Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology最新文献

{"title":"Mini Review; Role of Changes in SARS-CoV-2 Spike Protein and Its Human Interaction","authors":"Tahani N. Altamimi, F. Balouch","doi":"10.21608/eajbsc.2023.303781","DOIUrl":"https://doi.org/10.21608/eajbsc.2023.303781","url":null,"abstract":"","PeriodicalId":52600,"journal":{"name":"Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89226128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Evaluation of Long Non-Coding RNA (TUG1) in Systemic Lupus Erythematosus","authors":"S. Ramadan, W. Shousha, O. Shaker, N. Ahmed, S. Abdo","doi":"10.21608/eajbsc.2023.303332","DOIUrl":"https://doi.org/10.21608/eajbsc.2023.303332","url":null,"abstract":"Long non-coding RNAs play an important role in systemic lupus erythematosus. Our work purpose is to evaluate the level of LNC (TUG1) in SLE patients. This research includes 20 control and 70 SLE subjects. The serum level of LNC (TUG1) was measured by polymerase chain reaction. Down-expression of LNC (TUG1) was observed in SLE patients more than in control. A significant AUC of TUG1 level was recorded, which recommended that (TUG1) can act as a new biomarker for SLE determination .","PeriodicalId":52600,"journal":{"name":"Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87993166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymorphism in Long Non-Coding RNA- HOXA Transcript at The Distal Tip Among Rheumatoid Arthritis Patients","authors":"S. Abdo, W. Shousha, O. Shaker, A. Ali, Shaimaa M. Ramadan","doi":"10.21608/eajbsc.2023.302438","DOIUrl":"https://doi.org/10.21608/eajbsc.2023.302438","url":null,"abstract":"Limited studies have explored the correlation between lncRNA HOTTIP single nucleotide polymorphisms (SNPs) and RA. This study aimed to evaluate the genetic association of single nucleotide polymorphism rs3807598 (C:G) of HOTTIP among rheumatoid arthritis patients. Method: The study included 120 subjects from 2 groups: 60 RA patients and 60 healthy controls. Real-time PCR with TaqMan allelic discrimination assay was used to perform the genotyping. The odds ratios (ORs) models and 95% confidence interval (CIs) were used to test the associations. Results: The results indicated that lncRNA HOTTIP SNP rs3807598 (C:G) exhibited a statistically nonsignificant association with the risk of RA. Conclusion : Up-to-date, the role of HOTTIP SNPs in RA risk is still unknown, It is important to consider how epigenetic modifications including non-coding RNAs polymorphism and expression affect the RA risk and how well it responds to treatment. Further studies, with a larger sample size, could highlight more significant relations between SNPs of non-coding RNAs and RA.","PeriodicalId":52600,"journal":{"name":"Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88960484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Incidence and Molecular Characterization of Banana Bunchy Top Virus (BBTV) Infecting Banana (Musa spp.) in Ismailia governorate, Egypt","authors":"H. Amin, Azza G. Farag, Abeer Abouelwafa","doi":"10.21608/eajbsc.2023.301072","DOIUrl":"https://doi.org/10.21608/eajbsc.2023.301072","url":null,"abstract":"The banana is the most important fruit in Egypt. Banana bunchy top disease, caused by the banana bunchy top virus (BBTV), is one of the most damaging infections on banana plantations, resulting in significant crop losses. The current study was conducted to characterize the incidence of banana bunchy top disease (BBTD) and to study the molecular characteristics of BBTV from banana plantations in Ismailia. This study was conducted during the period 2019-2021 in three locations (Abu-Suwayr, El Tal-El kabir and Fayed) in Ismailia Governorate. The occurrence of BBTV was initially studied using visual inspection of external virus symptoms. TAS-ELISA, PCR and nucleic acid hybridization were used to detect the virus. Further molecular characterization of BBTV was performed based on Rolling Circle Amplification (RCA) and nucleotide sequencing. BBTD incidences ranging from 3.33 to 5.14% were observed in two localities Abu-Suwayr and El Tal-El kabir. While a relatively low incidence of 1.48% was observed in Fayed. Our results showed that the BBTD incidence is directly related to the percentage of winged aphids inside the aphid colonies regardless the percentage of the aphid's occurrence. The PCR product (973 bp) of BBTV-component 3 (DNA-S) was used for the creation of a non-radioactive Dig labeled-DNA probe for further use for BBTV detection using dot blot and Southern blot hybridization. The sequence comparison analysis of DNA-S component showed that the present Ismailia isolate (BBTV-3F) shared nucleotide identity 98.4%- 91.9 % with three isolates from Qalubya Egypt and shared 97.9% nt identity with east African isolates (Rwanda and Malawi). The phylogenetic analysis revealed that the DNA-component 3 (DNA-S) of the BBTV-3F isolate from Ismailia belongs to the South Pacific group (SPG) and is markedly separated from those of the Asian group (AG). This study is important for monitoring and early detection of BBTV threats and for rapid eradication of infected plants before the virus spreads and settles in a new area.","PeriodicalId":52600,"journal":{"name":"Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology","volume":"87 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89871800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic Detection of Metallo-Β Lactamase Producing Carbapenem-Resistant Pseudomonas Aeruginosa","authors":"S. Hariri, Suleman Khan","doi":"10.21608/eajbsc.2023.301238","DOIUrl":"https://doi.org/10.21608/eajbsc.2023.301238","url":null,"abstract":"","PeriodicalId":52600,"journal":{"name":"Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88163817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Biofilm Formation in Salmonella typhi Isolated from Enteritis Patients in Al-Najaf-Iraq","authors":"Nisreen Mahdi, Z. Motaweq","doi":"10.21608/eajbsc.2023.301062","DOIUrl":"https://doi.org/10.21608/eajbsc.2023.301062","url":null,"abstract":"Several types of bacteria enhance their survival by attaching to non-living surfaces or tissues, presenting them as multicellular communities covered by a protective extracellular matrix called biofilm. There has been a clear interest in assessing the relationship between antibiotic resistance phenotype and biofilm production. The aim of this paper was to present additional experimental results on this topic and to test the ability of Salmonella typhi isolates to biofilm formation using in vitro methods in the context of their antibiotic resistance. In the result of the study, 28 isolates of S. typhi were included. All 28 clinical specimens 100% showed strong biofilm formation and all clinical specimens showed the presence of pml gene by clear bands in gel electrophoresis. The results of the antibiotic sensitivity test of S. typhi isolates by disk diffusion method against 18 types of commonly used antibiotics showed a large variation in their rates of antibiotic resistance, as the highest percentage of resistance to the antibiotic to S . typhi resistance to the most common antibiotics used in treatment. The highest rate of resistance was seen with","PeriodicalId":52600,"journal":{"name":"Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87202606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect Of Cigarette Smoking on Nerve Conduction Velocity","authors":"R. Jeevalakshmi, R. Shanmughavadivu, S. Kavitha","doi":"10.21608/eajbsc.2023.300883","DOIUrl":"https://doi.org/10.21608/eajbsc.2023.300883","url":null,"abstract":"Background : Smoking causes changes in the myelin sheath leading to demyelination, causing blockage of nerve conduction and a decrease in conduction velocity. Aim: To assess the effect of cigarette smoking on nerve conduction velocity of median and ulnar nerve by nerve conduction study. Methodology: 50 smokers and 50 non-smokers were recruited and nerve conduction velocity of the median and ulnar nerve were studied in both groups. Result: The sensory nerve conduction velocity among smokers was significantly reduced when compared to non-smokers (p<0.05). Discussion: The chemical oxidants that are released from cigarette smoke cause neural ischemia and also enhance oxidative stress. The sensory nerve is a thin nerve and it is more prone to damage by the chemicals released from cigarette smoke. Conclusion: The sensory nerve conduction velocity among smokers was reduced in our study.","PeriodicalId":52600,"journal":{"name":"Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79827386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review on: Ashwagandha Root Extract Phenolic Compounds Counteract Alloxan's Effects on Oxidative Stress, Inflammation, and Peripheral Nerve Injury","authors":"Heba R. Hashem, Z. Nabil, H. G. Gad El-Hak","doi":"10.21608/eajbsc.2023.300377","DOIUrl":"https://doi.org/10.21608/eajbsc.2023.300377","url":null,"abstract":"","PeriodicalId":52600,"journal":{"name":"Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77567589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Serum Lipocalin-2 Level as Early Predictor Marker to Severe COVID-19 Disease","authors":"Shakir Abbas, H. Ali, R. Ali, M. Mashkur, Mohammed Hasan, A. Awad, Mohauman AL- Rufaie","doi":"10.21608/eajbsc.2023.299538","DOIUrl":"https://doi.org/10.21608/eajbsc.2023.299538","url":null,"abstract":"The coronavirus disease of 2019 (COVID-19) is an emerging coronavirus that affects people's respiratory systems (Severe acute respiratory syndrome coronavirus) and can the rapidly growing COVID-19 pandemic represents a significant global challenge, It can be considered that lipocalin-2 was highly associated with the severity of COVID-19. Therefore, it may be a useful biomarker for diagnosing disease severity in COVID-19 patients. lipocalin-2 was initially identified as a secreted protein from human neutrophils. Alveolar type II cells that have been damaged primarily express this substance. To verify lipocalin-2's potential as a diagnosing biomarker for COVID-19 patients, Lipocalin-2 levels in the blood were examined in this pilot investigation. To examine the relationship between serum lipocalin-2 levels and the severity of COVID-19 infection to see if this protein may be utilized as a disease indicator. This study was done in a case-control study with One hundred and twenty patients (79 males, 41 females) with COVID-19 who participated in the research. The COVID-19 patients were divided into three groups based on the severity of the illness: critical disease (n = 30), severe disease (n = 30), and mild/moderate disease (n = 60), with (n = 60) healthy volunteers serving as the control group (35 males, 25 females). Between January 2022 and May 2022, the patients were obtained from Al-Amal hospitals and the AL-Shefaa centre in ALNajaf City, Iraq., All of the patients' fundamental clinical and demographic data were collected along with blood samples. Enzyme-linked immunosorbent tests were used to measure the blood's level of LCN2 (ELISA). The levels of total cholesterol, triglycerides, and High-Density lipoprotein were assessed using colorimetric methods. Ichroma was tested for serum ferritin, D-dimer, and CBC by Swelab. ran a statistical study to see if they were related to the severity of the disease. Higher lipocalin-2 levels were observed in the patient group, particularly in cases of mild/moderate (1.32±0.30) (P. 0.001), severe(2.16±0.42) (P. 0.001), and critical(4.71±1.01) (P. 0.001) comparing cases to healthy controls (0.86±0.51) respectively, groupings. (SPO2 %, Hb, TC, HDL, LDL, and lymphocyte) levels were found to significant negative correlation with one another in the COVID-19 patient group, with p-values=0.001 for each of these relationships. Moreover, a significant positive correlation between (TG, VLDL-C, WBCs, neutrophil, platelet, N/L ratio, D-dimer, Ferritin, and CRP, p.value=0.001 for each one of them) levels with lipocalin-2 in the COVID-19 patients group. a cut-off value of 1.215 (ng/mL) for lipocalin-2 predicted severe COVID-19 with a sensitivity of 81.7 % and a specificity of 80.2 % (AUC: 0.9, 95%CI 0.852-0.949;p<0.0001). [ FROM AUTHOR] Copyright of Egyptian Academic Journal of Biological Sciences, C Physiology & Molecular Biology is the property of Egyptian Academic Journal of Biological Sciences and its content may not be cop","PeriodicalId":52600,"journal":{"name":"Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74750366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of the Drug Favipiravir in the Destruction of Spike Protein in Patients with Covid-19","authors":"A. Atiyah, Faesal Hassen","doi":"10.21608/eajbsc.2023.298541","DOIUrl":"https://doi.org/10.21608/eajbsc.2023.298541","url":null,"abstract":"The Spike protein is one of the virulence factors of viruses, which is responsible for the attachment of the virus to the host cell. This study included the study of the effect of Favipiravir on S protein at different concentrations, as it showed the highest effect at a concentration of 0.5 and an amount of 9.66 ± followed by the concentration. .025 by 7.06 ± to have a gradual effect of (5.23 ±, 3.63 ± 2.53 ±, 1.60 ±, 0.06 ±, 0.00) at a concentration of (0.015, 0.03, 0.06, 0.125, 0.07, 0.02), respectively, as once the virus interacts with the cell The host causes a structural rearrangement of the S protein, which allows the virus to fuse with the host cell membrane. Because of the seriousness of the Covid-19 virus and its components, it has become necessary to find a drug that controls the spread of the pandemic. [ FROM AUTHOR] Copyright of Egyptian Academic Journal of Biological Sciences, C Physiology & Molecular Biology is the property of Egyptian Academic Journal of Biological Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)","PeriodicalId":52600,"journal":{"name":"Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology","volume":"222 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75629561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}