Cellular and molecular biology (Noisy-le-Grand, France)最新文献

{"title":"Gene polymorphism and serum level of RANKL among patients with rheumatoid arthritis: a systematic review and meta-analysis.","authors":"Pouria Salajegheh, Amirali Salajegheh, Farzaneh Yazdi","doi":"10.14715/cmb/2025.72.3.4","DOIUrl":"10.14715/cmb/2025.72.3.4","url":null,"abstract":"<p><p>Receptor activator of nuclear factor-κB ligand (RANKL) is a key mediator linking inflammation to osteoclast activation and bone erosion in rheumatoid arthritis (RA). Evidence on circulating RANKL levels and RANKL (TNFSF11) polymorphisms in RA has been inconsistent. Web of Science, Scopus, and PubMed were searched from inception to December 2025 for clinical studies comparing patients with RA and healthy controls and reporting either serum/plasma RANKL concentrations and/or RANKL genotype/allele distributions. Mean differences (MD) were pooled for circulating RANKL, and odds ratios (OR) were pooled for polymorphisms under allelic, dominant, and recessive models. Ten studies (908 RA; 525 controls) were included for serum RANKL. Serum RANKL was higher in RA than controls (pooled MD 8.16, 95% CI 3.90-12.41). For genetic analyses, three SNPs were eligible for pooling. rs9533156 showed a significant association with RA in the allelic (C vs T: OR 0.82, 95% CI 0.70-0.97) and dominant models (CC+CT vs TT: OR 0.77, 95% CI 0.61-0.98). rs2277438 showed a borderline allelic association (OR 1.21, 95% CI 1.00-1.45) and model-dependent results for the recessive contrast with heterogeneity. RA is associated with increased circulating RANKL, while genetic associations appear SNP-specific, with the most consistent evidence observed for rs9533156. Larger, multi-ethnic studies with standardized biomarker measurements are needed.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 3","pages":"28-35"},"PeriodicalIF":0.0,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148298785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated density functional theory, molecular docking, and pharmacokinetic modeling of mukonal and its derivatives as inhibitors of the Plasmodium falciparum TFIIH complex (Tfb5 subunit).","authors":"Mohammad Azhar Kamal, Nawal Helmi, Mahjabeen Rahmani, Ghufrana Abdussami, Imran Mohammad, Mohiuddin Khan Warsi, Mohammed F Aldawsari","doi":"10.14715/cmb/2025.72.3.7","DOIUrl":"https://doi.org/10.14715/cmb/2025.72.3.7","url":null,"abstract":"<p><p>Plasmodium falciparum is the most dangerous malaria parasite affecting humans. Compounds such as mukonal, 7-hydroxymukonal, and 7-methoxymukonal have shown potential for the treatment of this disease. Density Functional Theory (DFT), molecular docking, and pharmacokinetic analyses are valuable computational approaches for predicting potential drug candidates against P. falciparum. In this study, three compounds mukonal, 7-hydroxymukonal, and 7-methoxymukonal were optimized and evaluated through molecular docking and pharmacokinetic analysis against the target protein PF3D7_1441900 (transcription factor TFIIH complex subunit Tfb5, putative) from P. falciparum isolate 3D7. Frontier molecular orbital and density of states analyses revealed that 7-methoxymukonal has a relatively smaller energy gap compared with the other two compounds. In addition, 7-methoxymukonal exhibited the strongest binding affinity toward the target protein. The ADME results indicated favorable drug-like properties and low toxicity, further supporting the therapeutic potential of these compounds. Overall, our theoretical findings suggest that compound 3, namely 7-methoxymukonal, is the most promising candidate against the selected protein, in agreement with the other computed parameters.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 3","pages":"54-62"},"PeriodicalIF":0.0,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148298771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of small-molecule inhibitors targeting HER2 for breast cancer therapy using structure-based virtual screening, ADMET profiling, and molecular dynamics simulations.","authors":"Hanadi M Baeissa, Israa Jameel Hakeem","doi":"10.14715/cmb/2025.72.3.6","DOIUrl":"https://doi.org/10.14715/cmb/2025.72.3.6","url":null,"abstract":"<p><p>Human epidermal growth factor receptor 2 (HER2) is one of the key drivers of oncogenesis in HER2+ breast cancer. The overexpression resulted in uncontrollable cell division and additional tumor growth. Despite the current clinical therapeutics such as Tucatinib, Lapatinib, and Neratinib, the demand for novel inhibitors still exists. The research aimed to employ a multi-step computational framework for the discovery of highly effective small molecule inhibitors. The crystal structure of HER2 (PDB: 3PPO) was retrieved, and subsequently, virtual screening of small molecules was carried forward with the Mcule library. The screening resulted in filtering 100 compounds, out of which 45 were found to be non-toxic and had good ADME properties. BOILED-egg analysis predicted favorable HIA and BBB permeability properties surpassing the performance of clinically approved HER2 inhibitors. The docking studies singled out 11 compounds that had a high affinity towards the protein. On filtering those, the top two compounds achieved the best binding energies of -9.9 kcal/mol and -9.8 kcal/mol, respectively, outperforming the reference inhibitors. Moving ahead, 100 ns MD simulations confirmed enhanced stability, lower RMSF values, higher compactness, better solvation free energy, and persistent hydrogen bonding throughout the simulation period, showing the comparable results to the inhibitor. In comparison, findings reveal that Compound 1 hits structurally and energetically outperforming the existing therapeutics. Compound 2 showed a little less impact than compound 1, but can be considered as well as surpassing all the parameters successfully. Thereby, making the potential lead candidates for further experimental validation in breast cancer therapy.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 3","pages":"45-53"},"PeriodicalIF":0.0,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148298745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of peniocerol on human meniscal fibrochondrocyte inflammation induced by IL-1β and TNF-α through the NF-κB signaling pathway.","authors":"Brandon Eduardo Galicia-Canales, María Del Rocío Aguilar-Gaytán, Marco Antonio Álvarez-Pérez, Katia Jarquín-Yáñez, Beatriz Del Carmen Couder-Garcia, Mariano Martínez-Vázquez, Alexandra Berenice Luna-Angulo, Laura Sánchez-Chapul, Olivia Hernández-González, Monica Adelaida Saldaña-García, David Hernández-Velasco, Carlos Landa-Solís","doi":"10.14715/cmb/2025.72.3.5","DOIUrl":"https://doi.org/10.14715/cmb/2025.72.3.5","url":null,"abstract":"<p><p>It is currently known that meniscal damage has a bidirectional relationship with the onset/development of osteoarthritis (OA), making the development of new therapeutic strategies that comprehensively address both issues crucial. In this context, the present study aimed to evaluate the biological effect of peniocerol (PEN), an emerging phytopharmaceutical, in an in vitro model of inflammation induced by IL-1β and TNF-α in human meniscal fibrochondrocyte cells. Human meniscal fibrochondrocyte cells were isolated, cultured, and treated with different concentrations of PEN to determine those that promote cell proliferation and the IC₅₀ using the MTT assay. The cells were then stimulated with TNF-α (10 ng/mL) or IL-1β (5 ng/mL), with or without PEN (300 and 500 µM). Cell morphology and the expression of ACAN, type I+III collagen, type II collagen, MMP13, and p65 were evaluated by immunofluorescence. Treatment with TNF-α and IL-1β significantly reduced the expression of ACAN and type II collagen, while increasing MMP13 and p65, indicating a degenerative phenotype. Co-treatment with PEN at 300 μM showed partial improvements, while PEN at 500 µM normalized the levels of ACAN, Col2A1, and p65 to values similar to the control, while significantly inhibiting the markers for COL I+III and MMP3. In an in vitro degenerative fibrochondrocyte model, PEN at 500 μM demonstrated its ability to halt extracellular matrix degradation and actively promote its recovery, showing an optimal balance between efficacy and safety as a potential therapeutic strategy for the comprehensive treatment of OA progression.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 3","pages":"36-44"},"PeriodicalIF":0.0,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148298766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective and antioxidant effects of Opuntia ficus-indica cladodes hydroethanolic extract on castor oil-induced diarrhea in rats.","authors":"Wafa Ferjani, Nawres Zgaren, Pham My-Chan Dang, Ahmed Kouki, Mossadok Ben-Attia, Jamel El Benna, Abdelaziz Souli","doi":"10.14715/cmb/2025.72.2.4","DOIUrl":"https://doi.org/10.14715/cmb/2025.72.2.4","url":null,"abstract":"<p><p>Gastrointestinal disorders, which significantly impact human health, are a major focus of research. Diarrhea is one such disorder, affecting individuals across all age groups. Conventional anti-diarrheal drugs are often characterized byside effects, so safer alternative treatments are needed. In this context, we investigated the effects of Opuntia ficus-indica, a plant known for its therapeutic properties, by evaluating the impact of its cladode hydroethanolic extraction castor oil-induced diarrhea in rat. The hydroethanolic cladodes extract (HECE) was prepared from fresh cladodes, and its bioactive compounds were identified using high-performance liquid chromatography (HPLC). The antidiarrheal potential of HECE was evaluated at increasing doses (100, 200, 400 and 600 mg/Kg) using the castor oil-induced diarrhea model, as well as intestinal transit and enteropooling assays in rats. Histological changes in tissues were assessed using a high-performance slide scanner (Zeiss Axioscan7), and biochemical analyses were conducted on gastric and intestinal tissue homogenates. HPLC analysis identified eight phenolic compounds known to have beneficial effects on gastrointestinal disorders. In vivo, HECE administration reduced the number of stools and wet stools, normalized the antioxidant enzymes activities (Superoxide dismutase and Catalase) and levels of mediators such as plasma calcium and free iron. Histological analysis further confirmed mucosal protection, showing preserved villi and reduced epithelial disruption in treated rats. These findings suggest that HECE from Opuntia ficus-indica exhibits potent antioxidant and anti-diarrheal properties in rats, supporting its potential as a natural therapeutic agent for diarrhea.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 2","pages":"21-29"},"PeriodicalIF":0.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148298762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of calpain 1 and calpain 2 activity by dopamine in rat brain regions.","authors":"Nina Pestereva, Dmitrii Traktirov, Viktoria Burdinskaya, Ekaterina Kulikova, Timur Akbarov, Zamira Muruzheva, Marina Karpenko","doi":"10.14715/cmb/2025.72.2.3","DOIUrl":"https://doi.org/10.14715/cmb/2025.72.2.3","url":null,"abstract":"<p><p>Calpains are involved in regulating various brain functions. Limited proteolysis regulates cell proliferation, apoptosis, synaptic plasticity, long-term potentiation, and cell signaling. These are only some of the processes regulated by calpains. The current study reveals previously unexplored states of calpain activation associated with disorders of the dopaminergic system, including both hyperfunctional and hypofunctional states. In addition, activation of calpain 1 and calpain 2 affects the functioning of the central nervous system differently. Thus, this work aimed to study the effect of dopamine deficiency or excess on the activity of calpain 1 and calpain 2 in various parts of the central nervous system. We used L-dopa administration and DAT-KO rats to model a hyperdopaminergic state, and AMPT and reserpine to model a hypodopaminergic state. Dopamine deficiency causes: increased activity of calpain 1 in the striatum after AMPT administration; decreased activity of calpain 2 in the hippocampus after L-dopa administration. Excess dopamine causes: increased activity of calpains 1 and 2 in the striatum after L-dopa administration; decreased activity of calpains 1 and 2 in the midbrain after reserpine administration. Our data show that DAT knockout is associated with increased dopamine levels and increased activity of calpain 2 in the hippocampus, as well as decreased dopamine levels in the striatum and in the midbrain, accompanied by increased calpain 1 activity in the striatum.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 2","pages":"14-20"},"PeriodicalIF":0.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148298747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The paradox of telomeric chromatin state in the regulation of alternative lengthening of telomeres.","authors":"Mengjie Xiong, Wenxian Zheng, Tongxin Jia, Kailong Hou, Jiawei Liu, Runzhi Liu, Jing Liu, Kun Wang, Ruoyu Zhou, Shuting Jia","doi":"10.14715/cmb/2025.72.2.8","DOIUrl":"https://doi.org/10.14715/cmb/2025.72.2.8","url":null,"abstract":"<p><p>Telomeres are protected by the shelterin complex and consist of TTAGGG repeats. Their gradual erosion triggers replicative senescence unless counteracted by telomerase or the telomerase-independent Alternative Lengthening of Telomeres (ALT) pathway, utilized by 10-15% of cancers. Accumulating evidence indicates that the decision to activate ALT is intimately linked to the plasticity of telomeric chromatin. This review integrates recent data showing that both constitutive and facultative heterochromatin marks shape ALT activity. We summarize the dynamic regulation of telomeric chromatin and explore the contrasting evidence for two models: the 'open telomeric chromatin model,' where loss of constitutive heterochromatin (e.g., reduced H3K9me3) promotes ALT by increasing chromatin accessibility for homologous recombination (HR) factors, and the 'closed telomeric chromatin model,' where a specific gain of heterochromatic features (e.g., H3K9me3 or H3K27me3) facilitates ALT by creating a specialized phase-separated environment that promotes telomere clustering and break-induced replication (BIR). Resolving this paradox is crucial for understanding ALT initiation and for developing promising synthetic-lethal strategies against ALT-dependent cancers.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 2","pages":"61-71"},"PeriodicalIF":0.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148298736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glutathione S-Transferase Mu1 polymorphisms and environmental factors in cervical cancer susceptibility: a systematic review and meta-analysis.","authors":"Teega-Wendé Clarisse Ouedraogo, Bapio Valérie Elvira Jean Télesphore Bazie, Abibou Simporé, Abdoul Karim Ouattara, Rogomenoma Alice Ouedraogo, Lassina Traoré, Tempoubila Edwige Yelemkouré, Yves Donald Kagembega, Wendémi Alexis Sama, Mousso Savadogo, Tani Sagna, Abdou Azaque Zouré, Theodora Mahoukèdè Zohoncon, Florencia Wendkuuni Djigma, Damnoti Simplice Karou, Jacques Simporé","doi":"10.14715/cmb/2025.72.2.5","DOIUrl":"https://doi.org/10.14715/cmb/2025.72.2.5","url":null,"abstract":"<p><p>Cervical cancer is a multifactorial disease like any other human cancer. Among these factors, genetic polymorphisms of Glutathione S-Transferase Mu1 (GSTM1) have been incriminated, but the studies results remain controversial. The objective of this meta-analysis was to study the influence of GSTM1 polymorphisms on the occurrence of cervical cancer and to explore interactions between genes and human papillomavirus (HPV) infection and exposure to tobacco smoke. A meta-analysis was conducted on studies published up to June 12, 2025, from six databases: ScienceDirect, Embase, Scopus, PubMed, Google Scholar, Web of Science. Eligible studies included all case-control investigations that assessed the association between GSTM1 polymorphisms and the risk of cervical cancer. Odds ratios and confidence intervals from the studies were used to estimate the combined effect size. Statistical analyses were conducted using both the DerSimonian and Laird random-effects model and Mantel and Haenszel fixed-effect model with a 95% confidence interval. Subgroup analyses were performed to identify potential sources of variability. A significant association was observed between the GSTM1-null and an increased risk of Cervical Cancer (ORCC=1.47, Pz=0.011) and Squamous Intraepithelial Lesions (ORSIL=1.42, Pz=0.035) compared with the GSTM1-present. The included studies showed high heterogeneity. Overall, carriers of the GSTM1-null had a higher likelihood of developing Cervical Cancer compared to carriers of the GSTM1-present. In subgroup analyses, an increased risk associated with the GSTM1-null was found among Asian populations (ORCC=1.54, Pz=0.002) and according to sample type, in blood DNA extracts (ORCC=1.27, Pz=0.052). Furthermore, the GSTM1-null was associated with increased risk in HPV+ women (ORCC=4.88, Pz=0.005) and in women exposed to tobacco smoke (ORCC=1.35, Pz=0.033). According to histological type, GSTM1-null was also associated with the development of Squamous Cell Carcinoma (ORSCC=1.52, Pz=0.020). in conclusion, GSTM1-nullwere associated with an increased risk of cervical cancer in women, especially in HPV+ women and those exposed to tobacco smoke.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 2","pages":"30-43"},"PeriodicalIF":0.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148298720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The neutrophil-to-lymphocyte ratio as an inflammatory marker in preeclampsia: a retrospective cohort study from Saudi Arabia.","authors":"Ayed A Dera","doi":"10.14715/cmb/2025.72.2.6","DOIUrl":"https://doi.org/10.14715/cmb/2025.72.2.6","url":null,"abstract":"<p><p>Preeclampsia (PE) is a major hypertensive disorder of pregnancy, with systemic inflammation being a key component of its pathogenesis. The neutrophil-to-lymphocyte ratio (NLR) has been investigated as an inflammatory biomarker for PE; however, its levels are rarely compared directly to both healthy pregnant and non-pregnant controls. This study aimed to evaluate the role of NLR in PE by comparing its levels across these three distinct groups. This retrospective study analyzed medical records from Abha Maternity and Children's Hospital (2022-2023), including 236 PE cases, 260 healthy pregnancies, and 100 non-pregnant controls. Biochemical and hematological parameters were assessed, as well as Receiver Operating Characteristic (ROC) analysis, which evaluated NLR's discriminatory power. Women with PE exhibited significantly elevated NLR compared to both the HP and NP groups (p < 0.001). ROC analysis showed that NLR could distinguish NP from PE with an AUC of 0.643 (p<0.0001) and NP from HP with an AUC of 0.623 (p=0.0003). An elevated NLR was associated with a 40.7% increased relative risk (RR=1.41, p<0.0001) and nearly threefold higher odds (OR=3.08, p<0.0001) of developing PE. It is concluded that NLR is significantly elevated in PE and demonstrates a significant association with increased risk of the disorder. These findings support its potential utility as an accessible, cost-effective inflammatory biomarker for aiding in the risk stratification of pregnant women. Further prospective studies are warranted to validate its clinical diagnostic value.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 2","pages":"44-51"},"PeriodicalIF":0.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148298765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of citalopram treatment on memory performance, mood state, catalase, BDNF, and MDA in REM sleep-deprived rats.","authors":"Elham Mahdavi, Batool Ghorbani Yekta, Fereshteh Atabi","doi":"10.14715/cmb/2025.72.2.7","DOIUrl":"https://doi.org/10.14715/cmb/2025.72.2.7","url":null,"abstract":"<p><p>Rapid-eye movement (REM) sleep is a critical stage of sleep that plays a pivotal role in memory formation, consolidation, and mood regulation. While REM sleep deprivation (REM SD) has been shown to impair cognitive and affective functions, findings across studies remain inconsistent. REM SD may also influence the expression of brain-derived neurotrophic factor (BDNF), a key protein involved in memory and mood-related processes. In this study, we investigated the effects of REM SD (6 h/day for 7 days) on behavioral and cognitive performance, BDNF levels in the prefrontal cortex, and serum markers of oxidative stress-catalase (CAT, an antioxidant enzyme) and malondialdehyde (MDA, a lipid peroxidation product). Additionally, we evaluated the potential therapeutic effect of citalopram (20 mg/kg, i.p., for 7 days) on REM SD-induced behavioral deficits. Our results demonstrated that REM SD increased locomotor activity and rearing behavior, impaired passive avoidance memory, and reduced pain threshold. REM SD also downregulated prefrontal BDNF expression but did not alter serum CAT or MDA levels. Citalopram treatment reversed REM SD-induced hyperlocomotion, increased rearing, pain hypersensitivity, and BDNF downregulation; however, it did not significantly improve memory impairment. Depressive-like behavior remained unchanged across groups. These findings suggest that BDNF modulation may underlie both the detrimental effects of REM SD and the therapeutic actions of citalopram, whereas peripheral oxidative stress markers (CAT and MDA) do not appear to play a major role in this paradigm.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"72 2","pages":"52-60"},"PeriodicalIF":0.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148298739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}