Journal of Genetic Engineering and Biotechnology最新文献

{"title":"Loss-of-function variant of SLC30A8 rs13266634 (C > T) protects against type 2 diabetes by stabilizing ZnT8: Insights from epidemiological and computational analyses","authors":"Nilima Barman ,&nbsp;M Atiqul Haque ,&nbsp;Md Ridwan ,&nbsp;Debabrata Ghosh ,&nbsp;Abul B.M.M.K. Islam","doi":"10.1016/j.jgeb.2025.100565","DOIUrl":"10.1016/j.jgeb.2025.100565","url":null,"abstract":"<div><div>The loss-of-function variant rs13266634 (C &gt; T) in <em>SLC30A8</em> has been linked to type-2-diabetes (T2D) susceptibility with conflicting findings. We investigated its role among T2D patients of Bangladesh. The rs13266634 (C &gt; T) variant results in an arginine-to-tryptophan (p.R325W) substitution of <em>SLC30A8</em>-encoded zinc transporter protein, ZnT8. As zinc is crucial for insulin-crystallization for storage, we analyzed its wild-type (WZnT8) and variant (MZnT8) protein conformations via molecular-dynamics (MD) simulations. For the epidemiological analysis, a case-control study was conducted with 90 T2D patients from a diabetic-patient-cohort and 90 gender-matched healthy-controls. Genomic DNA was isolated from blood samples and genotyped by Sanger sequencing. MD simulations, spanning over 100 ns, were executed using GROMACS v2024.4. The homozygous wild CC-genotype was significantly more frequent in T2D patients (66.7 %) than in control (50.0 %) and was increasing T2D risk, with adjusted odds-ratio (OR_adj) of 2.25 under dominant model. Conversely, the polymorphic TT-genotype showed a protective effect, with an OR_adj of 0.196 (95 % CI:0.045–0.850, p = 0.029) in an additive model. MD simulations revealed that MZnT8 variant exhibited higher structural stability, lower flexibility and a more compact conformation than WZnT8. In conclusion, the polymorphic TT-genotype of <em>SLC30A8</em> plays a protective role in T2D susceptibility. Its protein variant, MZnT8, adopts a stable, compact conformation with lower flexibility, which may decrease zinc transport in insulin secreting granules and impair insulin crystallization. This structural adaptation could enhance glucose-stimulated insulin secretion and ultimately contribute to T2D protection.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100565"},"PeriodicalIF":2.8,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotyping analysis of rice cv. Nipponbare overexpressing OsMADS56 gene in drought tolerance, plant height, and yield components","authors":"Dini Nurdiani,&nbsp;Dwi Widyajayantie,&nbsp;Enung Sri Mulyaningsih,&nbsp;Amy Estiati,&nbsp;Satya Nugroho","doi":"10.1016/j.jgeb.2025.100570","DOIUrl":"10.1016/j.jgeb.2025.100570","url":null,"abstract":"<div><h3>Background</h3><div>The MADS-box protein family is a transcription factor (TF) family whose members harbour a MADS-box domain located in their N-terminal. This domain consists of 60 amino acids and functions in DNA binding. In plants, these family genes broadly regulate plant growth, development and responses to various environmental stresses. In this study, an <em>OsMADS56</em> (702 bp) was isolated from rice (<em>Oryza sativa</em> cv. Nipponbare) to investigate its role in response to drought stress and other agronomic phenotypes at both the vegetative and generative stages.</div></div><div><h3>Results</h3><div>Rice mutants harbouring overexpressed <em>OsMADS56</em> gene driven by the constitutive 35S promoter were generated. Quantitative real-time (qRT)-PCR confirmed the overexpression of <em>OsMADS56</em> at T1 (M76.7) generation. Observation of the T2 <em>OsMADS56</em>-overexpressed rice line (OX7) showed improved tolerance to drought stress. It also showed different agronomic phenotypes compared to wild type (WT), such as shorter plant height (PH) and improved productivity as indicated by earlier heading date (HD), a higher number of panicles (NP), a higher number of grains (NG) and higher grain yield (GY) than WT.</div></div><div><h3>Conclusion</h3><div>The findings suggest that <em>OsMADS56</em> was implicated in the rice response to PEG-induced drought stress, rice plant height, and yield components. The <em>OsMADS56</em> gene and the characterized line can be utilized in further studies on rice adaptation against abiotic stress and its productivity.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100570"},"PeriodicalIF":2.8,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic characterization and proteomic profiling of venoms from the medically important Androctonus species","authors":"Alhussin M.A. Megaly ,&nbsp;Masahiro Miyashita ,&nbsp;Abdulaziz R. Alqahtani ,&nbsp;Mohammed Abdel-Wahab","doi":"10.1016/j.jgeb.2025.100566","DOIUrl":"10.1016/j.jgeb.2025.100566","url":null,"abstract":"<div><div>Egyptian scorpions of the <em>Androctonus</em> genus (family Buthidae) produce life-threatening stings owing to their neurotoxic venom. However, the composition and enzymatic activities of their venoms remain poorly understood: We used electrophoresis to analyze the protein components of venoms collected from three <em>Androctonus</em> species: <em>Androctonus amoreuxi</em>, <em>Androctonus australis</em>, and <em>Androctonus bicolor</em>. Mass spectrometric analysis was performed to characterize the peptides present in these venoms. The phospholipase A₂ (PLA₂), hyaluronidase, and protease activities of the venoms were examined to gauge their potential contribution to venom toxicity. Finally, the antibacterial and hemolytic activities of the venoms were evaluated. The electrophoretic profiles of the three venoms showed features specific to each species, with distinct protein bands observed at 75, 74, 67, 48, 46, 40, and 28 kDa, along with a notable band above the 15-kDa mark. Liquid chromatography/mass spectrometry analyses were used to detect the presence of 369, 324, and 351 components in with molecular masses in the range of 500–10,000 Da in the venoms of <em>A. amoreuxi</em>, <em>A. australis</em>, and <em>A. bicolor</em>, respectively. Disulfide-rich peptides (three disulfide bridges) were abundant, but peptides without disulfide bonds were also detected in all venom samples. All three venoms exhibited hyaluronidase activities, whereas protease and PLA₂ activities were either weak (at 1 µg and 10 µg) or undetectable, even at higher concentrations (up to 20 µg). All assays were performed using venoms standardized by dry weight to ensure consistent protein quantities. Crude venoms of <em>A. amoreuxi</em> and <em>A. australis</em> showed antibacterial activity against <em>E. coli</em> and <em>B. subtilis</em> (5–10 μg), whereas <em>A. bicolor</em> required 10 μg. Hydrophobic fractions (40–55 min) of <em>A. australis</em> alone retained this activity. This work furthers our knowledge of the enzymatic and peptide composition of <em>Androctonus</em> venoms, unveiling their potential in drug delivery enhancement and other biomedical applications. These findings will inform the development of better strategies for the treatment and prevention of scorpion envenomation.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100566"},"PeriodicalIF":2.8,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding the cannabis tissue culture puzzle: Machine learning analysis of cannabis in vitro morpho-physiological disorders expands the potential for precision micropropagation","authors":"Marco Pepe,&nbsp;Mohsen Hesami,&nbsp;Kristian Adamek,&nbsp;Andrew Maxwell Phineas Jones","doi":"10.1016/j.jgeb.2025.100569","DOIUrl":"10.1016/j.jgeb.2025.100569","url":null,"abstract":"<div><div><em>Cannabis sativa</em> L. (cannabis) has recently re-emerged as an economically important crop, fueling research focused on enhancing production practices to meet market demands. The developing cannabis industry can be improved by overcoming certain production hurdles using micropropagation to maintain and multiply pathogen-free plants in confined spaces at high volumes. However, developing efficient micropropagation systems for cannabis have been hampered by the prevalence of various morpho-physiological disorders, resulting in low multiplication rates, culture decline, and overall low efficiency rates. While progress in cannabis micropropagation has been made, nutrient imbalances and various disorders are still common. Successful micropropagation is species specific and dependent on a variety of interconnected factors related to abiotic conditions and nutrient availability, which represent challenges in the refinement and execution of effective methods. Micropropagation media represent the exclusive sources of macro- and micro-nutrients for cultured plant tissues, inadequacies of which can result in the emergence of morpho-physiological symptoms. This work represents the first in-depth analysis of multiple morpho-physiological disorders in micropropagated cannabis arising from media nutrient content. Additionally, we present machine learning as an effective tool for assessing nutrient-associated symptoms in cultured cannabis and identifying which components are responsible. Results will help with troubleshooting cannabis micropropagation systems to prevent or correct undesirable outcomes, while introducing new methods to assess <em>in vitro</em> cannabis disorders.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100569"},"PeriodicalIF":2.8,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A metagenomic exploration of microbial distribution among the pond sediment, shrimp (Penaeus monodon) tissue and pond water: A special focus on nutrient cycling and shrimp viral pathogens","authors":"Avik Sarkar ,&nbsp;Rakesh Kr. Patra ,&nbsp;Mathavan Muthaiyan ,&nbsp;Ajoy Mallik","doi":"10.1016/j.jgeb.2025.100568","DOIUrl":"10.1016/j.jgeb.2025.100568","url":null,"abstract":"<div><div><em>Penaeus monodon</em>, a major shrimp species cultivated in Eastern Asia, has faced setbacks in aquaculture due to disease outbreaks. Metagenomic studies offer insights into the pond microbiome, revealing its critical roles in nutrient cycling, water quality, and disease control. This study investigated microbial communities in sediment, water, and shrimp samples from an aquaculture pond in Chaital, West Bengal, India. Shotgun metagenomic sequencing and bioinformatics analyses were used to assess taxonomic and functional profiles, focusing on nutrient cycling genes and viral populations. Sediments showed the highest microbial diversity, contributing to organic matter decomposition and ecological stability. Water microbes played roles in oxygenation and pathogen suppression, while shrimp tissues hosted microbes aiding digestion and immunity. Pathogenic viruses, especially Whispovirus (White Spot Syndrome Virus), dominated shrimp tissues. Functional gene analysis identified key pathways in carbon, nitrogen, and sulfur cycling. Integrating water quality data with microbiome profiles could help identify bioindicators and predict pathogen risks.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100568"},"PeriodicalIF":2.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-silico study on biomolecules derived from Cissus quadrangularis towards anti-inflammation","authors":"H. Nilofer Farjana ,&nbsp;G. Mohan Valiathan ,&nbsp;S. Mohanasatheesh","doi":"10.1016/j.jgeb.2025.100571","DOIUrl":"10.1016/j.jgeb.2025.100571","url":null,"abstract":"<div><h3>Objective</h3><div>CD 163 protein is a macrophage-specific protein and hemoglobin scavenger receptor. Elevated expression of CD 163 protein in macrophages is an important feature indicating that tissues are subjected to inflammation. It can be an effective diagnostic biomarker for inflammatory diseases like gingivitis and periodontitis. Cissus quadrangularis is used as a potent alternative medicine to control inflammation and heal fractured bones. It has high osteogenic potential by increasing osteoblast mineralization. Being a natural product with a wide range of benefits can lead to drug development for its high anti-inflammatory and anti-osteoporotic properties. Studies have shown that Cissus quadrangularis has significant anti-inflammatory potential and can be used as a potential target for therapeutic agents. The objective of the study is to explore the interaction of the phytonutrients derived from Cissus quadrangularis with CD 163 protein to establish the anti-inflammatory activity by in silico approach.</div></div><div><h3>Materials and methods</h3><div>The biomolecules derived from the plant Cissus quadrangularis namely β-Sitosterol, luteolin, piceatannol, quadrangualrin, resveratrol, and tannins were subjected to molecular docking using Autodock of 4.0 PyRx software.</div></div><div><h3>Results</h3><div>The study results showed drug-likeness and binding affinity between biologically active Cissus quadrangularis molecules against inflammation associated with CD 163 protein.</div></div><div><h3>Conclusion</h3><div>It can be concluded that the biomolecules derived from Cissus quadrangularis exhibited anti-inflammatory potential and thus it can be further explored by invitro analysis in developing a new drug.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100571"},"PeriodicalIF":2.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic differentiation and conservation insights into Salicornia iranica subsp. sinus-persica from Musa Bay using SCoT markers and DNA barcodes","authors":"Fatemeh Nasernakhaei ,&nbsp;Mahyar Zahraei","doi":"10.1016/j.jgeb.2025.100563","DOIUrl":"10.1016/j.jgeb.2025.100563","url":null,"abstract":"<div><div>Understanding genetic diversity is essential for the conservation and management of halophyte species, especially in ecologically sensitive and industrially impacted regions. In this study, 52 accessions of <em>Salicornia iranica</em> subsp. <em>sinus-persica</em> were collected from diverse microhabitats across Musa Bay, including coastal zones, islands, and petrochemical sites. Following comprehensive morphological assessments showing high phenotypic similarity, and to ensure broad ecological coverage, 16 accessions representing all major microhabitats were selected for molecular analysis. SCoT markers and DNA barcoding (ITS and <em>trnH-psbA</em> regions) were employed to investigate their genetic structure. The SCoT markers revealed considerable polymorphism and partial genetic differentiation, especially between accessions from petrochemical and natural saline habitats. ITS sequencing identified five haplotypes without forming a monophyletic clade, indicating shallow divergence and possible ancestral polymorphism. In contrast, no variation was detected in the plastid region <em>trnH-psbA</em>. These results represent the first global report of ITS and <em>trnH-psbA</em> haplotypes for this subspecies. While the findings are preliminary due to limited sample size and marker coverage, they provide a critical baseline for future studies employing high-resolution genomic tools. Our findings underscore the influence of habitat fragmentation and anthropogenic pressures on genetic patterns and emphasize the importance of conserving genetically distinct accessions in ecologically vulnerable habitats to support the long-term survival and sustainable utilization of this coastal halophyte.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100563"},"PeriodicalIF":2.8,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical screening and inhibitory effects of Catha edulis Forsk extracts on oxidation, growth, biofilm and quorum sensing of selected pathogens","authors":"Yonatan Nesru ,&nbsp;Muktar Ahmed ,&nbsp;Adam Mengistu ,&nbsp;Mohammed Naimuddin","doi":"10.1016/j.jgeb.2025.100560","DOIUrl":"10.1016/j.jgeb.2025.100560","url":null,"abstract":"<div><div>The treatment of infectious diseases is increasingly challenging due to the emergence and re-emergence of antibiotic-resistant bacteria. Plant-derived natural products are being explored as potential solutions to this global threat. This study investigated the phytochemical composition of <em>Catha edulis</em> and biological properties of its extracts, focusing on their potential as antioxidants and antimicrobials. Gas chromatography/mass spectrometry (GC/MS) of the methanol extract (MeOHE) fractions revealed 11 compounds in dichloromethane fraction (DCMF) and 30 compounds in ethyl acetate fraction (EAF), many of which possess bioactive functions as antioxidants and antimicrobials. Phytol was detected at 60.32 %, not reported before in <em>C. edulis</em>. The antioxidation potential of extracts was assayed by radical scavenging activity (RSA), and the IC₅₀ values ranged from 68.2 to 89.3 µg/mL, respectively indicating good antioxidant potency. In the disc diffusion assay against representative pathogenic bacteria, the extracts exhibited an inhibition order of EAF &gt; DCMF &gt; MeOHE in the 31–75 % range. The minimum inhibitory concentration (MIC) for MeOHE and DCMF were in the 0.5 − &gt;1 mg/mL range, while the minimum bactericidal concentration (MBC) were from 2 to &gt; 2 mg/mL. EAF showed a MIC and MBC in the 0.5–––1 mg/mL and 1–––2 mg/mL range, suggesting better inhibition potential. The tolerance test values showed bactericidal activity against all the tested bacteria. The minimum biofilm inhibitory concentration (MBIC) values of DCMF were 250 to &gt; 500 µg/mL, while EAF ranged from 125 to 500 µg/mL, indicating effective inhibition of biofilm formation. The anti-quorum sensing (anti-QS) activity of DCMF and EAF on <em>P. aeruginosa</em> showed 55.8 % and 51.4 % inhibition of pyocyanin production at 500 µg/mL. These results suggest that DCMF and EAF of <em>C. edulis</em> are potent antioxidants and antimicrobials, potentially reducing the load of pathogens. To our best knowledge, this is the first report of antibiofilm and anti-QS activities of <em>C. edulis</em> extracts on pathogenic bacteria included in the Pathogen Priority List.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100560"},"PeriodicalIF":2.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the therapeutic potential of microRNAs: targeted gene regulation strategies for enhanced cancer therapy","authors":"Shashwat Pandey ,&nbsp;Preeti Yadav","doi":"10.1016/j.jgeb.2025.100556","DOIUrl":"10.1016/j.jgeb.2025.100556","url":null,"abstract":"<div><div>Cancer remains one of the most significant causes of death worldwide, with conventional therapies often hindered by drug resistance, off-target effects, and systemic toxicity. MicroRNAs (miRNAs), small non-coding RNA molecules, have emerged as critical regulators of gene expression, influencing various cellular processes such as proliferation, apoptosis, and metastasis. Their dual role as tumor suppressors and oncogenes makes them promising therapeutic targets for cancer treatment. This review explores the therapeutic potential of miRNAs in modulating key cancer pathways and overcoming drug resistance. It delves into strategies like miRNA mimics and antagomiRs to restore normal gene expression, alongside advanced delivery systems, including nanoparticles and exosomes, to ensure targeted, stable, and effective delivery. Furthermore, the challenges associated with miRNA therapy, such as degradation, off-target effects, and immune responses, are discussed. The integration of miRNA-based approaches with existing therapies holds promise for enhancing treatment efficacy and paving the way for personalized cancer management.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100556"},"PeriodicalIF":2.8,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144907311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of Canarium odontophyllum fruit phytoconstituents as potential candidates against epilepsy using in silico studies","authors":"Lim Joe Siang ,&nbsp;Kamini Vijeepallam ,&nbsp;Arunachalam Muthuraman ,&nbsp;Parasuraman Pavadai ,&nbsp;Thiruventhan Karunakaran ,&nbsp;Veerasamy Ravichandran","doi":"10.1016/j.jgeb.2025.100561","DOIUrl":"10.1016/j.jgeb.2025.100561","url":null,"abstract":"<div><div>Epilepsy is the third most common neurological illness worldwide, affecting over 65 million people. Current antiepileptic drugs (AEDs) are ineffective for about 30 % of epileptic patients. AED use is extremely expensive and associated with adverse effects such as ataxia, nystagmus, and central nervous system depression. Serious quality-of-life changes are another aspect of managing epileptic conditions. A variety of therapeutic plant components, including flavonoids, phenolics, terpenoids, and alkaloids, exhibit a wide range of biological actions, including antibacterial, antioxidant, antiulcer, antineoplastic, anti-inflammatory, antiepileptic, and immunostimulant properties. Because many of them are being researched in modern research to develop an active therapeutic candidate, we resorted to medicinal plants to investigate powerful antiepileptic components. So, in this in silico study, we aimed to identify potential antiepileptic agents from <em>Canarium odontophyllum</em> fruits using molecular docking, molecular dynamics, and ADMET prediction approach. In the present study, the virtual screening was performed on a set of 63 phytocompounds, identified from the hydroalcoholic (70 %) extract of <em>C. odontophyllum</em> fruit using LC-MS (LC-MS-QTOF) analysis, by using molecular docking simulation to examine their binding ability with seven proteins that are responsible for epilepsy. Further, the selected compounds with good binding affinity were subjected to ADMET screening to predict the pharmacokinetic properties, and molecular dynamics testing was done to confirm the outcomes. Our results revealed that <em>C. odontophyllum</em> phytoconstituents showed the strongest binding affinities against all tested enzymes. Moreover, kanzonol B could prominently interact with all tested proteins with moderate to good binding affinity (−6.70 to −10.65 kcal/mol). All top phytoconstituents obeyed Lipinski’s RO5 and drug-likeness properties. These computational studies suggested that the phytoconstituents of <em>C. odontophyllum</em> can serve as potent inhibitors of enzymes responsible for epilepsy. Thus, we recommend further experimental investigations to validate the promising results of the present in silico study.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100561"},"PeriodicalIF":2.8,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144904237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}