Expression analysis of C-FOS and XRCC3 Thr241Met polymorphism in gastric cancer.

Abstract

Gastric cancer is one of the causes of deaths related to cancer across the globe and both genetic and environmental factors are the most prominent. Causes of its pathogenesis. This paper researches the expression of the C-FOS gene. and Thr241Met talking in the XRCC3 gene in patients with gastric cancer and healthy individuals. Controls, in an attempt to clarify their behavior as possible disease susceptibility molecular markers. A total of 100 gastric cancer patients and 100 matched healthy individuals were enrolled, with genomic DNA and RNA extracted from blood samples. Quantitative real-time PCR was used to assess C-FOS expression, while PCR-RFLP determined XRCC3 Thr241Met genotypes. The C-FOS and the Thr/Met XRCC3 genotypes, 12 genotypes revealed that C-FOS was significantly overexpressed in patients than in controls (P <0.001). The XRCC3 Thr/Met genotype was very frequent in patients, as well (P =0.0020). Also, blood type A, family history of gastric cancer, and residing in the country were shown to be categorized as major factors of the risk, and were not significant factors. These results indicate that upregulation of C-FOS and the XRCC3 Thr241Met variant are risk factors of gastric cancer and that blood type and familial history are additional risk factors. We present in our findings that molecular profiling coupled with demographic profiling is highly relevant in risk assessment and early detection techniques in gastric cancer. The study contributes to the further comprehension of the molecular pathogenesis of gastric carcinogenesis and suggests C-FOS and XRCC3 as possible clinical and epidemiological markers.