Journal of Genetic Engineering and Biotechnology最新文献

{"title":"Gamma-Aminobutyric acid profiling (GABA) and standardization of in-vitro regeneration protocol in tomato (Solanum lycopersicum L.) genotypes","authors":"D. Ram Babu ,&nbsp;Zakir Hussain ,&nbsp;Praveen Kumar Singh ,&nbsp;Suman Lata ,&nbsp;Alka Joshi ,&nbsp;Navinder Saini ,&nbsp;T.S. Chaithra","doi":"10.1016/j.jgeb.2025.100624","DOIUrl":"10.1016/j.jgeb.2025.100624","url":null,"abstract":"<div><div>This present study focused on assessing Gamma-aminobutyric acid (GABA) levels in seventy <em>kharif</em> tomato genotypes to highlight its importance in neurotransmission. The experiment was performed at ICAR- Indian Agricultural Research Institute, New Delhi, during 2021–2022. Analysis revealed that most genotypes exhibited a decline in GABA content from the green to turning stages. However, the yellow and orange fruited genotypes K-69 and K-70 consistently maintained stable GABA levels throughout ripening, underscoring their potential for biofortification. In this experiment, efforts were also made to use <em>in-vitro</em> regeneration methods, emphasising proper sterilization technique. Among the different sterilization techniques, NaOCl 2 % for 10 min showed less contamination. Treatment T₇ (BAP 2 mg/l, NAA 1.5 mg/l, IBA 0.5 mg/l) was most effective for callus induction, while T₅ (BAP 1 mg/l, NAA 0.5 mg/l, IBA 2 mg/l) showed maximum shoot multiplication rate and average number of roots per shoot. Explant length and precise growth regulator combinations were found to be critical factors influencing successful regeneration outcomes. The study encountered challenges such as genotype-dependent variability in tissue culture response and contamination risks, which limited reproducibility across genotypes. Nevertheless, the identification of GABA-stable tomato genotypes and optimized regeneration protocols provides important insights into the relationship between fruit ripening and GABA metabolism. These findings not only advance tissue culture applications for trait improvement and cultivar development but also highlight the nutritional and health benefits of enhancing GABA content in tomato, thereby linking agricultural biotechnology with human health promotion.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 1","pages":"Article 100624"},"PeriodicalIF":2.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145665530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI based multiomics integration for cancer diagnosis and prognosis","authors":"Moshira Ghaleb,&nbsp;Maryam Al-Berry,&nbsp;Hala Ebied,&nbsp;Mohamed Tolba","doi":"10.1016/j.jgeb.2025.100639","DOIUrl":"10.1016/j.jgeb.2025.100639","url":null,"abstract":"<div><div>Cancer remains a critical global health challenge, driving the need for innovative approaches in diagnosis and treatment. This research introduces OmicsFusionNet, an AI-powered hybrid model integrating machine learning and deep learning to revolutionize cancer care. The tool incorporates up to six multiomics datasets—genomics, transcriptomics, and epigenomics—achieving 80.2% accuracy across 23 cancer types. Notably, RNAseq and methylation integration reached 99.8% accuracy, highlighting XGBoost’s feature selection and deep learning’s classification strength.</div><div>For ovarian cancer stage detection, OmicsFusionNet optimized analysis using CPTAC-OV and TCGA-OV datasets, achieving accuracies between 83% and 91% by combining ElasticNet and XGBoost with deep learning. Additionally, KEGG pathway enrichment of multiomics biomarkers identified key cancer-related pathways, advancing early detection, biomarker discovery, and personalized treatments.</div><div>This study underscores the transformative potential of AI and multiomics integration in cancer research, enabling precise interventions and uncovering novel mechanisms that enhance patient outcomes.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 1","pages":"Article 100639"},"PeriodicalIF":2.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Broccoli-mediated gold nanoparticles: Eco-friendly synthesis and nano-bio interactions promoting wound healing and targeted cytotoxicity","authors":"Yasser M. Taay ,&nbsp;Mustafa Taha Mohammed ,&nbsp;Ali Hussain Alwan ,&nbsp;Ahmad Hussein Ismail","doi":"10.1016/j.jgeb.2025.100635","DOIUrl":"10.1016/j.jgeb.2025.100635","url":null,"abstract":"<div><div>Biogenic gold nanoparticles (AuNPs) were synthesized using broccoli extract to assess their antioxidant activity, wound-healing potential, and selective anticancer effects. Green synthesis with broccoli offers an environmentally friendly way to produce stable and biocompatible nanomaterials. In this study, <em>Brassica oleracea</em> aqueous extract served as both the reducing and capping agent, producing AuNPs with a characteristic surface plasmon resonance peak at 560 nm and a well-defined cubic crystalline structure confirmed by XRD. TEM analysis showed uniformly dispersed, semi-spherical nanoparticles with an average size of 7.5 ± 3.6 nm. The biosynthesized AuNPs exhibited potent antioxidant activity, achieving 91.2 % DPPH scavenging at 100 µg/mL. <em>In vivo</em> tests demonstrated significantly faster wound healing, with approximately 90 % contraction by day 7 compared to 60 % in the control group, supported by histological evidence of increased collagen deposition and complete epithelialization. Moreover, AuNPs showed selective cytotoxicity towards HepG2 cancer cells (IC₅₀ = 53.45 µg/mL) while maintaining over 70 % viability in normal HDF cells. This selectivity likely stems from differences in redox balance and mitochondrial vulnerability between cancer and healthy cells. Overall, the study presents broccoli-mediated AuNPs as multifunctional nanomaterials with potent antioxidant, regenerative, and targeted anticancer properties, highlighting their promising potential in biomedical applications.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 1","pages":"Article 100635"},"PeriodicalIF":2.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct organogenesis and genetic fidelity of papaya ‘Red Lady’ with improved acclimatization using foliar silicon and salicylic acid","authors":"Manal E. Ahmed ,&nbsp;Walaa M. Ibrahim ,&nbsp;Reda E.E. Abo El-Fadl ,&nbsp;Heba A.M. Abdalla ,&nbsp;Yara S Abuhashem ,&nbsp;Tamer M. Abd Elaziem","doi":"10.1016/j.jgeb.2025.100632","DOIUrl":"10.1016/j.jgeb.2025.100632","url":null,"abstract":"<div><h3>Background</h3><div>Micropropagation of Papaya (<em>Carica papaya</em> L.) cultivar ’Red Lady’, a commercially the important tropical fruit crop, is a highly efficient biotechnological tool for the mass production of disease-free, genetically uniform, and high-quality planting material compared to conventional propagation methods that cause genetic segregation and disease susceptibility. SCoT is a vital molecular marker for detect genetic stability in plant micropropagation protocols. Accordingly, using SCoT markers to assess genetic stability among <em>carica papaya</em> L. cultivar ‘Red Lady’ plantlets.</div></div><div><h3>Results</h3><div>The present study aimed to develop an efficient <em>in vitro</em> propagation protocol for papaya using leaves. The highest survival percentage, mean number and length of adventitious buds of papaya leaf segments (97.00 ± 2 %, 119.00 ± 1.53 and 4.8 ± 0.4 cm, respectively) were achieved by using 1.0 mg/l of <em>meta</em>-topoline (<em>m</em>T) after 12 weeks of culture. On the other hand, using 0.5 g/l <em>m</em>T without activated charcoal (AC) showed to give the highest shoot numbers/ explant (73.50 ± 1.0). During rooting stage, using 0.50 mg/l of IBA in combination with NAA at 0.25 mg/l is the most promising treatment that gave the highest significant percentage of rooting (74 ± 2.64 %). In acclimatization stage, 200 ppm silicon (Si) showed to give the most promising results for survival percentage (85 %) that were foliar sprayed and watered acclimatized plantlets with it.</div><div>In current study, plantlets obtained by direct organogenesis, eight SCoT markers generated a total of 704 amplicons. Plantlets showed 0.90 genetic uniformity from mother plants during using 8 SCoT markers. These findings strongly support our micropropagation techniques is an vital methodology to generation <em>carica papaya</em> L. cultivar ‘Red Lady’.</div></div><div><h3>Conclusion</h3><div>Compared with other <em>in vitro</em> propagation methods, direct organogenesis offers a faster and more reliable approach for producing true-to-type papaya plants. This regeneration pathway proved to be efficient, reproducible, and genetically stable, as no somaclonal variation was observed among the regenerated plantlets. Our protocol is controlled and reliable, as adventitious shoots transplanted more than 8 times are consistent with the genetic stability of first-generation donor material. Therefore, Si application can be considered an effective and co-friendly strategy to improve the success rate of acclimatization in tissue cultured plants. The findings of the study proved that there is a significant correlation between physiological parameters (CAT, MDA, stomatal density) and survival percentage.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 1","pages":"Article 100632"},"PeriodicalIF":2.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145694616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatics-Guided structural characterization and immunogenicity assessment of multi-epitope vaccine candidates against Zika virus","authors":"Sirajul Islam,&nbsp;Md. Akramul Haque,&nbsp;Md. Sakhawat Hossain,&nbsp;Md. Al Amin,&nbsp;Shahin Mahmud","doi":"10.1016/j.jgeb.2025.100641","DOIUrl":"10.1016/j.jgeb.2025.100641","url":null,"abstract":"<div><div>The Zika virus (ZIKV) causes a significant global health threat, necessitating the development of effective vaccines. In this study, using bioinformatics and software-based vaccinology approaches, firstly we evaluated the antigenicity of all proteins of ZIKV (EU545988.1), identifying Protein Pr, E, and NS1 as the most antigenic. Subsequent investigation into the antigenic profiles of these proteins across the top 10 infectious ZIKV strains unveiled Strain Zika (USA, KU312312), Rio-U1 (Brazil, KX601166), and Zika (Brazil, KU321639) as harboring the highest antigenicity for Protein Pr (0.7194555), E (0.6559635), and NS1 (0.6115945), respectively. Next, T cell and B cell epitopes were predicted for these proteins, and their antigenicity, allergenicity, and toxicity were evaluated. Binding affinity of T cell epitopes with MHC I and II molecules was determined. Population coverage and conservancy analysis were conducted. Three B cell epitopes (ATMSYECPMLDHVQI, TMSGKAISFATTLG, and KDAHAKRQTVYVCKR) and three T cell epitopes (DLGHTCDATMSYECP, ALGGVMIFLSTAVSA, and TSVWLKYHPDSPRRL) from Protein Pr, E, and NS1 were identified as optimal vaccine candidates. Among them, ALGGVMIFLSTAVSA and TSVWLKYHPDSPRRL showed 100% conservancy and 71.88% and 95.24% Population coverage (worldwide) where DLGHTCDATMSYECP showed 95.24% Conservancy and 94.77% Population coverage. Using them, the multi-epitope vaccine (MEV) was constructed with an appropriate adjuvant (<span><span>P29459</span><svg><path></path></svg></span>) and linkers, including a His-tag for ease of isolation during in vivo procedures. Secondary and tertiary structures of the MEV were predicted, and physiochemical properties and subcellular localization were analyzed. Furthermore, allergenicity, toxicity and immune simulation of the MEV were assessed. Molecular docking analysis confirmed binding affinity with human receptor proteins TLR3 (PDB: <span><span>7C76</span><svg><path></path></svg></span>) and stability was evaluated through Molecular Dynamics Simulation analysis. Post-translational modifications analysis was conducted, and in silico cloning in <em>E. coli</em> was performed to assess cloning feasibility of the MEV. This integrative approach provides insights into the development of a potential ZIKV vaccine, laying the groundwork for further wet lab validation and preclinical and clinical investigations.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 1","pages":"Article 100641"},"PeriodicalIF":2.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive gene profiling by Next-Generation sequencing in a cohort of Egyptian pediatric Atypical HUS","authors":"Fatina I. Fadel ,&nbsp;Khalda Amr ,&nbsp;Rasha EssamEldin Galal ,&nbsp;Ghada El-kamah ,&nbsp;Mohamed A Abdel Mawla ,&nbsp;Amr Mohamed Salem ,&nbsp;Mohamed S. Thabet ,&nbsp;Shorouk A.Othman","doi":"10.1016/j.jgeb.2025.100656","DOIUrl":"10.1016/j.jgeb.2025.100656","url":null,"abstract":"<div><h3>Background</h3><div>Atypical hemolytic uremic syndrome (aHUS) is a rare, severe condition in children, resulting from uncontrolled complement activation caused by genetic factors or autoantibodies; it usually has a poor prognosis. Early identification of the underlying genetic variants is crucial for guiding management and predicting outcomes. This study aimed to identify gene mutations in Egyptian children with aHUS at an early stage. This would enable the selection of the most appropriate treatment options and help prevent adverse outcomes for the patients<strong>.</strong></div></div><div><h3>Methods</h3><div>This is an observational cohort study, included 21 children with a clinical diagnosis of aHUS who presented to the Pediatric Nephrology Unit and general wards of Cairo University Children’s Hospital between June 2022 and January 2024 with a follow-up duration of 12 months (median). All patients underwent whole exome sequencing (WES). Clinical data, treatment regimens, and outcomes were recorded and analyzed.</div></div><div><h3>Result</h3><div>Among the 21 patients, around one third had negative WES results while 28.57 % showed CFHR 3/CFHR1 deletion. Most patients progressed to chronic kidney disease (52.4 %), while 28.6 % recovered their kidney functions following plasmapheresis. A significant association was observed between WES category and disease relapse (p = 0.021); patients with CFHR3 deletion, CFHR5 deletion, or MMUT variant developed at least one attack of relapse.</div></div><div><h3>Conclusions</h3><div>Egyptian children with aHUS demonstrate marked genetic heterogeneity. A substantial proportion lacked identifiable pathogenic variants, highlighting the complexity of the disease. CFHR3/CFHR1 deletion was the most frequent finding. Genetic profiling remains crucial for anticipating relapse risk and guiding therapeutic decisions, particularly in resource-limited settings.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 1","pages":"Article 100656"},"PeriodicalIF":2.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145884541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic analysis of bacterial and viral communities of Aedes aegypti and Aedes albopictus","authors":"Shahzadi Asia Nadeem ,&nbsp;Ijaz Ali ,&nbsp;Hazrat Hussain ,&nbsp;Ihsan Ullah ,&nbsp;Wajid Ali ,&nbsp;Khalid J. Alzahrani ,&nbsp;Hamid Ali ,&nbsp;Zarak Imtiaz Khan ,&nbsp;Kasim Sakran Abass ,&nbsp;Rafi ur Rahman","doi":"10.1016/j.jgeb.2025.100643","DOIUrl":"10.1016/j.jgeb.2025.100643","url":null,"abstract":"<div><h3>Background</h3><div>The complicated relationship between the <em>Aedes</em> mosquito microbiome, arbovirus transmission and essential physiological processes, is extremely important. Microbial community plays a vital role in shaping vector biology, impacting critical aspects such as parasite replication within the vector, vector longevity, and ultimately, vector competence. Understanding the composition and function of the <em>Aedes</em> microbiome is therefore crucial for developing novel strategies to control arboviral diseases. Therefore, we aimed to identify prevalent bacterial and viral communities in <em>Aedes</em> mosquitoes from Pakistan.</div></div><div><h3>Methods</h3><div><em>Ae. aegypti</em> and <em>Ae. albopictus</em> were collected and from three different regions of Khyber Pakhtoonkhwa, Punjab and federal capital Islamabad. We isolated DNA and sequenced two pools of each species and conducted metagenomic analysis, identifying a variety of bacteria and viruses.</div></div><div><h3>Results</h3><div>We found diverse bacterial and viral communities in both studied species. In <em>Ae. aegypti</em>, the most abundant bacterial species was <em>Klebsiella pneumoniae</em> followed by <em>Acinetobacter baylyi</em>. <em>Ae. albopictus</em> presented <em>Pseudomonas putida</em> as the most abundant bacterial species followed by <em>Brevundimonas diminuta</em>. Similarly in <em>Ae. aegypti</em>, we found that <em>Escherichia phage</em> HK639 was the most abundant viral species while in <em>Ae. albopictus</em>, it was <em>Lactobacillus phage</em> 2. It is important to mention that the prevalent viruses in both <em>Aedes</em> species belong to the <em>Siphoviridae</em> genus.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 1","pages":"Article 100643"},"PeriodicalIF":2.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic SSR markers elucidate genetic architecture and phenotypic trait associations in Rhododendron Linnaeus","authors":"Jianshuang Shen ,&nbsp;Yuanjun Ye ,&nbsp;Yiwei Zhou ,&nbsp;Xianlin Rong","doi":"10.1016/j.jgeb.2025.100631","DOIUrl":"10.1016/j.jgeb.2025.100631","url":null,"abstract":"<div><div>The genus <em>Rhododendron</em> Linnaeus, comprising over 1,000 species distributed across temperate and alpine regions of Asia, Europe, and North America, holds significant value for its ornamental characteristics, ecological adaptability, and medicinal potential. Despite its importance, the genus presents persistent challenges in taxonomic classification and phylogenetic resolution, compounded by the current insufficiency of genomic SSR markers (g-SSRs) for comprehensive genetic studies. In this study, we conducted the first comprehensive genome-wide identification of SSR markers using whole-genome sequencing data of <em>Rhododendron</em> × <em>pulchrum</em> Sweet. Our analysis identified 324,674 SSR loci across the 509.49 Mb genome. From 190 synthesized primer pairs, 124 (65.3 %) demonstrated successful amplification, with 30 (24.2 %) showing high polymorphism. These polymorphic g-SSR markers generated 597 polymorphic bands, exhibiting polymorphic information content (PIC) values ranging from 0.240 to 0.949 (mean = 0.748), reflecting substantial genetic diversity among the studied accessions. Genetic structure, cluster analysis, and principal coordinates analysis of 96 <em>Rhododendron</em> accessions revealed three distinct clades that correlated with geographic origins, hybrid relationships, and utilization values. Association mapping identified 17 SSR markers significantly linked (<em>P</em> &lt; 0.05) to 10 key morphological traits, explaining 5.68–13.35 % of phenotypic variance. The number of significantly associated markers for each trait was as follows: leaf length (1), remontant (3), corolla lobe shape (2), initial flowering period (3), flower diameter (3), leaf shape (1), petal type (3), petiole length (2), stamen number (4), and flower shape (1). This study provides valuable genomic resources for marker-assisted breeding and establishes a foundation for systematic characterization, conservation, and utilization of <em>Rhododendron</em> genetic resources.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 1","pages":"Article 100631"},"PeriodicalIF":2.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145694617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of potential key genes and molecular mechanisms of oral squamous cell carcinoma based on integrated bioinformatics approach","authors":"Mohammad Khursheed Alam ,&nbsp;Md Rakibul Islam ,&nbsp;Tahsinul Haque ,&nbsp;Kiran Kumar Ganji ,&nbsp;Hamzah Ali Babkair ,&nbsp;Mohammed Enamur Rashid ,&nbsp;Kawsar Ahmed ,&nbsp;Francis M. Bui","doi":"10.1016/j.jgeb.2026.100668","DOIUrl":"10.1016/j.jgeb.2026.100668","url":null,"abstract":"<div><div>Oral Squamous Cell Carcinoma (OSCC) is one of the most occurred cancer types with yearly 377,713 cases and 177,000 deaths. Traditional risk factors of OSCC include smoking, alcohol consumption, excessive sun exposure, family history of cancer, and human papillomavirus (HPV). Last few years, the prevalence of OSCC is growing big in numbers particularly among younger people for their lifestyle. From the Gene Expression Omnibus, 2 gene-expression profiles (GSE23558 and GSE146483) were identified based on some conditions. The GEO2R tool was used to analyze those datasets to extract all the genes. Statistical cut-off criteria were applied to find out DEGs from both datasets, and after that common DEGs were identified by comparing both datasets. Common DEGs were used to perform bioinformatics analysis such as gene ontology and pathway analysis, protein–protein interaction (PPI) network construction, and generating Transcription factor − miRNA network. 265 common DEGs were identified from the datasets including 69 up-regulated and 196 down-regulated DEGs. Using the STRING database and a strong combine score &gt; 0.70, a PPI network is generated including 92 nodes and 226 interactions. Using 3 different hub DEGs seeking algorithm, we identified 9 top hub DEGs. The hub genes are Kinesin Family Member 23 (KIF23), Aurora Kinase A (AURKA), Centromere Protein F (CENPF), Cell Division Cycle 20 (CDC20), Discs Large Associated Protein 5 (DLGAP5), Centrosomal Protein 55 (CEP55), Anillin Actin Binding Protein (ANLN), Non-SMC Condensin I Complex Subunit G (NCAPG), and Kinesin Family Member 14 (KIF14). 3 significant clusters also identified from the PPI network. Previous study shows KIF23 takes part in raising Cell Proliferation in Hepatocellular carcinoma cells and AURKA shows notable overexpression in cancer tissues, which indicates that KIF23 and AURKA showed promising character to become possible biomarkers for OSCC. Further analysis needed to justify the statement.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 1","pages":"Article 100668"},"PeriodicalIF":2.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147395710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of somatic embryogenesis protocol for Hevea brasiliensis clones RRIM 600 and REYAN 88-13","authors":"Florence Dessailly ,&nbsp;Li Zhe ,&nbsp;Florence Martin ,&nbsp;Julie Petit-Briand ,&nbsp;Pascal Montoro ,&nbsp;Julie Leclercq","doi":"10.1016/j.jgeb.2025.100640","DOIUrl":"10.1016/j.jgeb.2025.100640","url":null,"abstract":"<div><div>The challenges for the development of rubber cultivation are so agronomically significant in the face of climate change that it is crucial to introduce biotechnology into the clonal propagation of rubber trees. A protocol was developed on rubber clone PB 260. This protocol was used for two other clones, RRIM 600 and REYAN 88-13, which exhibited highly contrasting response to callus growth and embryogenic capacity. Optimization of somatic embryogenesis for these two clones led us to successful conduct genetic transformation trials. We discussed the <em>in vitro</em> behaviour of the calli considering the origin of the embryogenic callus and the endogenous redox status.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 1","pages":"Article 100640"},"PeriodicalIF":2.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}