Cellular and molecular biology (Noisy-le-Grand, France)最新文献

{"title":"Molecular mechanisms underlying the antitumor activity of human and bovine alpha-lactalbumin-oleic acid complexes in a murine mammary adenocarcinoma model.","authors":"Muataz Mohammed Al-Taee","doi":"10.14715/cmb/2025.71.7.11","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.11","url":null,"abstract":"<p><p>The alpha-lactalbumin-oleic acid complexes (BAMLET and HAMLET) derivatives have shown remarkable anticancer capabilities in various preclinical studies with potential applications in oncology. The current study investigates the anti-cancer activity of synthesized human alpha-lactalbumin oleic acid (HAMLET) and bovine alpha-lactalbumin oleic acid (BAMLET) complexes on AN3 mouse mammary adenocarcinoma cancer cell line, a long-standing model for cancer research. We investigated multiple therapeutic endpoints including tumor volume reduction, survival rates, histopathological changes, as well as the molecular mechanisms allying the treatment response. A significant suppression of tumor growth was observed in both groups treated with HAMLET and BAMLET when compared with the control group, with HAMLET showing slightly better effectiveness in tumor growth inhibition. Histological examination revealed tumor necrosis, apoptosis, and decreased cell proliferation in treated mice, cancer cells were dying and also growth architecture was disrupted which indicates that both complexes cancer cell death. Thus, we investigated major molecular pathways associated with the anticancer activity of these compounds. Findings revealed the activation of supportive apoptotic pathways alongside downregulation of fundamental oncogenes linked to growth endurance and metastasis. Likewise, the safe response was augmented in the treated groups as shown by greater infiltration of immune cells into the tumor microenvironment. Survival rates were significantly higher in the HAMLET and BAMLET treatment groups compared to control, suggesting that these assemblies may prolong survival by effectively reducing cancer burden. The results highlight once again the exceptional breast cancer treatment efficacy of the alpha-lactalbumin-oleic acid complex.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"81-86"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of matrine on cardiomyocytes infected with coxsackievirus B₃ via modulation of the calpain-2/caspase-12 signaling pathway.","authors":"Yongmei Sun, Zongtao Mao, Junzuo Liu, Yanan Geng","doi":"10.14715/cmb/2025.71.7.4","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.4","url":null,"abstract":"<p><p>Viral myocarditis (VMC) presents a substantial threat, especially for children, often leading to cardiogenic shock and fulminant myocarditis. Our study aimed to evaluate the role of calpain-2 and caspase-12, which were involved in the endoplasmic reticulum apoptosis pathway, and the influence of Matrine on these proteins during Coxsackie virus B₃ (CVB₃)-induced acute VMC mice in vitro and in vivo, shedding light on the potential cardioprotective effects. We first performed primary cultured cardiomyocytes, which were infected with CVB₃in vitro. We observed cell viability, the beating of cardiomyocytes and cytopathic effects. And we utilized Balb/c mice to establish the VMC animal model and determined viral titers, histopathological changes, and myocardial pathological scores. Furthermore, we detected CK-MB levels and myocardial cell apoptosis in vitro and in vivo. In order to further explore the possible mechanisms, the protein expression of calpain-2 (by immunohistochemistry and Western blot) and caspase-12 activity (by fluorescence assay for substrate cleavage) were detected in vitro and in vivo. Our findings indicated that, in comparison to the normal control group, the virus-infected group exhibited increased injured myocardial cells, virus titer, CK-MB levels, and apoptotic cells (P<0.05). Matrine treatment groups significantly reduced CK-MB levels, myocardial cellular damages and apoptosis in vitro and in vivo, with Matrine notably suppressing calpain-2 protein expression and Caspase-12 activity compared to the virus-infected group (P<0.05). In conclusion, our study revealed that calpain-2 and caspase-12 played roles in CVB₃-induced myocardial cell apoptosis. Matrine effectively mitigated myocardial cell injury and reduced apoptosis, thereby providing substantial protection against CVB₃infection in vitro and in vivo, which may be related to the down-regulation of calpain-2/caspase-12 signaling pathway.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"21-30"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequencing of TNFα and IFNϒ genes associated with Toxoplasma gondii infection among Erbil residents-Iraq.","authors":"Ahmed Akil Khudhair Al-Daoody","doi":"10.14715/cmb/2025.71.7.14","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.14","url":null,"abstract":"<p><p>Toxoplasmosis is a serious disease that affects all age groups and may even threaten the lives of some people. Fifty serum samples were taken from patients has Toxoplasmosis who attended Rizgary Teaching Hospital in Erbil City, and 38 serum samples were taken from healthy individuals as a control group, with ages between 18-60 years old, from the period January 2024 to March 2025. The purpose of the research is to determine the gene sequence of TNFα and IFNϒ associated with Toxoplasmosis. The results showed that the infection prevalence rate in the age group (18-27) years was 17 (56.7%) in comparison to healthy individuals 13 (43.3%), and in the age group (28-37) years was 20 (62.5%) compared to healthy control 12 (37.5%), and in the age group (38-47) years was 10 (76.9%) in comparison to the controls 3 (23.1%), while in the age group (≥47) years was 3 (23.1%) compared to the controls 10 (76.9%) with significant variations (P=0.03). The prevalence rate of Toxoplasmosis in males was 17 (48.6%) in comparison to the healthy controls 18 (51.4%), while in females it was 33 (62.3%) compared to the healthy individuals 18 (51.4%), with no significant variations (P=0.29). The mean level of Toxoplasmosis IgM was (2.28±0.18) when compared to healthy group (0.12±0.03), and the mean level of Toxoplasmosis IgG was (2.12±0.18) when compared to healthy control (0.09±0.02). The mean level of TNF-α (pg/ml) was (10.34±0.39) in comparison to the control group (4.89±0.31), and the mean level of INF-γ (pg/ml) was (10.72±0.36) in comparison to the controls (4.80±0.29) with highly significant variations (P=0.001).  Moreover, direct correlation between Toxoplasma IgM with IgG was (r=.568), with TNF-α was (r=.607), with INF-γ was (r=.528), with highly significant variations P= (.000, .000, .000) respectively. Also, there were direct correlations between Toxoplasma IgG with IgM (r=1), with TNF-α (r=.550), with INF-γ (r=.576), with highly significant variations P= (.000, .000, .000) respectively. The TNFα gene sequence ID 7132 of rs767455 in the position AA was changed to AG and GG, respectively, in samples 1-10 in comparison to the control group. Also, the INFϒ gene sequence ID 3458 of rs2430561 in the position TT was changed to TA and AA, respectively, in samples 1-10 in comparison to the control group.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"102-107"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the prospects of new therapeutic agents for the treatment of rheumatoid arthritis.","authors":"Alexander V Blagov, Nikolay A Orekhov, Dmitry F Beloyartsev, Alexey V Churov, Tatiana I Kovyanova, Irina A Starodubtseva, Vasily N Sukhorukov, Alexander N Orekhov","doi":"10.14715/cmb/2025.71.7.2","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.2","url":null,"abstract":"<p><p>Rheumatoid arthritis (RA), a chronic autoimmune disease, is one of the major research themes in medicine. The current therapies have their limitations and cannot completely cure RA, but new therapeutic strategies are being proposed to reduce the shortcomings of approved drugs. This review will consider new potential treatment strategies for RA, including T-cell therapy, genetic editing and epigenetic regulation, what advantages and disadvantages they have and to what pathological target in RA they are directed.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"8-15"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotoxic of co-codamol for human lymphocyte culture in vitro.","authors":"Sada Jasim Abdulameer, Anfal Izaldeen Alkateeb, Layth Ammar Chyad Al-Shammari","doi":"10.14715/cmb/2025.71.7.3","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.3","url":null,"abstract":"<p><p>Co-codamol, a combination analgesic containing paracetamol and codeine phosphate, is widely used for pain relief, but its potential genotoxic effects on human lymphocytes remain largely unknown. This in vitro study investigates the genotoxic potential of co-codamol on cultured human lymphocytes by assessing cell viability, mitotic index (MI), chromosomal aberration frequency, and micronucleus (MN) formation. Lymphocytes were exposed to varying concentrations of co-codamol (0.02-0.12 mg/mL), and cytotoxicity was determined using the MTT assay. Results showed that co-codamol significantly reduced cell viability in a dose-dependent manner, with complete cell death at 0.12 mg/mL. The mitotic index was significantly decreased at higher concentrations, and a statistically significant increase in chromosomal aberrations and micronucleus formation was observed in treated lymphocytes compared to the control group (p < 0.05). These findings provide important evidence that co-codamol exhibits genotoxic potential in vitro, suggesting a potential risk of DNA damage associated with its use. Further in vivo investigations are warranted to assess the clinical relevance of these genotoxic effects and to elucidate the underlying mechanisms of toxicity.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"16-20"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LINC01232 regulates miR-516a-5p/BCL9 axis to promote triple-negative breast cancer progression.","authors":"Wei Liu, Yunfeng Niu, Jie An","doi":"10.14715/cmb/2025.71.7.10","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.10","url":null,"abstract":"<p><p>Triple-negative breast cancer (TNBC) is characterised by an absence of the oestrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2), for which there are few therapeutic options and the prognosis is poor. This research sought to explore the particular function of the long non-coding RNA (lncRNA) LINC01232 in TNBC and its regulatory impacts on the miR-516a-5p/BCL9 pathway. In this study, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to determine the expression level of LINC01232 in TNBC tissues. We also examined its regulatory influences on miR-516a-5p and BCL9 via cellular function tests and a luciferase reporter experiment. Evaluated the effect of LINC01232 silencing on proliferation, migration and invasion of breast cancer cells. Results showed that LINC01232 expression was abnormally high in TNBC tissues in comparison to normal tissues. Inhibition of LINC01232 expression markedly impeded breast cancer cell proliferation, clone formation, migration and invasion. We found that LINC01232 competes with miR-516a-5p for binding, thereby reducing its expression and subsequently increasing BCL9 expression. In conclusion, our results indicate that LINC01232 facilitates the malignant development of TNBC through the miR-516a-5p/BCL9 pathway, providing fresh perspectives on the pathogenesis of TNBC and pinpointing potential therapeutic targets.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"72-80"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rutin treatment alleviates obesity-related aortic endothelium dysfunction in albino rats fed a high-fat diet.","authors":"Hassan A Madkhali, Majid A Ganaie, Mohd Nazam Ansari, Najeeb Ur Rehman, Abubaker M Hamad, Gamal A Soliman, Khalid F Alanazi, Mohammed M Ahmed, Abdullah Y Hamadi, Naif M Alhawiti","doi":"10.14715/cmb/2025.71.7.5","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.5","url":null,"abstract":"<p><p>Flavonoids have recently been shown to be useful to people suffering from vascular disorders caused by a high-fat diet (HFD). The flavonoid rutin (RT) exhibits numerous pharmacological effects, including antioxidant, cytoprotective, vasoprotective, and cardioprotective activities. The primary objective of this research was to assess the efficacy of RT against obesity-related vascular endothelial dysfunction (VED) in rats fed HFD. A total of 24 mature Wistar rats were blindly categorized into 4 treatment and control groups: normal control, obese control, and obese which were given RT at 50 and 100 mg/kg for the final 3 weeks of the experimental period. Animals' body mass and food consumption have been estimated periodically. In addition, liver mass and retroperitoneal fat mass per body mass, abdominal circumference (AC), LEE index, and body mass index (BMI) were estimated. Moreover, lipid profile parameters were assessed in serum. The effect on vascular endothelium reactivity was investigated in an isolated rat aorta. A histopathological investigation of the aorta was performed. The obese control group exhibited higher body, liver, and retroperitoneal fat weights. Significantly, RT intake reverses all these alterations. Furthermore, RT decreased food intake, AC, Lee index, and BMI in HFD-fed rats. The lipid profile of HFD-fed rats was also improved after RT treatment, with lower triglycerides, total cholesterol, LDL-C, and VLDL-C levels and higher HDL-C levels in the serum of HFD-fed rats. Through the ex-vivo investigation, RT groups showed improved vascular endothelium function in HFD-fed animals compared to the obese control group. Taking together, RT could be a promising option for preventing obesity-associated VED.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"31-38"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modern methods of detecting mitophagy.","authors":"Alexander V Blagov, Andrey Y Vinokurov, Vasily N Sukhorukov, Anton Y Postnov, Elizaveta M Pleshko, Alexander N Orekhov","doi":"10.14715/cmb/2025.71.7.1","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.1","url":null,"abstract":"<p><p>Inhibition of mitophagy is one of the signs of chronic disease pathogenesis. Detection and measurement of mitophagy levels under in vitro and in vivo models provide a better understanding of the role of mitophagy disorder in disease development and serve as prerequisites for creating a clinically applicable system test. The development of such a system is potentially feasible, but taking into account a number of factors that will be discussed in detail in this article. Here it is considered the main models of mitophagy-based test systems and an analysis is carried out showing their advantages and disadvantages. The future potential for the development of mitophagy-based diagnostic test systems is also discussed here.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular evaluation of quercetin effects in a murine model of giant cell tumor of bone: an in vivo pilot study.","authors":"Dalia Lizbeth Monroy-Quiroz, Alexandra Berenice Luna-Angulo, Brandon Eduardo Galicia-Canales, Laura Sánchez-Chapul, Olivia Hernández-González, María Del Rocío Aguilar-Gaytán, Mónica Guadalupe Santamaría-Olmedo, Alberto Hidalgo-Bravo, Beatriz Del Carmen Couder-García, Gabriel Lara-Hernández, Erendira Georgina Estrada-Villaseñor, Carlos Landa-Solís","doi":"10.14715/cmb/2025.71.7.15","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.15","url":null,"abstract":"<p><p>Quercetin, a flavonoid derived from plant sources, has been extensively studied for its numerous biological properties, particularly its potential antitumor action against various malignant neoplasms. In our experience with a giant cell tumor of bone cell line (TIB-223), we demonstrated that quercetin has the ability to induce apoptosis via caspase-3. Therefore, this study aimed to evaluate molecular markers for apoptosis, necrosis, and cell proliferation in a murine model of giant cell tumor of bone, to determine whether the behavior reported for quercetin in 2D remains consistent in a 3D in vivo tumor model. Tumor constructs based on TIB-223 cells were implanted into athymic mice, and two weeks post-implantation, the mice were orally administered quercetin at a concentration of 100 mg/kg body weight once a day for two weeks. The control group received only 200 µL of the vehicle. Our results demonstrate the activation of two cell death pathways in the implanted tumors: apoptosis, via Caspase-8 to Caspase-3 activation, and necroptosis, via RIPK1. No significant effect on cell proliferation was observed, as PCNA expression remained unchanged. Our results suggest that quercetin may induce specific mechanisms of cell death without significantly altering cell proliferation in the tumor model induced in mice.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"108-113"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical screening and evaluation of antibacterial and antifungal activities of Callistemon viminalis cultivated in Iraq.","authors":"Zaineb Aziz Ali, Fatimah Ahmed Challoob Al-Khuzaee, Yasser Kadhim Hashem Al-Zwaini, Mustafa Mohammed Albassam, Rwaieda Adil Muhsen, Balqess Hisham Salih, Mohammed Hasan Kadhim, Sumia Samer Tayeh","doi":"10.14715/cmb/2025.71.7.12","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.12","url":null,"abstract":"<p><p>Callistemon viminalis, a member of the Myrtaceae family, is traditionally used to treat infections, respiratory, and gastrointestinal disorders. This study aimed to investigate the phytochemical profile and evaluate the antibacterial and antifungal activities of C. viminalis leaves cultivated in Iraq. Leaves were collected, dried, and extracted sequentially with hexane and 70% ethanol. Preliminary phytochemical screening revealed the presence of saponins, terpenoids, alkaloids, and flavonoids. The antimicrobial activity of the ethanol extract was assessed using the agar well diffusion method against three Gram-positive bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus sp.), two Gram-negative bacteria (Escherichia coli, Klebsiella sp.), and one fungal strain (Candida albicans). The ethanol extract exhibited notable inhibitory effects, particularly against Gram-positive bacteria and C. albicans, with activity increasing in a concentration-dependent manner. GC-MS analysis of the hexane extract identified key bioactive compounds, including beta-sitosterol and vitamin E. These findings highlight the significant pharmacological potential of C. viminalis leaves and support their traditional use as a source of natural antimicrobial agents. Further studies are recommended to isolate and characterize the active constituents responsible for these effects.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"87-91"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}