb.2024.100428","url":null,"abstract":"<div><h3>Background</h3><div>In recent years, biosynthesized nanoparticles has shown a promise as alternative avenue for improving the effectiveness of conventional chemotherapy. Despite, there is a significant gap in existing literature concerning the comprehensive study of biogenic silver nanoparticles derived from terrestrial fern species and their potential effects on cancer cells. This study is aiming to investigate effects of biogenic silver nanoparticles synthesized using aqueous extract of bracken fern <em>Pteridium revolutum</em> on inhibiting cell proliferation and inducing apoptosis in HCT-15 cells.</div></div><div><h3>Methods</h3><div>Biogenic silver nanoparticles synthesized using aqueous extract of <em>Pteridum revolutum</em> followed by their characterization (UV–Visible spectroscopy, TEM, XRD and FTIR). The impact on cell proliferation of HCT-15 cells was assessed by MTT assay while induction of apoptosis was demonstrated via DNA fragmentation, caspase-3 assay, cell cycle arrest, FITC V- Annexin assay and evaluation of expression of apoptotic genes using real time PCR and western blotting techniques.</div></div><div><h3>Results</h3><div>Results of UV–Vis spectrum of colloidal solution of CW-AgNPs showed surface plasmon resonance peak at 430 nm. TEM and XRD results confirmed synthesis of spherical shaped, 20–40 nm sized nanoparticles. The results elucidate cytotoxic effect of PR-AgNPs against HCT-15 cells in time and dose dependent manner with IC50 observed at 5.79 ± 0.58 µg /mL after 24 h of exposure. Furthermore, PR-AgNPs induce significant alterations in cellular morphology, elevate DNA DNA fragmentation and enhance expression of p53 and caspase-3 in HCT10 cells.</div></div><div><h3>Conclusion</h3><div>The findings from this study address the noteworthy antiproliferative effects of PR-AgNPs in cancer cells primarily mediated through activation of intrinsic apoptosis pathway by inducing p53 and caspase-3 genes.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"22 4","pages":"Article 100428"},"PeriodicalIF":3.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142527094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation and regression analysis of FA2H and ELOVL3 functional genes for cashmere fineness with production performance in Liaoning cashmere goat","authors":"Shuaitong Li ,&nbsp;Lingchao Kong ,&nbsp;Siyi Li,&nbsp;Yining Liu,&nbsp;Yuan Pan,&nbsp;Qingkun Liu,&nbsp;Weihang Hong,&nbsp;Hua Ma,&nbsp;Qingyu Yuan,&nbsp;Ran Duan,&nbsp;Qiying Zhan,&nbsp;Zeying Wang","doi":"10.1016/j.jgeb.2024.100430","DOIUrl":"10.1016/j.jgeb.2024.100430","url":null,"abstract":"<div><div>Liaoning cashmere goat (LCG) is characterized by the highest individual cashmere yield, but its cashmere fineness tends to be coarse. Therefore, our research primarily focuses on reducing cashmere fineness. Through lipidomics screening and identification, we identified the crucial functional genes <em>FA2H</em> and <em>ELOVL3</em> associated with cashmere fineness. Subsequently, using PCR-seq, we conducted gene typing and SNP analysis on the experimental population DNA, In the <em>FA2H</em> gene, a SNP locus T42443G was detected in LCG buck, with the TT genotype showing advantageous traits in cashmere fineness, meat quality, and body size, while the TG genotype demonstrated advantages in slaughter performance,In LCG doe, the TG genotype shows advantageous traits in cashmere fineness, milk production, and meat quality, while the TT genotype exhibits advantages in slaughter performance, lambing, and body size. In the <em>ELOVL3</em> gene, a SNP locus C2133A was identified in LCG buck, where the CC genotype was advantageous for cashmere fineness, Only CA genotype was found in slaughter and meat quality. Additionally, and the CA genotype showed superiority in body size. On LCG doe, The CC genotype was the advantageous genotype in terms of cashmere fineness, milk production, slaughter performance, and meat quality. The CA genotype was the advantageous genotype in terms of lambing and body size. The dominant genotypes identified to influence both doe cashmere fineness and slaughter performance were TT and CC. The identified dominant haplotype combination for cashmere production performance in LCG was CCTG. The dominant haplotype combination for doe slaughter performance was the CCTT haplotype combination. The dominant haplotype combination for buck slaughter performance was the CATG haplotype combination. Therefore, the TT genotype of the <em>FA2H</em> gene and the CC genotype of the <em>ELOVL3</em> gene in LCG buck, and the TG genotype of the <em>FA2H</em> gene and the CC genotype of the <em>ELOVL3</em> gene in doe can be used as molecular markers for assisted selection of cashmere fineness. CCTG haplotype combination was the superior haplotype combinations for cashmere production performance. To provide a theoretical basis for the breeding and expansion of fine-fiber type new strains of LCG.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"22 4","pages":"Article 100430"},"PeriodicalIF":3.5,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142527044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the human cDNA: A descriptive study using library screening in yeast","authors":"Zina Alaswad ,&nbsp;Nayera E. Attallah ,&nbsp;Basma Aboalazm ,&nbsp;Eman S. Elmeslhy ,&nbsp;Asmaa S. Mekawy ,&nbsp;Fatma A. Afify ,&nbsp;Hesham K. Mahrous ,&nbsp;Ashrakat Abdalla ,&nbsp;Mai A. Rahmoon ,&nbsp;Ahmed A. Mohamed ,&nbsp;Ahmed H. Shata ,&nbsp;Rana H. Mansour ,&nbsp;Fareed Aboul-ela ,&nbsp;Mohamed Elhadidy ,&nbsp;Biola M. Javierre ,&nbsp;Sherif F. El-Khamisy ,&nbsp;Menattallah Elserafy","doi":"10.1016/j.jgeb.2024.100427","DOIUrl":"10.1016/j.jgeb.2024.100427","url":null,"abstract":"<div><div>The utilization of human cDNA libraries in yeast genetic screens is an approach that has been used to identify novel gene functions and/or genetic and physical interaction partners through forward genetics using yeast two-hybrid (Y2H) and classical cDNA library screens. Here, we summarize several challenges that have been observed during the implementation of human cDNA library screens in <em>Saccharomyces cerevisiae</em> (budding yeast). Upon the utilization of DNA repair deficient-yeast strains to identify novel genes that rescue the toxic effect of DNA-damage inducing drugs, we have observed a wide range of transcripts that could rescue the strains. However, after several rounds of screening, most of these hits turned out to be false positives, most likely due to spontaneous mutations in the yeast strains that arise as a rescue mechanism due to exposure to toxic DNA damage inducing-drugs.</div><div>The observed transcripts included mitochondrial hits, non-coding RNAs, truncated cDNAs, and transcription products that resulted from the internal priming of genomic regions. We have also noticed that most cDNA transcripts are not fused with the GAL4 activation domain (GAL4AD), rendering them unsuitable for Y2H screening. Consequently, we utilized Sanger sequencing to screen 282 transcripts obtained from either four different yeast screens or through direct fishing from a human kidney cDNA library. The aim was to gain insights into the different transcription products and to highlight the challenges of cDNA screening approaches in the presence of a significant number of undesired transcription products. In summary, this study describes the challenges encountering human cDNA library screening in yeast as a valuable technique that led to the identification of important molecular mechanisms. The results open research venues to further optimize the process and increase its efficiency.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"22 4","pages":"Article 100427"},"PeriodicalIF":3.5,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatics analysis identifies WNK1 gene as a potential biomarker for cholangiocarcinoma diagnosis and immune infiltration","authors":"Qi Sun ,&nbsp;Xianli Lei ,&nbsp;Xiangrong Meng ,&nbsp;Caijun Zha ,&nbsp;Lei Yan ,&nbsp;Wenjing Zhang","doi":"10.1016/j.jgeb.2024.100426","DOIUrl":"10.1016/j.jgeb.2024.100426","url":null,"abstract":"<div><h3>Background</h3><div>Cholangiocarcinoma (CHOL) is a malignant epithelial carcinoma of the digestive system with poor prognosis and high mortality. WNK lysine deficient protein kinase 1 (WNK1) is known to be associated with tumorigenesis in various cancers. However, the relationship between WNK1 and CHOL development, as well as the potential mechanisms involved, remains poorly understood.</div></div><div><h3>Methods</h3><div>Microarray datasets of CHOL (GSE22633 and GSE32879) were retrieved from the Gene Expression Omnibus (GEO) database. Functional enrichment and immunoinfiltration analyses were performed for genes co-expressed with WNK1. GraphPad Prism 9 was utilized for statistical data analysis and the construction of receiver operating characteristic (ROC) curves. The impact of WNK1 on the CHOL tumor microenvironment was analyzed using Tumor Immune Estimation Resource (TIMER), Venn diagrams, STRING, and TISIDB database for information on WNK1-related chemokines and chemokine receptors. Protein-protein interaction (PPI) networks were used to predict transcription factors and microRNAs interacting with WNK1 and the associated hub genes.</div></div><div><h3>Results</h3><div>Differential expression of WNK1 was observed between CHOL and normal samples, suggesting its diagnostic value. Functional analysis showed that WNK1 and its associated genes were primarily enriched in pathways such as leukocyte transendothelial migration and chemokine signaling. Neutrophils were the only type of infiltrating immune cells associated with WNK1 in the CHOL tumor microenvironment (TME). <em>VEGFA</em> and <em>ALB</em> were identified as hub genes, and X-C motif chemokine receptor 1 (XCR1) and C-X-C motif chemokine ligand 5 (CXCL5) were identified as core chemokines and chemokine receptors related to WNK1 and neutrophil infiltration in CHOL.</div></div><div><h3>Conclusions</h3><div>Based on network analysis and the summary of previous studies, it was proposed that CHOL tumor cells secrete CXCL5, leading to neutrophil recruitment to the tumor microenvironment. Vascular endothelial growth factor A (VEGFA) released by the infiltrating neutrophils is suggested to promote overexpression of WNK1 by tumor cells, activating the VEGFA downstream pathway to promote angiogenesis and tumor progression.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"22 4","pages":"Article 100426"},"PeriodicalIF":3.5,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological characterization and genetic diversity of mini core collection of Rosa damascena from Morocco","authors":"Nezha Lebkiri ,&nbsp;Younes Abbas ,&nbsp;Driss Iraqi ,&nbsp;Fatima Gaboun ,&nbsp;Karim Saghir ,&nbsp;Mohamed Fokar ,&nbsp;Ismail El hamdi ,&nbsp;Khadija Bakhy ,&nbsp;Rabha Abdelwahd ,&nbsp;Ghizlane Diria","doi":"10.1016/j.jgeb.2024.100423","DOIUrl":"10.1016/j.jgeb.2024.100423","url":null,"abstract":"<div><div>Damask rose (<em>Rosa damascena</em> Mill.) is a popular Rosa species cultivated for the industrial production of rose oil worldwide. In the current study, the morphological characteristics and genetic diversity among 12 populations of these species collected from Kelaat M’gouna in Morocco were examined to identify more variable traits and compare their genetic structure. Observations were recorded for a total of 24 morphological traits. The phenotypic variation coefficient (CV) of the studied traits varied from 4.79 % to 42.52 %, confirming the high phenotypic variation between accessions. Cluster analysis grouped accessions into two major clusters based on their morphological resemblance. For molecular investigations, nuclear DNA was amplified using 13 ISSR markers. Analysis of molecular variance (AMOVA) indicated that the highest proportion was within populations (87 %) rather than between them (13 %). Boutaghrar region recorded high values of genetic diversity (He = 0.237), percentage polymorphic loci (PPL = 67 %) and Shannon information index (I = 0.358) Clustering based on Jaccard similarity divided studied the accessions into three distinct clusters. STRUCTURE analysis and principal coordinate analysis (PCoA) were consistent with the genetic relationships derived from cluster analysis. Our study suggests that the wide genetic variation and haplotype observed in Moroccan Damascene roses are valuable for future improvement and conservation of rose programs, particularly for enhancing commercial traits, flower yield, and breeding stability.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"22 4","pages":"Article 100423"},"PeriodicalIF":3.5,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1687157X24001264/pdfft?md5=ad56b0a7150b88b03593fc0686de25ac&pid=1-s2.0-S1687157X24001264-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The positive implication of natural antioxidants on oxidative stress-mediated diabetes mellitus complications","authors":"Shouvik Mallik ,&nbsp;Bijoy Paria ,&nbsp;Sayed Mohammad Firdous ,&nbsp;Hesham S. Ghazzawy ,&nbsp;Nashi K. Alqahtani ,&nbsp;Yong He ,&nbsp;Xiaoli Li ,&nbsp;Mostafa M. Gouda","doi":"10.1016/j.jgeb.2024.100424","DOIUrl":"10.1016/j.jgeb.2024.100424","url":null,"abstract":"<div><p>The complementary intervention to modulate diabetes mellitus (DM) metabolism has recently brought the global attention, since DM has become among the global burden diseases. Where, several related pathways elevate the production of superoxide in consequences. For example, the flux of glycation-derived end products (AGEs) could lead to the deactivation of insulin signaling pathways. In that context, many vitamins and phytochemicals in natural sources have high antioxidant impacts that reduce oxidative stress and cell damages. These chemicals could be applied as bioactive antidiabetic agents. Their mode of actions could be from regulating the intracellular reactive oxygen species (ROS) which cause several pro-inflammatory pathways related to the oxidative stress (OS) and DM. Besides, they have a great potential to control the epigenetic mutations and hyperglycemia and help in back the blood glucose to the normal level. Therefore, the current review addresses the important role of natural functional antioxidants in DM management and its association with its OS complications.</p></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"22 4","pages":"Article 100424"},"PeriodicalIF":3.5,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1687157X24001276/pdfft?md5=003949e7bb5c750d4d6a74f61c9b5f9f&pid=1-s2.0-S1687157X24001276-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142162427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opuntia ficus indica cladode extract inhibit DNA double-strand breaks and locally multiply damaged sites induced by gamma radiation","authors":"Saloua Kouass Sahbani","doi":"10.1016/j.jgeb.2024.100425","DOIUrl":"10.1016/j.jgeb.2024.100425","url":null,"abstract":"<div><p>It is beyond doubt that radiotherapy is extremely effective in treating a wide variety of cancers. The sensitivity of the surrounding normal tissues limits the amount of radiation administered to the tumor. There is an urgent need to develop a treatment that combines pharmacological treatment with ionizing radiation (IR) specifically designed to specifically target cancer cells while protecting the surrounding normal tissue, resulting in an increase in the efficacy of the cancer treatment. IR could cause many types of DNA lesions. Double-strand breaks (DSBs) and<!--> <!-->locally multiple damaged sites (LMDS)<!--> <!-->are<!--> <!-->the main radiotoxic damages.<!--> <!-->Recently, the identification of new antioxidants from natural sources has attracted the attention of scientists. In this context, the present study aims to determine if the <em>Opuntia ficus indica</em> cladode extract (CE) can be used as a radioprotector.</p></div><div><h3>Materials and methods</h3><p>The DNA treated by ¹³⁷Cs γ-radiation (25–700 Gy) in the absence or presence of cactus cladode extract (CCE) was added to the<!--> <em>E. coli</em> <!-->base excision repair. The amounts of both DNA damages were calculated using the electrophoretic method.</p></div><div><h3>Results</h3><p>The irradiation of DNA in the presence of CCE induced a dramatic decrease of the yields of purine and pyrimidine-DSB. A decrease of<!--> <!-->65 % and 84 % of the purine and pyrimidine-DSB sensitive sites have been calculated, respectively, when the sample added CCE3 during the radiotreatment. Moreover, a reduction of 80 % in the amount of Nth + Fpg-DSB SSs (non-DSB cluster damage) after γ-irradiation in the presence of CCE3 was observed.</p></div><div><h3>Conclusion</h3><p>Through the present it was found that the CCE can play an important role as a radio protector, maybe by scavenging the ROS formed during radio treatment or by other unknown pathways. The most toxic DNA lesions (DSBs, and LMDS) decreased dramatically. Studies aimed at obtaining more documentation about CCE components with potential radio-preventive activity are desirable because of their protective properties.</p></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"22 4","pages":"Article 100425"},"PeriodicalIF":3.5,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1687157X24001288/pdfft?md5=91cc4f47f63cfed3027ae673f3c0b47f&pid=1-s2.0-S1687157X24001288-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142150173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}