Marine Life Science & Technology最新文献_第3页

Genome-resolved metagenomics of Venice Lagoon surface sediment bacteria reveals high biosynthetic potential and metabolic plasticity as successful strategies in an impacted environment 威尼斯泻湖表层沉积物细菌的基因组解析宏基因组学揭示了在受影响的环境中具有高生物合成潜力和代谢可塑性的成功策略

2区生物学

Marine Life Science & Technology Pub Date : 2023-11-03 DOI: 10.1007/s42995-023-00192-z

Elisa Banchi, Erwan Corre, Paola Del Negro, Mauro Celussi, Francesca Malfatti

{"title":"Genome-resolved metagenomics of Venice Lagoon surface sediment bacteria reveals high biosynthetic potential and metabolic plasticity as successful strategies in an impacted environment","authors":"Elisa Banchi, Erwan Corre, Paola Del Negro, Mauro Celussi, Francesca Malfatti","doi":"10.1007/s42995-023-00192-z","DOIUrl":"https://doi.org/10.1007/s42995-023-00192-z","url":null,"abstract":"Abstract Bacteria living in sediments play essential roles in marine ecosystems and deeper insights into the ecology and biogeochemistry of these largely unexplored organisms can be obtained from ‘omics’ approaches. Here, we characterized metagenome-assembled-genomes (MAGs) from the surface sediment microbes of the Venice Lagoon (northern Adriatic Sea) in distinct sub-basins exposed to various natural and anthropogenic pressures. MAGs were explored for biodiversity, major marine metabolic processes, anthropogenic activity-related functions, adaptations at the microscale, and biosynthetic gene clusters. Starting from 126 MAGs, a non-redundant dataset of 58 was compiled, the majority of which (35) belonged to (Alpha- and Gamma-) Proteobacteria. Within the broad microbial metabolic repertoire (including C, N, and S metabolisms) the potential to live without oxygen emerged as one of the most important features. Mixotrophy was also found as a successful lifestyle. Cluster analysis showed that different MAGs encoded the same metabolic patterns (e.g . , C fixation, sulfate oxidation) thus suggesting metabolic redundancy. Antibiotic and toxic compounds resistance genes were coupled, a condition that could promote the spreading of these genetic traits. MAGs showed a high biosynthetic potential related to antimicrobial and biotechnological classes and to organism defense and interactions as well as adaptive strategies for micronutrient uptake and cellular detoxification. Our results highlighted that bacteria living in an impacted environment, such as the surface sediments of the Venice Lagoon, may benefit from metabolic plasticity as well as from the synthesis of a wide array of secondary metabolites, promoting ecosystem resilience and stability toward environmental pressures.","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"33 10","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135818875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mucosal immune responses to Ichthyophthirius multifiliis in the ocular mucosa of rainbow trout (Oncorhynchus mykiss, Walbaum), an ancient teleost fish 虹鳟(Oncorhynchus mykiss, Walbaum)眼黏膜对多filiis鱼的免疫反应。虹鳟是一种古老的硬骨鱼

2区生物学

Marine Life Science & Technology Pub Date : 2023-10-31 DOI: 10.1007/s42995-023-00199-6

Weiguang Kong, Guangyi Ding, Gaofeng Cheng, Peng Yang, Zhen Xu

{"title":"Mucosal immune responses to Ichthyophthirius multifiliis in the ocular mucosa of rainbow trout (Oncorhynchus mykiss, Walbaum), an ancient teleost fish","authors":"Weiguang Kong, Guangyi Ding, Gaofeng Cheng, Peng Yang, Zhen Xu","doi":"10.1007/s42995-023-00199-6","DOIUrl":"https://doi.org/10.1007/s42995-023-00199-6","url":null,"abstract":"Abstract The eye, as a specialized visual organ, is directly exposed to the external environment, and, therefore, it faces constant challenges from external pathogenic organisms and toxins. In the ocular mucosa (OM) of mammals, mucosal-associated lymphoid tissues (MALTs) constitute the primary line of defense. However, the immune defense role of the OM remains unknown in aquatic vertebrates. To gain insights into the immune processes within the OM of teleost fish, we developed an infection model of rainbow trout ( Oncorhynchus mykiss ) OM using a parasite, Ichthyophthirius multifiliis (Ich). Immunofluorescence, qPCR, and H&E staining revealed that Ich successfully infiltrates the OM of rainbow trout, leading to pathological structural changes, as evidenced by A&B staining. Importantly, the qPCR results indicate an up-regulation of immune-related genes following Ich infection in the OM. Moreover, transcriptome analyses were conducted to detect immune responses and impairments in eye function within the OM of rainbow trout with Ich infection. The results of the transcriptome analysis that Ich infection can cause an extensive immune response in the OM, ultimately affecting ocular function. To the best of our knowledge, our findings represent for the first time that the teleost OM could act as an invasion site for parasites and trigger a strong mucosal immune response to parasitic infection.","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"161 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135869927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional evidence that FGFR regulates MAPK signaling in organizer specification in the gastropod mollusk Lottia peitaihoensis FGFR在腹足类软体动物中调控MAPK信号传导的功能证据

2区生物学

Marine Life Science & Technology Pub Date : 2023-10-21 DOI: 10.1007/s42995-023-00194-x

Sujian Tan, Pin Huan, Baozhong Liu

引用次数: 0

Linking coral fluorescence phenotypes to thermal bleaching in the reef-building Galaxea fascicularis from the northern South China Sea 南海北部造礁星系中珊瑚荧光表型与热白化的关联

2区生物学

Marine Life Science & Technology Pub Date : 2023-10-18 DOI: 10.1007/s42995-023-00190-1

Sanqiang Gong, Jiayuan Liang, Gang Li, Lijia Xu, Yehui Tan, Xinqing Zheng, Xuejie Jin, Kefu Yu, Xiaomin Xia

{"title":"Linking coral fluorescence phenotypes to thermal bleaching in the reef-building Galaxea fascicularis from the northern South China Sea","authors":"Sanqiang Gong, Jiayuan Liang, Gang Li, Lijia Xu, Yehui Tan, Xinqing Zheng, Xuejie Jin, Kefu Yu, Xiaomin Xia","doi":"10.1007/s42995-023-00190-1","DOIUrl":"https://doi.org/10.1007/s42995-023-00190-1","url":null,"abstract":"Abstract Coral fluorescence phenotypes have been suggested as an adaptation to a broad range of environmental conditions, yet the mechanisms linking thermal bleaching tolerance in reef-building coral populations, associated with fluorescence phenotypes due to GFP-like proteins, remains unclear. In this study, the relationship between the thermal sensitivity and phenotypic plasticity of corals was investigated using two phenotypes of Galaxea fascicularis, green and brown. The results reveal that brown G. fascicularis was more susceptible to bleaching than green G. fascicularis when exposed to a higher growth temperature of 32 °C. Both phenotypes of G. fascicularis were associated with the thermotolerant Symbiodiniaceae symbiont, Durusdinium trenchii . However, the brown G. fascicularis showed a significant decrease in Symbiodiniaceae cell density and a significant increase in pathogenic bacteria abundance when the growth temperature was raised from 29 to 32 °C. The physiological traits and transcriptomic profiles of Symbiodiniaceae were not notably affected, but there were differences in the transcriptional levels of certain genes between the two phenotype hosts of G. fascicularis. Under heat stress of 32 °C, the gene encoding green fluorescent protein (GFP)-like and chromosome-associated proteins, as well as genes related to oxidative phosphorylation, cell growth and death showed lower transcriptional levels in the brown G. fascicularis compared to the green G. fascicularis. Overall, the results demonstrate that the green form of G. fascicularis is better able to tolerate ocean warming and defend against pathogenic bacteria, likely due to higher gene transcription levels and defense ability.","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"243 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135825228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards disentangling the classification of freshwater fish trypanosomes 淡水鱼锥虫分类的厘清

2区生物学

Marine Life Science & Technology Pub Date : 2023-10-13 DOI: 10.1007/s42995-023-00191-0

Peng Zhang, Jie Liu, Xiao-Ming Yin, Jun-Yu Zhou, Julius Lukeš, Zhao-Rong Lun, De-Hua Lai

引用次数: 1

Correction: Incorporating mesopelagic fish into the evaluation of marine protected areas under climate change scenarios 更正:将中上层鱼类纳入气候变化情景下海洋保护区的评估

2区生物学

Marine Life Science & Technology Pub Date : 2023-10-13 DOI: 10.1007/s42995-023-00193-y

Shuhao Liu, Yang Liu, Katharina Teschke, Mark A. Hindell, Rachel Downey, Briannyn Woods, Bin Kang, Shuyang Ma, Chi Zhang, Jianchao Li, Zhenjiang Ye, Peng Sun, Jianfeng He, Yongjun Tian

引用次数: 0

Contribution of the SOS response and the DNA repair systems to norfloxacin induced mutations in E. coli SOS反应和DNA修复系统对诺氟沙星诱导的大肠杆菌突变的贡献

2区生物学

Marine Life Science & Technology Pub Date : 2023-09-21 DOI: 10.1007/s42995-023-00185-y

Tongtong Lin, Jiao Pan, Colin Gregory, Yaohai Wang, Clayton Tincher, Caitlyn Rivera, Michael Lynch, Hongan Long, Yu Zhang

{"title":"Contribution of the SOS response and the DNA repair systems to norfloxacin induced mutations in E. coli","authors":"Tongtong Lin, Jiao Pan, Colin Gregory, Yaohai Wang, Clayton Tincher, Caitlyn Rivera, Michael Lynch, Hongan Long, Yu Zhang","doi":"10.1007/s42995-023-00185-y","DOIUrl":"https://doi.org/10.1007/s42995-023-00185-y","url":null,"abstract":"Abstract Antibiotic-resistant bacteria severely threaten human health. Besides spontaneous mutations generated by endogenous factors, the resistance might also originate from mutations induced by certain antibiotics, such as the fluoroquinolones. Such antibiotics increase the genome-wide mutation rate by introducing replication errors from the SOS response pathway or decreasing the efficiency of the DNA repair systems. However, the relative contributions of these molecular mechanisms remain unclear, hindering understanding of the generation of resistant pathogens. Here, using newly-accumulated mutations of wild-type and SOS-uninducible Escherichia coli strains, as well as those of the strains deficient for the mismatch repair (MMR) and the oxidative damage repair pathways, we find that the SOS response is the major mutagenesis contributor in mutation elevation, responsible for ~ 30–50% of the total base-pair substitution (BPS) mutation-rate elevation upon treatment with sublethal levels of norfloxacin (0 ~ 50 ng/mL). We further estimate the significance of the effects on other mutational features of these mechanisms (i.e., transversions, structural variations, and mutation spectrum) in E. coli using linear models. The SOS response plays a positive role in all three mutational features (mutation rates of BPSs, transversions, structural variations) and affects the mutational spectrum. The repair systems significantly reduce the BPS mutation rate and the transversion rate, regardless of whether antibiotics are present, while significantly increasing the structural variation rate in E. coli . Our results quantitatively disentangle the contributions of the SOS response and DNA repair systems in antibiotic-induced mutagenesis.","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

On four epibiotic peritrichous ciliates (Protozoa, Ciliophora) found in Lake Weishan Wetland: morphological and molecular data support the establishment of a new genus, Parapiosoma gen. nov., and two new species. 在微山湖湿地发现的四种表生性缘毛纤毛虫（原生动物、西里奥虫）：形态学和分子数据支持建立一个新属Parapiosoma gen.nov.和两个新种。

IF 5.7 2区生物学

Marine Life Science & Technology Pub Date : 2023-08-22 eCollection Date: 2023-08-01 DOI: 10.1007/s42995-023-00184-z

Tong Wu, Ting Cheng, Xiao Cao, Yaohan Jiang, Khaled A S Al-Rasheid, Alan Warren, Zhe Wang, Borong Lu

{"title":"On four epibiotic peritrichous ciliates (Protozoa, Ciliophora) found in Lake Weishan Wetland: morphological and molecular data support the establishment of a new genus, Parapiosoma gen. nov., and two new species.","authors":"Tong Wu, Ting Cheng, Xiao Cao, Yaohan Jiang, Khaled A S Al-Rasheid, Alan Warren, Zhe Wang, Borong Lu","doi":"10.1007/s42995-023-00184-z","DOIUrl":"10.1007/s42995-023-00184-z","url":null,"abstract":"During a study on the diversity of ciliated protists in Lake Weishan Wetland, the largest wetland in northern China, four epibiotic sessilid peritrichs were isolated from aquatic host animals. Two of them, i.e., Epistylis cambari Kellicott, 1885 and Epistylis lwoffi Fauré-Fremiet, 1943, were known species whereas the other two, i.e., Parapiosoma typicum gen. nov., sp. nov. and Orborhabdostyla gracilis sp. nov., are new to science. The new genus Parapiosoma gen. nov. is characterized by its branched non-contractile stalk, everted peristomial lip, obconical macronucleus and transverse silverlines. Two species are assigned to the new genus, namely Parapiosoma typicum sp. nov. and Parapiosoma gasterostei (Fauré-Fremiet, 1905) comb. nov. Morphologically, P. typicum sp. nov. is recognized by its goblet-shaped zooids, single-layered peristomial lip, dichotomously branched stalk, and infundibular polykinety 3 (P3) containing three equal-length rows. Orborhabdostyla gracilis sp. nov. is characterized by its slender zooid, curved macronucleus, and three equal-length rows in infundibular P3. Improved diagnoses and redescriptions of E. cambari and E. lwoffi are provided including, for the first time, data on the ciliature of E. cambari. Phylogenetic analyses based on SSU rDNA, ITS1-5.8S rDNA -ITS2, and LSU rDNA sequence data strongly support the assertion that the family Epistylididae comprises morphospecies with different evolutionary lineages and indicate that Parapiosoma gen. nov. may represent a new taxon at family level.","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 3","pages":"337-358"},"PeriodicalIF":5.7,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10164512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large yellow croaker (Larimichthys crocea) mitofusin 2 inhibits type I IFN responses by degrading MAVS via enhanced K48-linked ubiquitination. 大黄鱼线粒体融合蛋白2通过增强K48连接的泛素化降解MAVS来抑制I型IFN反应。

IF 5.8 2区生物学

Marine Life Science & Technology Pub Date : 2023-08-18 eCollection Date: 2023-08-01 DOI: 10.1007/s42995-023-00189-8

Wen-Xing Li, Xiao-Hong Wang, Yi-Jun Lin, Yuan-Yuan Zhou, Jun Li, Xiang-Yang Zhang, Xin-Hua Chen

{"title":"Large yellow croaker (Larimichthys crocea) mitofusin 2 inhibits type I IFN responses by degrading MAVS via enhanced K48-linked ubiquitination.","authors":"Wen-Xing Li, Xiao-Hong Wang, Yi-Jun Lin, Yuan-Yuan Zhou, Jun Li, Xiang-Yang Zhang, Xin-Hua Chen","doi":"10.1007/s42995-023-00189-8","DOIUrl":"10.1007/s42995-023-00189-8","url":null,"abstract":"In mammals, mitofusin 2 (MFN2) is involved in mitochondrial fusion, and suppresses the virus-induced RIG-I-like receptor (RLR) signaling pathway. However, little is known about the function of MFN2 in non-mammalian species. In the present study, we cloned an MFN2 ortholog (LcMFN2) in large yellow croaker (Larimichthys crocea). Phylogenetic analysis showed that MFN2 emerged after the divergence of amphioxus and vertebrates. The protein sequences of MFN2 were well conserved from fish to mammals. LcMFN2 was expressed in all the tissues/organs examined at different levels, and its expression was upregulated in response to poly(I:C) stimulation. Overexpression of LcMFN2 inhibited MAVS-induced type I interferon (IFN) promoter activation and antiviral gene expression. In contrast, knockdown of endogenous LcMFN2 enhanced poly(I:C) induced production of type I IFNs. Additionally, LcMFN2 enhanced K48-linked polyubiquitination of MAVS, promoting its degradation. Also, overexpression of LcMFN2 impaired the cellular antiviral response, as evidenced by the increased expression of viral genes and more severe cytopathic effects (CPE) in cells infected with spring viremia of carp virus (SVCV). These results indicated that LcMFN2 inhibited type I IFN response by degrading MAVS, suggesting its negative regulatory role in cellular antiviral response. Therefore, our study sheds a new light on the regulatory mechanisms of the cellular antiviral response in teleosts.Supplementary information: The online version contains supplementary material available at 10.1007/s42995-023-00189-8.","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 3","pages":"359-372"},"PeriodicalIF":5.8,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10110066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An improved blood hemorrhaging treatment using diatoms frustules, by alternating Ca and light levels in cultures. 一种改进的血液出血治疗方法，使用硅藻截头体，通过交替培养中的钙和光照水平。

IF 5.7 2区生物学

Marine Life Science & Technology Pub Date : 2023-08-18 eCollection Date: 2023-08-01 DOI: 10.1007/s42995-023-00180-3

Qinfeng Li, Zheng He, Hussein E Rozan, Chao Feng, Xiaojie Cheng, Xiguang Chen

{"title":"An improved blood hemorrhaging treatment using diatoms frustules, by alternating Ca and light levels in cultures.","authors":"Qinfeng Li, Zheng He, Hussein E Rozan, Chao Feng, Xiaojie Cheng, Xiguang Chen","doi":"10.1007/s42995-023-00180-3","DOIUrl":"10.1007/s42995-023-00180-3","url":null,"abstract":"Hemorrhage control requires hemostatic materials that are both effective and biocompatible. Among these, diatom biosilica (DBs) could significantly improve hemorrhage control, but it induces hemolysis (the hemolysis rate > 5%). Thus, the purpose of this study was to explore the influence of Ca2+ biomineralization on DBs for developing fast hemostatic materials with a low hemolysis rate. Here, CaCl2 was added to the diatom medium under high light (cool white, fluorescent lamps, 67.5 µmol m-2 s-1), producing Ca-DBs-3 with a particle size of 40-50 μm and a Ca2+ content of Ca-DBs-3 obtained from the higher concentration CaCl2 group (6.7 mmol L-1) of 0.16%. The liquid absorption capacity of Ca-DBs-3 was 30.43 ± 0.57 times its dry weight; the in vitro clotting time was comparable to QuikClot® zeolite; the hemostatic time and blood loss using the rat tail amputation model were 36.40 ± 2.52 s and 0.39 ± 0.12 g, which were 40.72% and 19.50% of QuikClot® zeolite, respectively. Ca-DBs-3 showed no apparent toxicity to L929 cells (cell viability > 80%) and was non-hemolysis (the hemolysis rate < 2%). This study prepared Ca-DBs-3 with a rapid hemostatic effect and good biocompatibility, providing a path to develop diatom biosilica hemostatic materials.Supplementary information: The online version contains supplementary material available at 10.1007/s42995-023-00180-3.","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 3","pages":"316-325"},"PeriodicalIF":5.7,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10110067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0