Chromatographia最新文献_第6页

Analytical Solutions for Characterization of the Chemical Composition Distribution of Linear Low-Density Polyethylene: Leveraging Structure Retention Relationships and Relative Energy Density 线性低密度聚乙烯化学成分分布表征的解析解：利用结构保留关系和相对能量密度

IF 1.3 4区化学

Chromatographia Pub Date : 2025-11-28 DOI: 10.1007/s10337-025-04457-w

Subrajeet Deshmukh, Jan-Hendrik Arndt, Tibor Macko, Masud Monwar, Jeff Fodor, Eric Schwerdtfeger, Robert Brüll

{"title":"Analytical Solutions for Characterization of the Chemical Composition Distribution of Linear Low-Density Polyethylene: Leveraging Structure Retention Relationships and Relative Energy Density","authors":"Subrajeet Deshmukh, Jan-Hendrik Arndt, Tibor Macko, Masud Monwar, Jeff Fodor, Eric Schwerdtfeger, Robert Brüll","doi":"10.1007/s10337-025-04457-w","DOIUrl":"10.1007/s10337-025-04457-w","url":null,"abstract":"<div><p>Ethylene/1-olefin copolymers have held industrial significance for a considerable period. A series of copolymers with identical molar mass and varying 1-hexene levels were synthesized using supported metallocene catalyst. Various analytical techniques were used to characterize the copolymers, focusing on the effect of increasing 1-hexene content on their chemical properties, specifically chemical composition distribution (CCD). Initially, the investigations carried out by differential scanning calorimetry (DSC) revealed broad and asymmetric thermograms with the presence of a distinct shoulder in samples with higher 1-hexene content. Furthermore, HT-GPC with IR5 detector revealed uniform chemical composition (1-hexene concentration) along the molar mass axis. Examination of the chemical composition distribution (CCD) was conducted using two variants of high-temperature liquid adsorption chromatography (HT-LAC), namely solvent gradient interaction chromatography (SGIC) and thermal gradient interaction chromatography (TGIC). Initial testing by employing solvents common to HT-LAC showed that the elution behavior did not correspond with the distinct thermal properties DSC. In the next step, new desorption-promoting solvents were identified using a recent approach that integrates structure retention relationships (SRR), a measure of solvent–stationary phase interactions, with Hansen solubility parameters (HSP), assessing polymer–solvent interactions. The broad and asymmetric CCD in samples with higher SCB, characterized by a main peak and a shoulder, was confirmed by SGIC using newly identified desorption-promoting solvents identified via the SRR–HSP method. This CCD profile was also corroborated by TGIC using a binary solvent mixture as the mobile phase.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"89 1","pages":"79 - 89"},"PeriodicalIF":1.3,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10337-025-04457-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145983239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Assay for Isoprenaline HCl in a Pharmaceutical Injectable Formulation by RP-HPLC Method: A Molecular Docking and DFT Study of Isoprenaline HCl 反相高效液相色谱法测定注射制剂中盐酸异丙肾上腺素的含量：盐酸异丙肾上腺素的分子对接和DFT研究

IF 1.3 4区化学

Chromatographia Pub Date : 2025-11-27 DOI: 10.1007/s10337-025-04456-x

Deniz Ceyhan, Pelin Koseoglu-Yilmaz, Yelda Yalcin Gurkan, Ayca Karasakal

{"title":"An Assay for Isoprenaline HCl in a Pharmaceutical Injectable Formulation by RP-HPLC Method: A Molecular Docking and DFT Study of Isoprenaline HCl","authors":"Deniz Ceyhan, Pelin Koseoglu-Yilmaz, Yelda Yalcin Gurkan, Ayca Karasakal","doi":"10.1007/s10337-025-04456-x","DOIUrl":"10.1007/s10337-025-04456-x","url":null,"abstract":"<div><p>Isoprenaline (IPN) HCl is a nonselective β‐adrenergic agonist used in treating heart block and low heart rate. To determine the right percentage of isoprenaline HCl in a pharmaceutical the injectable solution, an RP-HPLC method was designed in the scope of this study. Chromatographic separation was carried out on an Inertsil C18 column (150 × 4.6 mm, 2.7 µm) at 40 °C using methanol/0.1% o-phosphoric acid (v/v) (5:95) as the mobile phase at a flow rate of 1.0 mL/min. A UV detector was operated at 280 nm and the calibration curves were linear over a concentration range of 2.50–7.50 µg/mL. The recovery values were calculated to be 99.13–100.34%. This method was successfully applied to quantify IPN HCl in injectable solutions. In addition, the method was validated according to the requirements of ICH guidelines. The proposed method for the determination of IPN was applied to pure and pharmaceutical preparations. Optimized geometric structures were drawn with GaussView 5. Then, the optimum geometric structures with the lowest energy were found with the Gaussian 09 program. These results will determine the most appropriate reaction pathways. Fragmentation patterns and degradation mechanisms initiated by hydroxyl radical attacks were evaluated in both gas and aqueous phases, incorporating solvation effects using the COSMO model. The molecular interactions of isoprenaline with β-adrenergic receptors were studied by molecular docking calculations and MD minimization in comparison with the natural ligands of these receptors, epinephrine and norepinephrine.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"89 1","pages":"61 - 77"},"PeriodicalIF":1.3,"publicationDate":"2025-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145983242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibodies to Carbohydrates, Affinity Purification with Milk Glycoproteins 碳水化合物抗体，乳糖蛋白亲和纯化

IF 1.3 4区化学

Chromatographia Pub Date : 2025-11-24 DOI: 10.1007/s10337-025-04458-9

Varduhi A. Hovhannisyan, Gayane G. Poghosyan, Vardan K. Gasparyan

引用次数: 0

The Use of Porous Shell-Type Particles in the Separation of Phytopigments by HPLC 多孔壳型颗粒在高效液相色谱分离植物色素中的应用

IF 1.3 4区化学

Chromatographia Pub Date : 2025-11-24 DOI: 10.1007/s10337-025-04454-z

Vinnicius Ferraço Brant, Silvana Vianna Rodrigues

{"title":"The Use of Porous Shell-Type Particles in the Separation of Phytopigments by HPLC","authors":"Vinnicius Ferraço Brant, Silvana Vianna Rodrigues","doi":"10.1007/s10337-025-04454-z","DOIUrl":"10.1007/s10337-025-04454-z","url":null,"abstract":"<div><p>Chemotaxonomy, based on pigment markers, has significantly enhanced the monitoring efficiency of phytoplankton assemblages. The quality of the results from chemotaxonomy based on pigment profiles is directly related to the performance achieved in the chromatographic process. HPLC methods described in the literature differ in resolution, sensitivity, and rapidity on the basis of the type of stationary phase and mobile phase used. The objective of this work is to compare the chromatographic performance of a column with porous shell technology particles with a column with totally porous particles, in the separation of phytoplankton pigments by HPLC. To evaluate the efficiency of the chromatographic column, analyses were carried out using a mixed standard containing 26 pigments and revealed greater resolution and peak shape than those obtained with a column made up of totally porous particles. The efficiency of a column with porous shell particles in separating phytopigments was higher than that of a column with totally porous particles, showing greater resolution and symmetry of the chromatographic peaks, especially for the pairs diadinoxanthin/dinoxanthin, divinyl chlorophyll <i>b</i>/chlorophyll <i>b</i> and divinyl chlorophyll <i>a</i>/chlorophyll <i>a</i>. Studies of the reproducibility of the method were carried out using oceanic samples. The resolution of the pair divinyl chlorophyll <i>a</i>/chlorophyll <i>a</i>, an important cyanobacterial marker, was two times higher in the porous shell column, reducing errors in the quantification of important taxonomic markers and securing the success of pigment-based chemotaxonomy.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"89 1","pages":"45 - 55"},"PeriodicalIF":1.3,"publicationDate":"2025-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145983227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Cationic Polyelectrolytes for Achiral and Chiral Separations in Capillary Electromigration Methods 毛细管电迁移法中用于手性和非手性分离的新型阳离子聚电解质

IF 1.3 4区化学

Chromatographia Pub Date : 2025-11-19 DOI: 10.1007/s10337-025-04447-y

Aleksandra Adamova, Daria Potapenko, Petr Fetin, Liudmila Kartsova

{"title":"New Cationic Polyelectrolytes for Achiral and Chiral Separations in Capillary Electromigration Methods","authors":"Aleksandra Adamova, Daria Potapenko, Petr Fetin, Liudmila Kartsova","doi":"10.1007/s10337-025-04447-y","DOIUrl":"10.1007/s10337-025-04447-y","url":null,"abstract":"<div><p>New polymeric modifiers of electrophoretic systems have been synthesized. These are cationic polyelectrolytes with micellar properties: poly-11-acryloyloxyundecyl-1,4-diazobicyclo[2.2.2]octanium bromide and a chiral copolymer based on acylated quinine and N-(11-acryloyloxydecyl)-N-methylpiperidinium bromide. The electrophoretic capabilities of these polymers have been studied in the separation of model mixtures of steroid hormones and biogenic amines by capillary micellar electrokinetic chromatography and capillary electrochromatography methods. It has been found that the new polyelectrolytes serve as multifunctional modifiers of the bare fused silica capillary wall and background electrolyte, allow for the implementation of various mechanisms of electrophoretic and chromatographic separation, generate an anodic electroosmotic flow, and influence the separation efficiency and selectivity. It was discovered that the copolymer with quinine acting as a chiral label in the composition of two-phase chiral systems promotes the separation of the enantiomers of β-blockers propranolol and carvedilol.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"89 1","pages":"35 - 43"},"PeriodicalIF":1.3,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145982964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an Improved LC–MS/MS Assay for the Quantification of Oximes in KIKO Mouse Plasma 一种改进的LC-MS /MS法定量KIKO小鼠血浆中氧肟的建立

IF 1.3 4区化学

Chromatographia Pub Date : 2025-11-17 DOI: 10.1007/s10337-025-04455-y

Katie A. Walker, Justin N. Vignola, C. Linn Cadieux, Robert C. diTargiani

{"title":"Development of an Improved LC–MS/MS Assay for the Quantification of Oximes in KIKO Mouse Plasma","authors":"Katie A. Walker, Justin N. Vignola, C. Linn Cadieux, Robert C. diTargiani","doi":"10.1007/s10337-025-04455-y","DOIUrl":"10.1007/s10337-025-04455-y","url":null,"abstract":"<div><p>Oxime reactivators are a part of the standard treatment for chemical warfare nerve agent exposure. Evaluating oxime candidates of interest in biological samples requires analytical detection methods, but oximes as a class of compounds have historically been difficult to isolate, detect, and analyze using conventional analytical techniques. Our lab previously developed novel extraction and liquid chromatography-tandem mass spectrometry (LC–MS/MS) methods to detect and quantitate 2-PAM, HI-6, HLö-7, and MMB-4 in human acetylcholinesterase knock-in, mouse carboxylesterase knock-out (KIKO) mouse plasma. These methods were validated to meet the Food and Drug Administration bioanalytical method validation requirements under Good Laboratory Practice conditions and then were utilized to analyze pharmacokinetic samples from KIKO mice. However, the future utility of these methods has been limited by the lack of reproducibly filled liquid chromatography (LC) columns. Herein we present an alternative LC column for the analysis of these oximes. Previously validated assay methods for oxime extraction from KIKO mouse plasma were evaluated for performance when analyzed by the new LC–MS/MS methodologies. Assays were evaluated for sensitivity, linearity, precision, accuracy, selectivity, and specificity. The results demonstrate that for 2-PAM, HI-6, and HLö-7, there was no change in assay performance when analyzed with the new LC–MS/MS methodologies. However, while MMB-4 was able to be retained on the new column, the sensitivity and linear range were not maintained from the original method. This study evaluates the potential of this alternative column to address inter-lot variability challenges, ensuring the robustness of oxime analytical methods.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"89 1","pages":"25 - 34"},"PeriodicalIF":1.3,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10337-025-04455-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145983055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Validation of RP-HPLC Method for Simultaneous Determination of Decitabine and Repaglinide: Application to Solid Lipid Nanoparticle Formulations with In Vitro Neurotoxicity Assessment 反相高效液相色谱法同时测定地西他滨和瑞格列奈：应用于固体脂质纳米颗粒制剂的体外神经毒性评估

IF 1.3 4区化学

Chromatographia Pub Date : 2025-11-16 DOI: 10.1007/s10337-025-04453-0

Shobha Kumari, Shiva Kumar Gogikar, Amol G. Dikundwar, Sunil Kumar Dubey, Rajeev Taliyan

{"title":"Development and Validation of RP-HPLC Method for Simultaneous Determination of Decitabine and Repaglinide: Application to Solid Lipid Nanoparticle Formulations with In Vitro Neurotoxicity Assessment","authors":"Shobha Kumari, Shiva Kumar Gogikar, Amol G. Dikundwar, Sunil Kumar Dubey, Rajeev Taliyan","doi":"10.1007/s10337-025-04453-0","DOIUrl":"10.1007/s10337-025-04453-0","url":null,"abstract":"<div><p>Parkinson's disease (PD) causes dopaminergic neuron loss and metabolic and epigenetic abnormalities. DNMT inhibitor decitabine (DB) promotes neuroprotective genes repressed by methylation. Repaglinide (RP), an insulin secretagogue used in T2DM, a PD comorbidity, reduces oxidative stress and inflammation by improving insulin signaling and regulating SIRT1 and HDACs. Bioavailability and half-life are issues for both drugs. Our innovative RP-HPLC technology and solid lipid nanoparticle (SLN) system for dual-drug administration enable prolonged release, brain targeting, and better pharmacokinetics for effective PD treatment. DB and RP were separated using a Waters™ e2695 HPLC system with a PDA detector and a Phenomenex Luna PFP column (250 × 4.6 mm, 5 µm) for enhanced selectivity. A 40:60 isocratic mobile phase (Solvent A:B) was optimized for peak shape and resolution. DB and RP were eluted at 2.7 and 3.6 min, respectively, throughout a 6-min run. The approach enabled precise, consistent, contemporaneous detection of both analytes for high-throughput analysis. Following conventional recommendations, the technique was validated for appropriateness, specificity, linearity, precision, accuracy, and robustness. The validated approach determined 53.26% and 76.68% drug entrapment efficiency in DB and RP SLN formulations. The MTT assay showed that DB and RP preserved N2A cell viability, signifying neuroprotective effects. The results confirmed the method's reliability, making it suitable for other applications, such as the simultaneous detection and quantification of these drugs in liposomal systems, polymer–lipid hybrid nanoparticles, and lipid–drug conjugates for in vitro and in vivo release and entrapment studies.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"89 1","pages":"11 - 24"},"PeriodicalIF":1.3,"publicationDate":"2025-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145983025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Why Settle for One Column if You Can Have Five?—Method Development for the Separation of Vitamin A Acetate Isomers Utilizing Stationary Phase Optimized Selectivity Liquid Chromatography 如果你可以有五个专栏，为什么还要满足于一个专栏？-利用固定相优化选择性液相色谱分离维生素A醋酸酯异构体的方法

IF 1.3 4区化学

Chromatographia Pub Date : 2025-11-07 DOI: 10.1007/s10337-025-04448-x

Leonard Schwaiger, Alexander Jaekel, Mo Legelli, Daniel Meyer, Stefan Lamotte, Michaela Wirtz

{"title":"Why Settle for One Column if You Can Have Five?—Method Development for the Separation of Vitamin A Acetate Isomers Utilizing Stationary Phase Optimized Selectivity Liquid Chromatography","authors":"Leonard Schwaiger, Alexander Jaekel, Mo Legelli, Daniel Meyer, Stefan Lamotte, Michaela Wirtz","doi":"10.1007/s10337-025-04448-x","DOIUrl":"10.1007/s10337-025-04448-x","url":null,"abstract":"<div><p>This study presents the development and optimization of a robust high-performance liquid chromatography (HPLC) method for the separation and detection of vitamin A acetate (VAA) isomers. The analytical strategy is based on stationary phase optimized selectivity liquid chromatography (SOSLC), utilizing a modular reversed-phase (RP) column setup. A range of stationary phases (SPs), including C18 strong hydrophobic (SH), C18 enhanced polar selectivity (EPS), C30, phenyl, and cyano columns were systematically evaluated to construct an optimized composite column with enhanced selectivity. Method development was guided by an existing chiral RP-HPLC gradient method, which served as a conceptual foundation and was adapted into an isocratic format to better accommodate differences in retention behavior and improve robustness. The resulting SOSLC-based HPLC method offers improved selectivity and robustness for the qualitative analysis of VAA isomers, supported by a gentle gradient program to decrease runtimes and sharpen late eluting analyte bands. This approach enables more reliable monitoring of supplement composition and lays the groundwork for future identification and structural elucidation of largely unexplored VAA oxidation products, with initial MS/MS results indicating further potential for analyte characterization.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"89 1","pages":"1 - 10"},"PeriodicalIF":1.3,"publicationDate":"2025-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145982434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obituary: Dr. Terry Alan Berger (November 14, 1946–May 5, 2024) 讣告：特里·艾伦·伯杰博士（1946年11月14日- 2024年5月5日）

IF 1.3 4区化学

Chromatographia Pub Date : 2025-11-06 DOI: 10.1007/s10337-025-04445-0

引用次数: 0

Online Comprehensive Two-Dimensional Normal-Phase × Reversed-Phase Preparative Liquid Chromatography System with a Novel Design Interface 一种新型设计界面的在线二维正相×反相制备液相色谱系统

IF 1.3 4区化学

Chromatographia Pub Date : 2025-11-02 DOI: 10.1007/s10337-025-04446-z

Su-Zhi Xie, Mei-Juan Fang, Zheng Wang, Xia Yan, Ying-Kun Qiu

引用次数: 0