Journal of Genetic Engineering and Biotechnology最新文献

{"title":"The immunoinfiltration characteristics of LSM4 were investigated using bioinformatics and the biological functions were subsequently confirmed in endometrial carcinoma","authors":"Jianhong Ma, Ling Li, Rui Hu, Yongjuan Ye, Zhiqiang Yao, Ping Sun","doi":"10.1016/j.jgeb.2026.100817","DOIUrl":"10.1016/j.jgeb.2026.100817","url":null,"abstract":"<div><h3>Objective</h3><div>The mounting evidence suggests that the immune status participates in the advancement and metastasis of UCEC as an important element, yet there is currently a dearth of effective and readily detectable biomarkers to reflect this phenomenon. The present study aimed to investigate the expression level, clinical significance, biological function, and immunoinfiltration characteristics of LSM4 in UCEC through bioinformatics analysis. Additionally, cytological experiments were carried out to validate the biological effect of LSM4 and assess its feasibility as a satisfactory endpoint of therapy and prognostic indicator for the patients.</div></div><div><h3>Methods</h3><div>The correlation between LSM4 and 12 clinicopathologic variables was mined using a variety of statistical algorithms. The GO, KEGG, and GSEA enrichment situation were conducted using R software to identify differences and biological functions of different LSM4 gene level, while PPI analysis was realized by the STRING database. The ESTIMATE algorithm was utilized to estimate the TME scores. Furthermore, the role of CIBERSORT was to estimate 22 distinct types of immune infiltrating cells in the tumor immune microenvironment. Kaplan-Meier survival analysis was conducted on DIICs, while the correlation between DIICs and LSM4 expression were demonstrated. The TIMER 2.0 database was utilized to perform online analysis on the correlation between LSM4 and immunoinfiltration. The TISIDB database was utilized to retrieve the association between LSM4 and immune regulatory genes, TCGA molecular typing, immune typing as well as clinical variables. R-package pRRophetic was employed to access GDSC database for analyzing the influence of LSM4 on chemotherapy and targeted drug sensitivities, while GSCALite database was utilized to screen for small molecule drugs related to LSM4. Q-PCR and Western Blot analyses were employed to validate the differential expression of LSM4 in cancer tissues and cells compared to normal conduction. Additionally, CCK8, Transwell and wound healing experiments were performed to ascertain the impression of siLSM4 on biological functions of Ishikawa and KLE cells.</div></div><div><h3>Results</h3><div>In UCEC, the expression of LSM4 is upregulated, which is significantly associated with the deterioration of PFS as well as various adverse clinicopathological features. LSM4 is mainly involved in the regulation of circulating immune complex formation, humoral immunity, classical complement activation pathway, antigen binding. The high expression of LSM4 exhibited a negative correlation with immune score, stromal score, multiple immune cells, GZMA, and most immune cell markers, thereby attenuating the immune activity within the microenvironment. Both immune prediction models further substantiated the involvement of LSM4 in regulating immune infiltration among the patients. Additionally, the presence of high LSM4 expression leads to a reduction in effica","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100817"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149153943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, characterization, and integrated in silico studies of 4-Aminoantipyrine-derived Schiff bases as promising antimicrobial, antioxidant, and cytotoxic agents: DFT, ADME, and molecular docking studies","authors":"Mohammed I. Sultan,&nbsp;Muthanna C. Urabee,&nbsp;Ahmed H. Sadiq,&nbsp;Ahmed Mutanabbi Abdula","doi":"10.1016/j.jgeb.2026.100814","DOIUrl":"10.1016/j.jgeb.2026.100814","url":null,"abstract":"<div><div>This study reports the synthesis, structural characterization, experimental and computational evaluation of a series of Schiff base derivatives (<strong>3a–3f</strong>) obtained from 4-aminoantipyrine via condensation with various aromatic and heteroaromatic aldehydes. The synthesized compounds were fully characterized by FT-IR, ¹H NMR, and mass spectrometry, which showed the formation of preferential imine linkages. The antibacterial activity was evaluated against <em>Staphylococcus aureus</em> and <em>Acinetobacter baumannii</em> using the agar well-diffusion method. All compounds showed significant antibacterial activity, with compound <strong>3a</strong> showing the highest activity against both bacterial strains (IZD = 23 mm and 20 mm, respectively). Antioxidant screening using the DPPH assay showed a wide range of radical scavenging activity, with IC₅₀ values ranging from 56.3 to 423.1 μg/mL, revealing the influence of substituent electronic effects on antioxidant activity. Cytotoxicity evaluation against HepG2 cells showed that compounds 3a, 3e, and 3 f exhibited concentration-dependent cytotoxicity, with compound 3 f showing the highest inhibition (63.03% at 500 μg/mL), while exhibiting low cytotoxicity toward normal Vero cells. DFT calculations performed at the B3LYP/6-311G (d, p) level provided insight into the electronic properties and chemical reactivity of the synthesized compounds, while the Absorption, Distribution, Metabolism, and Excretion (ADME) predictions suggested favorable pharmacokinetic profiles. Molecular docking studies showed strong binding affinities for DNA gyrase (PDB ID: <span><span>4DUH</span><svg><path></path></svg></span>) and VEGFR2 kinase (PDB ID: <span><span>3VHE</span><svg><path></path></svg></span>), with compound <strong>3e</strong> showing the highest binding affinity for DNA gyrase (−8.2 kcal/mol) and compound <strong>3a</strong>, <strong>3e</strong>, and <strong>3</strong> <strong>f</strong> exhibited the highest binding affinities toward VEGFR2 (−10.3 kcal/mol). The determined biological activities were consistent with the docking results, supporting these derivatives as promising multifunctional scaffolds for the development of novel antibacterial and anticancer agents.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100814"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148953448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feeding study of GM Rice bran (Oryza Sativa L. cv Rojolele) overexpressing Bacillus thuringiensis cry1B::cry1Aa fusion genes under the maize ubiquitin promoter in broiler chicken strain cobb","authors":"Andriyanto,&nbsp;Aulia A. Mustika,&nbsp;Amaq Fadholly,&nbsp;Amy Estiati,&nbsp;Dwi Widyajayantie,&nbsp;Enny Rimita Sembiring,&nbsp;Satya Nugroho","doi":"10.1016/j.jgeb.2026.100812","DOIUrl":"10.1016/j.jgeb.2026.100812","url":null,"abstract":"<div><div>Genetically modified (GM) rice (<em>Oryza sativa</em> L) cv Rojolele overexpressing <em>cry1B</em>::<em>cry1Aa</em> fusion genes, driven by the maize <em>Ubiquitin</em> promoter, has been developed to incorporate resistance trait against and to reduce loss due to the rice yellow stem borer (rice YSB, <em>Scirpophaga incertulas</em> Walker). Since rice bran is one of the most commonly used major components in chicken feed, a feeding study of the rice bran of the GM Rojolele rice to the commercial broiler day-old-chick (DOC) strain Cobb was performed. Rice feed compositions were prepared with 6, 8 and 10% GM Rojolele rice bran. Control feed was prepared with non-GM Rojolele rice bran. Nutritional analyses showed no significant differences among the feed compositions. Fifty DOCs were used in each feeding study, treatment and control, and were performed for 30 days. The results indicated that all parameters in the duration evaluated, including total body weight, mortality, feed conversion ratio, hematological responses, liver and kidney functions, and energy digestibility coefficient, exhibited no statistically significant differences between DOCs receiving GM rice bran and those in the control group. The results support the absence of detectable adverse effects within the evaluated endpoints and provide additional information relevant to the feed safety aspects in the regulatory assessment of GM rice-derived feed ingredients.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100812"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148995715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coumarins as modulators of protein–protein interactions: Computational insights, biological mechanisms, and translational perspectives","authors":"Yasser Fakri Mustafa","doi":"10.1016/j.jgeb.2026.100829","DOIUrl":"10.1016/j.jgeb.2026.100829","url":null,"abstract":"<div><div>Coumarins are versatile bioactive scaffolds capable of interacting with diverse biomolecules, yet their potential role in modulating protein–protein complexes remains insufficiently characterized. Modern computational biology now enables rational exploration of how small molecules can influence structural coupling, signal transduction, and pathway rewiring at the interface of proteins. This review aims to synthesize emerging evidence on how integrated computational workflows—combining docking, molecular dynamics, network analysis, and machine-learning—can be leveraged to understand and predict coumarin-driven modulation of protein–protein interactions. Published computational pipelines were surveyed for their application to coumarin–protein complexes, including virtual screening, binding free-energy estimation, structural network modeling, and experimental cross-validation approaches. Mechanistic insights from case studies in signaling, transcriptional regulation, and enzyme assemblies were collated to illustrate biological relevance. Published computational studies suggest that coumarins may engage protein interfaces or PPI-associated regulatory sites through orthosteric or allosteric binding, with predicted consequences for protein conformation and pathway behavior. Across the surveyed literature, simulations predicted ligand-dependent changes involving signaling hubs such as MAPK and JAK/STAT, whereas network-based studies proposed broader changes in interaction landscapes. The strongest evidence derives from selected coumarin-based modulators of the BubR1–CENP-E and LXRα–NCOR1/SMRT complexes, for which computational predictions are supported by biophysical and cellular validation. Unlike conventional reviews that discuss individual computational techniques separately, this review integrates molecular docking, molecular dynamics simulations, network analysis, and machine-learning into a unified computational workflow that facilitates systematic target prioritization, mechanistic interpretation, and rational optimization of coumarin-based protein–protein interaction modulators for subsequent experimental validation. Computational biology provides a powerful lens for unraveling how coumarins influence protein–protein complex behavior at multiple organizational layers. While prediction uncertainties persist, integrative workflows linking structural modeling with systems-level validation offer a promising avenue for transforming coumarins into next-generation chemical probes or therapeutic candidates. Future refinement of data resources, cross-omics integration, and phenotypic readouts may accelerate the translation of <em>in silico</em> predictions into biologically meaningful outcomes.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100829"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149153925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable enhancement of acidic soil fertility through integrated soil amendments: A metagenomic evaluation of fungal functional improvement","authors":"Ireng Darwati,&nbsp;Muchamad Yusron,&nbsp;Tiwit Widowati,&nbsp;Edi Yatno,&nbsp;April Hari Wardhana,&nbsp;Gina Aliyah Sopha,&nbsp;Octivia Trisilawati,&nbsp;Devi Rusmin,&nbsp;Raden Vitri Garvita,&nbsp;Rudi Suryadi,&nbsp;Indarti Puji Lestari,&nbsp;Yati Haryati,&nbsp;Agustina E. Br Marpaung,&nbsp;Muhammad Hikmat,&nbsp;Suryani,&nbsp;Tika Anggraeni","doi":"10.1016/j.jgeb.2026.100823","DOIUrl":"10.1016/j.jgeb.2026.100823","url":null,"abstract":"<div><div>Acid soil is characterized by low pH, which restricts nutrient availability and disrupts overall soil biological functioning. Soil ameliorants, such as <em>Hermetia illucens</em> (black soldier fly; BSF) frass, lime, and biochar, are widely applied to restore the fertility of acidic soils by improving both physicochemical and biological properties. This study evaluated the dynamics of the fungal community in acidic Typic Hapludult soil under three conditions: untreated soil, BSF frass, and ameliorant-treated soil. For each condition, 1000 g of material was placed in plastic containers and incubated at room temperature for three months. The ameliorant-treated condition consisted of 5 g BSF frass, 5 g lime, 2.5 g homogenized arbuscular mycorrhizal fungi inoculum, and 987.5 g soil. Fungal communities were characterized using Illumina-based next-generation sequencing, while soil chemical parameters were analyzed by spectrophotometry and atomic absorption spectrometry. Metabarcoding analysis provides novel evidence that ameliorant application restructured fungal communities in acidic soils and changed soil chemical properties. Order Onygenales and families Aspergillaceae, Chaetomiaceae, and Terramycetaceae correlated positively with soil pH, base cations (K⁺, Na⁺, Ca²⁺, and Mg²⁺), and soil organic carbon, and negatively with Al³⁺. In contrast, families Microascaceae and Saccharomycetaceae correlated positively with Al³⁺ and available phosphorus, and negatively with base cations and cation exchange capacity. The findings highlight the potential of combining biological and organic ameliorants as a sustainable soil amelioration strategy to enhance fungal community activity.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100823"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149153942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomic analysis of Wolbachia strain wCin2USA1 reveals two pairs of cytoplasmic incompatibility factor (cif) genes and multiple intact prophage regions","authors":"Istiaque Zaeem,&nbsp;Nurnabi Azad Jewel,&nbsp;Daniyal Karim,&nbsp;Mohimenul Haque Rolin,&nbsp;Arzuba Akter,&nbsp;Shakhinur Islam Mondal","doi":"10.1016/j.jgeb.2026.100822","DOIUrl":"10.1016/j.jgeb.2026.100822","url":null,"abstract":"<div><div>Dengue fever poses an escalating global public health challenge, causing substantial morbidity and economic burden, particularly in tropical and subtropical regions. <em>Wolbachia</em>-mediated biocontrol has emerged as a cost-effective and environmentally sustainable strategy to suppress dengue transmission. However, the vulnerability of currently deployed <em>Wolbachia</em> strains in <em>Ae. aegypti</em> underscores the need to explore novel strains with enhanced stability and efficacy. This study aimed to identify alternative <em>Wolbachia</em> candidates for dengue control through comparative genomic analyses of eight <em>Wolbachia pipientis</em> strains: wMel, wAlbB, wlrr, wHm<img>C, wCin2USA1, wAnm, ant7, and a wMel variant (wMel_Lab) isolated from <em>Ae. aegypti</em>. Comprehensive analyses were conducted, including phylogenetic reconstruction, metabolic pathway profiling, and detailed characterization of Cytoplasmic Incompatibility Factor (Cif) genes. Among the strains examined, wCin2USA1 was distinguished by its cif gene and prophage complement. This strain exhibited substantial genomic similarity to wMel (98% average nucleotide identity), the strain currently used in<em>Ae. aegypti</em> release programs. Notably, wCin2USA1 harbored two distinct pairs of Cif genes from different monophyletic clades, homologous to those found in wMel (Type I) and wAlbB (Type III), respectively. Both pairs are syntenic and comprise uninterrupted open reading frames. This architecture is of a type associated with cytoplasmic incompatibility in other characterized strains, although the CI phenotype of wCin2USA1 itself remains experimentally untested. Additionally, seven prophage regions were identified in wCin2USA1, indicating genomic plasticity; notably, none of the cif loci was located within a predicted prophage region. Collectively, these findings characterize the genomic architecture of an understudied <em>Wolbachia</em> strain and identify wCin2USA1 as warranting experimental investigation; its potential as a biocontrol agent remains a prospect contingent on functional validation. Future studies should prioritize transinfection into Ae. aegypti, crossing and embryonic hatch-rate assays to establish CI phenotype, compatibility testing against wMel- and wAlbB-infected lines, and dengue virus challenge assays.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100822"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149043767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic silver and zinc oxide nanoparticles synthesized using Foeniculum vulgare seed extract: Antimicrobial activity and in vivo toxicological evaluation","authors":"Saman Muhsin Abdulkareem,&nbsp;Sabir Ahmed Ismael,&nbsp;Youns Saleh Mustafa,&nbsp;Sarwar Nawzad Jafar,&nbsp;Rebwar Muhammad Hamasalih,&nbsp;Muhsin Ali Ahmed,&nbsp;Zirak Faqe Ahmed Abdulrahman","doi":"10.1016/j.jgeb.2026.100832","DOIUrl":"10.1016/j.jgeb.2026.100832","url":null,"abstract":"<div><div>Biogenic synthesis of metal nanoparticles using medicinal plants has emerged as a sustainable and environmentally friendly strategy for producing multifunctional nanomaterials with promising biomedical applications. Here, an aqueous seed extract of <em>Foeniculum vulgare</em> was utilized as a natural reducing and stabilizing agent for the synthesis of silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnONPs). The synthesized nanoparticles were characterized by UV–Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). UV–Visible analysis confirmed nanoparticle formation through characteristic surface plasmon resonance and absorption peaks, while FTIR spectra indicated the involvement of plant-derived phytochemicals in nanoparticle reduction and stabilization. XRD analysis verified the crystalline structure of both nanomaterials, with an average crystallite size of approximately 37 nm, whereas SEM revealed predominantly spherical nanoparticles with diameters ranging from 22 to 128 nm. EDX analysis further confirmed the elemental composition of the synthesized AgNPs and ZnONPs.</div><div>The antimicrobial performance of the biogenic nanoparticles was investigated against <em>Staphylococcus aureus</em>, <em>Pseudomonas aeruginosa</em>, and <em>Candida albicans</em> using agar well diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal/fungicidal concentration (MBC/MFC) assays. Both AgNPs and ZnONPs exhibited concentration-dependent antimicrobial activity; however, AgNPs produced larger inhibition zones and lower MIC and MBC/MFC values than ZnONPs. Among the tested microorganisms, <em>C. albicans</em> was the most susceptible, whereas <em>P. aeruginosa</em> exhibited the highest level of resistance.</div><div>The in vivo toxicological profile was evaluated following oral administration of 10 mg/kg/day for 14 consecutive days. AgNP-treated animals showed only minor biochemical and histopathological alterations. In contrast, ZnONP treatment significantly increased serum ALT, AST, and urea levels and significantly decreased ALP activity and the hepato-somatic index compared with the control group (<em>P</em> &lt; 0.05). ZnONP-treated animals also exhibited more pronounced hepatic and renal histopathological alterations than AgNP-treated animals. These findings indicate that <em>F. vulgare</em>-mediated AgNPs provide superior antimicrobial performance with a more favorable short-term safety profile than ZnONPs under the experimental conditions. Collectively, the results highlight the potential of biogenic AgNPs synthesized from <em>F. vulgare</em> seed extract as promising antimicrobial nanomaterials for future biomedical and pharmaceutical applications.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100832"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149199826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum zonulin levels and their association with inflammatory and oxidative stress biomarkers in patients undergoing maintenance hemodialysis","authors":"Ali B. Roomi,&nbsp;Hussein Jawad Kadhim,&nbsp;Abbas Khairi Fadhil,&nbsp;Ibrahim Abdullah Hamzah","doi":"10.1016/j.jgeb.2026.100842","DOIUrl":"10.1016/j.jgeb.2026.100842","url":null,"abstract":"<div><h3>Background</h3><div>Maintenance hemodialysis (HD) is accompanied by persistent inflammation, oxidative stress, and disturbances of the intestinal barrier.</div></div><div><h3>Objective</h3><div>To compare serum zonulin concentrations between patients receiving maintenance HD and healthy controls and to evaluate their associations with interleukin-6 (IL-6), malondialdehyde (MDA), and total antioxidant status (TAS).</div></div><div><h3>Methods</h3><div>This comparative cross-sectional study included 180 participants: 90 patients with end-stage renal disease receiving maintenance HD and 90 age- and sex-comparable healthy controls. Predialysis blood samples were analyzed for serum zonulin, IL-6, and MDA by enzyme-linked immunosorbent assay and for TAS by a colorimetric assay. Group differences were evaluated using independent-samples <em>t</em>-tests, associations using Pearson's correlation, and adjusted relationships using multivariable linear regression.</div></div><div><h3>Results</h3><div>Compared with controls, the HD group had higher serum zonulin, IL-6, MDA, urea, creatinine, and phosphorus concentrations and lower TAS, albumin, calcium, and hemoglobin values (all <em>P</em> &lt; 0.001). Within the HD group, zonulin correlated positively with IL-6 (<em>r</em> = 0.644, <em>P</em> &lt; 0.001) and MDA (<em>r</em> = 0.782, <em>P</em> &lt; 0.001) and inversely with TAS (<em>r</em> = −0.392, <em>P</em> &lt; 0.001). After covariate adjustment, zonulin remained associated with IL-6 (B = 0.982, β = 0.577, <em>P</em> &lt; 0.001), MDA (B = 36.76, β = 0.597, <em>P</em> &lt; 0.001), and TAS (B = −0.018, β = −0.345, <em>P</em> = 0.021).</div></div><div><h3>Conclusion</h3><div>Serum zonulin was elevated in patients receiving maintenance HD and was independently associated with inflammatory and oxidative-stress biomarkers. These cross-sectional findings support an association but do not establish that altered intestinal permeability causes inflammatory-oxidative imbalance or that serum zonulin is ready for clinical use.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100842"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149199827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a cryopreservation system and evaluation of post-thaw effects for Piao chicken primordial germ cells","authors":"Yifan Xi,&nbsp;Fufu Cheng,&nbsp;Qingqing Geng,&nbsp;Zhe Wang,&nbsp;Kunyu Liang,&nbsp;Jing Chen,&nbsp;Xinyue Jiao,&nbsp;Junqiang Hou,&nbsp;Yani Zhang","doi":"10.1016/j.jgeb.2026.100805","DOIUrl":"10.1016/j.jgeb.2026.100805","url":null,"abstract":"<div><div>Primordial germ cell (PGC) cryobanking provides an alternative strategy for conserving endangered chicken genetic resources; however, evidence regarding the post-thaw stability of breed-specific PGCs remains limited. This study evaluated a stepwise two-solution cryopreservation procedure based on CO₂-independent medium for gonad-derived Piao chicken PGCs stored for 3, 6, 9, and 12 months. Post-thaw characteristics were assessed by morphological observation, periodic acid–Schiff staining, trypan blue exclusion, EdU incorporation, CCK-8 analysis, JC-1 staining, quantitative reverse transcription PCR, immunofluorescence, flow cytometry, and embryonic gonadal homing analysis. Cryopreserved PGCs retained typical round or oval morphology and remained periodic acid–Schiff positive after thawing. Post-thaw mortality remained low (approximately 4–5%). No statistically significant differences were detected in mortality, proliferation-associated or metabolic parameters, or the expression of the evaluated genes (<em>P</em> &gt; 0.05). JC-1 fluorescence patterns were qualitatively similar among the evaluated groups. DDX4 and SSEA-1 protein signals remained detectable after cryopreservation, and the embryo-level gonadal-homing-positive proportions were 70% in the Fresh group and 78% in the Frozen 12 M group, with no statistically significant difference between the two groups. These findings provide preliminary evidence that selected cellular and molecular characteristics were retained during the evaluated 12-month storage period. Larger biological sample sizes, quantitative assessment of donor-cell number and distribution within individual gonads, germline transmission testing, and genomic and epigenetic stability assessments are still required.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100805"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148856004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PI3K/AKT-associated fibroblast activation by native Argiope anasuja spidroin for cutaneous wound healing: An in vitro approach","authors":"Sri Raagavee Sivakumar,&nbsp;Kamarajan Rajagopalan,&nbsp;Rajendran Venkatesh,&nbsp;Jackson Durairaj Selvan Christyraj,&nbsp;P. Susheela,&nbsp;Shobana Sampath,&nbsp;Douglas Law,&nbsp;Gholamreza Abdi","doi":"10.1016/j.jgeb.2026.100820","DOIUrl":"10.1016/j.jgeb.2026.100820","url":null,"abstract":"<div><div>Spidroins, the main structural protein constituents of spider silk, show excellent mechanical strength, durability and biocompatibility; therefore, they are potentially useful biomaterials for medical uses. Nevertheless, the molecular mechanisms that underlie their wound-healing activity are not well understood. The present study has been designed to investigate the in vitro wound-healing potential of the Native <em>Argiope anasuja</em> orb-web spidroin protein in NIH/3T3 fibroblasts and the underlying molecular mechanisms. Cell viability was measured by MTT assay, and fibroblast migration and re-epithelialization were determined by scratch wound assay under 0% FBS, 10% FBS, 1× PBS vehicle control and 5% spidroin treatment. In the spidroin-treated group, fibroblast migration was enhanced, and wound closure was complete within 36 h when compared to the control groups. Quantitative real-time PCR (qRT-PCR) analysis demonstrated an increased in VEGFR2, PI3K and AKT gene expression in spidroin-treated cells, indicating the activation of angiogenic and proliferative signalling pathways. Western blot analysis also verified activation of the PI3K/AKT signalling pathway, as indicated by an 8.5-fold increase in the expression of PI3K, a 1.2-fold increase in the expression of AKT, and a 2.8-fold increase in the expression of phosphorylated AKT (p-AKT) compared with controls. Immunofluorescence studies also revealed increased fluorescence intensity of VEGFR2, PI3K and AKT in the treated cells. In total, these results indicate that native <em>Argiope anasuja</em> spidroin induces fibroblast proliferation and migration through involvement of VEGFR2-associated PI3K/AKT signalling pathways, which illustrates its therapeutic potential in wound healing and tissue regeneration.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100820"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149043754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}