Cellular and molecular biology (Noisy-le-Grand, France)最新文献

{"title":"Epigenetic and genetic events of oral squamous cell carcinoma: perspective on DNA methylation, silencing of tumor suppressor gene, and activating oncogenes.","authors":"Zainab Nizar Jawad","doi":"10.14715/cmb/2025.71.9.12","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.9.12","url":null,"abstract":"<p><p>Oral squamous cell carcinoma (OSCC) is a major health burden in most parts of the world, and pathogenesis of the disease is strongly associated with a complex combination between genetic mutations and epigenetic alterations. The current study will explore the DNA methylation phenomenon and how it affects the silencing of tumour suppressor genes and activate oncogenes in OSCC in a bid to explain the driving molecular process behind tumour development. We evaluated 50 samples of the OSCC tissues with 50 of the adjacent normal tissues, evaluated DNA methylation patterns with methylation-specific PCR and quantitative reverse-transcriptase PCR measured the expression of the DNA repair genes like BRCA1 and MLH1, among others. This finding showed different levels of DNA methylation of cancerous and normal tissues with hypermethylation causing the inactivation of important tumor suppressor genes and hypomethylation causing the activation of oncogenes. In addition, the downregulation of DNA repair genes was noted to be highly significant in OSCC samples indicating that genomic-instability may be related to epigenetic changes. These results demonstrate that aberrant DNA methylation is central to OSCC growth and progression, thus helping in the future use of methylation patterns that can be used as early detection, diagnosis, and prognostic biomarkers. Our findings support the idea that genetic, along with epigenetic, profiling is an issue of key importance toward comprehending OSCC biology and personalized therapeutic interventions. It is recommended that further confirmation be carried out to verify the clinical significance of such epigenetic markers in bigger cohorts, as well as testing such markers in guided therapies, which might eventually lead to better patient outcomes in the management of oral cancer.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 9","pages":"96-104"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of ovarian aging markers and hormonal regulation in menopausal transition.","authors":"Samah Amer Hammood, Noor Alamer, Mohammed Kareem S ALquraish, Mohauman M Majeed","doi":"10.14715/cmb/2025.71.9.15","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.9.15","url":null,"abstract":"<p><p>Menopause and ovarian dysfunction are consequences of ovarian aging, a continuous and natural process in women. Early symptoms of ovarian aging include inadequate response to ovarian stimulation, irregular menstruation, and loss of follicular function, which collectively contribute to a decline in fertility as women age. The effects of aging on the ovaries are more pronounced than on other organs, with deterioration in the follicular pool and oocyte quality influenced by endocrine, genetic, and metabolic factors. This study aimed to examine the correlation between menopausal symptoms and serum levels of vitamin D, anti-Müllerian hormone (AMH), and other fertility-related hormones, as well as their impact on the timing of menopause. Ninety-two female participants were recruited from the Fertility Center Laboratories and AL-Saader Medical City in Al-Najaf province. A structured questionnaire assessing age, weight, height, and the severity of eleven menopausal symptoms-rated on a scale from 0 (absent) to 4 (extremely severe)-was administered. Symptoms evaluated included hot flushes, heart discomfort, sleep disturbances, muscle and joint pain, psychological issues, and urogenital problems. Serum levels of follicle-stimulating hormone (FSH), 25-hydroxyvitamin D, AMH, and luteinizing hormone (LH) were measured using the ELISA method. The results showed a significant negative correlation between serum AMH and vitamin D levels with menopausal symptoms and advancing age in postmenopausal women. Vitamin D levels were also negatively correlated with age, depression scores, and body mass index (BMI). Women with vitamin D deficiency and decreased AMH experienced more severe menopausal symptoms such as hot flushes, heart discomfort, depression, irritability, bladder problems, and musculoskeletal pain. These findings suggest that low AMH and vitamin D levels during menopause, compared to younger control women aged 20-40 years, may exacerbate menopausal symptoms. Supplementation with vitamin D, AMH, and reproductive hormone regulators, alongside lifestyle management, may benefit menopausal women suffering from fertility decline and severe menopausal symptoms.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 9","pages":"117-124"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular docking study and ADMET prediction of the effects of some food alkaloids on thyroxine homeostasis through their interactions with human thyroxine-binding globulin.","authors":"Naima Maouche, Nesrine Lenchi","doi":"10.14715/cmb/2025.71.9.9","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.9.9","url":null,"abstract":"<p><p>Thyroid hormones (THs) play a vital role in several physiological functions of the body. At the circulatory level, thyroxine (T4) has been found to be the predominant form of THs. The distribution of T4 in the blood is mainly carried out by human thyroxine-binding globulin (hTBG). This process can be interfered with by various natural substances present in foods, particularly alkaloids. Some of these alkaloids have been shown to possess a cyclic chemical structure similar to that of T4. It has therefore been hypothesised that this class could potentially compete with T4 transport in the bloodstream. A molecular docking study and ADMET prediction were performed with ten selected food alkaloids. Predicted ADMET analysis revealed that all compounds tested had adequate solubility, high human gastrointestinal absorption and minimal risk of hepatotoxicity and cardiotoxicity. Molecular docking data showed that piperine, nigellidine, capsaicin, nigellicine, and 3-hydroxyquinine had a high affinity for hTBG, with respective binding energies of - 8.1, - 8.0, - 7.7, - 7.2 and - 7.1 kcal/mol. This finding indicates that these alkaloids were successfully positioned in the binding site of hTBG and had the ability to compete with T4 and increase its free level in the bloodstream. Therefore, two suggestions can be withdrawn, depending on the physiological state of the thyroid gland. Overconsumption of these alkaloids may lead to an imbalance in T4 homeostasis in both healthy individuals and hyperthyroid patients. Conversely, this competitive dynamic may offer a therapeutic advantage in the management of hypothyroidism.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 9","pages":"71-79"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of Epstein-Barr virus in invasive ductal carcinoma of the breast: a case-control study.","authors":"Mahmoud Mohammed Mahmoud, Abdulla Kamil Abdulla, Dler Omar Mohammed","doi":"10.14715/cmb/2025.71.9.13","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.9.13","url":null,"abstract":"<p><p>The uncertain contribution of Epstein-Barr virus (EBV) to the etiological processes underlying invasive ductal carcinoma (IDC) of the mammary gland, especially in relation to its molecular interactions within inflamed histological contexts, remains to be elucidated. This case-control research assessed the link between EBV infection and mammary IDC in a population of Iraqi females from Kirkuk. A total of 300 breast tissue specimens preserved in paraffin blocks were evaluated, including 150 samples diagnosed with IDC and 150 samples classified as fibroadenoma serving as controls. EBV latent membrane protein-1 expression was identified through the application of immunohistochemical staining and polymerase chain reaction methodologies. EBV positivity, defined as detection by both IHC and PCR, was observed in 7.3% of IDC cases and 4% of controls, with no statistically significant difference between groups (P=0.996). No significant association was found between EBV presence and estrogen or progesterone receptor status, while Her-2 expression differed significantly between EBV-positive and EBV-negative patients (P<0.001). EBV was more frequently detected in grade I tumors and stage II breast cancers, and older patients showed a higher prevalence of EBV infection. The results indicate that although Epstein-Barr virus (EBV) is identifiable in a fraction of invasive ductal carcinoma (IDC) breast specimens, a definitive causative relationship between EBV presence and IDC occurrence within this demographic is not established. Nonetheless, EBV detection appears to exhibit higher frequency in specific histopathological grades, clinical stages, and patient age categories.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 9","pages":"105-110"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone morphogenetic protein signaling pathway: an essential role in intestinal homeostasis and diseases.","authors":"Emma Cogo, Françoise Guéraud, Pascale Plaisancie","doi":"10.14715/cmb/2025.71.9.5","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.9.5","url":null,"abstract":"<p><p>The bone morphogenetic protein (BMP) signaling pathway is a fundamental regulator of intestinal homeostasis, orchestrating the delicate balance between stem cell proliferation and epithelial differentiation along the crypt-villus axis. In opposition to the Wnt signaling pathway, BMP signaling promotes epithelial maturation and inhibits excessive stem cell expansion, thereby ensuring proper renewal and functional integrity of the intestinal epithelium. Both epithelial and mesenchymal cell populations actively contribute to BMP signaling; mesenchymal cells serve as a primary source of BMP ligands and antagonists, while epithelial cells predominantly express BMP receptors and downstream effectors. This dynamic epithelial-mesenchymal dialogue establishes and maintains the intestinal stem cell niche and structural organization of the crypts. Dysregulation of BMP signaling has been increasingly implicated in the pathogenesis of inflammatory bowel diseases (IBD) such as Crohn's disease and ulcerative colitis, as well as in colorectal cancer (CRC). In inflammatory conditions, modulation of BMP ligands and antagonists influences epithelial regeneration and immune responses, highlighting their potential anti-inflammatory and anti-fibrotic roles. Conversely, in colorectal carcinogenesis, alterations in BMP pathway components-including mutations in BMP receptors and Smad effectors, alongside aberrant expression of BMP antagonists like Gremlin-1-disrupt the balance of intestinal homeostasis, promoting tumor initiation, progression, and metastatic potential via both canonical and non-canonical signaling mechanisms. This review comprehensively summarizes the current understanding of BMP signaling in intestinal physiology and pathology, emphasizing the critical interplay between epithelial and mesenchymal compartments, and the impact of the genetic context and pathway modulators. Further elucidation of BMP pathway dynamics promises novel therapeutic strategies for intestinal diseases through targeted modulation of this pivotal signaling cascade.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 9","pages":"38-49"},"PeriodicalIF":0.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SIRT1 modulation and lipid profile alterations in the cellular regulation of blood lipids in renal disorders among extremely obese individuals.","authors":"Zain Alabdean Azeez, Elyes Chabchoub","doi":"10.14715/cmb/2025.71.8.12","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.8.12","url":null,"abstract":"<p><p>The global epidemic of overweight and obesity is closely linked to the development of chronic kidney disease (CKD), with extremely obese individuals facing a particularly high risk. This study aimed to assess the relationship between lipid profile levels, SIRT1 expression, and RNA-34a-5P in the regulation of blood lipid levels among severely obese individuals with renal diseases. Conducted over six months in three specialized hospitals, the study included 100 participants divided into two groups: 50 obese individuals with renal diseases and 50 obese controls without renal problems. Ethical standards, including confidentiality and informed consent, were strictly observed. Biochemical assessments included measurements of total cholesterol, LDL, HDL, triglycerides, creatinine, GFR, SIRT1 protein (via Western blotting), and RNA-34a-5P expression (via qPCR). Statistical analysis was performed using SPSS v26 and Pearson's correlation. The results revealed a negative association between RNA-34a-5P expression and total cholesterol, LDL, triglycerides, and SIRT1 expression, while a positive but non-significant association was found with HDL and GFR. Notably, SIRT1 expression was significantly downregulated in the patient group compared to controls. These findings provide compelling evidence that SIRT1 expression is markedly reduced in extremely obese individuals with renal diseases, suggesting a potential molecular link between SIRT1, lipid metabolism, and renal dysfunction in severe obesity.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 8","pages":"80-88"},"PeriodicalIF":0.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An early prediction of postprandial glycemia evolution using the MD001 algorithm: a muticentre prospective trial.","authors":"Veronique Di Costanzo, Pauline Scheapelynck, Vincent Gardan, Sophie Lafond, Jean Noel Gouze","doi":"10.14715/cmb/2025.71.8.3","DOIUrl":"10.14715/cmb/2025.71.8.3","url":null,"abstract":"<p><p>To date, the closed-loop system represents the best commercialized management of type 1 diabetes. However, mealtimes still require carbohydrate estimation and are often associated with postprandial hyperglycemia which may contribute to poor metabolic control and long -term complications. A multicentre, prospective, non-interventional clinical trial was designed to determine the effectiveness of a novel algorithm to predict changes in blood glucose levels two hours after a usual meal. Forty patients were included, and 765 meals were analyzed of which 278 were followed by a postprandial hyperglycemic event i.e. value > 160 mg/dL two hours after the start of the meal. The developed algorithm correctly predicts the postprandial hyperglycemia risk or absence of risk in 87% of cases. The results suggest that early prediction of the glycemic evolution within a few minutes after the end of a meal can considerably improve the postprandial hyperglycemia management and thus reduce the associated emotional burden. The study was supported by M-DT1 SAS, France.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 8","pages":"14-21"},"PeriodicalIF":0.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, characterization, and transmissible factors of foodborne pathogens in the Al-Qassim Region, Saudi Arabia.","authors":"Abdulrahman Almujaidel, Adil Abalkhail, Ayman Elbehiry","doi":"10.14715/cmb/2025.71.8.8","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.8.8","url":null,"abstract":"<p><p>Foodborne illnesses pose a significant public health threat globally, particularly in Saudi Arabia, where the rapid growth of the food service sector has increased the risk of exposure to multidrug-resistant (MDR) bacteria. Traditional microbiological methods are often time-consuming and may lack precision, highlighting the need for faster and more accurate diagnostic alternatives. In this study, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) was employed for the rapid and precise identification of bacterial contaminants in ready-to-eat (RTE) foods, alongside an assessment of their antibiotic resistance profiles. A total of 80 RTE food samples-including chicken shawarma, shish tawook, chicken burgers, and falafel sandwiches-were collected from restaurants across the Al-Qassim region between September and November 2024. Bacterial identification was performed using standard culturing techniques in combination with MALDI-TOF MS, while antimicrobial susceptibility testing was conducted using the Kirby-Bauer disk diffusion method in accordance with CLSI guidelines. The predominant pathogens identified were Escherichia coli (E. coli) (32.5%), Staphylococcus aureus (S. aureus) (27.5%), and Acinetobacter baumannii (A. baumannii) (18.75%), with the highest contamination observed in chicken shawarma samples. MALDI-TOF MS provided high-confidence species-level identifications, with score ranges between 2.00 and 2.49. E. coli isolates exhibited complete resistance (100%) to ampicillin, amoxicillin-clavulanic acid, cefoxitin, and cephalothin. High resistance rates were also observed for norfloxacin (80.8%) and tetracycline (73.1%). Similar MDR patterns were detected in S. aureus and A. baumannii. Imipenem remained the most effective antibiotic, showing 100% susceptibility among E. coli and S. aureus isolates and 93.3% effectiveness against A. baumannii. These findings underscore the critical need for routine microbial surveillance, implementation of rapid diagnostic tools, and enforcement of stringent food safety regulations to curb the spread of antimicrobial-resistant pathogens through the food supply chain.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 8","pages":"48-59"},"PeriodicalIF":0.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of cumin and coriander seeds consumption on dyslipidemia, abdominal fat, body weight, and lipid profile in rat models.","authors":"Sehar Iqbal, Rida Fatima Saeed, Saira Zafar, Asma Saleem Qazi, Syed Hassan Bin Usman Shah, Juweria Abid, Umar Farooq, Sajeela Akram, Abdul Momin Rizwan Ahmad","doi":"10.14715/cmb/2025.71.8.9","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.8.9","url":null,"abstract":"<p><p>Dyslipidemia is considered a crucial risk factor for high risk of atherosclerosis and cardiovascular diseases. Cumin and coriander seeds are well-known flavoring agents that contain nutraceutical properties and appear to have beneficial health effects. A study was therefore conducted to investigate the effects of cumin and coriander seeds on body weight, abdominal fat and lipid profile in rats. For this purpose, 18 male albino rats were fed on fat-rich diet (diet A) for 14 days, followed by the control diet mixed separately with 20 g of cumin (diet B) and 20g of coriander (diet C) for the next 4 weeks. The body weights and lipid profiles of the rats were analyzed at the start of the intervention and thereafter weekly. Results showed that cumin seeds help to reduce body weight, ranging from 178g to 141g (37g), even for a short time. Post-mortem examination revealed that rats fed cumin diet have less fat accumulation in abdominal cavity compared to control and coriander seeds diets. Elevated values of high-density lipoprotein (HDL) were observed particularly from day 14 to 35 in rats fed with both cumin (from 72.3 to 88.0 mg/dl) and coriander seeds, (from 63.2 to 88.0 mg/dl) as compared to control group (55.0 mg/dl) (P>0.05). Also, a remarkable reduction of low-density lipoprotein (LDL) and total lipid profile was seen in cumin group, as compared to the controls. This interventional study reported that cumin seeds helped in the significant reduction of weight, abdominal fat and total lipid profile while increasing the HDL in rats.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 8","pages":"60-66"},"PeriodicalIF":0.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Donor site healing in follicular unit extraction hair transplantation: current evidence, cellular mechanisms, and future research directions.","authors":"Ney Arencibia Pérez, María José Guerrero Roldán","doi":"10.14715/cmb/2025.71.8.14","DOIUrl":"10.14715/cmb/2025.71.8.14","url":null,"abstract":"<p><p>Follicular unit extraction (FUE) has become a leading technique in hair transplantation, yet optimal management of the donor area remains a clinical challenge. This systematic review analyzes intraoperative and postoperative interventions applied to the donor area in FUE hair transplantation, with a focus on both clinical outcomes and the cellular and molecular mechanisms involved in tissue repair, inflammatory response, and regenerative processes. A comprehensive literature search was conducted in PubMed and EMBASE (January 2000-June 2025), identifying clinical studies that evaluated donor area treatments and reported outcomes related to healing, inflammation, infection, and patient satisfaction. Four studies met the inclusion criteria, encompassing corticosteroid infiltration, platelet-rich plasma (PRP), timing of postoperative care, and hair follicle-derived microtissue (HFMT) application. Evidence suggests that intraoperative corticosteroid use significantly reduces postoperative edema, likely by modulating local inflammatory pathways and vascular permeability. Early postoperative wound care is associated with decreased folliculitis incidence, highlighting the importance of timely intervention in preventing microbial colonization and dysregulated immune responses. While PRP and HFMT show potential for enhancing cellular proliferation and accelerating wound closure, current data are limited by heterogeneity in study design and lack of standardized molecular endpoints. The review identifies a critical gap in mechanistic studies exploring the cellular dynamics of donor area healing, including the roles of keratinocytes, fibroblasts, and immune cells. Future research integrating molecular biomarkers and advanced imaging is needed to elucidate the pathways driving optimal tissue regeneration. These insights may inform evidence-based protocols that not only improve clinical outcomes but also advance our understanding of scalp wound biology in the context of FUE.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 8","pages":"93-98"},"PeriodicalIF":0.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}