Journal of Genetic Engineering and Biotechnology最新文献

{"title":"Deciphering genetic relationships within and among banana (Musa spp.) genome groups using ISSR and SRAP markers","authors":"Roshida Soraisham ,&nbsp;Punshi Tongbram ,&nbsp;Surendrakumar Singh Thingnam ,&nbsp;Boris Aheibam ,&nbsp;John Zothanzama ,&nbsp;Dinamani Singh Lourembam ,&nbsp;Robert Thangjam","doi":"10.1016/j.jgeb.2025.100540","DOIUrl":"10.1016/j.jgeb.2025.100540","url":null,"abstract":"<div><div>Characterization and identification of banana into their correct genome groups has been a problematic issue based on morphological and unappropriated molecular markers. In the present study, the genetic relationship between and among 28 banana (<em>Musa</em> spp.) accessions representing 5 genome groups (AAA, BB, AAB, ABB and AB) were evaluated using sequence-related amplified polymorphism (SRAP) and inter simple sequence repeat (ISSR) markers. Although similar genetic relationship parameters were observed with the two markers, the higher values were generated with SRAP profiles as indicated with mean gene diversity value of 0.69 with SRAP and 0.64 with ISSR, polymorphic information content (PIC) of 0.64 with SRAP and 0.59 with ISSR, total genetic diversity (<em>Ht</em>) of 0.39 with SRAP and 0.34 with ISSR, gene diversity within population (<em>Hs</em>) of 0.19 with SRAP and 0.12 with ISSR, coefficient of gene differentiation (<em>Gst</em>) value of 0.51 with SRAP and 0.62 with ISSR and gene flow (<em>Nm</em>)value of 0.51 with SRAP and 0.31 with ISSR. The resulting dendrogram and the population structure also supports and showed the concurrence with the genetic relationship parameters based on the genome groups of the accessions studied. The present finding, also indicates the higher efficacy of SRAP markers over the ISSR markers in the discrimination of banana accessions based on their genome groups by grouping the banana accessions belonging to the different genome groups and ploidy levels. The present finding could be utilized for effective discrimination of banana accessions belonging to different genome groups.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100540"},"PeriodicalIF":3.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A causal relationship between circulating immune cells, plasma metabolites, and pulmonary diseases: a mediated Mendelian randomization study","authors":"Chenyi Zhao ,&nbsp;Xuefei Song ,&nbsp;Dan Yang ,&nbsp;Chenbing Lv ,&nbsp;Yuhan Li ,&nbsp;Abualgasim Elgaili Abdalla ,&nbsp;Tingyu Shi ,&nbsp;Guirong Wang ,&nbsp;Longxiang Xie","doi":"10.1016/j.jgeb.2025.100537","DOIUrl":"10.1016/j.jgeb.2025.100537","url":null,"abstract":"<div><h3>Background</h3><div>Immune cells and plasma metabolites may play important roles in the development of pulmonary diseases, but the relationship between different immune cells, plasma metabolites and various pulmonary diseases is still unclear. In this study, we aim to employ Mendelian randomization (MR) to investigate the causality between immune cells, pulmonary diseases and plasma metabolites.</div></div><div><h3>Methods</h3><div>We analyzed immune cells and seven pulmonary diseases, including interstitial lung disease (ILD), idiopathic pulmonary fibrosis (IPF), lung cancer, pneumonia, chronic obstructive pulmonary disease (COPD), sleep apnea syndrome (SAS), and tuberculosis (TB), using genome-wide association analysis (GWAS) data for immune cells as an exposure factor and seven lung diseases as outcomes. Plasma metabolites GWAS data served as mediators. We applied MR analysis to explore the relationship between immune cells and pulmonary diseases, followed by two-step mediation analysis to identify potential metabolites that may mediate this association.</div></div><div><h3>Results</h3><div>As shown in the results, immune cells contribute to disease progression by reducing the protective effect of metabolites on disease or enhancing the promoting effect of metabolites on disease. These include CD4/CD8br and lung cancer, CD62L-CD86+ myeloid DC AC and Pneumonia, and Naive DN (CD4-CD8-) %T cell and COPD. On the other hand, immune cells suppress disease by increasing the inhibitory effect of metabolites on disease or decreasing the promoting effect of metabolites on disease, These include CD39 on CD39+ CD8br and ILD, CD28+ CD45RA+ CD8br AC and TB, Activated &amp; secreting Treg AC and SAS, HLA DR on DC and IPF.</div></div><div><h3>Conclusions</h3><div>We clarify the importance of the potential mechanisms pertaining to immune cells, metabolites, and pulmonary diseases, highlighting the complex interactions among these factors. This understanding may assist in the diagnosis and treatment of patients with pulmonary diseases.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100537"},"PeriodicalIF":3.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling genetic diversity in pointed gourd (Trichosanthes dioica) genotypes from India’s Eastern plateau and hill region: Insights from morphological and molecular markers","authors":"Ankit Kumar Sinha ,&nbsp;P. Bhavana ,&nbsp;A.K. Singh ,&nbsp;J.K. Ranjan ,&nbsp;H. Choudhary ,&nbsp;G.P. Mishra ,&nbsp;K. Thamilarsi ,&nbsp;Paresh Chaukhande ,&nbsp;Reshma Shinde ,&nbsp;Prakash Kumar ,&nbsp;Jitendra Rajak ,&nbsp;Sajiya Ekbal","doi":"10.1016/j.jgeb.2025.100542","DOIUrl":"10.1016/j.jgeb.2025.100542","url":null,"abstract":"<div><div>Pointed gourd (<em>Trichosanthes dioica</em> Roxb.), a nutritionally rich, dioecious cucurbit crop native to India, holds immense potential for enhancing food security, yet its genetic diversity remains underexplored, limiting breeding efforts for improved yield and quality. This study addresses this gap by evaluating the genetic diversity of 46 pointed gourd genotypes using morphological traits and ISSR markers. Principal component analysis (PCA) revealed three principal components explaining 72.53% of the total morphological variation, with fruit weight (0.47), pulp weight (0.46), and fruit volume (0.44) as primary contributors to PC1, and total fruit yield (0.53) and number of fruits per plant (0.49) dominating PC2. The PCA biplot identified five distinct genotype groups, highlighting significant diversity. Molecular analysis with sixteen polymorphic ISSR markers generated 96 bands, of which 76 were polymorphic (78.91%), with Polymorphism Information Content (PIC) ranging from 0.35 to 0.47. Cluster analysis grouped genotypes into seven morphological and two primary molecular clusters, the latter further divided into six sub-clusters. Swarna Rekha, Swarna Suruchi, Swarna Alaukik, HAP 24, HAP 78, and HAP 113 were identified as highly diverse. These findings demonstrate that integrating morphological and molecular markers effectively uncovers genetic variability, providing a robust foundation for breeding programs. Future work will focus on leveraging these diverse genotypes for hybridization to develop high-yielding, nutrient-rich cultivars and exploring additional molecular markers to further refine genetic diversity assessments.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100542"},"PeriodicalIF":3.5,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro and bioinformatic studies of bioactive compounds from Oceanimonas sp. JM-AZM31 and Lysinibacillus fusiformis JM-AZM37 of sponge-associated marine bacteria from a mangrove habitat in Southeast Sulawesi","authors":"Jendri Mamangkey ,&nbsp;Corrina Lailatul Fadjri ,&nbsp;Sunarto ,&nbsp;Apon Zaenal Mustopa ,&nbsp;Dwi Suryanto ,&nbsp;Nabila Swarna Puspa Hermana ,&nbsp;Nur Arfa Yanti ,&nbsp;Kusmiati Kusmiati ,&nbsp;Herman Irawan ,&nbsp;Adrian Hartanto ,&nbsp;La Ode Adi Parman Rudia ,&nbsp;Rizna Akmaliyah ,&nbsp;Lucas William Mendes ,&nbsp;Ferdin","doi":"10.1016/j.jgeb.2025.100538","DOIUrl":"10.1016/j.jgeb.2025.100538","url":null,"abstract":"<div><div>The ongoing quest for novel therapeutic agents has directed attention toward bioactive compounds derived from sponge-associated bacteria. This study focuses on sponge symbiont bacteria from the mangrove ecosystems in Tanjung Tiram, Southeast Sulawesi, which have not yet been reported for their potential antibacteria, anti-inflammatory, antioxidant, and anti-diabetic activities. The screening of marine bacterial isolates was performed using a series of assays: disc diffusion method to assess antibacterial activity, protein denaturation to assess anti-inflammatory properties, DPPH free radical scavenging to evaluate antioxidant capacity, and α-Glucosidase inhibition for anti-diabetic activity, followed by <em>in silico</em> validation. Two promising strains, identified through molecular techniques were designated as <em>Oceanimonas</em> sp. JM-AZM31 and <em>Lysinibacillus fusiformis</em> JM-AZM37. Initial bioactivity screening revealed significant potential: The bacterial isolates JM-AZM31 and JM-AZM37 demonstrated broad-spectrum antibacterial activity against both Gram-positive pathogens (<em>Bacillus cereus, Staphylococcus aureus, Staphylococcus epidermidis</em>) and Gram-negative pathogens (<em>Escherichia coli, Salmonella typhimurium</em>). JM-AZM31 exhibited an anti-inflammatory inhibition rate of 76.9 ± 2.90 %, antioxidant activity of 80.3 ± 1.02 %, and anti-diabetic activity of 84.9 ± 0.49 %. Similarly, JM-AZM37 showed anti-inflammatory activity of 71.6 ± 1.85 %, antioxidant activity of 76.9 ± 0.03 %, and anti-diabetic activity of 83.2 ± 1.27 %. Further analysis using GC–MS identified five significant compounds, which were examined through in silico molecular docking. Results indicated that n-hexadecanoic acid, DL-proline, 5-oxo-, and <em>cis</em>-vaccenic acid showed high binding affinities to specific therapeutic targets, suggesting strong potential as biotherapeutic agents. This current inquiry concentrates on the therapeutic potential of marine bacteria from mangrove ecosystems as a source of bioactive compounds, positioning <em>Oceanimonas</em> sp. JM-AZM31 and <em>L. fusiformis</em> JM-AZM37 as promising candidates for developing new biotherapeutic treatments.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100538"},"PeriodicalIF":3.5,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"cfDNA Key genomic markers in HCV-Induced hepatocellular carcinoma in Egyptian patients","authors":"Mohamed Khalifa ,&nbsp;Ahmed A. Hmed ,&nbsp;Khaled S. Elfeky ,&nbsp;Sayed Bakry ,&nbsp;Manal El Hamshary ,&nbsp;Ahmed R. Sofy","doi":"10.1016/j.jgeb.2025.100533","DOIUrl":"10.1016/j.jgeb.2025.100533","url":null,"abstract":"<div><h3>Background</h3><div>Malignant liver disease is among the highest in the world, with hepatocellular carcinoma (HCC) accounting for up to 90 % of all cases. In Egypt, HCC poses a significant public-health concern, representing 47.17 % of cancer cases. The high incidence of hepatitis C virus (HCV) in the Egypt was a major predisposing factor for HCC.</div></div><div><h3>Material</h3><div>This study included 63 Egyptian HCC patients, 55 % of whom had a history of HCV infection.</div></div><div><h3>Methods</h3><div>Using a paired sampling strategy, approximately 2800 COSMIC mutations from 50 oncogenes and tumor-suppressor genes were NGS sequenced.</div></div><div><h3>Results</h3><div>Total of 381 somatic mutations were identified, 91 mutations detected in the HCC group and 291 in the HCV-related HCC group. The top 10 mutated genes in the non-HCV group were TP53, ATM, EGFR, CDH1, FGFR1, MET, SMAD4, ERBB2, FLT3, and FBXW7, while in the HCV-related HCC group, genes were KIT, ATM, TP53, APC, FBXW7, KDR, RB1, SMAD4, EGFR, and PIK3CA.</div></div><div><h3>Conclusion</h3><div>The present study represents the first comprehensive somatic mutation profile in HCC Egyptian patients. This finding suggests that HCV viral infection played a direct and indirect role in increasing the somatic mutation burden in HCV-related HCC patients and opens new promises of targeted therapies for those patients.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100533"},"PeriodicalIF":3.5,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of plastics and pesticides by soil bacteria in Bangladesh: Insights into antibiotic resistance and potential therapeutic targets","authors":"Azrin Ahmed,&nbsp;Shahin Mahmud,&nbsp;Mst. Tania Khatun,&nbsp;Md. Firoz Ali,&nbsp;Mst Rony Akter,&nbsp;A.K.M. Mohiuddin","doi":"10.1016/j.jgeb.2025.100532","DOIUrl":"10.1016/j.jgeb.2025.100532","url":null,"abstract":"<div><div>Soil bacteria exhibit varying degrees of tolerance to different concentrations of pesticides and plastics, and some possess the ability to degrade them, which is crucial for bioremediation. However, the multidrug-resistant properties of these bacteria pose challenges for their potential applications. Hence, this study aims to separate and characterize plastics and pesticide-degrading bacteria from<!--> <!-->non-contaminated and contaminated sites in Bangladesh and evaluate their antibiotic-resistant patterns to identify safety issues and discover promising therapeutic targets for combating multidrug-resistant infections. In the current study, a total of 90 soil samples were collected from different agricultural and dumped sites of Bangladesh, and bacterial isolates were screened for pesticides and plastics-degrading capabilities. Antibiotic sensitivity patterns of the potential isolates were evaluated using 16 different antibiotics. Biochemical, molecular, and genomic analyses were conducted to characterize the bacteria and identify antimicrobial resistance (AMR) genes. Our study screened out 122 plastic and 60 pesticide-tolerant bacterial isolates. Among them, 3 pesticide and 3 plastic-degrading isolates were found to be more promising and identified as <em>Acinetobacter baumannii</em> with pesticide-degrading capabilities from non-contaminated sites, and <em>Klebsiella pneumoniae</em> with plastic-degrading capabilities from contaminated sites. Antibiotic sensitivity test exhibited that most of the isolates were resistance to commonly used antimicrobials. The genomics and proteomics analysis uncovered the efflux pump-related genes responsible for the resistant mechanism and highlighted the involvement of genes that respond to antibiotics and transmembrane transport activities. Phylogenetic analysis confirmed the conservation of 2 common resistance genes <em>adeF</em> and <em>gyrA</em>, across diverse multidrug-resistant pathogens. Therefore, targeting conserved genes <em>adeF</em> and <em>gyrA</em>, to disrupt resistance mechanisms and combat persistent and clinically significant multidrug-resistant pathogens could be a promising strategy for developing combination therapies in medical science.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100532"},"PeriodicalIF":3.5,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144631688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the role of Peanut (Arachis hypogaea L.) root architecture in enhancing adaptation to climate change for sustainable agriculture and resilient crop production: A review","authors":"Yohannes Gelaye ,&nbsp;Jihua Li ,&nbsp;Huaiyong Luo","doi":"10.1016/j.jgeb.2025.100535","DOIUrl":"10.1016/j.jgeb.2025.100535","url":null,"abstract":"<div><div>Peanut (<em>Arachis hypogaea</em> L.) cultivation is increasingly vulnerable to climate change, with drought and heat stress emerging as major constraints to productivity and food security. This review explores the critical role of root architecture in enhancing peanut adaptation to environmental stressors, and evaluates current strategies and future directions for improving root traits through genetic, physiological, and agronomic approaches. Efficient root systems, characterized by deeper rooting and optimized xylem design, significantly improve water and nutrient acquisition under drought conditions. Key regulators such as abscisic acid (ABA), strigolactones, and specific root-related genes modulate root development and stress responses. Root exudates further enhance soil root interactions, while the peanut root microbiome contributes to nutrient cycling and resilience. Biotechnological tools, including quantitative trait loci (QTL) mapping and CRISPR/Cas-based genome editing, are being harnessed to manipulate root traits at the molecular level. Agronomic practices like mulching and cover cropping synergize with genetic improvements by enhancing soil structure and moisture retention. Strengthening peanut root architecture through the integration of modern breeding, biotechnological advances, and sustainable soil management offers a promising path toward climate-resilient peanut production. Future research should prioritize the convergence of these approaches, alongside microbiome exploration, to secure yield stability and food security in a changing climate.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100535"},"PeriodicalIF":3.5,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144596503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolution of superbugs in space: a genomic perspective on pathogens in the International Space Station environment","authors":"Sara Pearl ,&nbsp;Hithesh Kumar ,&nbsp;Santhiya Vijayakumar ,&nbsp;Soumya Basu ,&nbsp;Sudha Ramaiah ,&nbsp;Anand Anbarasu","doi":"10.1016/j.jgeb.2025.100536","DOIUrl":"10.1016/j.jgeb.2025.100536","url":null,"abstract":"<div><div>Microgravity, pressure, and temperature variations in the International Space Station (ISS) create conditions leading to the emergence of superbugs. Due to technical issues in spacecraft, astronauts are forced to stay in ISS for extended periods; prolonged stay and exposure in stressful ISS environment weakens their immune systems, increasing susceptibility to infections. The presence of hypervirulent and antibiotic-resistant pathogens in space station is a worrisome feature as these might cause serious life-threatening infections in astronauts staying in high stress environments with weakened immune systems. In the present study, we compared antimicrobial resistance genes (ARGs) and virulence factors (VFs) in bacterial genomes from ISS with Earth counterparts. ISS genomes exhibited elevated counts of defense-related genes, particularly in <em>E. ludwigii</em> and <em>E. cancerogenus</em>. Among genes uniquely found in ISS genomes, CRISPR-Cas system components were notably prevalent. Though Earth genomes harbored higher number of ARGs overall, several species from ISS possessed modestly higher ARG counts. VFs profiling showed a slightly lower count in ISS genomes, but <em>P. conspicua</em>, <em>E. ludwigii</em>, and <em>K. pneumoniae</em> from ISS carried exclusive VFs linked to metal ion uptake and secretion systems, suggesting environment-driven functional adaptations. The adaptation of pathogenic bacteria in ISS is alarming and therefore periodic monitoring of bacterial genomic surveillance is important. Our findings shed light on genomic profiles in bacterial strains from both ISS and Earth, enhancing our understanding of the bacterial pathogens’ potential impact on drug resistance and pathogenicity in space-missions and the possible threat of spread from ISS.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100536"},"PeriodicalIF":3.5,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting cholinergic dysfunction in Alzheimer’s disease: Development of next-generation galantamine derivatives","authors":"Zahra Keshavarz ,&nbsp;Asadollah Hassankhani ,&nbsp;Mehdi Yoosefian","doi":"10.1016/j.jgeb.2025.100530","DOIUrl":"10.1016/j.jgeb.2025.100530","url":null,"abstract":"<div><div>Alzheimer’s disease (AD) presents a significant and unmet medical need. Acetylcholinesterase (AChE) enzymes are key contributors to AD pathogenesis, instigating a cholinergic deficit and subsequent neurodegenerative progression, leading to neuronal loss and cognitive decline. This study leverages in silico methods to elucidate the structure–activity relationships (SAR) governing binding interactions and affinity between human AChE (hAChE, PDB ID: 4EY6) and potential novel ligands. Galantamine, a cornerstone cholinergic therapy for AD, serves as the template for the design of structurally diverse galantamine analogs. Employing rigorous molecular docking simulations and molecular dynamics simulations, these analogs exhibit promising potential for modulating the cholinergic system and mitigating cognitive dysfunction associated with AD. Our findings demonstrate the efficacy of computational drug design in developing superior galantamine analogs with enhanced target selectivity and potentially improved therapeutic profiles for AD patients, aiming to achieve superior cognitive outcomes and improved quality of life.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100530"},"PeriodicalIF":3.5,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole exon screening of SLC2A4 gene and the association of rs5435 with type 2 diabetes in a Bangladeshi case-control study","authors":"Mohammad Mamunur Rashid ,&nbsp;Mohammad Sayem ,&nbsp;Maisha Adiba ,&nbsp;Abdullah Al Saba ,&nbsp;A.H.M. Nurun Nabi ,&nbsp;Tahirah Yasmin","doi":"10.1016/j.jgeb.2025.100534","DOIUrl":"10.1016/j.jgeb.2025.100534","url":null,"abstract":"<div><div>Type 2 diabetes (T2D) is a multifaceted disease influenced by both genetic and environmental factors, posing significant global health challenges. Polymorphisms in the <em>SLC2A4</em> gene encoding the glucose transporter 4 (GLUT4), have been linked to insulin resistance and T2D. This study, therefore, aims to identify and analyze genetic variants within the <em>SLC2A4</em> gene and determine their association with T2D risk through a Bangladeshi case-control study. A total of 239 individuals were enrolled in the study, including 127 T2D patients and 112 controls. All 11 exons of the SLC2A4 gene were sequenced in 20 individuals. Exons 2, 3, 4, and 5 of GLUT4 were then sequenced in an additional 99 participants. For the remaining 120 participants, a TaqMan probe-based RT-PCR was conducted to genotype rs5435. Four genetic variants were initially identified, including one synonymous variant (rs5435) in exon 4. There was no significant difference in the allele frequency of the wild-type allele T and variant allele C between the two groups (OR: 1.31, p = 0.13). Genotypic distribution revealed that individuals who were either heterozygous or homozygous for the variant allele are at an increased risk for developing T2D compared to those who are homozygous for the non-risk allele (p = 0.042), suggesting that the SNP could be considered to have a dominant effect on the likelihood of developing T2D. Additionally, <em>in silico</em> analysis revealed that the rs5435 variant introduces an additional loop in the mRNA secondary structure, which is energetically less stable and therefore may interfere with the protein’s biological function and play a role in the pathogenesis of T2D. However, further studies with larger cohorts are necessary to validate the impact of rs5435 on the risk of T2D in our population.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100534"},"PeriodicalIF":3.5,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}