Cellular and molecular biology (Noisy-le-Grand, France)最新文献_第5页

The use of monoclonal antibodies for the treatment of atherosclerosis: current status and prospects. 单克隆抗体在动脉粥样硬化治疗中的应用：现状与展望。

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2025-07-30 DOI: 10.14715/cmb/2025.71.7.9

Alexander V Blagov, Elizaveta M Pleshko, Olga N Maltseva, Alikhan Z Asoyan, Alessio L Ravani, Alexander N Orekhov

{"title":"The use of monoclonal antibodies for the treatment of atherosclerosis: current status and prospects.","authors":"Alexander V Blagov, Elizaveta M Pleshko, Olga N Maltseva, Alikhan Z Asoyan, Alessio L Ravani, Alexander N Orekhov","doi":"10.14715/cmb/2025.71.7.9","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.9","url":null,"abstract":"Atherosclerosis remains a leading cause of cardiovascular morbidity and mortality worldwide, underlying major conditions such as coronary heart disease and stroke. The pathogenesis of atherosclerosis is tightly linked to chronic inflammation and dysregulated lipid metabolism, processes that are also implicated in other inflammatory diseases like rheumatoid arthritis and psoriasis. Monoclonal antibodies (mAbs) have emerged as a promising therapeutic strategy, offering targeted intervention against key molecular drivers of atherosclerosis. This review summarizes recent advances in the development and clinical application of mAbs targeting both lipid-lowering pathways-such as low-density lipoprotein (LDL) and proprotein convertase subtilisin/kexin type 9 (PCSK9)-and inflammatory mediators, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-17 (IL-17). Notably, anti-PCSK9 antibodies like alirocumab and evolocumab have demonstrated significant reductions in LDL-C levels and cardiovascular events in large-scale clinical trials. Similarly, antibodies targeting inflammatory cytokines have shown efficacy in reducing vascular inflammation and associated risks. The review also discusses the advantages and limitations of therapeutic mAbs, such as their high specificity, potential for adverse immune responses, and challenges related to tissue penetration and cost. Overall, monoclonal antibody therapy represents a significant advancement in the management of atherosclerosis, with ongoing research aimed at optimizing efficacy, safety, and accessibility. Future directions include the development of novel mAbs and combination therapies to further improve cardiovascular outcomes in patients with atherosclerotic disease.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"64-71"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-inflammatory effect of thyme on rheumatoid arthritis in animal model. 百里香对类风湿关节炎动物模型的抗炎作用。

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2025-07-30 DOI: 10.14715/cmb/2025.71.7.8

Sana Seamand Sabir Blbas, Hiwa Ramadhan Fatah, Safia Sabr Ibrahim Blbas

{"title":"Anti-inflammatory effect of thyme on rheumatoid arthritis in animal model.","authors":"Sana Seamand Sabir Blbas, Hiwa Ramadhan Fatah, Safia Sabr Ibrahim Blbas","doi":"10.14715/cmb/2025.71.7.8","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.8","url":null,"abstract":"Thyme (Thymus vulgaris) is a Mediterranean herb known for its culinary, cosmetic, and medicinal applications as it has been discovered that the plant has many clinical properties such as anti-inflammatory, anti-microbial, and antioxidant properties. Sandwich Elisa technique was used to determine the concentration of cytokines. Phenolic contents and other active compounds of thyme were analyzed by Gas Chromatography-Mass Spectrometry (GC-Mass). In this study 15 male adult albino rats were divided into 3 groups (n=5), group one (G1) was the control group which fed on basal diet. Group two (G2) was the Rheumatoid arthritis (RA) group in which the rats were inoculated with 0.1 ml of CFA (Complete Freund's Adjuvant) yet fed on basal diet. Group three (G3) was the treatment group in which rats were inoculated with CFA along with the administration of thyme extract orally for 22 days. The results show that treatment with thyme extract significantly reduced proinflammatory cytokines IL-1 (interleukin-1), IL-6 (interleukin-6) and TNF- α (tumor-necrosis factor-alpha). Anti-inflammatory IL-10 (interleukin-10) showed a significant increase in the thyme-treated group. CD4 T (cluster of differentiation-4) cell levels showed a significant difference, while sCD14 (soluble cluster of differentiation-14) levels were non-significant in the thyme group compared to the RA group. Inflammatory markers (C-reactive proteins) CRP and (anti-cyclic citrullinated peptide) Anti-CCP antibodies were both significantly elevated in RA and significantly reduced by thyme treatment. Although body weight changes were statistically non-significant, they were visibly prominent. Paw edema was significantly decreased in the thyme-treated group. (Matrix-metalloproteinase) MMP-1 levels and neutrophil counts were both elevated in RA and significantly reduced following thyme extract treatment.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"56-63"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of abiotic factors on Zingiber officinale and Glycyrrhiza glabra to extract bioactive compounds under different time incubation and different salt concentrations. 不同培养时间、不同盐浓度下非生物因子对鲜姜、甘草提取活性物质的影响。

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2025-07-30 DOI: 10.14715/cmb/2025.71.7.7

Mustafa I Almaghasla, Tahir Khan, Niama Kanwal, Jameel M Al-Khayri

{"title":"Effects of abiotic factors on Zingiber officinale and Glycyrrhiza glabra to extract bioactive compounds under different time incubation and different salt concentrations.","authors":"Mustafa I Almaghasla, Tahir Khan, Niama Kanwal, Jameel M Al-Khayri","doi":"10.14715/cmb/2025.71.7.7","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.7","url":null,"abstract":"Zingiber officinale, commonly known as ginger, and Glycyrrhiza glabra, commonly known as licorice, are medicinal plants that are rich in bioactive compounds with various health benefits. This study aimed to investigate the effects of different growth durations and salt concentrations on the production of bioactive compounds by these plants. This experiment was conducted under natural conditions and the plants were subjected to salt stress at different stages of growth. This analysis focused on assessing the production of polysaccharides, flavonoids, ergosterol, adenine, adenosine, hypoxanthine, and guanosine by UV spectrophotometer and high-performance liquid chromatography (HPLC) in both plants. The results showed that Z. officinale exhibited the highest polysaccharide content at 20-d of growth with 3mM salt, whereas G. glabra showed slightly lower polysaccharide content. Similarly, Z. officinale had higher flavonoid content at 25-d of growth with 5 mM salt compared to G. glabra. Additionally, Z. officinale demonstrated higher concentrations of ergosterol, adenine, adenosine, hypoxanthine, and guanosine than G. glabra, particularly at 25-d of growth with 5 mM salt. This study provides valuable insights into the production of bioactive compounds in Z. officinale and G. glabra under different growth conditions, which can be beneficial for optimizing their cultivation and utilization in various applications including pharmaceuticals and functional foods.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"49-55"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of smoking and environmental toxins on diabetic retinopathy: role of trace elements, lipid profiles, and vitamin A. 吸烟和环境毒素对糖尿病视网膜病变的影响：微量元素、脂质谱和维生素A的作用。

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2025-07-30 DOI: 10.14715/cmb/2025.71.7.13

Nagham Hadi Alrikabi, Hajer Chemingui, Soheir N Abd El-Rahman, Madiha Kamoun

{"title":"Impact of smoking and environmental toxins on diabetic retinopathy: role of trace elements, lipid profiles, and vitamin A.","authors":"Nagham Hadi Alrikabi, Hajer Chemingui, Soheir N Abd El-Rahman, Madiha Kamoun","doi":"10.14715/cmb/2025.71.7.13","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.13","url":null,"abstract":"Diabetic retinopathy (DR), a leading cause of adult blindness, is influenced by physiological factors such as lipid profiles, vitamin A, and trace elements, as well as environmental factors like smoking. This study investigated the relationship between HbA1C, glucose levels, trace elements (lead, zinc, selenium, magnesium), vitamin A, and lipid profiles (TC, TG, HDL, LDL, VLDL) in type 2 diabetes (T2D) and DR patients, considering the impact of smoking. The study, conducted at Nasiriya General Hospital and the Center for Endocrinology and Diabetes (May 2023-March 2024), included three age groups (15-35, 35-55, and 55-75 years) divided into smokers and non-smokers. Results showed significantly higher glucose, HbA1C, lipid and selenium levels in T2D and DR patients compared to controls (p≤0.05), with smokers exhibiting greater lead levels. Zinc and vitamin A were significantly lower in DR patients, particularly among smokers. The findings highlight smoking as a source of lead and other toxins that exacerbate DR and diabetes-related complications, emphasizing the critical link between environmental health and chronic disease management.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"92-101"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low frequency of HER2 expression in colorectal cancer: A Tunisian single-center study. HER2在结直肠癌中的低频率表达：突尼斯单中心研究

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2025-07-30 DOI: 10.14715/cmb/2025.71.7.6

Khouloud Ben Lazreg, Wiem Majdoub, Ahlem Bdioui, Marwa Krifa, Zaineb Lajmi, Oussema Belkacem, Mariem Alaya, Sarra Mestiri, Sihem Hmissa, Nabiha Missaoui

{"title":"Low frequency of HER2 expression in colorectal cancer: A Tunisian single-center study.","authors":"Khouloud Ben Lazreg, Wiem Majdoub, Ahlem Bdioui, Marwa Krifa, Zaineb Lajmi, Oussema Belkacem, Mariem Alaya, Sarra Mestiri, Sihem Hmissa, Nabiha Missaoui","doi":"10.14715/cmb/2025.71.7.6","DOIUrl":"https://doi.org/10.14715/cmb/2025.71.7.6","url":null,"abstract":"HER2 expression is a potential theranostic and prognostic marker in some cancers, particularly in breast and gastric cancers. However, published data on HER2 expression in colorectal cancer (CRC) remain controversial. This study investigates the immunohistochemical and molecular expression of HER2 in primary CRC and evaluates its clinicopathological and prognostic significance in Tunisian patients. A retrospective analysis was conducted on 144 CRC patients. HER2 status was assessed by immunohistochemistry and tissue microarray analysis, following the diagnostic criteria for gastroesophageal adenocarcinoma. CRC cases with ambiguous results underwent chromogenic in situ hybridization. The mean patient age was 61.9 years (male-to-female ratio: 1.18:1). Tumors were classified as colonic (74.3%) or rectal (25.7%), with 45.8% located in the left colon. Stage III disease was identified in 37.5% of cases, and distant metastases were present in 13.9%. HER2 expression results were as follows: negative (score 0/1+) in 142 cases (98.6%), equivocal (score 2+) in one case (0.7%), and overexpressed (score 3+) in one case (0.7%). No HER2 gene amplification was detected, and none of the metastatic CRC cases showed HER2 immunostaining. These findings suggest that HER2 overexpression and amplification in CRC are rarer than previously reported, highlighting the need for multicenter Tunisian studies to validate these results. The variability in HER2 immunostaining criteria further underscores the importance of a standardized scoring system to ensure consistency in both diagnosis and research.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":"71 7","pages":"39-48"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influences of Total Alkaloids in Caulis Mahoniae on Proliferation and Apoptosis of Cervical Cancer Cells and the Caspase-3 Expression. 麻藤总生物碱对宫颈癌细胞增殖、凋亡及Caspase-3表达的影响

IF 1.6

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2022-06-30 DOI: 10.14715/cmb/2022.68.6.26

Guoyue Yan, Man Zhang, Ke Lu, Jiyong Lin, Ning Jiang, Bingbing Zhao, Dan Liang, Gang Fang

{"title":"Influences of Total Alkaloids in Caulis Mahoniae on Proliferation and Apoptosis of Cervical Cancer Cells and the Caspase-3 Expression.","authors":"Guoyue Yan, Man Zhang, Ke Lu, Jiyong Lin, Ning Jiang, Bingbing Zhao, Dan Liang, Gang Fang","doi":"10.14715/cmb/2022.68.6.26","DOIUrl":"https://doi.org/10.14715/cmb/2022.68.6.26","url":null,"abstract":"The study aimed to investigate the influences of the active ingredient in Caulis Mahoniae, total alkaloids, on the proliferation and apoptosis of cervical cancer cells and the caspase-3 expression. The total alkaloids were extracted in vitro from Caulis Mahoniae, and cervical cancer HeLa cell lines were used as experimental objects. The half inhibitory concentration (IC50) of total alkaloids on HeLa cell lines was detected via the preliminary experiment, the influences of total alkaloids at different concentrations on the proliferation of HeLa cell lines were detected via methyl thiazolyl tetrazolium (MTT) assay, and the cell growth curve was plotted. Moreover, the cell cycle and apoptosis after treatment with total alkaloids at different concentrations were detected via flow cytometry, and the caspase-3 gene and protein expressions were detected via reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. The IC50 of total alkaloids in Caulis Mahoniaeon HeLa cell lines was 12.5 μg/mL. With the gradual increase of concentration of total alkaloids in the treatment of cervical cancer cells, the inhibitory rate on cancer cells was gradually increased, and the proportion of cells in the G0/G1 phase was gradually decreased, while that in S and G2/M phases was gradually increased. Besides, with the increase in the concentration of total alkaloids, the apoptotic rate of cervical cancer cells was gradually increased, and both caspase-3 gene and protein expressions were also gradually increased. The total alkaloids extracted from Caulis Mahoniae can effectively inhibit the proliferation and promote the apoptosis of cervical cancer HeLa cells, which may be realized by promoting the expression of apoptosis-related factor caspase-3.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":" ","pages":"161-166"},"PeriodicalIF":1.6,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33529233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of drug resistance genes of integrons in clinical isolates of Escherichia coli from elderly bloodstream infections. 老年血流感染临床分离大肠埃希菌整合子耐药基因分析。

IF 1.6

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2022-06-30 DOI: 10.14715/cmb/2022.68.6.11

Wei Liu, Fangjian Zhao, Jian Chen

{"title":"Analysis of drug resistance genes of integrons in clinical isolates of Escherichia coli from elderly bloodstream infections.","authors":"Wei Liu, Fangjian Zhao, Jian Chen","doi":"10.14715/cmb/2022.68.6.11","DOIUrl":"https://doi.org/10.14715/cmb/2022.68.6.11","url":null,"abstract":"This experiment was carried out to provide a basis for the treatment of clinical bloodstream infections by analyzing the drug resistance characteristics and integrated gene distribution of Escherichia coli in bloodstream infections in elderly patients. For this aim, E. coli were collected for bacterial identification and drug sensitivity testing from bloodstream infections in elderly patients in the hospital from January 2016 to December 2019. ESBLs positive strains were assayed for genotypes and their integron carriage rates by PCR amplification. The characteristics and differences of various genotype rates were compared and analyzed. Results showed that a total of 230 E. coli strains were isolated. The detection rate of ESBLs-producing bacteria was 37.39 %. ESBLs-producing E. coli showed a high rate of resistance to cefepime, levofloxacin, cotrimoxazole, and ticarcillin/clavulanic acid (>40%). The resistance rate of 230 strains of E. coli to meropenem, minocycline, amikacin, gentamicin and cefoxitin was less than 20%. Among the ESBLs-producing E. coli in bloodstream infections in elderly patients, CTX-M-9 accounted for 27.91%, CTX-M-2 for 17.44%, and SHV for 13.95%. The detection rate of type I integrated genes was 41.30%, and type II and III integrated genes were not detected. ESBLs-producing genotyping-positive bacteria were detected with more than 50% of type I integrated genes. It was concluded that type I integrated genes in ESBLs-producing E. coli isolated from elderly patients carried resistance genes such as CTX-M-9 and CTX-M-2 aggravating multi-drug resistance in bacteria.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":" ","pages":"67-72"},"PeriodicalIF":1.6,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33504965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Circulating cell-free-DNA concentration is a good biomarker for diagnosis of colorectal cancer in Mexican patients. 循环游离细胞dna浓度是诊断墨西哥结直肠癌的良好生物标志物。

IF 1.6

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2022-06-30 DOI: 10.14715/cmb/2022.68.6.1

Anilú Margarita Saucedo-Sariñana, Carlos Roberto Lugo-Escalante, Patricio Barros-Núñez, María Eugenia Marín-Contreras, Tomas Daniel Pineda-Razo, Ignacio Mariscal-Ramírez, Martha Patricia Gallegos-Arreola, Mónica Alejandra Rosales-Reynoso

{"title":"Circulating cell-free-DNA concentration is a good biomarker for diagnosis of colorectal cancer in Mexican patients.","authors":"Anilú Margarita Saucedo-Sariñana, Carlos Roberto Lugo-Escalante, Patricio Barros-Núñez, María Eugenia Marín-Contreras, Tomas Daniel Pineda-Razo, Ignacio Mariscal-Ramírez, Martha Patricia Gallegos-Arreola, Mónica Alejandra Rosales-Reynoso","doi":"10.14715/cmb/2022.68.6.1","DOIUrl":"https://doi.org/10.14715/cmb/2022.68.6.1","url":null,"abstract":"Colorectal cancer (CRC) is the third most common cancer in the world. Overall survival is related to clinical stage: more advanced stages show lower survival rates; therefore, they need to be monitored regularly with new, less invasive and more specific biomarkers. The concentration and integrity index of circulating cell-free DNA (ccfDNA) have been proposed as potential diagnostic and prognostic biomarkers for CRC, however, inconsistent results are still observed in different reports. Here we analyze these potential CRC biomarkers in a Mexican population. In this study, 124 patients with sporadic CRC and 37 healthy individuals were examined as a reference group. The ccfDNA was isolated from plasma samples of all included subjects. The ccfDNA concentration was determined by fluorometry and the integrity index (ALU247/ALU115 ratio) by quantitative PCR amplification (qPCR) of ALU sequences. The results show that ccfDNA concentration was higher in CRC patients than in the reference group (P=0.001). The integrity index showed no significant differences between these groups (P=0.258), except for histological type (P=0.012). A higher ccfDNA concentration was also associated with patients younger than 50 years (P=0.030). The ccfDNA concentration showed significant discriminatory power (AUC: 0.854, C.I.: 0.78-0.92, P=0.001) between patients and the reference group and between tumor-node-metastasis (TNM) stages. In conclusion, ccfDNA concentration proves to be a good diagnostic biomarker for CRC patients, whereas the integrity index did not show diagnostic utility.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":" ","pages":"1-8"},"PeriodicalIF":1.6,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33505413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Pseudoangiosarcomatous squamous cell carcinoma: a rare subtype of squamous cell carcinoma that needs to be differentiated from angiosarcoma and has a poor prognosis. 假性血管肉瘤性鳞状细胞癌:一种罕见的鳞状细胞癌亚型，需与血管肉瘤鉴别，预后较差。

IF 1.6

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2022-06-30 DOI: 10.14715/cmb/2022.68.6.10

Yuan Zhan, Hongping Wan, Liqing Wu, Xian Ge, Xinhua Xie, Luoyan Wu, Yong Cai

{"title":"Pseudoangiosarcomatous squamous cell carcinoma: a rare subtype of squamous cell carcinoma that needs to be differentiated from angiosarcoma and has a poor prognosis.","authors":"Yuan Zhan, Hongping Wan, Liqing Wu, Xian Ge, Xinhua Xie, Luoyan Wu, Yong Cai","doi":"10.14715/cmb/2022.68.6.10","DOIUrl":"https://doi.org/10.14715/cmb/2022.68.6.10","url":null,"abstract":"This study aimed to investigate the formation mechanism of Pseudoangiosarcoma squamous cell carcinoma (PASCC). The researchers reviewed ten cases of PASCC and summarize their clinical outcomes, pathological morphological traits, immunophenotypes, treatment plans and the corresponding follow-up data. Results showed that the pathological morphology revealed complex reticular structures, where numerous tracts of anastomose, and lacunar structures lined with atypical neoplastic cells, which resembles the histopathological appearance of angiosarcoma. Particularly, we observed pathologic patterns that resemble Sclerosing Epithelioid Fibrosarcoma (or Myxoid Fibrosarcoma) in the patients who suffered a relapse. All cases present negative results for vascular markers (CD31, ERG) and positive results for epithelial markers (CK-pan, p40). The average age of the participants is 60 years old (range: 48-79), relative aged, and there is no significant difference between male and female participants (6 men and 4 women). The locations of neoplasms involve face (n=3), upper limbs (n=1), waist(n=1), cervix uteri (n=1), lungs (n=2), thyroid (n=1), and breasts (n=1). All participants had received clinical follow-ups that range from 4 to 47 months, during which the researchers observed Lymph Node Metastases developed in three participants (out of 10; 30%); Distant Metastases in five participants (out of 10; 50%); two local recurrences at the site of surgical resection; and four deaths due to disease (out of 10; 40%), with 9.5 months estimated median survival time and 9 months mean survival time. It was concluded that PASCC presents the tendency for recurrence and metastasis. Accurate pathological diagnosis and standardized medical procedures are crucial to the treatment of PASCC. Epithelial-Mesenchymal Transformation (EMT) and P53 gene mutation are involved in the formation of PASCC.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":" ","pages":"62-66"},"PeriodicalIF":1.6,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33504967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The mechanism of lncRNA TALNEC2 regulating miR-19a-3p/JNK to alleviate cerebral ischemia injury in rats with acute cerebral infarction. lncRNA TALNEC2调控miR-19a-3p/JNK减轻急性脑梗死大鼠脑缺血损伤的机制

IF 1.6

Cellular and molecular biology (Noisy-le-Grand, France) Pub Date : 2022-06-30 DOI: 10.14715/cmb/2022.68.6.3

Danping Luan, Chun Jiang

{"title":"The mechanism of lncRNA TALNEC2 regulating miR-19a-3p/JNK to alleviate cerebral ischemia injury in rats with acute cerebral infarction.","authors":"Danping Luan, Chun Jiang","doi":"10.14715/cmb/2022.68.6.3","DOIUrl":"https://doi.org/10.14715/cmb/2022.68.6.3","url":null,"abstract":"Ischemia-reperfusion (I-R) is renowned as a key approach in recovery related to cerebral infarction and further promotes succeeding infarction development. This study investigated the fundamental molecular function of the TALNEC2 in the pathogenesis of cerebral infarction to provide insights on the potential novel therapeutic agents in cerebral infarction. RT-qPCR measured expression of TALNEC2 and JNK in human neural cell line SH-SY5Y. Cell transfection upregulated or silenced the genes with MTT assay examining cell viability. RT-qPCR detected cell death in the apoptosis biomarker caspase-3, inflammation in the biomarkers C-reactive protein (CRP) and IL-6 and verified cell proliferation via the ki67 and PCNA markers. Luciferase assay was performed to see the luciferase activity and western blotting determined the protein expression of JNK in proliferation, inflammation. The results demonstrated that TALNEC2 was highly expressed after OGD/R treatment in nerve cells after cerebral infarction. In addition, TALNEC2 silencing prevented apoptosis and inflammation of nerve cells after cerebral infarction. TALNEC2 directly interacted with miR-19a-3p to regulate JNK protein expression. Lastly, miR-19a-3p inhibitor abolished the protective effect of si-TALNEC2 against OGD/R induced damage in vitro. In summary, this study has demonstrated that TALNEC2 is a positive moderator for pathogenesis of cerebral infarction. Furthermore, our conclusions provide further insights on the interplay among TALNEC2, miR-19a-3p and JNK in cerebral infarction. It has demonstrated herein that TALNEC2 positively modulates JNK post-transcriptional expression through miR-19a-3p sponging in cerebral Infarction offering a novel therapy target for cerebral infarction.","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":" ","pages":"17-24"},"PeriodicalIF":1.6,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33505411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0