Journal of Genetic Engineering and Biotechnology最新文献_第10页

Buffalo species identification and delineation using genetic barcoding markers 利用遗传条形码标记进行水牛物种鉴定和圈定

IF 3.5

Journal of Genetic Engineering and Biotechnology Pub Date : 2018-12-01 DOI: 10.1016/j.jgeb.2018.07.006

Amal Ahmed Mohamed Hassan , Esraa Aly Balabel , Hanaa Abdel Sadek Oraby , Samy Anwar Darwish

{"title":"Buffalo species identification and delineation using genetic barcoding markers","authors":"Amal Ahmed Mohamed Hassan , Esraa Aly Balabel , Hanaa Abdel Sadek Oraby , Samy Anwar Darwish","doi":"10.1016/j.jgeb.2018.07.006","DOIUrl":"10.1016/j.jgeb.2018.07.006","url":null,"abstract":"<div><p>Enrichment of barcode databases with mitochondrial cytochrome <em>c</em> oxidase subunit I (COI) barcode sequences in different animal taxa has become important for identification of animal source in food samples to prevent commercial fraud. In this study, COI barcode sequence in seventy one river buffalo samples were determined, analyzed and deposited in Genbank barcode database and barcode of life database (BOLD) to contribute for construction of public reference library for COI barcode sequence in river buffalo. Moreover COI barcode sequence was used to identify the closely related buffalo groups: river buffalo, swamp buffalo, lowland anoa and African buffalo. Results indicated the success of the COI barcode in the identification of each of the tested groups. Whereas a suggested sequence of other mitochondrial segment representing two successive transfer RNA (tRNA) genes; tRNA-Threonine (MT-TT) and tRNA-Proline (MT-TP) was failed to be used as a barcode marker for differentiation between the tested buffalo groups.</p></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jgeb.2018.07.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36985823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Detection of myostatin gene MSTN in some goat breeds (Capra hircus) 部分山羊品种肌肉生长抑制素基因MSTN的检测

IF 3.5

Journal of Genetic Engineering and Biotechnology Pub Date : 2018-12-01 DOI: 10.1016/j.jgeb.2018.04.002

Y.A. Dowidar , M.A. El-Sayed , Aly M. Elrefy , Hytham E. Shoura

{"title":"Detection of myostatin gene MSTN in some goat breeds (Capra hircus)","authors":"Y.A. Dowidar , M.A. El-Sayed , Aly M. Elrefy , Hytham E. Shoura","doi":"10.1016/j.jgeb.2018.04.002","DOIUrl":"10.1016/j.jgeb.2018.04.002","url":null,"abstract":"<div><p>Till now not information about myostatin MSTN gene in Egyptian goat breeds. Here we show more information about MSTN in some Egyptian goat breeds to enrich the database with new sequences for Egyptian goat breeds. Our conducted study focused on detection and identifying the MSTN gene as a candidate gene of the muscles growth trait in three goat breeds (Zaraibi, Baladi and Damascus). We found the similarity between the registered sequences with the accession numbers KY463684 for Zaraibi and KY463685 for Baladi and Chinese goat breeds of the MSTN gene deposited with international gene banks by up to 99% and some other species including sheep, cows and bull breeds with percentages of 95 to 97% and between 95 to 99%, respectively. There is also a correlation between the sequences of the registered pieces of Baladi with KY463686 and Damascus and Chinese breeds with KY441464 of MSTN deposited with international gene banks by up to 99% and some other species including sheep and bull breeds at a ratio of 99% for two pieces. Results demonstrated the deposited sequences of object are part of intron 1, exon 2 is fully sequenced with Zaraibi and Baladi breeds; the intron 1, exon 1 with Baladi breed; and the intron 2, part of exon 3 with Damascus breed. Therefore, the Egyptian goat breeds consider national wealth can be used to develop breeding and improvement programs which helps in more applicable scopes like biotechnology, genetic engineering and molecular biology with the help of bioinformatics tools.</p></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jgeb.2018.04.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36985824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Transgenic approaches for genetic improvement in groundnut (Arachis hypogaea L.) against major biotic and abiotic stress factors 花生(arachhis hypogaea L.)抗主要生物和非生物胁迫因子遗传改良的转基因途径

IF 3.5

Journal of Genetic Engineering and Biotechnology Pub Date : 2018-12-01 DOI: 10.1016/j.jgeb.2018.08.005

Saikat Gantait , Suvendu Mondal

{"title":"Transgenic approaches for genetic improvement in groundnut (Arachis hypogaea L.) against major biotic and abiotic stress factors","authors":"Saikat Gantait , Suvendu Mondal","doi":"10.1016/j.jgeb.2018.08.005","DOIUrl":"10.1016/j.jgeb.2018.08.005","url":null,"abstract":"<div><p>Cultivated groundnut (<em>Arachis hypogaea</em> L.) is considered as one of the primary oilseed crops and a major fodder for cattle industry in most of the developing countries, owing to its rich source of protein. It is due to its geocarpic nature of growth that the overall yield performance of groundnut is hindered by several biotic and abiotic stress factors. Multidimensional attempts were undertaken to combat these factors by developing superior groundnut varieties, modified with integral mechanism of tolerance/resistance; however this approach proved to be futile, owing to inferior pod and kernel quality. As a superior alternative, biotechnological intervention like transformation of foreign genes, either directly (biolistic) or via <em>Agrobacterium</em>, significantly aided in the development of advanced groundnut genotypes equipped with integral resistance against stresses and enhanced yield attributing traits. Several genes triggered by biotic and abiotic stresses, were detected and some of them were cloned and transformed as major parts of transgenic programmes. Application of modern molecular biological techniques, in designing biotic and abiotic stress tolerant/resistant groundnut varieties that exhibited mechanisms of resistance, relied on the expression of specific genes associated to particular stress. The genetically transformed stress tolerant groundnut varieties possess the potential to be employed as donor parents in traditional breeding programmes for developing varieties that are resilient to fungal, bacterial, and viral diseases, as well as to draught and salinity. The present review emphasizes on the retrospect and prospect of genetic transformation tools, implemented for the enhancement of groundnut varieties against key biotic and abiotic stress factors.</p></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jgeb.2018.08.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36985828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Expression, purification and biological characterisation of recombinant human irisin (12.5 kDa) 重组人鸢尾素(12.5 kDa)的表达、纯化及生物学特性研究

IF 3.5

Journal of Genetic Engineering and Biotechnology Pub Date : 2018-12-01 DOI: 10.1016/j.jgeb.2018.06.007

Kalpana Panati , Venkata Ramireddy Narala , Vydyanath R. Narasimha , Madhavi Derangula , Venkat R.R. Arva Tatireddigari , Suneetha Yeguvapalli

{"title":"Expression, purification and biological characterisation of recombinant human irisin (12.5 kDa)","authors":"Kalpana Panati , Venkata Ramireddy Narala , Vydyanath R. Narasimha , Madhavi Derangula , Venkat R.R. Arva Tatireddigari , Suneetha Yeguvapalli","doi":"10.1016/j.jgeb.2018.06.007","DOIUrl":"10.1016/j.jgeb.2018.06.007","url":null,"abstract":"<div><p>Fibronectin type III domain containing 5 (FNDC5) is a transmembrane protein. Upon cleavage, it yields a peptide called irisin that is supposedly bind to an unknown receptor and facilitates browning of white adipose tissue (WAT). Increased levels of irisin are associated with increased levels of energy expenditure markers PGC-1α, UCP-1, besides abundance of beige adipocytes in WAT. Though varied sizes of irisin were reported in humans and rodents it is not yet clear about the actual size of the irisin produced physiologically. Hence, we cloned and expressed human irisin (32–143 aa of FNDC5) in <em>Escherichia coli</em> based on the proposed cleavage site that yields 12.5 kDa peptide to study its antigenicity and other biological functions <em>in vitro</em>. We purified recombinant human irisin (rh-irisin) to 95% homogeneity with simple purification method with a yield of 25 mg/g wet cell pellet. rh-irisin has been detected by commercially available antibodies from different sources with similar antigenicity. Biological activity of the rh-irisin was confirmed by using 3T3-L1 pre-adipocyte differentiation by Oil red O staining. Further, rh-irisin treatment on pre-adipocytes showed increased expression of markers associated with energy expenditure. As it is involved in energy expenditure process, it could be considered as potential therapeutic option for various metabolic diseases.</p></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jgeb.2018.06.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36940006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Micropropagation protocol for Antigonon leptopus an important ornamental and medicinal plant 一种重要的观赏和药用植物——钩藤的微繁技术

IF 3.5

Journal of Genetic Engineering and Biotechnology Pub Date : 2018-12-01 DOI: 10.1016/j.jgeb.2018.03.008

Zenna Fawzia Ghareeb , Lobna S. Taha

{"title":"Micropropagation protocol for Antigonon leptopus an important ornamental and medicinal plant","authors":"Zenna Fawzia Ghareeb , Lobna S. Taha","doi":"10.1016/j.jgeb.2018.03.008","DOIUrl":"10.1016/j.jgeb.2018.03.008","url":null,"abstract":"<div><p>The effect of some factors on <em>in vitro</em> consecutive micropropagation behavior of <em>Antigonon leptopus</em> was examined including those of culture establishment, shootlets multiplication, rooting and acclimatization stages. The highest percent of aseptic cultures and survival of explants (100%) were obtained as a result of using Clorox 10% for 3 min followed by MC 0.1% for 2 min while, using each of them individually (Clorox 20% or MC 0.1%) for 5 min caused the highest percent of shoot formation. During the multiplication stage, the highest percent of shoot formation was reached to 100% with repeating culture of explants (two times) on MS medium supplemented with 2ip at 1.0 and IBA at 0.2 mg/l. The highest numbers of shootlets/explant were obtained when 2.0 mg/l of BAP or 0.5 mg/l BA + 0.2 mg/l of IBA were added to MS culture medium. Culturing the explants on MS medium supplemented with 2ip at 0.5 or 1.0 mg/l each combined with 0.2 mg/l of IBA showed the longest shootlets. Reducing the strength of culture media to ½ or ¾ had promotion effect on rooting formation of shootlets. The best results of plant acclimatization (survival percent, plant height and root length) were obtained by using sand or peat moss soil. The amplified DNA fragments using B7, B9 and C19 primers for mother and micropropagated plants showed that the produced pattern by primer B7 had a maximum number of 10 bands of DNA fragments with molecular size ranging between 1025.57 and 176.36 bp, micropropagated plants showed 95.2% similarity in relation to mother plant.</p></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jgeb.2018.03.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36941367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Partial purification and characterization of exoinulinase produced from Bacillus sp. 芽孢杆菌产外菊粉酶的部分纯化及特性研究。

IF 3.5

Journal of Genetic Engineering and Biotechnology Pub Date : 2018-12-01 DOI: 10.1016/j.jgeb.2018.03.001

R. Ramapriya, A. Thirumurugan, T. Sathishkumar, D.R. Manimaran

{"title":"Partial purification and characterization of exoinulinase produced from Bacillus sp.","authors":"R. Ramapriya, A. Thirumurugan, T. Sathishkumar, D.R. Manimaran","doi":"10.1016/j.jgeb.2018.03.001","DOIUrl":"10.1016/j.jgeb.2018.03.001","url":null,"abstract":"<div><p>Inulinase are industrial food enzymes which have gained much attention in recent scenario. In this study, Inulinase producing eight bacterial colonies were isolated and screened from three different plant root tubers soil sample. Among 8 inulinase producing colonies, the higher yielding colony was selected with 25.10 U/mL for further studies. The best inulinase producing colony was identified by partial 16S rRNA gene sequence as <em>Bacillus</em> sp. The crude inulinase was purified by using ammonium sulphate precipitation, dialysis and ion exchange chromatography on DEAE – sephacel and obtained 1.9 purification fold with total activity 293 U. The purified enzyme was subjected to characterization studies and it was found to be stable at 30–60 °C and optimum temperature was at 55 °C. The enzyme was stable at pH 3.0–7.0 and optimum pH was at 6.5. The K<sub>m</sub> and V<sub>max</sub> value for inulinase was found to be 0.117 mg/mL and 4.45 μmol min mg<sup>−1</sup> respectively, demonstrate its greater affinity. Hence, this enzyme can be widely used for the production of fructose, and fructooligosaccharides, which are important ingredients in food and pharmaceutical industry.</p></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jgeb.2018.03.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36541821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Valorisation of chicken feathers for xanthan gum production using Xanthomonas campestris MO-03 利用黄单胞菌MO-03生产黄原胶用鸡毛的研究

IF 3.5

Journal of Genetic Engineering and Biotechnology Pub Date : 2018-12-01 DOI: 10.1016/j.jgeb.2018.07.005

Murat Ozdal, Esabi Basaran Kurbanoglu

{"title":"Valorisation of chicken feathers for xanthan gum production using Xanthomonas campestris MO-03","authors":"Murat Ozdal, Esabi Basaran Kurbanoglu","doi":"10.1016/j.jgeb.2018.07.005","DOIUrl":"10.1016/j.jgeb.2018.07.005","url":null,"abstract":"<div><p>Xanthan gum is an important commercial polysaccharide produced by <em>Xanthomonas</em> species. In this study, xanthan production was investigated using a local isolate of <em>Xanthomonas campestris</em> MO-03 in medium containing various concentrations of chicken feather peptone (CFP) as an enhancer substrate. CFP was produced with a chemical process and its chemical composition was determined. The addition of CFP (1–8 g/l) increased the conversion of sugar to xanthan gum in comparison with the control medium, which did not contain additional supplements. The highest xanthan production (24.45 g/l) was found at the 6 g/l CFP containing control medium in 54 h. This value was 1.73 fold higher than that of control medium (14.12 g/l). Moreover, addition of CFP improved the composition of xanthan gum; the pyruvate content of xanthan was 3.86% (w/w), higher than that of the control (2.2%, w/w). The xanthan gum yield was also influenced by the type of organic nitrogen sources. As a conclusion, CFP was found to be a suitable substrate for xanthan gum production.</p></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jgeb.2018.07.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36930314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

In silico analysis of squalene synthase in Fabaceae family using bioinformatics tools 豆科植物角鲨烯合成酶的生物信息学分析

IF 3.5

Journal of Genetic Engineering and Biotechnology Pub Date : 2018-12-01 DOI: 10.1016/j.jgeb.2018.06.001

Zahra Aminfar , Masoud Tohidfar

{"title":"In silico analysis of squalene synthase in Fabaceae family using bioinformatics tools","authors":"Zahra Aminfar , Masoud Tohidfar","doi":"10.1016/j.jgeb.2018.06.001","DOIUrl":"10.1016/j.jgeb.2018.06.001","url":null,"abstract":"<div><p>Triterpenoid saponins are a diverse group of bioactive compounds, which are used for possessing of many biomedical and pharmaceutical products. Generally, squalene synthase (SQS) is defined as an emerging and essential branch point enzyme far from the major pathway of isoprenoids biosynthetic and a latent adjusting point, which manages carbon flux into triterpenes biosynthesis and sterols. The present study deals with the detailed characterization of SQS by bioinformatics approaches to evaluate physicochemical properties, structural characteristics including secondary and 3D structure prediction and functional analysis from eight plants related to Fabaceae family and <em>Arabidopsis thaliana</em>. Bioinformatics analysis revealed that SQS proteins have two transmembrane regions in the C-terminal. The predicted motifs were used to design universal degenerate primers for PCR analysis and other molecular applications. Phylogenetic analysis showed conserved regions at different stretches with maximum homology in amino acid residues within all SQSs. The secondary structure prediction results showed that the amino acid sequence of all squalene synthases had α helix and random coil as the main components. The reliability of the received model was confirmed using the ProSA and RAMPAGE programs. Determining of active site by CASTp proposes the possibility of using this protein as probable medication target. The findings of the present study may be useful for further assessments on characterization and cloning of squalene synthase.</p></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jgeb.2018.06.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36930899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

High level expression and purification of recombinant flounder growth hormone in E. coli 重组牙鲆生长激素在大肠杆菌中的高水平表达和纯化

IF 3.5

Journal of Genetic Engineering and Biotechnology Pub Date : 2018-12-01 DOI: 10.1016/j.jgeb.2018.03.006

Tae-Jin Choi , Temesgen Tola Geletu

{"title":"High level expression and purification of recombinant flounder growth hormone in E. coli","authors":"Tae-Jin Choi , Temesgen Tola Geletu","doi":"10.1016/j.jgeb.2018.03.006","DOIUrl":"https://doi.org/10.1016/j.jgeb.2018.03.006","url":null,"abstract":"<div><p>Recombinant flounder growth hormone was overproduced in <em>E. coli</em> by using codon optimized synthetic gene and optimized expression conditions for high level production. The gene was cloned into PET-28a expression vector and transformed into <em>E. coli</em> BL21 (DE3). Induction at lower temperature, lower IPTG concentrations and richer growth media during expression resulted in increased expression level. The protein expression profile was analyzed by SDS-PAGE, the authenticity was confirmed by western blotting and the concentration was determined by Bradford assay. In addition, several attempts were made to produce soluble product and all resulted in insoluble product. The overexpressed protein was efficiently purified from inclusion bodies by moderate speed centrifugation after cell lysis. Among the solubilization buffers examined, buffer with 1% N-lauroylsarcosine in the presence of reducing agent DTT at alkaline pH resulted in efficient solubilization and recovery. The denaturant was removed by filtration and dialysis. The amount of the growth hormone recovered was significantly higher than previous reports that expressed native growth hormone genes in <em>E. coli</em>. The methodology adapted in this study, can be used to produce flounder growth hormone at large scale level so that it can be used in aquaculture. This approach may also apply to other proteins if high level expression and efficient purification is sought in <em>E. coli</em>.</p></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jgeb.2018.03.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92076098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Biolytic extraction of poly(3-hydroxybutyrate) from Bacillus megaterium Ti3 using the lytic enzyme of Streptomyces albus Tia1 利用白色链霉菌Tia1酶水解从巨型芽孢杆菌Ti3中提取聚(3-羟基丁酸)

IF 3.5

Journal of Genetic Engineering and Biotechnology Pub Date : 2018-12-01 DOI: 10.1016/j.jgeb.2018.07.004

Neetu Israni, Surabhi Thapa, Srividya Shivakumar

{"title":"Biolytic extraction of poly(3-hydroxybutyrate) from Bacillus megaterium Ti3 using the lytic enzyme of Streptomyces albus Tia1","authors":"Neetu Israni, Surabhi Thapa, Srividya Shivakumar","doi":"10.1016/j.jgeb.2018.07.004","DOIUrl":"https://doi.org/10.1016/j.jgeb.2018.07.004","url":null,"abstract":"<div><p>The applicability of <em>Streptomyces</em> sp. cell lytic enzymes for devising a simple and competent biological polyhydroxyalkanoate (PHA) recovery approach from <em>Bacillus megaterium</em> cells was investigated. <em>B. megaterium</em> strain Ti3 produced 50% (w/w) PHA using glucose as carbon source. The intracellular PHA was recovered employing a non-PHA accumulating actinomycetes (Tia1) identified as <em>Streptomyces albus</em>, having potent lytic activity against living and heat inactivated <em>B. megaterium</em>. Interestingly, maximum biomass (2.53 ± 0.6 g/L by 24 h) of the lytic actinomycete was obtained in PHA production medium itself thus circumventing the prior actinomycete acclimatization just by co-inoculation with <em>B. megaterium</em> as an inducer. Maximum lytic activity was observed at pH 6.0, 40 °C, 220 mg of biomass and 33.3 mL of concentrated culture filtrate in a 100 mL reaction mixture. Preliminary biochemical investigations confirmed the proteolytic and caseinolytic nature of the lytic enzyme. PHA yield of 0.55 g/g by co-inoculation extraction approach was comparable with the conventional sodium hypochlorite based extraction method. Interestingly, <em>S. albus</em> also demonstrated a broad spectrum lytic potential against varied Gram-negative and Gram-positive PHA producers highlighting the extensive applicability of this biolytic PHA recovery approach. The lytic enzyme retained almost 100% relative activity on storage at −20 °C upto two months. <sup>1</sup>H Nuclear magnetic resonance analysis of the extracted polymer confirmed it as a homopolymer composed of 3-hydroxybutyrate monomeric units. This is the first report on <em>Streptomyces</em> sp. based biological and eco-friendly, intracellular PHA recovery from <em>Bacillus</em> spp.</p></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jgeb.2018.07.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91979800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19