Chromatographia最新文献_第10页

Tandem-Column High Performance Liquid Chromatography Separation of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) 串联柱高效液相色谱法分离非甾体抗炎药

IF 1.7 4区化学

Chromatographia Pub Date : 2023-10-27 DOI: 10.1007/s10337-023-04286-9

Megan E. Marrazzo, Joe P. Foley

{"title":"Tandem-Column High Performance Liquid Chromatography Separation of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs)","authors":"Megan E. Marrazzo, Joe P. Foley","doi":"10.1007/s10337-023-04286-9","DOIUrl":"10.1007/s10337-023-04286-9","url":null,"abstract":"<div><p>A tandem-column liquid chromatographic method was developed to separate ten non-steroidal anti-inflammatory drugs (Aceclofenac, Aspirin, Carprofen, Celecoxib, Diclofenac, Flurbiprofen, Ibuprofen, Ketoprofen, Naproxen, Salicylic Acid) using an achiral tandem-column configuration under reversed-phase conditions. An NSAID test mix was prepared with 0.5 mg/mL thiourea as a t<sub>0</sub> marker and 0.5 mg/mL of the above NSAIDs in 1:1 acetonitrile: 0.2% formic acid. Three different 5-cm columns were investigated individually and in two different tandem-column configurations: an octyl (C8) column, a perfluorophenyl (PFP) column, and a biphenyl (BP) column. The two tandem configurations investigated were a PFP column followed by either a BP or C8 column. While none of the individual columns provided the selectivity needed to separate two sets of critical pairs (Ketoprofen and Naproxen; Flurbiprofen and Ibuprofen), the unique selectivity provided by the two tandem-column combinations enabled both critical pairs to be separated. The PFP + BP tandem column combination was able to separate the 10 NSAIDs with baseline or greater resolution in less than 25 min.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"86 11-12","pages":"743 - 750"},"PeriodicalIF":1.7,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71910759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality Evaluation of Sanguisorbae Radix via Python Aided Optimization Fingerprint Chromatography Combined with Quantitative Analysis of Multi-components by Single Marker Python辅助优化指纹图谱结合单标记多组分定量分析评价地榆的质量

IF 1.7 4区化学

Chromatographia Pub Date : 2023-10-26 DOI: 10.1007/s10337-023-04284-x

Yuan Gao, Bin Qiao, Zarmina Gul, Mengfei Tian, Jiabo Cheng, Chunguo Xu, Chunjian Zhao, Chunying Li

{"title":"Quality Evaluation of Sanguisorbae Radix via Python Aided Optimization Fingerprint Chromatography Combined with Quantitative Analysis of Multi-components by Single Marker","authors":"Yuan Gao, Bin Qiao, Zarmina Gul, Mengfei Tian, Jiabo Cheng, Chunguo Xu, Chunjian Zhao, Chunying Li","doi":"10.1007/s10337-023-04284-x","DOIUrl":"10.1007/s10337-023-04284-x","url":null,"abstract":"<div><p>A simple method was proposed to assess the quality of <i>Sanguisorbae Radix</i> based on Python-aided optimization fingerprint chromatography (FC) combined with quantitative analysis of multi-components by single marker (PA-FC/QAMS). The Python program quickly anticipated the mobile phase conditions for fingerprints, and the result yielded a chromatogram with good peak and resolution. The estimated chromatogram was similar to that obtained from the actual experiment. Furthermore, we used the mobile phase conditions predicted by Python to establish a fingerprint of 15 batches of <i>Sanguisorbae Radix</i>. and optimized it. Compared with the traditional trial-and-error method used to optimize the mobile phase conditions during the experiment, the efficient Python prediction method substantially reduced the number of experiments needed for optimum mobile phase conditions. In addition, exclusive mobile phase composition can be simulated according to different experimental instruments and chromatographic columns. Moreover, 15 batches of samples from different regions were classified by similarity analysis, cluster analysis, and factor analysis. This study shows that PA-FC/QAMS method can provide a simple and efficient approach for the quality evaluation of <i>Sanguisorbae Radix</i>.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"86 11-12","pages":"717 - 727"},"PeriodicalIF":1.7,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71910800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Congress, Conferences, and Workshops 大会、会议和研讨会

IF 1.7 4区化学

Chromatographia Pub Date : 2023-10-25 DOI: 10.1007/s10337-023-04288-7

引用次数: 0

ChemTexts, The Textbook Journal of Chemistry 化学教科书杂志ChemTexts

IF 1.7 4区化学

Chromatographia Pub Date : 2023-10-17 DOI: 10.1007/s10337-023-04287-8

Claudia Zielke

引用次数: 0

Glycerol-Based Green RP-HPLC Method for Simultaneous Determination of Methionine and Paracetamol in Pharmaceutical Tablets 甘油绿色RP-HPLC法同时测定片剂中蛋氨酸和对乙酰氨基酚的含量

IF 1.7 4区化学

Chromatographia Pub Date : 2023-10-10 DOI: 10.1007/s10337-023-04283-y

Ahmed Habib, Mokhtar M. Mabrouk, Marwa Fekry, Fotouh R. Mansour

{"title":"Glycerol-Based Green RP-HPLC Method for Simultaneous Determination of Methionine and Paracetamol in Pharmaceutical Tablets","authors":"Ahmed Habib, Mokhtar M. Mabrouk, Marwa Fekry, Fotouh R. Mansour","doi":"10.1007/s10337-023-04283-y","DOIUrl":"10.1007/s10337-023-04283-y","url":null,"abstract":"<div><p>Methionine and paracetamol are combined in dosage forms due to the ability of methionine to compensate the deficiency in glutathione in hepatic patients. In this work, an innovative simple time-saving green RP-HPLC method using glycerol as a solely green mobile phase with water for the first time was developed for the simultaneous determination of methionine and paracetamol in their standard and in Hepamol® tablets. The chromatographic conditions were optimized using factorial design with the aid of Minitab 17® Software. The method was performed on a C18 column at 38 °C, and a mobile phase consisting of glycerol and phosphate buffer (pH 2.4) (40:60, v/v), using diode array detector at 210 nm. The mixture was separated in 5 min. The developed method was validated in accordance with ICH requirement over linearity ranges of 10–90 μg/mL for both drugs, and LODs were 3.33 μg/mL for both methionine and paracetamol. Glycerol has high safety, low UV cut-off point, low flammability, and its viscosity can be manipulated when diluted with water. The procedure was compared to the reported reversed phase liquid chromatography method in the terms of their greenness with the green analytical procedure index and the analytical eco-scale. This work breaks new ground for scientists to use glycerol in greener RP-HPLC applications.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"86 11-12","pages":"707 - 716"},"PeriodicalIF":1.7,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10337-023-04283-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71909795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A Sensitive Online SPE-LC–APCI–MS/MS Method for Simultaneous Determination of 17 Nitrated and Oxygenated Polycyclic Aromatic Hydrocarbons in Atmospheric Particulate Matter 灵敏在线SPE-LC–APCI–MS/MS法同时测定大气颗粒物中17种硝化和氧化多环芳烃

IF 1.7 4区化学

Chromatographia Pub Date : 2023-10-05 DOI: 10.1007/s10337-023-04280-1

Chao Wang, Weihong Zhu, Zongbo Shi, Jianjun Li

{"title":"A Sensitive Online SPE-LC–APCI–MS/MS Method for Simultaneous Determination of 17 Nitrated and Oxygenated Polycyclic Aromatic Hydrocarbons in Atmospheric Particulate Matter","authors":"Chao Wang, Weihong Zhu, Zongbo Shi, Jianjun Li","doi":"10.1007/s10337-023-04280-1","DOIUrl":"10.1007/s10337-023-04280-1","url":null,"abstract":"<div><p>A highly sensitive method was developed for the determination of 17 nitrated and oxygenated polycyclic aromatic hydrocarbons (NPAHs and OPAHs) in PM<sub>2.5</sub> using an ultrasound-assisted extraction (UAE)-online solid phase extraction (online SPE)-liquid chromatography-tandem mass spectrometry (LC/MS). The method underwent comprehensive optimization, encompassing UAE solvents and times, extract filtering, online SPE condition and LC/MS condition. Isomeric separation and detection of mononitro-PAHs, dinitro-PAHs, and OPAHs were achieved based on the proposed MS/MS fragmentation pathway in negative mode atmospheric pressure chemical ionization. Taking the advantage of online SPE, the method achieved lower limits of detection (NPAHs: 0.001 ~ 0.042 μg L<sup>−1</sup>, OPAHs: 0.01 ~ 0.038 μg L<sup>−1</sup>). Recovery ranged from 64% ~ 108% at three spiking levels (0.25, 1.0 and 10.0 μg L<sup>−1</sup>) with the relative standard deviations (RSDs) of 3% ~ 23%. This study represents first application of LC/MS methodology for the quantification of three carcinogenic dinitro-PAHs (1,3-dinitropyrene, 1,6-dinitropyrene, 1,8-dinitropyrene) in atmospheric PM<sub>2.5</sub>. The established method was successfully employed to determine the wide range concentrations of various NPAHs and OPAHs (∑NPAH: 9.4 ~ 303.3 pg m<sup>−3</sup>, ∑OPAHs: 125.2 ~ 1948.1 pg m<sup>−3</sup>) in PM<sub>2.5</sub> samples collected in Beijing during the summer and winter seasons.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"86 10","pages":"677 - 688"},"PeriodicalIF":1.7,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41181122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiple Response Optimization of an Ionic Liquid-DLLME and UHPLC-DAD Analysis of Five Coccidiostats in the Chicken Liver with LC–MS/MS Confirmation 离子液体DLLME和UHPLC-DAD的多重响应优化鸡肝中五种球虫抑制剂的LC-MS/MS确证分析

IF 1.7 4区化学

Chromatographia Pub Date : 2023-10-04 DOI: 10.1007/s10337-023-04281-0

Veena Jain, Somendu Kumar Roy

{"title":"Multiple Response Optimization of an Ionic Liquid-DLLME and UHPLC-DAD Analysis of Five Coccidiostats in the Chicken Liver with LC–MS/MS Confirmation","authors":"Veena Jain, Somendu Kumar Roy","doi":"10.1007/s10337-023-04281-0","DOIUrl":"10.1007/s10337-023-04281-0","url":null,"abstract":"<div><p>Pharmaceutical residues in animal food products from various sources are a crucial concern regarding food safety measures for human welfare. Coccidiostats are predominantly used in the poultry industry to prevent protozoal infection. Excessive use and slaughter before completion of withdrawal periods of drugs are likely to present residues in poultry products. As a result, humans may be exposed to these drugs through the consumption of poultry products. Therefore, we developed an ionic liquid-dispersive liquid‒liquid microextraction (IL-DLLME)-based method to evaluate the drug residues of five coccidiostats in chicken liver using UHPLC-PDA. The IL-DLLME extraction procedure was optimized using an experimental approach that included screening design, response surface methodology (RSM), and desirability function. After optimizing the necessary conditions, good linearity was achieved between 39 and 1250 ngmL<sup>−1</sup> with a coefficient of determination (<i>R</i><sup>2</sup>) ≥ 0.999. The upgraded method showed excellent precision with a relative standard deviation (%RSD) ≥ 0.30–6.53% and significant recovery in the range of 61.57–107.49%. The limits of detection (LODs) and limits of quantitation (LOQs) for the five coccidiostats (UHPLC-PDA) ranged from 3.1 to 8.7 ngmL<sup>−1</sup> and 10 to 29 ngmL<sup>−1</sup>, respectively. The developed method was applied to examine coccidiostats in chicken liver samples purchased from a local market. In addition, LC‒MS/MS analysis confirmed the presence of traces in liver samples. Thus, the developed method of extraction is precise and reproducible.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"86 10","pages":"689 - 699"},"PeriodicalIF":1.7,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41181147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Determination of Ruscogenin, Neoruscogenin, Trimebutin, and Parabens in Cream Formulation by Reverse Phase High Performance Liquid Chromatography 反相高效液相色谱法同时测定乳膏中的Ruscocin、Neoruscocin，Trimebutin和Parabens

IF 1.7 4区化学

Chromatographia Pub Date : 2023-10-04 DOI: 10.1007/s10337-023-04282-z

Gürkan Özen, Emirhan Nemutlu

{"title":"Simultaneous Determination of Ruscogenin, Neoruscogenin, Trimebutin, and Parabens in Cream Formulation by Reverse Phase High Performance Liquid Chromatography","authors":"Gürkan Özen, Emirhan Nemutlu","doi":"10.1007/s10337-023-04282-z","DOIUrl":"10.1007/s10337-023-04282-z","url":null,"abstract":"<div><p>A simple and stability indicating reverse-phase high-performance liquid chromatographic method for the simultaneous analysis of ruscogenin, neoruscogenin, trimebutine, and parabens including methylparaben and propylparaben has been developed and validated. The separations were performed using a C18 column (250 × 4.6 mm, 5 μm i.d.). The column temperature was set to 25 °C. The mobile phase consisted of acetonitrile (mobile phase B) and 20 mM phosphate buffer pH 3.9 (mobile phase A) and was mixed in gradient elution mode using a binary pump. The developed method was validated according to the requirements of the ICH Q2(R2) guideline. In all cases, the method was found selective for all active substances and parabens. The limit of detection and limit of quantification values ranged from 0.02 to 0.45 µg mL<sup>–1</sup> and from 0.06 to 1.35 µg mL<sup>–1</sup>, respectively. The intraday and interday accuracy (relative error ≤ 1.89) and precision (relative standard deviation ≤ 1.99) values of the method for all compounds were found in the limits. Based on the validation studies, the developed method was found to be selective (peak purity index ≥ 0.9904), sensitive, accurate, precise, and robust. The developed method was successfully applied for the quantification of Ruscogenin, neoruscogenin, trimebutine and methylparaben, propylparaben from the semi-solid dosage form.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"86 10","pages":"649 - 657"},"PeriodicalIF":1.7,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41181159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosilica as a New Stationary Phase in HILIC Mode 生物二氧化硅作为HILIC模式下的新固定相

IF 1.7 4区化学

Chromatographia Pub Date : 2023-09-26 DOI: 10.1007/s10337-023-04275-y

Hussam A. Al Saoud, Michał Szumski, Myroslav Sprynskyy, Szymon Bocian, Bogusław Buszewski

引用次数: 0

The Innovative Applications of Carbon Nanomaterials, Nanocomposites, and Related Carbon-Based Materials in Separation Science 碳纳米材料、纳米复合材料及相关碳基材料在分离科学中的创新应用

IF 1.7 4区化学

Chromatographia Pub Date : 2023-09-16 DOI: 10.1007/s10337-023-04278-9

Brett Paull

引用次数: 0