Water Research X最新文献

{"title":"Ensuring microbial water quality for on-site water reuse: Importance of online sensors for reliable operation","authors":"Eva Reynaert ,&nbsp;Deepthi Nagappa ,&nbsp;Jürg A. Sigrist ,&nbsp;Eberhard Morgenroth","doi":"10.1016/j.wroa.2024.100215","DOIUrl":"10.1016/j.wroa.2024.100215","url":null,"abstract":"<div><p>A growing number of cities and regions are promoting or mandating on-site treatment and reuse of wastewater, which has resulted in the implementation of several thousand on-site water reuse systems on a global scale. However, there is only limited information on the (microbial) water quality from implemented systems. The focus of this study was on two best-in-class on-site water reuse systems in Bengaluru, India, which typically met the local water quality requirements during monthly compliance testing. This study aimed to (i) assess the microbial quality of the reclaimed water at a high temporal resolution (daily or every 15 min), and (ii) explore whether measurements from commercially available sensors can be used to improve the operation of such systems. The monitoring campaign revealed high variations in microbial water quality, even in these best-in-class systems, rendering the water inadequate for the intended reuse applications (toilet flushing and landscape irrigation). These variations were attributed to two key factors: (1) the low frequency of chlorination, and (2) fluctuations of the chlorine demand of the water, in particular of ammonium concentrations. Such fluctuations are likely inherent to on-site systems, which rely on a low level of process control. The monitoring campaign showed that the microbial water quality was most closely related to oxidation–reduction potential (ORP) and free chlorine sensors. Due to its relatively low cost and low need for maintenance, the ORP emerges as a compelling candidate for automating the chlorination to effectively manage variations in chlorine demand and ensure safe water reuse. Overall, this study underscores the necessity of integrating treatment trains, operation, and monitoring for safe on-site water reuse.</p></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":null,"pages":null},"PeriodicalIF":7.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589914724000057/pdfft?md5=1669853b85807b6e450d744ba8902ca0&pid=1-s2.0-S2589914724000057-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139966326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-cost monitoring systems for urban water management: Lessons from the field","authors":"Perrine Hamel ,&nbsp;Ning Ding ,&nbsp;Frederic Cherqui ,&nbsp;Qingchuan Zhu ,&nbsp;Nicolas Walcker ,&nbsp;Jean-Luc Bertrand-Krajewski ,&nbsp;Paskorn Champrasert ,&nbsp;Tim D. Fletcher ,&nbsp;David T. McCarthy ,&nbsp;Oldrich Navratil ,&nbsp;Baiqian Shi","doi":"10.1016/j.wroa.2024.100212","DOIUrl":"10.1016/j.wroa.2024.100212","url":null,"abstract":"<div><p>Sound urban water management relies on extensive and reliable monitoring of water infrastructure. As low-cost sensors and networks have become increasingly available for environmental monitoring, urban water researchers and practitioners must consider the benefits and disadvantages of such technologies. In this perspective paper, we highlight six technical and socio-technological considerations for low-cost monitoring technology to reach its full potential in the field of urban water management, including: technical barriers to implementation, complementarity with traditional sensing technologies, low-cost sensor reliability, added value of produced information, opportunities to democratize data collection, and economic and environmental costs of the technology. For each consideration, we present recent experiences from our own work and broader literature and identify future research needs to address current challenges. Our experience supports the strong potential of low-cost monitoring technology, in particular that it promotes extensive and innovative monitoring of urban water infrastructure. Future efforts should focus on more systematic documenting of experiences to lower barriers to designing, implementing, and testing of low-cost sensor networks, and on assessing the economic, social, and environmental costs and benefits of low-cost sensor deployments.</p></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":null,"pages":null},"PeriodicalIF":7.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589914724000021/pdfft?md5=5ec1fc74ede0d17a8625fa1460d119d4&pid=1-s2.0-S2589914724000021-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139631726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling sorption of environmental organic chemicals from water to soils","authors":"Zhizhen Zhang ,&nbsp;Shenghong Wang ,&nbsp;Trevor N. Brown ,&nbsp;Alessandro Sangion ,&nbsp;Jon A. Arnot ,&nbsp;Li Li","doi":"10.1016/j.wroa.2024.100219","DOIUrl":"https://doi.org/10.1016/j.wroa.2024.100219","url":null,"abstract":"<div><p>Reliable estimation of chemical sorption from water to solid phases is an essential prerequisite for reasonable assessments of chemical hazards and risks. However, current fate and exposure models mostly rely on algorithms that lack the capability to quantify chemical sorption resulting from interactions with multiple soil constituents, including amorphous organic matter, carbonaceous organic matter, and mineral matter. Here, we introduce a novel, generic approach that explicitly combines the gravimetric composition of various solid constituents and poly-parameter linear free energy relationships to calculate the solid-water sorption coefficient (<em>K</em>_d) for non-ionizable or predominantly neutral organic chemicals with diverse properties in a neutral environment. Our approach demonstrates an overall statistical uncertainty of approximately 0.9 log units associated with predictions for different types of soil. By applying this approach to estimate the sorption of 70 diverse chemicals from water to two types of soils, we uncover that different chemicals predominantly exhibit sorption onto different soil constituents. Moreover, we provide mechanistic insights into the limitation of relying solely on organic carbon normalized sorption coefficient (<em>K</em>_OC) in chemical hazard assessment, as the measured <em>K</em>_OC can vary significantly across different soil types, and therefore, a universal cut-off threshold may not be appropriate. This research highlights the importance of considering chemical properties and multiple solid constituents in sorption modeling and offers a valuable theoretical approach for improved chemical hazard and exposure assessments.</p></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":null,"pages":null},"PeriodicalIF":7.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589914724000094/pdfft?md5=d32ca406f1a7aef137a78002b436141e&pid=1-s2.0-S2589914724000094-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140341967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient utilization of photoelectron-hole at semiconductor-microbe interface for pyridine degradation with assistance of external electric field","authors":"Hefei Shi ,&nbsp;Wenbo Fan ,&nbsp;Xinbai Jiang ,&nbsp;Dan Chen ,&nbsp;Cheng Hou ,&nbsp;Yixuan Wang ,&nbsp;Yang Mu ,&nbsp;Jinyou Shen","doi":"10.1016/j.wroa.2024.100214","DOIUrl":"https://doi.org/10.1016/j.wroa.2024.100214","url":null,"abstract":"<div><p>In this study, enhanced pyridine bio-photodegradation with assistance of electricity was achieved. Meanwhile, photoelectron-hole played a vital role in accelerating pyridine biomineralization. The significant separation of photoelectron-hole was achieved with an external electric field, which provided sufficient electron donors and acceptors for pyridine biodegradation. The enhanced electron transport system activity also revealed the full utilization of photoelectron-hole by microbes at semiconductor-microbe interface with assistance of electricity. Microbial community analysis confirmed the enrichment of functional species related to pyridine biodegradation and electron transfer. Microbial function analysis and microbial co-occurrence networks analysis indicated that upregulated functional genes and positive interactions of different species were the important reasons for enhanced pyridine bio-photodegradation with external electric field. A possible mechanism of enhanced pyridine biodegradation was proposed, i.e., more photoelectrons and holes of semiconductors were utilized by microbes to accelerate reduction and oxidation of pyridine with the assistance of electrical stimulation. The excellent performance of the photoelectrical biodegradation system showed a potential alternative for recalcitrant organic wastewater treatment.</p></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":null,"pages":null},"PeriodicalIF":7.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589914724000045/pdfft?md5=65ee6fe5feed72805286bf20b4893cea&pid=1-s2.0-S2589914724000045-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139986152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research stories along the urban water cycle","authors":"Treavor H. Boyer ,&nbsp;Wolfgang Gernjak","doi":"10.1016/j.wroa.2024.100218","DOIUrl":"https://doi.org/10.1016/j.wroa.2024.100218","url":null,"abstract":"","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":null,"pages":null},"PeriodicalIF":7.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589914724000082/pdfft?md5=67a674643208679bfbb7471bb9c671a9&pid=1-s2.0-S2589914724000082-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140135131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Removal of urea in ultrapure water system by urease-coated reverse osmosis membrane","authors":"Seung-Ju Choi ,&nbsp;Lucas Crane ,&nbsp;Seoktae Kang ,&nbsp;Treavor H. Boyer ,&nbsp;François Perreault","doi":"10.1016/j.wroa.2024.100211","DOIUrl":"https://doi.org/10.1016/j.wroa.2024.100211","url":null,"abstract":"<div><p>Among the various substances found in the feed source for the production of ultrapure water (UPW), urea is challenging to remove because it is a small molecular weight molecule that is not easily oxidized and does not carry a charge under neutral pH conditions. Urease enzyme, found in various organisms such as plants and bacteria, catalyze the hydrolysis of urea into carbon dioxide and ammonia. In this study, urease was immobilized on the polyamide layer of a reverse osmosis (RO) membrane to remove urea in UPW systems. The removal efficiency of urea by urease-coated RO membrane showed up to 27.9 % higher urea removal efficiency compared to the pristine membrane. This increase in urea removal can be attributed to both physical and biological effects from the urease coating on the membrane. Firstly, urease on the membrane surface can act as an additional physical barrier for urea to pass through. Secondly, urea can be hydrolyzed by the enzyme when it passes through the urease-coated RO membrane. In a two-pass RO system typical for UPW production, the removal of urea by a urease-coated membrane would be enhanced by twofold. This overall method can significantly increase the removal efficiency of urea in UPW systems, especially when considering the compounded removal by the urease coating, rejection by RO, and additional reactions by other treatment processes. Moreover, urea in UPW systems can be removed without the installment of additional processes by simply coating urease on the existing RO membranes.</p></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":null,"pages":null},"PeriodicalIF":7.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S258991472400001X/pdfft?md5=7a947ef7253f7558d3798de9aa059fdb&pid=1-s2.0-S258991472400001X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139487597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient smartphone-based measurement of phosphorus in water","authors":"Haiping Ai,&nbsp;Kai Zhang,&nbsp;Huichun Zhang","doi":"10.1016/j.wroa.2024.100217","DOIUrl":"https://doi.org/10.1016/j.wroa.2024.100217","url":null,"abstract":"<div><p>Agricultural runoff is one of the main sources of excess phosphorus (P) in different water bodies, subsequently leading to eutrophication and harmful algal blooms. To effectively monitor P levels in water, there is a need for simple measurement tools and extensive public involvement to enable regular and widespread sampling. Several smartphone-based P measurement methods have been reported, which extract red-green-blue (RGB) values from colorimetric reactions to build statistical regression models for P quantification. However, these methods typically require meticulous light conditions, involve initial equipment investment, and have undergone limited testing for large-scale applications. To overcome these limitations, this study developed a smartphone-based, equipment-free and facile P colorimetric analysis method. Following the standard procedure of the ascorbic acid approach, colorimetric reactions were captured by a smartphone camera, and RGB values were extracted using Python code for modeling. Different indoor light conditions, phone types, containers, and types of water samples were examined, resulting in a collection of 1922 images. The best regression model, employing random forest with RGB values and container types as inputs, achieved an R² of 0.97 and an RMSE of 0.051 for P concentrations ranging from 0.01 to 1.0 mg P/L. Additionally, the optimal classification model could estimate the level of P below 0.1 mg P/L with an accuracy of 95.2 (or 77.4 % for &lt;0.05 mg P/L). The strong performance of the developed models, which are also available freely online, suggests an easy and effective tool for more frequent P measurement and greater public involvement.</p></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":null,"pages":null},"PeriodicalIF":7.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589914724000070/pdfft?md5=1ca9f055bd1a9fcb920a1fd605e425cd&pid=1-s2.0-S2589914724000070-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140069286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review of influenza virus in water environments across human, poultry, and wild bird habitats","authors":"S Kenmoe ,&nbsp;GR Takuissu ,&nbsp;JT Ebogo-Belobo ,&nbsp;C Kengne-Ndé ,&nbsp;DS Mbaga ,&nbsp;A Bowo-Ngandji ,&nbsp;JL Ondigui Ndzie ,&nbsp;R Kenfack-Momo ,&nbsp;S Tchatchouang ,&nbsp;R Lontuo Fogang ,&nbsp;E Zeuko'o Menkem ,&nbsp;GI Kame-Ngasse ,&nbsp;JN Magoudjou-Pekam ,&nbsp;S Puzelli ,&nbsp;L Lucentini ,&nbsp;C Veneri ,&nbsp;P Mancini ,&nbsp;G Bonanno Ferraro ,&nbsp;M Iaconelli ,&nbsp;C Del Giudice ,&nbsp;G La Rosa","doi":"10.1016/j.wroa.2023.100210","DOIUrl":"10.1016/j.wroa.2023.100210","url":null,"abstract":"<div><p>Influenza, a highly contagious acute respiratory disease, remains a major global health concern. This study aimed to comprehensively assess the prevalence of influenza virus in different aquatic environments.</p><p>Using 43 articles from four databases, we thoroughly examined water matrices from wastewater treatment plants (WTPs) and other human environments, as well as poultry habitats and areas frequented by migratory wild birds.</p><p>In WTP influents (10 studies), positivity rates for influenza A ranged from 0.0 % to 97.6 %. For influenza B (8 studies), most studies reported no positivity, except for three studies reporting detection in 0.8 %, 5.6 %, and 46.9 % of samples. Within poultry habitats (13 studies), the prevalence of influenza A ranged from 4.3 % to 76.4 %, while in environments frequented by migratory wild birds (11 studies), it ranged from 0.4 % to 69.8 %. Geographically, the studies were distributed as follows: 39.5 % from the Americas, 18.6 % from Europe, 2.3 % from South-East Asia and 39.5 % from the Western Pacific.</p><p>Several influenza A subtypes were found in water matrices, including avian influenza (H3N6, H3N8, H4N1, H4N2, H4N6, H4N8, H5N1, H5N8, H6N2, H6N6, H7N9, H0N8, and H11N9) and seasonal human influenza (H1N1 and H3N2). The existing literature indicates a crucial requirement for more extensive future research on this topic. Specifically, it emphasizes the need for method harmonization and delves into areas deserving of in-depth research, such as water matrices pertaining to pig farming and prevalence studies in low-income countries.</p></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":null,"pages":null},"PeriodicalIF":7.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589914723000464/pdfft?md5=b552d0126284fcf24d38f6beb5499ecf&pid=1-s2.0-S2589914723000464-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139013300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing ciprofloxacin removal through regulations of trophic modes and FNA levels in a moving bed biofilm reactor performing sidestream partial nitritation","authors":"Yifeng Xu ,&nbsp;Xi Wang ,&nbsp;Ying Gu ,&nbsp;Chuanzhou Liang ,&nbsp;Wenshan Guo ,&nbsp;Huu Hao Ngo ,&nbsp;Lai Peng","doi":"10.1016/j.wroa.2024.100216","DOIUrl":"https://doi.org/10.1016/j.wroa.2024.100216","url":null,"abstract":"<div><p>The performance of partial nitritation (PN)-moving bed biofilm reactor (MBBR) in removal of antibiotics in the sidestream wastewater has not been investigated so far. In this work, the removal of ciprofloxacin was assessed under varying free nitrous acid (FNA) levels and different trophic modes. For the first time, a positive correlation was observed between ciprofloxacin removal and FNA levels, either in the autotrophic PN-MBBR or in the mixotrophic PN-MBBR, mainly ascribed to the FNA-stimulating effect on heterotrophic bacteria (HB)-induced biodegradation. The maximum ciprofloxacin removal efficiency (∼98 %) and removal rate constant (0.021 L g⁻¹ SS h⁻¹) were obtained in the mixotrophic PN-MBBR at an average FNA level of 0.056 mg-N L⁻¹, which were 5.8 and 51.2 times higher than the corresponding values in the autotrophic PN-MBBR at 0 mg FNA-N L⁻¹. Increasing FNA from 0.006 to 0.056 mg-N L⁻¹ would inhibit ammonia oxidizing bacteria (AOB)-induced cometabolism and metabolism from 10.2 % and 6.9 % to 6.2 % and 6.4 %, respectively, while HB-induced cometabolism and metabolism increased from 31.2 % and 22.7 % to 41.9 % and 34.5 %, respectively. HB-induced cometabolism became the predominant biodegradation pathway (75.9 %-85.8 %) in the mixotrophic mode. Less antimicrobial biotransformation products without the piperazine or fluorine were newly identified to propose potential degradation pathways, corresponding to microbial-induced metabolic types and FNA levels. This work shed light on enhancing antibiotic removal via regulating both FNA accumulation and organic carbon addition in the PN-MBBR process treating sidestream wastewater.</p></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":null,"pages":null},"PeriodicalIF":7.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589914724000069/pdfft?md5=fb482c16d4af28cd1a078d1816daf628&pid=1-s2.0-S2589914724000069-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140041741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Underestimation of SARS-CoV-2 in wastewater due to single or double mutations in the N1 qPCR probe binding region","authors":"Jianxian Sun ,&nbsp;Minqing Ivy Yang ,&nbsp;Jiaxi Peng ,&nbsp;Ismail Khan ,&nbsp;Jhoselyn Jaramillo Lopez ,&nbsp;Ronny Chan ,&nbsp;Elizabeth A. Edwards ,&nbsp;Hui Peng","doi":"10.1016/j.wroa.2024.100221","DOIUrl":"https://doi.org/10.1016/j.wroa.2024.100221","url":null,"abstract":"<div><p>Wastewater surveillance using RT-qPCR has now been widely adopted to track circulating levels of SARS-CoV-2 virus in many sewersheds. The CDC qPCR assays targeting two regions (N1 and N2) within the N gene are commonly used, but a discrepancy between the two biomarkers has been noticed by independent studies using these methods since late 2021. The reason is presumed to be due to mutations in regions targeted by the N1 qPCR probe. In this study, we systematically investigated and unequivocally confirmed that the underlying reason for this discrepancy was mutations in the N1 probe target, and that a single mutation could cause a significant drop in signal. We first confirmed the proportion of related mutations in wastewater samples (Jan 2021-Dec 2022) using nested PCR and LC-MS. Based on relative proportions of N1 alleles, we separated the wastewater data into four time periods corresponding to different variant waves: Period I (Alpha and Delta waves with 0 mutation), Period II (BA.1/BA.2 waves with a single mutation found in all Omicron strains), Period III (BA.5.2* wave with two mutations), and Period IV (BQ.1* wave with two mutations). Significantly lower N1 copies relative to N2 copies in samples from Periods II-IV compared to those from Period I was observed in wastewater. To further pinpoint the extent to which each mutation impacted N1 quantification, we compared the qPCR response among different synthetic oligomers with corresponding mutations. This study highlighted the impact of even just one or two mutations on qPCR-based wastewater surveillance of SARS-CoV-2.</p></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":null,"pages":null},"PeriodicalIF":7.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589914724000112/pdfft?md5=cd6f9b015bef377ad09a98b6e6c6d53d&pid=1-s2.0-S2589914724000112-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140344689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}