Chromatographia最新文献_第10页

Study on the Combination of Multi-wavelength Calibration and Liquid Chromatography with UV–Vis Detector for the Determination of the Synthetic Dye Sunset Yellow FCF in Soft Drinks 将多波长校准和带紫外可见检测器的液相色谱法结合起来测定软饮料中合成染料日落黄 FCF 的研究

IF 1.2 4区化学

Chromatographia Pub Date : 2023-12-30 DOI: 10.1007/s10337-023-04305-9

Jhonatan Bispo de Oliveira, Ludmilla Sousa Lopes, Fernando César da Costa, Gregorio Morais Saravia, Ingrid Amélia dos Santos Matusinho

{"title":"Study on the Combination of Multi-wavelength Calibration and Liquid Chromatography with UV–Vis Detector for the Determination of the Synthetic Dye Sunset Yellow FCF in Soft Drinks","authors":"Jhonatan Bispo de Oliveira, Ludmilla Sousa Lopes, Fernando César da Costa, Gregorio Morais Saravia, Ingrid Amélia dos Santos Matusinho","doi":"10.1007/s10337-023-04305-9","DOIUrl":"10.1007/s10337-023-04305-9","url":null,"abstract":"<div><p>Calibration is a fundamental step in instrumental quantitative methods that produce an analytical signal proportional to the concentration of the analyte in the sample. Multi-wavelength calibration (MWC), a novel calibration strategy, has been proposed as a simple method for the determination of analytes in samples with complex matrices. It has minimal matrix effects and straightforward sample preparation. The objective of this work was to apply this calibration strategy to the determination of the synthetic dye Sunset Yellow in soft drinks by high-performance liquid chromatography with ultraviolet–visible analysis (HPLC/UV–Vis). Multi-wavelength calibration planning was performed as recommended in the literature. MWC results were statistically compared with conventional external calibration (limit of detection, limit of quantification, linearity, repeatability and recovery). The results obtained with both calibrations showed that the soft drinks complied with Brazilian legislation. The dye contents obtained with both calibration methods did not show significant statistical differences. MWC appears as an alternative or complement to external calibrations and ensures minimal matrix effects and straightforward sample preparation.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 2","pages":"137 - 144"},"PeriodicalIF":1.2,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139064980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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An UHPLC-QE-Orbitrap-MS Method for Accurate Quantification of Short-Chain Fatty Acids in Serum From an Older Chinese Population 超高效液相色谱-QE-轨道阱-质谱法精确定量中国老年人血清中的短链脂肪酸

IF 1.2 4区化学

Chromatographia Pub Date : 2023-12-22 DOI: 10.1007/s10337-023-04304-w

Sheng Wang, Rui Feng, Li Kong, Rui Zhou, Fang-ting Hu, Shu-Jing Sun, Guan-Jun Chen, Fang-Biao Tao, Kai-Yong Liu

{"title":"An UHPLC-QE-Orbitrap-MS Method for Accurate Quantification of Short-Chain Fatty Acids in Serum From an Older Chinese Population","authors":"Sheng Wang, Rui Feng, Li Kong, Rui Zhou, Fang-ting Hu, Shu-Jing Sun, Guan-Jun Chen, Fang-Biao Tao, Kai-Yong Liu","doi":"10.1007/s10337-023-04304-w","DOIUrl":"10.1007/s10337-023-04304-w","url":null,"abstract":"<div><p>Short-chain fatty acids (SCFAs), such as acetic acid, propionic acid, lactic acid, β-hydroxybutyric acid, and crotonic acid, play key biological roles and are also strongly associated with the maintenance of health and the development of age-related diseases. However, an accurate method for SCFAs detection in human serum is lacking. Herein, we developed an UHPLC-QE-Orbitrap MS method based on 3-nitrophenylhydrazine derivatization in negative electrospray ionization through parallel reaction monitoring mode for the simultaneous detection of 11 SCFAs in the serum, and the analysis was performed on an Agilent Proshell 120 EC-C<sub>18</sub> column (2.1 mm × 100 mm, 2.7 μm). Three pairs of isomers—isobutyric and butyric acid, isovaleric and valeric acid, and isocaproic and caproic acid—were completely separated in 20 min in a single run. Our method exhibited satisfactory linearity (<i>R</i> > 0.99) for all analytes, and both intra-day and inter-day accuracies (73.74% to 127.9%) and precisions ( < 21%) were acceptable for most targeted compounds. The extraction recoveries ranged from 90.80% to 111.7%, and the internal standard-normalized matrix effects were 74.43%–116.9%. This method was successfully applied to a cohort of 1021 older Chinese individuals. Our results may further the understanding of the metabolic phenotypes associated with SCFAs in other populations.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 2","pages":"125 - 136"},"PeriodicalIF":1.2,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138946982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virtual Issue: New Modified Silica Stationary Phases 虚拟期刊：新型改性二氧化硅固定相

IF 1.2 4区化学

Chromatographia Pub Date : 2023-12-08 DOI: 10.1007/s10337-023-04302-y

Arianne Soliven

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Congress, Conferences, and Workshops 大会、会议和研讨会

IF 1.2 4区化学

Chromatographia Pub Date : 2023-12-07 DOI: 10.1007/s10337-023-04303-x

引用次数: 0

A Method to Externally Adjust the Column Length in Gas Chromatography Using a Water Stationary Phase 使用水固定相外部调节气相色谱柱长度的方法

IF 1.2 4区化学

Chromatographia Pub Date : 2023-12-06 DOI: 10.1007/s10337-023-04299-4

Kade L. Shepherd, Kevin B. Thurbide

{"title":"A Method to Externally Adjust the Column Length in Gas Chromatography Using a Water Stationary Phase","authors":"Kade L. Shepherd, Kevin B. Thurbide","doi":"10.1007/s10337-023-04299-4","DOIUrl":"10.1007/s10337-023-04299-4","url":null,"abstract":"<div><p>A novel method for externally adjusting the column length during gas chromatography (GC) operation is introduced. The technique employs the controlled dehydration of a water stationary phase off a stainless-steel capillary column wall, which is then removed by the carrier gas. By halting the dehydration process (i.e. through adding water to the system) at specified times, partial column (i.e. coating) lengths are created as desired. For instance, since the phase is removed in a ‘peeling’ motion from inlet to outlet, then dehydrating 1/2 of the water coating away results in a 1/2 coated column remaining. This, in turn, acts analogous to a 1/2 column length in separations. In this way, direct and effective reduction in analyte retention time results from shortening column length. Adjustable lengths from 3/4 down to 1/10 of a full column are demonstrated. Good stability in maintaining new column lengths is realized, as analyte retention times vary by only about 1% RSD for most lengths and temperatures examined. Through calibration, column length can be predictably adjusted using system dehydration time, with changes being performed in a few minutes. Results indicate that this method could be useful for adjusting column length <i>in-situ</i> to accommodate various samples during GC operation.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 2","pages":"87 - 93"},"PeriodicalIF":1.2,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138546999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of New 4-Aryl-pyrido[1,2-c]pyrimidine Derivatives, Potential Antidepressant Agents with a High Affinity to 5-Hydroxytryptamin 1A Receptor and Serotonin Transporter Protein Receptor, with Capillary Electrophoresis 新4-芳基吡啶[1,2-c]嘧啶衍生物与5-羟色胺1A受体和5-羟色胺转运蛋白受体高亲和力的潜在抗抑郁药物的毛细管电泳测定

IF 1.2 4区化学

Chromatographia Pub Date : 2023-12-04 DOI: 10.1007/s10337-023-04300-0

Błażej Grodner, Marek Król, Grzegorz Ślifirski, Piotr Ślifirski, Franciszek Herold

{"title":"Determination of New 4-Aryl-pyrido[1,2-c]pyrimidine Derivatives, Potential Antidepressant Agents with a High Affinity to 5-Hydroxytryptamin 1A Receptor and Serotonin Transporter Protein Receptor, with Capillary Electrophoresis","authors":"Błażej Grodner, Marek Król, Grzegorz Ślifirski, Piotr Ślifirski, Franciszek Herold","doi":"10.1007/s10337-023-04300-0","DOIUrl":"10.1007/s10337-023-04300-0","url":null,"abstract":"<div><p>A novel capillary electrophoresis method was developed for the determination of new 4-aryl-pyrido[1,2-c]pyrimidine derivatives, potential antidepressant agents, in serum. The derivatives have conformationally restricted tryptamine moiety in pharmacophore portion and exhibit high affinity to molecular targets: 5-HT<sub>1A</sub> receptor and serotonin transporter protein. The separation process was conducted using an eCAP fused-silica capillary, detection wavelength 214 nm, 200 mM phosphate buffer adjusted to pH = 8.0, temperature 20 °C, voltage 5 kV. The proposed method was validated by determining its linearity in the concentration range of 200–1000 ng/mL. A satisfactory linearity was obtained for the method, with R<sup>2</sup> from 0.9978 to 0.9999 for all five derivatives and a limit of quantification level from 287.1 to 310.1 ng/mL. The recoveries for all derivatives were in the range from 94.7 to 100%. The speed of obtaining the result of the analysis was only 3 min. The developed method allows to determine all five derivatives both in water solutions and serum.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 2","pages":"117 - 124"},"PeriodicalIF":1.2,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10337-023-04300-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138506487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Certified Reference Material of Polyvinyl Chloride Containing Phthalic Esters (NMIJ CRM 8156-a) for Screening Device Applications 一种经认证的含邻苯二甲酸酯聚氯乙烯标准物质(NMIJ CRM 8156-a)，用于筛选设备应用

IF 1.2 4区化学

Chromatographia Pub Date : 2023-11-30 DOI: 10.1007/s10337-023-04297-6

Nobuyasu Hanari, Yukari Orihara, Yoshie Aoyagi, Shigetomo Matsuyama

{"title":"A Certified Reference Material of Polyvinyl Chloride Containing Phthalic Esters (NMIJ CRM 8156-a) for Screening Device Applications","authors":"Nobuyasu Hanari, Yukari Orihara, Yoshie Aoyagi, Shigetomo Matsuyama","doi":"10.1007/s10337-023-04297-6","DOIUrl":"10.1007/s10337-023-04297-6","url":null,"abstract":"<div><p>Phthalic esters (PEs) are used as plasticizers for plastic products especially for polyvinyl chloride (PVC). Owing to their adverse effects on human health, certain types of PEs are subjected to regulatory measures. Accurate and precision analyses of such regulated PEs are essential for monitoring compliance with regulatory standards; thus, the use of certified reference materials (CRMs) for quality assurance is recommended. To quantify the PE content of PVC products, the National Metrology Institute of Japan has developed a CRM, designated as NMIJ CRM 8156-a. This CRM is designed for validating the analytical methods and screening devices at low concentration levels (ca. 0.01%). Isotope-dilution mass spectrometry, the primary methods of measurement, has been employed for certification in conjunction with optimized gas and liquid chromatography/mass spectrometry techniques. Based on the mean mass fractions of target PEs derived from these two methods, eight certified values were determined for the CRM. The uncertainties associated with these certified values were estimated considering various factors such as characterization, homogeneity, and stability. Furthermore, the NMIJ CRMs were tested in screening devices such as pyrolyzers and thermal desorption mass spectrometers, confirming their suitability for generating calibration curves as well as the semi-quantitative analysis of target PEs. In summary, these CRMs are effective for validating quantitative and screening analytical methods for PEs in PVC and related substances.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 1","pages":"35 - 43"},"PeriodicalIF":1.2,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138506471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Coumarin-Based Phototoxins in Citrus-Derived Essential Oils Using Liquid Chromatography-Mass Spectrometry 液相色谱-质谱联用分析柑橘精油中香豆素类光毒素

IF 1.2 4区化学

Chromatographia Pub Date : 2023-11-29 DOI: 10.1007/s10337-023-04298-5

Siheng Li, Colleen Kelly, Radim Knob, Kent McConnell, Justin Barrett

{"title":"Analysis of Coumarin-Based Phototoxins in Citrus-Derived Essential Oils Using Liquid Chromatography-Mass Spectrometry","authors":"Siheng Li, Colleen Kelly, Radim Knob, Kent McConnell, Justin Barrett","doi":"10.1007/s10337-023-04298-5","DOIUrl":"10.1007/s10337-023-04298-5","url":null,"abstract":"<div><p>The occurrence of coumarin-based photoactive compounds in essential oils has been a concern due to the adverse health effects it has on human health. A fit-for-purpose analytical method for major coumarin-based phototoxins is critical to guarantee the essential oils product safety and compliance with the regulatory requirements. Herein, a liquid chromatography-mass spectrometry (LC–MS) analytical method was developed to identify and quantify 25 coumarin derivatives in essential oils. The sample preparation procedure was optimized to use selective solvent extraction to remove the limonene matrix interference. To achieve the optimal sensitivity and specificity, UHPLC column of biphenyl stationary phase was used for this analysis. An inter-laboratory validation study was conducted to evaluate the method performance. The limit of quantification (LOQ) for all target analytes was validated at 5 µg/g. Satisfactory recoveries were obtained for the majority of analytes in the range of 80–115%. Both the repeatability RSD<sub>r</sub> (within-laboratory) and reproducibility RSD<sub>R</sub> (between-laboratory) were < 10%. This method was demonstrated as applicable for the quality control testing of coumarin-based phototoxins in essential oils.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 1","pages":"59 - 69"},"PeriodicalIF":1.2,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138541916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Composition Analysis and Multi-index Component Content Determination of Compounds in Goupi Plaster Based on UPLC–Q-Exactive-MS and UPLC–MS/MS 基于uplc - q - hplc -MS和UPLC-MS /MS的狗皮膏化学成分分析及多指标成分含量测定

IF 1.2 4区化学

Chromatographia Pub Date : 2023-11-20 DOI: 10.1007/s10337-023-04296-7

Yunfeng Xue, Tong Guan, Jia Liu, Jiajing Wang, Zhixin Yang, Feng Guan, Weinan Li, Yanhong Wang

{"title":"Chemical Composition Analysis and Multi-index Component Content Determination of Compounds in Goupi Plaster Based on UPLC–Q-Exactive-MS and UPLC–MS/MS","authors":"Yunfeng Xue, Tong Guan, Jia Liu, Jiajing Wang, Zhixin Yang, Feng Guan, Weinan Li, Yanhong Wang","doi":"10.1007/s10337-023-04296-7","DOIUrl":"10.1007/s10337-023-04296-7","url":null,"abstract":"<div><h3>Objective</h3><p>This study sought to evaluate and validate a method for chemical composition analysis and content determination of Goupi plaster components, a conventional prescription preparation of traditional Chinese medicine. This is geared toward providing a basis for quality control research and future development of Goupi plaster.</p><h3>Methods</h3><p>UPLC–Q-Exactive-MS was used to qualitatively analyze the chemical components of Goupi plaster from different manufacturers in positive and negative ion modes. UPLC–MS/MS method was used to establish the determination methods for the detection of sinomenine, osthole, and cinnamaldehyde in Goupi plaster from different manufacturers.</p><h3>Results</h3><p>A total of 291 chemical components were identified in Goupi plaster from four manufacturers, including 97 chemical components with known source and pharmacological activity. Further, we determined the contents of sinomenine, osthole, and cinnamaldehyde.</p><h3>Conclusion</h3><p>In summary, the UPLC–Q-Exactive-MS method was used to analyze the chemical components of Goupi plaster from different manufacturers. We established the UPLC–MS/MS method to determine the contents of sinomenine, osthole, and cinnamaldehyde in Goupi plaster from different manufacturers. The findings indicated that the method was comprehensive, rapid, and accurate, preliminarily revealing the material basis of Goupi plaster and providing a reference for follow-up development of Goupi plaster.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 1","pages":"45 - 57"},"PeriodicalIF":1.2,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10337-023-04296-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138506476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Forced Degradation of an Anticancer Drug Apalutamide: Impurity Profiling and Structure Elucidation Study 抗癌药物阿帕鲁胺的强制降解：杂质分析和结构阐明研究

IF 1.2 4区化学

Chromatographia Pub Date : 2023-11-09 DOI: 10.1007/s10337-023-04294-9

Rekha Sapkal, Sadaf Quadri, Rupali Mahajan, Hara Prasad Padhy, Amit Asthana, Gananadhamu Samanthula, Amol G. Dikundwar

{"title":"Forced Degradation of an Anticancer Drug Apalutamide: Impurity Profiling and Structure Elucidation Study","authors":"Rekha Sapkal, Sadaf Quadri, Rupali Mahajan, Hara Prasad Padhy, Amit Asthana, Gananadhamu Samanthula, Amol G. Dikundwar","doi":"10.1007/s10337-023-04294-9","DOIUrl":"10.1007/s10337-023-04294-9","url":null,"abstract":"<div><p>An anticancer drug apalutamide approved by United States Food and Drug Administration in 2018 is one of the most commonly prescribed chemotherapeutic agents used for the treatment of prostate cancer. The present study aims at establishing the chemical structures of all the degradation products of the active pharmaceutical ingredient (API) formed under different forced degradation conditions. Apalutamide degradation was studied under International Council for Harmonisation recommended conditions of hydrolysis (acidic, alkaline, and neutral), oxidation, photolysis, and thermal stress. In total, seven degradation products (DP-1 to DP-7) were observed which were successfully separated on high-performance liquid chromatography and further characterized using liquid chromatography–mass spectrometry quadrupole time-of-flight (LC–MS/MS-QTOF). Degradation pathways for each of the observed DPs have been proposed based on the mass fragmentation pattern of API as well as DPs, identifying the underlying chemical transformations. Prediction of DPs was performed with Zeneth software tool, and the results were compared with the experimental observations. In silico toxicity assessment carried out using Derek suite reveals toxic nature of some of these DPs.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 1","pages":"71 - 82"},"PeriodicalIF":1.2,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135192572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0