Journal of Genetic Engineering and Biotechnology最新文献

{"title":"Exploring the potential of Bacillus spp. for secondary metabolite production through genome mining approaches","authors":"Showti Raheel Naser,&nbsp;Sanjana Fatema Chowdhury,&nbsp;Md. Murshed Hasan Sarkar,&nbsp;Md. Ahashan Habib,&nbsp;Shahina Akter,&nbsp;Tanjina Akhtar Banu,&nbsp;Barna Goswami,&nbsp;Md. Salim Khan","doi":"10.1016/j.jgeb.2025.100595","DOIUrl":"10.1016/j.jgeb.2025.100595","url":null,"abstract":"<div><div>With the rise of antibiotic resistance along with the increase in the emergence of infectious diseases, the search for new therapeutic agents <u>is</u> of dire need. <em>Bacillus</em> is one of the widely distributed genera of bacteria, which has immense applications in biotechnology, medicine, and environmental protection, and is known to produce a vast range of secondary metabolites (SMs). Using several bioinformatics programming approaches along with phylogenetic and genomic comparisons, biosynthetic gene clusters (BGCs) were characterized from <em>Bacillus</em> reference genome sequences publicly available in databases to obtain new insights into the diversity and distribution of these BGCs in <em>Bacillus</em> for the discovery of unexplored SMs. From this study, average nucleotide identity (ANI) analysis of 87 species shows more than 70% similarities among them, whereas phylogenetic studies show species diversification into 4 clades based on the presence of 1–2 BGC types. High diversity for SMs production was observed in most of the species, where the majority of BGCs types were found to be terpene, nonribosomal peptide synthase (NRPS), polyketide synthase (PKS), hybrid gene clusters, ribosomally synthesized and post translationally modified peptide (RiPP) encoding for different characterized secondary metabolites, along with some unique metabolites characterized based on similarity confidence value. Many uncharacterized BGCs were found distributed within each species, giving new insights into novel compound discovery. These findings will open the door to the identification of species-specific pathways for SM development and contribute to creating new insights into therapeutic systems.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100595"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145362233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic differentiation and conservation insights into Salicornia iranica subsp. sinus-persica from Musa Bay using SCoT markers and DNA barcodes","authors":"Fatemeh Nasernakhaei ,&nbsp;Mahyar Zahraei","doi":"10.1016/j.jgeb.2025.100563","DOIUrl":"10.1016/j.jgeb.2025.100563","url":null,"abstract":"<div><div>Understanding genetic diversity is essential for the conservation and management of halophyte species, especially in ecologically sensitive and industrially impacted regions. In this study, 52 accessions of <em>Salicornia iranica</em> subsp. <em>sinus-persica</em> were collected from diverse microhabitats across Musa Bay, including coastal zones, islands, and petrochemical sites. Following comprehensive morphological assessments showing high phenotypic similarity, and to ensure broad ecological coverage, 16 accessions representing all major microhabitats were selected for molecular analysis. SCoT markers and DNA barcoding (ITS and <em>trnH-psbA</em> regions) were employed to investigate their genetic structure. The SCoT markers revealed considerable polymorphism and partial genetic differentiation, especially between accessions from petrochemical and natural saline habitats. ITS sequencing identified five haplotypes without forming a monophyletic clade, indicating shallow divergence and possible ancestral polymorphism. In contrast, no variation was detected in the plastid region <em>trnH-psbA</em>. These results represent the first global report of ITS and <em>trnH-psbA</em> haplotypes for this subspecies. While the findings are preliminary due to limited sample size and marker coverage, they provide a critical baseline for future studies employing high-resolution genomic tools. Our findings underscore the influence of habitat fragmentation and anthropogenic pressures on genetic patterns and emphasize the importance of conserving genetically distinct accessions in ecologically vulnerable habitats to support the long-term survival and sustainable utilization of this coastal halophyte.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100563"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-silico study on biomolecules derived from Cissus quadrangularis towards anti-inflammation","authors":"H. Nilofer Farjana ,&nbsp;G. Mohan Valiathan ,&nbsp;S. Mohanasatheesh","doi":"10.1016/j.jgeb.2025.100571","DOIUrl":"10.1016/j.jgeb.2025.100571","url":null,"abstract":"<div><h3>Objective</h3><div>CD 163 protein is a macrophage-specific protein and hemoglobin scavenger receptor. Elevated expression of CD 163 protein in macrophages is an important feature indicating that tissues are subjected to inflammation. It can be an effective diagnostic biomarker for inflammatory diseases like gingivitis and periodontitis. Cissus quadrangularis is used as a potent alternative medicine to control inflammation and heal fractured bones. It has high osteogenic potential by increasing osteoblast mineralization. Being a natural product with a wide range of benefits can lead to drug development for its high anti-inflammatory and anti-osteoporotic properties. Studies have shown that Cissus quadrangularis has significant anti-inflammatory potential and can be used as a potential target for therapeutic agents. The objective of the study is to explore the interaction of the phytonutrients derived from Cissus quadrangularis with CD 163 protein to establish the anti-inflammatory activity by in silico approach.</div></div><div><h3>Materials and methods</h3><div>The biomolecules derived from the plant Cissus quadrangularis namely β-Sitosterol, luteolin, piceatannol, quadrangualrin, resveratrol, and tannins were subjected to molecular docking using Autodock of 4.0 PyRx software.</div></div><div><h3>Results</h3><div>The study results showed drug-likeness and binding affinity between biologically active Cissus quadrangularis molecules against inflammation associated with CD 163 protein.</div></div><div><h3>Conclusion</h3><div>It can be concluded that the biomolecules derived from Cissus quadrangularis exhibited anti-inflammatory potential and thus it can be further explored by invitro analysis in developing a new drug.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100571"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of Canarium odontophyllum fruit phytoconstituents as potential candidates against epilepsy using in silico studies","authors":"Lim Joe Siang ,&nbsp;Kamini Vijeepallam ,&nbsp;Arunachalam Muthuraman ,&nbsp;Parasuraman Pavadai ,&nbsp;Thiruventhan Karunakaran ,&nbsp;Veerasamy Ravichandran","doi":"10.1016/j.jgeb.2025.100561","DOIUrl":"10.1016/j.jgeb.2025.100561","url":null,"abstract":"<div><div>Epilepsy is the third most common neurological illness worldwide, affecting over 65 million people. Current antiepileptic drugs (AEDs) are ineffective for about 30 % of epileptic patients. AED use is extremely expensive and associated with adverse effects such as ataxia, nystagmus, and central nervous system depression. Serious quality-of-life changes are another aspect of managing epileptic conditions. A variety of therapeutic plant components, including flavonoids, phenolics, terpenoids, and alkaloids, exhibit a wide range of biological actions, including antibacterial, antioxidant, antiulcer, antineoplastic, anti-inflammatory, antiepileptic, and immunostimulant properties. Because many of them are being researched in modern research to develop an active therapeutic candidate, we resorted to medicinal plants to investigate powerful antiepileptic components. So, in this in silico study, we aimed to identify potential antiepileptic agents from <em>Canarium odontophyllum</em> fruits using molecular docking, molecular dynamics, and ADMET prediction approach. In the present study, the virtual screening was performed on a set of 63 phytocompounds, identified from the hydroalcoholic (70 %) extract of <em>C. odontophyllum</em> fruit using LC-MS (LC-MS-QTOF) analysis, by using molecular docking simulation to examine their binding ability with seven proteins that are responsible for epilepsy. Further, the selected compounds with good binding affinity were subjected to ADMET screening to predict the pharmacokinetic properties, and molecular dynamics testing was done to confirm the outcomes. Our results revealed that <em>C. odontophyllum</em> phytoconstituents showed the strongest binding affinities against all tested enzymes. Moreover, kanzonol B could prominently interact with all tested proteins with moderate to good binding affinity (−6.70 to −10.65 kcal/mol). All top phytoconstituents obeyed Lipinski’s RO5 and drug-likeness properties. These computational studies suggested that the phytoconstituents of <em>C. odontophyllum</em> can serve as potent inhibitors of enzymes responsible for epilepsy. Thus, we recommend further experimental investigations to validate the promising results of the present in silico study.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100561"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144904237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global research trends and therapeutic potential of fibrinolytic enzymes","authors":"Chinmay Hazare,&nbsp;Prashant Bhagwat,&nbsp;Suren Singh,&nbsp;Santhosh Pillai","doi":"10.1016/j.jgeb.2025.100606","DOIUrl":"10.1016/j.jgeb.2025.100606","url":null,"abstract":"<div><div>Fibrinolytic enzymes are proteolytic enzymes that degrade fibrin clots. They play a significant role in managing thrombosis as they are crucial for the lysis of the clot. Despite the importance and extensive research on fibrinolytic enzymes, many have not been translated into therapeutics due to challenges such as immunogenicity, bleeding risks, and short half-life. Consequently, in this review, a comprehensive assessment was conducted to identify trends and gaps in fibrinolytic enzyme research, analyzing data spanning eight decades alongside important biotechnological discoveries, such as recombinant DNA technology and tissue plasminogen activators, to gain an in-depth understanding of this evolving research area. The field’s multidisciplinary nature encompasses medicine, biochemistry, genetics, molecular biology, and other related disciplines, thus highlighting its industrial potential. An international collaborative network involving 35 countries was identified, highlighting the need to diversify the collaborations to advance research on fibrinolytic enzymes. At the institutional level, South Korea leads in publications, whereas research in India and Japan is more decentralized. Keyword co-occurrence analysis identified thematic clusters and journals such as Thrombosis Research and the Journal of Microbiology and Biotechnology as the prominent publishers. Patent analysis points to Cordis Corporation and Bristol-Myers Squibb as key players. This comprehensive analysis provides insights into past and current trends, highlights gaps in fibrinolytic enzyme research, and sets the stage for future advancements in this field.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100606"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145424505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning and expression of outer membrane proteins from Vibrio alginolyticus, and evaluation of their immune response in Epinephelus coioides","authors":"Ngo Thi Huyen ,&nbsp;Dong Van Quyen ,&nbsp;Tran Nhu Quynh ,&nbsp;Le Thi Tuoi ,&nbsp;Vu Thi Bich Huyen","doi":"10.1016/j.jgeb.2025.100602","DOIUrl":"10.1016/j.jgeb.2025.100602","url":null,"abstract":"<div><div><em>Vibrio alginolyticus</em> and <em>Vibrio parahaemolyticus</em> pose significant threats to various marine fish species, with mortality rates reaching as high as 90 %. Outer membrane proteins represent promising antigens for creating immune responses. The bacterial strains <em>V. alginolyticus</em> A2 and <em>V. parahaemolyticus</em> N9 were isolated from diseased grouper samples collected in Vietnam. The two bacterial strains, <em>V. alginolyticus</em> A2 and <em>V. parahaemolyticus</em> N9, were identified as highly virulent, causing disease in <em>Epinephelus coioides</em>. This study assesses the protective efficacy of the recombinant proteins OmpK and OmpU, derived from the <em>ompK</em> and <em>ompU</em> genes of <em>V. alginolyticus</em> A2, in <em>E. coioides</em> against the <em>V. parahaemolyticus</em> N9 and <em>V. alginolyticus</em> A2 strains. The <em>ompK</em> and <em>ompU</em> genes were 798 bp and 1023 bp in length, respectively, encoding polypeptide chains of 265 and 340 amino acids. The amino acid sequences of OmpK and OmpU proteins demonstrated high conservation across the other <em>Vibrio</em> species. The relative percent survival (RPS) for the OmpK protein in orange-spotted groupers challenged with <em>V. alginolyticus</em> A2 and <em>V. parahaemolyticus</em> N9 was 89.18 % and 72.22 %, respectively. Similarly, the group of fish injected with OmpU protein exhibited the RPS values of 82.14 % and 61.11 % against <em>V. alginolyticus</em> A2 and <em>V. parahaemolyticus</em> N9, respectively. Both recombinant antigenic proteins, OmpK and OmpU, induced a strong immune response in <em>E. coioides</em> to <em>V. alginolyticus</em> A2. Most interestingly, OmpK could also cross-protect against <em>V. parahaemolyticus</em>. This result suggests that OmpU and OmpK protein could be used as potential candidates for vaccine development targeting <em>V. alginolyticus</em> and <em>V. parahaemolyticus</em>.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100602"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145424506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss-of-function variant of SLC30A8 rs13266634 (C > T) protects against type 2 diabetes by stabilizing ZnT8: Insights from epidemiological and computational analyses","authors":"Nilima Barman ,&nbsp;M Atiqul Haque ,&nbsp;Md Ridwan ,&nbsp;Debabrata Ghosh ,&nbsp;Abul B.M.M.K. Islam","doi":"10.1016/j.jgeb.2025.100565","DOIUrl":"10.1016/j.jgeb.2025.100565","url":null,"abstract":"<div><div>The loss-of-function variant rs13266634 (C &gt; T) in <em>SLC30A8</em> has been linked to type-2-diabetes (T2D) susceptibility with conflicting findings. We investigated its role among T2D patients of Bangladesh. The rs13266634 (C &gt; T) variant results in an arginine-to-tryptophan (p.R325W) substitution of <em>SLC30A8</em>-encoded zinc transporter protein, ZnT8. As zinc is crucial for insulin-crystallization for storage, we analyzed its wild-type (WZnT8) and variant (MZnT8) protein conformations via molecular-dynamics (MD) simulations. For the epidemiological analysis, a case-control study was conducted with 90 T2D patients from a diabetic-patient-cohort and 90 gender-matched healthy-controls. Genomic DNA was isolated from blood samples and genotyped by Sanger sequencing. MD simulations, spanning over 100 ns, were executed using GROMACS v2024.4. The homozygous wild CC-genotype was significantly more frequent in T2D patients (66.7 %) than in control (50.0 %) and was increasing T2D risk, with adjusted odds-ratio (OR_adj) of 2.25 under dominant model. Conversely, the polymorphic TT-genotype showed a protective effect, with an OR_adj of 0.196 (95 % CI:0.045–0.850, p = 0.029) in an additive model. MD simulations revealed that MZnT8 variant exhibited higher structural stability, lower flexibility and a more compact conformation than WZnT8. In conclusion, the polymorphic TT-genotype of <em>SLC30A8</em> plays a protective role in T2D susceptibility. Its protein variant, MZnT8, adopts a stable, compact conformation with lower flexibility, which may decrease zinc transport in insulin secreting granules and impair insulin crystallization. This structural adaptation could enhance glucose-stimulated insulin secretion and ultimately contribute to T2D protection.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100565"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silver nanoparticles synthesised using endophytic fungus Calonectria eucalypti from medicinal plant axifraga stolonifera and their bioactivity","authors":"Tianyu Lv ,&nbsp;Meiqi Chen ,&nbsp;Xixian Li ,&nbsp;Yu Long ,&nbsp;Yiwen Liang ,&nbsp;Tingting Jiang ,&nbsp;Jiayi Chen ,&nbsp;Yichao Huang ,&nbsp;Kailing Guo ,&nbsp;Yi Cheng ,&nbsp;Zujun Deng ,&nbsp;Zhuoya Wang","doi":"10.1016/j.jgeb.2025.100627","DOIUrl":"10.1016/j.jgeb.2025.100627","url":null,"abstract":"<div><div>Silver nanoparticles (AgNPs) are widely used but traditional synthesis methods are energy-intensive and polluting. Biosynthesis offers an eco-friendly approach, yet studies on medicinal plant endophytic fungi remain scarce. This work reports the isolation and characterization of AgNPs synthesized by endophytic fungi from <em>Saxifraga stolonifera</em>, along with an assessment of their bioactivities, including antimicrobial, anticancer, and antioxidant effects. AgNPs were biosynthesized using the aqueous extract of the fungus HECL10, which was identified as <em>Calonectria eucalypti</em>. The HECL10-AgNPs were characterised by UV‒vis spectroscopy, SEM, EDS, TEM, FT-IR and zeta potential analysis. The drug susceptibility experiments illustrated that HECL10-AgNPs exhibited potent antimicrobial activity against <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em>, <em>Staphylococcus aureus</em> and <em>Candida albicans</em>. Additionally, the cytotoxicity assays revealed that the HECL10-AgNPs exhibited prominent anticancer effects on the A549, A375, HeLa and HepG2 cell lines. Notably, the scavenging rates of HECL10-AgNPs at 200 µg/mL against DPPH, ABTS, and OH radicals were 90.49 %, 60.38 %, and 38.76 %, respectively. Compared to the H₂O₂-induced model group, the treatment of HECL10-AgNPs at concentrations of 25.00 µg/mL significantly reduced intracellular ROS levels in HaCaT cells by 44.8 %, and enhanced the activity of SOD, CAT, and GSH by 106.7 %, 2036.1 % and 43.5 %. The results demonstrated that HECL10-AgNPs possessed potent antioxidant properties. These findings revealed the potential for synthesising AgNPs using endophytic fungi from medicinal plants and emphasised the promise of HECL10-AgNPs as a multifunctional nanomedicine for biomedical applications.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100627"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145693134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly synthesis of silver nanoparticles from the leaf blades extract of seagrass Thalassia hemprichii: Multifunctional agents for biomedical and environmental applications","authors":"Shibin Eranhottu ,&nbsp;Tijo Cherian ,&nbsp;R. Mohanraju ,&nbsp;N. Sharmila Devi ,&nbsp;Jayalakshmi Sanal ,&nbsp;Lincy Sara Varghese ,&nbsp;P. Ambili ,&nbsp;Mini Thomas ,&nbsp;Fahmeeda Parveen P.S. ,&nbsp;Willie J.G.M. Peijnenburg","doi":"10.1016/j.jgeb.2025.100600","DOIUrl":"10.1016/j.jgeb.2025.100600","url":null,"abstract":"<div><div>The current study reports for the first time the synthesis of environmentally friendly silver nanoparticles (AgNPs) using leaf blades extract (THE) of seagrass <em>Thalassia hemprichii</em>. The AgNPs were described using several spectroscopic methods. The UV–visible spectral study of THE-AgNPs reported absorbance peak at 420 nm. The intrinsic stretching was seen in the FT-IR (Fourier transform infrared) spectrum inferring the presence and role of diverse functional species in the biological synthesis of AgNPs. The AgNPs reported uniform morphology, spherical shape and high stability validated by electron microscopic and zeta potential studies. The biosynthesized silver nanoparticles were employed in experiments including antibacterial, anti-diabetic (α-amylase (77%), α-glucosidase (78%) at 30 μg/ml, antioxidant (85–90%), anti-inflammatory (45–60%) properties and dye degrading properties. The antibacterial efficacy of silver nanoparticles was assessed against pathogenic species of <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> reporting inhibitory zones of 13 ± 0.3 and 21 ± 0.2 mm, respectively comparable to the standard Gentamycin (22 ± 0.4 mm). The synthesized AgNPs demonstrated excellent dye degradation kinetics in non-photocatalytic dyes methyl orange (96 %; 30 min) and safranin O (95 %; 40 min) along with experimental reusability found to be satisfactorily upto 7 cycles.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100600"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145362230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilizing deep learning algorithms for the early identification and categorization of skin cancer","authors":"Junaid Iqbal ,&nbsp;Mohammad Faisal ,&nbsp;Subhan Ullah ,&nbsp;Zareen A. Khan ,&nbsp;Zeeshan Ali ,&nbsp;Noor zaman Bawari","doi":"10.1016/j.jgeb.2025.100576","DOIUrl":"10.1016/j.jgeb.2025.100576","url":null,"abstract":"<div><div>Skin cancer is one of the utmost global challenges for today human being. Diverse forms of skin cancer found in humans, but current research work focuses on malignancy. The malignant can be treated easily if detected in the early stage. To achieve this goal, image processing and deep learning techniques were performed for the distinguished melanoma in early stages. Utilizing three models such as EfficientNet-B0, VGG16, and Inception-V3, the process involved initial preprocessing of image followed by training these models for 30 epochs on the PH2 and ISIC datasets. All three models demonstrated strong performance on both dataset; however, EfficientNet-B0 outperformed the other two models with an accuracy of 92 %, while Inception-V3 achieved 87 % accuracy and VGG-16 achieved 85 % accuracy. It has been established from the results that we aspire to enhance our suggested idea by incorporating it into a mobile platform in forthcoming endeavors. This modification will allow users to access and employ the model’s capabilities on the go, enhancing its reach and impact. The mobile platform will have an easy-to-use interface, allowing users to provide data and get reliable outcomes. This integration will not only increase the accessibility of the model, but also improve its value, making it easier for individuals to adopt it into their daily lives. By doing so, we hope to boost the model’s adoption and utilization, resulting in more accurate predictions and better decision-making.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100576"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}