Journal of Genetic Engineering and Biotechnology最新文献

{"title":"Enhanced carotenoid accumulation in Chloroccocum humicola under controlled CO2 and light conditions","authors":"Chatchai Kunyawut,&nbsp;Idtisak Paopo,&nbsp;Chakkrit Umpuch","doi":"10.1016/j.jgeb.2025.100619","DOIUrl":"10.1016/j.jgeb.2025.100619","url":null,"abstract":"<div><div>Carotenoids are potent antioxidants and high-value bioactive compounds that green microalgae can efficiently synthesize. This study aimed to enhance carotenoid production in <em>Chlorococcum humicola</em> TISTR 8551 using a two-stage cultivation strategy in a 10-L air-lift photobioreactor (ALPBR), separating biomass accumulation and stress induction phases. During the “green stage” (9 days), cells were grown in modified BG-11 medium (N:P ratio 31:1) under 3,500 Lux white LED light with varying CO₂ concentrations (1–3 % v/v) to maximize biomass yield. In the subsequent “red stage” (15 days), environmental stressors, including elevated salinity and intensified light exposure, were applied to stimulate carotenoid biosynthesis. The highest total carotenoid content (38.72 ± 1.04 mg/L, 0.313 ± 0.018 mg/g biomass) was observed under 3 % CO₂ supplementation, likely due to enhanced photosynthetic carbon fixation and improved precursor availability via glucose metabolism. An optimal white light intensity of 25,000 Lux produced 32.03 ± 1.52 mg/L carotenoids. Additionally, the combination of 100,000 Lux white light with 1,600 Lux blue light significantly increased β-carotene content (6.98 ± 0.28 % of total carotenoids), while 2,400 Lux blue light yielded the highest astaxanthin level (5.01 ± 0.18 % of total carotenoids). These results highlight the synergistic effects of CO₂ enrichment<strong>,</strong> spectral light modulation<strong>,</strong> and stage-specific stress application in promoting targeted carotenoid biosynthesis. This study offers practical insights for optimizing large-scale microalgal pigment production in controlled photobioreactor systems.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100619"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics analysis unveils the complex interplay between diabetes and hypertension in regulating renal cell carcinoma pathway followed by pancreatic metastasis","authors":"K.M. Tanjida Islam ,&nbsp;Roksana Khanam ,&nbsp;Aninda Roy ,&nbsp;Ramisa Binti Mohiuddin ,&nbsp;Jannati Akter ,&nbsp;Samia Haque ,&nbsp;Sheikh Abdullah Al Ashik ,&nbsp;Saborni Sarker ,&nbsp;A.K.M. Mohiuddin ,&nbsp;Shahin Mahmud","doi":"10.1016/j.jgeb.2025.100616","DOIUrl":"10.1016/j.jgeb.2025.100616","url":null,"abstract":"<div><div>Renal cell carcinoma (RCC) is often associated with metabolic disorders such as type 2 diabetes mellitus (T2DM) and hypertension. While existing research has established connections between these metabolic conditions and RCC, the underlying mechanisms driving RCC followed by pancreatic metastasis remain incompletely understood. Therefore, our study aimed to investigate the complex interplay between metabolic disorders (type 2 diabetes and hypertension) and malignancies (renal cell carcinoma and pancreatic cancer). To investigate the hidden link, we performed an integrative transcriptomic analysis. The analysis focuses only on T2DM and hypertension to identify a connection with the RCC pathway. Our analysis revealed that 190 significantly upregulated genes, of which MET emerged as a master regulator in RCC, while KRAS was the key regulator in pancreatic cancer. Furthermore, we identified key microRNAs (has-mir-1-3p, has-mir-16-5p, and has-mir-455-3p) and transcription factors (MBD1, TFDP1, and KLF9) regulate these targets. Additionally, we identified and validated CDC42, PTPN11, TGFB3, and MET as potential prognostic or theragnostic biomarkers. MET, KRAS, and PIK3CD emerged as the most promising therapeutic targets against a panel of 28 repurposable inhibitory drugs. The genetic and immune association suggested that CD8 + T cells are the key immune infiltrate significantly associated with poor survival outcomes in RCC and pancreatic cancer patients. Mutational analysis further highlighted the significance of KRAS G12C, G12V, and G12D mutations, which were common between RCC and pancreatic metastasis. Our study provides critical insights into the statistically significant associations between metabolic disorders and malignancies, emphasizing the potential of tailored therapies alongside shared therapies in managing RCC and its progression to pancreatic metastasis.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100616"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatics-driven identification of pathogenic missense nsSNPs in the human proto-oncogene SRC and cancer susceptibility","authors":"Md. Shakil Ahamed,&nbsp;Roksana Khanam,&nbsp;K.M. Tanjida Islam,&nbsp;Fahmida Tabassum,&nbsp;Md. Al Amin,&nbsp;Jannatul Fardous,&nbsp;Nadira Hoque Tashpie,&nbsp;A.K.M. Mohiuddin,&nbsp;Shahin Mahmud","doi":"10.1016/j.jgeb.2025.100618","DOIUrl":"10.1016/j.jgeb.2025.100618","url":null,"abstract":"<div><div>SRC is a proto-oncogene that regulates cell proliferation and survival, and its dysregulation is commonly observed in diverse cancers. While SRC kinase dysregulation is well-established as a cancer driver, the functional consequences of its genetic variants, particularly non-synonymous single-nucleotide polymorphisms (nsSNPs) are not fully understood. Therefore, we employed an integrative computational approach to identify nsSNPs in SRC and analyze their impact on protein function and structure. Out of the 512 missense nsSNPs analyzed, 42 were predicted to be deleterious, with 12 likely to destabilize protein structure. Among these, three mutations, namely W151C (rs746439256), Y419N (rs2147125119), and P465S (rs1251532695), were particularly significant, causing substantial physicochemical changes. Molecular dynamics simulations revealed that these variations reduce protein stability and flexibility, resulting in conformational alterations. Docking study demonstrated that these mutations disrupt the binding interface residues of the SRC-FAK complex and affect dasatinib binding affinity. Additionally, gene expression analysis linked mutated SRC to dysregulation of cancer-related genes, especially in multiple myeloma and uterine cancer, and suggested reciprocal regulation by other mutated genes across malignancies. These findings highlight the oncogenic potential of SRC mutations and pave the way for future population-based studies exploring their role as diagnostic biomarkers, therapeutic targets, and modulators of drug response in personalized cancer treatment.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100618"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of peroxidase from potato leaves Solanum tuberosum: Application in glucose diagnostic kit","authors":"Hassan M.M. Masoud ,&nbsp;Mohammed M. Abdel-Monsef ,&nbsp;Mohamed S. Helmy ,&nbsp;Sayed S. Esa ,&nbsp;Doaa A. Darwish","doi":"10.1016/j.jgeb.2025.100584","DOIUrl":"10.1016/j.jgeb.2025.100584","url":null,"abstract":"<div><div>Peroxidases play a pivotal role in many medical applications such as diagnostic kits and ELISA assays. This study reports the purification and biochemical characterization of peroxidase from potato leaves (PLPOD) and its application in the formulation of a glucose diagnostic kit. PLPOD was purified through CM-cellulose ion-exchange and Sephacryl S-300 gel filtration chromatography, achieving an 11.8-fold purification with 48 % recovery and a final specific activity of 705.7 U/mg. Native PAGE and activity staining confirmed the enzyme’s purity and homogeneity. PLPOD molecular weight was estimated from gel filtration column as 64 kDa, but on SDS gel, there were three PLPOD isoforms of approximated molecular weights ranging from ∼ 40–60 kDa. PLPOD exhibited optimal activity at pH 5.2, with Zn²⁺ and Ni²⁺ enhancing activity, while Ca²⁺ and Fe²⁺ inhibited it. Inhibitor analysis confirmed the heme-dependent nature of the enzyme. The <em>K_m</em> values for guaiacol and H₂O₂ were 0.067 mM and 40 mM, respectively, consistent with typical plant peroxidases. A glucose diagnostic kit developed using PLPOD showed strong concordance with a commercial glucose kit when tested on normal and diabetic serum samples demonstrating its clinical applicability. These findings suggest that PLPOD is a viable cost-effective for use in diagnostic assays and kits.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100584"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and evolutionary analysis of SUT genes reveals key regulators of drought stress response in finger millet (Eleusine coracana)","authors":"Kasinathan Rakkammal ,&nbsp;Pandiyan Muthuramalingam ,&nbsp;Hyunsuk Shin ,&nbsp;Manikandan Ramesh","doi":"10.1016/j.jgeb.2025.100592","DOIUrl":"10.1016/j.jgeb.2025.100592","url":null,"abstract":"<div><div>Sucrose transporters (SUTs) mediate sucrose movement across plant membranes, playing a crucial role in carbon allocation and stress responses. Although finger millet (<em>Eleusine coracana</em>) is known for its inherent drought resistance, the specific involvement of <em>SUT</em> genes in this characteristic is still unclear. This study aimed to identify the <em>SUT</em> genes of millet and to assess their expression in drought conditions. Five <em>SUT</em> genes (<em>EcSUT1</em>-<em>EcSUT5</em>) were identified that encode proteins with 9–12 transmembrane domains. Phylogenetic analysis clustered these SUT members across all three main SUT groups, suggesting an evolutionary divergence within the family. Synteny analysis revealed conserved genomic regions, with <em>EcSUT2</em> showing 91–94% identity with orthologs in closely related grasses. Structural models further confirmed their typical transmembrane architecture. Interaction analysis identified EcSUT2 as a key interaction with SWEET transporters. Furthermore, the promoter regions of <em>EcSUT2</em> and <em>EcSUT5</em> were found to be enriched with hormone and stress-responsive elements. Under drought conditions, <em>EcSUT1-EcSUT4</em> displayed transient induction, while <em>EcSUT5</em> showed sustained upregulation, especially in the roots, notably after 48 h. The finger millet SUT family exhibits evolutionary conservation within grasses, with individual genes that play different roles in stress response. The persistent upregulation of <em>EcSUT5</em> under drought strongly suggests its involvement in maintaining sucrose transport during long-term adverse conditions. This candidate gene requires further functional validation to uncover the stress dynamics for sustainable crop improvement.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100592"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145332239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the functional significance of catalytic and chitin-binding domains for the anti-cancer property of a bacterial endochitinase","authors":"Ankita Shrivastava ,&nbsp;Manik Goel ,&nbsp;Md Fahim Khalid,&nbsp;Priyamedha Yadav,&nbsp;Suroj Maharjan,&nbsp;Rinkoo Devi Gupta","doi":"10.1016/j.jgeb.2025.100596","DOIUrl":"10.1016/j.jgeb.2025.100596","url":null,"abstract":"<div><div>Chitinases are enzymes that facilitate the breakdown of chitin and also interact with carbohydrate moieties such as heparin sulphate due to structural similarity with chitin, thereby influencing cell adherence and migration. Bacterial <em>endo</em>-chitinase contains several domains, such as a catalytic domain, an FNIII domain, and a chitin-binding domain. Recent studies have demonstrated the anti-cancer property of catalytically active chitinases; however, the mechanism and targets have not yet been explored. Therefore, this study investigates the importance of the catalytic and chitin-binding domains of a bacterial <em>endo</em>-chitinase (ChiC) for the anti-cancer property by creating domain-specific mutants. Initially, an <em>in silico</em> study identified an evolutionarily conserved tryptophan (W300) within the catalytic cleft of ChiC, which was subjected to site-directed mutagenesis to create an inactive ChiC mutant. Similarly, another ChiC mutant was created without the chitin-binding domain. The recombinantly expressed WT and mutant ChiC proteins were utilized to analyze their effects on the cell viability, cell cycle, and migratory and invasive behaviour of MCF-7 cells. The mutants of ChiC, lacking either catalytic property or chitin-binding domain, resulted in a loss of their ability to inhibit cell proliferation and migration. These observations suggest that the catalytic and chitin-binding domains are essential for exhibiting anti-proliferative and anti-migratory effects, providing insights for future therapeutic applications.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100596"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145332236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic characterization and proteomic profiling of venoms from the medically important Androctonus species","authors":"Alhussin M.A. Megaly ,&nbsp;Masahiro Miyashita ,&nbsp;Abdulaziz R. Alqahtani ,&nbsp;Mohammed Abdel-Wahab","doi":"10.1016/j.jgeb.2025.100566","DOIUrl":"10.1016/j.jgeb.2025.100566","url":null,"abstract":"<div><div>Egyptian scorpions of the <em>Androctonus</em> genus (family Buthidae) produce life-threatening stings owing to their neurotoxic venom. However, the composition and enzymatic activities of their venoms remain poorly understood: We used electrophoresis to analyze the protein components of venoms collected from three <em>Androctonus</em> species: <em>Androctonus amoreuxi</em>, <em>Androctonus australis</em>, and <em>Androctonus bicolor</em>. Mass spectrometric analysis was performed to characterize the peptides present in these venoms. The phospholipase A₂ (PLA₂), hyaluronidase, and protease activities of the venoms were examined to gauge their potential contribution to venom toxicity. Finally, the antibacterial and hemolytic activities of the venoms were evaluated. The electrophoretic profiles of the three venoms showed features specific to each species, with distinct protein bands observed at 75, 74, 67, 48, 46, 40, and 28 kDa, along with a notable band above the 15-kDa mark. Liquid chromatography/mass spectrometry analyses were used to detect the presence of 369, 324, and 351 components in with molecular masses in the range of 500–10,000 Da in the venoms of <em>A. amoreuxi</em>, <em>A. australis</em>, and <em>A. bicolor</em>, respectively. Disulfide-rich peptides (three disulfide bridges) were abundant, but peptides without disulfide bonds were also detected in all venom samples. All three venoms exhibited hyaluronidase activities, whereas protease and PLA₂ activities were either weak (at 1 µg and 10 µg) or undetectable, even at higher concentrations (up to 20 µg). All assays were performed using venoms standardized by dry weight to ensure consistent protein quantities. Crude venoms of <em>A. amoreuxi</em> and <em>A. australis</em> showed antibacterial activity against <em>E. coli</em> and <em>B. subtilis</em> (5–10 μg), whereas <em>A. bicolor</em> required 10 μg. Hydrophobic fractions (40–55 min) of <em>A. australis</em> alone retained this activity. This work furthers our knowledge of the enzymatic and peptide composition of <em>Androctonus</em> venoms, unveiling their potential in drug delivery enhancement and other biomedical applications. These findings will inform the development of better strategies for the treatment and prevention of scorpion envenomation.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100566"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive examination of demographic, psychological, cognitive, biochemical, and genetic profiles of methamphetamine addicts","authors":"Haider K. Hussain ,&nbsp;Yolanda Loarce Tejada ,&nbsp;Anna Barbaro","doi":"10.1016/j.jgeb.2025.100564","DOIUrl":"10.1016/j.jgeb.2025.100564","url":null,"abstract":"<div><div>Methamphetamine (MA) addiction is a serious public health concern with wide-ranging neurobiological and behavioral effects. This study aimed to assess the demographic, psychological, cognitive, biochemical, and genetic profiles of individuals with methamphetamine dependence, focusing on neurotransmitter levels and the expression of addiction- and aggression-related genes. Sixty male methamphetamine users and thirty age-matched healthy controls were recruited. Participants underwent psychological assessments, cognitive testing, and biochemical evaluation of serotonin and dopamine levels using ELISA. Gene expression of SLC6A4 and COMT was quantified via real-time PCR. Significant alterations were observed in the methamphetamine group compared to controls, including reduced serotonin (17.1 ± 3.1 vs. 20.5 ± 3.2 ng/mL; p = 0.002) and dopamine levels (46.3 ± 7.2 vs. 52.4 ± 6.5 ng/mL; p = 0.015), as well as down-regulation of SLC6A4 (0.64-fold vs. 1.00; p = 0.001) and up-regulation of COMT (1.47-fold vs. 1.00; p = 0.028). These biochemical and genetic changes were correlated with increased aggression and cognitive impairments. The findings underscore the impact of prolonged MA use on neurochemical balance and gene expression, contributing to the development of aggressive behaviors and addictive patterns. Tailored treatment strategies that integrate genetic and psychological profiling, along with longitudinal monitoring, are essential to address the multifactorial nature of methamphetamine addiction and improve clinical outcomes.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100564"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of salinity tolerance of Egyptian barley genotypes and their dehydrin 6-based single nucleotide polymorphisms (SNPs) diversity","authors":"Reda M. Gaafar ,&nbsp;Ismael A. Khatab ,&nbsp;Samah A. Mariey","doi":"10.1016/j.jgeb.2025.100614","DOIUrl":"10.1016/j.jgeb.2025.100614","url":null,"abstract":"<div><div>In many countries, freshwater sources for agricultural irrigation are scarce, making it challenging to meet the food production needs of the growing human population. Utilizing seawater for agriculture could be a potential solution for limited water resources. A lysimeter experiments evaluated fifteen barley genotypes irrigated with different levels of diluted seawater (S1 = 4.0, S2 = 8.0, and S3 = 12.0 dSm⁻¹). Morpho-physiological traits and exploring single nucleotide polymorphism (SNP) diversity among the most tolerant and sensitive genotypes during the winter seasons (2019/2020 and 2020/2021) were examined. Irrigation with diluted seawater at ECw 12.0 dSm⁻¹ significantly decreased leaf area index (by 35.54 %), total chlorophyll content (by 18.97 %), chlorophyll fluorescence (by 46.36 %), plant height (by 29.17 %), number of tillers per square meter (by 43.73 %), number of grains per spike (by 30.56 %), thousand-kernel weight (by 36.07 %), and grain yield (by 34.27 %). In contrast, early flowering was increased by 21.67 %. The Dehydrin 6 gene (<em>Dhn6</em>) partial sequences, 770 bp long, were blasted and used to detect SNPs associated with salinity among genotypes. Several SNPs were identified, with 26 variable sites when aligning the partial <em>Dhn6</em> sequences. Eleven SNPs were identified between the salt-tolerant Giza 137 and salt-sensitive Giza 132 genotypes, all located in exonic regions. These results indicate a potential role in salt tolerance for cultivar Giza 137, and the SNP markers effectively differentiated barley genotypes, which could be useful in salinity breeding programs aimed at developing salinity-tolerant barley and addressing climate change.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100614"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145528326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotyping analysis of rice cv. Nipponbare overexpressing OsMADS56 gene in drought tolerance, plant height, and yield components","authors":"Dini Nurdiani,&nbsp;Dwi Widyajayantie,&nbsp;Enung Sri Mulyaningsih,&nbsp;Amy Estiati,&nbsp;Satya Nugroho","doi":"10.1016/j.jgeb.2025.100570","DOIUrl":"10.1016/j.jgeb.2025.100570","url":null,"abstract":"<div><h3>Background</h3><div>The MADS-box protein family is a transcription factor (TF) family whose members harbour a MADS-box domain located in their N-terminal. This domain consists of 60 amino acids and functions in DNA binding. In plants, these family genes broadly regulate plant growth, development and responses to various environmental stresses. In this study, an <em>OsMADS56</em> (702 bp) was isolated from rice (<em>Oryza sativa</em> cv. Nipponbare) to investigate its role in response to drought stress and other agronomic phenotypes at both the vegetative and generative stages.</div></div><div><h3>Results</h3><div>Rice mutants harbouring overexpressed <em>OsMADS56</em> gene driven by the constitutive 35S promoter were generated. Quantitative real-time (qRT)-PCR confirmed the overexpression of <em>OsMADS56</em> at T1 (M76.7) generation. Observation of the T2 <em>OsMADS56</em>-overexpressed rice line (OX7) showed improved tolerance to drought stress. It also showed different agronomic phenotypes compared to wild type (WT), such as shorter plant height (PH) and improved productivity as indicated by earlier heading date (HD), a higher number of panicles (NP), a higher number of grains (NG) and higher grain yield (GY) than WT.</div></div><div><h3>Conclusion</h3><div>The findings suggest that <em>OsMADS56</em> was implicated in the rice response to PEG-induced drought stress, rice plant height, and yield components. The <em>OsMADS56</em> gene and the characterized line can be utilized in further studies on rice adaptation against abiotic stress and its productivity.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100570"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}