Journal of Genetic Engineering and Biotechnology最新文献

{"title":"Screening and genomic evaluation of keratinolytic protease producing Chryseobacterium sp. from tannery waste and its potential application in dehairing of goat skin","authors":"Taslima Akter ,&nbsp;Murshed Hasan Sarkar ,&nbsp;Shashanka Shekhar Sarker ,&nbsp;Nourin Tarannum ,&nbsp;Showti Raheel Naser ,&nbsp;Sanjana Fatema Chowdhury ,&nbsp;Sahana Parveen","doi":"10.1016/j.jgeb.2025.100458","DOIUrl":"10.1016/j.jgeb.2025.100458","url":null,"abstract":"<div><div>Lime and Na₂S, used in dehairing in the tannery industry, cause the generation of toxic wastes. Ecological security and financial issues demand a look for innovative approaches to leather dehairing free from pollution. The primary goal of this investigation was to explore keratinolytic protease producing bacteria from tannery waste, their genomic evaluation and to assess their possible use in the dehairing process. A newly isolated Gram-negative bacterium (LRI-TA6) was characterized and checked for its keratinolytic protease producing ability. The strain was identified as <em>Chryseobacterium cucumeris</em> through whole genome sequencing. The genome was 4,541,898 bp in size and contain 4,426 protein coding sequences (CDS) with 36.38 % of GC content. Twenty-five open reading frames (CDS) were found as protease producing genes notably DegQ (serine protease), DegS, and ATP-dependent protease DP (EC 3.4.21.-). The isolate revealed enzyme production throughout an extended pH range of 5.0 to 8.0, and a broad temperature range of 10 °C to 38 °C. The isolate LRI-TA6 was sensitive to all five antibiotics (amoxicillin, tetracycline, gentamicin, ciprofloxacin, and vancomycin) with zone diameter ranges from 17.2 ± 0.8 to 32 ± 0.0 mm. The enzyme was shown to have 29.9 ± 6.7 and 83.6 ± 0.2 U/ml of keratinolytic and proteolytic activity, respectively. By employing crude keratinase, the removal of hair from goat skin was accomplished in 18 h. This study is the first report to isolate and characterize <em>C. cucumeris</em> from tannery waste and also the amazing 18 h dehairing capabilities and thus might be utilized for further studies towards commercial synthesis of keratinase for use in leather processing sectors.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100458"},"PeriodicalIF":3.5,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Process optimization and extraction of alkaline protease from halotolerant Streptomyces sp. VITGS3 and its use as a contact lens cleaner","authors":"Gargi Sarkar,&nbsp;K. Prem Anand,&nbsp;M.A. Jayasri,&nbsp;K. Suthindhiran","doi":"10.1016/j.jgeb.2025.100459","DOIUrl":"10.1016/j.jgeb.2025.100459","url":null,"abstract":"<div><div>Marine halotolerant actinobacteria are robust microbes poorly explored and barely cultivable in nature. They are a trove of various secondary metabolites and enzymes, especially the alkaline proteases withstanding higher temperatures, pH, and salinity, making them an ideal source with versatile commercial and therapeutic values. This study focuses on extracting and optimizing alkaline protease production from <em>Streptomyces</em> sp. VITGS3 isolated from Puthuvypeen, Kerala. The protease production was optimized by Response Surface Methodology (RSM) using the Box-Behnken model, which used rice bran, wheat bran, skim milk, and casein as substrates. The maximum protease was produced (357 U/mL) using wheat bran (5.5 % w/v) as substrate at pH 9 and incubated at 45 °C for 9 days. The Michaelis-Menten model’s enzyme kinetics exhibited a K_m value of 1.42 µM, a V_max of 201.64 µM·min⁻¹, V₀ of 5.59 µM·min⁻¹, and K_cat 70013.89 min⁻¹ suggesting a higher affinity of the enzyme for the substrate (1 % w/v casein). In addition, the protease was inhibited by the phenylmethylsulphonyl fluoride (PMSF), suggesting it belongs to the serine protease family. Finally, the application studies as contact lens cleaners showcased that the isolated protease effectively degraded the protein deposits present in the artificial tear solution without affecting the light transmittance. This is a milestone in the implication of protease on therapeutic applications and further studies on protein specificity, sustained releases, and combination strategies, resulting in crucial challenges in long-term studies, cross-reactivity, storage, and cost-effectiveness.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100459"},"PeriodicalIF":3.5,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the molecular activity of HIV towards the CD4: A study based on subtype C via docking and dynamics approach","authors":"Saurav Kumar Mishra ,&nbsp;Neeraj Kumar ,&nbsp;Zsolt Tóth ,&nbsp;Yousef A. Bin Jardan ,&nbsp;Shopnil Akash ,&nbsp;John J. Georrge","doi":"10.1016/j.jgeb.2025.100457","DOIUrl":"10.1016/j.jgeb.2025.100457","url":null,"abstract":"<div><h3>Background</h3><div>Acquired Immunodeficiency Syndrome (AIDS) is a critical global health issue caused by the human immunodeficiency virus (HIV). It has different strains and subtypes; among these, Subtype C accounts for higher infection rates than others. Despite its high prevalence, the molecular interactions with host receptors, specifically CD4, have not yet been explored.</div></div><div><h3>Methods</h3><div>This study investigates the molecular interactions between HIV subtype C and the CD4 receptor via docking and dynamics approach. Four HIV targets were examined, and their structure was modelled. Subsequently, these models were docked with the CD4 to analyze their binding interaction. The stability was examined over 200 simulations via Desmond software, and trajectories were analyzed, followed by Root mean square deviation (RMSD), root mean square fluctuation (RMSF), and the radius of gyration (Rg), PCA (principal component analysis), etc., to assess their stability and interaction dynamics.</div></div><div><h3>Results</h3><div>The four target structures were modelled, and their quality was validated. Further, the docking analysis with CD4 revealed that the Envelope glycoprotein has −13.6 kcal/mol, protease has −11.2 kcal/mol, Reverse transcriptase has −12.4 kcal/mol, and integrase has −13.1 kcal/mol binding affinity towards it, followed by the number of hydrogen bond, such as 9, 6, 11, 6. The simulation over 200 ns demonstrated that the average RMSD for each complex started stabilizing within the 0.9 Å − 3.4 Å, followed by 25–50 ns, whereas the RMSF, Rg and PCA revealed the relative compactness and flexibility varied across different viral targets.</div></div><div><h3>Conclusions</h3><div>The study successfully identified the interactive residues of HIV subtype C toward the CD4 receptor. The binding affinities and stability data provide valuable insights into Subtype C’s molecular interactions with the host, and these findings underscore the potential for developing treatments that disrupt these interactions to combat HIV more effectively.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100457"},"PeriodicalIF":3.5,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Telomeric RNA quadruplexes as targets for cancer prevention: The therapeutic potential of agonodepsides","authors":"Gourav Choudhir ,&nbsp;Sushil Kumar ,&nbsp;Mohammad Shahid ,&nbsp;Anas Shamsi ,&nbsp;Asimul Islam","doi":"10.1016/j.jgeb.2024.100454","DOIUrl":"10.1016/j.jgeb.2024.100454","url":null,"abstract":"<div><h3>Background</h3><div>Cancer remains an awful challenge, despite years of targeting proteins to control its relentless growth and spread. Fungal metabolites, a treasure of natural chemicals, offer a glimmer of hope. Telomeres, the cellular “caps,” are a focal point in cancer research. This study explores the potential of stabilizing Telomeric Repeats-containing RNA G-quadruplex (TERRA G4) structures within telomeres. This stabilization could block telomerase, the enzyme that repairs telomeres, and potentially trigger cancer cell death. Agonodepsides A and B, two promising fungal metabolites, were chosen to investigate this exciting possibility.</div></div><div><h3>Methods</h3><div>Agonodepside A and B were initially screened for drug likeness employing SwissAdme. AutoDock Vina was used for molecular docking, and ligands and TERRA G4 were prepared using PyRx and MGL tool. Discovery Studio software was utilized for the visualization of interactions between ligands and TERRA G4. For validation of docking results MD simulation for control and complexes was carried out for 250 ns and trajectories were analyzed for different parameters. MMPBSA was used to calculate binding free energy for control and complexes. To find the stable and lower energy states of complexes in comparison to control principal component analysis (PCA) and free energy landscape (FEL) were conducted.</div></div><div><h3>Results</h3><div>Absorption, distribution, metabolism, and excretion (ADME) of both agonodepsides followed Lipinski’s rule of five with zero violation. Molecular docking revealed several key interactions including hydrogen bonds, van der Waals interactions, π-alkyl and π-anion. MD simulation revealed that Agonodepside A interact with TERRA G4 and stabilize it while Agonodepside B interactions were transient. The MMPBSA binding free energy calculation, PCA and free energy landscapes supported the docking and MD simulation results.</div></div><div><h3>Conclusion</h3><div>Lichenized fungi produce agonodepsides A and B, may fight cancer by targeting telomeres. Agonodepside A binds more strongly to telomeres than B, potentially blocking enzyme telomerase. Further studies are required to validate these findings and evaluate potential safety concerns.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100454"},"PeriodicalIF":3.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput screening of natural antiviral drug candidates against white spot syndrome virus targeting VP28 in Penaeus monodon: Computational drug design approaches","authors":"Md. Touki Tahamid Tusar ,&nbsp;Zubaer Hossen ,&nbsp;Hafizur Rahman Gazi ,&nbsp;Niamul Haq ,&nbsp;Abdullah-Al Jubayer ,&nbsp;Md Mahmudul Islam ,&nbsp;Asura Khanam Lisa ,&nbsp;Biswanath Sikdar ,&nbsp;Md. Enamul Haque","doi":"10.1016/j.jgeb.2024.100455","DOIUrl":"10.1016/j.jgeb.2024.100455","url":null,"abstract":"<div><div>The white spot syndrome virus (WSSV), considered the deadliest pathogen impacting Penaeid shrimp (<em>Penaeus monodon</em>), remains worrisome for the global shrimp industry due to its extreme virulence and mortality rate of up to 100%. To date, there has been no breakthrough in effective antivirals or vaccines that can mitigate the financial damage caused by the pathogen. The distinctive structure of VP28 facilitates its role as a trimer, serving as the primary envelope protein of WSSV. It anchors to the viral envelope, directly interacts with PmRab7, a membrane protein in <em>P. monodon</em>, and aids in entry into the host. This research aims to discover antiviral drug candidates targeting VP28 trimer by screening a virtual library of 187 bioactive compounds derived from the medicinal herbs <em>Azadirachta indica</em> and <em>Bacopa monnieri</em>. To evaluate the drug ability of compounds in restricting VP28 trimer interaction within the endocytic pathway, a computational strategy was employed, including virtual screening, pharmacokinetics and toxicity analysis, and molecular dynamics (MD) simulation. The four strongest compounds, epicatechin, luteolin, kaempferol, and apigenin, exhibited binding affinities of −8.8, −8.8, −8.7, and −8.5 Kcal/mol, respectively, and demonstrated excellent pharmacokinetic properties. Furthermore, we employed 100 nanoseconds MD simulations and MM-PBSA binding free energy calculations to examine intermolecular interactions and confirmed the structural stability of the compounds at the VP28 binding site. The findings of this research suggest that these compounds hold promise in combating WSSV infection, reducing economic losses, and contributing to the sustainability of the shrimp industry.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100455"},"PeriodicalIF":3.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of crucial stromal risk genes associated with prognostic significance and chemotherapeutic opportunities in invasive ductal breast carcinoma","authors":"Guohua Tang ,&nbsp;Zhi Wang ,&nbsp;Wei Geng ,&nbsp;Yang Yu ,&nbsp;Yang Zhang","doi":"10.1016/j.jgeb.2024.100448","DOIUrl":"10.1016/j.jgeb.2024.100448","url":null,"abstract":"<div><h3>Background</h3><div>Few studies revealed that stromal genes regulate the tumor microenvironment (TME). However, identification of key-risk genes in the invasive ductal breast carcinoma-associated stroma (IDBCS) and their associations with the prediction of risk group remains lacking.</div></div><div><h3>Methods</h3><div>This study used the GSE9014, GSE10797, GSE8977, GSE33692, and TGGA BRCA datasets. We explored the differentially expressed transcriptional markers, hub genes, gene modules, and enriched KEGG pathways. We employed a variety of algorithms, such as the log-rank test, the LASSO-cox model, the univariate regression model, and the multivariate regression model, to predict prognostic-risk genes and the prognostic-risk model. Finally, we employed a molecular docking-based study to explore the interaction of sensitive drugs with prognostic-risk genes.</div></div><div><h3>Results</h3><div>In comparing IDBCS and normal stroma, we discovered 1472 upregulated genes and 1400 downregulated genes (combined ES &gt; 0585 and adjusted p-value &lt; 0.05). The hub genes enrich cancer, immunity, and cellular signaling pathways. We explored the 12 key risk genes (<em>ADAM8, CD86, CSRP1, DCTN2, EPHA1, GALNT10, IGFBP6, MIA, MMP11, RBM22, SLC39A4,</em> and <em>SYT2</em>) in the IDBCS to identify the high-risk group and low-risk group patients. The high-risk group had a lower survival rate, and the constructed ROC curves evaluated the validity of the risk model. Expression validation and diagnostic efficacy revealed that the key stromal risk genes are consistently deregulated in the high-risk group and high stromal samples of the TCGA BRCA cohort. The expression of crucial risk genes, including <em>CD86, CSRP1, EPHA1, GALNT10, IGFBP6, MIA,</em> and <em>RBM22</em> are associated with drug resistance and drug sensitivity. Finally, a molecular docking study explored several sensitive drugs (such as QL-XII-61, THZ-2-49, AZ628, NG-25, lapatinib, dasatinib, SB590885, and dabrafenib) interacted with these essential risk genes through hydrogen bonds and other chemical interactions.</div></div><div><h3>Conclusions</h3><div>Exploring essential prognostic-risk genes and their association with the prognosis, diagnostic efficacy, and risk-group prediction may provide substantial clues for targeting the breast cancer stromal key-risk genes.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100448"},"PeriodicalIF":3.5,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic analysis of Gobiopterus brachyopterus (Oxudercidae) from Grati Lake and The genus potential misidentification","authors":"Septi Anitasari ,&nbsp;Diana Arfiati ,&nbsp;Susilo Susilo ,&nbsp;Agung Pramana Warih Marhendra ,&nbsp;Abdul Rahem Faqih ,&nbsp;Akhsan Fikrillah Paricahya","doi":"10.1016/j.jgeb.2024.100453","DOIUrl":"10.1016/j.jgeb.2024.100453","url":null,"abstract":"<div><div>The holotype of <em>Gobiopterus brachyopterus</em> originates from Grati Lake, Indonesia. It is locally called lempuk fish. The genus <em>Gobiopterus</em> has similar species, making identification difficult. A genetic study using the COI region revealed possible misidentification issues. Phylogenetic analysis showed that populations in India and Brunei are separate species. These populations formed distinct clades from the Grati Lake population. Genetic P-distance supported this, with a 21.7% difference for India and 21.9% for Brunei. A haplotype network confirmed the Grati Lake population’s uniqueness. It is genetically closer to <em>Gobiopterus lacustris</em> than to Indian or Brunei populations. <em>G. brachyopterus</em> was found to be genetically endemic to Grati Lake.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100453"},"PeriodicalIF":3.5,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive evaluation of antibacterial and anticancer activities from indole butanoic acid","authors":"Azhar H. Ali ,&nbsp;Mohanad Yakdhan Saleh ,&nbsp;Qusay Abdulazahra Yaqoob ,&nbsp;Shakir M. Saied ,&nbsp;Mohammed Sami Hasan ,&nbsp;Khalid Ahmed Owaid ,&nbsp;Basma A.A. Balboul ,&nbsp;Heba.G. Abdelzaher ,&nbsp;M.A. Abdelzaher ,&nbsp;Alaa Muqbil Alsirhani","doi":"10.1016/j.jgeb.2024.100452","DOIUrl":"10.1016/j.jgeb.2024.100452","url":null,"abstract":"<div><div>Focus of this study is on the use of the hydrazone compound (3) (N-(4-bromobenzylidene)-4-(1H-indol-3-yl) butane hydrazide), which was previously prepared from the reaction of the compound p-bromobenzaldehyde with the corresponding hydrazide (2), as an intermediate compound for the synthesis of azetidine, thiazolidine, tetrazole, oxadiazole and phthalazine heterocyclic compounds through its reaction with some cyclic reagents and catalysts such as chloro acetyl chloride, thioglycolic acid, sodium-azid, lead dioxide and Hydrogen chloride gas. The prepared compounds were characterized using physical properties and also spectroscopic methods such as infrared spectroscopy, nuclear magnetic resonance spectra of the proton and the isotope of carbon¹³ as well as mass spectrometry, which accurately identified the proposed structures of the prepared compounds. The identity of the prepared compounds was determined using physical and spectroscopic properties, where infrared and ¹HNMR spectroscopy of the proton as well as carbon¹³ were used in addition to using mass spectrometry to verify the validity of the prepared structures. Conclusions: Also, the biological antibacterial evaluation of the compounds (4–8) was conducted and it gave a good result compared to the drug (8) used as a reference for the control, The MTT test was performed on the healthy and cancerous cells of the compounds (4,5,8) and gave an excellent result for the compound (8).</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100452"},"PeriodicalIF":3.5,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Algal L– asparaginase: Antioxidant activity, mitigation of acrylamide in fried potato chips, sensory quality and immobilization","authors":"Hanaa Abd El Baky ,&nbsp;Gamal El Baroty","doi":"10.1016/j.jgeb.2024.100450","DOIUrl":"10.1016/j.jgeb.2024.100450","url":null,"abstract":"<div><h3>Background</h3><div>Several microalgae and macro-algae have been showed considerable promise bio-material in various multidisciplinary fields. l-asparaginase (l- ASase) have a greater reduction effect on the formation of acrylamide in heated carbohydrate food products such as potato chips and bakery produced at high temperatures (above 120 °C). Acrylamide showed neurotoxic and carcinogenic effects in experimental animals and humans. The immobilized of l-asparaginase (l-ASase) in chitosan nanoparticles have used as a strategy to produce efficient and efficacious biocatalysts.</div></div><div><h3>Result</h3><div>L-asparaginase (l-ASase) extracted by 1-butyl 3-methyl imidazolium chlorideionic liquid (IL, 0.2 mmol/L) reagent from five macro and 3-micro-algae species was evaluated for its scavenging radical activity and its application (ranges of 0.5 IU − 2.0 IU) for reduction of acrylamide (ACA) content in raw potato chips prior to the fried at 170 °C for 8 min. The isolated algae (l-ASase) showed a scavenging activity toward DPPH radical, in effective dose dependent manner and pre treated of slits of potatoes causes a high reduction effects in ACA contents (&gt;88 %) in potato chips products. These products showed a good sensory quality (texture and acceptability). l-ASase of <em>Spirulina platensis</em> was chosen to immobilized into chitosane, which showed a higher enzyme yield (90 %) and enzyme activity as compared to the free enzyme. The pretreatment of potatoes with immobilized l-ASase of <em>Spirulina platensis</em> causes high reduction of ACA formation in potato chips products.</div></div><div><h3>Conclusion</h3><div>It was concluded that the pre-treated of potato’s with chitosan-immobilized asparaginase is an effective method for mitigation of acrylamide. The higher affinity immobilized l-ASase on chitosan was confirmed, and could be a applied as a cost-effective tool for subsequent use in the therapeutic and in heat food industries sectors.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100450"},"PeriodicalIF":3.5,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of Dual-Color FISH probes targeting 9p21, Xp21, and 17p13.1 loci as diagnostic markers for some genetic disorders and cancer in Egypt","authors":"Amal M. Mohamed,&nbsp;Maha Eid,&nbsp;Ola Eid,&nbsp;Shymaa H Hussein,&nbsp;Wael Mahmoud,&nbsp;Rana Mahrous,&nbsp;Khaled Rafaat,&nbsp;Marwa Farid","doi":"10.1016/j.jgeb.2024.100449","DOIUrl":"10.1016/j.jgeb.2024.100449","url":null,"abstract":"<div><h3>Introduction</h3><div>The fluorescence in situ hybridization (FISH) is a very important technique, as it can diagnose many genetic disorders and cancers. Molecular cytogenetic analysis (FISH) can diagnose numerical chromosome aberrations, sex chromosomes anomalies, and many genetic disorders.</div></div><div><h3>Aim</h3><div>With the limited number of commercially available probes that do not cover all research needs and the high prices of the commercial probes, our goal is to apply recent technologies to produce FISH probes that can accurately and sensitively diagnose genetic diseases and cancer in Egypt and establishing the inhouse production of different FISH probes. We intend to adhere to the published guidelines and validation procedures to ensure the production of accurate FISH probes for clinical diagnosis.</div></div><div><h3>Methods</h3><div>We used specific DNA segments extracted from BAC clones, and we performed nick translation to label the segment with fluorescence labeled dye. The second method involved the use of specific primers for the centromere of certain chromosomes and using PCR technique for amplification and labeling. The probes were tested on metaphase and interphase cells derived from cultured human peripheral blood samples. We followed standard guidelines to test the adequacy of probe slide hybridization, proper probe localization, probe sensitivity and specificity, probe reproducibility, cut-off values, and overall probe validation.</div></div><div><h3>Results</h3><div>In this research, we presented the generation of three dual-color probes, each probe has a control locus. We offered three dual-color probes targeted 9p21, Xp21 and 17p13.1 loci. chromosome 9p21probe for diagnosis of structural abnormalities in chromosome 9, the Xp21 to test for structural abnormalities of chromosome X, and the 17p13.1 for TP53 gene to detect the loss of p53. We also produced probes for Down syndrome specific region, Rb gene and centromeres for chromosomes X, 17, and 18.</div></div><div><h3>Conclusion</h3><div>The produced probes are specific and sensitive and can be produced at the commercial level in the laboratory. The production of FISH probes in Egypt can be used as a powerful diagnostic marker for genetic disorders and cancers and our work can be consider as a base to start national project to produce our needs of FISH probes.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 1","pages":"Article 100449"},"PeriodicalIF":3.5,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}