Marine Life Science & Technology最新文献

{"title":"Mating behaviors in ovoviviparous black rockfish (Sebastes schlegelii): molecular function of prostaglandin E2 as both a hormone and pheromone","authors":"Likang Lyu, Yijia Yao, Songyang Xie, Xiaojie Wang, Haishen Wen, Yun Li, Jianshuang Li, Chenpeng Zuo, Shaojing Yan, Jingyi Dong, Xin Qi","doi":"10.1007/s42995-023-00214-w","DOIUrl":"https://doi.org/10.1007/s42995-023-00214-w","url":null,"abstract":"","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139525100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional regulation analysis reveals the complexity of metamorphosis in the Pacific oyster (<i>Crassostrea gigas</i>).","authors":"Fei Xu, Shaoxi Deng, Daria Gavriouchkina, Guofan Zhang","doi":"10.1007/s42995-023-00204-y","DOIUrl":"10.1007/s42995-023-00204-y","url":null,"abstract":"<p><p>Many marine invertebrate phyla are characterized by indirect development. These animals transit from planktonic larvae to benthic spats via settlement and metamorphosis, which contributes to their adaption to the marine environment. Studying the biological process of metamorphosis is, thus, key to understanding the origin and evolution of indirect development. Although numerous studies have been conducted on the relationship between metamorphosis and the marine environment, microorganisms, and neurohormones, little is known about gene regulation network (GRN) dynamics during metamorphosis. Metamorphosis-competent pediveligers of the Pacific oyster <i>Crassostrea gigas</i> were assayed in this study. By assaying gene expression patterns and open chromatin region changes of different samples of larvae and spats, the dynamics of molecular regulation during metamorphosis were examined. The results indicated significantly different gene regulation networks before, during and post-metamorphosis. Genes encoding membrane-integrated receptors and those related to the remodeling of the nervous system were upregulated before the initiation of metamorphosis. Massive biogenesis, e.g., of various enzymes and structural proteins, occurred during metamorphosis as inferred from the comprehensive upregulation of the protein synthesis system post epinephrine stimulation. Hierarchical downstream gene networks were then stimulated. Some transcription factors, including homeobox, basic helix-loop-helix and nuclear receptors, showed different temporal response patterns, suggesting a complex GRN during the transition stage. Nuclear receptors, as well as their retinoid X receptor partner, may participate in the GRN controlling oyster metamorphosis, indicating an ancient role of the nuclear receptor regulation system in animal metamorphosis.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00204-y.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138479288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics and functions of E-cadherin complexes in epithelial cell and tissue morphogenesis.","authors":"Na Zhang, Matthias Häring, Fred Wolf, Jörg Großhans, Deqing Kong","doi":"10.1007/s42995-023-00206-w","DOIUrl":"10.1007/s42995-023-00206-w","url":null,"abstract":"<p><p>Cell-cell adhesion is at the center of structure and dynamics of epithelial tissue. E-cadherin-catenin complexes mediate Ca²⁺-dependent <i>trans-</i>homodimerization and constitute the kernel of adherens junctions. Beyond the basic function of cell-cell adhesion, recent progress sheds light the dynamics and interwind interactions of individual E-cadherin-catenin complex with E-cadherin superclusters, contractile actomyosin and mechanics of the cortex and adhesion. The nanoscale architecture of E-cadherin complexes together with <i>cis-</i>interactions and interactions with cortical actomyosin adjust to junctional tension and mechano-transduction by reinforcement or weakening of specific features of the interactions. Although post-translational modifications such as phosphorylation and glycosylation have been implicated, their role for specific aspects of in E-cadherin function has remained unclear. Here, we provide an overview of the E-cadherin complex in epithelial cell and tissue morphogenesis focusing on nanoscale architectures by super-resolution approaches and post-translational modifications from recent, in particular in vivo, studies. Furthermore, we review the computational modelling in E-cadherin complexes and highlight how computational modelling has contributed to a deeper understanding of the E-cadherin complexes.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138479281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invasion by exogenous RNA: cellular defense strategies and implications for RNA inference.","authors":"Danxu Tang, Yan Liu, Chundi Wang, Lifang Li, Saleh A Al-Farraj, Xiao Chen, Ying Yan","doi":"10.1007/s42995-023-00209-7","DOIUrl":"10.1007/s42995-023-00209-7","url":null,"abstract":"<p><p>Exogenous RNA poses a continuous threat to genome stability and integrity across various organisms. Accumulating evidence reveals complex mechanisms underlying the cellular response to exogenous RNA, including endo-lysosomal degradation, RNA-dependent repression and innate immune clearance. Across a variety of mechanisms, the natural anti-sense RNA-dependent defensive strategy has been utilized both as a powerful gene manipulation tool and gene therapy strategy named RNA-interference (RNAi). To optimize the efficiency of RNAi silencing, a comprehensive understanding of the whole life cycle of exogenous RNA, from cellular entry to its decay, is vital. In this paper, we review recent progress in comprehending the recognition and elimination of foreign RNA by cells, focusing on cellular entrance, intracellular transportation, and immune-inflammatory responses. By leveraging these insights, we highlight the potential implications of these insights for advancing RNA interference efficiency, underscore the need for future studies to elucidate the pathways and fates of various exogenous RNA forms, and provide foundational information for more efficient RNA delivery methods in both genetic manipulation and therapy in different organisms.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138479285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special topic on EvoDevo: emerging models and perspectives.","authors":"Hongan Long, Bo Dong","doi":"10.1007/s42995-023-00208-8","DOIUrl":"10.1007/s42995-023-00208-8","url":null,"abstract":"","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138479287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a developmental atlas and transgenetic tools in the ascidian <i>Styela clava</i>.","authors":"Boyan Lin, Wenjie Shi, Qiongxuan Lu, Takumi T Shito, Haiyan Yu, Bo Dong","doi":"10.1007/s42995-023-00200-2","DOIUrl":"10.1007/s42995-023-00200-2","url":null,"abstract":"<p><p>The ascidian <i>Styela clava</i> is an ecologically important species that is distributed along coastal regions worldwide. It has a long history as a model animal for evolutionary and developmental biology research owing to its phylogenetic position between vertebrates and invertebrates, and its classical mosaic expression patterns. However, the standard developmental atlas and protocols and tools for molecular manipulation of this organism are inadequate. In this study, we established a standard developmental table and provided a web-based digital image resource for <i>S. clava</i> embryogenesis at each developmental stage from fertilized eggs to hatching larvae by utilizing confocal laser microscopy and 3D reconstruction images. It takes around 10 h for fertilized eggs to develop into swimming larvae and 20-30 min to complete the tail regression processes at the metamorphic stage. We observed that the notochord cells in <i>S. clava</i> embryos did not produce an extracellular lumen like <i>Ciona robusta</i>, but showed polarized elongation behaviors, providing us an ideal comparative model to study tissue morphogenesis. In addition, we established a chemical-washing procedure to remove the chorion easily from the fertilized eggs. Based on the dechorionation technique, we further realized transgenic manipulation by electroporation and successfully applied tissue-specific fluorescent labeling in <i>S. clava</i> embryos. Our work provides a standard imaging atlas and powerful genetic tools for investigating embryogenesis and evolution using <i>S. clava</i> as a model organism.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00200-2.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138479282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary traits and functional roles of chemokines and their receptors in the male pregnancy of the Syngnathidae.","authors":"Han Jiang, Zhanwei Zhao, Haiyan Yu, Qiang Lin, Yali Liu","doi":"10.1007/s42995-023-00205-x","DOIUrl":"10.1007/s42995-023-00205-x","url":null,"abstract":"<p><p>Vertebrates have developed various modes of reproduction, some of which are found in Teleosts. Over 300 species of the Syngnathidae (seahorses, pipefishes and seadragons) exhibit male pregnancies; the males have specialized brood pouches that provide immune protection, nourishment, and oxygen regulation. Chemokines play a vital role at the mammalian maternal-fetal interface; however, their functions in fish reproduction are unclear. This study revealed the evolutionary traits and potential functions of chemokine genes in 22 oviparous, ovoviviparous, and viviparous fish species through comparative genomic analyses. Our results showed that chemokine gene copy numbers and evolutionary rates vary among species with different modes of reproduction. Syngnathidae lost <i>cxcl13</i> and <i>cxcr5</i>, which are involved in key receptor-ligand pairs for lymphoid organ development. Notably, Syngnathidae have site-specific mutations in <i>cxcl12b</i> and <i>ccl44</i>, suggesting immune function during gestation. Moreover, transcriptome analysis revealed that chemokine gene expression varies among Syngnathidae species with different types of brood pouches, suggesting adaptive variations in chemokine functions among seahorses and their relatives. Furthermore, challenge experiments on seahorse brood pouches revealed a joint immune function of chemokine genes during male pregnancy. This study provides insights into the evolutionary diversity of chemokine genes associated with different reproductive modes in fish.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00205-x.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138479283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene duplication and functional divergence of new genes contributed to the polar acclimation of Antarctic green algae.","authors":"Xiaowen Zhang, Wentao Han, Xiao Fan, Yitao Wang, Dong Xu, Ke Sun, Wei Wang, Yan Zhang, Jian Ma, Naihao Ye","doi":"10.1007/s42995-023-00203-z","DOIUrl":"10.1007/s42995-023-00203-z","url":null,"abstract":"<p><p>Psychrophilic microalgae successfully survive in the extreme and highly variable polar ecosystems, which represent the energy base of most food webs and play a fundamental role in nutrient cycling. The success of microalgae is rooted in their adaptive evolution. Revealing how they have evolved to thrive in extreme polar environments will help us better understand the origin of life in polar ecosystems. We isolated a psychrophilic unicellular green alga, <i>Microglena</i> sp. YARC, from Antarctic sea ice which has a huge genome. Therefore, we predicted that gene replication may play an important role in its polar adaptive evolution. We found that its protein-coding gene number significantly increased and the duplication time was dated between 37 and 48 million years ago, which is consistent with the formation of the circumpolar Southern Ocean. Most duplicated paralogous genes were enriched in pathways related to photosynthesis, DNA repair, and fatty acid metabolism. Moreover, there were a total of 657 <i>Microglena</i>-specific families, including collagen-like proteins. The divergence in the expression patterns of the duplicated and species-specific genes reflects sub- and neo-functionalization during stress acclimation. Overall, key findings from this study provide new information on how gene duplication and their functional novelty contributed to polar algae adaptation to the highly variable polar environmental conditions.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00203-z.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138479284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of the cardiac structure and transcriptome of scallop and snail, perspectives on heart chamber evolution.","authors":"Meina Lu, Rabia Hayat, Xuejiao Zhang, Yaqi Jiao, Jianyun Huang, Yifan Huangfu, Mingcan Jiang, Jieyi Fu, Qingqiu Jiang, Yaojia Gu, Shi Wang, Alexander A Akerberg, Ying Su, Long Zhao","doi":"10.1007/s42995-023-00202-0","DOIUrl":"10.1007/s42995-023-00202-0","url":null,"abstract":"<p><p>The evolution of a two-chambered heart, with an atrium and a ventricle, has improved heart function in both deuterostomes (vertebrates) and some protostomes (invertebrates). Although studies have examined the unique structure and function of these two chambers, molecular comparisons are few and limited to vertebrates. Here, we focus on the two-chambered protostome heart of the mollusks, offering data that may provide a better understanding of heart evolution. Specifically, we asked if the atrium and ventricle differ at the molecular level in the mollusk heart. To do so, we examined two very different species, the giant African land snail (<i>Lissachatina fulica</i>) and the relatively small, aquatic yesso scallop (<i>Mizuhopecten yessoensis</i>), with the assumption that if they exhibited commonality these similarities would likely reflect those across the phylum. We found that, although the hearts of these two species differed histologically, their cardiac gene function enrichments were similar, as revealed by transcriptomic analysis. Furthermore, the atrium and ventricle in each species had distinct gene function clusters, suggesting an evolutionary differentiation of cardiac chambers in mollusks. Finally, to explore the relationship between vertebrate and invertebrate two-chambered hearts, we compared our transcriptomic data with published data from the zebrafish, a well-studied vertebrate model with a two-chambered heart. Our analysis indicated a functional similarity of ventricular genes between the mollusks and the zebrafish, suggesting that the ventricle was differentiated to achieve the same functions in invertebrates and vertebrates. As the first such study on protostomes, our findings offered initial insights into how the two-chambered heart arose, including a possible understanding of its occurrence in both protostomes and deuterostomes.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00202-0.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138479280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenomic analyses shed light on the relationships of chiton superfamilies and shell-eye evolution.","authors":"Xu Liu, Julia D Sigwart, Jin Sun","doi":"10.1007/s42995-023-00207-9","DOIUrl":"10.1007/s42995-023-00207-9","url":null,"abstract":"<p><p>Mollusca is the second-largest animal phylum with over 100,000 extant species representing eight classes. Across 1000 extant species in the class Polyplacophora, chitons have a relatively constrained morphology but with some notable deviations. Several genera possess \"shell eyes\", i.e., true eyes with a lens and retina that are embedded within the dorsal shells. The phylogeny of the major chiton clades is mostly well established, in a set of superfamily-level and higher level taxa supported by various approaches, including morphological studies, multiple gene markers, mitogenome-phylogeny, and phylotranscriptomic approaches. However, one critical lineage has remained unclear, namely <i>Schizochiton</i> which was controversially suggested as being the potential independent origin of chiton shell eyes. Here, with the draft genome sequencing of <i>Schizochiton incisus</i> (superfamily Schizochitonoidea) plus assemblies of transcriptome data from other polyplacophorans, we present phylogenetic reconstructions using both mitochondrial genomes and phylogenomic approaches with multiple methods. We found that phylogenetic trees from mitogenomic data are inconsistent, reflecting larger scale confounding factors in molluscan mitogenomes. However, a consistent and robust topology was generated with protein-coding genes using different models and methods. Our results support Schizochitonoidea as the sister group to other Chitonoidea in Chitonina, in agreement with the established classification. Combined with evidence from fossils, our phylogenetic results suggest that the earliest origin of shell eyes is in Schizochitonoidea, and that these structures were also gained secondarily in other genera in Chitonoidea. Our results have generated a holistic review of the internal relationship within Polyplacophora, and a better understanding of the evolution of Polyplacophora.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138479286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}