Chromatographia最新文献

{"title":"Computer-Assisted Modeling and Simulation of a Dielectrophoresis-based Microseparator for Blood Cells Separation Applications","authors":"Elnaz Poorreza","doi":"10.1007/s10337-025-04385-9","DOIUrl":"10.1007/s10337-025-04385-9","url":null,"abstract":"<div><p>The objective of the present numerical investigation is to propose a microseparator specifically engineered for the separation of platelets from white blood cells (WBCs). This microfluidic device, which incorporates circular electrodes operating at a minimal voltage of 1.4 V, and a frequency of 100 kHz, is recommended for the targeted separation of platelets and WBCs utilizing dielectrophoresis (DEP) force. The utilization of a low-voltage level ensures the preservation of the viability of biological cells, a fundamental consideration in medical applications. Simulation results are presented to illustrate the electric potential, electric field, velocity, pressure, and DEP force profiles concerning two distinct particles. Through a comparative analysis employing the finite element method, we delineate the implications of modulating the inlet velocity field on the pressure exerted upon the particles. Subsequently, the impact of varying the fluid conductivity and input voltage on electrodes within the microchannel on particle separation was examined. It is anticipated that this comprehensive design is exceptionally conducive to the realization of DEP-based practical biochips for cell separation.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"88 3","pages":"225 - 242"},"PeriodicalIF":1.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An RP-HPLC Method for the Simultaneous Analysis of Selected Antiviral Drugs","authors":"Mehmet Emre Özdemirhan,&nbsp;Gürkan Özen,&nbsp;Emirhan Nemutlu","doi":"10.1007/s10337-025-04386-8","DOIUrl":"10.1007/s10337-025-04386-8","url":null,"abstract":"<div><p>A rapid and efficient reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the simultaneous determination of six antiviral drugs: emtricitabine (EMT), entecavir (ENT), favipiravir (FAV), ganciclovir (GAN), valaciclovir (VAL), and valganciclovir (VLG). Chromatographic separation was achieved on an ExsilTM Mono 100 C18 column using a mobile phase of 50 mM phosphate buffer (pH 4): acetonitrile (90:10, v/v) at a flow rate of 0.5 mL/min. UV detection was performed at 215 nm. The method was validated according to ICH Q2(R2) guidelines, demonstrating excellent linearity (<i>R</i>² &gt; 0.9938) across the concentration ranges of 1.0–150 μg/mL for EMT, ENT and VLG, and 5.0–200 μg/mL for FAV, GAN and VAL. The LOD and LOQ values ranged from 0.02 to 0.48 μg/mL and 0.05 to 1.45 μg/mL, respectively. Intra-day and inter-day precision, expressed as relative standard deviation (RSD), were less than 1.82%, while accuracy, expressed as relative error (RE), was within ± 1.34%. Recovery studies yielded values ranging from 98.12% to 101.60%, further confirming method accuracy. The method proved to be selective, robust, and suitable for the quantitative analysis of these antiviral drugs in pharmaceutical formulations, with results obtained for commercially available tablets demonstrating excellent agreement with labeled contents. Notably, stability studies revealed the susceptibility of EMT, ENT, and FAV to degradation under refrigerated storage, highlighting the need for timely analysis of these compounds. In addition, the greenness, blueness, and whiteness of the method were evaluated, and the developed method performs strongly across environmental (AGREE score: 0.79), practical (BAGI index: 85), and sustainability (whiteness value: 74.7) criteria, reinforcing its viability as a sustainable approach for antiviral analysis. This validated RP-HPLC method provides a reliable and efficient tool for the quality control of these essential antiviral medications.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"88 3","pages":"179 - 191"},"PeriodicalIF":1.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromatographic Separation and Trace Level Quantification of Ten Nitrosamine Genotoxic Impurities in a Single Chromatography Technique through Atmospheric Pressure Chemical Ionization (APCI) Coupled with Triple Quardrapole Analyser in Telmisartan Drug Products","authors":"Mehul Pathak,&nbsp;Dhara D. Patel,&nbsp;Dalip Kumar","doi":"10.1007/s10337-025-04387-7","DOIUrl":"10.1007/s10337-025-04387-7","url":null,"abstract":"<div><p>The detection and quantification of genotoxic impurities, specifically nitrosamines, in pharmaceuticals have garnered high regulatory attention due to their potential carcinogenic effects. This study presents a comprehensive validation of a liquid chromatography with Atmospheric Pressure Chemical Ionization (APCI) Coupled with Triple Quardrapole Analyser (LC-MS/MS) method tailored for the determination of multiple nitrosamine impurities including N-nitrosodimethylamine (NDMA), N-nitroso methyl butyl amine (NMBA), N-nitrosodiethylamine (NDEA), N-nitroso diethyl isopropyl amine (NEIPA), N-nitrosodiisopropylamine (NDIPA), N-nitroso methyl ethyl amine (NMPA), N-nitroso propyl amine (NDPA), N-nitrosopiperidine (NPIP), N-nitrosopyrrolidine (NPYR), and N-nitroso butyl amine (NDBA) in telmisartan tablet formulations. The method was meticulously validated according to the International Council for Harmonisation (ICH) guidelines, focusing on critical parameters such as specificity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), and filter compatibility. The specificity of the method was rigorously established, demonstrating unequivocal differentiation between nitrosamine impurities and potential interferences from excipients and active pharmaceutical ingredients (APIs). Recovery studies validated the method’s accuracy, yielding recoveries within the acceptable range of 70–120% across various concentration levels, confirming its reliability for routine analysis. Precision was assessed through the relative standard deviation (RSD) of multiple replicate analyses, with RSD values consistently below the ICH threshold of &lt; 15%, underscoring the method’s reproducibility. Sensitivity assessments revealed exceptional LODs as low as 10.76 ppb and LOQs around 33.00 ppb for several nitrosamines, significantly exceeding current regulatory limits for genotoxic impurities. Evaluations of filter compatibility indicated that both 0.22 µm PVDF and 0.2 µm nylon filters are effective for sample preparation, ensuring the integrity of the analytes during the filtration process. Upon application of the validated method to commercial 40 mg telmisartan tablets, all targeted nitrosamines were undetected, affirming the method’s applicability in routine quality control settings. This work culminates in the establishment of a highly sensitive, specific, and robust LC–MS/MS methodology for the detection of nitrosamine impurities in telmisartan, addressing regulatory concerns while ensuring patient safety. The low detection thresholds provided by this method position it as an invaluable tool for pharmaceutical manufacturers and regulatory bodies, facilitating stringent monitoring and compliance with safety standards, ultimately enhancing the safety profile of medicinal products in the market.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"88 2","pages":"169 - 177"},"PeriodicalIF":1.2,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of Confirmatory Quantitative and Screening Methods for 63 Prohibited Substances in Horse Urine Using LC–MS/MS","authors":"Erol Kabil,&nbsp;Eylem Funda Göktaş","doi":"10.1007/s10337-024-04384-2","DOIUrl":"10.1007/s10337-024-04384-2","url":null,"abstract":"<div><p>The number of potentially abused substances in horse racing is very large and it is not easy to analyse the full range. To facilitate the analyses of substances of abuse, a method has been developed for 63 substances in horse urine using liquid chromatography–tandem mass spectrometry. The developed method has been validated according to the 2021/808/EC and AORC criteria. The horse urine samples were extracted after enzymatic hydrolysis via a C8-BCX SPE cartridge with an automated SPE and analyzed by LC–MS/MS. According to the validation study results, the within-laboratory reproducibility CV was no more than 13.9% and the average recoveries ranged from 90.71 to 117.94% for repeatability and reproducibility. The decision limits (CCα) for quantitative analysis and detection capabilities (CCβ) for qualitative screening were close to the targeted limit for each substance. The results of the present study showed that the method was able to perform quantitative analyses of 54 substances and screening analyses of 9 substances (total 63) in horse urine and that the results were valid and applicable. The validated method was successfully applied to five proficiency tests and three suspected samples.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"88 2","pages":"151 - 168"},"PeriodicalIF":1.2,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Short-Chain Organic Acids, Cations and Anions on the Retention of Citicoline Under Hydrophilic Interaction Liquid Chromatography Conditions","authors":"Sofiane Derbouz,&nbsp;Moulay-Hassane Guermouche,&nbsp;Saliha Guermouche,&nbsp;Ouassila Ferroukhi","doi":"10.1007/s10337-024-04383-3","DOIUrl":"10.1007/s10337-024-04383-3","url":null,"abstract":"<div><p>The optimization of mobile phase composition in HPLC is crucial for achieving excellent chromatographic performance. Mobile phase additives are very often added to control retention, resolution, peak shape and efficiency. According to the Hofmeister series, additives are classified into chaotropic and kosmotropic agents. When ionizable compounds are analysed by hydrophilic interaction liquid chromatography (HILIC), additives can control the electrostatic interactions and affect the chromatographic parameters. In this study, citicoline, a neurotransmitter, was analysed under HILIC conditions using various additives. Due to the solubility limit in high organic content, the composition of the mobile phase was fixed at a 2/1 (V/V) acetonitrile/water ratio, and the salt concentration set to 25 mM. A total of 21 additives were tested, including short-chain organic acids (formic, acetic, propionic, trifluoroacetic and trichloroacetic acid), cations (lithium, sodium, potassium and ammonium) and anions (acetate, bromide, chloride, dihydrogen citrate, dihydrogen phosphate, nitrate, perchlorate and tetrafluoroborate). With additive-free mobile phases, weak retention of citicoline was observed, which can be explained by the small thickness of the water layer on the surface of the silica stationary phase and electrostatic repulsion between deprotonated silanols and negatively charged citicoline. However, the use of additives improves retention. Short-chain organic acids increased retention, but produce poor peak shape. Cations affected retention in the following order: <span>({{varvec{L}}{varvec{i}}}^{+}&lt;{{varvec{N}}{varvec{H}}}_{4}^{+}&lt;{{varvec{N}}{varvec{a}}}^{+}&lt;{{varvec{K}}}^{+})</span>, corresponding to the reversed Hofmeister series. The anions trend was: <span>({{varvec{C}}{varvec{H}}}_{3}{varvec{C}}{{varvec{O}}{varvec{O}}}^{-}&lt;{{varvec{H}}}_{2}{{varvec{P}}{varvec{O}}}_{4}^{-}&lt;{{varvec{B}}{varvec{r}}}^{-}&lt;{{varvec{C}}{varvec{l}}}^{-}&lt;{{varvec{H}}}_{2}{{varvec{C}}{varvec{i}}{varvec{t}}{varvec{r}}{varvec{a}}{varvec{t}}{varvec{e}}}^{-}&lt;{{varvec{N}}{varvec{O}}}_{3}^{-}&lt;{{varvec{C}}{varvec{l}}{varvec{O}}}_{4}^{-}&lt;{{varvec{B}}{varvec{F}}}_{4}^{-})</span>, which corresponds to the direct Hofmeister series, except for acetate and dihydrogen phosphate.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"88 2","pages":"107 - 116"},"PeriodicalIF":1.2,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly Statistical Chemometric-Assisted Spectrophotometric and Chromatographic Model Development in Hydrotropic Solutions for Commercial Drug Formulations","authors":"Pritam S. Jain,&nbsp;Deepak M. Patil","doi":"10.1007/s10337-024-04381-5","DOIUrl":"10.1007/s10337-024-04381-5","url":null,"abstract":"<div><p>Oflox-TZ, Dto, and Ofnida syrup are well-known antimicrobial combination medications used to treat illnesses caused by parasites and bacteria. Environmentally sustainable model creation for antimicrobial compounds utilizing chemometric application, isosbestic point, and dual wavelength assisted by spectrophotometric and chromatographic analysis using ANOVA comparison with all developed methods. Deconvolution of the spectral overlaps and harmful use of organic solvents in spectrophotometric and chromatographic conditions with these combinations were performed using the partial least squares method, either with or without variable selection methods such as principal component regression. A good agreement was found when comparing the findings of the developed methods statistically to an ANOVA, indicating the efficacy of the suggested methodology. Calibration and validation sets comprising 24 and 12 samples, respectively, were carefully developed using partially factorial designs for the experiments at different dosages. The developed models were validated according to established International Council for Harmonisation (ICH) strategies. For the dual-wavelength approach, Beer’s plot confirmed linearity between 2 and 12 µg/mL for (OFL) and 5 and 30 µg/mL for Tinidazole (TZ) respectively. The mean percent recoveries were found to 101.0% and 102.0% for OFL and TZ. Chemometrics-assisted UV spectrophotometry, employing both partial least squares (PLS) and principal component regression (PCR) analysis models, yielded mean OFL recoveries of 102.3% (PLS) and 102.4% (PCR), and TZ recoveries of 102.6% (PLS) and 102.6% (PCR). Additionally, chromatographic model development using hydrotropic solutions yielded mean percentage recoveries of 100.2% and 100.6% for OFL and TZ, respectively, in commercial drug formulations. The study found that the proposed chemometric-assisted spectrophotometric and chromatographic methods could reliably determine OFL and TZ concentrations in both laboratory-prepared mixtures and pharmaceutical preparations, thereby proving them to be useful analytical instruments for quality control and in-process testing of different pharmaceutical drug preparations. This approach provides a significant outcome over the use of less polluted solvents, making it more precise and economical.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"88 2","pages":"139 - 149"},"PeriodicalIF":1.2,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enantioseparations of the Six Basic Drugs by MIL-100(Fe)-Modified Open-Tubular Columns in Capillary Electrochromatography","authors":"Xiaozhen Shao,&nbsp;Guangfu Xu,&nbsp;Jiaquan Chen,&nbsp;Pandeng Miao,&nbsp;Ke Yang,&nbsp;Yingxiang Du,&nbsp;Tao Yu","doi":"10.1007/s10337-024-04382-4","DOIUrl":"10.1007/s10337-024-04382-4","url":null,"abstract":"<div><p>Metal–organic frameworks (MOFs) have a wide range of applications in separation and analytical sciences due to their unique structures. MIL-100(Fe) was immobilized on the inner wall of the capillary column based on the immobilized cysteine (Cys)-triggered in situ growth (ICISG) strategy. By applying this column to capillary electrophoresis, a novel enantioselective separation system based on MIL-100(Fe) nanomaterial was established. By utilizing lactobionic acid (LA) as the chiral selector and optimizing the experimental conditions such as buffer pH, LA concentration and the methanol addition ratio, the CEC system demonstrated a significantly enhanced enantioseparation ability for six basic drugs. The modified capillary column was characterized by scanning electron microscope (SEM), energy-dispersive X-ray spectroscope (EDS) and X-ray diffractometer. The experimental results showed that MIL-100(Fe) was successfully grown on the inner wall of the capillary column. In the present study, the repeatability of the coated column was investigated and the relative standard deviations (RSDs) of the resolution and the migration time for intra-day, inter-day and inter-column were within 8%, proving the good repeatability of the coated column.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"88 2","pages":"117 - 125"},"PeriodicalIF":1.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Use of Weakly Basic Ion Exchange Resin Amberlite IRA-68 for the Chromatographic Separation of Rare Earth Elements","authors":"Zbigniew Samczyński","doi":"10.1007/s10337-024-04380-6","DOIUrl":"10.1007/s10337-024-04380-6","url":null,"abstract":"<div><p>The possibility of applying the system: weakly basic Amberlite IRA-68 resin-nitrilotriacetic acid (NTA) solutions for the separation of rare earth elements (REE) by ion exchange chromatography was investigated. Preliminary research results revealed that the affinity of REE towards the ion exchanger is closely correlated with the stability of their negative complexes that they form with NTA. Three separate groups of lanthanides could be distinguished, i.e. light (La, Ce, Pr, Nd), medium (Y, Sm, Eu, Gd, Tb, Dy, Ho, Er) and heavy (Tm, Yb, Lu, Sc). Moreover, it seemed that within the first and third groups it was possible to individually separate elements from each other. Based on the experimentally obtained relationships, the theoretically assumed course of the ion exchange reaction of anionic REE complexes with NTA on the Amberlite IRA-68 resin was confirmed. The influence of the ion exchanger particle size, column size and composition of the mobile phase, i.e. pH, NTA and neutral salt (NaNO₃) concentration, on the chromatographic separation of REE was investigated. It has been shown that the proper selection of these parameters makes it possible not only to divide REE into the three groups mentioned above, but also to individually separate some elements, i.e. La, Ce, Pr, Nd, Tm, Yb, Lu and Sc.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"88 2","pages":"127 - 138"},"PeriodicalIF":1.2,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10337-024-04380-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Comb-Like Polyelectrolytes in Capillary Electrophoresis","authors":"Aleksandra A. Adamova,&nbsp;Mikhail S. Orlov,&nbsp;Nadezhda S. Rakovskaya,&nbsp;Petr A. Fetin,&nbsp;Lyudmila A. Kartsova","doi":"10.1007/s10337-024-04376-2","DOIUrl":"10.1007/s10337-024-04376-2","url":null,"abstract":"<div><p>New comb-like polyelectrolytes with triethylammonium (pAUTEA-Br), N-methyl-piperidinium (pAUMP-Br), N-methyl-morpholinium (pAUMM-Br) and pyridinium (pAUPy-Br) cation groups were first used to separate steroid hormones, biogenic amines and amino acids under capillary electrophoresis conditions. The polymers acted as modifiers of electrophoretic systems and were used as dynamic coatings of inner surface of fused-silica capillary and additives to background electrolyte to perform capillary electrochromatography and micellar electrokinetic chromatography regimes, leading to increase in separation selectivity and efficiency, and as reagents for indirect spectrophotometric detection of amino acids. New materials also showed activity during on-line preconcentration of biogenic amines and reduced their limits of detections by 10 times. Thus, new comb-like polyelectrolytes were demonstrated to act as multifunctional materials for capillary electrophoresis. This study discusses the features of the influence of the polyelectrolytes on electrophoretic separation capabilities and their possibilities in separation of model biologically active substances. Modifiers of this type significantly expand the analytical capabilities of the capillary electrophoresis method.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"88 1","pages":"33 - 43"},"PeriodicalIF":1.2,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143110174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPTLC Based Marker and Fingerprint Analysis Coupled with Multivariate Analysis of Different Parts of Cyanthillium cinereum from Different Geographical Locations","authors":"Jyoti Dahiya,&nbsp;Anupam K. Mangal,&nbsp;Rajesh Bolleddu,&nbsp;Deepak Kumar,&nbsp;Salik Abdullah,&nbsp;Shyam Baboo Prasad,&nbsp;Sreya Dutta,&nbsp;Simmi Mall,&nbsp;Kalyan Hazra,&nbsp;G. Babu","doi":"10.1007/s10337-024-04379-z","DOIUrl":"10.1007/s10337-024-04379-z","url":null,"abstract":"<div><p>A rapid rise in population has led to the exploitation of natural resources, especially medicinal plants to meet human needs. Repeated harvesting of non-renewable or slow growing plant parts can severely damage plant systems. The whole plant of <i>Cyanthillium cinereum</i> (<i>Sahadevi</i>) is the key ingredient in numerous extract-based commercial herbal formulations beneficial for skin and hair related diseases. Although, the official crude drug <i>Sahadevi</i> covers the whole plant of <i>Cyanthillium cinereum</i> but many reports focus on individual parts as well. This study conducts a systematic comparative chemical investigation using fingerprint and marker based study of different parts (root, stem, leaves and flower) collected from both low land (Indo-gangetic plain) and high altitude regions, comparing then with the whole plant to determine which portion contribute most to the plant’s chemical composition. We employed, multivariate tools including principal component analysis and hierarchical clustering analysis on the fingerprint data for similarity analysis. The results indicate a close chemical similarity between the leaf and flower samples of <i>Cyanthillium cinereum</i> with whole plant based on comparable lupeol content and chemical profile. The high similarity observed in the leaves suggests that they may be used as a substitute for the whole plant, supporting efforts to enhance the plant’s life expectancy. Additionally, this study concluded that samples collected from lowland areas contain a higher concentration of bioactive component than those from high-altitude regions.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"88 2","pages":"95 - 106"},"PeriodicalIF":1.2,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}