Cellular and molecular biology (Noisy-le-Grand, France)最新文献_第4页

Global reports on Glutamicibacter: a mini review. 谷氨酰胺菌全球报告：一个小回顾。

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2026-02-28 DOI: 10.14715/cmb/2025.72.2.2

VarshaVarthini Ganesapandi, Rangasamy Kavitha

{"title":"Global reports on Glutamicibacter: a mini review.","authors":"VarshaVarthini Ganesapandi, Rangasamy Kavitha","doi":"10.14715/cmb/2025.72.2.2","DOIUrl":"https://doi.org/10.14715/cmb/2025.72.2.2","url":null,"abstract":"Glutamicibacter has been recently reclassified from the genus Arthrobacter and represents a rare group of actinobacteria with significant biotechnological potential. Based on 16S rRNA signatures and phylogenomic evidence, its species have been discovered in diverse habitats including soils, sediments, wetlands, permafrost, saline coasts, and deserts. Ecologically, they act as endophytes, rhizosphere partners, and pioneers in extreme environments, reflecting exceptional resilience. These rare actinobacteria exhibit distinct biochemical profiles and follow a rod-coccus life cycle dependent on growth phase. Genomic surveys reveal compact genomes containing biosynthetic gene clusters (BGCs) that encode novel metabolites. Functionally, Glutamicibacter produces cold-activated and thermoalkaliphilic enzymes, antioxidant exopolysaccharides, uric acid-degrading pathways, and antimicrobial compounds. They have applications in food safety, agriculture, bioremediation, and therapeutics. However, BGCs remain uncharacterized in most species, and mechanisms underlying plant-microbe and insect-microbe interactions are poorly understood. Additionally, the industrial development of their enzymes and metabolites is limited. This review integrates contemporary perspectives on the taxonomy, ecology, morphology, genomics, and metabolism of Glutamicibacter and identifies future prospects through genome mining, metabolomics, and synthetic biology.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 2","pages":"7-13"},"PeriodicalIF":0.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148298740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulmonary anthracosis in a free-ranging infant rhesus monkey (Macaca mulatta) in Bangladesh- A Case Report. 孟加拉自由放养的恒河猴幼猴（Macaca mulatta）的肺炭疽病- 1例报告。

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2026-02-28 DOI: 10.14715/cmb/2025.72.2.1

Rasel Prank, Asm Lutful Ahasan, Syed Hossain, Subrata Kumar Shil

{"title":"Pulmonary anthracosis in a free-ranging infant rhesus monkey (Macaca mulatta) in Bangladesh- A Case Report.","authors":"Rasel Prank, Asm Lutful Ahasan, Syed Hossain, Subrata Kumar Shil","doi":"10.14715/cmb/2025.72.2.1","DOIUrl":"10.14715/cmb/2025.72.2.1","url":null,"abstract":"The study aimed to describe a rare case of pulmonary anthracosis in a free-ranging infant rhesus monkey in Bangladesh. An infant Rhesus monkey (Macaca mulatta) just under 1 year of age, was admitted to a veterinary hospital in Bangladesh with clinical signs including loss of appetite, gradual weight loss, weakness, dehydration, depression, breathing difficulty, and mild nasal discharge. The animal died suddenly within 24 hours of admission. A necropsy was performed on the infant monkey, and tissue samples were collected and sent to the Department of Anatomy and Histology at Chittagong University of Veterinary and Animal Sciences (CVASU) for histopathological analysis. Gross examination revealed numerous uniformly distributed black spots in both lungs. The lungs showed significant inflammation, were firm, swollen, and abnormally colored, but no nodules or fibrosis were observed. Microscopic examination revealed black carbon particles, inflammatory cells, edema fluid, and brown hemosiderin-containing macrophages in the pulmonary interstitium. Diffuse alveolar damage with exudate and carbon particles was also observed. The infant monkey died from cardiopulmonary failure due to chronic respiratory distress. The presence of pulmonary carbonization indicates poor air quality in the animal's habitat, which may pose a threat to the health of both wildlife and humans.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 2","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148298778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized enzymatic cell lysis protocols for single-cell genomic analysis. 优化单细胞基因组分析的酶细胞裂解方案。

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2026-02-13 DOI: 10.14715/cmb/2025.72.1.5

Houssam Raad, Hamid Bou Saab, Ahmad Al Saabi, Hassan Ayoub, Hussein Mouawia

引用次数: 0

Screening for MSX1 and PAX9 gene variants among hypodontia patients in the Jordanian population. 约旦人口下颌畸形患者中MSX1和PAX9基因变异的筛查

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2026-02-13 DOI: 10.14715/cmb/2025.72.1.6

Areej Quran, Ahmed Maslat, Saied Jaradat, Eman Hammouri, Abeer Quran

{"title":"Screening for MSX1 and PAX9 gene variants among hypodontia patients in the Jordanian population.","authors":"Areej Quran, Ahmed Maslat, Saied Jaradat, Eman Hammouri, Abeer Quran","doi":"10.14715/cmb/2025.72.1.6","DOIUrl":"10.14715/cmb/2025.72.1.6","url":null,"abstract":"This study aimed to sequence the PAX9 and MSX1 genes in a group of Jordanian patients diagnosed with non-syndromic hypodontia. The study also examined whether the identified genetic variants in these key odontogenic genes could be used to assess susceptibility to hypodontia. Fifty Jordanian individuals diagnosed with non-syndromic hypodontia were enrolled in this study. Genomic DNA was extracted, and all exons of PAX9 (Exons 1,2, 3, and 4) and MSX1 (Exons 1 and 2) were amplified using PCR. The Sanger sequencing technique was used to detect genetic variants, which were analyzed for their clinical significance and evolutionary conservation. Several previously reported genetic variants were detected. In MSX1, two missense variants, c. 77G>C; p. (Gly26Ala)(rs567549350) and c. 119C>G; p. (Ala40Gly)(rs36059701), and a regulatory variant, c. *6C > T(rs8670), were identified. In PAX9, six genetic variants were found: c. 5-109G>C (rs12883298), c. 5-54A>G (rs1288923), c. 5-41A>G(rs12883049), c. 631+41G>A(rs2236007), c. 717C>T; p. (His239His)(rs12881240) and c. 718G>C; p. (Ala240Pro)(rs4904210). Additionally, the c. *6C > T (rs8670) variant in MSX1 was associated with upper lateral incisor agenesis. The c. -18G>A (rs186861426) variant in MSX1 was evolutionarily conserved across multiple species, suggesting a potential regulatory role in odontogenesis. This study confirmed the presence of several previously reported PAX9 and MSX1 variants among Jordanian individuals with hypodontia. The findings emphasize the importance of genetic screening for hypodontia but suggest that the identified variants are likely benign polymorphisms rather than causative variants. Further studies with larger sample sizes and functional analyses are needed to determine the regulatory impact of non-coding variants and their role in tooth agenesis.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 1","pages":"39-46"},"PeriodicalIF":0.0,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146184142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in algal biofuel production through catalysts and nanocatalysts: an integrated chemical, biochemical, and physical analysis. 通过催化剂和纳米催化剂生产藻类生物燃料的进展：综合化学、生化和物理分析。

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2026-02-13 DOI: 10.14715/cmb/2025.72.1.8

Anis Ahmad Chaudhary

{"title":"Advances in algal biofuel production through catalysts and nanocatalysts: an integrated chemical, biochemical, and physical analysis.","authors":"Anis Ahmad Chaudhary","doi":"10.14715/cmb/2025.72.1.8","DOIUrl":"10.14715/cmb/2025.72.1.8","url":null,"abstract":"Biofuel has emerged as one of the most promising alternatives to petroleum-derived fuels, particularly in the current context of rapidly depleting fossil fuel reserves. The combustion of fossil-based fuels poses serious risks to both the environment and human health due to the release of greenhouse gases. This study aimed to assess the fatty acid methyl ester (FAME) composition, viscosity, acid number, and chemical metal elements present in algal biodiesel produced through a transesterification bioprocess. Nanoparticles (NPs) offer a promising solution to current challenges in biomass utilization. Their advantages include high selectivity, energy efficiency, effective time management, and reduced costs. Biodiesel conversion yield, along with its physical and chemical properties, was analyzed using GC-MS and a multi-element oil analyzer. The highest conversion yield of 98.9% was achieved using a mixed catalyst-nanocatalyst system (NaOH + CaO + ZnO) under optimal conditions: a 1:4 oil-to-methanol volumetric ratio, 1.0% catalyst concentration, reaction temperature of 40°C, and stirring speed of 320 rpm. A significant difference was observed in the viscosity and acid value between biodiesel produced using a single catalyst and that produced using catalyst mixtures. Furthermore, the concentrations of chemical metal elements (Na, Ca, Mg, Cu, Fe, Zn, Ag, Al, P, Pb, B, Cr, Mn, Sn, Mo, Si, Ba, Ni, Ti, and V) were lower in biodiesel derived from mixed nanocatalyst-catalyst combinations (NaOH + ZnO, NaOH + CaO, and NaOH + CaO + ZnO) compared to the single-catalyst system (NaOH). Overall, biodiesel produced using the combined catalyst and nanocatalyst approach exhibited reduced metal element content and superior yield compared to biodiesel obtained using a single catalyst. In addition, nanomaterials such as nanocatalysts have been found to be cost effective as well as stable catalysts for enzyme immobilization, thus improving biofuel synthesis.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 1","pages":"53-58"},"PeriodicalIF":0.0,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146184156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinicopathological Characteristics of PRRX1 and PRRX2 Genes Expressions in Colorectal Cancer Patients. 结直肠癌患者PRRX1和PRRX2基因表达的临床病理特征

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2026-02-13 DOI: 10.14715/cmb/2025.72.1.7

Samira Zaman Nezhad, Zahra Mozooni, Amirhossein Eskandari, Alireza Ahmadzadeh, Zihab Sohbatzadeh, Ali Delpisheh, Mehrdad Hashemi, Abolfazl Movafagh

{"title":"Clinicopathological Characteristics of PRRX1 and PRRX2 Genes Expressions in Colorectal Cancer Patients.","authors":"Samira Zaman Nezhad, Zahra Mozooni, Amirhossein Eskandari, Alireza Ahmadzadeh, Zihab Sohbatzadeh, Ali Delpisheh, Mehrdad Hashemi, Abolfazl Movafagh","doi":"10.14715/cmb/2025.72.1.7","DOIUrl":"10.14715/cmb/2025.72.1.7","url":null,"abstract":"Colorectal cancer (CRC) ranks as the third leading cause of cancer-related deaths globally. It results from polyp growth or ulcers formation in the colon or intestine lining. Genetic, environmental factors, unhealthy eating habits, and lifestyle choices contribute to CRC development. Genetic and epigenetic changes play a crucial role in tumor development. Homeobox (HOX) genes contain the homeodomain, encoding transcription factors that regulate gene expression. Paired Related Homeobox 1 (PRRX1) and Paired Related Homeobox 2(PRRX2), a homeobox genes, is overexpressed in diseases and involved in tumor metastasis, impacting cancer cell properties and metastasis. In this study, our goal was to analyze the presence of PRRX1and PRRX2 in both colorectal cancer and nearby normal tissues. The expression levels of PRRX1 and PRRX2 were evaluated in 100 colorectal tumor tissues and 100 adjacent control tissues using the Quantitative Real-Time PCR (qRT-PCR) method. Additionally, we assessed the diagnostic effectiveness of PRRX1 and PRRX2 by creating a receiver operating characteristic (ROC) curve. Our findings showed that the expression of PRRX1 and PRRX2 were significantly overexpress in colorectal cancer patients compared to the adjacent control group sample. Examination of clinicopathological characteristics of patients revealed varied correlations between PRRX1 and PRRX2 genes expressions and TMN stage (p<0.0001, p<0.0001). Also, the expression levels of PRRX1, PRRX2 between patients with LVI+ and those with LVI-, with p-values of p<0.0001, p<0.0001 for each. These findings suggest that PRRX1and PRRX2 levels could be used as possible diagnostic indicators for colorectal cancer.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 1","pages":"47-52"},"PeriodicalIF":0.0,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146184127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel peptide cocktail containing B and T cell epitopes confers robust protection against Staphylococcus aureus bacteremia in a murine model. 一种含有B和T细胞表位的新型肽鸡尾酒在小鼠模型中对金黄色葡萄球菌菌血症具有强大的保护作用。

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2026-02-13 DOI: 10.14715/cmb/2025.72.1.2

Shymaa Kamal, Samar Solyman, Amro Hanora

引用次数: 0

In-silico evaluation of the efficacy of essential oils from Artemisia absinthium, Artemisia herba-alba, and Artemisia annua against SARS-CoV-2. 苦艾、白花蒿和黄花蒿精油抗SARS-CoV-2疗效的计算机模拟评价

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2026-02-13 DOI: 10.14715/cmb/2025.72.1.3

Nawel Bouzerea, Nesrine Feknous, Sandra Feknous, Mahieddine Boumendjel, Omar Sekiou, Saada Karmi, Mohamed Anis Rouabhia, Youcef Hadef, Hassiba Rebani, Safa Abdssmad, Carmine Negro, Andrea Luvisi, Luigi De Bellis, Mourad Bouaziz, Faiza Taibi

{"title":"In-silico evaluation of the efficacy of essential oils from Artemisia absinthium, Artemisia herba-alba, and Artemisia annua against SARS-CoV-2.","authors":"Nawel Bouzerea, Nesrine Feknous, Sandra Feknous, Mahieddine Boumendjel, Omar Sekiou, Saada Karmi, Mohamed Anis Rouabhia, Youcef Hadef, Hassiba Rebani, Safa Abdssmad, Carmine Negro, Andrea Luvisi, Luigi De Bellis, Mourad Bouaziz, Faiza Taibi","doi":"10.14715/cmb/2025.72.1.3","DOIUrl":"10.14715/cmb/2025.72.1.3","url":null,"abstract":"Since the outbreak of COVID-19 in China in December 2019, the pandemic has spread to almost every country in the world, resulting in more than half a billion sick patients and more than seven million victims. The objective of our present study is to explore some plant resources available in Algeria and to verify their potential use against SARS-CoV-2, involved in COVID-19. The study used plant species belonging to the genus Artemisia and growing naturally in Algeria: Artemisia herba-alba and Artemisia absinthium. The essential oils of these two species, analysed by GC-MS, gave a fairly rich chromatographic profile. The comparison of this profile was made with a Malagasy species: Artemisia annua. An in-silico study by molecular docking was conducted on the active molecules of the three species in order to predict their efficacy and possible toxicity. The target proteins of the molecular docking are three proteases: 6LU7, 6WUU, 7JRN and a receptor linked to ACE2: 6M0J. Our study shows that out of 55 molecules tested, 8 present in these 3 plants have a very strong potential to inhibit the proteins studied: Cubebene, Elemene, Mustakone, Azulol, Myrtenyl acetate, β-Selinene, Epi-α-Cadinol, and Germacrene D. We also notice that out of the 8 molecules selected, those extracted from Artemisia absinthium are the ones with the highest scores. This plant may therefore have a very high antiviral potential. Mixtures of the three plants could be complementary in their effectiveness. These findings suggest that the selected compounds warrant further experimental validation in vitro and in vivo.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 1","pages":"16-23"},"PeriodicalIF":0.0,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146184139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Xamoterol administration into the shell nucleus accumbens of rats on anxiety-like behavior and emotional memory. 沙莫特罗对大鼠壳核伏隔核焦虑样行为和情绪记忆的影响。

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2026-02-13 DOI: 10.14715/cmb/2025.72.1.1

Kimia Najafi, Yasaman Vakiloroaya, Batool Ghorbani Yekta, Shahrzad Khakpour, Mohadeseh Ghalandari-Shamami

{"title":"Effect of Xamoterol administration into the shell nucleus accumbens of rats on anxiety-like behavior and emotional memory.","authors":"Kimia Najafi, Yasaman Vakiloroaya, Batool Ghorbani Yekta, Shahrzad Khakpour, Mohadeseh Ghalandari-Shamami","doi":"10.14715/cmb/2025.72.1.1","DOIUrl":"10.14715/cmb/2025.72.1.1","url":null,"abstract":"Beta-adrenergic receptors are involved in anxiety disorders and help regulate anxiety-like behaviors. The nucleus accumbens (NAc), an important brain structure for emotional regulation and learning, plays a crucial role in anxiety. However, limited research has addressed the specific contribution of the NAc - particularly its noradrenergic mechanisms - in anxiety. This study aimed to investigate the effects of administering different doses of Xamoterol, a partial β1-adrenergic agonist, directly into the NAc shell, to understand its impact on anxiety-related processes and the retrieval of emotional memory. Forty adult male Wistar rats were bilaterally implanted with cannulas targeting the NAc shell. The rats received intra-accumbal Xamoterol at doses of 0.01, 0.1, or 1 μg/1 μl (saline). Five minutes after drug administration, anxiety-like behavior was tested using the Elevated Plus Maze (EPM). Twenty-four hours later, an EPM retention test was conducted to assess emotional memory retrieval. In addition, an open-field test was used to measure spontaneous locomotor activity. One-way ANOVA results showed that intra-accumbal Xamoterol (0.1 and 1 μg) significantly decreased anxiety-like behavior on the EPM. Conversely, head-dipping behavior (a risk assessment measure) was significantly increased at 0.01 and 0.1 μg doses of Xamoterol, which can be interpreted as an anxiolytic effect. Furthermore, higher doses of Xamoterol (0.1 and 1 μg) led to better emotional memory performance compared to the control group. None of the Xamoterol doses produced any significant change in locomotor activity (open-field test). These results suggest that β1-adrenergic receptors in the NAc shell play a role in mediating anxiolytic behavior and enhancing emotional memory retrieval in adult male rats.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146184158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Folic acid-decorated tamoxifen-loaded covalent organic framework induces apoptosis in MCF-7 breast cancer cells via BAX/Caspase-8 upregulation. 叶酸修饰的负载他莫昔芬的共价有机框架通过上调BAX/Caspase-8诱导MCF-7乳腺癌细胞凋亡。

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2026-02-13 DOI: 10.14715/cmb/2025.72.1.4

Akash Chandran, Sudhina S, Radhika A, Muthunayagam Vibin, Janeesh Plakkal Ayyappan

{"title":"Folic acid-decorated tamoxifen-loaded covalent organic framework induces apoptosis in MCF-7 breast cancer cells via BAX/Caspase-8 upregulation.","authors":"Akash Chandran, Sudhina S, Radhika A, Muthunayagam Vibin, Janeesh Plakkal Ayyappan","doi":"10.14715/cmb/2025.72.1.4","DOIUrl":"10.14715/cmb/2025.72.1.4","url":null,"abstract":"The side effects and non-specific targeting of healthy organs associated with conventional cancer therapies necessitate the design and fabrication of novel nanomaterial-based drug delivery systems. In this study, we successfully fabricated a novel two-dimensional (2D) covalent organic framework (COF) nanomaterial. This COF was loaded with tamoxifen (TMX) and surface-modified with folic acid (FA) to achieve effective targeting for breast cancer therapy. The resulting construct, TMX@COF-FA, was characterized using PXRD, FTIR, ¹³C NMR, CHNS analysis, BET surface area analysis, TGA, UV-Vis spectroscopy, SEM, and EDX analysis. The COF carrier demonstrated a high drug encapsulation efficiency of approximately 82%. Furthermore, it exhibited a pH-responsive drug release profile, with a significantly higher release rate at pH 5.4 compared to pH 7.4, which is ideal for the acidic tumor microenvironment. Cell viability studies revealed that TMX@COF-FA induced about 90% cell death in MCF-7 breast cancer cells, while showing minimal cytotoxicity in L929 fibroblast cells. The mechanism of cell death was investigated using AO/EtBr dual staining, ROS detection assays, flow cytometry (FACS), and qRT-PCR. Collectively, our findings demonstrate that the FA-conjugated COF can efficiently deliver TMX to MCF-7 cells via folate receptor-mediated endocytosis, leading to potent cancer cell destruction. This study underscores the potential of functionalized COFs as promising targeted drug delivery platforms for breast cancer treatment.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 1","pages":"24-32"},"PeriodicalIF":0.0,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146184136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0