Water Research X最新文献

Integrated social development on analyzing the distribution, risk and source apportionment of antibiotics pollution in mountainous rivers

IF 7.2 2区环境科学与生态学

Water Research X Pub Date : 2025-03-01 DOI: 10.1016/j.wroa.2025.100327

Wei Fan , Minjie Yang , Ying Shao , Dongjun Shen , Liang Ao , Zhongli Chen

{"title":"Integrated social development on analyzing the distribution, risk and source apportionment of antibiotics pollution in mountainous rivers","authors":"Wei Fan , Minjie Yang , Ying Shao , Dongjun Shen , Liang Ao , Zhongli Chen","doi":"10.1016/j.wroa.2025.100327","DOIUrl":"10.1016/j.wroa.2025.100327","url":null,"abstract":"<div><div>Antibiotics, as the widespread drugs stimulate the evolution of antibiotic resistance, threatening human and ecosystem health worldwide. However, studies rarely conducted in rivers among the regional scale with diverse economic development, which limits the management efficient of antibiotic control. Therefore, we investigated the concentration, distribution, risk and source apportionment of 54 antibiotics in 9 mountainous rivers, where the economic social development divers among their watersheds in Chongqing, China. The results showed that the concentrations of antibiotics detected in surface water, effluent of wastewater treatment plants, hospital, livestock and aquaculture sewage were 0.13–290 ng/L, 2.17–590 ng/L, 6.58–2.16 × 10<sup>5</sup> ng/L, 4.5–7.4 × 10<sup>5</sup> ng/L and 4.41–7.49 × 10<sup>3</sup> ng/L, respectively. The order of total antibiotic concentrations along the investigated rivers was Laixi River > Changshou Lake > Longxi River > Fujiang River > Jialing River > Qiongjiang River > Yangtze River > Wujiang River > Qijiang River. The risk quotient indicates that ofloxacin and lincomycin exhibited high risk. Through Mantel test and correlation analysis screened antibiotics associated with anthropogenic factors. Municipal wastewater had a positive impact on QNs. The positive matrix factorization model was used to identify the main sources of antibiotics in surface water with special focus on the Longxi River, revealing the livestock and aquaculture were main contributions, respectively. The ToxPi method was employed to prioritize antibiotics in surface water, and seven compounds were recommended as priority chemicals of concern in the future. This work provides a valuable regional scale dataset of antibiotics in the mountainous rivers, which promises valuable insights for controlling antibiotic contamination.</div></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":"28 ","pages":"Article 100327"},"PeriodicalIF":7.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upgrading waste activated sludge into valuable biosolids via an integrated biochemical approach

IF 7.2 2区环境科学与生态学

Water Research X Pub Date : 2025-02-27 DOI: 10.1016/j.wroa.2025.100325

Xi Lu , Zhiyao Wang , Zheng Kong , Haoran Duan , Zhiqiang Zuo , Zhetai Hu , Min Zheng , Shihu Hu

{"title":"Upgrading waste activated sludge into valuable biosolids via an integrated biochemical approach","authors":"Xi Lu , Zhiyao Wang , Zheng Kong , Haoran Duan , Zhiqiang Zuo , Zhetai Hu , Min Zheng , Shihu Hu","doi":"10.1016/j.wroa.2025.100325","DOIUrl":"10.1016/j.wroa.2025.100325","url":null,"abstract":"<div><div>Sludge management is a significant challenge for water utilities worldwide. High costs are largely driven by poor digestibility and dewaterability, while elevated concentrations of pathogens and toxic metals limit the safe and beneficial reuse of sludge. This study proposes an innovative integrated biochemical method that concurrently enhances sludge digestibility, facilitates toxic metal removal, improves dewaterability, and achieves pathogen reduction. In a laboratory-scale aerobic sludge digester processing waste-activated sludge, in-situ sludge acidification was achieved within 35–40 days, reaching a pH of approximately 2.6. This acidification was driven by the natural cultivation of acid-tolerant ammonia-oxidizing bacteria (<em>Ca.</em> Nitrosoglobus), which generate protons by oxidizing ammonium released from the sludge. Sludge acidification resulted in significant improvements, including volatile solids (VS) destruction (49 ± 6 %), pathogen reduction (∼4 log reduction), enhanced dewaterability (demonstrated by three methods), and toxic metal solubilization. While most toxic metals were solubilized to meet the Grade A biosolid standard (the highest biosolids standard qualified for unrestricted and safe use), copper only met the Grade B standard. To address this, a low dose of nitrite (5 mg N/L) was added to the acidified sludge (pH 2.2, adjusted with a small amount of acid) for 5 h, successfully solubilizing copper and upgrading the sludge to Grade A standards. Overall, this study demonstrates the potential of <em>in-situ</em> sludge acidification combined with minimal nitrite and acid addition as an efficient approach for improving multiple aspects of sludge management.</div></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":"28 ","pages":"Article 100325"},"PeriodicalIF":7.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the process performance of comammox-like nitrospira dominant down-flow hanging sponge reactor with reduced nitrous oxide emissions

IF 7.2 2区环境科学与生态学

Water Research X Pub Date : 2025-02-25 DOI: 10.1016/j.wroa.2025.100324

Sota Kabasawa , Takahiro Watari , Yuki Sato , Yuga Hirakata , Masashi Hatamoto , Tsutomu Okubo , Carols Lopez Vazquez , Jules B. van Lier , Takashi Yamaguchi

{"title":"Evaluation of the process performance of comammox-like nitrospira dominant down-flow hanging sponge reactor with reduced nitrous oxide emissions","authors":"Sota Kabasawa , Takahiro Watari , Yuki Sato , Yuga Hirakata , Masashi Hatamoto , Tsutomu Okubo , Carols Lopez Vazquez , Jules B. van Lier , Takashi Yamaguchi","doi":"10.1016/j.wroa.2025.100324","DOIUrl":"10.1016/j.wroa.2025.100324","url":null,"abstract":"<div><div>Nitrification/denitrification mitigates excess nitrogen in wastewater and reduces nutrient pollution in recipient surface waters but emits substantial amounts of nitrous oxide (N₂O). Complete ammonia-oxidizing (comammox) bacteria provide novel opportunities to mitigate N₂O emissions from wastewater treatment systems. In this study, a down-flow hanging sponge (DHS) reactor with low-strength ammonia-based synthetic wastewater was used to culture comammox bacteria, to study the microbial community structure, and to assess the nitrogen removal performance. The results showed a high NH<sub>4</sub><sup>+</sup>-N removal efficiency of 98 ± 4 % and complete nitrification during the entire experimental period. 16S rRNA gene sequencing and metagenomic analysis showed that comammox-like <em>Nitrospira</em> dominated the DHS-retained sludge, and that comammox-like <em>Nitrospira</em> and ammonia-oxidizing archaea may have coexisted symbiotically. The dissolved N₂O emissions per NH<sub>4</sub><sup>+</sup>-N removed from the DHS reactor were much lower than those from conventional activated sludge processes, indicating that the DHS reactor could be effective in reducing N₂O emissions during wastewater treatment.</div></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":"28 ","pages":"Article 100324"},"PeriodicalIF":7.2,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Making waves: Rethinking our mission for N2O emissions at WRRFs

IF 7.2 2区环境科学与生态学

Water Research X Pub Date : 2025-02-18 DOI: 10.1016/j.wroa.2025.100320

Leon Downing , McKenna Farmer , Bishav Bhattarai , Michael Penn , Joseph Kozak , Jonathan Grabowy , Fabrizio Sabba

{"title":"Making waves: Rethinking our mission for N2O emissions at WRRFs","authors":"Leon Downing , McKenna Farmer , Bishav Bhattarai , Michael Penn , Joseph Kozak , Jonathan Grabowy , Fabrizio Sabba","doi":"10.1016/j.wroa.2025.100320","DOIUrl":"10.1016/j.wroa.2025.100320","url":null,"abstract":"<div><div>Nitrous oxide (N<sub>2</sub>O) is a potent greenhouse gas with a global warming potential 273 times that of CO<sub>2</sub>, and it is a significant contributor to ozone depletion. Water resource recovery facilities (WRRFs) have been identified as a major source of N<sub>2</sub>O emissions, leading to significant research and policy efforts to mitigate these emissions. As WRRFs undertake these N<sub>2</sub>O mitigation efforts, important questions remain regarding the impact of more intensive nitrogen removal for pollution prevention and public health protection and how reactive nitrogen discharges are emitted as N<sub>2</sub>O in receiving waterways. To answer these questions, this perspective highlights the importance of balancing facility-scale emission factors to estimate N<sub>2</sub>O emissions from wastewater while considering the impacts of nitrogen if discharged to receiving water bodies. This perspective suggests more comprehensive approaches to manage N<sub>2</sub>O emissions, emphasizing the need to account for the reduction in N<sub>2</sub>O emissions achieved through nitrogen removal at WRRFs compared to direct discharge into receiving water bodies. By considering the overall impact of nitrogen from wastewater on N<sub>2</sub>O emissions from both WRRFs and receiving water bodies, WRRFs can reduce their impact on the environment while maintaining their important role in removing nitrogen from wastewater.</div></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":"28 ","pages":"Article 100320"},"PeriodicalIF":7.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New understanding of microbial growth cycle for efficient waste-activated sludge disposal by preserving microbial self-degradation activity during the decline phase

IF 7.2 2区环境科学与生态学

Water Research X Pub Date : 2025-02-17 DOI: 10.1016/j.wroa.2025.100323

Xiqi Li , Aijie Wang , Zhe Yu , Hualin Wang , Hengzhi You , Jifei Xu , Jingyu Zhang , Wenzong Liu

{"title":"New understanding of microbial growth cycle for efficient waste-activated sludge disposal by preserving microbial self-degradation activity during the decline phase","authors":"Xiqi Li , Aijie Wang , Zhe Yu , Hualin Wang , Hengzhi You , Jifei Xu , Jingyu Zhang , Wenzong Liu","doi":"10.1016/j.wroa.2025.100323","DOIUrl":"10.1016/j.wroa.2025.100323","url":null,"abstract":"<div><div>Diverse pretreatment methods have been developed for cell-cracking and sludge denaturation to harvest pretreated sludge as the suitable fermentation substrate, but new inoculation is not always working quickly or adaptively for the pretreated sludge. This study established a sludge SDMP method by integrating RL and HSHC to fully use the enriched microorganisms with decomposing enzymes/abilities at the sludge decline phase for organic matters bioconversion. The SDMP method cracked EPS but preserved and enhanced the microbial viability and the activity of enzymes related to hydrolysis and acidifying. Then the VFAs production cycle of SDMP sludge was shortened by 36.25 % than alkaline pretreated sludge, and the yield reached 4482.19 mg COD/L in 3 days with the organic matter conversion rate of 369.36 mg COD/g VSS. Preserved and enriched sludge self-degradation microorganisms composed a critical part of the dominant community, including <em>Mycobacterium, norank_f__Pirellulaceae, IMCC26207, norank_f__JG30-KF-CM45</em>, and <em>Petrimonas</em>. The interaction network centered on them facilitated acidogenic metabolism by reinforcing pathways such as glycolysis, pyruvate metabolism, and the Stickland reaction in amino acid metabolism. This study provides new insights into developing microbial functions for recycling resources from sludge.</div></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":"27 ","pages":"Article 100323"},"PeriodicalIF":7.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Layer-by-layer synthesis of copper hexacyanoferrate on 3D-printed scaffolds for efficient ammonium recovery

IF 7.2 2区环境科学与生态学

Water Research X Pub Date : 2025-02-16 DOI: 10.1016/j.wroa.2025.100322

Narges Dehbashi Nia , Bokseong Kim , Yuri Park , Yeo-Myeong Yun , Eveliina Repo , Yuhoon Hwang

{"title":"Layer-by-layer synthesis of copper hexacyanoferrate on 3D-printed scaffolds for efficient ammonium recovery","authors":"Narges Dehbashi Nia , Bokseong Kim , Yuri Park , Yeo-Myeong Yun , Eveliina Repo , Yuhoon Hwang","doi":"10.1016/j.wroa.2025.100322","DOIUrl":"10.1016/j.wroa.2025.100322","url":null,"abstract":"<div><div>Ammonium contamination in wastewater, which originates from various sources such as agriculture and livestock activities, poses significant environmental challenges while also serving as a valuable resource for recovery. Effective ammonium removal is essential for mitigating its impact on aquatic ecosystems, where it disrupts ecological balance and promotes toxic algal blooms. This study explores the potential of copper hexacyanoferrate (CuHCF), a widely recognized adsorbent among Prussian blue analogs (PBAs), for efficient ammonium adsorption due to its remarkable capacity and selectivity. CuHCF was immobilized on a three-dimensional (3D) printed scaffold using a layer-by-layer synthesis method, which significantly enhanced immobilization efficiency and adsorption performance compared to conventional single-layer methods. Analytical techniques, including Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), inductively coupled plasma mass spectrometry (ICP-MS), and thermogravimetric analysis (TGA), confirmed the introduction of carboxyl groups on the polylactic acid (PLA) scaffold through surface modification, enabling higher CuHCF loading. Adsorption tests revealed fast kinetics within 2 h, sustained adsorption performance for up to 10 days in continuous column experiments, and significant regeneration potential over five continuous cycles. These findings demonstrate the potential of the layer-by-layer synthesized CuHCF-immobilized filter for ammonium recovery from wastewater.</div></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":"28 ","pages":"Article 100322"},"PeriodicalIF":7.2,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Making waves: Generative artificial intelligence in water distribution networks: Opportunities and challenges

IF 7.2 2区环境科学与生态学

Water Research X Pub Date : 2025-02-13 DOI: 10.1016/j.wroa.2025.100316

Ridwan Taiwo , Abdul-Mugis Yussif , Tarek Zayed

{"title":"Making waves: Generative artificial intelligence in water distribution networks: Opportunities and challenges","authors":"Ridwan Taiwo , Abdul-Mugis Yussif , Tarek Zayed","doi":"10.1016/j.wroa.2025.100316","DOIUrl":"10.1016/j.wroa.2025.100316","url":null,"abstract":"<div><div>Water distribution networks (WDNs) face increasing challenges from aging infrastructure, population growth, and climate change, necessitating innovative technological solutions. This study examines the integration of Generative Artificial Intelligence (GenAI) in WDNs, including both conventional and reclaimed water systems. Through a comprehensive analysis of current literature and emerging applications, the study identifies key opportunities in near-future applications focusing on enhancing information retrieval through advanced document processing, improving water quality management via real-time monitoring and visualization, implementing predictive maintenance strategies through pattern recognition, and optimizing real-time operational control through adaptive algorithms. Results also demonstrate that GenAI can transform WDN operations through advanced visualization, scenario generation, and adaptive optimization capabilities, particularly in far-future applications such as demand forecasting, emergency response, and network design optimization. The analysis reveals significant challenges, including data quality and availability issues, particularly in non-English speaking regions, scalability constraints in large-scale networks, the critical need for water professionals with hybrid expertise in both traditional engineering and AI systems, and complex regulatory requirements that vary significantly across the globe. The study also explores unique applications in reclaimed WDNs, particularly in quality control, treatment optimization, and stakeholder engagement. These findings provide water utilities, policymakers, and researchers with valuable insights for implementing GenAI technologies while balancing technological advancement with human expertise and social responsibility.</div></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":"28 ","pages":"Article 100316"},"PeriodicalIF":7.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lake dissolved organic matters seasonal variations is a main driver of N2O emission: In molecular insights by using FT-ICR MS

IF 7.2 2区环境科学与生态学

Water Research X Pub Date : 2025-02-13 DOI: 10.1016/j.wroa.2025.100321

Zezheng Wang , Lu Li , Chengchao Liao , Min Deng , Yeerken Senbati , Yongxia Huang , Kang Song

{"title":"Lake dissolved organic matters seasonal variations is a main driver of N2O emission: In molecular insights by using FT-ICR MS","authors":"Zezheng Wang , Lu Li , Chengchao Liao , Min Deng , Yeerken Senbati , Yongxia Huang , Kang Song","doi":"10.1016/j.wroa.2025.100321","DOIUrl":"10.1016/j.wroa.2025.100321","url":null,"abstract":"<div><div>Anthropogenic activities have introduced substantial quantities of carbon and nitrogen into aquatic system, which are hotspots for nitrous oxide emissions and play multiple roles in the global biogeochemical cycle. We characterized the seasonal variation of DOM by employing a comprehensive approach incorporating absorption spectroscopy, excitation-emission matrix fluorescence and Fourier transform ion cyclotron resonance mass spectrometry (FT–ICR MS). Results revealed that DOM seasonal variation could be the main factor driven lake N<sub>2</sub>O emission alteration. In warm seasons, lakes are predominant with recalcitrant aromatic compounds DOM released by phytoplankton, such as highly unsaturated and phenolic compounds, polyphenols., and lignin, accompanied by low N<sub>2</sub>O concentrations (0.03±0.02 μmol/L). In cold seasons, bioavailable components (Aliphatic, Sugar-like, Peptide-like) are dominated in lakes, the increased abundance of S-containing compounds and low aromaticity compounds largely influenced by anthropogenic emissions, leads to elevated N<sub>2</sub>O concentrations (1.91±0.06 μmol/L). The DOM with high bioavailable components promotes sediment N<sub>2</sub>O production (<em>nir</em>/<em>nosZ (<sub>I+II)</sub>-</em>type denitrifiers) (<em>n</em> = 678). Recalcitrant DOM reduced sediment <em>nirK</em> and <em>nirS-</em>type denitrifiers, and increased <em>nosZ <sub>(I+II)</sub></em>-type denitrifiers, enhance the N<sub>2</sub>O reduction and led to a lower N<sub>2</sub>O emission. This study advances the understanding of the microbial mechanisms that DOM regulates N<sub>2</sub>O emissions in lakes.</div></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":"28 ","pages":"Article 100321"},"PeriodicalIF":7.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of acid-tolerant ammonia-oxidizing Nitrosospira bacteria suggests wide implementation of the acidic nitritation process

IF 7.2 2区环境科学与生态学

Water Research X Pub Date : 2025-02-10 DOI: 10.1016/j.wroa.2025.100317

Zhetai Hu , Xiaotong Cen , Zhiyao Wang , Min Zheng

{"title":"Characterization of acid-tolerant ammonia-oxidizing Nitrosospira bacteria suggests wide implementation of the acidic nitritation process","authors":"Zhetai Hu , Xiaotong Cen , Zhiyao Wang , Min Zheng","doi":"10.1016/j.wroa.2025.100317","DOIUrl":"10.1016/j.wroa.2025.100317","url":null,"abstract":"<div><div>Acidic activated sludge process has been recently developed for the suppression of nitrite-oxidizing bacteria, addressing the bottleneck in achieving robust partial nitritation (PN) for low-strength ammonia wastewater treatment. In the acidic process, three types of ammonia-oxidizing bacteria (AOB) have been detected, including acid-sensitive neutrophilic <em>Nitrosomonas</em>, acid-tolerant neutrophilic <em>Nitrosospira</em>, and acidophilic <em>Candidatus</em> (<em>Ca.</em>) <em>Nitrosoglobus</em>. Among them, <em>Nitrosospira</em> is much less studied. This study successfully enriched <em>Nitrosospira</em> in a membrane bioreactor at a pH of around 5.3. Stoichiometric and kinetic characterizations of the enriched <em>Nitrosospira</em> culture were determined, including yield, maximum growth and decay rates, affinity for oxygen and total ammonia, and susceptibility to common influencing factors such as pH, nitrite, free nitrous acid (FNA), salinity, and temperature. The results indicated that, compared to <em>Nitrosomonas, Nitrosospira</em> has a higher tolerance to acidity and FNA but a lower maximum growth rate. <em>Nitrosospira</em> has a competitive advantage over acidophilic AOB under neutral, oxygen-depleted, high-salinity or low-temperature conditions. These findings advance our understanding of utilising <em>Nitrosospira</em> for the acidic PN process, indicating a wider implementation of the process under these conditions.</div></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":"28 ","pages":"Article 100317"},"PeriodicalIF":7.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterogeneous microstructure induces floatation in high-rate anammox granules

IF 7.2 2区环境科学与生态学

Water Research X Pub Date : 2025-02-10 DOI: 10.1016/j.wroa.2025.100319

Da Kang , Huifeng Lu , Tingting Kang , Yihan Zhang , Zheng Ge , Liang Zhang , Yongzhen Peng

{"title":"Heterogeneous microstructure induces floatation in high-rate anammox granules","authors":"Da Kang , Huifeng Lu , Tingting Kang , Yihan Zhang , Zheng Ge , Liang Zhang , Yongzhen Peng","doi":"10.1016/j.wroa.2025.100319","DOIUrl":"10.1016/j.wroa.2025.100319","url":null,"abstract":"<div><div>The floatation of anammox granules can be a serious challenge in practical wastewater treatment, as it can deteriorate reactor performance and cause bacterial loss. To deepen the understanding of floatation mechanism, in this study, both the floating (F-AnGS) and settling anammox granules (S-AnGS) from a high-rate anammox reactor were comparatively investigated. F-AnGS demonstrated 1.6 times higher specific anammox activity compared to S-AnGS, but only 65 % of produced gas could be successfully released, as quantified by anaerobic respirometry. In addition to the overall EPS accumulation, F-AnGS exhibited a heterogeneous microstructure distinct from that of S-AnGS, as revealed by 3D X-ray microscopic imaging at the single granule level. The heterogeneous distribution of EPS, which can form a dense surface layer, was the main cause for granule floatation. The heterogeneous microstructure of F-AnGS can reduce the distance between microorganisms and enhance the metabolic interaction between anammox bacteria and heterotrophs. The abundance of community members did not have a significant variation, but the functional genes related to anammox and partial denitrification pathway were significantly increased, indicating the enhanced nitrite loop in F-AnGS. This study proposed new structural insights into mechanism of anammox granule floatation, suggesting the appropriate activity control of granule-based anammox process.</div></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":"28 ","pages":"Article 100319"},"PeriodicalIF":7.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0