Journal of Genetic Engineering and Biotechnology最新文献

{"title":"Morphological and genetic diversity assessment of cultivated Thymus satureioides (endemic species) of Morocco","authors":"Zoubida Belmahi ,&nbsp;Khadija Bakhy ,&nbsp;Fatima Gaboun ,&nbsp;Rabha Abdelwahd ,&nbsp;Karim Saghir ,&nbsp;Soumiya Ouedrhiri ,&nbsp;Hamid Khamar ,&nbsp;Hanaa Abdelmoumen ,&nbsp;Majid Mounir ,&nbsp;Ghizlane Diria","doi":"10.1016/j.jgeb.2025.100467","DOIUrl":"10.1016/j.jgeb.2025.100467","url":null,"abstract":"<div><div><em>Thymus satureioides</em> is an endemic and medicinal plant of Morocco, widely distributed in the arid and semiarid habitats. Communally used in traditional medicine. In the current study, twelve Inter-Simple Sequence Repeats (ISSR) primers combined with 11 agro-morphological traits were applied to evaluate 60 accessions of <em>T. satureioides</em> collected from 10 spontaneous sites covering most geographical area. These accessions were cultivated in two experimental stations Khémisset and Rabat. Variation coefficient of Phenotypic studied traits (CV) varied from 2.99 (inflorescence/stems) to 47.37 % (secondary branches/stem number). ANOVA showed very highly significant differences between accessions for of of all the studied traits (p &lt; 0.0001). The experimental station of Khémisset recorded the highest values compared to Rabat. PCA plot showed that 90.39 % were the most six variable morphological characters. At 80 %, Cluster analysis grouped the accessions into two major clusters based on their morphological resemblance. AMOVA revealed that the molecular variation within and between accessions was demonstrated to 82 % and 18 %, respectively. The number of bands is ranged from 12 for primer UBC825 to 28 for primer ISSR-4, those amplified 119 band and generating 739 amplicons. The UPGMA dendrogram, established through dissimilarity index, exhibited three groups. PCoA plot revealed three major groups of populations and consistent with genetic relationships derived from Cluster analysis. Tamssount region recorded high values of genetic diversity (He = 0.182), percentage polymorphic loci (PPL = 63.03 %) and Shannon information index (I = 0.283). These results highlighted a variability that will be useful for the breeding program aiming at improving the productivity, conservation and domestication of <em>Thymus satureioides</em>.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100467"},"PeriodicalIF":3.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity and population structure analysis of Indian blackberry (Syzygium cumini L.) using CAAT box‑derived polymorphism (CBDP) and start codon targeted polymorphism (SCoT) markers","authors":"Ajay Kumar ,&nbsp;Kanhaiya Singh ,&nbsp;Amit Kumar Singh ,&nbsp;Jai Prakash ,&nbsp;Amit Kumar Goswami ,&nbsp;Gyan Prakash Mishra ,&nbsp;Vishaw Bandhu Patel ,&nbsp;Suman Lata ,&nbsp;Anshuman Singh","doi":"10.1016/j.jgeb.2025.100468","DOIUrl":"10.1016/j.jgeb.2025.100468","url":null,"abstract":"<div><div>Indian blackberry (<em>Syzygium cumini</em> L.) also known as jamun is a very important underutilized fruit crop with notable medicinal and economic value. However, its genetic improvement has been constrained by limited knowledge of the genetic diversity within existing collections. Therefore, a comprehensive characterization of genetic diversity in this species, using molecular tools, is essential to support effective germplasm management and application in breeding programs. In this investigation, a total of 32 jamun genotypes consisting of 30 seedling-origin genotypes, one improved cultivar CISH J-37 and one wild genotype (<em>Syzygium fruitecosum</em>) were analysed using the two gene-targeted markers, CBDP and SCoT. In total, 29 primers (22 CBDP and 7 SCoT primers) detected genetic polymorphism across the genotypes. The CBDP markers amplified a higher polymorphism percentage, 94.85% across 291 bands, than the SCoT markers, 92.75% across 69 bands. The mean PIC values for CBDP and SCoT were 0.28 and 0.31, respectively. MI values were higher for CBDP (3.21) than for SCoT (2.88). Cluster analysis using UPGMA identified six clades, which grouped genotypes into seedling-origin, improved and wild categories. The PCoA based on molecular profiling data of CBDP, SCoT and both together explained 26.65%, 38.39% and 23.22% of the variation respectively. AMOVA results revealed that 85–90% of genetic variation existed within populations. Bayesian STRUCTURE analysis grouped genotypes into two major populations confirming genetic divergence between seedling-origin, improved and wild genotypes. This study is the first to integrate CBDP and SCoT markers for genetic diversity analysis of the Indian blackberry. The results highlight the utility of these markers in genetic variation assessment and would help design germplasm conservation and breeding strategies in this crop.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100468"},"PeriodicalIF":3.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management succinate release through SDHA by G protein-coupled receptor 91 signal, TRAP1, and SIRT3 regulation in lung cancer cells by NAR nanoparticles","authors":"Eman M. Ragab,&nbsp;Abeer A. Khamis,&nbsp;Tarek M. Mohamed,&nbsp;Doaa M. El Gamal","doi":"10.1016/j.jgeb.2025.100464","DOIUrl":"10.1016/j.jgeb.2025.100464","url":null,"abstract":"<div><h3>Background</h3><div>Cancer cells display oxidative metabolic dysregulation to fulfill their bioenergy requirements. Specifically, efforts were made to regulate the metabolite succinate and its negative effects as an inducer for neoplasm invasion and metastasis.</div></div><div><h3>Methods</h3><div>Binding affinity of naringenin (NAR) to mitochondria complex II (CΙΙ) subunits, sirtuin3 (SIRT3), tumor necrosis factor associate protein 1(TRAP1), and succinate receptor (SUCNR1) was studied by molecular docking. NAR nanoparticles (NARNPs) were synthesized and characterized by IR, X-ray, UV, drug release, zeta potential, TEM, and SEM. The IC₅₀ was evaluated in normal mice, normal fibroblast, and A549 cells by using the MTT technique. Moreover, the impact of NAR and NARNPs against 5-FLU on CΙΙ activity, SOD activity, and mitochondrial swelling was assessed. Apoptosis was also assessed using the flow cytometry method. While the expression of relevant genes such as SDHC, D, SIRT-3, TRAP1, SUCNR1, and ERK1/2 genes was determined by using RT-qPCR analysis. Western blot evaluated PI3K, NF-κB against β-actin.</div></div><div><h3>Results</h3><div>Theoretically, the binding affinity between NAR &amp; SDHC, D, SIRT-3, TRAP1, and SUCNR1 proteins was stronger. Cytotoxic effects of NAR and NARNPs were evaluated. Also, the activity of SDH C, and D was inhibited more than SDH A, and B activity in the A549 than normal cell lines (NARNPs &lt; NAR &lt; 5-FLU), This was accompanied by downregulation of SDH C, D, TRAP1, SUCNR1, and ERK1/2 genes expression, and upregulation of SIRT-3 gene expression. Additionally, NF-κB and PI3K protein expression declined. On the other hand, there was a significant increase in apoptotic effects with mitochondria enlargement (NARNPs &gt; NAR &gt; 5-FLU) in A549 compared with normal cells.</div></div><div><h3>In conclusion</h3><div>Controlling succinate by SDH parallel with SUCNR1 signal regulation by NARNPs will be a novel understanding mechanism and candidate for therapeutic target in lung cancer.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100464"},"PeriodicalIF":3.5,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143386442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishing a CRISPR/Cas9 genome editing framework in pigeonpea (Cajanus cajan L.) by targeting phytoene desaturase (PDS) gene disruption","authors":"Kameshwaran Senthil,&nbsp;Maniraj Rathinam,&nbsp;Manisha Parashar ,&nbsp;Narasimham Dokka ,&nbsp;Shaily Tyagi,&nbsp;Vandana Mathur,&nbsp;Sandhya Sharma,&nbsp;Kishor Gaikwad,&nbsp;Ramcharan Bhattacharya,&nbsp;Rohini Sreevathsa","doi":"10.1016/j.jgeb.2025.100465","DOIUrl":"10.1016/j.jgeb.2025.100465","url":null,"abstract":"<div><div>Pigeonpea is an important legume valued for its high nutritional, agricultural, and economic significance in the Asian subcontinent. Despite its potential for high yield, productivity remains stagnant due to several abiotic and biotic stresses. To mitigate these challenges, biotechnological interventions like genome editing offer promising solutions. Towards this, developing a species-specific editing toolkit is crucial for recalcitrant species like pigeonpea. In this study, we established a CRISPR/Cas9 genome editing system targeting the <em>phytoene desaturase</em> (<em>PDS</em>) gene. We developed pigeonpea-compatible vector components, including the <em>Cc</em>U6_7.1 promoter and an amenable Cas9 gene driven by the potato ubiquitin promoter, creating a pigeonpea-specific CRISPR/Cas9 binary vector (PP_CRISPR_pCAMBIA2301). The system was validated by <em>Agrobacterium tumefaciens</em>-mediated apical meristem-targeted <em>in planta</em> and <em>in vitro</em> embryonic axis explant transformations, with gene knockout confirmed by albino/bleached phenotypes. Editing efficiencies were 8.80% and 9.16% in the <em>in planta</em> and <em>in vitro</em> transformations respectively. While PCR analysis confirmed T-DNA integration, sequence analysis identified <em>PDS</em> gene mutations. Stability of the phenotype was demonstrated in T₁ generation plants of <em>in planta</em> transformation-developed mutants. This system may support functional genomics studies and trait improvement in pigeonpea and other legumes.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100465"},"PeriodicalIF":3.5,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143326023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The significant role of IL-15, IL-22, IL-37, and caspase 9 in polycystic ovary syndrome: A case-control study in a sample of Iraqi women","authors":"Noor A. Mohammed ,&nbsp;Ghassan M. Sulaiman ,&nbsp;Hazima M. Alabassi ,&nbsp;Khalil A.A. Khalil ,&nbsp;Elsadig M. Ahmed","doi":"10.1016/j.jgeb.2025.100462","DOIUrl":"10.1016/j.jgeb.2025.100462","url":null,"abstract":"<div><div>The study aims to evaluate the significant role of interleukin 15 (IL-15), IL-22, IL-37, and Caspase 9 gene expression in polycystic ovary syndrome (PCOS), focusing on the underlying mechanisms and potential diagnostic or therapeutic implications. Peripheral blood has been collected, and serum was separated for the evaluation of the serum IL-15, IL-22, and IL-37. The ELISA technique has been carried out to determine the serum levels of understudied factors mentioned above in Iraqi women patients diagnosed with PCOS (No. = 90) via a specialized gynecologist and healthy fertile women (No. = 48) as a control group. In addition, a genetic study on the expression of the caspase 9 gene in these patients had been performed. The data reveals statistically significant differences in interleukin levels in PCOS patients versus the control group. Specifically, the PCOS group exhibits significantly higher levels of IL-15 and IL-22 as compared to the control group<em>.</em> Conversely, the PCOS group shows significantly lower levels of IL-37 compared to the control group. The results showed no statistically significant difference in the mean expression of the Caspase 9 gene when comparing these fold graduations. However, it’s worth noting that a higher fold frequency was observed in both the PCOS and control groups, with 57.1 % and 60 %, respectively, having folds less than 1. The distribution of folds varied across other categories was also addressed. Additionally, there was a notable difference in the frequency of 11.4 % in the PCOS group compared to 2 % in the control group for folds greater than 9. The findings suggest that interleukins, particularly IL-22 and IL-37, hold promise as diagnostic markers for distinguishing PCOS from healthy conditions. However, the potential diagnostic utility of the Caspase 9 gene expression was not confirmed in this study.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100462"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial genetic markers based phylogenetic analyses of Hyalomma dromedarii Koch, 1844 (Acari: Ixodidae)","authors":"Aman D. Moudgil,&nbsp;Anil K. Nehra","doi":"10.1016/j.jgeb.2025.100460","DOIUrl":"10.1016/j.jgeb.2025.100460","url":null,"abstract":"<div><div>The hard tick <em>Hyalomma dromedarii</em>, a vector for numerous animal and human pathogens, was investigated for genetic diversity using the mitochondrial cytochrome <em>C</em> oxidase subunit I (<em>cox I</em>) and 16S ribosomal RNA (<em>16S rRNA</em>) genes. <em>Hyalomma dromedarii</em> sequences (n = 11 <em>cox I</em>; n = 7 <em>16S rRNA</em>) were deposited in GenBank (LC761179-89, LC761173-78, LC654692), showing 99.52–100 % (<em>cox I</em>) and 98.15–100 % (<em>16S rRNA</em>) similarity to existing GenBank sequences. Phylogenetic analysis revealed monophyletic clades for <em>H. dromedarii</em> sequences from this study and GenBank. Haplotype network analysis identified 34 and 11 haplotypes for the <em>cox I</em> and <em>16S rRNA</em> genes, respectively, displaying stellate configurations. The overall <em>cox I</em> dataset showed very low nucleotide diversity (0.0019 ± 0.0002) and high haplotype diversity (0.535 ± 0.052). In contrast, the <em>16S rRNA</em> gene dataset displayed low nucleotide (0.00211 ± 0.00071) and haplotype (0.351 ± 0.079) diversities. Neutrality tests showed significant negative values (Tajima’s D, Fu and Li’s D, and Fu and Li’s F), indicating recent population expansion or selective sweep. Pairwise F_<em>ST</em> values (−0.00942 to 0.02007 for <em>cox I</em>; −0.04878 to 0 for <em>16S rRNA</em>) suggested non-significant genetic differentiation between populations, supported by high gene flow (Nm) values. This study provided novel insights into <em>H. dromedarii</em> population genetics, revealing recent expansion, weak population differentiation, and high gene flow. These findings have implications for understanding the tick’s evolutionary history and epidemiological significance.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100460"},"PeriodicalIF":3.5,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and optimization of in vitro shoot regeneration in Physalis peruviana using cotyledon explants","authors":"Raúl Vargas ,&nbsp;Carmen N. Vigo ,&nbsp;Lily Juarez-Contreras ,&nbsp;Manuel Oliva-Cruz","doi":"10.1016/j.jgeb.2025.100463","DOIUrl":"10.1016/j.jgeb.2025.100463","url":null,"abstract":"<div><div>The genetic improvement of <em>Physalis peruviana</em> L. faces important challenges because it is a recalcitrant species, which limits its use in breeding and conservation programs. In the present research, the objective was to develop an effective regeneration protocol using cotyledon cultures. For this purpose, the effects of zeatin (ZT) and <em>meta</em>-topoline (mT) at concentrations of 0, 0.5, 1.0 and 2.0 mg/L and the effects of several auxins, including indolbutyric acid (IBA), indolacetic acid (IAA), naphthaleneacetic acid (NAA) and 2.4-dichlorophenoxyacetic acid (2.4-D), on morphogenetic responses were evaluated. A maximum regeneration of 62.5 % was achieved with the combination of 2 mg/L ZT, 2 mg/L mT, and 0.5–1 mg/L IBA. For root formation, the best results were obtained with the combination of 2 mg/L ZT, 2 mg/L mT, and 1 mg/L NAA, reaching a maximum rate of 87.5 %. In conclusion, specific combinations of cytokinins and auxins can overcome the resistance of <em>P. peruviana</em> to regeneration and provide a solid basis for biotechnological applications such as genetic transformation and germplasm conservation.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100463"},"PeriodicalIF":3.5,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive in silico genome-wide identification and characterization of SQUAMOSA promoter binding protein (SBP) gene family in Musa acuminata","authors":"Israt Jahan Mouri ,&nbsp;Md. Shariful Islam","doi":"10.1016/j.jgeb.2025.100461","DOIUrl":"10.1016/j.jgeb.2025.100461","url":null,"abstract":"<div><div>One of the largest and most significant transcription factor gene families in plants is the SQUAMOSA promoter binding protein (<em>SBP</em>) gene family and they perform critical regulatory roles in floral enhancement, fruit development, and stress resistance. The <em>SBP</em> protein family (also known as <em>SPL</em>) has not yet been thoroughly studied in the staple fruit crop, banana. A perennial monocot plant, banana is essential for ensuring food and nutrition security. This work detected 41 <em>SBP</em> genes in the banana species <em>Musa acuminata</em>. The <em>MaSBPs</em> were subsequently elucidated by investigating their gene structure, chromosomal position, RNA-Seq data, along with evolutionary connections with Arabidopsis and rice. Sequence alignment of <em>MaSBPs</em> revealed that all genes included a domain of two Zn finger motifs (CCCH and CCHC motifs) with an overlapping nuclear localization signal region. The conserved motifs sequence in the inferred <em>MaSBP</em> proteins were quite comparable. According to findings, the time frame of divergence for duplicated <em>MaSBP</em> gene pairs ranged from 42.39 to 109.11 million years and the dicot Arabidopsis and monocotyledonous plant banana diverged before the division of banana and monocot rice. Moreover, <em>cis</em>-acting element and GO annotation analysis exhibited possible biological activities of <em>MaSBPs</em> in flower development, phytohormone regulation, and stress tolerance. RNA-Seq expression profiling exhibited that genes <em>MaSBP</em>-3, <em>MaSBP</em>-20, <em>MaSBP</em>-37, <em>MaSBP</em>-40 were more expressed during floral and fruit development stage. The foundation for additional investigation of <em>SBP</em> protein sequences in other plants can be laid out by this study, which will shed light on some of their crucial biological functions.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100461"},"PeriodicalIF":3.5,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study on antioxidant properties, total phenolics, total flavonoid contents, and cytotoxic properties of marine green microalgae and diatoms","authors":"Umme Tamanna Ferdous ,&nbsp;Armania Nurdin ,&nbsp;Saila Ismail ,&nbsp;Khozirah Shaari ,&nbsp;Zetty Norhana Balia Yusof","doi":"10.1016/j.jgeb.2024.100456","DOIUrl":"10.1016/j.jgeb.2024.100456","url":null,"abstract":"<div><div>Despite having valuable and novel metabolites, the marine microalgae species are still not thoroughly investigated for their pharmaceutical and nutraceutical importance. Therefore, this study was focused on investigating the crude extracts of marine green microalgae species, <em>Tetraselmis</em> sp., <em>Nannochloropsis</em> sp., and diatoms <em>Chaetoceros</em> sp., and <em>Thalassiosira</em> sp., isolated from the Malaysian coastal region in terms of their antioxidant activity, total phenolics, total flavonoid contents and cytotoxicity against human breast cancer cells, MCF-7. Among twenty-eight crude extracts, <em>Tetraselmis</em> ethanol and ethyl acetate extract showed the highest amount of total phenolic (19.87 mg GAE/g), and total flavonoid content (38.58 mg QE/g of extract), respectively. From the antioxidant assays, methanol and ethyl acetate extract of <em>Tetraselmis</em> sp. exhibited significantly higher (p &lt; 0.05) antioxidant activities, revealed through DPPH (54.41 ± 1.18 mg Trolox Equivalent Antioxidant Capacity or TEAC/g extract) and ABTS (41.57 ± 0.83 mg TEAC/g extract) radical scavenging activities, respectively than the rest. Ethyl acetate extract of <em>Tetraselmis</em> sp. also showed high ferric reducing power (113.46 ± 4.83 mg TEAC/g extract). On the contrary, methanol and ethyl acetate extract of <em>Chaetoceros</em> sp. showed the highest cytotoxicity towards MCF-7 and reduced the cell viability to 21.26 % and 21.56 %, respectively. The data suggest that marine diatom <em>Chaetoceros</em> sp. has a good cytotoxic effect on MCF-7, while marine green microalga <em>Tetraselmis</em> sp. has good radical scavenging and ferric reduction capabilities, warranting further investigation along with their metabolic profiling, cancer cell killing mechanism and extensive <em>in vivo</em> study.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100456"},"PeriodicalIF":3.5,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and genomic evaluation of keratinolytic protease producing Chryseobacterium sp. from tannery waste and its potential application in dehairing of goat skin","authors":"Taslima Akter ,&nbsp;Murshed Hasan Sarkar ,&nbsp;Shashanka Shekhar Sarker ,&nbsp;Nourin Tarannum ,&nbsp;Showti Raheel Naser ,&nbsp;Sanjana Fatema Chowdhury ,&nbsp;Sahana Parveen","doi":"10.1016/j.jgeb.2025.100458","DOIUrl":"10.1016/j.jgeb.2025.100458","url":null,"abstract":"<div><div>Lime and Na₂S, used in dehairing in the tannery industry, cause the generation of toxic wastes. Ecological security and financial issues demand a look for innovative approaches to leather dehairing free from pollution. The primary goal of this investigation was to explore keratinolytic protease producing bacteria from tannery waste, their genomic evaluation and to assess their possible use in the dehairing process. A newly isolated Gram-negative bacterium (LRI-TA6) was characterized and checked for its keratinolytic protease producing ability. The strain was identified as <em>Chryseobacterium cucumeris</em> through whole genome sequencing. The genome was 4,541,898 bp in size and contain 4,426 protein coding sequences (CDS) with 36.38 % of GC content. Twenty-five open reading frames (CDS) were found as protease producing genes notably DegQ (serine protease), DegS, and ATP-dependent protease DP (EC 3.4.21.-). The isolate revealed enzyme production throughout an extended pH range of 5.0 to 8.0, and a broad temperature range of 10 °C to 38 °C. The isolate LRI-TA6 was sensitive to all five antibiotics (amoxicillin, tetracycline, gentamicin, ciprofloxacin, and vancomycin) with zone diameter ranges from 17.2 ± 0.8 to 32 ± 0.0 mm. The enzyme was shown to have 29.9 ± 6.7 and 83.6 ± 0.2 U/ml of keratinolytic and proteolytic activity, respectively. By employing crude keratinase, the removal of hair from goat skin was accomplished in 18 h. This study is the first report to isolate and characterize <em>C. cucumeris</em> from tannery waste and also the amazing 18 h dehairing capabilities and thus might be utilized for further studies towards commercial synthesis of keratinase for use in leather processing sectors.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100458"},"PeriodicalIF":3.5,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}