Journal of Genetic Engineering and Biotechnology最新文献

{"title":"Analytical and biological assessment of Magnolia champaca L. stem bark: Integrating ATR-FTIR, GC–MS, thrombolytic activity, brine shrimp lethality and molecular docking","authors":"Md. Mahadi Hasan , As-Sazzad Mahmud Nishan , Most. Humayra Binta Rashid , Bijoy Chandra Ghos , Jaytirmoy Barmon","doi":"10.1016/j.jgeb.2025.100505","DOIUrl":"10.1016/j.jgeb.2025.100505","url":null,"abstract":"<div><div>Cancer and cardiovascular disease are two of the most significant health challenges worldwide. These diseases are responsible for morbidity and mortality, leading to a substantial burden on healthcare systems and economic resources. Numerous innovative therapies have been proposed for combating cancer and cardiovascular disease like thrombosis; however, many of these treatments involve synthetic drugs that may have undesirable side effects. Consequently, the current research aimed to explore the medicinal properties of phytochemicals derived from <em>Magnolia champaca</em> L. This investigation utilized both <em>in vitro</em> and <em>in silico</em> approaches. The identification of phytocompounds were employed by using ATR-FTIR and GC–MS. Thrombolytic activity was assayed using human venous blood and cytotoxicity was assessed through lethality test using nauplii of <em>Artemia salina</em> and finally the molecular docking analysis was performed using a docking software named AutoDock Vina to clearly understand about the interactions of GC–MS identified secondary metabolites with target proteins. The ATR-FTIR spectrum of <em>Magnolia champaca</em> specifies the presence of some alkaloids, polysaccharides, flavonoids as well as glycosidic phytocompounds and GC–MS data identified 23 compounds from crude methanolic extract and among them 2,4-Di-<em>tert</em>-butylphenol; 3-Ethyl-3-hydroxyandrostan-17-one; Hexadecanoic acid, methyl ester; Di-n-octyl phthalate were prominent. The <em>in vitro</em> thrombolytic assay demonstrated that Trans-Syringin and CHF resulted in significant blood clot lysis, achieving 59.39 ± 1.79 % and 45.66 ± 3.63 % respectively comparing 75.75 ± 2.96 % with standard streptokinase. The brine shrimp bioassay indicated moderate to strong toxicological effect. Specifically, Trans-Syringin and the ethyl acetate fraction (EAF) demonstrated considerable cytotoxicity, showing the net values of LC<sub>50</sub> were 22.39 µg/ml and 61.78 µg/ml, respectively contrary to 11.45 µg/ml of vincristine sulfate (standard). Molecular docking analysis were conducted to explore the synergistic effects of the compounds identified through GC–MS analysis on tissue plasminogen activator and human topoisomerase-II. The compounds 3-Ethyl-3-hydroxyandrostan-17-one; Cadina-1(10),4-diene and 1,1,7-Trimethyl-4-methylenedecahydro-1H-cyclopropa[e]azulen-7-ol-, (1aR-(1a.alpha.,4a.alpha.,7.beta.,7a.beta.,7b.alpha.))- demonstrated high binding affinities of −7.7, −7.3, and −7.1 kcal/mol, respectively, for tissue plasminogen activator where streptokinase (standard) had −6.5 kcal/mol. For human topoisomerase-II, 3-Ethyl-3-hydroxyandrostan-17-one; Caryophyllene-(I1) and Cadina-1(10),4-diene exhibited binding affinities of −8.0, −7.9 as well as −7.8 kcal/mol correspondingly, while standard vincristine had −8.1 kcal/mol. These findings suggest that <em>Magnolia champaca</em> contains active phytochemicals with significant potentials for diverse pharmacologi","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 2","pages":"Article 100505"},"PeriodicalIF":3.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association study −Driven drug repositioning for the treatment of insomnia","authors":"Rahmat Dani Satria ,&nbsp;Wirawan Adikusuma ,&nbsp;Usi Sukorini ,&nbsp;Devy Eka Avitasari ,&nbsp;Indra Sari Kusuma Harahap ,&nbsp;Mohammad Hendra Setia Lesmana ,&nbsp;Lalu Muhammad Irham ,&nbsp;Nur Imma Fatimah Harahap ,&nbsp;Pranindya Rinastiti ,&nbsp;Donytra Arby Wardhana ,&nbsp;Richardus Wisnandito Prabasaktya ,&nbsp;Chiou-Feng Lin ,&nbsp;Aprilia Paramitasari ,&nbsp;Andrian Fajar Kusumadewi","doi":"10.1016/j.jgeb.2025.100502","DOIUrl":"10.1016/j.jgeb.2025.100502","url":null,"abstract":"<div><div>Insomnia is a prevalent sleep disorder characterized by difficulty initiating or maintaining sleep, leading to severe health complications, increased mortality, and substantial socioeconomic burdens. Despite therapeutic advancements, effective pharmacological interventions remain limited, necessitating alternative approaches for drug discovery. This study aimed to identify potential therapeutic targets for insomnia by integrating gene network analysis, genomic data, and bioinformatics-driven drug repurposing strategies, aligning with the United Nations’ Sustainable Development Goal (SDG) 3: Good Health and Well-being. Insomnia-associated Single Nucleotide Polymorphisms (SNPs) were retrieved from the GWAS catalog, yielding 3,952 loci. Insomnia risk genes were identified by linking these loci to proximal SNPs (r² ≥ 0.8) in Asian populations using HaploReg v4.2, resulting in 1,765 candidate genes. A bioinformatics pipeline incorporating ten functional annotations and drug-gene interaction was employed to prioritize gene targets and identify novel repurposed drugs with potential biological relevance to insomnia. Drug-Gene Interaction Database (DGIdb) analysis identified seven druggable targets among 27 biologically significant insomnia risk genes, corresponding to 12 existing drugs. Notably, <em>NRXN1</em> emerged as a highly promising target due to its strong functional annotation score and its known interaction with Duloxetine hydrochloride and nicotine polacrilex. This study underscores the potential of bioinformatics-driven gene network analysis in identifying drug repurposing candidates for insomnia. Further experimental validation is warranted to elucidate the therapeutic mechanisms of NRXN1 modulation in insomnia treatment.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 2","pages":"Article 100502"},"PeriodicalIF":3.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Derived Artificial neural networks predict PRRC2A as a potent biomarker for epilepsy","authors":"Wayez Naqvi,&nbsp;Prekshi Garg,&nbsp;Prachi Srivastava","doi":"10.1016/j.jgeb.2025.100503","DOIUrl":"10.1016/j.jgeb.2025.100503","url":null,"abstract":"<div><div>Epilepsy refers to the occurrence of two or more than two reiterative seizures. The occurrence of seizure is governed by the excessive electrical discharges in the cortex of the brain. Bioinformatics is crucial in diagnosing, prognosticating, and treating neurological disorders. It uses methodologies, computational tools, software, and databases to probe disease molecular underpinnings and identify biomarkers. It aids clinicians in addressing patient parameters and translational research. Artificial neural networks (ANNs) are computer models that attempt to mimic the neurons present in the human brain. This computerized neuronal model is used for analyzing and comprehending large and complex data sets. In the present study, three GEO datasets (GSE190451, GSE140393, and GSE134697) were retrieved from NCBI for the identification of differentially expressed genes using the DESeq2 package. The study identified 7 up-regulated genes (PRRC2A, FCGR3B, HLA-DRB, ENSG00000280614, ENSG00000281181, SLN, C4A) in patients with epilepsy. Furthermore, WEKA software was used for feature selection and classification of DEGs using feature selection algorithms namely Correlation Feature Selection, ReliefF, and Information Gain and classification methods such as Logistic regression, Classification via regression, Random forest, Random subspace, and Logistic model trees. After the analysis, out of the 7 genes, the C4A gene was removed as it yielded the lowest feature selection statistics. Lastly, R Studio was used for constructing the Artificial Neural Network of the 6 identified DEGs. The model’s performance was evaluated using the “pROC” R package, and an AUC of 0.720 was obtained, indicating that the model had excellent classification accuracy. The NeuralNet package of R revealed that PRRC2A had the highest generalized weight value indicating the increased expression of these genes when all other parameters are constant. Therefore, PRRC2A can be used as a potential biomarker for the diagnosis of epilepsy.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 2","pages":"Article 100503"},"PeriodicalIF":3.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anther-mediated haploid induction and genetic stability of Phytophthora-resistant backcross lines of Capsicum annuum L","authors":"Surender Kumar ,&nbsp;Aayushee Thakur ,&nbsp;Munish Sharma ,&nbsp;Neha Thakur ,&nbsp;Sanjeev Kumar ,&nbsp;Anupama Singh","doi":"10.1016/j.jgeb.2025.100498","DOIUrl":"10.1016/j.jgeb.2025.100498","url":null,"abstract":"<div><div>Bell pepper is an important vegetable crop from <em>Solanaceae</em> family, which is cultivated and consumed globally due to its delicious, pleasant and nutrient-rich nature. Most of the commercially cultivated bell pepper cultivars are however, highly susceptible to <em>P. capsici</em>. They are recalcitrance to <em>in vitro</em> regeneration, which make the crop improvement efforts difficult through plant tissue culture-based approaches such as double haploid production. In this study, <em>in vitro</em> callus regeneration protocol was established from anthers of two <em>Phytophthora</em> resistant-backcross lines derived from crossing of two highly susceptible bell pepper cultivars (California Wonder and Solan Bharpur) with a highly resistant chilli landrace, CM334.The cultures were initially established in full strength MS medium, supplemented with different concentration of auxin and cytokinin. Maximum callus induction was achieved in MS medium CI2 supplemented with IAA (2 mg/L) and BAP (0.3 mg/L) (41.59 %) at pH 5.8, without heat-shock and cold stress treatments. The light-green and white compact calli were obtained which proliferated and maintained in the half strength MS medium containing 0.1 mg/L Kinetin (pH 5.8). The presence of <em>Phytophthora</em> resistance loci and successful crossings were confirmed through PCR amplification with the closely linked (RGA-C) and pungency −specific (Pun2) molecular markers, and phenotypic evaluations against <em>P. capsici</em>. The haploid nature and genetic stability were revealed through squash staining with 2 % acetocarmin. This study standardized the conditions appropriate for double haploid culture and regeneration of whole plants to speed up the development of <em>Phytophthora</em>-resistant bell pepper varieties.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 2","pages":"Article 100498"},"PeriodicalIF":3.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of genetic diversity and elucidation of population structure of Lathyrus accessions using agro-morphological characters and ISSR technique","authors":"Reda Helmy Sammour ,&nbsp;Abd EL-Zaher Mohammed Abasery Mustafa ,&nbsp;Hanan Fahad Al-Harbi ,&nbsp;Mohamed Ragab Abdelgawwad ,&nbsp;Fatin Khalid AlShamasi","doi":"10.1016/j.jgeb.2025.100500","DOIUrl":"10.1016/j.jgeb.2025.100500","url":null,"abstract":"<div><div>Genetic diversity and population structure were evaluated for 36 <em>Lathyrus L</em>. accessions using agronomic characters and ISSR markers. The agronomic characters and ISSR markers showed a wide genetic diversity between <em>Lathyrus</em> accessions. The significant association between 1000 seed weight and total seed protein percentage in <em>Lathyrus</em> accessions revealed that both characters were under independent genetic control. The total number of alleles generated by the markers was 336, revealing 100% polymorphism. The polymorphic information content (PIC), effective multiplex ratio (EMR), marker index (MI), and resolving power (RP) of the markers were 0.75, 16.46, 17.83, and 2.70, respectively, confirming the great variation in <em>Lathyrus</em> accessions. The AMOVA test indicated that 93% of all genetic variation was found within accessions and 7% between accessions. The Ewens–Watterson test for neutrality indicated that the nine markers were neutral, and most of the genetic diversity resided in accessions, with the exception of <em>Lathyrus aphaca</em> from Bulgaria, confirming AMOVA results. The genetic diversity (<em>h</em>) and Shannon’s information index were 0.34 and 0.52, respectively, revealing that maker OPA01-812 was highly recommended for distinguishing <em>Lathyrus</em> accessions. The genetic differentiation among accessions (<em>G_ST</em>) was 0.03, whereas the number of migrants per generation based on Wright’s equation (<em>N_mw</em>) was 18.12. However, the probability (P) of the chi-square values exceeded 0.05 for the nine markers, indicating the low heterogeneity between the investigated accessions. Cluster and correspondence analyses for ISSR results showed that the genetic diversity among <em>Lathyrus</em> accessions was not influenced by their geographical origin. They also revealed that <em>Lathyrus sativus</em> and <em>Lathyrus cicera</em> were not derived from the same ancestor. In conclusion, we recommended (1) <em>Lathyrus sativus</em> PI 179299 for breeding for new cultivars with a high yield and low β-ODAP percentage and (2) ISSR markers for characterizing and differentiating between <em>Lathyrus</em> accessions.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 2","pages":"Article 100500"},"PeriodicalIF":3.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TIMELESS as a prognostic biomarker and therapeutic target in gastric cancer","authors":"Xiangrong Meng ,&nbsp;Qi Sun ,&nbsp;Zhongshuang Liu ,&nbsp;Shenqi Cao ,&nbsp;Chunyang Xu ,&nbsp;Longjiang Di ,&nbsp;Yan Wu ,&nbsp;Wenjing Zhang","doi":"10.1016/j.jgeb.2025.100504","DOIUrl":"10.1016/j.jgeb.2025.100504","url":null,"abstract":"<div><h3>Background</h3><div>Gastric cancer (GC), one of the most prevalent malignancies worldwide, is characterized by complex etiological and pathological mechanisms. Emerging evidence on the dysregulation of circadian clock genes has revealed promising opportunities for improving the diagnosis, treatment, and prognosis of patients with GC. Methods: This study utilized a multifaceted approach combining machine learning algorithms, gene set enrichment analysis, immune infiltration profiling, survival prognosis analysis, drug sensitivity testing, and <em>in vitro</em> experiments to investigate the functional roles of core clock genes in GC. Results: By integrating data from The Cancer Genome Atlas, Gene Expression Omnibus datasets, and the National Center for Biotechnology Information database, we identified 29 differentially expressed clock genes in GC. Among these, the application of four distinct machine learning algorithms highlighted TIMELESS (TIM) and BHLHE41 as pivotal genes, with TIM demonstrating notable diagnostic performance (area under the receiver operating characteristic curve = 0.802). Elevated TIM expression was strongly associated with poor clinical prognosis and increased infiltration of immune cells in tumor tissues. Notably, a specific interaction was identified between TIM and the pyroptosis-associated molecule CASP8, indicating a potential synergistic role in GC pathogenesis. Additionally, bortezomib emerged as a potential targeted therapeutic agent capable of modulating TIM activity in GC. Conclusion: TIM is identified as a promising diagnostic biomarker and therapeutic target in GC, offering valuable implications for improving patient prognosis and guiding personalized treatment strategies.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 2","pages":"Article 100504"},"PeriodicalIF":3.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of ionizing radiation and low-energy electrons on DNA functionality: radioprotection and radiosensitization potential of natural products","authors":"Kouass Sahbani Saloua ,&nbsp;Rayan M. Alansari","doi":"10.1016/j.jgeb.2025.100501","DOIUrl":"10.1016/j.jgeb.2025.100501","url":null,"abstract":"<div><div>Ionizing radiation (IR) is a key cancer treatment, but its DNA-damaging effects, particularly double-strand breaks (DSBs) and clustered lesions, pose challenges for therapy. Clustered DNA lesions, often induced by low-energy electrons (LEEs), contribute significantly to genomic instability and repair resistance. Chemotherapeutic agents like cisplatin can enhance IR-induced damage, making tumor cells more susceptible. Emerging strategies in radiation oncology target DNA repair pathways, using inhibitors like poly(ADP-ribose) polymerase (PARP) to sensitize tumors to IR. Natural products, including polyphenols, flavonoids, and alkaloids, offer promising radioprotective effects by scavenging reactive oxygen species and enhancing DNA repair. These agents not only protect normal tissues but also increase tumor sensitivity to IR, improving therapeutic outcomes. Future research should focus on optimizing these natural agents for clinical use, integrating them into radiotherapy protocols for enhanced efficacy and reduced toxicity.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 2","pages":"Article 100501"},"PeriodicalIF":3.5,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of silver nanoparticles using Padina commersonii: Characterization, hypoglycemic effects, and antimicrobial potential against human pathogenic bacteria","authors":"R. Ragavi ,&nbsp;P. Sachini Sigera ,&nbsp;A.A.E.B. Nirmani ,&nbsp;Milan Rathnayake ,&nbsp;R.A. Pasindu Eranga ,&nbsp;K.K. Dinuhara Theekshana ,&nbsp;Dinithi C. Peiris","doi":"10.1016/j.jgeb.2025.100493","DOIUrl":"10.1016/j.jgeb.2025.100493","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Nanotechnology is a dynamic field comprising chemistry, physics, biology, and engineering, with a high proficiency in disease treatment delivery methods. Metallic precursors (e.g.,AgNO&lt;sub&gt;3&lt;/sub&gt;) are widely used to synthesize NPs. But, owing to their harmful nature and the cost of these metallic nanoparticles, the focus is shifting toward “green synthesis“ of nanoparticles using fungi, bacteria, plants, and algae. Hence, we used &lt;em&gt;Padina commersonii&lt;/em&gt; to green synthesize NPs. We focused on the antioxidant, hypoglycemic, and antimicrobial efficacy of biologically synthesized AgNPs from &lt;em&gt;P&lt;/em&gt;. &lt;em&gt;commersonii.&lt;/em&gt;&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;Silver NPs (AgNPs) were synthesized using 80% methanol extract of &lt;em&gt;P. commersonii&lt;/em&gt; upon removal of pigments and polysaccharides. Methanol (80%) was selected for higher polyphenol yield (∼30% w/w) compared to aqueous extracts (∼12% w/w), critical for efficient Ag&lt;sup&gt;+&lt;/sup&gt; reduction. Major phytochemicals, including fucoidan, phlorotannins, flavonoids (quercetin), and terpenes, are present in 80% methanol extract of &lt;em&gt;P. Commersonii&lt;/em&gt; The NPs were characterized using ultraviolet–visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, dynamic light scattering, energy dispersive X-ray (X-ray diffraction (XRD), and scanning electron microscopy (SEM). Antioxidant potential was determined using DPPH and ABTS scavenging assays, while hypoglycemic activity was conducted using alpha-glucosidase and alpha-amylase enzyme inhibitory assays. Finally, antimicrobial activities were conducted against S. aureus, E. &lt;em&gt;coli&lt;/em&gt; (bacteria), and the fungal strains &lt;em&gt;Candida albicans&lt;/em&gt; and &lt;em&gt;Aspergillus niger&lt;/em&gt;.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;The AgNPs were confirmed by the colour change from yellow to brown. Further, the UV and Raman spectrophotometry peaks, respectively, at 424 nm and 335 cm&lt;sup&gt;-1&lt;/sup&gt;, confirmed the presence of AgNPs. The average AgNPs size of 73.19 nm was obtained using the dynamic light scattering&lt;!--&gt; &lt;!--&gt;(DLS) technique, while the zeta potential indicated its stability at −21.5 mV. The SEM imaging confirmed a smooth surface with no aggregation, whereas the XRD pattern perceived the crystalline nature of these AgNPs. The antioxidant potential of AgNPs was higher than (DPPH: IC&lt;sub&gt;50&lt;/sub&gt;: 271.17 ± 3.992 and ABTS: IC50 195.03 ± 5.7535 µg/mL) the methanol extract. Similar patterns were observed with the hypoglycemic potential with α-glucosidase (IC50:161.74 ± 3.992 µg/mL) and α-amylase inhibitions (IC50: 176.70 ± 5.7535 µg/mL). The antimicrobial efficacy of AgNPs was evident for studied bacterial species (S.aureus:12.77 ± 0.58 mm; E.coli:15.27 ± 0.58 mm) and fungi (A. niger: 18.10 ± 0.15 mm; C.albicans: 17.4 ± 0.57 mm).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;The AgNPs displayed potent antidiabetic and antimicrobial efficacy, suggesting their potential as viable alternatives to conventional ","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 2","pages":"Article 100493"},"PeriodicalIF":3.5,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evaluation of a novel multi-epitope vaccine against human papillomavirus and Candida albicans","authors":"Fatemeh Ahmadifar ,&nbsp;Ebrahim Balali ,&nbsp;Ramona Khadivi ,&nbsp;Mehrdad Hashemi ,&nbsp;Ali Jebali","doi":"10.1016/j.jgeb.2025.100489","DOIUrl":"10.1016/j.jgeb.2025.100489","url":null,"abstract":"<div><h3>Purpose</h3><div>The purpose of this study was to design and evaluate a multiepitope peptide vaccine against Human papillomavirus (<em>HPV</em>) and <em>Candida albicans</em> (<em>C. albicans</em>) on human dendritic cells (<em>hDC</em>).</div></div><div><h3>Methods</h3><div>To determine the antigenic epitopes for <em>C. albicans</em> and <em>HPV</em>, the FASTA format of protein targets was extracted from NCBI. Then, all epitopes were analyzed using ABCpred, Vaxijen, AllerTOP, ToxinPred, and molecular docking. Then, selected peptides were first synthesized using the solid phase method, mixed with cationic lipids, and characterized by DLS, Zetasizer, and nanodrop at 280 nm. In the next step, <em>hDC</em>s were obtained from peripheral blood and confirmed by flow cytometry. Then, they were exposed to vaccine candidates at 1 mg/mL. Next, the activation of <em>hDC</em> was checked by the expression and concentration of interleukin-12 (IL-12) and interferon-ɣ (IFN-ɣ). Also, the functionality of <em>hDC</em>s and antigen-loaded <em>hDC</em>s was evaluated by their ability to induce the proliferation of allogeneic T cells by mixed lymphocyte reaction (MLR). Finally, antibody titration against vaccine candidates was investigated on Syrian male mice using the ELISA method.</div></div><div><h3>Results</h3><div>Here, KDIDLDLQEL, GPVLALNVAL, and KDIDLDLQELKKKGPVLALNVAL peptides were selected as E7, HYR1, and merged E7-HYR1 peptides, respectively. We found that more than 85 % of the isolated <em>hDC</em>s were positive for HLA-DR, CD86, and CD209 and less than 5 % were positive for CD14. After treatment of <em>hDC</em>s with vaccine candidates, we found that peptides E7, HYR1, and merged E7-HYR1 could stimulate <em>hDC</em>s to express IL-12 and IFN-ɣ and when the selected peptides integrated into nanoliposomes, they were significantly more capable to stimulate <em>hDC</em> (P &lt; 0.05). Also, we found that the stimulation index of vaccine-loaded <em>hDC</em>s was higher than unloaded <em>hDC</em>s. The antibody titer in mice exposed to nanoliposomes was significantly higher than in mice exposed to specific peptide vaccines. ^a P &lt; 0.05 compared with other groups.</div></div><div><h3>Conclusion</h3><div>Here, a multiepitope peptide vaccine against <em>HPV</em> and <em>C. albicans</em> was designed and evaluated on <em>hDC</em>. Real efficacy and side effects of this vaccine must be analyzed in future studies.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 2","pages":"Article 100489"},"PeriodicalIF":3.5,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-expression of HIF1A with multi-drug transporters (P-GP, MRP1, and BCRP) in chemoresistant breast, colorectal, and ovarian cancer cells","authors":"Sudipta Deb Nath ,&nbsp;Md Tamzid Hossain Tanim ,&nbsp;Md. Mahmudul Hasan Akash ,&nbsp;Mohammad Golam Mostafa ,&nbsp;Abu Ashfaqur Sajib","doi":"10.1016/j.jgeb.2025.100496","DOIUrl":"10.1016/j.jgeb.2025.100496","url":null,"abstract":"<div><div>Therapeutic resistance poses a significant challenge in treating most cancers and often leads to poor clinical outcomes and even treatment failure. One of the primary mechanisms that confer multidrug resistance phenotype to cancer cells is the hyperactivity of certain drug efflux transporters. P-GP, MRP1, and BCRP are the key ABC efflux pumps that collectively extrude a broad spectrum of chemotherapeutic drugs. Besides, HIF1A, a master transcription regulatory protein, is also associated with cancer development and therapeutic resistance. Thereby, this study aimed to delve into the mechanisms of drug resistance, specifically focusing on HIF1A-driven overexpression of ABC transporters. A total of 57 chemoresistant and 57 paired control tissue samples (breast, colorectal, and ovarian) from Bangladeshi cancer patients were analyzed to determine the co-expression level of ABC transporters and HIF1A. Molecular docking was also conducted to evaluate the interactions of HIF1A protein and hypoxia response element (HRE) sequences in the promoter regions transporter genes. This study revealed that HIF1A is significantly overexpressed in chemoresistant tissues, suggesting its pivotal role in chemoresistance mechanisms across malignancies and its potential as a target to overcome therapeutic resistance. The findings from this study also suggest a direct upregulation of <em>ABCB1</em>, <em>ABCC1</em>, and <em>ABCG2</em> transcription by HIF1A in chemoresistant cancer cells by binding to the HRE sequence in the promoter regions. Thus, inhibition of these interactions of HIF1A appears to be a promising approach to reverse chemoresistance. The findings of this study can serve as a foundation for future research, resolving molecular intricacies to improve treatment outcomes in chemoresistant patients.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 2","pages":"Article 100496"},"PeriodicalIF":3.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}