Marine Life Science & Technology最新文献

{"title":"Chemical interactions between kelp <i>Macrocystis pyrifera</i> and symbiotic bacteria under elevated CO₂ condition.","authors":"Xiaowen Zhang, Tianle Xi, Yitao Wang, Xiao Fan, Dong Xu, Pengyan Zhang, Ke Sun, Yan Zhang, Jian Ma, Naihao Ye","doi":"10.1007/s42995-024-00259-5","DOIUrl":"https://doi.org/10.1007/s42995-024-00259-5","url":null,"abstract":"<p><p>Kelps are pivotal to temperate coastal ecosystems, providing essential habitat and nutrients for diverse marine life, and significantly enhancing local biodiversity. The impacts of elevated CO₂ levels on kelps may induce far-reaching effects throughout the marine food web, with potential consequences for biodiversity and ecosystem functions. This study considers the kelp <i>Macrocystis pyrifera</i> and its symbiotic microorganisms as a holistic functional unit (holobiont) to examine their collective response to heightened CO₂ levels. Over a 4 month cultivation from the fertilization of <i>M. pyrifera</i> gametes to the development of juvenile sporophytes, our findings reveal that elevated CO₂ levels influence the structure of the <i>M. pyrifera</i> symbiotic microbiome, alter metabolic profiles, and reshape microbe-metabolite interactions using 16S rRNA amplicon sequencing and liquid chromatography coupled to mass spectrometry analysis. Notably, <i>Dinoroseobacter</i>, <i>Sulfitobacter</i>, <i>Methylotenera</i>, <i>Hyphomonas</i>, Milano-WF1B-44 and <i>Methylophaga</i> were selected as microbiome biomarkers, which showed significant increases in comparative abundance with elevated CO₂ levels. Stress-response molecules including fatty-acid metabolites, oxylipins, and hormone-like compounds such as methyl jasmonate and prostaglandin F2a emerged as critical metabolomic indicators. We propose that elevated CO₂ puts certain stress on the <i>M. pyrifera</i> holobiont, prompting the release of these stress-response molecules. Moreover, these molecules may aid the kelp's adaptation by modulating the microbial community structure, particularly influencing potential pathogenic bacteria, to cope with environmental change. These results will enrich the baseline data related to the chemical interactions between the microbiota and <i>M. pyrifera</i> and provide clues for predicting the resilience of kelps to future climate change.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-024-00259-5.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"6 4","pages":"700-712"},"PeriodicalIF":5.8,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11602886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142774800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping the potential for global offshore finfish mariculture.","authors":"Shuang-En Yu, Ming-Ling Liao, Shuang-Lin Dong, Yun-Wei Dong","doi":"10.1007/s42995-024-00257-7","DOIUrl":"https://doi.org/10.1007/s42995-024-00257-7","url":null,"abstract":"<p><p>Understanding the potential areas suitable for offshore mariculture is crucial to global seafood security. Here, we map the potential global offshore mariculture areas for 23 principal commercial finfish using an ensemble model. The model involves the temporal-spatial heterogeneity of environments and constraints of temperature-dependent hypoxia and cold edges of cultured finfish by metabolic index and lower thermal safety margin, respectively. Our results show that currently, there is 9.16 ± 1.22 million km² of potentially suitable area for offshore finfish mariculture. Under climate change, the potential suitable area will be reduced to between 86.7% and 91.7% of the current size by 2050. Compared to the decline in tropical regions, the expanded potential areas in temperate and polar regions will become more important for global seafood security. The potential offshore finfish mariculture area responds differently to global change among species, and cold-water finfish may benefit from global warming. Overall, despite changes in the distribution of global offshore mariculture areas and replacements of local potential mariculture species, offshore finfish mariculture still holds immense potential in the future.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-024-00257-7.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"6 4","pages":"651-664"},"PeriodicalIF":5.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11602909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142774132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomy and systematic positions of three new <i>Callistoctopus</i> species (Octopoda, Octopodidae) discovered in coastal waters of China.","authors":"Yan Tang, Xiaodong Zheng, Chungcheng Lu","doi":"10.1007/s42995-024-00258-6","DOIUrl":"https://doi.org/10.1007/s42995-024-00258-6","url":null,"abstract":"<p><p>The genus <i>Callistoctopus</i> comprises 13 species, and has been reported mostly in the Western Pacific Ocean. Here, we described three new species from China, <i>Ca. paucilamellus</i> sp. nov., <i>Ca. sparsus</i> sp. nov., and <i>Ca. gracilis</i> sp. nov., based on morphometric and meristic characteristics. The diagnoses, descriptions and detailed morphometric data are provided for each species. The cytochrome c oxidase I (COI) genes of the three new species are sequenced, and compared with related species and analyzed for their systematic positions. Both phylogenetic trees constructed using three mitochondrial genes (12S rRNA, 16S rRNA, COI) and one nuclear gene (Rhodopsin) revealed that our new species formed into two distinct clades with strong support values. One clade included <i>Ca. gracilis</i> sp. nov., <i>Ca.</i> sp. 1, <i>Ca. xiaohongxu</i>, <i>Ca. tenuipes</i> and <i>Ca. paucilamellus</i> sp. nov., which clustered together. The other clade showed that <i>Ca. sparsus</i> sp. nov. was closely related to <i>Ca.</i> sp. 2 and <i>Ca.</i> sp. 3. <i>Ca. luteus</i> and <i>Ca. macropus</i> were located at the base of the <i>Callistoctopus</i> group. Based on our integrative studies, both morphological and molecular evidence suggested strongly that <i>O. minor</i> is more likely to be classified as a species of <i>Callistoctopus</i>. Morphological comparisons were made between the three new species and related taxa, which could be recognized based on the 7-8 gill lamellae of each demibranch, numerous small black spots on the subdermal layer of the arms, and an elongated body.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-024-00258-6.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"6 4","pages":"750-767"},"PeriodicalIF":5.8,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11602926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142774680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing an assessment tool for integrated evaluation of sustainable fisheries in China.","authors":"Qi Ding, Xiujuan Shan, Xianshi Jin, Harry Gorfine, Jiting Sun","doi":"10.1007/s42995-024-00251-z","DOIUrl":"https://doi.org/10.1007/s42995-024-00251-z","url":null,"abstract":"<p><p>Fisheries are social-ecological systems. Evaluating the sustainability of fisheries requires methods to measure performance from ecological, economic, social, and governance aspects. Whereas a number of multi-dimensional evaluation tools such as fishery performance indicators (FPIs) have been used for assessing fishery management systems, fishery management practices and data availability are likely to differ substantially among fisheries in different countries. This makes it at least somewhat problematic to precisely adapt this methodology to fisheries within a given country. This study constructed a practical tool to evaluate and compare fishery systems in China. On the basis of an established indicator library and the FPIs tool, indicators in the newly developed tool for comprehensive fisheries evaluation were selected according to the management objectives, data availability, and the authority of relevant indicators. The sustainability assessment tool for Chinese fisheries (SAT-fish) provides a three-tier hierarchical framework covering 60 indicators, of which 48 indicators were extracted from the FPIs tool and 12 indicators were associated with policy statements. Applicability and comprehensiveness of this tool in comparison with six other well-established frameworks were investigated. This tool offers a promising new method to assess the sustainability of fishery systems in China, with great potential to guide Chinese fisheries towards a higher level of sustainability.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-024-00251-z.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"6 4","pages":"713-721"},"PeriodicalIF":5.8,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11602921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142774803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep learning for genomic selection of aquatic animals.","authors":"Yangfan Wang, Ping Ni, Marc Sturrock, Qifan Zeng, Bo Wang, Zhenmin Bao, Jingjie Hu","doi":"10.1007/s42995-024-00252-y","DOIUrl":"https://doi.org/10.1007/s42995-024-00252-y","url":null,"abstract":"<p><p>Genomic selection (GS) applied to the breeding of aquatic animals has been of great interest in recent years due to its higher accuracy and faster genetic progress than pedigree-based methods. The genetic analysis of complex traits in GS does not escape the current excitement around artificial intelligence, including a renewed interest in deep learning (DL), such as deep neural networks (DNNs), convolutional neural networks (CNNs), and autoencoders. This article reviews the current status and potential of DL applications in phenotyping, genotyping and genomic estimated breeding value (GEBV) prediction of GS. It can be seen from this article that CNNs obtain phenotype data of aquatic animals efficiently, and without injury; DNNs as single nucleotide polymorphism (SNP) variant callers are critical to have shown higher accuracy in assessments of genotyping for the next-generation sequencing (NGS); autoencoder-based genotype imputation approaches are capable of highly accurate genotype imputation by encoding complex genotype relationships in easily portable inference models; sparse DNNs capture nonlinear relationships among genes to improve the accuracy of GEBV prediction for aquatic animals. Furthermore, future directions of DL in aquaculture are also discussed, which should expand the application to more aquaculture species. We believe that DL will be applied increasingly to molecular breeding of aquatic animals in the future.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-024-00252-y.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"6 4","pages":"631-650"},"PeriodicalIF":5.8,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11602929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142774801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the role of microbes in health and disease of farmed aquatic organisms.","authors":"Cristiane C Thompson, Wilson Wasielesky, Felipe Landuci, Michele S Lima, Leonardo Bacha, Luciane M Perazzolo, Cátia Lourenço-Marques, Florbela Soares, Pedro Pousão-Ferreira, Larry Hanson, Bruno Gomez-Gil, Mateus Thompson, Tooba Varasteh, Tatiana A Silva, Jean Swings, Xiao-Hua Zhang, Wanderley de Souza, Fabiano L Thompson","doi":"10.1007/s42995-024-00248-8","DOIUrl":"https://doi.org/10.1007/s42995-024-00248-8","url":null,"abstract":"<p><p>Aquaculture is critical to reduce protein deficiencies and supplement the world's demand for seafood. However, the culture environment predisposes farmed animals to infectious diseases. In particular, the high density of fish, crustacean, mollusk, sea cucumber or algal species allows for the rapid spread of infectious diseases resulting in devastating losses. Massive amounts of antibiotics have been used to sustain aquaculture production. This has led to the critical need to evaluate the impact of current control measures and optimize disease management schemes with an emphasis on global impact and sustainability. Furthermore, local and global changes have enhanced the pathogens' effects over aquaculture settings because increased temperature and pollution may trigger virulence genes and toxin production. Technological developments including biofloc technology, integrated multitrophic systems, recirculating aquaculture systems and probiotics have contributed to enhancing aquaculture sustainability and reducing the need for high loads of antibiotics and other chemicals. Furthermore, biotechnological tools (e.g., omics and cell biology) have shed light on cellular processes in the health and disease of reared organisms. Metagenomics is a reliable and relatively quick tool to identify microbial communities in aquaculture settings.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"6 4","pages":"579-609"},"PeriodicalIF":5.8,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11602928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142774686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-gene-based investigation on the molecular phylogeny of the hypotrichous family Strongylidiidae (Protista, Ciliophora), with notes on the ontogeny of a new genus and new species.","authors":"Wenya Song, Shijing Zhang, Yuqing Li, Honggang Ma, Qiyu Li, Xiaotian Luo, Khaled A S Al-Rasheid, Hunter N Hines, Xiaoteng Lu","doi":"10.1007/s42995-024-00243-z","DOIUrl":"10.1007/s42995-024-00243-z","url":null,"abstract":"<p><p>Ciliates in the subclass Hypotrichia have long been difficult to classify as they are one of the most polymorphic and highly differentiated groups, leading to their systematics remaining unresolved. Phylogenetic relationships within the hypotrich family Strongylidiidae have been ambiguous due to discordance between the morphological and genetic data. In this study, a new strongylidiid genus <i>Heterouroleptus</i> is established, mainly based on the novel mode of origin of the ventral cirral rows: left ventral cirral row (LVR) originates from frontal-ventral-transverse cirral anlagen (FVTA) III (anterior portion), IV (middle portion), and V (rear portion); right ventral cirral row comes from the entire FVTA VI. A new species, <i>Heterouroleptus weishanensis</i> gen. nov., sp. nov., is investigated along with the morphometric and molecular data from a population of <i>Strongylidium wuhanense</i>. Eight new sequences and nuclear gene markers (single-gene and multi-gene) are provided to analyze the phylogenetic relationships of strongylidiids, with the COI gene utilized to uncover further genetic information at species level and below. The results reveal that: (1) Strongylidiidae is monophyletic and has a close relationship with Dorsomarginalia; (2) <i>Heterouroleptus</i> gen. nov. forms a clade that is sister to all the other strongylidiids; (3) <i>Hemiamphisiella</i> Foissner, 1988 and <i>Pseudouroleptus</i> Hemberger, 1985 should not be synonyms, and both genera should be subdivided due to their variable morphological characteristics; (4) LVR originating from three anlagen is a plesiomorphy of Strongylidiidae. The discovery of the origin of the LVR not only contributes to the establishment of the genus <i>Heterouroleptus</i>, but also helps to improve the diagnosis of the family Strongylidiidae.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-024-00243-z.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"6 3","pages":"442-461"},"PeriodicalIF":5.8,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11358561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pichia pastoris</i> composition expressed aerolysin mutant of <i>Aeromonas veronii</i> as an oral vaccine evaluated in zebrafish (<i>Danio rerio</i>).","authors":"Yuan-Yuan Yao, Qing-Shuang Zhang, Shu-Bin Liu, Hong-Wei Yang, Xing-Yu Chen, Ya-Lin Yang, Chen-Chen Gao, Chao Ran, Tsegay Teame, Zhen Zhang, Zhi-Gang Zhou","doi":"10.1007/s42995-024-00239-9","DOIUrl":"10.1007/s42995-024-00239-9","url":null,"abstract":"<p><p>Vaccines are one of the most practical means to stop the spreading of <i>Aeromonas veronii</i> in aquaculture. In this study, virulence factor aerolysin mutant NTaer which has lost its hemolytic activity was used as a target antigen. <i>Pichia pastoris</i> constitutive secretory expression NTaer (GS115-NTaer) was used as a potential safe oral vaccine to evaluate its effectiveness on zebrafish immunity. The result shows that vaccination of GS115- NTaer for four weeks did not affect the growth performance of the host, while eliciting an effective immune protective response. Compared with the control group, the GS115-NTaer could significantly up-regulate the relative expression level of the intestinal tight junction protein 1α (<i>TJP1α</i>) gene, and significantly increased the contents of lysozyme (LYZ), complement C3 and C4 in the gut, indicating that the innate immune response of the fish was activated. The relative gene expression levels of macrophage-expressed gene 1 (<i>MPEG1</i>) and T cell receptor (<i>TCR-α</i>) in the gut, and <i>MPEG1</i>, <i>CD4</i>, <i>CD8</i>, <i>TCR-α</i>, <i>GATA3,</i> and <i>T-bet</i> in the spleen were all increased significantly, indicating that the cellular immune response of the fish was activated. Furthermore, the contents of serum IgM and intestinal mucosa IgZ antibodies were significantly increased, which showed that humoral immunity was also activated. Moreover, inoculation with GS115-NTaer significantly changed the structure of gut microbiota. In particular, the relative ratio of (Firmicutes + Fusobacteriota + Bacteroidota)/Proteobacteria was significantly higher than that of the control and GS115 groups. Lastly, the vaccinated fish were challenged with <i>A. veronii,</i> and the relative percent survival of GS115 and the GS115-NTear groups was 14.28% and 33.43%. This improvement of immunity was not only due to the specific immune response but also attributed to the improvement of innate immunity and the gut microbiota which was demonstrated by the germ-free zebrafish model. Collectively, this study provides information on the effectiveness of GS115-NTear as an oral vaccine for the green prevention and control of <i>A. veronii</i> infection in fish aquaculture.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"6 3","pages":"475-487"},"PeriodicalIF":5.8,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11358560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of 12 water lily plastid genomes reveals genomic divergence and evolutionary relationships in early flowering plants.","authors":"Weicai Song, Wenbo Shi, Huan Wang, Zirui Zhang, Ruiqing Tao, Jin Liu, Shuo Wang, Michael S Engel, Chao Shi","doi":"10.1007/s42995-024-00242-0","DOIUrl":"10.1007/s42995-024-00242-0","url":null,"abstract":"<p><p>The aquatic plant <i>Nymphaea</i>, a model genus of the early flowering plant lineage Nymphaeales and family Nymphaeaceae, has been extensively studied. However, the availability of chloroplast genome data for this genus is incomplete, and phylogenetic relationships within the order Nymphaeales remain controversial. In this study, 12 chloroplast genomes of <i>Nymphaea</i> were assembled and analyzed for the first time. These genomes were 158,290-160,042 bp in size and contained 113 non-repeat genes, including 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. We also report on codon usage, RNA editing sites, microsatellite structures, and new repetitive sequences in this genus. Comparative genomics revealed that expansion and contraction of IR regions can lead to changes in the gene numbers. Additionally, it was observed that the highly variable regions of the chloroplast genome were mainly located in intergenic regions. Furthermore, the phylogenetic tree showed the order Nymphaeales was divided into three families, and the genus <i>Nymphaea</i> can be divided into five (or three) subgenera, with the subgenus <i>Nymphaea</i> being the oldest. The divergence times of nymphaealean taxa were analyzed, with origins of the order Nymphaeales and family Nymphaeaceae being about 194 and 131 million years, respectively. The results of the phylogenetic analysis and estimated divergence times will be useful for future evolutionary studies of basal angiosperm lineages.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-024-00242-0.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"6 3","pages":"425-441"},"PeriodicalIF":5.8,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11358372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of a potential bladder cancer inhibitor CHNQD-01281 by regulating EGFR and promoting infiltration of cytotoxic T cells.","authors":"Jian-Yu Liu, Yao-Yao Jiang, Peng-Jie Li, Bo Yao, Yi-Jing Song, Ji-Xiu Gao, Gulab Said, Yang Gao, Jun-Yu Lai, Chang-Lun Shao","doi":"10.1007/s42995-024-00246-w","DOIUrl":"10.1007/s42995-024-00246-w","url":null,"abstract":"<p><p>As one of the common malignancies that threaten human life, bladder cancer occurs frequently with a high mortality rate in the world, due to its invasion, recurrence and drug resistance. Natural products from marine microorganisms are becoming the hotspots in discovery of new candidate drug entities, especially in the area of cancer. Brefeldin A (BFA) is a natural Arf-GEFs inhibitor, but due to the low aqueous solubility, strong toxicity, and poor bioavailability, it is urgent to conduct structural optimization research. Herein, a new BFA pyridine acrylate derivative <b>CHNQD-01281</b> with improved solubility was prepared and found to exert moderate to strong antiproliferative activity on a variety of human cancer cell lines. It was noteworthy that <b>CHNQD-01281</b> was most sensitive to two bladder cancer cell lines T24 and J82 (IC₅₀ = 0.079 and 0.081 μmol/L) with high selectivity index (SI = 14.68 and 14.32), suggesting a superior safety to BFA. In vivo studies revealed that <b>CHNQD-01281</b> remarkably suppressed tumor growth in a T24 nude mice xenograft model (TGI = 52.63%) and prolonged the survival time (ILS = 68.16%) in an MB49 allogeneic mouse model via inducing infiltration of cytotoxic T cells. Further mechanism exploration indicated that <b>CHNQD-01281</b> regulated both EGFR/PI3K/AKT and EGFR/ERK pathways and mediated the chemotactic effect of chemokines on immune effector cells. Overall, <b>CHNQD-01281</b> may serve as a potential therapeutic agent for bladder cancer through multiple mechanisms.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-024-00246-w.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"6 3","pages":"502-514"},"PeriodicalIF":5.8,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11358582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}